AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05092


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05092.t1 MTR_3g063110 98.667 150 2 0 1 150 1 150 1.01e-103 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05092.t1 AT3G46940 87.943 141 17 0 10 150 26 166 5.46e-87 251
MS.gene05092.t1 AT3G46940 87.943 141 17 0 10 150 67 207 6.22e-87 253

Find 46 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGGAGGTTTCTGAGGTTT+TGG 0.233953 3.4:+65321632 MS.gene05092:CDS
GTTAGAGGTGAAGGTGGTTT+TGG 0.293840 3.4:+65321669 MS.gene05092:CDS
GATTTGGATTCTACTGTTAG+AGG 0.343621 3.4:+65321654 MS.gene05092:CDS
TTTCTTCACTCTGAGAAAAC+TGG 0.362791 3.4:-65321204 None:intergenic
ACACGAGCATAGGTTCCTTC+AGG 0.369361 3.4:-65321359 None:intergenic
TCAACAATGTCCAACTTAAA+CGG 0.388632 3.4:+65321170 None:intergenic
CTGATTACAGAGGACCTGTT+GGG 0.415838 3.4:+65321523 MS.gene05092:CDS
CATTCGATTGACGTTGGTGC+TGG 0.432456 3.4:+65321489 MS.gene05092:CDS
TTCCATGTCAACCCCGATCT+TGG 0.442509 3.4:-65321465 None:intergenic
GGCGGAGAGAGGAGAGCCGC+GGG 0.455885 3.4:-65321252 None:intergenic
ATGAGAGCTTTGCCGCGAGC+AGG 0.461473 3.4:-65321311 None:intergenic
TGAAACGACGCCGTTTAAGT+TGG 0.465891 3.4:-65321180 None:intergenic
CGCCAAGATCGGGGTTGACA+TGG 0.476498 3.4:+65321463 MS.gene05092:CDS
TTAAACAGTATAACCCCAAC+AGG 0.497604 3.4:-65321537 None:intergenic
CGCGGCTCTCCTCTCTCCGC+CGG 0.497937 3.4:+65321254 MS.gene05092:CDS
GGAAGGTATAACGGCTTTGT+CGG 0.505146 3.4:-65321231 None:intergenic
GATTCTACTGTTAGAGGTGA+AGG 0.505946 3.4:+65321660 MS.gene05092:CDS
CTCAAGCGCCGCCGATTCAA+AGG 0.511929 3.4:+65321286 MS.gene05092:CDS
CTCTCCATCGCCGTCCCTGA+AGG 0.513836 3.4:+65321344 MS.gene05092:CDS
GGCGCTTGAGAGATCGTATC+CGG 0.519854 3.4:-65321273 None:intergenic
TATTGAGAAGATTATTACTC+CGG 0.523127 3.4:+65321614 MS.gene05092:CDS
CACGAGCATAGGTTCCTTCA+GGG 0.535140 3.4:-65321358 None:intergenic
GCGAGCAGGAACCTTTGAAT+CGG 0.540643 3.4:-65321297 None:intergenic
AGAGCCGCGGGAAGGTATAA+CGG 0.542038 3.4:-65321240 None:intergenic
AGATCGTATCCGGCGGAGAG+AGG 0.550109 3.4:-65321263 None:intergenic
TATTACTCCGGAGGTTTCTG+AGG 0.552838 3.4:+65321626 MS.gene05092:CDS
ACGGCGATGGAGAGATCTGT+GGG 0.554559 3.4:-65321335 None:intergenic
GGTTCCTTCAGGGACGGCGA+TGG 0.559917 3.4:-65321348 None:intergenic
TCCAAAACCTCAGAAACCTC+CGG 0.565876 3.4:-65321633 None:intergenic
TCTACTGTTAGAGGTGAAGG+TGG 0.578300 3.4:+65321663 MS.gene05092:CDS
GACGGCGATGGAGAGATCTG+TGG 0.578622 3.4:-65321336 None:intergenic
AGCAGGAACCTTTGAATCGG+CGG 0.581168 3.4:-65321294 None:intergenic
GTAATTACCAACACGAGCAT+AGG 0.588762 3.4:-65321369 None:intergenic
GAGAGAGGAGAGCCGCGGGA+AGG 0.593764 3.4:-65321248 None:intergenic
GAAGGAACCTATGCTCGTGT+TGG 0.606547 3.4:+65321362 MS.gene05092:CDS
TGAGAAGATTATTACTCCGG+AGG 0.607825 3.4:+65321617 MS.gene05092:CDS
CGGCGGAGAGAGGAGAGCCG+CGG 0.611864 3.4:-65321253 None:intergenic
GCTGATTACAGAGGACCTGT+TGG 0.614644 3.4:+65321522 MS.gene05092:CDS
