Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35699.t1 | XP_003596834.1 | 100 | 80 | 0 | 0 | 28 | 107 | 28 | 107 | 2.90E-34 | 154.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35699.t1 | Q9SIK2 | 92.5 | 80 | 6 | 0 | 28 | 107 | 29 | 108 | 4.9e-35 | 148.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35699.t1 | B7FGM7 | 100.0 | 80 | 0 | 0 | 28 | 107 | 28 | 107 | 2.1e-34 | 154.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene35699 | 0.829555 | 4.82E-55 | -1.69E-46 |
| MS.gene051850 | MS.gene35699 | 0.814891 | 1.22E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene35699 | 0.801112 | 1.05E-48 | -1.69E-46 |
| MS.gene054937 | MS.gene35699 | 0.852416 | 4.68E-61 | -1.69E-46 |
| MS.gene056075 | MS.gene35699 | 0.829144 | 6.07E-55 | -1.69E-46 |
| MS.gene057094 | MS.gene35699 | 0.812956 | 3.26E-51 | -1.69E-46 |
| MS.gene059726 | MS.gene35699 | 0.80124 | 9.89E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene35699 | 0.819734 | 9.94E-53 | -1.69E-46 |
| MS.gene061145 | MS.gene35699 | 0.827648 | 1.39E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35699.t1 | MTR_2g086610 | 100.000 | 107 | 0 | 0 | 1 | 107 | 1 | 107 | 8.17e-75 | 216 |
| MS.gene35699.t1 | MTR_4g070600 | 99.065 | 107 | 1 | 0 | 1 | 107 | 1 | 107 | 2.56e-74 | 215 |
| MS.gene35699.t1 | MTR_4g070600 | 98.131 | 107 | 1 | 1 | 1 | 107 | 1 | 106 | 7.23e-72 | 209 |
| MS.gene35699.t1 | MTR_6g015615 | 80.556 | 72 | 4 | 2 | 2 | 72 | 13 | 75 | 2.60e-17 | 70.9 |
| MS.gene35699.t1 | MTR_3g117040 | 80.556 | 72 | 4 | 2 | 2 | 72 | 13 | 75 | 2.60e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35699.t1 | AT2G21580 | 91.667 | 108 | 8 | 1 | 1 | 107 | 1 | 108 | 2.32e-53 | 162 |
| MS.gene35699.t1 | AT4G39200 | 88.889 | 108 | 11 | 1 | 1 | 107 | 1 | 108 | 8.84e-52 | 158 |
| MS.gene35699.t1 | AT2G21580 | 87.963 | 108 | 11 | 2 | 1 | 107 | 1 | 107 | 1.04e-51 | 158 |
| MS.gene35699.t1 | AT4G39200 | 87.037 | 108 | 12 | 2 | 1 | 107 | 1 | 107 | 4.07e-50 | 154 |
| MS.gene35699.t1 | AT4G34555 | 88.785 | 107 | 11 | 1 | 1 | 106 | 1 | 107 | 9.35e-50 | 153 |
| MS.gene35699.t1 | AT2G16360 | 85.714 | 105 | 14 | 1 | 2 | 105 | 2 | 106 | 2.92e-46 | 144 |
Find 29 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAGTGATGAGTTTGTATT+TGG | 0.207961 | 2.4:+13690679 | None:intergenic |
| GAAAGCAAAAGAAGAAGAAA+TGG | 0.344636 | 2.4:-13690784 | MS.gene35699:CDS |
| CAGTCCTCTCTGATCGTCTC+AGG | 0.361688 | 2.4:-13690655 | MS.gene35699:CDS |
| TGATGGCAAGAGGATTGATT+AGG | 0.387796 | 2.4:-13690511 | MS.gene35699:CDS |
| TCATCAAAGCCGGCGAAATC+AGG | 0.410202 | 2.4:-13690812 | MS.gene35699:CDS |
| GGTGGTGGTGCTGCCTTCTT+CGG | 0.414824 | 2.4:+13690836 | None:intergenic |
| TCGTGCAATCAGAGATTTGA+TGG | 0.416147 | 2.4:-13690528 | MS.gene35699:CDS |
| TGAAACCGCCTATGTATTAG+TGG | 0.440250 | 2.4:+13690448 | None:intergenic |
| AACATGGTCTTGTTTGATCA+AGG | 0.477202 | 2.4:-13690731 | MS.gene35699:CDS |
| CAAAGGTAAACAAAAGGAAA+AGG | 0.503611 | 2.4:-13690759 | MS.gene35699:CDS |
