AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36213

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36213.t1 MTR_2g103600 97.152 316 9 0 1 316 1 316 0.0 624
MS.gene36213.t1 MTR_4g021755 56.184 283 114 5 30 310 41 315 3.44e-101 303
MS.gene36213.t1 MTR_7g498280 56.357 291 118 4 15 304 20 302 1.85e-100 298
MS.gene36213.t1 MTR_7g061780 54.255 282 120 4 30 310 25 298 2.16e-95 286
MS.gene36213.t1 MTR_2g097210 55.636 275 114 3 30 302 43 311 2.53e-90 275
MS.gene36213.t1 MTR_2g097210 56.818 264 106 3 30 291 43 300 4.18e-89 268
MS.gene36213.t1 MTR_1g101310 48.366 306 138 3 3 304 5 294 3.04e-88 267
MS.gene36213.t1 MTR_3g073530 44.569 267 138 5 33 297 42 300 3.34e-55 184
MS.gene36213.t1 MTR_6g089790 68.421 57 16 1 191 247 1 55 5.77e-17 74.3
MS.gene36213.t1 MTR_2g088315 46.053 76 36 1 107 182 13 83 1.75e-15 70.9
MS.gene36213.t1 MTR_2g088290 30.435 207 90 8 51 252 1 158 1.90e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36213.t1 AT1G52580 60.797 301 109 7 1 299 1 294 2.54e-119 346
MS.gene36213.t1 AT2G29050 61.011 277 99 4 27 302 50 318 1.61e-108 321
MS.gene36213.t1 AT2G29050 55.932 295 93 5 27 292 50 336 1.53e-102 304
MS.gene36213.t1 AT1G63120 57.312 253 96 4 28 278 28 270 1.47e-97 291
MS.gene36213.t1 AT1G12750 51.786 280 126 4 29 306 16 288 1.08e-93 280
MS.gene36213.t1 AT1G12750 51.786 280 126 4 29 306 16 288 1.08e-93 280
MS.gene36213.t1 AT1G12750 51.786 280 126 4 29 306 16 288 1.08e-93 280
MS.gene36213.t1 AT1G12750 51.786 280 126 4 29 306 16 288 1.08e-93 280
MS.gene36213.t1 AT1G12750 51.786 280 126 4 29 306 16 288 1.08e-93 280
MS.gene36213.t1 AT5G07250 50.814 307 133 4 11 306 25 324 9.36e-91 274
MS.gene36213.t1 AT3G53780 50.554 271 122 4 30 300 63 321 2.10e-84 259
MS.gene36213.t1 AT3G53780 51.145 262 116 4 30 291 63 312 2.97e-83 254
MS.gene36213.t1 AT4G23070 51.971 279 123 5 27 304 25 293 1.02e-74 232
MS.gene36213.t1 AT1G12750 55.615 187 73 4 29 211 16 196 1.28e-65 205
MS.gene36213.t1 AT1G12750 56.284 183 72 3 29 209 16 192 1.37e-65 205
MS.gene36213.t1 AT3G53780 50.000 206 94 2 95 300 1 197 3.62e-64 204
MS.gene36213.t1 AT1G77860 41.132 265 136 5 33 290 48 299 2.35e-59 194
MS.gene36213.t1 AT1G77860 41.132 265 136 5 33 290 48 299 3.62e-59 194
MS.gene36213.t1 AT5G07250 53.398 206 80 3 11 205 25 225 7.22e-59 191
MS.gene36213.t1 AT1G25290 34.375 160 91 5 103 255 161 313 1.74e-14 73.6
MS.gene36213.t1 AT1G25290 34.416 154 92 4 103 255 161 306 2.73e-14 72.8
MS.gene36213.t1 AT5G38510 29.193 161 105 4 96 254 219 372 9.69e-12 65.9
MS.gene36213.t1 AT5G38510 29.193 161 105 4 96 254 219 372 9.69e-12 65.9
MS.gene36213.t1 AT5G38510 29.193 161 105 4 96 254 219 372 9.69e-12 65.9

Find 80 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTCTATATTTGCTTTC+TGG 0.112592 2.1:+2171561 MS.gene36213:CDS
TTGCCTTTGGCATCAATATT+TGG 0.121558 2.1:+2170669 MS.gene36213:CDS
GTTCGCCTTGAGAATGAATT+TGG 0.205071 2.1:+2171417 MS.gene36213:CDS
