AlfalfaGEDB | MS.gene36957

M. sativa cultivar XinJiangDaYe / MS.gene36957

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0008270 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0045893 GO:0045935 GO:0046872 GO:0046914 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36957.t1	XP_003627002.1	99.7	342	1	0	1	342	1	342	4.10E-167	597.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36957.t1	O82632	50.0	272	96	11	33	290	16	261	3.1e-54	213.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36957.t1	G7LFY2	99.7	342	1	0	1	342	1	342	3.0e-167	597.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene36957.t1	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049298	MS.gene36957	0.841698	4.06E-58	-1.69E-46
MS.gene050710	MS.gene36957	0.815702	8.07E-52	-1.69E-46
MS.gene050875	MS.gene36957	0.802136	6.47E-49	-1.69E-46
MS.gene051914	MS.gene36957	0.811469	6.89E-51	-1.69E-46
MS.gene052209	MS.gene36957	0.809611	1.74E-50	-1.69E-46
MS.gene054941	MS.gene36957	0.854736	1.01E-61	-1.69E-46
MS.gene056387	MS.gene36957	-0.801652	8.14E-49	-1.69E-46
MS.gene05652	MS.gene36957	0.840012	1.12E-57	-1.69E-46
MS.gene057367	MS.gene36957	0.810962	8.88E-51	-1.69E-46
MS.gene057669	MS.gene36957	0.855886	4.67E-62	-1.69E-46
MS.gene057717	MS.gene36957	0.82994	3.88E-55	-1.69E-46
MS.gene058128	MS.gene36957	0.816124	6.50E-52	-1.69E-46
MS.gene05926	MS.gene36957	0.838391	2.96E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36957.t1	MTR_8g013950	99.708	342	1	0	1	342	1	342	0.0	713
MS.gene36957.t1	MTR_1g006650	46.291	337	134	11	35	342	26	344	2.72e-82	253
MS.gene36957.t1	MTR_3g109760	44.959	367	128	11	1	342	1	318	2.80e-81	250
MS.gene36957.t1	MTR_8g076340	39.362	282	119	7	31	295	41	287	5.36e-48	163
MS.gene36957.t1	MTR_4g071590	38.489	278	120	10	33	295	39	280	6.12e-43	150
MS.gene36957.t1	MTR_4g471590	38.489	278	120	10	33	295	39	280	6.12e-43	150
MS.gene36957.t1	MTR_5g021340	39.916	238	81	6	102	295	150	369	2.50e-40	146
MS.gene36957.t1	MTR_3g117380	37.319	276	130	6	33	295	53	298	6.88e-40	143
MS.gene36957.t1	MTR_6g032830	38.889	234	77	10	115	292	35	258	1.57e-35	130
MS.gene36957.t1	MTR_1g046680	58.824	119	44	3	179	295	126	241	5.99e-35	128
MS.gene36957.t1	MTR_1g094930	81.538	65	12	0	226	290	147	211	2.33e-33	124
MS.gene36957.t1	MTR_5g007600	76.389	72	17	0	225	296	258	329	2.42e-33	126
MS.gene36957.t1	MTR_5g007600	40.426	188	89	5	112	289	46	220	1.10e-29	116
MS.gene36957.t1	MTR_1g094930	72.973	74	20	0	226	299	242	315	8.55e-33	124
MS.gene36957.t1	MTR_7g085410	75.385	65	16	0	226	290	188	252	4.98e-31	118
MS.gene36957.t1	MTR_4g058910	52.252	111	40	1	212	322	300	397	1.24e-29	117
