Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34752.t1 | XP_013445800.1 | 98.8 | 325 | 4 | 0 | 1 | 325 | 1 | 325 | 3.60E-189 | 670.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34752.t1 | P49730 | 83.0 | 329 | 52 | 1 | 1 | 325 | 1 | 329 | 3.9e-160 | 565.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34752.t1 | A0A072TRK2 | 98.8 | 325 | 4 | 0 | 1 | 325 | 1 | 325 | 2.6e-189 | 670.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene34752 | 0.803174 | 3.95E-49 | -1.69E-46 |
| MS.gene050901 | MS.gene34752 | 0.804359 | 2.24E-49 | -1.69E-46 |
| MS.gene05092 | MS.gene34752 | 0.823707 | 1.20E-53 | -1.69E-46 |
| MS.gene05157 | MS.gene34752 | 0.818567 | 1.83E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene34752 | 0.815852 | 7.48E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene34752 | 0.806498 | 7.99E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene34752 | 0.800767 | 1.23E-48 | -1.69E-46 |
| MS.gene054584 | MS.gene34752 | 0.816199 | 6.25E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene34752 | 0.807757 | 4.33E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene34752 | 0.816926 | 4.30E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene34752 | 0.802083 | 6.64E-49 | -1.69E-46 |
| MS.gene056169 | MS.gene34752 | 0.821778 | 3.37E-53 | -1.69E-46 |
| MS.gene060194 | MS.gene34752 | 0.804836 | 1.78E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene34752 | 0.818296 | 2.11E-52 | -1.69E-46 |
| MS.gene060587 | MS.gene34752 | 0.814812 | 1.27E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene34752 | MS.gene034044 | PPI |
| MS.gene34752 | MS.gene034046 | PPI |
| MS.gene026793 | MS.gene34752 | PPI |
| MS.gene79484 | MS.gene34752 | PPI |
| MS.gene37310 | MS.gene34752 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34752.t1 | MTR_8g469010 | 98.769 | 325 | 4 | 0 | 1 | 325 | 1 | 325 | 0.0 | 672 |
| MS.gene34752.t1 | MTR_1g050472 | 93.030 | 330 | 18 | 1 | 1 | 325 | 1 | 330 | 0.0 | 634 |
| MS.gene34752.t1 | MTR_0268s0080 | 91.515 | 330 | 23 | 1 | 1 | 325 | 1 | 330 | 0.0 | 632 |
| MS.gene34752.t1 | MTR_7g029110 | 91.212 | 330 | 24 | 1 | 1 | 325 | 1 | 330 | 0.0 | 610 |
| MS.gene34752.t1 | MTR_4g117370 | 75.926 | 324 | 74 | 1 | 6 | 325 | 18 | 341 | 0.0 | 529 |
| MS.gene34752.t1 | MTR_6g005270 | 69.767 | 43 | 13 | 0 | 265 | 307 | 25 | 67 | 3.99e-14 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34752.t1 | AT3G27060 | 81.325 | 332 | 55 | 3 | 1 | 325 | 1 | 332 | 0.0 | 538 |
| MS.gene34752.t1 | AT3G23580 | 76.543 | 324 | 72 | 1 | 6 | 325 | 18 | 341 | 0.0 | 520 |
Find 55 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGATTTGATTTCGCTTC+AGG | 0.266722 | 8.3:+41274652 | MS.gene34752:CDS |
| CGCTTCCTTGCGCGTTGGTT+GGG | 0.274299 | 8.3:+41274525 | MS.gene34752:CDS |
| GATTTCTTTCATGAATCTTC+CGG | 0.295095 | 8.3:-41274045 | None:intergenic |
