Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05092.t1 | RHN67997.1 | 98.7 | 150 | 2 | 0 | 1 | 150 | 38 | 187 | 1.00E-74 | 289.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05092.t1 | Q9STG6 | 87.9 | 141 | 17 | 0 | 10 | 150 | 26 | 166 | 1.7e-65 | 250.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05092.t1 | A0A072UXL1 | 98.7 | 150 | 2 | 0 | 1 | 150 | 1 | 150 | 7.5e-75 | 289.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05092 | MS.gene051478 | 0.806326 | 8.69E-50 | -1.69E-46 |
| MS.gene05092 | MS.gene33123 | 0.818799 | 1.62E-52 | -1.69E-46 |
| MS.gene05092 | MS.gene34752 | 0.823707 | 1.20E-53 | -1.69E-46 |
| MS.gene05092 | MS.gene35193 | 0.809867 | 1.53E-50 | -1.69E-46 |
| MS.gene05092 | MS.gene40409 | 0.814625 | 1.40E-51 | -1.69E-46 |
| MS.gene05092 | MS.gene51589 | 0.8173 | 3.54E-52 | -1.69E-46 |
| MS.gene05092 | MS.gene63853 | 0.807975 | 3.89E-50 | -1.69E-46 |
| MS.gene05092 | MS.gene82546 | 0.814109 | 1.82E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene79799 | MS.gene05092 | PPI |
| MS.gene05092 | MS.gene47284 | PPI |
| MS.gene49838 | MS.gene05092 | PPI |
| MS.gene042871 | MS.gene05092 | PPI |
| MS.gene87898 | MS.gene05092 | PPI |
| MS.gene05092 | MS.gene066960 | PPI |
| MS.gene55626 | MS.gene05092 | PPI |
| MS.gene82109 | MS.gene05092 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05092.t1 | MTR_3g063110 | 98.667 | 150 | 2 | 0 | 1 | 150 | 1 | 150 | 1.01e-103 | 293 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05092.t1 | AT3G46940 | 87.943 | 141 | 17 | 0 | 10 | 150 | 26 | 166 | 5.46e-87 | 251 |
| MS.gene05092.t1 | AT3G46940 | 87.943 | 141 | 17 | 0 | 10 | 150 | 67 | 207 | 6.22e-87 | 253 |
Find 46 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCGGAGGTTTCTGAGGTTT+TGG | 0.233953 | 3.4:+65321632 | MS.gene05092:CDS |
| GTTAGAGGTGAAGGTGGTTT+TGG | 0.293840 | 3.4:+65321669 | MS.gene05092:CDS |
| GATTTGGATTCTACTGTTAG+AGG | 0.343621 | 3.4:+65321654 | MS.gene05092:CDS |
| TTTCTTCACTCTGAGAAAAC+TGG | 0.362791 | 3.4:-65321204 | None:intergenic |
| ACACGAGCATAGGTTCCTTC+AGG | 0.369361 | 3.4:-65321359 | None:intergenic |
| TCAACAATGTCCAACTTAAA+CGG | 0.388632 | 3.4:+65321170 | None:intergenic |
| CTGATTACAGAGGACCTGTT+GGG | 0.415838 | 3.4:+65321523 | MS.gene05092:CDS |
| CATTCGATTGACGTTGGTGC+TGG | 0.432456 | 3.4:+65321489 | MS.gene05092:CDS |
| TTCCATGTCAACCCCGATCT+TGG | 0.442509 | 3.4:-65321465 | None:intergenic |
| GGCGGAGAGAGGAGAGCCGC+GGG | 0.455885 | 3.4:-65321252 | None:intergenic |
| ATGAGAGCTTTGCCGCGAGC+AGG | 0.461473 | 3.4:-65321311 | None:intergenic |
| TGAAACGACGCCGTTTAAGT+TGG | 0.465891 | 3.4:-65321180 | None:intergenic |
| CGCCAAGATCGGGGTTGACA+TGG | 0.476498 | 3.4:+65321463 | MS.gene05092:CDS |
| TTAAACAGTATAACCCCAAC+AGG | 0.497604 | 3.4:-65321537 | None:intergenic |
| CGCGGCTCTCCTCTCTCCGC+CGG | 0.497937 | 3.4:+65321254 | MS.gene05092:CDS |