MS.gene36957.t1	MTR_1g116010	75.385	65	16	0	226	290	164	228	1.81e-29	114
MS.gene36957.t1	MTR_2g094600	75.385	65	16	0	226	290	216	280	2.34e-29	115
MS.gene36957.t1	MTR_7g446110	53.947	76	34	1	213	287	173	248	4.03e-21	91.3
MS.gene36957.t1	MTR_7g445950	32.044	181	94	5	107	287	30	181	7.51e-19	83.6
MS.gene36957.t1	MTR_7g446070	30.939	181	108	4	107	287	30	193	1.15e-18	83.2
MS.gene36957.t1	MTR_7g445980	60.000	60	23	1	230	288	37	96	8.87e-18	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36957.t1	AT4G32890	48.308	325	121	13	33	342	16	308	2.48e-82	253
MS.gene36957.t1	AT5G25830	45.278	360	103	15	35	342	14	331	5.84e-72	227
MS.gene36957.t1	AT3G60530	48.792	207	58	8	101	298	63	230	8.56e-49	164
MS.gene36957.t1	AT2G45050	44.495	218	68	5	104	300	68	253	6.83e-48	162
MS.gene36957.t1	AT5G66320	41.277	235	79	6	102	294	100	317	6.18e-41	146
MS.gene36957.t1	AT5G66320	41.277	235	79	6	102	294	100	317	6.18e-41	146
MS.gene36957.t1	AT4G36240	38.745	271	104	9	35	293	11	231	5.84e-40	141
MS.gene36957.t1	AT4G34680	44.503	191	70	5	107	295	93	249	7.18e-39	139
MS.gene36957.t1	AT4G34680	44.503	191	70	5	107	295	93	249	7.18e-39	139
MS.gene36957.t1	AT1G08010	76.056	71	17	0	226	296	220	290	9.81e-34	126
MS.gene36957.t1	AT1G08010	76.056	71	17	0	226	296	220	290	9.81e-34	126
MS.gene36957.t1	AT1G08010	76.056	71	17	0	226	296	220	290	9.81e-34	126
MS.gene36957.t1	AT3G24050	77.941	68	15	0	225	292	193	260	1.55e-33	125
MS.gene36957.t1	AT3G54810	61.702	94	35	1	226	319	229	321	1.66e-32	124
MS.gene36957.t1	AT3G54810	61.702	94	35	1	226	319	229	321	1.66e-32	124
MS.gene36957.t1	AT3G51080	59.783	92	37	0	226	317	221	312	3.97e-31	120
MS.gene36957.t1	AT1G08000	74.627	67	17	0	226	292	218	284	1.15e-30	118
MS.gene36957.t1	AT1G08000	74.627	67	17	0	226	292	218	284	1.15e-30	118
MS.gene36957.t1	AT3G45170	57.143	77	33	0	218	294	107	183	5.22e-26	103
MS.gene36957.t1	AT2G28340	68.254	63	20	0	228	290	193	255	5.02e-25	102
MS.gene36957.t1	AT3G06740	33.684	95	38	2	226	305	41	125	6.87e-11	60.5

Find 88 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCCTGGTAGAGGTGTTTC+TGG	0.198536	8.4:-77012124	None:intergenic
GGAAGAGTCGGTGGAATTTC+CGG	0.199866	8.4:-77011677	None:intergenic
CTCTTTACAGTATGATGATA+TGG	0.231727	8.4:+77011916	MS.gene36957:intron
GGAGGCACAATTGTTCCTTA+TGG	0.277726	8.4:+77011757	MS.gene36957:CDS
ACACGCATTGCAGAGTGTTT+TGG	0.286863	8.4:-77012338	None:intergenic
CTTCCAGAACATTCCACATT+TGG	0.294560	8.4:-77012256	None:intergenic
GAATTCTCATAGGAAAGTTT+TGG	0.321671	8.4:+77012441	MS.gene36957:CDS
GACGCTTCTCCGGCGAGAAT+TGG	0.338370	8.4:-77011549	None:intergenic
AGGCGCGTGGACCAGTCTCC+TGG	0.390976	8.4:-77012175	None:intergenic
