AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34829

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34829.t1 MTR_2g060350 95.721 444 19 0 1 444 1 444 0.0 898
MS.gene34829.t1 MTR_3g030540 59.775 445 169 6 1 443 1 437 0.0 521
MS.gene34829.t1 MTR_1g052315 52.392 439 193 9 10 441 1 430 1.07e-149 433
MS.gene34829.t1 MTR_7g091850 50.339 443 204 7 11 444 11 446 1.62e-137 402
MS.gene34829.t1 MTR_7g091840 53.799 329 144 5 62 385 53 378 4.19e-118 350
MS.gene34829.t1 MTR_2g103410 46.092 371 193 5 73 439 58 425 7.60e-113 339
MS.gene34829.t1 MTR_8g065030 38.914 442 243 9 7 439 6 429 1.52e-108 328
MS.gene34829.t1 MTR_6g005630 43.732 343 180 7 76 415 47 379 2.74e-92 286
MS.gene34829.t1 MTR_4g094715 43.251 363 191 7 68 419 59 417 4.97e-90 281
MS.gene34829.t1 MTR_7g110050 40.872 367 207 6 62 419 55 420 1.01e-89 280
MS.gene34829.t1 MTR_3g084360 40.108 369 202 7 70 430 20 377 1.16e-86 270
MS.gene34829.t1 MTR_2g049400 41.623 382 205 10 65 440 19 388 2.41e-86 269
MS.gene34829.t1 MTR_5g033680 38.043 368 219 7 79 439 64 429 3.65e-86 271
MS.gene34829.t1 MTR_1g086390 40.872 367 192 9 70 419 77 435 4.98e-86 271
MS.gene34829.t1 MTR_8g065010 38.360 378 196 7 70 416 103 474 7.82e-84 267
MS.gene34829.t1 MTR_4g084050 37.867 375 205 8 71 430 20 381 1.45e-83 262
MS.gene34829.t1 MTR_5g043350 40.833 360 195 8 79 430 34 383 1.02e-82 260
MS.gene34829.t1 MTR_3g084380 39.496 357 195 7 79 426 26 370 3.49e-81 256
MS.gene34829.t1 MTR_7g113910 49.042 261 112 3 166 426 95 334 4.38e-80 253
MS.gene34829.t1 MTR_2g103650 40.233 343 197 5 76 412 30 370 1.45e-79 252
MS.gene34829.t1 MTR_5g034090 38.275 371 193 8 76 416 123 487 1.54e-79 256
MS.gene34829.t1 MTR_3g054320 37.778 360 197 10 79 428 35 377 1.60e-79 252
MS.gene34829.t1 MTR_6g005630 41.399 343 165 10 76 415 47 356 2.90e-79 251
MS.gene34829.t1 MTR_6g028080 38.420 367 206 8 75 432 31 386 3.13e-78 248
MS.gene34829.t1 MTR_4g120320 37.469 403 230 14 51 443 32 422 8.89e-77 245
MS.gene34829.t1 MTR_3g438050 41.209 364 192 12 68 419 17 370 2.14e-76 244
MS.gene34829.t1 MTR_2g435640 35.641 390 226 12 62 439 43 419 1.05e-75 243
MS.gene34829.t1 MTR_2g032710 39.669 363 204 7 68 419 61 419 3.95e-75 243
MS.gene34829.t1 MTR_8g010080 39.084 371 200 9 70 434 27 377 1.75e-74 238
MS.gene34829.t1 MTR_8g030930 39.718 355 197 9 73 418 26 372 6.12e-74 237
MS.gene34829.t1 MTR_8g010110 38.859 368 200 9 76 437 33 381 6.80e-74 237
MS.gene34829.t1 MTR_8g010070 38.281 384 206 10 58 434 20 379 1.11e-73 236
MS.gene34829.t1 MTR_8g010090 38.587 368 201 9 76 437 33 381 4.42e-73 235
MS.gene34829.t1 MTR_2g103410 45.247 263 137 5 73 331 58 317 1.95e-72 231
MS.gene34829.t1 MTR_4g083660 39.437 355 200 10 66 414 1 346 3.45e-72 232
MS.gene34829.t1 MTR_8g009810 38.968 349 200 8 74 417 35 375 2.86e-71 231
MS.gene34829.t1 MTR_0157s0060 36.842 361 205 6 78 430 2 347 8.72e-69 223
MS.gene34829.t1 MTR_4g034020 36.842 361 205 6 78 430 2 347 8.72e-69 223
MS.gene34829.t1 MTR_2g058670 34.908 381 237 9 66 441 17 391 4.36e-68 223
MS.gene34829.t1 MTR_8g008765 37.092 337 189 6 70 401 27 345 1.50e-67 219
MS.gene34829.t1 MTR_7g035415 40.625 288 151 6 80 360 46 320 1.79e-67 218
MS.gene34829.t1 MTR_2g058840 34.908 381 235 10 66 441 17 389 1.85e-67 221
MS.gene34829.t1 MTR_2g029170 37.537 341 198 9 104 440 29 358 1.30e-64 212
MS.gene34829.t1 MTR_2g058640 36.053 380 220 9 73 441 24 391 3.04e-64 213
MS.gene34829.t1 MTR_8g010040 35.933 359 204 9 76 428 34 372 2.63e-63 209
MS.gene34829.t1 MTR_1g088160 32.911 395 243 11 57 439 18 402 4.22e-63 209
MS.gene34829.t1 MTR_1g012390 34.677 372 226 9 66 430 20 381 2.28e-61 204
MS.gene34829.t1 MTR_5g097860 35.494 324 196 8 73 390 24 340 4.02e-58 194
MS.gene34829.t1 MTR_1g013740 33.140 344 202 7 74 412 35 355 6.89e-58 195
MS.gene34829.t1 MTR_8g077320 37.500 304 141 6 73 360 32 302 1.73e-57 193
MS.gene34829.t1 MTR_5g063430 36.213 301 174 8 73 367 24 312 4.59e-57 191
MS.gene34829.t1 MTR_1g013730 34.302 344 198 9 74 412 27 347 5.99e-57 192
MS.gene34829.t1 MTR_5g034090 37.762 286 144 6 76 331 123 404 1.30e-56 192
MS.gene34829.t1 MTR_8g010050 32.990 388 200 9 54 434 11 345 1.43e-56 191
MS.gene34829.t1 MTR_8g008700 38.148 270 147 4 131 398 14 265 5.14e-54 182
MS.gene34829.t1 MTR_8g031180 39.269 219 123 3 201 414 59 272 1.41e-45 159
MS.gene34829.t1 MTR_2g100040 32.295 353 179 10 67 412 17 316 3.62e-43 155
MS.gene34829.t1 MTR_4g120730 24.198 405 230 11 74 429 40 416 1.62e-21 97.1
MS.gene34829.t1 MTR_2g042740 28.958 259 159 7 168 412 169 416 2.11e-20 93.6
MS.gene34829.t1 MTR_3g449590 25.581 387 230 14 74 421 47 414 5.53e-20 92.4
MS.gene34829.t1 MTR_5g090160 27.273 264 169 6 168 419 158 410 3.01e-18 87.0
MS.gene34829.t1 MTR_7g110710 26.158 367 201 13 74 394 43 385 3.68e-18 87.0
MS.gene34829.t1 MTR_4g124980 26.255 259 166 7 168 412 187 434 4.26e-18 87.0
MS.gene34829.t1 MTR_7g110710 26.158 367 201 13 74 394 43 385 4.30e-18 86.7
MS.gene34829.t1 MTR_1g086030 26.891 357 194 13 74 386 82 415 5.18e-18 86.7
MS.gene34829.t1 MTR_1g086030 29.870 231 140 5 168 386 91 311 1.17e-17 85.1
