Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38683.t1 | RHN59613.1 | 100 | 99 | 0 | 0 | 1 | 99 | 48 | 146 | 1.50E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38683.t1 | Q1RMP0 | 38.0 | 92 | 57 | 0 | 8 | 99 | 31 | 122 | 1.3e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38683.t1 | G7JQD3 | 100.0 | 99 | 0 | 0 | 1 | 99 | 17 | 115 | 1.1e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene38683 | MS.gene064140 | PPI |
| MS.gene38683 | MS.gene030166 | PPI |
| MS.gene022098 | MS.gene38683 | PPI |
| MS.gene38683 | MS.gene047813 | PPI |
| MS.gene38683 | MS.gene39636 | PPI |
| MS.gene43180 | MS.gene38683 | PPI |
| MS.gene38683 | MS.gene053492 | PPI |
| MS.gene38683 | MS.gene26516 | PPI |
| MS.gene38683 | MS.gene97308 | PPI |
| MS.gene26777 | MS.gene38683 | PPI |
| MS.gene38683 | MS.gene68633 | PPI |
| MS.gene38683 | MS.gene95440 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38683.t1 | MTR_3g102420 | 100.000 | 99 | 0 | 0 | 1 | 99 | 17 | 115 | 1.51e-70 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38683.t1 | AT3G25940 | 60.396 | 101 | 37 | 1 | 1 | 98 | 18 | 118 | 6.69e-37 | 121 |
| MS.gene38683.t1 | AT1G20065 | 59.091 | 44 | 18 | 0 | 1 | 44 | 18 | 61 | 2.25e-11 | 54.7 |
| MS.gene38683.t1 | AT1G20065 | 59.091 | 44 | 18 | 0 | 1 | 44 | 18 | 61 | 2.25e-11 | 54.7 |
| MS.gene38683.t1 | AT1G20065 | 59.091 | 44 | 18 | 0 | 1 | 44 | 18 | 61 | 2.25e-11 | 54.7 |
| MS.gene38683.t1 | AT1G20065 | 59.091 | 44 | 18 | 0 | 1 | 44 | 18 | 61 | 2.25e-11 | 54.7 |
Find 19 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATACCTGTCTGGTATAAA+AGG | 0.277991 | 3.3:-84664714 | None:intergenic |
| CAAAACCAAGTGTAACATAA+AGG | 0.353007 | 3.3:+84662995 | MS.gene38683:CDS |
| ACAGAAGGTGCAATTGTCTA+AGG | 0.372837 | 3.3:+84664571 | MS.gene38683:CDS |
| CAGATGAGATCGGCGGATGA+AGG | 0.477672 | 3.3:+84664820 | MS.gene38683:intron |
| AAAACCAAGTGTAACATAAA+GGG | 0.477760 | 3.3:+84662996 | MS.gene38683:CDS |
| GAAGTCTAGTAATACCTGTC+TGG | 0.505310 | 3.3:-84664723 | None:intergenic |
| TAGGATATCAGAAGAGAACT+TGG | 0.517197 | 3.3:+84664530 | MS.gene38683:intron |
| AGATGAGATCGGCGGATGAA+GGG | 0.551664 | 3.3:+84664821 | MS.gene38683:intron |
| TTGAAACTGATGACCACAGC+GGG | 0.553103 | 3.3:-84664866 | None:intergenic |
| AGTACCCTTTATGTTACACT+TGG | 0.559345 | 3.3:-84663000 | None:intergenic |
| TGATTTAATCGAGGAACAGA+AGG | 0.563158 | 3.3:+84664556 | MS.gene38683:CDS |
| CATAGTGCATAATCAGTTGA+GGG | 0.577852 | 3.3:-84662963 | None:intergenic |
| TTCCTAATTTCAGATGAGAT+CGG | 0.584414 | 3.3:+84664810 | MS.gene38683:intron |
| GCATAGTGCATAATCAGTTG+AGG | 0.598932 | 3.3:-84662964 | None:intergenic |
| AAGTTACACAATTTCTGCTG+AGG | 0.612146 | 3.3:+84663861 | MS.gene38683:CDS |
| ACTTGGAATTGATTTAATCG+AGG | 0.619946 | 3.3:+84664547 | MS.gene38683:CDS |