| GGAAGGTATAACGGCTTTGT+CGG | 0.505146 | 3.4:-65321231 | None:intergenic |
| GATTCTACTGTTAGAGGTGA+AGG | 0.505946 | 3.4:+65321660 | MS.gene05092:CDS |
| CTCAAGCGCCGCCGATTCAA+AGG | 0.511929 | 3.4:+65321286 | MS.gene05092:CDS |
| CTCTCCATCGCCGTCCCTGA+AGG | 0.513836 | 3.4:+65321344 | MS.gene05092:CDS |
| GGCGCTTGAGAGATCGTATC+CGG | 0.519854 | 3.4:-65321273 | None:intergenic |
| TATTGAGAAGATTATTACTC+CGG | 0.523127 | 3.4:+65321614 | MS.gene05092:CDS |
| CACGAGCATAGGTTCCTTCA+GGG | 0.535140 | 3.4:-65321358 | None:intergenic |
| GCGAGCAGGAACCTTTGAAT+CGG | 0.540643 | 3.4:-65321297 | None:intergenic |
| AGAGCCGCGGGAAGGTATAA+CGG | 0.542038 | 3.4:-65321240 | None:intergenic |
| AGATCGTATCCGGCGGAGAG+AGG | 0.550109 | 3.4:-65321263 | None:intergenic |
| TATTACTCCGGAGGTTTCTG+AGG | 0.552838 | 3.4:+65321626 | MS.gene05092:CDS |
| ACGGCGATGGAGAGATCTGT+GGG | 0.554559 | 3.4:-65321335 | None:intergenic |
| GGTTCCTTCAGGGACGGCGA+TGG | 0.559917 | 3.4:-65321348 | None:intergenic |
| TCCAAAACCTCAGAAACCTC+CGG | 0.565876 | 3.4:-65321633 | None:intergenic |
| TCTACTGTTAGAGGTGAAGG+TGG | 0.578300 | 3.4:+65321663 | MS.gene05092:CDS |
| GACGGCGATGGAGAGATCTG+TGG | 0.578622 | 3.4:-65321336 | None:intergenic |
| AGCAGGAACCTTTGAATCGG+CGG | 0.581168 | 3.4:-65321294 | None:intergenic |
| GTAATTACCAACACGAGCAT+AGG | 0.588762 | 3.4:-65321369 | None:intergenic |
| GAGAGAGGAGAGCCGCGGGA+AGG | 0.593764 | 3.4:-65321248 | None:intergenic |
| GAAGGAACCTATGCTCGTGT+TGG | 0.606547 | 3.4:+65321362 | MS.gene05092:CDS |
| TGAGAAGATTATTACTCCGG+AGG | 0.607825 | 3.4:+65321617 | MS.gene05092:CDS |
| CGGCGGAGAGAGGAGAGCCG+CGG | 0.611864 | 3.4:-65321253 | None:intergenic |
| GCTGATTACAGAGGACCTGT+TGG | 0.614644 | 3.4:+65321522 | MS.gene05092:CDS |
| TGGAAGCATTCGATTGACGT+TGG | 0.614719 | 3.4:+65321483 | MS.gene05092:CDS |
| AGCATAGGTTCCTTCAGGGA+CGG | 0.629807 | 3.4:-65321354 | None:intergenic |
| GATTCAAAGGTTCCTGCTCG+CGG | 0.632457 | 3.4:+65321299 | MS.gene05092:CDS |
| TGATTACAGAGGACCTGTTG+GGG | 0.654205 | 3.4:+65321524 | MS.gene05092:CDS |
| AAAGCCGTTATACCTTCCCG+CGG | 0.736048 | 3.4:+65321236 | MS.gene05092:CDS |
| GTGATTGATGCTGATTACAG+AGG | 0.740502 | 3.4:+65321513 | MS.gene05092:CDS |
| GCTTGAGAGATCGTATCCGG+CGG | 0.743244 | 3.4:-65321270 | None:intergenic |
| CGGCGATGGAGAGATCTGTG+GGG | 0.756952 | 3.4:-65321334 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTCAAAAACCTAAAAT+TGG | - | chr3.4:65321410-65321429 | None:intergenic | 15.0% |
| !!! | ATTTTAGGTTTTTGAAATTT+AGG | + | chr3.4:65321410-65321429 | MS.gene05092:intron | 15.0% |
| !!! | TTTTAGGTTTTTGAAATTTA+GGG | + | chr3.4:65321411-65321430 | MS.gene05092:intron | 15.0% |
| !! | AAAAACCTAAAATTGGAAAA+GGG | - | chr3.4:65321403-65321422 | None:intergenic | 20.0% |
| ! | AAAACCTAAAATTGGAAAAG+GGG | - | chr3.4:65321402-65321421 | None:intergenic | 25.0% |