MS.gene34829.t1 MTR_2g435340 21.839 348 223 7 119 429 13 348 3.27e-17 83.6
MS.gene34829.t1 MTR_2g435340 21.975 405 239 10 74 429 37 413 5.42e-16 80.1
MS.gene34829.t1 MTR_7g088550 23.077 403 250 15 54 415 30 413 8.24e-16 79.7
MS.gene34829.t1 MTR_3g096330 25.794 252 163 6 157 394 177 418 1.24e-15 79.7
MS.gene34829.t1 MTR_8g098840 25.633 316 177 11 75 350 45 342 2.08e-15 78.6
MS.gene34829.t1 MTR_8g028725 24.148 352 219 13 74 390 55 393 6.38e-15 77.0
MS.gene34829.t1 MTR_1g100060 22.487 378 238 11 76 415 95 455 7.12e-14 73.9
MS.gene34829.t1 MTR_7g078680 22.573 412 251 13 75 441 56 444 9.18e-14 73.6
MS.gene34829.t1 MTR_1g100050 22.222 378 239 12 76 415 52 412 2.99e-12 68.6
MS.gene34829.t1 MTR_7g092470 24.444 270 178 5 147 400 123 382 7.42e-12 67.4
MS.gene34829.t1 MTR_1g099850 22.642 371 246 14 76 415 57 417 3.94e-11 65.1
MS.gene34829.t1 MTR_1g100040 22.102 371 248 13 76 415 58 418 4.32e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34829.t1 AT3G07970 52.423 454 189 11 1 442 1 439 3.49e-150 434
MS.gene34829.t1 AT3G57510 48.416 442 200 12 11 442 8 431 9.75e-138 402
MS.gene34829.t1 AT2G41850 49.433 441 197 12 10 441 7 430 8.47e-127 374
MS.gene34829.t1 AT1G80170 47.606 376 189 6 69 439 48 420 2.91e-117 350
MS.gene34829.t1 AT1G23460 45.119 379 199 7 66 439 33 407 2.79e-108 327
MS.gene34829.t1 AT1G23460 45.119 379 199 7 66 439 57 431 5.02e-108 327
MS.gene34829.t1 AT1G70500 43.536 379 205 7 66 439 57 431 1.87e-104 318
MS.gene34829.t1 AT1G56710 43.697 357 184 7 76 419 50 402 3.55e-92 286
MS.gene34829.t1 AT3G15720 42.703 370 190 8 70 430 19 375 4.69e-92 286
MS.gene34829.t1 AT3G15720 42.703 370 190 8 70 430 20 376 5.10e-92 286
MS.gene34829.t1 AT5G14650 40.710 366 202 7 59 419 36 391 6.68e-90 280
MS.gene34829.t1 AT3G15720 42.432 370 188 9 70 430 19 372 4.37e-89 278
MS.gene34829.t1 AT1G48100 40.054 367 197 8 68 419 74 432 1.72e-87 275
MS.gene34829.t1 AT2G43890 39.257 377 217 8 67 440 22 389 2.96e-86 269
MS.gene34829.t1 AT4G35670 41.111 360 195 7 76 429 26 374 5.43e-86 268
MS.gene34829.t1 AT3G26610 35.714 420 250 9 6 419 8 413 3.78e-85 269
MS.gene34829.t1 AT5G27530 39.665 358 191 5 79 430 58 396 2.75e-84 266
MS.gene34829.t1 AT1G05650 39.362 376 217 7 67 439 21 388 1.41e-83 262
MS.gene34829.t1 AT3G15720 43.034 323 165 6 114 430 3 312 1.04e-82 260
MS.gene34829.t1 AT1G02460 41.033 368 189 9 72 419 87 446 1.05e-82 263
MS.gene34829.t1 AT1G05660 38.992 377 217 8 67 439 21 388 8.36e-82 258
MS.gene34829.t1 AT1G60590 38.378 370 197 7 76 416 125 492 1.10e-81 262
MS.gene34829.t1 AT1G10640 38.147 367 199 7 76 416 121 485 1.41e-80 259
MS.gene34829.t1 AT1G80140 41.195 318 169 7 108 419 6 311 3.44e-80 251
MS.gene34829.t1 AT4G18180 37.566 378 210 8 73 430 38 409 3.00e-78 249
MS.gene34829.t1 AT5G44840 37.500 360 209 7 76 429 26 375 1.13e-77 247
MS.gene34829.t1 AT1G80170 46.816 267 135 5 69 331 48 311 2.29e-77 244
MS.gene34829.t1 AT2G43870 39.164 383 216 12 65 441 12 383 5.93e-76 242
MS.gene34829.t1 AT2G33160 39.096 376 213 9 73 439 31 399 8.29e-76 249
MS.gene34829.t1 AT5G17200 38.760 387 196 9 70 429 35 407 9.62e-76 243
MS.gene34829.t1 AT4G01890 38.268 358 201 8 76 417 67 420 1.28e-75 244
MS.gene34829.t1 AT3G59850 40.058 342 190 10 79 414 28 360 2.64e-74 238
MS.gene34829.t1 AT2G15460 35.884 379 224 9 73 440 30 400 2.75e-74 238
MS.gene34829.t1 AT1G43080 37.368 380 217 11 73 440 30 400 3.49e-74 238
MS.gene34829.t1 AT3G59850 39.942 343 191 10 78 414 71 404 4.80e-74 239
MS.gene34829.t1 AT2G15450 35.714 378 224 9 73 439 30 399 1.36e-73 237
MS.gene34829.t1 AT1G02790 37.337 383 207 11 76 441 54 420 1.83e-73 237
MS.gene34829.t1 AT2G15470 35.620 379 225 9 73 440 30 400 5.80e-73 235
MS.gene34829.t1 AT1G43090 36.800 375 218 9 73 436 30 396 1.58e-72 235
MS.gene34829.t1 AT2G26620 36.340 377 223 9 73 440 30 398 5.33e-72 233
MS.gene34829.t1 AT2G43880 38.810 353 201 8 67 414 24 366 1.83e-71 231
MS.gene34829.t1 AT2G40310 36.340 377 219 11 76 440 33 400 2.85e-71 231
MS.gene34829.t1 AT1G43100 36.533 375 219 9 73 436 30 396 2.86e-71 232
MS.gene34829.t1 AT1G17150 37.466 371 217 9 76 439 35 397 3.10e-71 231
MS.gene34829.t1 AT1G65570 35.162 401 234 10 46 440 14 394 4.06e-71 230
MS.gene34829.t1 AT4G35670 42.238 277 149 2 153 429 2 267 9.06e-71 226
MS.gene34829.t1 AT4G13760 35.092 379 227 9 73 440 30 400 1.52e-70 229
MS.gene34829.t1 AT3G07820 36.314 369 219 9 81 442 28 387 1.74e-70 228
MS.gene34829.t1 AT1G78400 36.828 372 218 11 76 439 37 399 1.99e-70 228
MS.gene34829.t1 AT2G43860 38.997 359 203 10 64 414 20 370 2.64e-70 228
MS.gene34829.t1 AT5G48140 37.607 351 203 8 85 429 33 373 7.77e-70 227
MS.gene34829.t1 AT3G07840 35.580 371 216 9 73 429 13 374 3.86e-69 225
MS.gene34829.t1 AT3G07830 35.359 362 219 8 85 440 33 385 5.19e-69 224
MS.gene34829.t1 AT3G14040 36.997 373 219 9 74 439 71 434 1.64e-67 222
MS.gene34829.t1 AT3G07850 36.675 379 224 9 68 439 64 433 4.91e-67 221
MS.gene34829.t1 AT5G44830 36.503 326 185 4 106 429 11 316 6.06e-67 217
MS.gene34829.t1 AT4G32380 34.677 372 223 8 73 434 25 386 2.10e-66 219
MS.gene34829.t1 AT5G14650 38.489 278 158 5 59 331 36 305 4.14e-61 201