| TTCAGTGAGAAGCTTATCAT+AGG | 0.513766 | 2.4:+13690706 | None:intergenic |
| GATTTCGCCGGCTTTGATGA+CGG | 0.516878 | 2.4:+13690815 | None:intergenic |
| ATGGAGCAAAGGTAAACAAA+AGG | 0.525529 | 2.4:-13690765 | MS.gene35699:CDS |
| ACCACCACCGTCATCAAAGC+CGG | 0.527384 | 2.4:-13690822 | MS.gene35699:CDS |
| CACTAGAGCCACTAATACAT+AGG | 0.531061 | 2.4:-13690456 | None:intergenic |
| TTTGTTGTAGGCACCGAAGA+AGG | 0.534954 | 2.4:-13690849 | MS.gene35699:intron |
| GGAGTGATGAGTTTGTATTT+GGG | 0.540872 | 2.4:+13690680 | None:intergenic |
| ATCAGAGATTTGATGGCAAG+AGG | 0.560553 | 2.4:-13690521 | MS.gene35699:CDS |
| TTTCCTCCTCCTGATTTCGC+CGG | 0.569055 | 2.4:+13690803 | None:intergenic |
| GCCGGCTTTGATGACGGTGG+TGG | 0.569126 | 2.4:+13690821 | None:intergenic |
| AGACGATCAGAGAGGACTGA+AGG | 0.570313 | 2.4:+13690659 | None:intergenic |
| GGCAAGAGGATTGATTAGGT+TGG | 0.587429 | 2.4:-13690507 | MS.gene35699:CDS |
| AGTCCTCTCTGATCGTCTCA+GGG | 0.592148 | 2.4:-13690654 | MS.gene35699:intron |
| AAGAAGAAGAAATGGAGCAA+AGG | 0.611863 | 2.4:-13690776 | MS.gene35699:CDS |
| AAGCCGGCGAAATCAGGAGG+AGG | 0.614520 | 2.4:-13690806 | MS.gene35699:CDS |
| TCAAAGCCGGCGAAATCAGG+AGG | 0.640955 | 2.4:-13690809 | MS.gene35699:CDS |
| AAAGGAAAAGGTTAACAACA+TGG | 0.683005 | 2.4:-13690747 | MS.gene35699:CDS |
| TTCGCCGGCTTTGATGACGG+TGG | 0.725481 | 2.4:+13690818 | None:intergenic |
| ATACCCTGAGACGATCAGAG+AGG | 0.757534 | 2.4:+13690651 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTAATGTTTTCAGATTAA+TGG | - | chr2.4:13690833-13690852 | MS.gene35699:CDS | 20.0% |
| !!! | TTTTCAGATTAATGGATCTT+TGG | - | chr2.4:13690841-13690860 | MS.gene35699:CDS | 25.0% |
| AAAGGAAAAGGTTAACAACA+TGG | - | chr2.4:13690649-13690668 | MS.gene35699:intron | 30.0% | |
| CAAAGGTAAACAAAAGGAAA+AGG | - | chr2.4:13690637-13690656 | MS.gene35699:intron | 30.0% | |
| GAAAGCAAAAGAAGAAGAAA+TGG | - | chr2.4:13690612-13690631 | MS.gene35699:intron | 30.0% | |
| TAAGGAAAGGAATTTGATGA+TGG | + | chr2.4:13690483-13690502 | None:intergenic | 30.0% | |
| !! | ACTTCCATTTTCTTTGTTGT+AGG | - | chr2.4:13690535-13690554 | MS.gene35699:CDS | 30.0% |
| !! | AGGAATTTGATGATGGAAAT+TGG | + | chr2.4:13690476-13690495 | None:intergenic | 30.0% |
| !! | GAATTTGATGATGGAAATTG+GGG | + | chr2.4:13690474-13690493 | None:intergenic | 30.0% |
| !! | GGAATTTGATGATGGAAATT+GGG | + | chr2.4:13690475-13690494 | None:intergenic | 30.0% |
| AAGAAGAAGAAATGGAGCAA+AGG | - | chr2.4:13690620-13690639 | MS.gene35699:intron | 35.0% | |
| AGGAGTGATGAGTTTGTATT+TGG | + | chr2.4:13690720-13690739 | None:intergenic | 35.0% | |
| ATGGAGCAAAGGTAAACAAA+AGG | - | chr2.4:13690631-13690650 | MS.gene35699:intron | 35.0% | |
| CGAGATTTAGATCTAAGGAA+AGG | + | chr2.4:13690496-13690515 | None:intergenic | 35.0% | |
| GGAGTGATGAGTTTGTATTT+GGG | + | chr2.4:13690719-13690738 | None:intergenic | 35.0% | |
| TGCAACGAGATTTAGATCTA+AGG | + | chr2.4:13690501-13690520 | None:intergenic | 35.0% | |