GTATGATGATATGGCGGAAC+TGG	0.396674	8.4:+77011925	MS.gene36957:intron
GATCCAAATGTGGAATGTTC+TGG	0.398960	8.4:+77012253	MS.gene36957:CDS
AGACATAATCGCGTCGGCAT+TGG	0.413357	8.4:-77011629	None:intergenic
AGCTGTAACTCCTCTGGCTC+TGG	0.416860	8.4:+77011718	MS.gene36957:CDS
CACGCGCTGCTCCAGGAGAC+TGG	0.453901	8.4:+77012164	MS.gene36957:CDS
ATCAATTATCATCATCATTG+TGG	0.463155	8.4:+77012583	MS.gene36957:CDS
TACTTTGTCGGTGGCTACTA+CGG	0.465894	8.4:+77011514	MS.gene36957:CDS
AAGACGCCGCAGTGGAGAAC+AGG	0.467796	8.4:+77012304	MS.gene36957:CDS
TGGTCACCGTGACGCTTCTC+CGG	0.476812	8.4:-77011559	None:intergenic
CGAAGCATTCGAATTCTCAT+AGG	0.477067	8.4:+77012431	MS.gene36957:CDS
AAACGATCACGCGCTGCTCC+AGG	0.479220	8.4:+77012157	MS.gene36957:CDS
TCCGGTGATGTTCAACTCAC+CGG	0.479652	8.4:+77011784	MS.gene36957:CDS
TCAATTATCATCATCATTGT+GGG	0.480828	8.4:+77012584	MS.gene36957:CDS
GAAGCACAAGATTACTTTGT+CGG	0.482794	8.4:+77011502	MS.gene36957:CDS
CACCGGAGAACTCTGTGTTC+CGG	0.489493	8.4:+77011801	MS.gene36957:CDS
AAAACATTGCAACTTCTCTC+CGG	0.498282	8.4:+77011998	MS.gene36957:CDS
CGGTTTAACAGCACCGGCGC+CGG	0.503197	8.4:-77012017	None:intergenic
GATGAAGATTCCGGTGTTTG+CGG	0.503689	8.4:-77012037	None:intergenic
TAAGTCCGGTAGGTTGGTGC+CGG	0.508279	8.4:+77012372	MS.gene36957:CDS
TTGCAACTTCTCTCCGGCGC+CGG	0.510336	8.4:+77012004	MS.gene36957:CDS
TCTGGTGGTGATAACCGCTT+TGG	0.512040	8.4:+77011736	MS.gene36957:CDS
GAAATCAAGTAAATCATCAA+TGG	0.517205	8.4:-77011605	None:intergenic
GTTGACAGCTGTAACTCCTC+TGG	0.518284	8.4:+77011712	MS.gene36957:CDS
GGTGGTGATAACCGCTTTGG+AGG	0.525558	8.4:+77011739	MS.gene36957:CDS
TGCTTCGCCGATACAAAAGT+TGG	0.526129	8.4:-77012415	None:intergenic
CTCGCCGCCGGTCGATACTC+CGG	0.528513	8.4:-77012391	None:intergenic
TCCGGTGAGTTGAACATCAC+CGG	0.532904	8.4:-77011785	None:intergenic
GTCGGTGGCTACTACGGTGC+CGG	0.534317	8.4:+77011520	MS.gene36957:CDS
CTGGTAGAGGTGTTTCTGGA+CGG	0.544153	8.4:-77012120	None:intergenic
CGTCCAGAAACACCTCTACC+AGG	0.545940	8.4:+77012121	MS.gene36957:CDS
GGAATGGCTTTCGAATTTCG+TGG	0.546230	8.4:+77011946	MS.gene36957:CDS
TCGGTGGAAGATGAAGATTC+CGG	0.552072	8.4:-77012046	None:intergenic
TTCTTCGATAACAATGTCGC+CGG	0.552150	8.4:+77011658	MS.gene36957:CDS
ATACTCCGGCACCAACCTAC+CGG	0.552745	8.4:-77012377	None:intergenic
GAGGTTTAAGTCCGGTAGGT+TGG	0.552850	8.4:+77012366	MS.gene36957:CDS
GCTGTCAACAGCGGTGACAT+TGG	0.554444	8.4:-77011698	None:intergenic
GTTTCGTCGGTGGAGAATGA+CGG	0.557773	8.4:-77012076	None:intergenic
TACCGGAACACAGAGTTCTC+CGG	0.562215	8.4:-77011803	None:intergenic
GCGCGTCGGGGAGATGAAGT+AGG	0.565923	8.4:-77012195	None:intergenic
TGTAACTCCTCTGGCTCTGG+TGG	0.567338	8.4:+77011721	MS.gene36957:CDS