MS.gene34829.t1 AT4G32370 37.540 309 171 7 69 365 29 327 7.50e-61 202
MS.gene34829.t1 AT2G43880 38.511 309 175 8 67 370 24 322 3.22e-58 194
MS.gene34829.t1 AT4G32375 39.080 261 148 3 153 412 13 263 3.80e-58 197
MS.gene34829.t1 AT5G39910 34.426 305 169 7 73 369 35 316 2.13e-51 178
MS.gene34829.t1 AT4G32380 30.811 370 199 8 73 434 25 345 2.21e-51 179
MS.gene34829.t1 AT1G19170 28.185 259 161 6 168 412 201 448 6.77e-21 95.5
MS.gene34829.t1 AT3G62110 28.879 232 142 5 168 386 156 377 1.19e-20 94.7
MS.gene34829.t1 AT3G62110 28.879 232 142 5 168 386 156 377 1.19e-20 94.7
MS.gene34829.t1 AT3G42950 26.738 374 228 14 73 412 65 426 3.26e-20 93.2
MS.gene34829.t1 AT4G23820 24.557 395 240 14 74 429 41 416 2.57e-19 90.5
MS.gene34829.t1 AT4G33440 25.000 392 218 14 73 408 71 442 7.42e-18 86.3
MS.gene34829.t1 AT3G06770 24.147 381 226 12 74 412 43 402 4.07e-17 83.6
MS.gene34829.t1 AT3G16850 23.774 265 170 5 168 415 153 402 4.24e-17 83.6
MS.gene34829.t1 AT3G06770 24.147 381 226 12 74 412 44 403 4.40e-17 83.6
MS.gene34829.t1 AT2G23900 25.000 316 178 10 76 350 58 355 6.28e-17 83.2
MS.gene34829.t1 AT5G41870 24.859 354 208 14 73 386 44 379 1.03e-16 82.4
MS.gene34829.t1 AT3G48950 23.919 347 199 11 47 350 20 344 1.69e-16 82.0
MS.gene34829.t1 AT5G49215 23.438 384 231 11 74 415 41 403 1.88e-16 81.6
MS.gene34829.t1 AT5G49215 23.438 384 231 11 74 415 43 405 1.95e-16 81.6
MS.gene34829.t1 AT4G23500 25.316 316 178 11 75 350 69 366 1.01e-15 79.7
MS.gene34829.t1 AT3G06770 24.031 258 172 5 168 412 87 333 4.28e-15 77.0
MS.gene34829.t1 AT3G06770 24.031 258 172 5 168 412 87 333 4.28e-15 77.0
MS.gene34829.t1 AT3G06770 24.031 258 172 5 168 412 69 315 4.83e-15 76.6
MS.gene34829.t1 AT3G61490 30.208 192 113 6 168 346 271 454 7.26e-14 73.9
MS.gene34829.t1 AT3G61490 30.208 192 113 6 168 346 173 356 7.44e-14 73.9
MS.gene34829.t1 AT3G61490 30.366 191 112 6 168 345 158 340 8.48e-14 73.6
MS.gene34829.t1 AT3G61490 30.366 191 112 6 168 345 158 340 8.48e-14 73.6
MS.gene34829.t1 AT3G61490 30.366 191 112 6 168 345 158 340 8.48e-14 73.6
MS.gene34829.t1 AT3G57790 28.037 214 139 5 74 272 35 248 4.69e-11 65.1
MS.gene34829.t1 AT3G57790 28.037 214 139 5 74 272 35 248 4.69e-11 65.1

Find 89 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCAAATACTGCCTTTA+TGG 0.149151 4.4:-61178858 MS.gene34829:intron
GACATAATAAACTGTGATAT+TGG 0.217261 4.4:-61177819 MS.gene34829:CDS
CTCACCATAAAGGCAGTATT+TGG 0.245870 4.4:+61178854 None:intergenic
AAAACAAAACATCATCATTT+TGG 0.257008 4.4:-61179210 MS.gene34829:CDS
AATTCATCATGTTAGCTCTA+TGG 0.292346 4.4:-61178516 MS.gene34829:intron
GTAGTTCCTCCACCACCTTC+TGG 0.295016 4.4:+61176093 None:intergenic
TTCTGCAGTATTGGAAGTTT+AGG 0.326431 4.4:-61176949 MS.gene34829:intron
GTTATTGTTGGAATATTTGC+AGG 0.329378 4.4:+61178356 None:intergenic
ATTTGAGAGTAAAGAATCTA+AGG 0.331559 4.4:-61177974 MS.gene34829:CDS
GAGATGGTATATCCTATTCT+TGG 0.332828 4.4:+61178907 None:intergenic
TTGCAGAGAAATATACTTGC+AGG 0.334080 4.4:-61176130 MS.gene34829:CDS
GCACGGCTTTGTTCCGCTCT+TGG 0.338883 4.4:+61176238 None:intergenic
GTAGTATTGATCTATGATTA+TGG 0.353266 4.4:+61176281 None:intergenic
ATCATGAGAAGTGTGATAAT+TGG 0.354858 4.4:+61179336 None:intergenic
GTGAACAGGGCTGCCTTAAC+AGG 0.368388 4.4:-61176889 MS.gene34829:CDS
GTCTCAAATTTCAAAGTTGA+TGG 0.380238 4.4:-61178423 MS.gene34829:CDS
GCTCTTGGCATGGCTCTGTT+TGG 0.383793 4.4:+61176253 None:intergenic
GCGGACCCGGACATGGAATC+AGG 0.384459 4.4:-61177609 MS.gene34829:intron
AAAACTTACAAGGTTATTGT+TGG 0.389616 4.4:+61178344 None:intergenic
TCCATTAGTTGTTCCTGTTA+AGG 0.393409 4.4:+61176876 None:intergenic
ACATAATAAACTGTGATATT+GGG 0.395546 4.4:-61177818 MS.gene34829:CDS
TTTCCAATGTAGTAGTGAAC+AGG 0.405039 4.4:-61176903 MS.gene34829:CDS
CTCCTTTAAGGCATTTAAGA+AGG 0.412653 4.4:-61178972 MS.gene34829:intron
ATATGATCACAGGGTGGTTC+AGG 0.430537 4.4:-61176357 MS.gene34829:intron
ATGAAACCTGATTCCATGTC+CGG 0.431003 4.4:+61177603 None:intergenic
GCCTTAACAGGAACAACTAA+TGG 0.431469 4.4:-61176877 MS.gene34829:CDS
TGTCAGCATATGAAGAAGAT+AGG 0.435363 4.4:-61178470 MS.gene34829:CDS
TATGATCTTACAGTTGGTCT+TGG 0.435649 4.4:+61178101 None:intergenic
GATTGTATTTCGATAGTTAG+TGG 0.441436 4.4:-61177664 MS.gene34829:CDS
TAGTATTGATCTATGATTAT+GGG 0.445220 4.4:+61176282 None:intergenic
TCCTCAATGCTCTCCACAAT+AGG 0.452988 4.4:-61176025 None:intergenic
GATGGTGGTGGCACTTTCAA+TGG 0.457262 4.4:-61178405 MS.gene34829:CDS
AGATGGTATATCCTATTCTT+GGG 0.459795 4.4:+61178908 None:intergenic
TAGTGTAAGACTCACCATAA+AGG 0.459802 4.4:+61178844 None:intergenic
CACATGAATTCCATCGGTGT+TGG 0.469737 4.4:+61177860 None:intergenic
GCTTTGTTCCGCTCTTGGCA+TGG 0.478055 4.4:+61176243 None:intergenic
CAAAACTTGGCTTAATTGCA+CGG 0.479353 4.4:+61176221 None:intergenic
ACATGAATTCCATCGGTGTT+GGG 0.481545 4.4:+61177861 None:intergenic
ACAATATCCATTGCCTATTG+TGG 0.484295 4.4:+61176012 None:intergenic
TGGAATGAAGCATGTTCTAA+AGG 0.492280 4.4:-61178947 MS.gene34829:CDS
AGCCCTGTTCACTACTACAT+TGG 0.494296 4.4:+61176900 None:intergenic
CTCTTCAAAGTCTCAAGGAA+TGG 0.495111 4.4:-61179154 MS.gene34829:CDS
