Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052970.t1 | XP_013454489.1 | 100 | 70 | 0 | 0 | 1 | 70 | 72 | 141 | 3.40E-28 | 133.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052970.t1 | Q8GWH5 | 81.4 | 70 | 13 | 0 | 1 | 70 | 57 | 126 | 1.5e-24 | 112.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052970.t1 | A0A072UFI9 | 100.0 | 70 | 0 | 0 | 1 | 70 | 72 | 141 | 2.5e-28 | 133.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050762 | MS.gene052970 | -0.809784 | 1.59E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene052970 | -0.810522 | 1.11E-50 | -1.69E-46 |
| MS.gene052970 | MS.gene055413 | -0.80102 | 1.10E-48 | -1.69E-46 |
| MS.gene052970 | MS.gene060084 | -0.809094 | 2.24E-50 | -1.69E-46 |
| MS.gene052970 | MS.gene063544 | -0.813966 | 1.96E-51 | -1.69E-46 |
| MS.gene052970 | MS.gene071343 | -0.806309 | 8.76E-50 | -1.69E-46 |
| MS.gene052970 | MS.gene072564 | -0.815599 | 8.51E-52 | -1.69E-46 |
| MS.gene052970 | MS.gene072565 | -0.814409 | 1.56E-51 | -1.69E-46 |
| MS.gene052970 | MS.gene21434 | 0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene052970 | MS.gene30792 | 0.801636 | 8.20E-49 | -1.69E-46 |
| MS.gene052970 | MS.gene34314 | 0.947338 | 8.16E-106 | -1.69E-46 |
| MS.gene052970 | MS.gene34316 | 0.972862 | 1.83E-135 | -1.69E-46 |
| MS.gene052970 | MS.gene34317 | 0.947564 | 5.27E-106 | -1.69E-46 |
| MS.gene052970 | MS.gene44540 | -0.813879 | 2.05E-51 | -1.69E-46 |
| MS.gene052970 | MS.gene62623 | -0.804324 | 2.28E-49 | -1.69E-46 |
| MS.gene052970 | MS.gene68877 | -0.840818 | 6.92E-58 | -1.69E-46 |
| MS.gene052970 | MS.gene68933 | -0.825122 | 5.57E-54 | -1.69E-46 |
| MS.gene052970 | MS.gene68959 | -0.828402 | 9.17E-55 | -1.69E-46 |
| MS.gene052970 | MS.gene70819 | -0.820021 | 8.55E-53 | -1.69E-46 |
| MS.gene052970 | MS.gene90199 | -0.803677 | 3.11E-49 | -1.69E-46 |
| MS.gene052970 | MS.gene99154 | -0.823974 | 1.04E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCTTGGAAGACTGGCTT+TGG | 0.289145 | 4.3:+89221155 | MS.gene052970:CDS |
| CTTTATGCATTATCTGCTCT+TGG | 0.374994 | 4.3:+89221141 | MS.gene052970:CDS |
| GTCTTGAGGGTTGCTGCTAC+TGG | 0.407570 | 4.3:+89220790 | MS.gene052970:CDS |
| GCTCTTGGAAGACTGGCTTT+GGG | 0.418794 | 4.3:+89221156 | MS.gene052970:CDS |
| TGCCACTCAAGGGGATCAAC+AGG | 0.434496 | 4.3:-89220700 | None:intergenic |
| TGCCGCTGAATCTGTCTTGA+GGG | 0.443932 | 4.3:+89220777 | MS.gene052970:CDS |
| CAAGAGCAGATAATGCATAA+AGG | 0.448013 | 4.3:-89221140 | None:intergenic |
| ATTATCTGCTCTTGGAAGAC+TGG | 0.464565 | 4.3:+89221149 | MS.gene052970:CDS |
| GGTATTACTATCGCCTATGC+TGG | 0.488512 | 4.3:+89221177 | MS.gene052970:CDS |
| GCTTCTTTCGCTAATACTGT+CGG | 0.494988 | 4.3:+89220754 | MS.gene052970:CDS |
| TTGAGATATTTGCCACTCAA+GGG | 0.504422 | 4.3:-89220710 | None:intergenic |
| CTTGAGATATTTGCCACTCA+AGG | 0.517790 | 4.3:-89220711 | None:intergenic |
| GTGCCGCTGAATCTGTCTTG+AGG | 0.538110 | 4.3:+89220776 | MS.gene052970:CDS |
| AGAAAGGTACGAACCAGCAT+AGG | 0.589035 | 4.3:-89221190 | None:intergenic |
| TGAGATATTTGCCACTCAAG+GGG | 0.608929 | 4.3:-89220709 | None:intergenic |
| TTCCTGTTGATCCCCTTGAG+TGG | 0.613108 | 4.3:+89220698 | MS.gene052970:CDS |