TGGAAGCATTCGATTGACGT+TGG 0.614719 3.4:+65321483 MS.gene05092:CDS
AGCATAGGTTCCTTCAGGGA+CGG 0.629807 3.4:-65321354 None:intergenic
GATTCAAAGGTTCCTGCTCG+CGG 0.632457 3.4:+65321299 MS.gene05092:CDS
TGATTACAGAGGACCTGTTG+GGG 0.654205 3.4:+65321524 MS.gene05092:CDS
AAAGCCGTTATACCTTCCCG+CGG 0.736048 3.4:+65321236 MS.gene05092:CDS
GTGATTGATGCTGATTACAG+AGG 0.740502 3.4:+65321513 MS.gene05092:CDS
GCTTGAGAGATCGTATCCGG+CGG 0.743244 3.4:-65321270 None:intergenic
CGGCGATGGAGAGATCTGTG+GGG 0.756952 3.4:-65321334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTCAAAAACCTAAAAT+TGG - chr3.4:65321410-65321429 None:intergenic 15.0%
!!! ATTTTAGGTTTTTGAAATTT+AGG + chr3.4:65321410-65321429 MS.gene05092:intron 15.0%
!!! TTTTAGGTTTTTGAAATTTA+GGG + chr3.4:65321411-65321430 MS.gene05092:intron 15.0%
!! AAAAACCTAAAATTGGAAAA+GGG - chr3.4:65321403-65321422 None:intergenic 20.0%
! AAAACCTAAAATTGGAAAAG+GGG - chr3.4:65321402-65321421 None:intergenic 25.0%
! CAAAAACCTAAAATTGGAAA+AGG - chr3.4:65321404-65321423 None:intergenic 25.0%
! TATTGAGAAGATTATTACTC+CGG + chr3.4:65321614-65321633 MS.gene05092:CDS 25.0%
AAACCTAAAATTGGAAAAGG+GGG - chr3.4:65321401-65321420 None:intergenic 30.0%
!!! GTTGATTTTGAAGTGAAAGT+TGG + chr3.4:65321570-65321589 MS.gene05092:CDS 30.0%
GATTTGGATTCTACTGTTAG+AGG + chr3.4:65321654-65321673 MS.gene05092:CDS 35.0%
TTAAACAGTATAACCCCAAC+AGG - chr3.4:65321540-65321559 None:intergenic 35.0%
! TTTCCCCCTTTTCCAATTTT+AGG + chr3.4:65321395-65321414 MS.gene05092:intron 35.0%
! TTTCTTCACTCTGAGAAAAC+TGG - chr3.4:65321207-65321226 None:intergenic 35.0%
GATTCTACTGTTAGAGGTGA+AGG + chr3.4:65321660-65321679 MS.gene05092:CDS 40.0%
GTAATTACCAACACGAGCAT+AGG - chr3.4:65321372-65321391 None:intergenic 40.0%
TGAGAAGATTATTACTCCGG+AGG + chr3.4:65321617-65321636 MS.gene05092:CDS 40.0%
! GTGATTGATGCTGATTACAG+AGG + chr3.4:65321513-65321532 MS.gene05092:CDS 40.0%
!!! AAGGTGGTTTTGGATCTACT+GGG + chr3.4:65321679-65321698 MS.gene05092:CDS 40.0%
!!! GGTTTCTGAGGTTTTGGATT+TGG + chr3.4:65321638-65321657 MS.gene05092:CDS 40.0%
CTGATTACAGAGGACCTGTT+GGG + chr3.4:65321523-65321542 MS.gene05092:CDS 45.0%
TATTACTCCGGAGGTTTCTG+AGG + chr3.4:65321626-65321645 MS.gene05092:CDS 45.0%
TCCAAAACCTCAGAAACCTC+CGG - chr3.4:65321636-65321655 None:intergenic 45.0%
TCTACTGTTAGAGGTGAAGG+TGG + chr3.4:65321663-65321682 MS.gene05092:CDS 45.0%
TGAAACGACGCCGTTTAAGT+TGG - chr3.4:65321183-65321202 None:intergenic 45.0%
TGATTACAGAGGACCTGTTG+GGG + chr3.4:65321524-65321543 MS.gene05092:CDS 45.0%
! ATTTTTGCAGCGCCAAGATC+GGG + chr3.4:65321453-65321472 MS.gene05092:intron 45.0%
! GATTTTTGCAGCGCCAAGAT+CGG + chr3.4:65321452-65321471 MS.gene05092:intron 45.0%
! GGAAGGTATAACGGCTTTGT+CGG - chr3.4:65321234-65321253 None:intergenic 45.0%
! GTTAGAGGTGAAGGTGGTTT+TGG + chr3.4:65321669-65321688 MS.gene05092:CDS 45.0%
!! TGGAAGCATTCGATTGACGT+TGG + chr3.4:65321483-65321502 MS.gene05092:CDS 45.0%