GGAATGAAGCATGTTCTAAA+GGG 0.497809 4.4:-61178946 MS.gene34829:CDS
ATGGTTAGTGTTGATGACTT+TGG 0.500293 4.4:-61179135 MS.gene34829:CDS
AGATGGAAGAGATGATAGAG+AGG 0.500756 4.4:-61179103 MS.gene34829:intron
GACTTTGGAGCAAGAGCAGA+TGG 0.502754 4.4:-61179120 MS.gene34829:CDS
TCCTTGTGGTGCCCAAGAAT+AGG 0.505871 4.4:-61178919 MS.gene34829:CDS
TCGGTGTTGGGACTATCCTC+TGG 0.506883 4.4:+61177873 None:intergenic
AATTTCAAAGTTGATGGTGG+TGG 0.508006 4.4:-61178417 MS.gene34829:CDS
TTTCTCTGCAAGGAACGCTT+CGG 0.508888 4.4:+61176142 None:intergenic
TTAAGGCATTTAAGAAGGCA+TGG 0.511868 4.4:-61178967 MS.gene34829:intron
ATAGATATCACTTGCGGACC+CGG 0.513667 4.4:-61177622 MS.gene34829:CDS
TCAACATTGGCTTGATGAAG+TGG 0.522002 4.4:+61179267 None:intergenic
GTGGCACTTTCAATGGCAAT+GGG 0.535626 4.4:-61178398 MS.gene34829:CDS
ATCACTTGCGGACCCGGACA+TGG 0.542140 4.4:-61177616 MS.gene34829:CDS
GGTGGCACTTTCAATGGCAA+TGG 0.545089 4.4:-61178399 MS.gene34829:CDS
GGTCTTGGTTCATCATTGCA+TGG 0.546282 4.4:+61178116 None:intergenic
ATGCAATGGCTCTTACATAG+TGG 0.549275 4.4:-61179292 MS.gene34829:CDS
ATGAAGAAGATAGGCAACAC+TGG 0.549453 4.4:-61178461 MS.gene34829:CDS
ATAAACTGTGATATTGGGAC+AGG 0.552284 4.4:-61177813 MS.gene34829:intron
TCAATCTACCATCATGTACA+TGG 0.568484 4.4:+61179235 None:intergenic
AATGTTAGATATGTAAATAG+AGG 0.571277 4.4:-61176057 MS.gene34829:CDS
TTCAGCCACATGAATTCCAT+CGG 0.571554 4.4:+61177854 None:intergenic
TTCTAAAGGGTCTATCCTTG+TGG 0.572564 4.4:-61178933 MS.gene34829:CDS
TGAAACCTGATTCCATGTCC+GGG 0.576277 4.4:+61177604 None:intergenic
CTAGAACCAGAAGGTGGTGG+AGG 0.582001 4.4:-61176099 MS.gene34829:CDS
GCCTATTGTGGAGAGCATTG+AGG 0.588158 4.4:+61176024 None:intergenic
TGCAAGTATATTTCTCTGCA+AGG 0.588447 4.4:+61176132 None:intergenic
GAACATAGAAATGCAAAATG+TGG 0.591101 4.4:-61176310 MS.gene34829:CDS
ATTCTAGAACCAGAAGGTGG+TGG 0.593437 4.4:-61176102 MS.gene34829:CDS
AAGGCAGTATTTGGTTGACA+TGG 0.594353 4.4:+61178863 None:intergenic
AATGCTACATGCATTTGTTG+TGG 0.595049 4.4:+61177942 None:intergenic
TGCATATGAGTAATGAAGCT+AGG 0.596335 4.4:+61179310 None:intergenic
GAGGATAGTCCCAACACCGA+TGG 0.596952 4.4:-61177870 MS.gene34829:CDS
CGTTACTACCATGTACATGA+TGG 0.600317 4.4:-61179243 MS.gene34829:CDS
TCCTATTCTTGGGCACCACA+AGG 0.600648 4.4:+61178918 None:intergenic
TTAGCTCTATGGAACAATTG+AGG 0.602214 4.4:-61178505 MS.gene34829:intron
ATGGTAGTAACGTTCAACAT+TGG 0.605356 4.4:+61179254 None:intergenic
TTTGATTGTGAGAGCGCCAG+AGG 0.607314 4.4:-61177889 MS.gene34829:CDS
AAACAGAGCCATGCCAAGAG+CGG 0.620744 4.4:-61176251 MS.gene34829:CDS
GCTTCATTACTCATATGCAA+TGG 0.623445 4.4:-61179306 MS.gene34829:CDS
TCAAATTTCAAAGTTGATGG+TGG 0.632469 4.4:-61178420 MS.gene34829:CDS
TCTATGGAACAATTGAGGCA+TGG 0.634765 4.4:-61178500 MS.gene34829:CDS
TGAAGAAGATAGGCAACACT+GGG 0.645980 4.4:-61178460 MS.gene34829:CDS
CAACACCGATGGAATTCATG+TGG 0.652375 4.4:-61177859 MS.gene34829:CDS
TTCCAATGTAGTAGTGAACA+GGG 0.653388 4.4:-61176902 MS.gene34829:CDS
CGAGCTATAGATATCACTTG+CGG 0.678372 4.4:-61177628 MS.gene34829:CDS
GTGATTCTAGAACCAGAAGG+TGG 0.703740 4.4:-61176105 MS.gene34829:CDS
GATGTGATTCTAGAACCAGA+AGG 0.739033 4.4:-61176108 MS.gene34829:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAACAATAACATATA+TGG - chr4.4:61176299-61176318 MS.gene34829:CDS 10.0%
!! AAAATATAACAAATGAAAAT+TGG + chr4.4:61177079-61177098 None:intergenic 10.0%
!! ACATTCAAAAAATAAATATT+TGG - chr4.4:61177623-61177642 MS.gene34829:CDS 10.0%
!! AATGGAAAATAAATTTATGT+AGG - chr4.4:61177356-61177375 MS.gene34829:intron 15.0%
!! AATTTCAACATATATGAAAA+AGG - chr4.4:61178000-61178019 MS.gene34829:CDS 15.0%
!! ATATAAAAAAATGTCTTAAC+CGG + chr4.4:61178173-61178192 None:intergenic 15.0%
!! ATTTAGAAGAAATTAATAAG+TGG + chr4.4:61178866-61178885 None:intergenic 15.0%
!! ATTTCAACATATATGAAAAA+GGG - chr4.4:61178001-61178020 MS.gene34829:CDS 15.0%
!! CTAAATAAAAAACATTCAAT+TGG - chr4.4:61177649-61177668 MS.gene34829:CDS 15.0%
!! TTTCTATGGTTATTAATTAA+AGG + chr4.4:61178902-61178921 None:intergenic 15.0%
!!! AAAAGAATGATTTCAAATTT+TGG - chr4.4:61177135-61177154 MS.gene34829:intron 15.0%
!!! ATACTTGTTGTAAAAATTTA+TGG + chr4.4:61177311-61177330 None:intergenic 15.0%
!!! ATTATATTTGACTTTTTAAC+CGG - chr4.4:61178135-61178154 MS.gene34829:intron 15.0%
!!! ATTGATTAAAACTTTTTCTT+TGG - chr4.4:61177862-61177881 MS.gene34829:CDS 15.0%
!!! GTTAAGACATTTTTTTATAT+GGG - chr4.4:61178173-61178192 MS.gene34829:intron 15.0%
!!! TCTAACATATATTTTTTTGA+GGG - chr4.4:61177973-61177992 MS.gene34829:CDS 15.0%
!!! TTCTAACATATATTTTTTTG+AGG - chr4.4:61177972-61177991 MS.gene34829:CDS 15.0%
!! AAATTGGAAAGAGAAAAAAA+AGG + chr4.4:61177063-61177082 None:intergenic 20.0%
!! AATGTTAGATATGTAAATAG+AGG - chr4.4:61179320-61179339 MS.gene34829:CDS 20.0%
!! ACATAATAAACTGTGATATT+GGG - chr4.4:61177559-61177578 MS.gene34829:intron 20.0%
!! CAATGTAATATTACTACATT+TGG - chr4.4:61178304-61178323 MS.gene34829:intron 20.0%
!! CAGAAAAGGAAATATAAAAA+GGG + chr4.4:61178375-61178394 None:intergenic 20.0%
!! TAATATATATTCTGCAGTAT+TGG - chr4.4:61178419-61178438 MS.gene34829:CDS 20.0%
!!! AAAACAAAACATCATCATTT+TGG - chr4.4:61176167-61176186 MS.gene34829:CDS 20.0%