GCAGTGGAGAACAGGACCGA+TGG	0.568184	8.4:+77012312	MS.gene36957:CDS
AGCGGTGACATTGGAAGAGT+CGG	0.570500	8.4:-77011689	None:intergenic
TGCTGTTAAACCGCAAACAC+CGG	0.571815	8.4:+77012027	MS.gene36957:CDS
TGTTTGCGGTTTAACAGCAC+CGG	0.574755	8.4:-77012023	None:intergenic
ATGATATGGCGGAACTGGAA+TGG	0.580000	8.4:+77011930	MS.gene36957:CDS
ACAAAAGTTGGACTCGCCGC+CGG	0.580278	8.4:-77012403	None:intergenic
CAGTGGAGAACAGGACCGAT+GGG	0.585257	8.4:+77012313	MS.gene36957:CDS
TAGATAACATGTCTAAACTC+TGG	0.596187	8.4:-77012607	None:intergenic
GTTGGTGCCGGAGTATCGAC+CGG	0.600084	8.4:+77012384	MS.gene36957:CDS
GTTATCACCACCAGAGCCAG+AGG	0.602646	8.4:-77011728	None:intergenic
AGTAAATCATCAATGGCGAA+AGG	0.614239	8.4:-77011598	None:intergenic
TTCTCGCCGGAGAAGCGTCA+CGG	0.616797	8.4:+77011553	MS.gene36957:CDS
TCAATGGCGAAAGGCTCACA+CGG	0.620341	8.4:-77011589	None:intergenic
CGGCGAAAACCAATTCTCGC+CGG	0.631060	8.4:+77011540	MS.gene36957:CDS
GCCGACGCGATTATGTCTGA+CGG	0.636094	8.4:+77011634	MS.gene36957:CDS
GCACAAGATTACTTTGTCGG+TGG	0.638723	8.4:+77011505	MS.gene36957:CDS
GTTACGAGCAGTTTCGTCGG+TGG	0.641102	8.4:-77012086	None:intergenic
TGTGGTGTGAGGTTTAAGTC+CGG	0.641447	8.4:+77012358	MS.gene36957:CDS
GTGTGAGGTTTAAGTCCGGT+AGG	0.641902	8.4:+77012362	MS.gene36957:CDS
GGGGAGATGAAGTAGGCGCG+TGG	0.645736	8.4:-77012188	None:intergenic
ACCGTCAGACATAATCGCGT+CGG	0.646289	8.4:-77011635	None:intergenic
GAATGACGGAAGTGTGTCGG+TGG	0.649487	8.4:-77012062	None:intergenic
TCTGCAATGCGTGTGGTGTG+AGG	0.651426	8.4:+77012347	MS.gene36957:CDS
AAAATGTCTCCACTGCGGCA+CGG	0.653917	8.4:+77012279	MS.gene36957:CDS
AGCGTTACGAGCAGTTTCGT+CGG	0.655792	8.4:-77012089	None:intergenic
AAAGCGTGAGGATCCAAATG+TGG	0.658111	8.4:+77012243	MS.gene36957:CDS
GCGTCTTATCCGTGCCGCAG+TGG	0.667437	8.4:-77012288	None:intergenic
GGAGAATGACGGAAGTGTGT+CGG	0.671284	8.4:-77012065	None:intergenic
GGTGACATTGGAAGAGTCGG+TGG	0.674783	8.4:-77011686	None:intergenic
GGAGTTACAGCTGTCAACAG+CGG	0.684832	8.4:-77011707	None:intergenic
GCACGGATAAGACGCCGCAG+TGG	0.685559	8.4:+77012296	MS.gene36957:CDS
AAAACACTCTGCAATGCGTG+TGG	0.694406	8.4:+77012340	MS.gene36957:CDS
TTCGGCGTTTCATCTAACGG+TGG	0.696201	8.4:+77012550	MS.gene36957:CDS
TTTACAGTATGATGATATGG+CGG	0.707206	8.4:+77011919	MS.gene36957:intron
GGTGCCGGAGTATCGACCGG+CGG	0.709828	8.4:+77012387	MS.gene36957:CDS
GAACAATTGTGCCTCCAAAG+CGG	0.725448	8.4:-77011750	None:intergenic
ATCGGTCCTGTTCTCCACTG+CGG	0.751264	8.4:-77012310	None:intergenic
ATGGCGAAAGGCTCACACGG+TGG	0.757233	8.4:-77011586	None:intergenic
GGAAGAAAATGTCTCCACTG+CGG	0.780752	8.4:+77012274	MS.gene36957:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTTCAACTATCATCAATTA+CGG	-	chr8.4:77011861-77011880	None:intergenic	25.0%