TCTCATCATTGGCCTAAATT+TGG 0.218525 2.1:+2172111 MS.gene36213:CDS
CTATATTTGCTTTCTGGCTT+TGG 0.237472 2.1:+2171567 MS.gene36213:CDS
TTGGATTTGGCAGTGAAAAT+TGG 0.260847 2.1:+2171540 MS.gene36213:intron
GCACCAACTGATACGGTATT+TGG 0.268428 2.1:-2171633 None:intergenic
TTGGACTATCTATTTAAATA+AGG 0.284285 2.1:+2171710 MS.gene36213:CDS
ATACACGAACATTGCAATAT+TGG 0.288693 2.1:-2170602 None:intergenic
TTCTGCAGGTTGAGGGTATT+AGG 0.315192 2.1:+2171273 MS.gene36213:intron
TAAATCCAACAGCCAAATTT+AGG 0.316777 2.1:-2172123 None:intergenic
TGCCTTTGGCATCAATATTT+GGG 0.350597 2.1:+2170670 MS.gene36213:CDS
AGAAAGTAGGCGGTGGTGGC+GGG 0.367689 2.1:-2170525 None:intergenic
TCAGTCATTACGCTACTTAT+CGG 0.370474 2.1:+2172322 MS.gene36213:CDS
AAACGTAAATCCAAGTATAA+AGG 0.381415 2.1:+2172274 MS.gene36213:CDS
TAGAAAGTAGGCGGTGGTGG+CGG 0.386984 2.1:-2170526 None:intergenic
CATATAGGAGGATTCCTATC+AGG 0.389877 2.1:+2172169 MS.gene36213:CDS
ACCGTATCAGTTGGTGCATC+GGG 0.403381 2.1:+2171639 MS.gene36213:CDS
GAATAAAAGACTAAACATAT+TGG 0.408803 2.1:-2171388 None:intergenic
TATTTGCTTTCTGGCTTTGG+TGG 0.424233 2.1:+2171570 MS.gene36213:CDS
ACTTGCATGTTTCTTCATGC+AGG 0.433987 2.1:+2171348 MS.gene36213:CDS
AGGCTATCAGTATTTCTTCC+TGG 0.441269 2.1:+2172294 MS.gene36213:CDS
ACTTTCAGTATTCGCTAAGA+AGG 0.444351 2.1:-2172701 None:intergenic
GTTTACATTCTGCAGGTTGA+GGG 0.447403 2.1:+2171266 MS.gene36213:intron
GAAAGTAGGCGGTGGTGGCG+GGG 0.449589 2.1:-2170524 None:intergenic
ATTTAAATAGATAGTCCAAT+TGG 0.450252 2.1:-2171706 None:intergenic
CAGTCATTACGCTACTTATC+GGG 0.457261 2.1:+2172323 MS.gene36213:CDS
ATAACGAGCCGCCTCAATAT+CGG 0.458145 2.1:-2170500 None:intergenic
ATACTGATAGCCTTTATACT+TGG 0.460587 2.1:-2172284 None:intergenic
TTGTTCACATAACCAAATTG+AGG 0.462978 2.1:-2172223 None:intergenic
ATTGGCCTAAATTTGGCTGT+TGG 0.471687 2.1:+2172118 MS.gene36213:CDS
AAAGTAGGCGGTGGTGGCGG+GGG 0.473559 2.1:-2170523 None:intergenic
TCATCTTCATTCAAATAGCC+AGG 0.474228 2.1:-2170646 None:intergenic
TTAGTGAGCTTCTAACCAAT+TGG 0.474947 2.1:+2171691 MS.gene36213:CDS
TACCGTATCAGTTGGTGCAT+CGG 0.475291 2.1:+2171638 MS.gene36213:CDS
TGAATGAAGATGATTGCCTT+TGG 0.479154 2.1:+2170656 MS.gene36213:CDS
ACAAGTCTACTTCTCATCAT+TGG 0.480296 2.1:+2172100 MS.gene36213:CDS
TTCAATGAAAACCCTCTCCT+TGG 0.483625 2.1:+2170712 MS.gene36213:CDS
ATGTAGAAAGTAGGCGGTGG+TGG 0.484370 2.1:-2170529 None:intergenic
TGGTGGGTGAAAATGAAGTA+TGG 0.484851 2.1:+2171316 MS.gene36213:CDS
TCATTTGACAGATATGCCTA+TGG 0.495016 2.1:+2172637 MS.gene36213:intron
TGTTCACATAACCAAATTGA+GGG 0.495056 2.1:-2172222 None:intergenic
CGCCAACACAATGGTTCTCA+TGG 0.513113 2.1:+2170557 MS.gene36213:CDS
GATTCTCACGTGCGGACAGA+AGG 0.516174 2.1:-2170736 None:intergenic
AAAATCCAAATTCATTCTCA+AGG 0.516279 2.1:-2171422 None:intergenic
GTGGACAATTCAGCTCATAT+AGG 0.523918 2.1:+2172154 MS.gene36213:CDS
AACACAATGGTTCTCATGGT+TGG 0.531112 2.1:+2170561 MS.gene36213:CDS
GGACAGAAGGGCCAAGGAGA+GGG 0.532109 2.1:-2170723 None:intergenic
CATGAGAACCATTGTGTTGG+CGG 0.546872 2.1:-2170556 None:intergenic