!!! AAAACTTTAAAGTTGAGATT+GGG - chr4.4:61177899-61177918 MS.gene34829:CDS 20.0%
!!! ATTAAAACTTTTTCTTTGGA+GGG - chr4.4:61177866-61177885 MS.gene34829:CDS 20.0%
!!! ATTTTGAAAATTAGTCTGTT+AGG - chr4.4:61178563-61178582 MS.gene34829:intron 20.0%
!!! CTAATTGATTCATACTTTAA+TGG - chr4.4:61177338-61177357 MS.gene34829:intron 20.0%
!!! GGTTAAGACATTTTTTTATA+TGG - chr4.4:61178172-61178191 MS.gene34829:intron 20.0%
!!! TAAAACTTTAAAGTTGAGAT+TGG - chr4.4:61177898-61177917 MS.gene34829:CDS 20.0%
!!! TAATGAATTGATTTTGAACA+TGG + chr4.4:61176823-61176842 None:intergenic 20.0%
!!! TAGTATTGATCTATGATTAT+GGG + chr4.4:61179098-61179117 None:intergenic 20.0%
!!! TCAGTAATGCAATTTTAAAT+TGG + chr4.4:61176578-61176597 None:intergenic 20.0%
!!! TTCATTTAATTTCTCCTTTA+AGG - chr4.4:61176393-61176412 MS.gene34829:intron 20.0%
!!! TTTACTTTCTTTTTTGTTGA+AGG - chr4.4:61177686-61177705 MS.gene34829:intron 20.0%
! AAAACTTACAAGGTTATTGT+TGG + chr4.4:61177036-61177055 None:intergenic 25.0%
! ATAACCGATAAAACAAACAT+TGG - chr4.4:61178725-61178744 MS.gene34829:intron 25.0%
! ATATTGTAATAAAACTCACG+TGG + chr4.4:61178980-61178999 None:intergenic 25.0%
! ATTTGAGAGTAAAGAATCTA+AGG - chr4.4:61177403-61177422 MS.gene34829:intron 25.0%
! CATTGAAACTTATGCATTTA+AGG + chr4.4:61178289-61178308 None:intergenic 25.0%
! CCAGAAAAGGAAATATAAAA+AGG + chr4.4:61178376-61178395 None:intergenic 25.0%
! CGTAATTAACTGAATTATTG+AGG + chr4.4:61176687-61176706 None:intergenic 25.0%
! CTCAATCTAAGAATTAAATG+AGG + chr4.4:61177817-61177836 None:intergenic 25.0%
! GACATAATAAACTGTGATAT+TGG - chr4.4:61177558-61177577 MS.gene34829:intron 25.0%
! GGAAAATAAATTTATGTAGG+CGG - chr4.4:61177359-61177378 MS.gene34829:intron 25.0%
! TTACGTTACTATGGATAAAT+CGG - chr4.4:61176702-61176721 MS.gene34829:intron 25.0%
! TTACTTTGTGAACTAATCTA+TGG + chr4.4:61176751-61176770 None:intergenic 25.0%
! TTATGAAAAATACTTGAGAG+AGG - chr4.4:61177112-61177131 MS.gene34829:intron 25.0%
! TTCAGTTAATTACGTTACTA+TGG - chr4.4:61176693-61176712 MS.gene34829:intron 25.0%
!! AAAAGGTACAAAAACTTACA+AGG + chr4.4:61177046-61177065 None:intergenic 25.0%
!! CCTTTTTATATTTCCTTTTC+TGG - chr4.4:61178373-61178392 MS.gene34829:CDS 25.0%
!! TAAGCTATTTGCCAAATTTT+AGG - chr4.4:61178699-61178718 MS.gene34829:intron 25.0%
!! TTGATTTTGAACATGGTATA+AGG + chr4.4:61176816-61176835 None:intergenic 25.0%
!!! AGTATTTTGGTCAATTTGTA+TGG + chr4.4:61178957-61178976 None:intergenic 25.0%
!!! CTTTCCAATGTTTGTTTTAT+CGG + chr4.4:61178732-61178751 None:intergenic 25.0%
!!! GATTAAAACTTTTTCTTTGG+AGG - chr4.4:61177865-61177884 MS.gene34829:CDS 25.0%
!!! GTAGTATTGATCTATGATTA+TGG + chr4.4:61179099-61179118 None:intergenic 25.0%
!!! GTATTTTGGTCAATTTGTAT+GGG + chr4.4:61178956-61178975 None:intergenic 25.0%
!!! TGATGTTTTTGTACAAAACT+TGG + chr4.4:61179172-61179191 None:intergenic 25.0%
ACATATATGGCAACAAAAAC+AGG - chr4.4:61176312-61176331 MS.gene34829:CDS 30.0%
AGATGGTATATCCTATTCTT+GGG + chr4.4:61176472-61176491 None:intergenic 30.0%
ATGGAGTGAGAATAAAAACT+TGG - chr4.4:61178519-61178538 MS.gene34829:intron 30.0%
GAACATAGAAATGCAAAATG+TGG - chr4.4:61179067-61179086 MS.gene34829:intron 30.0%
GAAGAATATGATCTTACAGT+TGG + chr4.4:61177285-61177304 None:intergenic 30.0%
GATTGTATTTCGATAGTTAG+TGG - chr4.4:61177713-61177732 MS.gene34829:intron 30.0%
GTCTCAAATTTCAAAGTTGA+TGG - chr4.4:61176954-61176973 MS.gene34829:intron 30.0%
GTTATTGTTGGAATATTTGC+AGG + chr4.4:61177024-61177043 None:intergenic 30.0%
TATGATTCCATATGATCACA+GGG - chr4.4:61179011-61179030 MS.gene34829:intron 30.0%
TTATGATTCCATATGATCAC+AGG - chr4.4:61179010-61179029 MS.gene34829:intron 30.0%
TTCACAAAGTAAAGCTTAGT+GGG - chr4.4:61176759-61176778 MS.gene34829:intron 30.0%
! ATCATGAGAAGTGTGATAAT+TGG + chr4.4:61176044-61176063 None:intergenic 30.0%
! ATCTATAGCTCGAACATTTT+TGG + chr4.4:61177741-61177760 None:intergenic 30.0%
! ATTTTGAACATGGTATAAGG+AGG + chr4.4:61176813-61176832 None:intergenic 30.0%
! GTTTTGTACAAAAACATCAG+AGG - chr4.4:61179173-61179192 MS.gene34829:CDS 30.0%
!! AAAAAGTTATTGGCTTGAGA+TGG + chr4.4:61176489-61176508 None:intergenic 30.0%
!! AATTCATCATGTTAGCTCTA+TGG - chr4.4:61176861-61176880 MS.gene34829:CDS 30.0%
!! ATCATTTTGGTCACATAACT+AGG - chr4.4:61176180-61176199 MS.gene34829:CDS 30.0%
!! TCAAATTTCAAAGTTGATGG+TGG - chr4.4:61176957-61176976 MS.gene34829:intron 30.0%
AAAACTTGGCAGGTAAAAGT+TGG - chr4.4:61178533-61178552 MS.gene34829:intron 35.0%
AAGTGGATAAAACTTGTCAC+AGG + chr4.4:61178849-61178868 None:intergenic 35.0%
ACCCTCAATAACATTACTGA+CGG - chr4.4:61176654-61176673 MS.gene34829:intron 35.0%
AGTGAGAATAAAAACTTGGC+AGG - chr4.4:61178523-61178542 MS.gene34829:intron 35.0%
ATAAACTGTGATATTGGGAC+AGG - chr4.4:61177564-61177583 MS.gene34829:intron 35.0%
ATGGTAGTAACGTTCAACAT+TGG + chr4.4:61176126-61176145 None:intergenic 35.0%
CAAAACTTGGCTTAATTGCA+CGG + chr4.4:61179159-61179178 None:intergenic 35.0%
CAGATTTGTCAATTGAGTAC+TGG + chr4.4:61176728-61176747 None:intergenic 35.0%
CATGGACCTGAAAAAGTTAT+TGG + chr4.4:61176499-61176518 None:intergenic 35.0%
CTCTATAAGTAGTCCAGAAA+AGG + chr4.4:61178389-61178408 None:intergenic 35.0%