!	ATCAATTATCATCATCATTG+TGG	+	chr8.4:77012583-77012602	MS.gene36957:CDS	25.0%
!	GAAATCAAGTAAATCATCAA+TGG	-	chr8.4:77011608-77011627	None:intergenic	25.0%
!	TCAATTATCATCATCATTGT+GGG	+	chr8.4:77012584-77012603	MS.gene36957:CDS	25.0%
!	TTAGTGACATTATAACTTAC+CGG	-	chr8.4:77011823-77011842	None:intergenic	25.0%
!!	TTCACGATTGGATAATTTTT+CGG	-	chr8.4:77012223-77012242	None:intergenic	25.0%
	CTCTTTACAGTATGATGATA+TGG	+	chr8.4:77011916-77011935	MS.gene36957:intron	30.0%
	TAGATAACATGTCTAAACTC+TGG	-	chr8.4:77012610-77012629	None:intergenic	30.0%
	TTTACAGTATGATGATATGG+CGG	+	chr8.4:77011919-77011938	MS.gene36957:intron	30.0%
!	ATTGTTCCTTATGGTTTTTC+CGG	+	chr8.4:77011766-77011785	MS.gene36957:CDS	30.0%
!	GAATTCTCATAGGAAAGTTT+TGG	+	chr8.4:77012441-77012460	MS.gene36957:CDS	30.0%
!	TGTTTTCAATTCTTCTTCAG+CGG	-	chr8.4:77011984-77012003	None:intergenic	30.0%
	AAAACATTGCAACTTCTCTC+CGG	+	chr8.4:77011998-77012017	MS.gene36957:CDS	35.0%
	AGTAAATCATCAATGGCGAA+AGG	-	chr8.4:77011601-77011620	None:intergenic	35.0%
	GAAGCACAAGATTACTTTGT+CGG	+	chr8.4:77011502-77011521	MS.gene36957:CDS	35.0%
!	ATTTTCGGCGTTTCATCTAA+CGG	+	chr8.4:77012547-77012566	MS.gene36957:CDS	35.0%
!	CTGAGTCACAGTTCAATTTT+CGG	+	chr8.4:77012532-77012551	MS.gene36957:CDS	35.0%
!!	ACGATTGGATAATTTTTCGG+TGG	-	chr8.4:77012220-77012239	None:intergenic	35.0%
	ACATCACCGGAAAAACCATA+AGG	-	chr8.4:77011775-77011794	None:intergenic	40.0%
	CTTCCAGAACATTCCACATT+TGG	-	chr8.4:77012259-77012278	None:intergenic	40.0%
	GATCCAAATGTGGAATGTTC+TGG	+	chr8.4:77012253-77012272	MS.gene36957:CDS	40.0%
	TTCTTCGATAACAATGTCGC+CGG	+	chr8.4:77011658-77011677	MS.gene36957:CDS	40.0%
!	CGAAGCATTCGAATTCTCAT+AGG	+	chr8.4:77012431-77012450	MS.gene36957:CDS	40.0%
	AAAACACTCTGCAATGCGTG+TGG	+	chr8.4:77012340-77012359	MS.gene36957:CDS	45.0%
	AAAGCGTGAGGATCCAAATG+TGG	+	chr8.4:77012243-77012262	MS.gene36957:CDS	45.0%
	GAACAATTGTGCCTCCAAAG+CGG	-	chr8.4:77011753-77011772	None:intergenic	45.0%
	GATGAAGATTCCGGTGTTTG+CGG	-	chr8.4:77012040-77012059	None:intergenic	45.0%
	GCACAAGATTACTTTGTCGG+TGG	+	chr8.4:77011505-77011524	MS.gene36957:CDS	45.0%
	GGAAGAAAATGTCTCCACTG+CGG	+	chr8.4:77012274-77012293	MS.gene36957:CDS	45.0%
	GGAATGGCTTTCGAATTTCG+TGG	+	chr8.4:77011946-77011965	MS.gene36957:CDS	45.0%
	TACTTTGTCGGTGGCTACTA+CGG	+	chr8.4:77011514-77011533	MS.gene36957:CDS	45.0%
	TCGGTGGAAGATGAAGATTC+CGG	-	chr8.4:77012049-77012068	None:intergenic	45.0%
	TGCTGTTAAACCGCAAACAC+CGG	+	chr8.4:77012027-77012046	MS.gene36957:CDS	45.0%
	TGCTTCGCCGATACAAAAGT+TGG	-	chr8.4:77012418-77012437	None:intergenic	45.0%