| CTAATTTCAGATGAGATCGG+CGG | 0.632502 | 3.3:+84664813 | MS.gene38683:intron |
| TTCTACACATGCACCCGCTG+TGG | 0.636738 | 3.3:+84664853 | MS.gene38683:CDS |
| CTTGAAACTGATGACCACAG+CGG | 0.759797 | 3.3:-84664867 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTACAATATATATTTAA+TGG | + | chr3.3:84664600-84664619 | MS.gene38683:intron | 10.0% |
| !!! | CTAATATATTTATTGATTAT+TGG | + | chr3.3:84664478-84664497 | MS.gene38683:intron | 10.0% |
| !! | AAATAAGCTTCAAATTAAAT+TGG | - | chr3.3:84664763-84664782 | None:intergenic | 15.0% |
| !! | AAATTATATCTAAATATGCA+AGG | - | chr3.3:84663725-84663744 | None:intergenic | 15.0% |
| !! | ATATAATCATATCATATGTT+AGG | - | chr3.3:84663937-84663956 | None:intergenic | 15.0% |
| !! | TTTAATATCTGAAAATTACA+AGG | - | chr3.3:84663830-84663849 | None:intergenic | 15.0% |
| !!! | TTGTAATTTTCAGATATTAA+AGG | + | chr3.3:84663829-84663848 | MS.gene38683:intron | 15.0% |
| !! | AAAAAAATTAGGTGACTTTA+TGG | + | chr3.3:84663473-84663492 | MS.gene38683:intron | 20.0% |
| !! | AAAATTAGAAGAAAGAAGTT+TGG | - | chr3.3:84664093-84664112 | None:intergenic | 20.0% |
| !! | AACTAATATATTGTGTGTTT+AGG | + | chr3.3:84663776-84663795 | MS.gene38683:intron | 20.0% |
| !! | ACATATATGGAAATAACATT+AGG | + | chr3.3:84663314-84663333 | MS.gene38683:intron | 20.0% |
| !! | ACATTTATTACAACTTAACA+GGG | - | chr3.3:84663553-84663572 | None:intergenic | 20.0% |
| !! | CTTAAATGGTTTACTAATTT+GGG | + | chr3.3:84663117-84663136 | MS.gene38683:intron | 20.0% |
| !! | TATTAAGATCATTGCTTAAA+TGG | + | chr3.3:84663103-84663122 | MS.gene38683:intron | 20.0% |
| !!! | AAACTTCTTTCTTCTAATTT+TGG | + | chr3.3:84664092-84664111 | MS.gene38683:intron | 20.0% |
| !!! | ATTTTCAGATATTAAAGGAA+AGG | + | chr3.3:84663834-84663853 | MS.gene38683:intron | 20.0% |
| ! | AAAACCAAGTGTAACATAAA+GGG | + | chr3.3:84662996-84663015 | MS.gene38683:CDS | 25.0% |
| ! | AACAAAACATGTGAAAAATG+TGG | + | chr3.3:84664677-84664696 | MS.gene38683:CDS | 25.0% |
| ! | ATAGATGATATATGATACGA+GGG | - | chr3.3:84664170-84664189 | None:intergenic | 25.0% |
| ! | CATTTATTACAACTTAACAG+GGG | - | chr3.3:84663552-84663571 | None:intergenic | 25.0% |
| ! | CTAATGTTATGTTTCTTTAC+AGG | + | chr3.3:84664652-84664671 | MS.gene38683:intron | 25.0% |
| ! | CTTAGAGTGAAAAGAAAATT+TGG | + | chr3.3:84663175-84663194 | MS.gene38683:intron | 25.0% |
| ! | GACATTTATTACAACTTAAC+AGG | - | chr3.3:84663554-84663573 | None:intergenic | 25.0% |
| ! | GCTTAAATGGTTTACTAATT+TGG | + | chr3.3:84663116-84663135 | MS.gene38683:intron | 25.0% |
| ! | TCAATTTCATCTAAATACAC+TGG | - | chr3.3:84664201-84664220 | None:intergenic | 25.0% |
| ! | TGATATCAAATCTTAAACCT+AGG | - | chr3.3:84664410-84664429 | None:intergenic | 25.0% |
| AATTACAAGGACATTCAGTT+AGG | - | chr3.3:84663817-84663836 | None:intergenic | 30.0% | |
| AATTATCCTCATCTACTTTG+TGG | + | chr3.3:84664503-84664522 | MS.gene38683:intron | 30.0% | |