| TTCAGTGAGAAGCTTATCAT+AGG | + | chr2.4:13690693-13690712 | None:intergenic | 35.0% | |
| !! | AACATGGTCTTGTTTGATCA+AGG | - | chr2.4:13690665-13690684 | MS.gene35699:CDS | 35.0% |
| GAATTGAATCGAAGCGACAA+AGG | + | chr2.4:13690796-13690815 | None:intergenic | 40.0% | |
| GGTGCCTACAACAAAGAAAA+TGG | + | chr2.4:13690542-13690561 | None:intergenic | 40.0% | |
| TCGTGCAATCAGAGATTTGA+TGG | - | chr2.4:13690868-13690887 | MS.gene35699:intron | 40.0% | |
| TGATGGCAAGAGGATTGATT+AGG | - | chr2.4:13690885-13690904 | MS.gene35699:intron | 40.0% | |
| ! | ATCAGAGATTTGATGGCAAG+AGG | - | chr2.4:13690875-13690894 | MS.gene35699:intron | 40.0% |
| !! | TTGATGATGGAAATTGGGGT+TGG | + | chr2.4:13690470-13690489 | None:intergenic | 40.0% |
| GGCAAGAGGATTGATTAGGT+TGG | - | chr2.4:13690889-13690908 | MS.gene35699:intron | 45.0% | |
| ! | ATGATGGAAATTGGGGTTGG+TGG | + | chr2.4:13690467-13690486 | None:intergenic | 45.0% |
| !! | TTTGTTGTAGGCACCGAAGA+AGG | - | chr2.4:13690547-13690566 | MS.gene35699:CDS | 45.0% |
| AGACGATCAGAGAGGACTGA+AGG | + | chr2.4:13690740-13690759 | None:intergenic | 50.0% | |
| AGTCCTCTCTGATCGTCTCA+GGG | - | chr2.4:13690742-13690761 | MS.gene35699:CDS | 50.0% | |
| ATACCCTGAGACGATCAGAG+AGG | + | chr2.4:13690748-13690767 | None:intergenic | 50.0% | |
| TCATCAAAGCCGGCGAAATC+AGG | - | chr2.4:13690584-13690603 | MS.gene35699:intron | 50.0% | |
| TTTCCTCCTCCTGATTTCGC+CGG | + | chr2.4:13690596-13690615 | None:intergenic | 50.0% | |
| !! | GATTTCGCCGGCTTTGATGA+CGG | + | chr2.4:13690584-13690603 | None:intergenic | 50.0% |
| ACCACCACCGTCATCAAAGC+CGG | - | chr2.4:13690574-13690593 | MS.gene35699:intron | 55.0% | |
| CAGTCCTCTCTGATCGTCTC+AGG | - | chr2.4:13690741-13690760 | MS.gene35699:CDS | 55.0% | |
| TCAAAGCCGGCGAAATCAGG+AGG | - | chr2.4:13690587-13690606 | MS.gene35699:intron | 55.0% | |
| AAGCCGGCGAAATCAGGAGG+AGG | - | chr2.4:13690590-13690609 | MS.gene35699:intron | 60.0% | |
| !! | GGTGGTGGTGCTGCCTTCTT+CGG | + | chr2.4:13690563-13690582 | None:intergenic | 60.0% |
| !! | TTCGCCGGCTTTGATGACGG+TGG | + | chr2.4:13690581-13690600 | None:intergenic | 60.0% |
| !! | GCCGGCTTTGATGACGGTGG+TGG | + | chr2.4:13690578-13690597 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 13690457 | 13690961 | 13690457 | ID=MS.gene35699 |
| chr2.4 | mRNA | 13690457 | 13690961 | 13690457 | ID=MS.gene35699.t1;Parent=MS.gene35699 |
| chr2.4 | exon | 13690959 | 13690961 | 13690959 | ID=MS.gene35699.t1.exon1;Parent=MS.gene35699.t1 |
| chr2.4 | CDS | 13690959 | 13690961 | 13690959 | ID=cds.MS.gene35699.t1;Parent=MS.gene35699.t1 |
| chr2.4 | exon | 13690655 | 13690861 | 13690655 | ID=MS.gene35699.t1.exon2;Parent=MS.gene35699.t1 |
| chr2.4 | CDS | 13690655 | 13690861 | 13690655 | ID=cds.MS.gene35699.t1;Parent=MS.gene35699.t1 |
| chr2.4 | exon | 13690457 | 13690570 | 13690457 | ID=MS.gene35699.t1.exon3;Parent=MS.gene35699.t1 |
| chr2.4 | CDS | 13690457 | 13690570 | 13690457 | ID=cds.MS.gene35699.t1;Parent=MS.gene35699.t1 |
| Gene Sequence |
| Protein sequence |