GAGATGGTATATCCTATTCT+TGG + chr4.4:61176473-61176492 None:intergenic 35.0%
GCTTCATTACTCATATGCAA+TGG - chr4.4:61176071-61176090 MS.gene34829:CDS 35.0%
GTTAGGTAGTTTGAACTAGA+CGG + chr4.4:61178822-61178841 None:intergenic 35.0%
GTTCACAAAGTAAAGCTTAG+TGG - chr4.4:61176758-61176777 MS.gene34829:intron 35.0%
TAGTGTAAGACTCACCATAA+AGG + chr4.4:61176536-61176555 None:intergenic 35.0%
TCAACCAAATACTGCCTTTA+TGG - chr4.4:61176519-61176538 MS.gene34829:intron 35.0%
TCAATCTACCATCATGTACA+TGG + chr4.4:61176145-61176164 None:intergenic 35.0%
TCAATGGCAATGGGAAAAAA+TGG - chr4.4:61176988-61177007 MS.gene34829:intron 35.0%
TCCATTAGTTGTTCCTGTTA+AGG + chr4.4:61178504-61178523 None:intergenic 35.0%
TCCGTCAGTAATGTTATTGA+GGG + chr4.4:61176658-61176677 None:intergenic 35.0%
TCGGTTATGCACCTAAAATT+TGG + chr4.4:61178713-61178732 None:intergenic 35.0%
TGAAAAAAGGACCAACAAAG+TGG + chr4.4:61178790-61178809 None:intergenic 35.0%
TGAAAAAGGGTAATGCTAAC+CGG - chr4.4:61178014-61178033 MS.gene34829:intron 35.0%
TGCAAGTATATTTCTCTGCA+AGG + chr4.4:61179248-61179267 None:intergenic 35.0%
TGCATATGAGTAATGAAGCT+AGG + chr4.4:61176070-61176089 None:intergenic 35.0%
TGCCTTCTTAAATGCCTTAA+AGG + chr4.4:61176410-61176429 None:intergenic 35.0%
TGGAATGAAGCATGTTCTAA+AGG - chr4.4:61176430-61176449 MS.gene34829:intron 35.0%
TGTCAGCATATGAAGAAGAT+AGG - chr4.4:61176907-61176926 MS.gene34829:CDS 35.0%
TTCCAATGTAGTAGTGAACA+GGG - chr4.4:61178475-61178494 MS.gene34829:CDS 35.0%
TTCCGTCAGTAATGTTATTG+AGG + chr4.4:61176659-61176678 None:intergenic 35.0%
TTGACAAATCTCCACTTTGT+TGG - chr4.4:61178776-61178795 MS.gene34829:intron 35.0%
TTGCAGAGAAATATACTTGC+AGG - chr4.4:61179247-61179266 MS.gene34829:CDS 35.0%
TTTCCAATGTAGTAGTGAAC+AGG - chr4.4:61178474-61178493 MS.gene34829:CDS 35.0%
! AATGCTACATGCATTTGTTG+TGG + chr4.4:61177438-61177457 None:intergenic 35.0%
! AGGAGAAGAGCAATTTTCTA+TGG + chr4.4:61178916-61178935 None:intergenic 35.0%
! CTTTTCTCTTCAAAGTCTCA+AGG - chr4.4:61176218-61176237 MS.gene34829:CDS 35.0%
! GGAATGAAGCATGTTCTAAA+GGG - chr4.4:61176431-61176450 MS.gene34829:intron 35.0%
! GGTTTTTGTTGCATTACTGA+AGG + chr4.4:61176637-61176656 None:intergenic 35.0%
! TATGATCTTACAGTTGGTCT+TGG + chr4.4:61177279-61177298 None:intergenic 35.0%
! TTAAGGCATTTAAGAAGGCA+TGG - chr4.4:61176410-61176429 MS.gene34829:intron 35.0%
! TTCTGCAGTATTGGAAGTTT+AGG - chr4.4:61178428-61178447 MS.gene34829:CDS 35.0%
!! AAAACTCACGTGGAGTATTT+TGG + chr4.4:61178970-61178989 None:intergenic 35.0%
!! AATTTCAAAGTTGATGGTGG+TGG - chr4.4:61176960-61176979 MS.gene34829:intron 35.0%
!! ACGGTTTGGTCAATGAAAAA+AGG + chr4.4:61178803-61178822 None:intergenic 35.0%
!! ATGGTTAGTGTTGATGACTT+TGG - chr4.4:61176242-61176261 MS.gene34829:CDS 35.0%
!! CTCAAGCCAATAACTTTTTC+AGG - chr4.4:61176490-61176509 MS.gene34829:intron 35.0%
!! CTCCTTTAAGGCATTTAAGA+AGG - chr4.4:61176405-61176424 MS.gene34829:intron 35.0%
!! TTAGCTCTATGGAACAATTG+AGG - chr4.4:61176872-61176891 MS.gene34829:CDS 35.0%
AACTTGTCACAGGAGTTGTT+AGG + chr4.4:61178839-61178858 None:intergenic 40.0%
AAGGCAGTATTTGGTTGACA+TGG + chr4.4:61176517-61176536 None:intergenic 40.0%
AGACCCTCTGTAATTACTGA+CGG - chr4.4:61176605-61176624 MS.gene34829:intron 40.0%
AGACCGTCAGTAATTACAGA+GGG + chr4.4:61176611-61176630 None:intergenic 40.0%
AGATGGAAGAGATGATAGAG+AGG - chr4.4:61176274-61176293 MS.gene34829:CDS 40.0%
ATGAAACCTGATTCCATGTC+CGG + chr4.4:61177777-61177796 None:intergenic 40.0%
ATGAAGAAGATAGGCAACAC+TGG - chr4.4:61176916-61176935 MS.gene34829:CDS 40.0%
ATGCAATGGCTCTTACATAG+TGG - chr4.4:61176085-61176104 MS.gene34829:CDS 40.0%
ATGGCAATGGGAAAAAATGG+TGG - chr4.4:61176991-61177010 MS.gene34829:intron 40.0%
CGAGCTATAGATATCACTTG+CGG - chr4.4:61177749-61177768 MS.gene34829:intron 40.0%
CGTTACTACCATGTACATGA+TGG - chr4.4:61176134-61176153 MS.gene34829:CDS 40.0%
CTCACCATAAAGGCAGTATT+TGG + chr4.4:61176526-61176545 None:intergenic 40.0%
CTCCTCTCTAATTTCGTATG+AGG + chr4.4:61177161-61177180 None:intergenic 40.0%
CTCTTCAAAGTCTCAAGGAA+TGG - chr4.4:61176223-61176242 MS.gene34829:CDS 40.0%
GCCTTAACAGGAACAACTAA+TGG - chr4.4:61178500-61178519 MS.gene34829:CDS 40.0%
GTAGTTTGAACTAGACGGTT+TGG + chr4.4:61178817-61178836 None:intergenic 40.0%
TAGACCGTCAGTAATTACAG+AGG + chr4.4:61176612-61176631 None:intergenic 40.0%
TCTATGGAACAATTGAGGCA+TGG - chr4.4:61176877-61176896 MS.gene34829:CDS 40.0%
TGAAGAAGATAGGCAACACT+GGG - chr4.4:61176917-61176936 MS.gene34829:CDS 40.0%
TTCAGCCACATGAATTCCAT+CGG + chr4.4:61177526-61177545 None:intergenic 40.0%
! ACATGAATTCCATCGGTGTT+GGG + chr4.4:61177519-61177538 None:intergenic 40.0%
! TTCTAAAGGGTCTATCCTTG+TGG - chr4.4:61176444-61176463 MS.gene34829:intron 40.0%
!! GATGTGATTCTAGAACCAGA+AGG - chr4.4:61179269-61179288 MS.gene34829:CDS 40.0%
!! TCAACATTGGCTTGATGAAG+TGG + chr4.4:61176113-61176132 None:intergenic 40.0%
!! TGTCTCTAGGGCATTAGTTA+AGG - chr4.4:61178037-61178056 MS.gene34829:intron 40.0%
AACTAATGCCCTAGAGACAC+CGG + chr4.4:61178036-61178055 None:intergenic 45.0%
AATGCTAACCGGTGTCTCTA+GGG - chr4.4:61178025-61178044 MS.gene34829:intron 45.0%