| TCAGAAGCGCGTGCGTTCTA+TGG | 0.296534 | 8.3:+41274074 | MS.gene34752:CDS |
| GTTTGTTGCTGATAGGTTAT+TGG | 0.303774 | 8.3:+41274580 | MS.gene34752:CDS |
| GGAGCGAGAAGAGGTTCTTC+TGG | 0.345478 | 8.3:-41273810 | None:intergenic |
| GTTGCTTTCGCTTGTGTTGA+AGG | 0.362449 | 8.3:+41274281 | MS.gene34752:CDS |
| GCGCTTCCTTGCGCGTTGGT+TGG | 0.370769 | 8.3:+41274524 | MS.gene34752:CDS |
| AAGCTGATTGGGCTTTGAAA+TGG | 0.371966 | 8.3:+41274225 | MS.gene34752:CDS |
| GCCGTCGGATGCAGCGAAAA+AGG | 0.376281 | 8.3:-41274003 | None:intergenic |
| TTTGTTGCTGATAGGTTATT+GGG | 0.379609 | 8.3:+41274581 | MS.gene34752:CDS |
| ATGCAGAATCGGTCTGGGTT+TGG | 0.382220 | 8.3:-41273831 | None:intergenic |
| TGCTGATTATTGCTTTACTT+TGG | 0.386701 | 8.3:+41274739 | MS.gene34752:CDS |
| ATGTTCAGAATCCGTTTGAT+TGG | 0.390491 | 8.3:+41274630 | MS.gene34752:CDS |
| TGCGCGTTGGTTGGGATGAA+TGG | 0.417042 | 8.3:+41274533 | MS.gene34752:CDS |
| GGGAACATGCAGAATCGGTC+TGG | 0.418807 | 8.3:-41273837 | None:intergenic |
| GAGCGAGAAGAGGTTCTTCT+GGG | 0.439709 | 8.3:-41273809 | None:intergenic |
| TCAGAATCCGTTTGATTGGA+TGG | 0.443597 | 8.3:+41274634 | MS.gene34752:CDS |
| TATTGCTAAGAAAGCTGATT+GGG | 0.465189 | 8.3:+41274214 | MS.gene34752:CDS |
| AATCGCTTATATACGTTTCG+AGG | 0.468396 | 8.3:-41274145 | None:intergenic |
| ACGCACGCGCTTCTGAGACT+TGG | 0.471778 | 8.3:-41274067 | None:intergenic |
| ATCAAATCCATCCAATCAAA+CGG | 0.489030 | 8.3:-41274641 | None:intergenic |
| GATAGGTTATTGGGTGAACT+TGG | 0.489753 | 8.3:+41274590 | MS.gene34752:CDS |
| GGAACATGCAGAATCGGTCT+GGG | 0.489835 | 8.3:-41273836 | None:intergenic |
| ATATTGAGTTTGTTGCTGAT+AGG | 0.493329 | 8.3:+41274573 | MS.gene34752:CDS |
| GAGCTTATTTCACGTGATGA+AGG | 0.493446 | 8.3:+41274383 | MS.gene34752:CDS |
| ATGGATTTGATTTCGCTTCA+GGG | 0.493555 | 8.3:+41274653 | MS.gene34752:CDS |
| CGACGCGCTTCCTTGCGCGT+TGG | 0.496007 | 8.3:+41274520 | MS.gene34752:CDS |
| GGAGGAAAGATCGACTTCTT+CGG | 0.499480 | 8.3:-41273919 | None:intergenic |
| GGCGGTCCAGAACGAAGCTT+CGG | 0.504261 | 8.3:-41273898 | None:intergenic |
| TTATTGCTTTACTTTGGATG+AGG | 0.510830 | 8.3:+41274745 | MS.gene34752:CDS |
| TTTCCTCCGATCTTCAACAC+TGG | 0.515193 | 8.3:+41273934 | MS.gene34752:CDS |
| GTATTGCTAAGAAAGCTGAT+TGG | 0.515724 | 8.3:+41274213 | MS.gene34752:CDS |
| CAGAAAGCTTCTGTTATGAA+TGG | 0.519026 | 8.3:+41274713 | MS.gene34752:CDS |
| GGATTGGGAACATGCAGAAT+CGG | 0.521690 | 8.3:-41273842 | None:intergenic |
| AGAGGTTCTTCTGGGAAAGA+AGG | 0.536510 | 8.3:-41273801 | None:intergenic |
| TGAAATAAGCTCGTTTGAGA+AGG | 0.538441 | 8.3:-41274372 | None:intergenic |
| GTTGTTCCAGTGTTGAAGAT+CGG | 0.557909 | 8.3:-41273940 | None:intergenic |
| AACGAAGTGGCGTTCGCCGT+CGG | 0.563379 | 8.3:-41273967 | None:intergenic |
| TCTGGGTTTGGAGCGAGAAG+AGG | 0.577657 | 8.3:-41273819 | None:intergenic |
| GTGATGCAGTTGAGATTGAG+AGG | 0.584690 | 8.3:+41274486 | MS.gene34752:CDS |