ACCCGATGCACCAACTGATA+CGG 0.550141 2.1:-2171640 None:intergenic
GTCGCCGTTCTCCGATATTG+AGG 0.551647 2.1:+2170489 MS.gene36213:CDS
GGTATTAGGAGCTCTTGAAA+AGG 0.553959 2.1:+2171287 MS.gene36213:CDS
ACGTGCGGACAGAAGGGCCA+AGG 0.568851 2.1:-2170729 None:intergenic
ATGTATATCAATGATTGCCC+TGG 0.571703 2.1:+2170628 MS.gene36213:CDS
AACCATGAGAACCATTGTGT+TGG 0.576399 2.1:-2170559 None:intergenic
TTCCCAAATATTGATGCCAA+AGG 0.579218 2.1:-2170672 None:intergenic
TCTTGAAAAGGACCTTGTGG+TGG 0.579909 2.1:+2171299 MS.gene36213:CDS
GTACAATGTAGCTAAGCCAT+AGG 0.586121 2.1:-2172653 None:intergenic
GGCGGTGGCATGTAGAAAGT+AGG 0.588387 2.1:-2170538 None:intergenic
GACAATTCAGCTCATATAGG+AGG 0.594629 2.1:+2172157 MS.gene36213:CDS
GGTTAGAAGCTCACTAAGCA+TGG 0.598778 2.1:-2171685 None:intergenic
GCGTAATGACTGACAAGACC+AGG 0.609130 2.1:-2172312 None:intergenic
ATTCTCACGTGCGGACAGAA+GGG 0.610517 2.1:-2170735 None:intergenic
CGGACAGAAGGGCCAAGGAG+AGG 0.611548 2.1:-2170724 None:intergenic
CATCTTCATTCAAATAGCCA+GGG 0.618324 2.1:-2170645 None:intergenic
GCCGCCTCAATATCGGAGAA+CGG 0.618640 2.1:-2170493 None:intergenic
GAGAACCATTGTGTTGGCGG+TGG 0.622152 2.1:-2170553 None:intergenic
GCACCAAATACCGTATCAGT+TGG 0.622766 2.1:+2171630 MS.gene36213:CDS
GGCTTAGCTACATTGTACAT+AGG 0.638607 2.1:+2172658 MS.gene36213:CDS
AGCTCTTGAAAAGGACCTTG+TGG 0.646384 2.1:+2171296 MS.gene36213:CDS
TGAGCTGAATTGTCCACATG+AGG 0.649223 2.1:-2172148 None:intergenic
GGCATGTAGAAAGTAGGCGG+TGG 0.649400 2.1:-2170532 None:intergenic
CTTGAAAAGGACCTTGTGGT+GGG 0.656591 2.1:+2171300 MS.gene36213:CDS
AACAACAAATATATACCACC+AGG 0.669042 2.1:+2172241 MS.gene36213:CDS
GCCGTTCTCCGATATTGAGG+CGG 0.669086 2.1:+2170492 MS.gene36213:CDS
ACATGCCACCGCCAACACAA+TGG 0.675117 2.1:+2170548 MS.gene36213:CDS
TGTTGGATTTATACCTCATG+TGG 0.677933 2.1:+2172135 MS.gene36213:CDS
GCATTAAAATAACAAAACCA+AGG 0.678293 2.1:-2172198 None:intergenic
GGTGGCATGTAGAAAGTAGG+CGG 0.681757 2.1:-2170535 None:intergenic
TGGGTGAAAATGAAGTATGG+CGG 0.777515 2.1:+2171319 MS.gene36213:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAAAAAAAAATACATA+GGG + chr2.1:2171767-2171786 MS.gene36213:intron 10.0%
!! TTACTTATTATATTTATTCT+TGG + chr2.1:2171521-2171540 MS.gene36213:intron 10.0%
!! AATTTGAATTTAATCTGTAT+TGG + chr2.1:2172584-2172603 MS.gene36213:intron 15.0%
!! ATTATATTTATTCTTGGATT+TGG + chr2.1:2171527-2171546 MS.gene36213:intron 15.0%
!! GAAAGAAAAAAAAAATACAT+AGG + chr2.1:2171766-2171785 MS.gene36213:intron 15.0%
!! AAAAAAAAAATACATAGGGT+GGG + chr2.1:2171771-2171790 MS.gene36213:intron 20.0%
!! ATTTAAATAGATAGTCCAAT+TGG - chr2.1:2171709-2171728 None:intergenic 20.0%
!! GAATAAAAGACTAAACATAT+TGG - chr2.1:2171391-2171410 None:intergenic 20.0%
!! TCAAAGTTAATCTTGTAAAT+TGG + chr2.1:2171863-2171882 MS.gene36213:intron 20.0%
!! TTGGACTATCTATTTAAATA+AGG + chr2.1:2171710-2171729 MS.gene36213:CDS 20.0%
!! TTTATGTTCAATCATAATGA+TGG + chr2.1:2172561-2172580 MS.gene36213:intron 20.0%