AGCCCTGTTCACTACTACAT+TGG + chr4.4:61178480-61178499 None:intergenic 45.0%
ATAGATATCACTTGCGGACC+CGG - chr4.4:61177755-61177774 MS.gene34829:intron 45.0%
ATATGATCACAGGGTGGTTC+AGG - chr4.4:61179020-61179039 MS.gene34829:intron 45.0%
CAACACCGATGGAATTCATG+TGG - chr4.4:61177518-61177537 MS.gene34829:intron 45.0%
CACATGAATTCCATCGGTGT+TGG + chr4.4:61177520-61177539 None:intergenic 45.0%
GATTCCATATGATCACAGGG+TGG - chr4.4:61179014-61179033 MS.gene34829:intron 45.0%
GGAAATGTTAACTAGTGCCC+TGG + chr4.4:61178354-61178373 None:intergenic 45.0%
GGCCTCATACGAAATTAGAG+AGG - chr4.4:61177156-61177175 MS.gene34829:intron 45.0%
GGCTGTGTTCAACAAGAAGA+GGG + chr4.4:61177243-61177262 None:intergenic 45.0%
GGTCTTGGTTCATCATTGCA+TGG + chr4.4:61177264-61177283 None:intergenic 45.0%
GTGGCACTTTCAATGGCAAT+GGG - chr4.4:61176979-61176998 MS.gene34829:intron 45.0%
TAATGCTAACCGGTGTCTCT+AGG - chr4.4:61178024-61178043 MS.gene34829:intron 45.0%
TGAAACCTGATTCCATGTCC+GGG + chr4.4:61177776-61177795 None:intergenic 45.0%
TGAACCACCCTGTGATCATA+TGG + chr4.4:61179021-61179040 None:intergenic 45.0%
TGGCTGTGTTCAACAAGAAG+AGG + chr4.4:61177244-61177263 None:intergenic 45.0%
TTTCTCTGCAAGGAACGCTT+CGG + chr4.4:61179238-61179257 None:intergenic 45.0%
!! ATTCTAGAACCAGAAGGTGG+TGG - chr4.4:61179275-61179294 MS.gene34829:CDS 45.0%
!! GTGATTCTAGAACCAGAAGG+TGG - chr4.4:61179272-61179291 MS.gene34829:CDS 45.0%
!!! GGGAGTGAGCGTTTTTTGTT+AGG + chr4.4:61178936-61178955 None:intergenic 45.0%
AAACAGAGCCATGCCAAGAG+CGG - chr4.4:61179126-61179145 MS.gene34829:CDS 50.0%
AATGTCTTAACCGGTGCCTC+TGG + chr4.4:61178164-61178183 None:intergenic 50.0%
ATGTCTTAACCGGTGCCTCT+GGG + chr4.4:61178163-61178182 None:intergenic 50.0%
GGTGGCACTTTCAATGGCAA+TGG - chr4.4:61176978-61176997 MS.gene34829:intron 50.0%
TCCTATTCTTGGGCACCACA+AGG + chr4.4:61176462-61176481 None:intergenic 50.0%
TCCTTGTGGTGCCCAAGAAT+AGG - chr4.4:61176458-61176477 MS.gene34829:intron 50.0%
! ACTTTTTAACCGGTGCCCAG+AGG - chr4.4:61178145-61178164 MS.gene34829:intron 50.0%
!! GACTTTGGAGCAAGAGCAGA+TGG - chr4.4:61176257-61176276 MS.gene34829:CDS 50.0%
!! GATGGTGGTGGCACTTTCAA+TGG - chr4.4:61176972-61176991 MS.gene34829:intron 50.0%
!! GGTCATTTTAACCAGTGCCC+CGG - chr4.4:61178194-61178213 MS.gene34829:intron 50.0%
!! GTCATTTTAACCAGTGCCCC+GGG - chr4.4:61178195-61178214 MS.gene34829:intron 50.0%
!! TCATTTTAACCAGTGCCCCG+GGG - chr4.4:61178196-61178215 MS.gene34829:intron 50.0%
!! TTTGATTGTGAGAGCGCCAG+AGG - chr4.4:61177488-61177507 MS.gene34829:intron 50.0%
AACTAGTGCCCTGGATCCCA+GGG + chr4.4:61178345-61178364 None:intergenic 55.0%
ACTTCCTTAACCAGTGCCCC+CGG + chr4.4:61178216-61178235 None:intergenic 55.0%
GAGGATAGTCCCAACACCGA+TGG - chr4.4:61177507-61177526 MS.gene34829:intron 55.0%
GCTTCCTTAACCAGTGCCCT+GGG - chr4.4:61178326-61178345 MS.gene34829:intron 55.0%
GTAGTTCCTCCACCACCTTC+TGG + chr4.4:61179287-61179306 None:intergenic 55.0%
GTGAACAGGGCTGCCTTAAC+AGG - chr4.4:61178488-61178507 MS.gene34829:CDS 55.0%
TAACTAGTGCCCTGGATCCC+AGG + chr4.4:61178346-61178365 None:intergenic 55.0%
! CTAGAACCAGAAGGTGGTGG+AGG - chr4.4:61179278-61179297 MS.gene34829:CDS 55.0%
! GCTCTTGGCATGGCTCTGTT+TGG + chr4.4:61179127-61179146 None:intergenic 55.0%
! GCTTTGTTCCGCTCTTGGCA+TGG + chr4.4:61179137-61179156 None:intergenic 55.0%
!! CATTTTAACCAGTGCCCCGG+GGG - chr4.4:61178197-61178216 MS.gene34829:intron 55.0%
!! TCGGTGTTGGGACTATCCTC+TGG + chr4.4:61177507-61177526 None:intergenic 55.0%
AACCAGTGCCCTGGGATCCA+GGG - chr4.4:61178334-61178353 MS.gene34829:intron 60.0%
ATCACTTGCGGACCCGGACA+TGG - chr4.4:61177761-61177780 MS.gene34829:intron 60.0%
CTTCCTTAACCAGTGCCCCC+GGG + chr4.4:61178215-61178234 None:intergenic 60.0%
GGCTTCCTTAACCAGTGCCC+TGG - chr4.4:61178325-61178344 MS.gene34829:intron 60.0%
TAACCAGTGCCCTGGGATCC+AGG - chr4.4:61178333-61178352 MS.gene34829:intron 60.0%
TTCCTTAACCAGTGCCCCCG+GGG + chr4.4:61178214-61178233 None:intergenic 60.0%
!! GCACGGCTTTGTTCCGCTCT+TGG + chr4.4:61179142-61179161 None:intergenic 60.0%
!! GGATCCCAGGGCACTGGTTA+AGG + chr4.4:61178333-61178352 None:intergenic 60.0%
GCGGACCCGGACATGGAATC+AGG - chr4.4:61177768-61177787 MS.gene34829:intron 65.0%
TAACCGGTGCCTCTGGGCAC+CGG + chr4.4:61178157-61178176 None:intergenic 65.0%
! TAACCGGTGCCCAGAGGCAC+CGG - chr4.4:61178151-61178170 MS.gene34829:intron 65.0%
TGCCCTGGATCCCAGGGCAC+TGG + chr4.4:61178339-61178358 None:intergenic 70.0%
!! TGCCCCGGGGGCACTGGTTA+AGG - chr4.4:61178209-61178228 MS.gene34829:intron 70.0%
AACCAGTGCCCCCGGGGCAC+TGG + chr4.4:61178208-61178227 None:intergenic 75.0%
! AACCAGTGCCCCGGGGGCAC+TGG - chr4.4:61178203-61178222 MS.gene34829:intron 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 61176026 61179373 61176026 ID=MS.gene34829
chr4.4 mRNA 61176026 61179373 61176026 ID=MS.gene34829.t1;Parent=MS.gene34829
chr4.4 exon 61179104 61179373 61179104 ID=MS.gene34829.t1.exon1;Parent=MS.gene34829.t1
chr4.4 CDS 61179104 61179373 61179104 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
chr4.4 exon 61178859 61178984 61178859 ID=MS.gene34829.t1.exon2;Parent=MS.gene34829.t1