| ACAGAGCAGATTTAATATAC+AGG | + | chr3.3:84663365-84663384 | MS.gene38683:intron | 30.0% | |
| CAAAACCAAGTGTAACATAA+AGG | + | chr3.3:84662995-84663014 | MS.gene38683:CDS | 30.0% | |
| CAGAGCAGATTTAATATACA+GGG | + | chr3.3:84663366-84663385 | MS.gene38683:intron | 30.0% | |
| CATAGATGATATATGATACG+AGG | - | chr3.3:84664171-84664190 | None:intergenic | 30.0% | |
| CTATGATAAACTGTGTTTGT+TGG | + | chr3.3:84664266-84664285 | MS.gene38683:intron | 30.0% | |
| GGAATCTTCATGATTCTTTA+AGG | + | chr3.3:84663501-84663520 | MS.gene38683:intron | 30.0% | |
| GTAGGTGATCAAATAACTAA+TGG | + | chr3.3:84663201-84663220 | MS.gene38683:intron | 30.0% | |
| GTTTAGGCTGCATAATATAT+AGG | + | chr3.3:84663274-84663293 | MS.gene38683:intron | 30.0% | |
| TAATACCTGTCTGGTATAAA+AGG | - | chr3.3:84664717-84664736 | None:intergenic | 30.0% | |
| TAGATGATATATGATACGAG+GGG | - | chr3.3:84664169-84664188 | None:intergenic | 30.0% | |
| TCATATCATATGTTAGGAGA+TGG | - | chr3.3:84663931-84663950 | None:intergenic | 30.0% | |
| TCTCATCTTGTTAATGCTTA+TGG | + | chr3.3:84663572-84663591 | MS.gene38683:intron | 30.0% | |
| TTCCTAATTTCAGATGAGAT+CGG | + | chr3.3:84664810-84664829 | MS.gene38683:intron | 30.0% | |
| ! | ACTTGGAATTGATTTAATCG+AGG | + | chr3.3:84664547-84664566 | MS.gene38683:CDS | 30.0% |
| ! | TATTGTGACTTTGTTCTAGA+AGG | - | chr3.3:84664146-84664165 | None:intergenic | 30.0% |
| !! | ATTCTGATTTTGAAAGAGCA+AGG | + | chr3.3:84663241-84663260 | MS.gene38683:intron | 30.0% |
| !! | ATTGTGACTTTGTTCTAGAA+GGG | - | chr3.3:84664145-84664164 | None:intergenic | 30.0% |
| !! | GAAAAGAAAATTTGGTGTGT+AGG | + | chr3.3:84663183-84663202 | MS.gene38683:intron | 30.0% |
| !!! | GCTGATAGATTATTTTGAAG+AGG | - | chr3.3:84663040-84663059 | None:intergenic | 30.0% |
| !!! | GCTTTTTGAAATATCAACCT+AGG | + | chr3.3:84664390-84664409 | MS.gene38683:intron | 30.0% |
| AAGTTACACAATTTCTGCTG+AGG | + | chr3.3:84663861-84663880 | MS.gene38683:CDS | 35.0% | |
| ACATGTGAAAAATGTGGTCA+TGG | + | chr3.3:84664683-84664702 | MS.gene38683:CDS | 35.0% | |
| AGATTTAATATACAGGGACG+CGG | + | chr3.3:84663372-84663391 | MS.gene38683:intron | 35.0% | |
| AGTACCCTTTATGTTACACT+TGG | - | chr3.3:84663003-84663022 | None:intergenic | 35.0% | |
| ATTCAGTTAGGACGCATAAT+GGG | - | chr3.3:84663805-84663824 | None:intergenic | 35.0% | |
| CATAGTGCATAATCAGTTGA+GGG | - | chr3.3:84662966-84662985 | None:intergenic | 35.0% | |
| GCAAGGTGTAAAGATTGTTT+AGG | + | chr3.3:84663258-84663277 | MS.gene38683:intron | 35.0% | |
| TAGGATATCAGAAGAGAACT+TGG | + | chr3.3:84664530-84664549 | MS.gene38683:intron | 35.0% | |
| TAGTCTCCAGAAAATAGAGA+AGG | - | chr3.3:84664121-84664140 | None:intergenic | 35.0% | |
| TATATGTGCCTATGTCATAG+TGG | - | chr3.3:84664236-84664255 | None:intergenic | 35.0% | |