| ! | CAAAAACCTAAAATTGGAAA+AGG | - | chr3.4:65321404-65321423 | None:intergenic | 25.0% |
| ! | TATTGAGAAGATTATTACTC+CGG | + | chr3.4:65321614-65321633 | MS.gene05092:CDS | 25.0% |
| AAACCTAAAATTGGAAAAGG+GGG | - | chr3.4:65321401-65321420 | None:intergenic | 30.0% | |
| !!! | GTTGATTTTGAAGTGAAAGT+TGG | + | chr3.4:65321570-65321589 | MS.gene05092:CDS | 30.0% |
| GATTTGGATTCTACTGTTAG+AGG | + | chr3.4:65321654-65321673 | MS.gene05092:CDS | 35.0% | |
| TTAAACAGTATAACCCCAAC+AGG | - | chr3.4:65321540-65321559 | None:intergenic | 35.0% | |
| ! | TTTCCCCCTTTTCCAATTTT+AGG | + | chr3.4:65321395-65321414 | MS.gene05092:intron | 35.0% |
| ! | TTTCTTCACTCTGAGAAAAC+TGG | - | chr3.4:65321207-65321226 | None:intergenic | 35.0% |
| GATTCTACTGTTAGAGGTGA+AGG | + | chr3.4:65321660-65321679 | MS.gene05092:CDS | 40.0% | |
| GTAATTACCAACACGAGCAT+AGG | - | chr3.4:65321372-65321391 | None:intergenic | 40.0% | |
| TGAGAAGATTATTACTCCGG+AGG | + | chr3.4:65321617-65321636 | MS.gene05092:CDS | 40.0% | |
| ! | GTGATTGATGCTGATTACAG+AGG | + | chr3.4:65321513-65321532 | MS.gene05092:CDS | 40.0% |
| !!! | AAGGTGGTTTTGGATCTACT+GGG | + | chr3.4:65321679-65321698 | MS.gene05092:CDS | 40.0% |
| !!! | GGTTTCTGAGGTTTTGGATT+TGG | + | chr3.4:65321638-65321657 | MS.gene05092:CDS | 40.0% |
| CTGATTACAGAGGACCTGTT+GGG | + | chr3.4:65321523-65321542 | MS.gene05092:CDS | 45.0% | |
| TATTACTCCGGAGGTTTCTG+AGG | + | chr3.4:65321626-65321645 | MS.gene05092:CDS | 45.0% | |
| TCCAAAACCTCAGAAACCTC+CGG | - | chr3.4:65321636-65321655 | None:intergenic | 45.0% | |
| TCTACTGTTAGAGGTGAAGG+TGG | + | chr3.4:65321663-65321682 | MS.gene05092:CDS | 45.0% | |
| TGAAACGACGCCGTTTAAGT+TGG | - | chr3.4:65321183-65321202 | None:intergenic | 45.0% | |
| TGATTACAGAGGACCTGTTG+GGG | + | chr3.4:65321524-65321543 | MS.gene05092:CDS | 45.0% | |
| ! | ATTTTTGCAGCGCCAAGATC+GGG | + | chr3.4:65321453-65321472 | MS.gene05092:intron | 45.0% |
| ! | GATTTTTGCAGCGCCAAGAT+CGG | + | chr3.4:65321452-65321471 | MS.gene05092:intron | 45.0% |
| ! | GGAAGGTATAACGGCTTTGT+CGG | - | chr3.4:65321234-65321253 | None:intergenic | 45.0% |
| ! | GTTAGAGGTGAAGGTGGTTT+TGG | + | chr3.4:65321669-65321688 | MS.gene05092:CDS | 45.0% |
| !! | TGGAAGCATTCGATTGACGT+TGG | + | chr3.4:65321483-65321502 | MS.gene05092:CDS | 45.0% |
| !!! | AGGTGGTTTTGGATCTACTG+GGG | + | chr3.4:65321680-65321699 | MS.gene05092:CDS | 45.0% |
| !!! | GAAGGTGGTTTTGGATCTAC+TGG | + | chr3.4:65321678-65321697 | MS.gene05092:CDS | 45.0% |
| AAAGCCGTTATACCTTCCCG+CGG | + | chr3.4:65321236-65321255 | MS.gene05092:CDS | 50.0% | |
| ACACGAGCATAGGTTCCTTC+AGG | - | chr3.4:65321362-65321381 | None:intergenic | 50.0% | |
| AGCAGGAACCTTTGAATCGG+CGG | - | chr3.4:65321297-65321316 | None:intergenic | 50.0% | |
| AGCATAGGTTCCTTCAGGGA+CGG | - | chr3.4:65321357-65321376 | None:intergenic | 50.0% | |