| TCGTTTGAGAAGGTTAATCC+CGG | 0.587906 | 8.3:-41274362 | None:intergenic |
| TTCATCCCAACCAACGCGCA+AGG | 0.598863 | 8.3:-41274530 | None:intergenic |
| CACTGGAACAACCTCACCGA+CGG | 0.611309 | 8.3:+41273951 | MS.gene34752:CDS |
| ATTGTCCTCGAAAACCTCGC+CGG | 0.613556 | 8.3:+41274026 | MS.gene34752:CDS |
| AGTGGCGTTCGCCGTCGGTG+AGG | 0.618653 | 8.3:-41273962 | None:intergenic |
| TGGATTTGATTTCGCTTCAG+GGG | 0.620413 | 8.3:+41274654 | MS.gene34752:CDS |
| GTTCCAGTGTTGAAGATCGG+AGG | 0.622372 | 8.3:-41273937 | None:intergenic |
| CTTTCATGAATCTTCCGGCG+AGG | 0.631655 | 8.3:-41274040 | None:intergenic |
| CGAGGACATGGGAAACGAAG+TGG | 0.631743 | 8.3:-41273980 | None:intergenic |
| TGATGCAGTTGAGATTGAGA+GGG | 0.641583 | 8.3:+41274487 | MS.gene34752:CDS |
| TCAGCTTTCTTAGCAATACA+CGG | 0.642160 | 8.3:-41274209 | None:intergenic |
| GAAGCTAAGTACAGAGCGTG+TGG | 0.656345 | 8.3:+41274451 | MS.gene34752:CDS |
| AAAGCTTCTGTTATGAATGG+TGG | 0.674687 | 8.3:+41274716 | MS.gene34752:CDS |
| GGAAAGATCGACTTCTTCGG+CGG | 0.687608 | 8.3:-41273916 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCCAATACCCAAAAATCT+GGG | + | chr8.3:41273860-41273879 | MS.gene34752:CDS | 30.0% | |
| ATCAAATCCATCCAATCAAA+CGG | - | chr8.3:41274644-41274663 | None:intergenic | 30.0% | |
| GATTTCTTTCATGAATCTTC+CGG | - | chr8.3:41274048-41274067 | None:intergenic | 30.0% | |
| TATTGCTAAGAAAGCTGATT+GGG | + | chr8.3:41274214-41274233 | MS.gene34752:CDS | 30.0% | |
| TTATTGCTTTACTTTGGATG+AGG | + | chr8.3:41274745-41274764 | MS.gene34752:CDS | 30.0% | |
| TTTGTTGCTGATAGGTTATT+GGG | + | chr8.3:41274581-41274600 | MS.gene34752:CDS | 30.0% | |
| ! | GTTGAAGGAATTTTCTTTTC+AGG | + | chr8.3:41274296-41274315 | MS.gene34752:CDS | 30.0% |
| ! | TGCTGATTATTGCTTTACTT+TGG | + | chr8.3:41274739-41274758 | MS.gene34752:CDS | 30.0% |
| ! | TGTACATTTCCCAGATTTTT+GGG | - | chr8.3:41273872-41273891 | None:intergenic | 30.0% |
| ! | TTGTACATTTCCCAGATTTT+TGG | - | chr8.3:41273873-41273892 | None:intergenic | 30.0% |
| !! | ATATTGAGTTTGTTGCTGAT+AGG | + | chr8.3:41274573-41274592 | MS.gene34752:CDS | 30.0% |
| !!! | AGATTTTTGGGTATTGGATT+GGG | - | chr8.3:41273860-41273879 | None:intergenic | 30.0% |
| !!! | ATTTTCTGGTTGAAAAAACG+CGG | + | chr8.3:41274332-41274351 | MS.gene34752:CDS | 30.0% |
| AAAGCTTCTGTTATGAATGG+TGG | + | chr8.3:41274716-41274735 | MS.gene34752:CDS | 35.0% | |
| AATCGCTTATATACGTTTCG+AGG | - | chr8.3:41274148-41274167 | None:intergenic | 35.0% | |
| ATGTTCAGAATCCGTTTGAT+TGG | + | chr8.3:41274630-41274649 | MS.gene34752:CDS | 35.0% | |
| CAATCCAATACCCAAAAATC+TGG | + | chr8.3:41273859-41273878 | MS.gene34752:CDS | 35.0% | |
| CAGAAAGCTTCTGTTATGAA+TGG | + | chr8.3:41274713-41274732 | MS.gene34752:CDS | 35.0% | |
| GTATTGCTAAGAAAGCTGAT+TGG | + | chr8.3:41274213-41274232 | MS.gene34752:CDS | 35.0% | |
| TCAGCTTTCTTAGCAATACA+CGG | - | chr8.3:41274212-41274231 | None:intergenic | 35.0% | |