| AACCCTCAAGACAGATTCAG+CGG | 0.632761 | 4.3:-89220779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAGTTAGAAATATAAAAA+AGG | - | chr4.3:89221086-89221105 | None:intergenic | 15.0% |
| !!! | TGACAAAAGACTCTTTTTTA+AGG | + | chr4.3:89220816-89220835 | MS.gene052970:CDS | 25.0% |
| AAAGAATGACCTGTAATCAT+TGG | - | chr4.3:89221119-89221138 | None:intergenic | 30.0% | |
| TGAAAAACTGAAATAGACAC+CGG | + | chr4.3:89220880-89220899 | MS.gene052970:intron | 30.0% | |
| ACATCTTCAATCTCAAGTGT+CGG | + | chr4.3:89221003-89221022 | MS.gene052970:intron | 35.0% | |
| ACTCATCTACCAATGATTAC+AGG | + | chr4.3:89221107-89221126 | MS.gene052970:intron | 35.0% | |
| CAAGAGCAGATAATGCATAA+AGG | - | chr4.3:89221143-89221162 | None:intergenic | 35.0% | |
| CTTTATGCATTATCTGCTCT+TGG | + | chr4.3:89221141-89221160 | MS.gene052970:CDS | 35.0% | |
| TTGAGATATTTGCCACTCAA+GGG | - | chr4.3:89220713-89220732 | None:intergenic | 35.0% | |
| ATTATCTGCTCTTGGAAGAC+TGG | + | chr4.3:89221149-89221168 | MS.gene052970:CDS | 40.0% | |
| CTTGAGATATTTGCCACTCA+AGG | - | chr4.3:89220714-89220733 | None:intergenic | 40.0% | |
| GCTTCTTTCGCTAATACTGT+CGG | + | chr4.3:89220754-89220773 | MS.gene052970:CDS | 40.0% | |
| TGAGATATTTGCCACTCAAG+GGG | - | chr4.3:89220712-89220731 | None:intergenic | 40.0% | |
| AACCCTCAAGACAGATTCAG+CGG | - | chr4.3:89220782-89220801 | None:intergenic | 45.0% | |
| ACTGAAATAGACACCGGACA+TGG | + | chr4.3:89220886-89220905 | MS.gene052970:intron | 45.0% | |
| TGTTATGTTCGGCTGACACT+TGG | + | chr4.3:89221030-89221049 | MS.gene052970:intron | 45.0% | |
| ! | GTGTCGGTCAGTGTTATGTT+CGG | + | chr4.3:89221019-89221038 | MS.gene052970:intron | 45.0% |
| !! | CACTTGGTGTTTCGTAAGTG+TGG | + | chr4.3:89221046-89221065 | MS.gene052970:intron | 45.0% |
| TGAGTCGTTGTGTCCATGTC+CGG | - | chr4.3:89220902-89220921 | None:intergenic | 50.0% | |
| TGCCGCTGAATCTGTCTTGA+GGG | + | chr4.3:89220777-89220796 | MS.gene052970:CDS | 50.0% | |
| TTCCTGTTGATCCCCTTGAG+TGG | + | chr4.3:89220698-89220717 | MS.gene052970:CDS | 50.0% | |
| !! | GCTCTTGGAAGACTGGCTTT+GGG | + | chr4.3:89221156-89221175 | MS.gene052970:CDS | 50.0% |
| !! | TGCTCTTGGAAGACTGGCTT+TGG | + | chr4.3:89221155-89221174 | MS.gene052970:CDS | 50.0% |
| GTCTTGAGGGTTGCTGCTAC+TGG | + | chr4.3:89220790-89220809 | MS.gene052970:CDS | 55.0% | |
| TGCCACTCAAGGGGATCAAC+AGG | - | chr4.3:89220703-89220722 | None:intergenic | 55.0% | |
| ! | GTGCCGCTGAATCTGTCTTG+AGG | + | chr4.3:89220776-89220795 | MS.gene052970:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 89220696 | 89221198 | 89220696 | ID=MS.gene052970 |
| chr4.3 | mRNA | 89220696 | 89221198 | 89220696 | ID=MS.gene052970.t1;Parent=MS.gene052970 |
| chr4.3 | exon | 89220696 | 89220837 | 89220696 | ID=MS.gene052970.t1.exon1;Parent=MS.gene052970.t1 |
| chr4.3 | CDS | 89220696 | 89220837 | 89220696 | ID=cds.MS.gene052970.t1;Parent=MS.gene052970.t1 |
| chr4.3 | exon | 89221129 | 89221198 | 89221129 | ID=MS.gene052970.t1.exon2;Parent=MS.gene052970.t1 |
| chr4.3 | CDS | 89221129 | 89221198 | 89221129 | ID=cds.MS.gene052970.t1;Parent=MS.gene052970.t1 |
| Gene Sequence |
| Protein sequence |