!!! ATGTTTAGTCTTTTATTCAT+AGG + chr2.1:2171393-2171412 MS.gene36213:CDS 20.0%
!!! CAAACGTTTTTAATAATTTG+AGG + chr2.1:2172470-2172489 MS.gene36213:intron 20.0%
!!! CATCATCATTTTAAATATCA+TGG + chr2.1:2170792-2170811 MS.gene36213:intron 20.0%
!!! CTCAATTTTTTGTTATATTG+CGG - chr2.1:2171017-2171036 None:intergenic 20.0%
!!! TTGTTATCTATTTTGCATTT+GGG + chr2.1:2171597-2171616 MS.gene36213:CDS 20.0%
!!! TTTGTTATCTATTTTGCATT+TGG + chr2.1:2171596-2171615 MS.gene36213:CDS 20.0%
! AAAATCCAAATTCATTCTCA+AGG - chr2.1:2171425-2171444 None:intergenic 25.0%
! AAACGTAAATCCAAGTATAA+AGG + chr2.1:2172274-2172293 MS.gene36213:CDS 25.0%
! ATTACATCAAAAGTTAAAGG+AGG - chr2.1:2172614-2172633 None:intergenic 25.0%
! CGCAATATAACAAAAAATTG+AGG + chr2.1:2171015-2171034 MS.gene36213:intron 25.0%
! GAAAAAAAAAATACATAGGG+TGG + chr2.1:2171770-2171789 MS.gene36213:intron 25.0%
! GCAATTACATCAAAAGTTAA+AGG - chr2.1:2172617-2172636 None:intergenic 25.0%
! GCATTAAAATAACAAAACCA+AGG - chr2.1:2172201-2172220 None:intergenic 25.0%
! GCTTTCTCATTAAAATATCA+TGG + chr2.1:2170838-2170857 MS.gene36213:intron 25.0%
! TCATAAGCTTGATTATAAAG+AGG + chr2.1:2171910-2171929 MS.gene36213:intron 25.0%
! TGCCGAAATATAAAAAATTG+CGG + chr2.1:2171087-2171106 MS.gene36213:intron 25.0%
!! ATATTGGCTAAAAAAATGAG+TGG - chr2.1:2170589-2170608 None:intergenic 25.0%
!! TTTTATATTTCGGCAAAACA+CGG - chr2.1:2171082-2171101 None:intergenic 25.0%
!!! ATCTTTTTACTTTTGAACCA+AGG + chr2.1:2172020-2172039 MS.gene36213:intron 25.0%
!!! CACCGCAATTTTTTATATTT+CGG - chr2.1:2171092-2171111 None:intergenic 25.0%
!!! TGAAATGATAGTGATTTGTT+TGG - chr2.1:2172416-2172435 None:intergenic 25.0%
!!! TTTGTCAATTGGTTGTTTTA+GGG + chr2.1:2171236-2171255 MS.gene36213:intron 25.0%
!!! TTTTGTCAATTGGTTGTTTT+AGG + chr2.1:2171235-2171254 MS.gene36213:intron 25.0%
AACAACAAATATATACCACC+AGG + chr2.1:2172241-2172260 MS.gene36213:CDS 30.0%
ATAATGTTCAACAATGTACC+TGG + chr2.1:2170816-2170835 MS.gene36213:intron 30.0%
ATACACGAACATTGCAATAT+TGG - chr2.1:2170605-2170624 None:intergenic 30.0%
ATACTGATAGCCTTTATACT+TGG - chr2.1:2172287-2172306 None:intergenic 30.0%
ATTTGAGGACTTTGATTGAT+TGG + chr2.1:2172485-2172504 MS.gene36213:intron 30.0%
CTTCTATTGTCTTACTTAGA+TGG + chr2.1:2171135-2171154 MS.gene36213:intron 30.0%
TAAATCCAACAGCCAAATTT+AGG - chr2.1:2172126-2172145 None:intergenic 30.0%
TCAAAAACACAGAGACAATT+TGG + chr2.1:2171950-2171969 MS.gene36213:intron 30.0%
TGTTCACATAACCAAATTGA+GGG - chr2.1:2172225-2172244 None:intergenic 30.0%
TTATTGTGTTTACATTCTGC+AGG + chr2.1:2171259-2171278 MS.gene36213:intron 30.0%
TTGTTCACATAACCAAATTG+AGG - chr2.1:2172226-2172245 None:intergenic 30.0%
!! AATGATAGTGATTTGTTTGG+CGG - chr2.1:2172413-2172432 None:intergenic 30.0%
!!! GATATTTTAATGAGAAAGCC+AGG - chr2.1:2170837-2170856 None:intergenic 30.0%
AACCAAGGAAAAATCCTGAT+AGG - chr2.1:2172186-2172205 None:intergenic 35.0%
ACAAGTCTACTTCTCATCAT+TGG + chr2.1:2172100-2172119 MS.gene36213:CDS 35.0%
