Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34316.t1 | XP_013454489.1 | 100 | 70 | 0 | 0 | 1 | 70 | 72 | 141 | 3.40E-28 | 133.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34316.t1 | Q8GWH5 | 81.4 | 70 | 13 | 0 | 1 | 70 | 57 | 126 | 1.5e-24 | 112.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34316.t1 | A0A072UFI9 | 100.0 | 70 | 0 | 0 | 1 | 70 | 72 | 141 | 2.5e-28 | 133.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene34316 | -0.811598 | 6.46E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene34316 | -0.800113 | 1.68E-48 | -1.69E-46 |
| MS.gene050623 | MS.gene34316 | 0.813776 | 2.16E-51 | -1.69E-46 |
| MS.gene050762 | MS.gene34316 | -0.839244 | 1.78E-57 | -1.69E-46 |
| MS.gene050763 | MS.gene34316 | -0.839567 | 1.47E-57 | -1.69E-46 |
| MS.gene052970 | MS.gene34316 | 0.972862 | 1.83E-135 | -1.69E-46 |
| MS.gene054379 | MS.gene34316 | -0.825452 | 4.65E-54 | -1.69E-46 |
| MS.gene055413 | MS.gene34316 | -0.824979 | 6.02E-54 | -1.69E-46 |
| MS.gene058182 | MS.gene34316 | -0.805037 | 1.62E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene34316 | -0.839635 | 1.41E-57 | -1.69E-46 |
| MS.gene060297 | MS.gene34316 | -0.808515 | 2.98E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCTTGGAAGACTGGCTT+TGG | 0.289145 | 4.3:+89209703 | MS.gene34316:CDS |
| CTTTATGCATTATCTGCTCT+TGG | 0.374994 | 4.3:+89209689 | MS.gene34316:CDS |
| GTCTTGAGGGTTGCTGCTAC+TGG | 0.407570 | 4.3:+89209338 | MS.gene34316:CDS |
| GCTCTTGGAAGACTGGCTTT+GGG | 0.418794 | 4.3:+89209704 | MS.gene34316:CDS |
| TGCCACTCAAGGGGATCAAC+AGG | 0.434496 | 4.3:-89209248 | None:intergenic |
| TGCCGCTGAATCTGTCTTGA+GGG | 0.443932 | 4.3:+89209325 | MS.gene34316:CDS |
| CAAGAGCAGATAATGCATAA+AGG | 0.448013 | 4.3:-89209688 | None:intergenic |
| ATTATCTGCTCTTGGAAGAC+TGG | 0.464565 | 4.3:+89209697 | MS.gene34316:CDS |
| GGTATTACTATCGCCTATGC+TGG | 0.488512 | 4.3:+89209725 | MS.gene34316:CDS |
| GCTTCTTTCGCTAATACTGT+CGG | 0.494988 | 4.3:+89209302 | MS.gene34316:CDS |
| TTGAGATATTTGCCACTCAA+GGG | 0.504422 | 4.3:-89209258 | None:intergenic |
| CTTGAGATATTTGCCACTCA+AGG | 0.517790 | 4.3:-89209259 | None:intergenic |
| GTGCCGCTGAATCTGTCTTG+AGG | 0.538110 | 4.3:+89209324 | MS.gene34316:CDS |
| AGAAAGGTACGAACCAGCAT+AGG | 0.589035 | 4.3:-89209738 | None:intergenic |
| TGAGATATTTGCCACTCAAG+GGG | 0.608929 | 4.3:-89209257 | None:intergenic |
| TTCCTGTTGATCCCCTTGAG+TGG | 0.613108 | 4.3:+89209246 | MS.gene34316:CDS |
| AACCCTCAAGACAGATTCAG+CGG | 0.632761 | 4.3:-89209327 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAGTTAGAAATATAAAAA+AGG | - | chr4.3:89209634-89209653 | None:intergenic | 15.0% |
| !!! | TGACAAAAGACTCTTTTTTA+AGG | + | chr4.3:89209364-89209383 | MS.gene34316:CDS | 25.0% |