!!! AGGTGGTTTTGGATCTACTG+GGG + chr3.4:65321680-65321699 MS.gene05092:CDS 45.0%
!!! GAAGGTGGTTTTGGATCTAC+TGG + chr3.4:65321678-65321697 MS.gene05092:CDS 45.0%
AAAGCCGTTATACCTTCCCG+CGG + chr3.4:65321236-65321255 MS.gene05092:CDS 50.0%
ACACGAGCATAGGTTCCTTC+AGG - chr3.4:65321362-65321381 None:intergenic 50.0%
AGCAGGAACCTTTGAATCGG+CGG - chr3.4:65321297-65321316 None:intergenic 50.0%
AGCATAGGTTCCTTCAGGGA+CGG - chr3.4:65321357-65321376 None:intergenic 50.0%
CACGAGCATAGGTTCCTTCA+GGG - chr3.4:65321361-65321380 None:intergenic 50.0%
CATTCGATTGACGTTGGTGC+TGG + chr3.4:65321489-65321508 MS.gene05092:CDS 50.0%
GAAGGAACCTATGCTCGTGT+TGG + chr3.4:65321362-65321381 MS.gene05092:CDS 50.0%
GATTCAAAGGTTCCTGCTCG+CGG + chr3.4:65321299-65321318 MS.gene05092:CDS 50.0%
GCGAGCAGGAACCTTTGAAT+CGG - chr3.4:65321300-65321319 None:intergenic 50.0%
GCTGATTACAGAGGACCTGT+TGG + chr3.4:65321522-65321541 MS.gene05092:CDS 50.0%
TTCCATGTCAACCCCGATCT+TGG - chr3.4:65321468-65321487 None:intergenic 50.0%
! TTTTTGCAGCGCCAAGATCG+GGG + chr3.4:65321454-65321473 MS.gene05092:intron 50.0%
!!! TCCGGAGGTTTCTGAGGTTT+TGG + chr3.4:65321632-65321651 MS.gene05092:CDS 50.0%
ACGGCGATGGAGAGATCTGT+GGG - chr3.4:65321338-65321357 None:intergenic 55.0%
GCTTGAGAGATCGTATCCGG+CGG - chr3.4:65321273-65321292 None:intergenic 55.0%
GGCGCTTGAGAGATCGTATC+CGG - chr3.4:65321276-65321295 None:intergenic 55.0%
! AGAGCCGCGGGAAGGTATAA+CGG - chr3.4:65321243-65321262 None:intergenic 55.0%
AGATCGTATCCGGCGGAGAG+AGG - chr3.4:65321266-65321285 None:intergenic 60.0%
ATGAGAGCTTTGCCGCGAGC+AGG - chr3.4:65321314-65321333 None:intergenic 60.0%
CGCCAAGATCGGGGTTGACA+TGG + chr3.4:65321463-65321482 MS.gene05092:CDS 60.0%
CGGCGATGGAGAGATCTGTG+GGG - chr3.4:65321337-65321356 None:intergenic 60.0%
GACGGCGATGGAGAGATCTG+TGG - chr3.4:65321339-65321358 None:intergenic 60.0%
!! CTCAAGCGCCGCCGATTCAA+AGG + chr3.4:65321286-65321305 MS.gene05092:CDS 60.0%
CTCTCCATCGCCGTCCCTGA+AGG + chr3.4:65321344-65321363 MS.gene05092:CDS 65.0%
GGTTCCTTCAGGGACGGCGA+TGG - chr3.4:65321351-65321370 None:intergenic 65.0%
GAGAGAGGAGAGCCGCGGGA+AGG - chr3.4:65321251-65321270 None:intergenic 70.0%
CGCGGCTCTCCTCTCTCCGC+CGG + chr3.4:65321254-65321273 MS.gene05092:CDS 75.0%
CGGCGGAGAGAGGAGAGCCG+CGG - chr3.4:65321256-65321275 None:intergenic 75.0%
GGCGGAGAGAGGAGAGCCGC+GGG - chr3.4:65321255-65321274 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 65321176 65321707 65321176 ID=MS.gene05092
chr3.4 mRNA 65321176 65321707 65321176 ID=MS.gene05092.t1;Parent=MS.gene05092
chr3.4 exon 65321176 65321383 65321176 ID=MS.gene05092.t1.exon1;Parent=MS.gene05092.t1
chr3.4 CDS 65321176 65321383 65321176 ID=cds.MS.gene05092.t1;Parent=MS.gene05092.t1
chr3.4 exon 65321463 65321707 65321463 ID=MS.gene05092.t1.exon2;Parent=MS.gene05092.t1
chr3.4 CDS 65321463 65321707 65321463 ID=cds.MS.gene05092.t1;Parent=MS.gene05092.t1
Gene Sequence