| TGAAATAAGCTCGTTTGAGA+AGG | - | chr8.3:41274375-41274394 | None:intergenic | 35.0% | |
| ! | ATGGATTTGATTTCGCTTCA+GGG | + | chr8.3:41274653-41274672 | MS.gene34752:CDS | 35.0% |
| ! | GTTTGTTGCTGATAGGTTAT+TGG | + | chr8.3:41274580-41274599 | MS.gene34752:CDS | 35.0% |
| !!! | AATTTTTTTGAGAAGCGCGT+TGG | + | chr8.3:41274683-41274702 | MS.gene34752:CDS | 35.0% |
| !!! | ATTTTTTTGAGAAGCGCGTT+GGG | + | chr8.3:41274684-41274703 | MS.gene34752:CDS | 35.0% |
| !!! | CAGATTTTTGGGTATTGGAT+TGG | - | chr8.3:41273861-41273880 | None:intergenic | 35.0% |
| !!! | TTTCCCAGATTTTTGGGTAT+TGG | - | chr8.3:41273866-41273885 | None:intergenic | 35.0% |
| AAAAAACGCGGTTTAATGCC+GGG | + | chr8.3:41274344-41274363 | MS.gene34752:CDS | 40.0% | |
| GAAAAAACGCGGTTTAATGC+CGG | + | chr8.3:41274343-41274362 | MS.gene34752:CDS | 40.0% | |
| GAGCTTATTTCACGTGATGA+AGG | + | chr8.3:41274383-41274402 | MS.gene34752:CDS | 40.0% | |
| GATAGGTTATTGGGTGAACT+TGG | + | chr8.3:41274590-41274609 | MS.gene34752:CDS | 40.0% | |
| TCAGAATCCGTTTGATTGGA+TGG | + | chr8.3:41274634-41274653 | MS.gene34752:CDS | 40.0% | |
| TCGTTTGAGAAGGTTAATCC+CGG | - | chr8.3:41274365-41274384 | None:intergenic | 40.0% | |
| TGATGCAGTTGAGATTGAGA+GGG | + | chr8.3:41274487-41274506 | MS.gene34752:CDS | 40.0% | |
| ! | GAATGTTTTCGATAGCGATC+TGG | - | chr8.3:41274103-41274122 | None:intergenic | 40.0% |
| ! | GATGGATTTGATTTCGCTTC+AGG | + | chr8.3:41274652-41274671 | MS.gene34752:CDS | 40.0% |
| ! | GTTGTTCCAGTGTTGAAGAT+CGG | - | chr8.3:41273943-41273962 | None:intergenic | 40.0% |
| ! | TGGATTTGATTTCGCTTCAG+GGG | + | chr8.3:41274654-41274673 | MS.gene34752:CDS | 40.0% |
| !! | AAGCTGATTGGGCTTTGAAA+TGG | + | chr8.3:41274225-41274244 | MS.gene34752:CDS | 40.0% |
| !!! | TTTTTTTGAGAAGCGCGTTG+GGG | + | chr8.3:41274685-41274704 | MS.gene34752:CDS | 40.0% |
| AAAAAGCCGAAGCTTCGTTC+TGG | + | chr8.3:41273892-41273911 | MS.gene34752:CDS | 45.0% | |
| GGATTGGGAACATGCAGAAT+CGG | - | chr8.3:41273845-41273864 | None:intergenic | 45.0% | |
| GTGATGCAGTTGAGATTGAG+AGG | + | chr8.3:41274486-41274505 | MS.gene34752:CDS | 45.0% | |
| TTTCCTCCGATCTTCAACAC+TGG | + | chr8.3:41273934-41273953 | MS.gene34752:CDS | 45.0% | |
| ! | AGAGGTTCTTCTGGGAAAGA+AGG | - | chr8.3:41273804-41273823 | None:intergenic | 45.0% |
| ! | GAAGCTTCTGTGCGATTTTC+TGG | + | chr8.3:41274318-41274337 | MS.gene34752:CDS | 45.0% |
| ! | GGAGGAAAGATCGACTTCTT+CGG | - | chr8.3:41273922-41273941 | None:intergenic | 45.0% |
| ! | GTTGCTTTCGCTTGTGTTGA+AGG | + | chr8.3:41274281-41274300 | MS.gene34752:CDS | 45.0% |
| AGCGAAAAAGGCGAGGACAT+GGG | - | chr8.3:41273994-41274013 | None:intergenic | 50.0% | |
| ATGCAGAATCGGTCTGGGTT+TGG | - | chr8.3:41273834-41273853 | None:intergenic | 50.0% | |
| ATTGTCCTCGAAAACCTCGC+CGG | + | chr8.3:41274026-41274045 | MS.gene34752:CDS | 50.0% | |