| AAAGAATGACCTGTAATCAT+TGG | - | chr4.3:89209667-89209686 | None:intergenic | 30.0% | |
| TGAAAAACTGAAATAGACAC+CGG | + | chr4.3:89209428-89209447 | MS.gene34316:intron | 30.0% | |
| ACATCTTCAATCTCAAGTGT+CGG | + | chr4.3:89209551-89209570 | MS.gene34316:intron | 35.0% | |
| ACTCATCTACCAATGATTAC+AGG | + | chr4.3:89209655-89209674 | MS.gene34316:intron | 35.0% | |
| CAAGAGCAGATAATGCATAA+AGG | - | chr4.3:89209691-89209710 | None:intergenic | 35.0% | |
| CTTTATGCATTATCTGCTCT+TGG | + | chr4.3:89209689-89209708 | MS.gene34316:CDS | 35.0% | |
| TTGAGATATTTGCCACTCAA+GGG | - | chr4.3:89209261-89209280 | None:intergenic | 35.0% | |
| ATTATCTGCTCTTGGAAGAC+TGG | + | chr4.3:89209697-89209716 | MS.gene34316:CDS | 40.0% | |
| CTTGAGATATTTGCCACTCA+AGG | - | chr4.3:89209262-89209281 | None:intergenic | 40.0% | |
| GCTTCTTTCGCTAATACTGT+CGG | + | chr4.3:89209302-89209321 | MS.gene34316:CDS | 40.0% | |
| TGAGATATTTGCCACTCAAG+GGG | - | chr4.3:89209260-89209279 | None:intergenic | 40.0% | |
| AACCCTCAAGACAGATTCAG+CGG | - | chr4.3:89209330-89209349 | None:intergenic | 45.0% | |
| ACTGAAATAGACACCGGACA+TGG | + | chr4.3:89209434-89209453 | MS.gene34316:intron | 45.0% | |
| TGTTATGTTCGGCTGACACT+TGG | + | chr4.3:89209578-89209597 | MS.gene34316:intron | 45.0% | |
| ! | GTGTCGGTCAGTGTTATGTT+CGG | + | chr4.3:89209567-89209586 | MS.gene34316:intron | 45.0% |
| !! | CACTTGGTGTTTCGTAAGTG+TGG | + | chr4.3:89209594-89209613 | MS.gene34316:intron | 45.0% |
| TGAGTCGTTGTGTCCATGTC+CGG | - | chr4.3:89209450-89209469 | None:intergenic | 50.0% | |
| TGCCGCTGAATCTGTCTTGA+GGG | + | chr4.3:89209325-89209344 | MS.gene34316:CDS | 50.0% | |
| TTCCTGTTGATCCCCTTGAG+TGG | + | chr4.3:89209246-89209265 | MS.gene34316:CDS | 50.0% | |
| !! | GCTCTTGGAAGACTGGCTTT+GGG | + | chr4.3:89209704-89209723 | MS.gene34316:CDS | 50.0% |
| !! | TGCTCTTGGAAGACTGGCTT+TGG | + | chr4.3:89209703-89209722 | MS.gene34316:CDS | 50.0% |
| GTCTTGAGGGTTGCTGCTAC+TGG | + | chr4.3:89209338-89209357 | MS.gene34316:CDS | 55.0% | |
| TGCCACTCAAGGGGATCAAC+AGG | - | chr4.3:89209251-89209270 | None:intergenic | 55.0% | |
| ! | GTGCCGCTGAATCTGTCTTG+AGG | + | chr4.3:89209324-89209343 | MS.gene34316:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 89209244 | 89209746 | 89209244 | ID=MS.gene34316 |
| chr4.3 | mRNA | 89209244 | 89209746 | 89209244 | ID=MS.gene34316.t1;Parent=MS.gene34316 |
| chr4.3 | exon | 89209244 | 89209385 | 89209244 | ID=MS.gene34316.t1.exon1;Parent=MS.gene34316.t1 |
| chr4.3 | CDS | 89209244 | 89209385 | 89209244 | ID=cds.MS.gene34316.t1;Parent=MS.gene34316.t1 |
| chr4.3 | exon | 89209677 | 89209746 | 89209677 | ID=MS.gene34316.t1.exon2;Parent=MS.gene34316.t1 |
| chr4.3 | CDS | 89209677 | 89209746 | 89209677 | ID=cds.MS.gene34316.t1;Parent=MS.gene34316.t1 |
| Gene Sequence |
| Protein sequence |