| TGATTTAATCGAGGAACAGA+AGG | + | chr3.3:84664556-84664575 | MS.gene38683:CDS | 35.0% | |
| TTTCCATATATGTGTGTAGC+TGG | - | chr3.3:84663307-84663326 | None:intergenic | 35.0% | |
| ! | ATCACTATGTTATGCTGCTT+AGG | + | chr3.3:84663955-84663974 | MS.gene38683:intron | 35.0% |
| ! | GATCTCATCTGAAATTAGGA+AGG | - | chr3.3:84664811-84664830 | None:intergenic | 35.0% |
| ! | TTAGGTGACTTTATGGATTG+AGG | + | chr3.3:84663480-84663499 | MS.gene38683:intron | 35.0% |
| ! | TTATGCTGCTTAGGCTTATA+GGG | + | chr3.3:84663964-84663983 | MS.gene38683:intron | 35.0% |
| !! | ACCTTGATTTGCTTTGTCTT+GGG | + | chr3.3:84664307-84664326 | MS.gene38683:intron | 35.0% |
| !! | TACCTTGATTTGCTTTGTCT+TGG | + | chr3.3:84664306-84664325 | MS.gene38683:intron | 35.0% |
| !!! | TTACACTTGGTTTTGCACAA+GGG | - | chr3.3:84662990-84663009 | None:intergenic | 35.0% |
| ACATTAGGTGACATCTCTGA+TGG | + | chr3.3:84663329-84663348 | MS.gene38683:intron | 40.0% | |
| ATCCTCATCTACTTTGTGGT+TGG | + | chr3.3:84664507-84664526 | MS.gene38683:intron | 40.0% | |
| ATGCCAGCTACACACATATA+TGG | + | chr3.3:84663301-84663320 | MS.gene38683:intron | 40.0% | |
| CATTCAGTTAGGACGCATAA+TGG | - | chr3.3:84663806-84663825 | None:intergenic | 40.0% | |
| CTAATTTCAGATGAGATCGG+CGG | + | chr3.3:84664813-84664832 | MS.gene38683:intron | 40.0% | |
| GAAGTCTAGTAATACCTGTC+TGG | - | chr3.3:84664726-84664745 | None:intergenic | 40.0% | |
| GCATAGTGCATAATCAGTTG+AGG | - | chr3.3:84662967-84662986 | None:intergenic | 40.0% | |
| GGTCTACTGATATGTGCATT+GGG | + | chr3.3:84663985-84664004 | MS.gene38683:intron | 40.0% | |
| GTGACATCTCTGATGGTAAA+AGG | + | chr3.3:84663336-84663355 | MS.gene38683:intron | 40.0% | |
| TACCAACCACAAAGTAGATG+AGG | - | chr3.3:84664512-84664531 | None:intergenic | 40.0% | |
| TCATCTACTTTGTGGTTGGT+AGG | + | chr3.3:84664511-84664530 | MS.gene38683:intron | 40.0% | |
| TCCCAAGACAAAGCAAATCA+AGG | - | chr3.3:84664311-84664330 | None:intergenic | 40.0% | |
| TCTACTGCTTGATACTCCTT+AGG | + | chr3.3:84663666-84663685 | MS.gene38683:intron | 40.0% | |
| TTGATACTCCTTAGGTGTAG+TGG | + | chr3.3:84663674-84663693 | MS.gene38683:intron | 40.0% | |
| ! | ACAGAAGGTGCAATTGTCTA+AGG | + | chr3.3:84664571-84664590 | MS.gene38683:CDS | 40.0% |
| ! | ATGGTTTCGCAAAGTATCTG+TGG | + | chr3.3:84663591-84663610 | MS.gene38683:intron | 40.0% |
| ! | GTTATGCTGCTTAGGCTTAT+AGG | + | chr3.3:84663963-84663982 | MS.gene38683:intron | 40.0% |
| ! | TGGCTTCCTTCTCTATTTTC+TGG | + | chr3.3:84664112-84664131 | MS.gene38683:intron | 40.0% |
| ! | TGGTTTCGCAAAGTATCTGT+GGG | + | chr3.3:84663592-84663611 | MS.gene38683:intron | 40.0% |
| !!! | GTTACACTTGGTTTTGCACA+AGG | - | chr3.3:84662991-84663010 | None:intergenic | 40.0% |
| !!! | TACACTTGGTTTTGCACAAG+GGG | - | chr3.3:84662989-84663008 | None:intergenic | 40.0% |