ACTTTCAGTATTCGCTAAGA+AGG - chr2.1:2172704-2172723 None:intergenic 35.0%
ACTTTGAACTGTACTGTGTA+AGG - chr2.1:2171850-2171869 None:intergenic 35.0%
ATGTATATCAATGATTGCCC+TGG + chr2.1:2170628-2170647 MS.gene36213:CDS 35.0%
ATGTGAGAGAGAAATTGAGA+GGG - chr2.1:2170766-2170785 None:intergenic 35.0%
ATTATGTCAAATGCATGTCC+TGG + chr2.1:2170940-2170959 MS.gene36213:intron 35.0%
CATCTTCATTCAAATAGCCA+GGG - chr2.1:2170648-2170667 None:intergenic 35.0%
CTTTGAACTGTACTGTGTAA+GGG - chr2.1:2171849-2171868 None:intergenic 35.0%
GGAGTTCTATATTTGCTTTC+TGG + chr2.1:2171561-2171580 MS.gene36213:CDS 35.0%
TATGTGAGAGAGAAATTGAG+AGG - chr2.1:2170767-2170786 None:intergenic 35.0%
TCAGTCATTACGCTACTTAT+CGG + chr2.1:2172322-2172341 MS.gene36213:CDS 35.0%
TCATCTTCATTCAAATAGCC+AGG - chr2.1:2170649-2170668 None:intergenic 35.0%
TCATTTGACAGATATGCCTA+TGG + chr2.1:2172637-2172656 MS.gene36213:intron 35.0%
TGAATGAAGATGATTGCCTT+TGG + chr2.1:2170656-2170675 MS.gene36213:CDS 35.0%
TGCCTTTGGCATCAATATTT+GGG + chr2.1:2170670-2170689 MS.gene36213:CDS 35.0%
TGTTGGATTTATACCTCATG+TGG + chr2.1:2172135-2172154 MS.gene36213:CDS 35.0%
TTAGTGAGCTTCTAACCAAT+TGG + chr2.1:2171691-2171710 MS.gene36213:CDS 35.0%
TTCCCAAATATTGATGCCAA+AGG - chr2.1:2170675-2170694 None:intergenic 35.0%
TTGCCTTTGGCATCAATATT+TGG + chr2.1:2170669-2170688 MS.gene36213:CDS 35.0%
TTGGATTTGGCAGTGAAAAT+TGG + chr2.1:2171540-2171559 MS.gene36213:intron 35.0%
TTTGAACTGTACTGTGTAAG+GGG - chr2.1:2171848-2171867 None:intergenic 35.0%
! ACAGTGTCTCTTTTGTCAAT+TGG + chr2.1:2171225-2171244 MS.gene36213:intron 35.0%
! TCTCATCATTGGCCTAAATT+TGG + chr2.1:2172111-2172130 MS.gene36213:CDS 35.0%
! TTACGTTTTGCATCATATCC+TGG - chr2.1:2172262-2172281 None:intergenic 35.0%
! TTCCTATCAGGATTTTTCCT+TGG + chr2.1:2172181-2172200 MS.gene36213:CDS 35.0%
!! ATTTTAATGCGCCCTCAATT+TGG + chr2.1:2172211-2172230 MS.gene36213:CDS 35.0%
!! CTATATTTGCTTTCTGGCTT+TGG + chr2.1:2171567-2171586 MS.gene36213:CDS 35.0%
!!! ACATTTTAAAACCCCATGCA+TGG - chr2.1:2170973-2170992 None:intergenic 35.0%
!!! ATGCATGGGGTTTTAAAATG+TGG + chr2.1:2170972-2170991 MS.gene36213:intron 35.0%
AACACAATGGTTCTCATGGT+TGG + chr2.1:2170561-2170580 MS.gene36213:CDS 40.0%
AACCATGAGAACCATTGTGT+TGG - chr2.1:2170562-2170581 None:intergenic 40.0%
ACTTGCATGTTTCTTCATGC+AGG + chr2.1:2171348-2171367 MS.gene36213:CDS 40.0%
AGGCTATCAGTATTTCTTCC+TGG + chr2.1:2172294-2172313 MS.gene36213:CDS 40.0%
ATCTGTTAGAACAACAGCCT+TGG - chr2.1:2172040-2172059 None:intergenic 40.0%
CAGTCATTACGCTACTTATC+GGG + chr2.1:2172323-2172342 MS.gene36213:CDS 40.0%
CATATAGGAGGATTCCTATC+AGG + chr2.1:2172169-2172188 MS.gene36213:CDS 40.0%
CCTACAATACATGCATGCAT+AGG + chr2.1:2171187-2171206 MS.gene36213:intron 40.0%
CCTATGCATGCATGTATTGT+AGG - chr2.1:2171190-2171209 None:intergenic 40.0%
GACAATTCAGCTCATATAGG+AGG + chr2.1:2172157-2172176 MS.gene36213:CDS 40.0%
GCACACTGTAGAAGTAAACA+TGG - chr2.1:2172073-2172092 None:intergenic 40.0%
GGCTTAGCTACATTGTACAT+AGG + chr2.1:2172658-2172677 MS.gene36213:CDS 40.0%