| CACGAGCATAGGTTCCTTCA+GGG | - | chr3.4:65321361-65321380 | None:intergenic | 50.0% | |
| CATTCGATTGACGTTGGTGC+TGG | + | chr3.4:65321489-65321508 | MS.gene05092:CDS | 50.0% | |
| GAAGGAACCTATGCTCGTGT+TGG | + | chr3.4:65321362-65321381 | MS.gene05092:CDS | 50.0% | |
| GATTCAAAGGTTCCTGCTCG+CGG | + | chr3.4:65321299-65321318 | MS.gene05092:CDS | 50.0% | |
| GCGAGCAGGAACCTTTGAAT+CGG | - | chr3.4:65321300-65321319 | None:intergenic | 50.0% | |
| GCTGATTACAGAGGACCTGT+TGG | + | chr3.4:65321522-65321541 | MS.gene05092:CDS | 50.0% | |
| TTCCATGTCAACCCCGATCT+TGG | - | chr3.4:65321468-65321487 | None:intergenic | 50.0% | |
| ! | TTTTTGCAGCGCCAAGATCG+GGG | + | chr3.4:65321454-65321473 | MS.gene05092:intron | 50.0% |
| !!! | TCCGGAGGTTTCTGAGGTTT+TGG | + | chr3.4:65321632-65321651 | MS.gene05092:CDS | 50.0% |
| ACGGCGATGGAGAGATCTGT+GGG | - | chr3.4:65321338-65321357 | None:intergenic | 55.0% | |
| GCTTGAGAGATCGTATCCGG+CGG | - | chr3.4:65321273-65321292 | None:intergenic | 55.0% | |
| GGCGCTTGAGAGATCGTATC+CGG | - | chr3.4:65321276-65321295 | None:intergenic | 55.0% | |
| ! | AGAGCCGCGGGAAGGTATAA+CGG | - | chr3.4:65321243-65321262 | None:intergenic | 55.0% |
| AGATCGTATCCGGCGGAGAG+AGG | - | chr3.4:65321266-65321285 | None:intergenic | 60.0% | |
| ATGAGAGCTTTGCCGCGAGC+AGG | - | chr3.4:65321314-65321333 | None:intergenic | 60.0% | |
| CGCCAAGATCGGGGTTGACA+TGG | + | chr3.4:65321463-65321482 | MS.gene05092:CDS | 60.0% | |
| CGGCGATGGAGAGATCTGTG+GGG | - | chr3.4:65321337-65321356 | None:intergenic | 60.0% | |
| GACGGCGATGGAGAGATCTG+TGG | - | chr3.4:65321339-65321358 | None:intergenic | 60.0% | |
| !! | CTCAAGCGCCGCCGATTCAA+AGG | + | chr3.4:65321286-65321305 | MS.gene05092:CDS | 60.0% |
| CTCTCCATCGCCGTCCCTGA+AGG | + | chr3.4:65321344-65321363 | MS.gene05092:CDS | 65.0% | |
| GGTTCCTTCAGGGACGGCGA+TGG | - | chr3.4:65321351-65321370 | None:intergenic | 65.0% | |
| GAGAGAGGAGAGCCGCGGGA+AGG | - | chr3.4:65321251-65321270 | None:intergenic | 70.0% | |
| CGCGGCTCTCCTCTCTCCGC+CGG | + | chr3.4:65321254-65321273 | MS.gene05092:CDS | 75.0% | |
| CGGCGGAGAGAGGAGAGCCG+CGG | - | chr3.4:65321256-65321275 | None:intergenic | 75.0% | |
| GGCGGAGAGAGGAGAGCCGC+GGG | - | chr3.4:65321255-65321274 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 65321176 | 65321707 | 65321176 | ID=MS.gene05092 |
| chr3.4 | mRNA | 65321176 | 65321707 | 65321176 | ID=MS.gene05092.t1;Parent=MS.gene05092 |
| chr3.4 | exon | 65321176 | 65321383 | 65321176 | ID=MS.gene05092.t1.exon1;Parent=MS.gene05092.t1 |
| chr3.4 | CDS | 65321176 | 65321383 | 65321176 | ID=cds.MS.gene05092.t1;Parent=MS.gene05092.t1 |
| chr3.4 | exon | 65321463 | 65321707 | 65321463 | ID=MS.gene05092.t1.exon2;Parent=MS.gene05092.t1 |
| chr3.4 | CDS | 65321463 | 65321707 | 65321463 | ID=cds.MS.gene05092.t1;Parent=MS.gene05092.t1 |
| Gene Sequence |
| Protein sequence |