>MS.gene05092

ATGTCCAACTTAAACGGCGTCGTTTCATCCAGTTTTCTCAGAGTGAAGAAACTCTCCGACAAAGCCGTTATACCTTCCCGCGGCTCTCCTCTCTCCGCCGGATACGATCTCTCAAGCGCCGCCGATTCAAAGGTTCCTGCTCGCGGCAAAGCTCTCATCCCCACAGATCTCTCCATCGCCGTCCCTGAAGGAACCTATGCTCGTGTTGGTAATTACTGTTTTCCCCCTTTTCCAATTTTAGGTTTTTGAAATTTAGGGTTTTTGATTGAATTGAATGATTTTTGCAGCGCCAAGATCGGGGTTGACATGGAAGCATTCGATTGACGTTGGTGCTGGAGTGATTGATGCTGATTACAGAGGACCTGTTGGGGTTATACTGTTTAATCATTCTGATGTTGATTTTGAAGTGAAAGTTGGTGATAGAGTTGCTCAGTTGATTATTGAGAAGATTATTACTCCGGAGGTTTCTGAGGTTTTGGATTTGGATTCTACTGTTAGAGGTGAAGGTGGTTTTGGATCTACTGGGGTTTGA

Protein sequence

>MS.gene05092.t1

MSNLNGVVSSSFLRVKKLSDKAVIPSRGSPLSAGYDLSSAADSKVPARGKALIPTDLSIAVPEGTYARVAPRSGLTWKHSIDVGAGVIDADYRGPVGVILFNHSDVDFEVKVGDRVAQLIIEKIITPEVSEVLDLDSTVRGEGGFGSTGV