	TGTGGTGTGAGGTTTAAGTC+CGG	+	chr8.4:77012358-77012377	MS.gene36957:CDS	45.0%
	TGTTTGCGGTTTAACAGCAC+CGG	-	chr8.4:77012026-77012045	None:intergenic	45.0%
!	ATGATATGGCGGAACTGGAA+TGG	+	chr8.4:77011930-77011949	MS.gene36957:CDS	45.0%
!	GGAGGCACAATTGTTCCTTA+TGG	+	chr8.4:77011757-77011776	MS.gene36957:CDS	45.0%
!	GGCGAGTCCAACTTTTGTAT+CGG	+	chr8.4:77012408-77012427	MS.gene36957:CDS	45.0%
!	GTATGATGATATGGCGGAAC+TGG	+	chr8.4:77011925-77011944	MS.gene36957:intron	45.0%
!	TCCAATCGTGAAAAAGCGTG+AGG	+	chr8.4:77012231-77012250	MS.gene36957:CDS	45.0%
!!	ACACGCATTGCAGAGTGTTT+TGG	-	chr8.4:77012341-77012360	None:intergenic	45.0%
!!	ATAATTTTTCGGTGGCGCGT+CGG	-	chr8.4:77012212-77012231	None:intergenic	45.0%
!!	CACGCATTGCAGAGTGTTTT+GGG	-	chr8.4:77012340-77012359	None:intergenic	45.0%
!!	TTTCCTGGTAGAGGTGTTTC+TGG	-	chr8.4:77012127-77012146	None:intergenic	45.0%
!!!	TCCTCACGCTTTTTCACGAT+TGG	-	chr8.4:77012235-77012254	None:intergenic	45.0%
	AAAATGTCTCCACTGCGGCA+CGG	+	chr8.4:77012279-77012298	MS.gene36957:CDS	50.0%
	ACCGTCAGACATAATCGCGT+CGG	-	chr8.4:77011638-77011657	None:intergenic	50.0%
	AGCGGTGACATTGGAAGAGT+CGG	-	chr8.4:77011692-77011711	None:intergenic	50.0%
	AGCGTTACGAGCAGTTTCGT+CGG	-	chr8.4:77012092-77012111	None:intergenic	50.0%
	GAGGTTTAAGTCCGGTAGGT+TGG	+	chr8.4:77012366-77012385	MS.gene36957:CDS	50.0%
	GGAAGAGTCGGTGGAATTTC+CGG	-	chr8.4:77011680-77011699	None:intergenic	50.0%
	GGAGTTACAGCTGTCAACAG+CGG	-	chr8.4:77011710-77011729	None:intergenic	50.0%
	GTGTGAGGTTTAAGTCCGGT+AGG	+	chr8.4:77012362-77012381	MS.gene36957:CDS	50.0%
	GTTGACAGCTGTAACTCCTC+TGG	+	chr8.4:77011712-77011731	MS.gene36957:CDS	50.0%
	GTTTCGTCGGTGGAGAATGA+CGG	-	chr8.4:77012079-77012098	None:intergenic	50.0%
	TACCGGAACACAGAGTTCTC+CGG	-	chr8.4:77011806-77011825	None:intergenic	50.0%
	TCAATGGCGAAAGGCTCACA+CGG	-	chr8.4:77011592-77011611	None:intergenic	50.0%
	TCCGGTGATGTTCAACTCAC+CGG	+	chr8.4:77011784-77011803	MS.gene36957:CDS	50.0%
	TTCGGCGTTTCATCTAACGG+TGG	+	chr8.4:77012550-77012569	MS.gene36957:CDS	50.0%
!	AAGGCTCACACGGTGGTTTT+TGG	-	chr8.4:77011582-77011601	None:intergenic	50.0%
!	AGACATAATCGCGTCGGCAT+TGG	-	chr8.4:77011632-77011651	None:intergenic	50.0%
!	CTACGTGCTTTTCCTGGTAG+AGG	-	chr8.4:77012136-77012155	None:intergenic	50.0%
!	GGAGAATGACGGAAGTGTGT+CGG	-	chr8.4:77012068-77012087	None:intergenic	50.0%
!	TCCGGTGAGTTGAACATCAC+CGG	-	chr8.4:77011788-77011807	None:intergenic	50.0%
!	TCTGGTGGTGATAACCGCTT+TGG	+	chr8.4:77011736-77011755	MS.gene36957:CDS	50.0%
!!	CAGAGTGTTTTGGGTCCCAT+CGG	-	chr8.4:77012331-77012350	None:intergenic	50.0%
!!	CGTTTGCTACGTGCTTTTCC+TGG	-	chr8.4:77012142-77012161	None:intergenic	50.0%