GGTATTAGGAGCTCTTGAAA+AGG + chr2.1:2171287-2171306 MS.gene36213:CDS 40.0%
GTACAATGTAGCTAAGCCAT+AGG - chr2.1:2172656-2172675 None:intergenic 40.0%
GTGGACAATTCAGCTCATAT+AGG + chr2.1:2172154-2172173 MS.gene36213:CDS 40.0%
GTTCGCCTTGAGAATGAATT+TGG + chr2.1:2171417-2171436 MS.gene36213:CDS 40.0%
TGGGTGAAAATGAAGTATGG+CGG + chr2.1:2171319-2171338 MS.gene36213:CDS 40.0%
TGTTTACATTCTGCAGGTTG+AGG + chr2.1:2171265-2171284 MS.gene36213:intron 40.0%
TTCAATGAAAACCCTCTCCT+TGG + chr2.1:2170712-2170731 MS.gene36213:CDS 40.0%
! AGGAGAGGGTTTTCATTGAA+AGG - chr2.1:2170712-2170731 None:intergenic 40.0%
! ATTGGCCTAAATTTGGCTGT+TGG + chr2.1:2172118-2172137 MS.gene36213:CDS 40.0%
! GTTTACATTCTGCAGGTTGA+GGG + chr2.1:2171266-2171285 MS.gene36213:intron 40.0%
! TGGTGGGTGAAAATGAAGTA+TGG + chr2.1:2171316-2171335 MS.gene36213:CDS 40.0%
!! TATTTGCTTTCTGGCTTTGG+TGG + chr2.1:2171570-2171589 MS.gene36213:CDS 40.0%
AGCTCTTGAAAAGGACCTTG+TGG + chr2.1:2171296-2171315 MS.gene36213:CDS 45.0%
ATAACGAGCCGCCTCAATAT+CGG - chr2.1:2170503-2170522 None:intergenic 45.0%
CATGAGAACCATTGTGTTGG+CGG - chr2.1:2170559-2170578 None:intergenic 45.0%
CCTGGATTACTTCCATGCAT+GGG + chr2.1:2170958-2170977 MS.gene36213:intron 45.0%
CTGGATTACTTCCATGCATG+GGG + chr2.1:2170959-2170978 MS.gene36213:intron 45.0%
CTTGAAAAGGACCTTGTGGT+GGG + chr2.1:2171300-2171319 MS.gene36213:CDS 45.0%
GCACCAAATACCGTATCAGT+TGG + chr2.1:2171630-2171649 MS.gene36213:CDS 45.0%
GCACCAACTGATACGGTATT+TGG - chr2.1:2171636-2171655 None:intergenic 45.0%
GGTTAGAAGCTCACTAAGCA+TGG - chr2.1:2171688-2171707 None:intergenic 45.0%
TCCTGGATTACTTCCATGCA+TGG + chr2.1:2170957-2170976 MS.gene36213:intron 45.0%
TCTTGAAAAGGACCTTGTGG+TGG + chr2.1:2171299-2171318 MS.gene36213:CDS 45.0%
TGAGCTGAATTGTCCACATG+AGG - chr2.1:2172151-2172170 None:intergenic 45.0%
! CGTTTTGCATCATATCCTGG+TGG - chr2.1:2172259-2172278 None:intergenic 45.0%
! CTTCATTTTCACCCACCACA+AGG - chr2.1:2171314-2171333 None:intergenic 45.0%
! TACCGTATCAGTTGGTGCAT+CGG + chr2.1:2171638-2171657 MS.gene36213:CDS 45.0%
! TCACGGAACACTGTCCTTTT+TGG - chr2.1:2171803-2171822 None:intergenic 45.0%
!! CTCTGTGTTTTTGACCGTAG+AGG - chr2.1:2171944-2171963 None:intergenic 45.0%
!! GGTGCACTTTTTGGACTTCT+GGG + chr2.1:2171660-2171679 MS.gene36213:CDS 45.0%
!! TTCTGCAGGTTGAGGGTATT+AGG + chr2.1:2171273-2171292 MS.gene36213:intron 45.0%
AAGGACAGTGTTCCGTGACT+CGG + chr2.1:2171805-2171824 MS.gene36213:intron 50.0%
ACCCGATGCACCAACTGATA+CGG - chr2.1:2171643-2171662 None:intergenic 50.0%
AGGGAGAAGATTCTCACGTG+CGG - chr2.1:2170747-2170766 None:intergenic 50.0%
ATGTAGAAAGTAGGCGGTGG+TGG - chr2.1:2170532-2170551 None:intergenic 50.0%
ATTCTCACGTGCGGACAGAA+GGG - chr2.1:2170738-2170757 None:intergenic 50.0%
CCCATGCATGGAAGTAATCC+AGG - chr2.1:2170961-2170980 None:intergenic 50.0%
CGCCAACACAATGGTTCTCA+TGG + chr2.1:2170557-2170576 MS.gene36213:CDS 50.0%
GCGTAATGACTGACAAGACC+AGG - chr2.1:2172315-2172334 None:intergenic 50.0%