chr4.4 CDS 61178859 61178984 61178859 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
chr4.4 exon 61178353 61178523 61178353 ID=MS.gene34829.t1.exon3;Parent=MS.gene34829.t1
chr4.4 CDS 61178353 61178523 61178353 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
chr4.4 exon 61178112 61178138 61178112 ID=MS.gene34829.t1.exon4;Parent=MS.gene34829.t1
chr4.4 CDS 61178112 61178138 61178112 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
chr4.4 exon 61177814 61178021 61177814 ID=MS.gene34829.t1.exon5;Parent=MS.gene34829.t1
chr4.4 CDS 61177814 61178021 61177814 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
chr4.4 exon 61177610 61177691 61177610 ID=MS.gene34829.t1.exon6;Parent=MS.gene34829.t1
chr4.4 CDS 61177610 61177691 61177610 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
chr4.4 exon 61176855 61176963 61176855 ID=MS.gene34829.t1.exon7;Parent=MS.gene34829.t1
chr4.4 CDS 61176855 61176963 61176855 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
chr4.4 exon 61176026 61176367 61176026 ID=MS.gene34829.t1.exon8;Parent=MS.gene34829.t1
chr4.4 CDS 61176026 61176367 61176026 ID=cds.MS.gene34829.t1;Parent=MS.gene34829.t1
Gene Sequence

>MS.gene34829

ATGTATCAACAAAAGCCAATTATCACACTTCTCATGATATTCCTAGCTTCATTACTCATATGCAATGGCTCTTACATAGTGGAACCACTTCATCAAGCCAATGTTGAACGTTACTACCATGTACATGATGGTAGATTGATCAAAACAAAACATCATCATTTTGGTCACATAACTAGGACAAATAGAGATACACTTTTCTCTTCAAAGTCTCAAGGAATGGTTAGTGTTGATGACTTTGGAGCAAGAGCAGATGGAAGAGATGATAGAGAGGTAAAAAATAACAATAACATATATGGCAACAAAAACAGGAAAATTTATTATGTTAATTCACTAACAACAATATTCTCAAACTTTCTCACTTTTTCATTTCATTTAATTTCTCCTTTAAGGCATTTAAGAAGGCATGGAATGAAGCATGTTCTAAAGGGTCTATCCTTGTGGTGCCCAAGAATAGGATATACCATCTCAAGCCAATAACTTTTTCAGGTCCATGTCAACCAAATACTGCCTTTATGGTGAGTCTTACACTACAAGAATTCTCTTTATTGACCAATTTAAAATTGCATTACTGAATTATTTAGACCCTCTGTAATTACTGACGGTCTAAACCTTCAGTAATGCAACAAAAACCCTCAATAACATTACTGACGGAATAAATCCTCAATAATTCAGTTAATTACGTTACTATGGATAAATCGGCCAGTACTCAATTGACAAATCTGCCATAGATTAGTTCACAAAGTAAAGCTTAGTGGGACACTCAATTAGATAGTTTTTTTTTTTTCCTCCTTATACCATGTTCAAAATCAATTCATTATAATTGTTTTATTTTTCGAATTCATCATGTTAGCTCTATGGAACAATTGAGGCATGGTCTCAAATGTCAGCATATGAAGAAGATAGGCAACACTGGGTTGTGTTTGATAGTGTCTCAAATTTCAAAGTTGATGGTGGTGGCACTTTCAATGGCAATGGGAAAAAATGGTGGCAAAACTCCTGCAAATATTCCAACAATAACCTTGTAAGTTTTTGTACCTTTTTTTTCTCTTTCCAATTTTCATTTGTTATATTTTCTTTTAAACTAAATTATGAAAAATACTTGAGAGAGGAAAAGAATGATTTCAAATTTTGGCCTCATACGAAATTAGAGAGGAGTTATATTTTATTTGTCATCAACTTTTTCTATTAGATGAATTTTGAACTTAATTTATTTTCCCTCTTCTTGTTGAACACAGCCATGCAATGATGAACCAAGACCAACTGTAAGATCATATTCTTCAAACCATAAATTTTTACAACAAGTATTTCTTTTCTAATTGATTCATACTTTAATGGAAAATAAATTTATGTAGGCGGTGACATTCTATGATTGCAAAAATTTGAGAGTAAAGAATCTAAGGTTCAAAGATCCACAACAAATGCATGTAGCATTTGAGAGATGCTTCAATGTGTTTGTTTCGAATTTGATTGTGAGAGCGCCAGAGGATAGTCCCAACACCGATGGAATTCATGTGGCTGAAACACAAAATATTGACATAATAAACTGTGATATTGGGACAGGTATGTATAAAAAAAGAGAAAATATTTCAAATAAAAAACATTCAAAAAATAAATATTTGGTTGCTAAATAAAAAACATTCAATTGGATTTTAATTTTTATTTTACTTTCTTTTTTGTTGAAGGTGATGATTGTATTTCGATAGTTAGTGGATCCAAAAATGTTCGAGCTATAGATATCACTTGCGGACCCGGACATGGAATCAGGTTTCATTGTTATATTATATTAATCCTCATTTAATTCTTAGATTGAGTTTTGTTTTGATCAATTTTTAAGAGATTGATTAAAACTTTTTCTTTGGAGGGAAGATTGATTAAAACTTTAAAGTTGAGATTGGGAGAAGATTCTAGTCACTATAGATCTCAAAAATTAAGATGCAATAGATAATTTCTAACATATATTTTTTTGAGGGAAATAATTTCAACATATATGAAAAAGGGTAATGCTAACCGGTGTCTCTAGGGCATTAGTTAAGGAAGTCAAAAGAGAAAATTTAAAATAAAAAATGATATATTTGAAGTATTAATTACATAACTTTAAATGCAAATATTATTATATTTGACTTTTTAACCGGTGCCCAGAGGCACCGGTTAAGACATTTTTTTATATGGGTCATTTTAACCAGTGCCCCGGGGGCACTGGTTAAGGAAGTTAAAAAGAGAAAATTTTAAATTAAAAATGTCACTTTTTATACTTTTAATACCTTAAATGCATAAGTTTCAATGTAATATTACTACATTTGGCTTCCTTAACCAGTGCCCTGGGATCCAGGGCACTAGTTAACATTTCCCTTTTTATATTTCCTTTTCTGGACTACTTATAGAGAAGTACTCATTAATATATATTCTGCAGTATTGGAAGTTTAGGAGCTGATAACTCAGAAGCTGAAGTTTCCAATGTAGTAGTGAACAGGGCTGCCTTAACAGGAACAACTAATGGAGTGAGAATAAAAACTTGGCAGGTAAAAGTTGGTCTTAAGATTTTGAAAATTAGTCTGTTAGGCACTCTACAACTAACTTTGTGAGATGAAAGAATGAACTAATTAATAGAAGTCAAAAAATAAACTTTCATAAAGTAGAATTTCATCTAATCATGTAAATCTTGTTGACTTTCTATAAGCTATTTGCCAAATTTTAGGTGCATAACCGATAAAACAAACATTGGAAAGTTGATGAAATTAGTGCAAACAACTTTGACAAATCTCCACTTTGTTGGTCCTTTTTTCATTGACCAAACCGTCTAGTTCAAACTACCTAACAACTCCTGTGACAAGTTTTATCCACTTATTAATTTCTTCTAAATGTCTCAATGACGACCTTTAATTAATAACCATAGAAAATTGCTCTTCTCCTAACAAAAAACGCTCACTCCCATACAAATTGACCAAAATACTCCACGTGAGTTTTATTACAATATATATCTTATATTATGATTCCATATGATCACAGGGTGGTTCAGGATATGCAAGAAACATCAAATTTATGAACATAGAAATGCAAAATGTGGCAAATCCCATAATCATAGATCAATACTACTGCGACCAAACAGAGCCATGCCAAGAGCGGAACAAAGCCGTGCAATTAAGCCAAGTTTTGTACAAAAACATCAGAGGAACGAGTGCTTCAGAAGTAGCGATCAAATTTAATTGCAGCCGAAGCGTTCCTTGCAGAGAAATATACTTGCAGGATGTGATTCTAGAACCAGAAGGTGGTGGAGGAACTACTGCAACATGTGAGAATGTTAGATATGTAAATAGAGGAAAGTTTTATCCTCAATGCTCTCCACAATAG
Protein sequence

>MS.gene34829.t1

MYQQKPIITLLMIFLASLLICNGSYIVEPLHQANVERYYHVHDGRLIKTKHHHFGHITRTNRDTLFSSKSQGMVSVDDFGARADGRDDREAFKKAWNEACSKGSILVVPKNRIYHLKPITFSGPCQPNTAFMLYGTIEAWSQMSAYEEDRQHWVVFDSVSNFKVDGGGTFNGNGKKWWQNSCKYSNNNLPCNDEPRPTAVTFYDCKNLRVKNLRFKDPQQMHVAFERCFNVFVSNLIVRAPEDSPNTDGIHVAETQNIDIINCDIGTGDDCISIVSGSKNVRAIDITCGPGHGISIGSLGADNSEAEVSNVVVNRAALTGTTNGVRIKTWQGGSGYARNIKFMNIEMQNVANPIIIDQYYCDQTEPCQERNKAVQLSQVLYKNIRGTSASEVAIKFNCSRSVPCREIYLQDVILEPEGGGGTTATCENVRYVNRGKFYPQCSPQ