!!	CTGGTAGAGGTGTTTCTGGA+CGG	-	chr8.4:77012123-77012142	None:intergenic	50.0%
!!	TAATTTTTCGGTGGCGCGTC+GGG	-	chr8.4:77012211-77012230	None:intergenic	50.0%
	ACAAAAGTTGGACTCGCCGC+CGG	-	chr8.4:77012406-77012425	None:intergenic	55.0%
	ATACTCCGGCACCAACCTAC+CGG	-	chr8.4:77012380-77012399	None:intergenic	55.0%
	ATCGGTCCTGTTCTCCACTG+CGG	-	chr8.4:77012313-77012332	None:intergenic	55.0%
	CACCGGAGAACTCTGTGTTC+CGG	+	chr8.4:77011801-77011820	MS.gene36957:CDS	55.0%
	CAGTGGAGAACAGGACCGAT+GGG	+	chr8.4:77012313-77012332	MS.gene36957:CDS	55.0%
	CGGCGAAAACCAATTCTCGC+CGG	+	chr8.4:77011540-77011559	MS.gene36957:CDS	55.0%
	CGTCCAGAAACACCTCTACC+AGG	+	chr8.4:77012121-77012140	MS.gene36957:CDS	55.0%
	GCCGACGCGATTATGTCTGA+CGG	+	chr8.4:77011634-77011653	MS.gene36957:CDS	55.0%
	GCTGTCAACAGCGGTGACAT+TGG	-	chr8.4:77011701-77011720	None:intergenic	55.0%
	GGTGACATTGGAAGAGTCGG+TGG	-	chr8.4:77011689-77011708	None:intergenic	55.0%
	GGTGGTGATAACCGCTTTGG+AGG	+	chr8.4:77011739-77011758	MS.gene36957:CDS	55.0%
	GTTACGAGCAGTTTCGTCGG+TGG	-	chr8.4:77012089-77012108	None:intergenic	55.0%
	GTTATCACCACCAGAGCCAG+AGG	-	chr8.4:77011731-77011750	None:intergenic	55.0%
	TCTGCAATGCGTGTGGTGTG+AGG	+	chr8.4:77012347-77012366	MS.gene36957:CDS	55.0%
!	AGCTGTAACTCCTCTGGCTC+TGG	+	chr8.4:77011718-77011737	MS.gene36957:CDS	55.0%
!	GAATGACGGAAGTGTGTCGG+TGG	-	chr8.4:77012065-77012084	None:intergenic	55.0%
!!	AATTTTTCGGTGGCGCGTCG+GGG	-	chr8.4:77012210-77012229	None:intergenic	55.0%
!!	TAAGTCCGGTAGGTTGGTGC+CGG	+	chr8.4:77012372-77012391	MS.gene36957:CDS	55.0%
!!	TGTAACTCCTCTGGCTCTGG+TGG	+	chr8.4:77011721-77011740	MS.gene36957:CDS	55.0%
!!!	CGGCGAGAATTGGTTTTCGC+CGG	-	chr8.4:77011542-77011561	None:intergenic	55.0%
	AAACGATCACGCGCTGCTCC+AGG	+	chr8.4:77012157-77012176	MS.gene36957:CDS	60.0%
	AAGACGCCGCAGTGGAGAAC+AGG	+	chr8.4:77012304-77012323	MS.gene36957:CDS	60.0%
	ATGGCGAAAGGCTCACACGG+TGG	-	chr8.4:77011589-77011608	None:intergenic	60.0%
	GACGCTTCTCCGGCGAGAAT+TGG	-	chr8.4:77011552-77011571	None:intergenic	60.0%
	GCAGTGGAGAACAGGACCGA+TGG	+	chr8.4:77012312-77012331	MS.gene36957:CDS	60.0%
	TGGTCACCGTGACGCTTCTC+CGG	-	chr8.4:77011562-77011581	None:intergenic	60.0%
	TTGCAACTTCTCTCCGGCGC+CGG	+	chr8.4:77012004-77012023	MS.gene36957:CDS	60.0%
!!	GTTGGTGCCGGAGTATCGAC+CGG	+	chr8.4:77012384-77012403	MS.gene36957:CDS	60.0%
!!	TTCTCGCCGGAGAAGCGTCA+CGG	+	chr8.4:77011553-77011572	MS.gene36957:CDS	60.0%
	GCACGGATAAGACGCCGCAG+TGG	+	chr8.4:77012296-77012315	MS.gene36957:CDS	65.0%
	GCGCGTCGGGGAGATGAAGT+AGG	-	chr8.4:77012198-77012217	None:intergenic	65.0%
	GCGTCTTATCCGTGCCGCAG+TGG	-	chr8.4:77012291-77012310	None:intergenic	65.0%