GGTGGCATGTAGAAAGTAGG+CGG - chr2.1:2170538-2170557 None:intergenic 50.0%
! ACCGTATCAGTTGGTGCATC+GGG + chr2.1:2171639-2171658 MS.gene36213:CDS 50.0%
!! AAGAGGCGTTGTAGCCTCTA+CGG + chr2.1:2171927-2171946 MS.gene36213:intron 50.0%
!! GGGTGCACTTTTTGGACTTC+TGG + chr2.1:2171659-2171678 MS.gene36213:CDS 50.0%
ACATGCCACCGCCAACACAA+TGG + chr2.1:2170548-2170567 MS.gene36213:CDS 55.0%
AGGACAGTGTTCCGTGACTC+GGG + chr2.1:2171806-2171825 MS.gene36213:intron 55.0%
AGGGTGGGTGTGTGCCAAAA+AGG + chr2.1:2171786-2171805 MS.gene36213:intron 55.0%
GATTCTCACGTGCGGACAGA+AGG - chr2.1:2170739-2170758 None:intergenic 55.0%
GCCGCCTCAATATCGGAGAA+CGG - chr2.1:2170496-2170515 None:intergenic 55.0%
GCCGTTCTCCGATATTGAGG+CGG + chr2.1:2170492-2170511 MS.gene36213:CDS 55.0%
GGCATGTAGAAAGTAGGCGG+TGG - chr2.1:2170535-2170554 None:intergenic 55.0%
GGCGGTGGCATGTAGAAAGT+AGG - chr2.1:2170541-2170560 None:intergenic 55.0%
GTCGCCGTTCTCCGATATTG+AGG + chr2.1:2170489-2170508 MS.gene36213:CDS 55.0%
! GAGAACCATTGTGTTGGCGG+TGG - chr2.1:2170556-2170575 None:intergenic 55.0%
! GGTGCATCGGGTGCACTTTT+TGG + chr2.1:2171651-2171670 MS.gene36213:CDS 55.0%
! TAGAAAGTAGGCGGTGGTGG+CGG - chr2.1:2170529-2170548 None:intergenic 55.0%
GGACAGAAGGGCCAAGGAGA+GGG - chr2.1:2170726-2170745 None:intergenic 60.0%
GGACAGTGTTCCGTGACTCG+GGG + chr2.1:2171807-2171826 MS.gene36213:intron 60.0%
! AGAAAGTAGGCGGTGGTGGC+GGG - chr2.1:2170528-2170547 None:intergenic 60.0%
ACGTGCGGACAGAAGGGCCA+AGG - chr2.1:2170732-2170751 None:intergenic 65.0%
CGGACAGAAGGGCCAAGGAG+AGG - chr2.1:2170727-2170746 None:intergenic 65.0%
! GAAAGTAGGCGGTGGTGGCG+GGG - chr2.1:2170527-2170546 None:intergenic 65.0%
! GTTCCGTGACTCGGGGTGCA+GGG + chr2.1:2171814-2171833 MS.gene36213:intron 65.0%
! TGTTCCGTGACTCGGGGTGC+AGG + chr2.1:2171813-2171832 MS.gene36213:intron 65.0%
! TTCCGTGACTCGGGGTGCAG+GGG + chr2.1:2171815-2171834 MS.gene36213:intron 65.0%
!! AAAGTAGGCGGTGGTGGCGG+GGG - chr2.1:2170526-2170545 None:intergenic 65.0%
! TCCGTGACTCGGGGTGCAGG+GGG + chr2.1:2171816-2171835 MS.gene36213:intron 70.0%
CCCCCCTGCACCCCGAGTCA+CGG - chr2.1:2171820-2171839 None:intergenic 75.0%
! CCGTGACTCGGGGTGCAGGG+GGG + chr2.1:2171817-2171836 MS.gene36213:intron 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 2170478 2172726 2170478 ID=MS.gene36213
chr2.1 mRNA 2170478 2172726 2170478 ID=MS.gene36213.t1;Parent=MS.gene36213
chr2.1 exon 2170478 2170749 2170478 ID=MS.gene36213.t1.exon1;Parent=MS.gene36213.t1
chr2.1 CDS 2170478 2170749 2170478 ID=cds.MS.gene36213.t1;Parent=MS.gene36213.t1
chr2.1 exon 2171281 2171444 2171281 ID=MS.gene36213.t1.exon2;Parent=MS.gene36213.t1
chr2.1 CDS 2171281 2171444 2171281 ID=cds.MS.gene36213.t1;Parent=MS.gene36213.t1
chr2.1 exon 2171553 2171731 2171553 ID=MS.gene36213.t1.exon3;Parent=MS.gene36213.t1
chr2.1 CDS 2171553 2171731 2171553 ID=cds.MS.gene36213.t1;Parent=MS.gene36213.t1
chr2.1 exon 2172088 2172344 2172088 ID=MS.gene36213.t1.exon4;Parent=MS.gene36213.t1