| CTTTCATGAATCTTCCGGCG+AGG | - | chr8.3:41274043-41274062 | None:intergenic | 50.0% | |
| GAAGCTAAGTACAGAGCGTG+TGG | + | chr8.3:41274451-41274470 | MS.gene34752:CDS | 50.0% | |
| GAGCGAGAAGAGGTTCTTCT+GGG | - | chr8.3:41273812-41273831 | None:intergenic | 50.0% | |
| GGAACATGCAGAATCGGTCT+GGG | - | chr8.3:41273839-41273858 | None:intergenic | 50.0% | |
| ! | GGAAAGATCGACTTCTTCGG+CGG | - | chr8.3:41273919-41273938 | None:intergenic | 50.0% |
| ! | GTTCCAGTGTTGAAGATCGG+AGG | - | chr8.3:41273940-41273959 | None:intergenic | 50.0% |
| !! | GTTTTCGAGGACAATGCCGT+CGG | - | chr8.3:41274021-41274040 | None:intergenic | 50.0% |
| CACTGGAACAACCTCACCGA+CGG | + | chr8.3:41273951-41273970 | MS.gene34752:CDS | 55.0% | |
| CAGCGAAAAAGGCGAGGACA+TGG | - | chr8.3:41273995-41274014 | None:intergenic | 55.0% | |
| CGAGGACATGGGAAACGAAG+TGG | - | chr8.3:41273983-41274002 | None:intergenic | 55.0% | |
| GGAGCGAGAAGAGGTTCTTC+TGG | - | chr8.3:41273813-41273832 | None:intergenic | 55.0% | |
| GGGAACATGCAGAATCGGTC+TGG | - | chr8.3:41273840-41273859 | None:intergenic | 55.0% | |
| TTCATCCCAACCAACGCGCA+AGG | - | chr8.3:41274533-41274552 | None:intergenic | 55.0% | |
| ! | ATCTTCCGGCGAGGTTTTCG+AGG | - | chr8.3:41274034-41274053 | None:intergenic | 55.0% |
| ! | TCAGAAGCGCGTGCGTTCTA+TGG | + | chr8.3:41274074-41274093 | MS.gene34752:CDS | 55.0% |
| ! | TCTGGGTTTGGAGCGAGAAG+AGG | - | chr8.3:41273822-41273841 | None:intergenic | 55.0% |
| !! | GCCTTTTTCGCTGCATCCGA+CGG | + | chr8.3:41274002-41274021 | MS.gene34752:CDS | 55.0% |
| !! | TGCGCGTTGGTTGGGATGAA+TGG | + | chr8.3:41274533-41274552 | MS.gene34752:CDS | 55.0% |
| AACGAAGTGGCGTTCGCCGT+CGG | - | chr8.3:41273970-41273989 | None:intergenic | 60.0% | |
| CGGATGCAGCGAAAAAGGCG+AGG | - | chr8.3:41274001-41274020 | None:intergenic | 60.0% | |
| GCCGTCGGATGCAGCGAAAA+AGG | - | chr8.3:41274006-41274025 | None:intergenic | 60.0% | |
| GGCGGTCCAGAACGAAGCTT+CGG | - | chr8.3:41273901-41273920 | None:intergenic | 60.0% | |
| !! | ACGCACGCGCTTCTGAGACT+TGG | - | chr8.3:41274070-41274089 | None:intergenic | 60.0% |
| !! | CGCTTCCTTGCGCGTTGGTT+GGG | + | chr8.3:41274525-41274544 | MS.gene34752:CDS | 60.0% |
| ! | GCGCTTCCTTGCGCGTTGGT+TGG | + | chr8.3:41274524-41274543 | MS.gene34752:CDS | 65.0% |
| AGTGGCGTTCGCCGTCGGTG+AGG | - | chr8.3:41273965-41273984 | None:intergenic | 70.0% | |
| CGACGCGCTTCCTTGCGCGT+TGG | + | chr8.3:41274520-41274539 | MS.gene34752:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 41273798 | 41274775 | 41273798 | ID=MS.gene34752 |
| chr8.3 | mRNA | 41273798 | 41274775 | 41273798 | ID=MS.gene34752.t1;Parent=MS.gene34752 |
| chr8.3 | exon | 41273798 | 41274775 | 41273798 | ID=MS.gene34752.t1.exon1;Parent=MS.gene34752.t1 |
| chr8.3 | CDS | 41273798 | 41274775 | 41273798 | ID=cds.MS.gene34752.t1;Parent=MS.gene34752.t1 |
| Gene Sequence |
| Protein sequence |