	GGGGAGATGAAGTAGGCGCG+TGG	-	chr8.4:77012191-77012210	None:intergenic	65.0%
	GTCGGTGGCTACTACGGTGC+CGG	+	chr8.4:77011520-77011539	MS.gene36957:CDS	65.0%
!!	CGGTTTAACAGCACCGGCGC+CGG	-	chr8.4:77012020-77012039	None:intergenic	65.0%
	AGGCGCGTGGACCAGTCTCC+TGG	-	chr8.4:77012178-77012197	None:intergenic	70.0%
	CACGCGCTGCTCCAGGAGAC+TGG	+	chr8.4:77012164-77012183	MS.gene36957:CDS	70.0%
	CTCGCCGCCGGTCGATACTC+CGG	-	chr8.4:77012394-77012413	None:intergenic	70.0%
!!	GGTGCCGGAGTATCGACCGG+CGG	+	chr8.4:77012387-77012406	MS.gene36957:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	77011499	77012630	77011499	ID=MS.gene36957
chr8.4	mRNA	77011499	77012630	77011499	ID=MS.gene36957.t1;Parent=MS.gene36957
chr8.4	exon	77011499	77011822	77011499	ID=MS.gene36957.t1.exon1;Parent=MS.gene36957.t1
chr8.4	CDS	77011499	77011822	77011499	ID=cds.MS.gene36957.t1;Parent=MS.gene36957.t1
chr8.4	exon	77011926	77012630	77011926	ID=MS.gene36957.t1.exon2;Parent=MS.gene36957.t1
chr8.4	CDS	77011926	77012630	77011926	ID=cds.MS.gene36957.t1;Parent=MS.gene36957.t1

Gene Sequence

>MS.gene36957

ATGGAAGCACAAGATTACTTTGTCGGTGGCTACTACGGTGCCGGCGAAAACCAATTCTCGCCGGAGAAGCGTCACGGTGACCAAAAACCACCGTGTGAGCCTTTCGCCATTGATGATTTACTTGATTTCTCCAATGCCGACGCGATTATGTCTGACGGTTTCTTCGATAACAATGTCGCCGGAAATTCCACCGACTCTTCCAATGTCACCGCTGTTGACAGCTGTAACTCCTCTGGCTCTGGTGGTGATAACCGCTTTGGAGGCACAATTGTTCCTTATGGTTTTTCCGGTGATGTTCAACTCACCGGAGAACTCTGTGTTCCGGTAAGTTATAATGTCACTAATTCAGCTTAATTTAACCGTAATTGATGATAGTTGAAGTTCAATTTCGAATTATGAAACTGAATTTGAATTTTACTCTTTACAGTATGATGATATGGCGGAACTGGAATGGCTTTCGAATTTCGTGGAAGATTCATACTCCGCTGAAGAAGAATTGAAAACATTGCAACTTCTCTCCGGCGCCGGTGCTGTTAAACCGCAAACACCGGAATCTTCATCTTCCACCGACACACTTCCGTCATTCTCCACCGACGAAACTGCTCGTAACGCTTCATTTCTCCGTCCAGAAACACCTCTACCAGGAAAAGCACGTAGCAAACGATCACGCGCTGCTCCAGGAGACTGGTCCACGCGCCTACTTCATCTCCCCGACGCGCCACCGAAAAATTATCCAATCGTGAAAAAGCGTGAGGATCCAAATGTGGAATGTTCTGGAAGAAAATGTCTCCACTGCGGCACGGATAAGACGCCGCAGTGGAGAACAGGACCGATGGGACCCAAAACACTCTGCAATGCGTGTGGTGTGAGGTTTAAGTCCGGTAGGTTGGTGCCGGAGTATCGACCGGCGGCGAGTCCAACTTTTGTATCGGCGAAGCATTCGAATTCTCATAGGAAAGTTTTGGAACTTCGAAGACAGAAAGAGATGCAAAGATCATCACAGCATCATCATCATCATCAACATCAACAGCTTCTGAGTCACAGTTCAATTTTCGGCGTTTCATCTAACGGTGGAGATGATTTCATCAATTATCATCATCATTGTGGGCCAGAGTTTAGACATGTTATCTAG

Protein sequence

>MS.gene36957.t1

MEAQDYFVGGYYGAGENQFSPEKRHGDQKPPCEPFAIDDLLDFSNADAIMSDGFFDNNVAGNSTDSSNVTAVDSCNSSGSGGDNRFGGTIVPYGFSGDVQLTGELCVPYDDMAELEWLSNFVEDSYSAEEELKTLQLLSGAGAVKPQTPESSSSTDTLPSFSTDETARNASFLRPETPLPGKARSKRSRAAPGDWSTRLLHLPDAPPKNYPIVKKREDPNVECSGRKCLHCGTDKTPQWRTGPMGPKTLCNACGVRFKSGRLVPEYRPAASPTFVSAKHSNSHRKVLELRRQKEMQRSSQHHHHHQHQQLLSHSSIFGVSSNGGDDFINYHHHCGPEFRHVI