chr2.1 CDS 2172088 2172344 2172088 ID=cds.MS.gene36213.t1;Parent=MS.gene36213.t1
chr2.1 exon 2172648 2172726 2172648 ID=MS.gene36213.t1.exon5;Parent=MS.gene36213.t1
chr2.1 CDS 2172648 2172726 2172648 ID=cds.MS.gene36213.t1;Parent=MS.gene36213.t1
Gene Sequence

>MS.gene36213

ATGGGGAAAAAGTCGCCGTTCTCCGATATTGAGGCGGCTCGTTATCCCCCGCCACCACCGCCTACTTTCTACATGCCACCGCCAACACAATGGTTCTCATGGTTGGTGCCACTCATTTTTTTAGCCAATATTGCAATGTTCGTGTATAGCATGTATATCAATGATTGCCCTGGCTATTTGAATGAAGATGATTGCCTTTGGCATCAATATTTGGGAAGATTTTCTTTTCAACCTTTCAATGAAAACCCTCTCCTTGGCCCTTCTGTCCGCACGTGAGAATCTTCTCCCTCTCAATTTCTCTCTCACATAATCATCATCATCATTTTAAATATCATGGTATAATGTTCAACAATGTACCTGGCTTTCTCATTAAAATATCATGGTATATTGCATTTTCATATTGAAAAGTTAAGATTTTTGAAATGATGAACATTTAGAATTTTTGAATCATCAATTTGAATGATTATGTCAAATGCATGTCCTGGATTACTTCCATGCATGGGGTTTTAAAATGTGGTTTGCAACAGCAATTGCAGCCGCAATATAACAAAAAATTGAGGTCTCTGCAATGACGTCGCAGCTACAATATCAACTGAATCGACCGTGTTTTGCCGAAATATAAAAAATTGCGGTGCGCAACAGCAATTTAAAACTATGCTTCTATTGTCTTACTTAGATGGTATTCAACATATGACATAAAATGAAAATGCCTACAATACATGCATGCATAGGAAGAAGAAGAAAAAAACAGTGTCTCTTTTGTCAATTGGTTGTTTTAGGGTTATTGTGTTTACATTCTGCAGGTTGAGGGTATTAGGAGCTCTTGAAAAGGACCTTGTGGTGGGTGAAAATGAAGTATGGCGGTTCTTTACTTGCATGTTTCTTCATGCAGGAGTTATACATTTACTTGCCAATATGTTTAGTCTTTTATTCATAGGAGTTCGCCTTGAGAATGAATTTGGATTTTGTAAGCATATCTCAATTTATATTGAGTTTTTTTTTTTCAACAAGTTGAAACTCTTTTCTATCTTTGTTTAGTTGATTTACTTATTATATTTATTCTTGGATTTGGCAGTGAAAATTGGAGTTCTATATTTGCTTTCTGGCTTTGGTGGAAGTTTGTTATCTATTTTGCATTTGGGAGATGCTAAAGCACCAAATACCGTATCAGTTGGTGCATCGGGTGCACTTTTTGGACTTCTGGGAGCCATGCTTAGTGAGCTTCTAACCAATTGGACTATCTATTTAAATAAGGTACTTTATGATGATATATTTTGTTGTCTTGAAAGAAAGAAAAAAAAAATACATAGGGTGGGTGTGTGCCAAAAAGGACAGTGTTCCGTGACTCGGGGTGCAGGGGGGAGAAACCCCTTACACAGTACAGTTCAAAGTTAATCTTGTAAATTGGACTGTAGCATTTAAGTACTGTAAATCATAAGCTTGATTATAAAGAGGCGTTGTAGCCTCTACGGTCAAAAACACAGAGACAATTTGGTCATATCAAGTGATCAAATATGTGTTTTCTCTACTTGCATCTTTTGTATCTTTTTACTTTTGAACCAAGGCTGTTGTTCTAACAGATAGATTTGAAACCATGTTTACTTCTACAGTGTGCAGCTCTAACAAGTCTACTTCTCATCATTGGCCTAAATTTGGCTGTTGGATTTATACCTCATGTGGACAATTCAGCTCATATAGGAGGATTCCTATCAGGATTTTTCCTTGGTTTTGTTATTTTAATGCGCCCTCAATTTGGTTATGTGAACAACAAATATATACCACCAGGATATGATGCAAAACGTAAATCCAAGTATAAAGGCTATCAGTATTTCTTCCTGGTCTTGTCAGTCATTACGCTACTTATCGGGTAAGTAATTCATGATTTTCTGAGTTTAATTTCGATACAGTGTTGTCAATGTAAACAAATTTACACCGCCAAACAAATCACTATCATTTCATGTGTGTGACTTTTTGAATACTTATGATTAAAGTCAAACGTTTTTAATAATTTGAGGACTTTGATTGATTGGCAATGTAAAAAGTATCAACAGTGACAATGTATATAAATTTAATCATTTCTTTTTTTATGTTCAATCATAATGATGGAATTTGAATTTAATCTGTATTGGTTAACCTCCTTTAACTTTTGATGTAATTGCTCATTTGACAGATATGCCTATGGCTTAGCTACATTGTACATAGGAGAATCAAATGATGTATTTTCCTTCTTAGCGAATACTGAAAGTTAA
Protein sequence

>MS.gene36213.t1

MGKKSPFSDIEAARYPPPPPPTFYMPPPTQWFSWLVPLIFLANIAMFVYSMYINDCPGYLNEDDCLWHQYLGRFSFQPFNENPLLGPSVRTLRVLGALEKDLVVGENEVWRFFTCMFLHAGVIHLLANMFSLLFIGVRLENEFGFLKIGVLYLLSGFGGSLLSILHLGDAKAPNTVSVGASGALFGLLGAMLSELLTNWTIYLNKCAALTSLLLIIGLNLAVGFIPHVDNSAHIGGFLSGFFLGFVILMRPQFGYVNNKYIPPGYDAKRKSKYKGYQYFFLVLSVITLLIGYAYGLATLYIGESNDVFSFLANTES