| ATCCACCTCCACTATGACAT+AGG | + | chr3.3:84664225-84664244 | MS.gene38683:intron | 45.0% | |
| ATGTGCCTATGTCATAGTGG+AGG | - | chr3.3:84664233-84664252 | None:intergenic | 45.0% | |
| CTTGAAACTGATGACCACAG+CGG | - | chr3.3:84664870-84664889 | None:intergenic | 45.0% | |
| GGGTCTACTGATATGTGCAT+TGG | + | chr3.3:84663984-84664003 | MS.gene38683:intron | 45.0% | |
| TTGAAACTGATGACCACAGC+GGG | - | chr3.3:84664869-84664888 | None:intergenic | 45.0% | |
| TTGCTTTGTCTTGGGACTCT+TGG | + | chr3.3:84664315-84664334 | MS.gene38683:intron | 45.0% | |
| TTTGTCTTGGGACTCTTGGT+GGG | + | chr3.3:84664319-84664338 | MS.gene38683:intron | 45.0% | |
| ! | AGCTGCCTTTTATACCAGAC+AGG | + | chr3.3:84664709-84664728 | MS.gene38683:CDS | 45.0% |
| ! | CGCCGATCTCATCTGAAATT+AGG | - | chr3.3:84664815-84664834 | None:intergenic | 45.0% |
| ! | TGTGTCTGAGTTTTGCTGCT+AGG | + | chr3.3:84663431-84663450 | MS.gene38683:intron | 45.0% |
| !! | ACACACTAAGTGCTGAGAAG+TGG | - | chr3.3:84663416-84663435 | None:intergenic | 45.0% |
| !! | ACACTAAGTGCTGAGAAGTG+GGG | - | chr3.3:84663414-84663433 | None:intergenic | 45.0% |
| !! | CACACTAAGTGCTGAGAAGT+GGG | - | chr3.3:84663415-84663434 | None:intergenic | 45.0% |
| !! | AACAAAAAAAAAAAAAAATT+AGG | + | chr3.3:84663462-84663481 | MS.gene38683:intron | 5.0% |
| AGATGAGATCGGCGGATGAA+GGG | + | chr3.3:84664821-84664840 | MS.gene38683:intron | 50.0% | |
| CTTTGTCTTGGGACTCTTGG+TGG | + | chr3.3:84664318-84664337 | MS.gene38683:intron | 50.0% | |
| GACGTGTACCACTACACCTA+AGG | - | chr3.3:84663685-84663704 | None:intergenic | 50.0% | |
| TGCTGAGAAGTGGGGAATTG+CGG | - | chr3.3:84663406-84663425 | None:intergenic | 50.0% | |
| ! | TGCCTATGTCATAGTGGAGG+TGG | - | chr3.3:84664230-84664249 | None:intergenic | 50.0% |
| CAGATGAGATCGGCGGATGA+AGG | + | chr3.3:84664820-84664839 | MS.gene38683:intron | 55.0% | |
| GCTGAGAAGTGGGGAATTGC+GGG | - | chr3.3:84663405-84663424 | None:intergenic | 55.0% | |
| TTCTACACATGCACCCGCTG+TGG | + | chr3.3:84664853-84664872 | MS.gene38683:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 84662951 | 84664897 | 84662951 | ID=MS.gene38683 |
| chr3.3 | mRNA | 84662951 | 84664897 | 84662951 | ID=MS.gene38683.t1;Parent=MS.gene38683 |
| chr3.3 | exon | 84662951 | 84663017 | 84662951 | ID=MS.gene38683.t1.exon1;Parent=MS.gene38683.t1 |
| chr3.3 | CDS | 84662951 | 84663017 | 84662951 | ID=cds.MS.gene38683.t1;Parent=MS.gene38683.t1 |
| chr3.3 | exon | 84663842 | 84663882 | 84663842 | ID=MS.gene38683.t1.exon2;Parent=MS.gene38683.t1 |
| chr3.3 | CDS | 84663842 | 84663882 | 84663842 | ID=cds.MS.gene38683.t1;Parent=MS.gene38683.t1 |
| chr3.3 | exon | 84664533 | 84664592 | 84664533 | ID=MS.gene38683.t1.exon3;Parent=MS.gene38683.t1 |
| chr3.3 | CDS | 84664533 | 84664592 | 84664533 | ID=cds.MS.gene38683.t1;Parent=MS.gene38683.t1 |
| chr3.3 | exon | 84664674 | 84664730 | 84664674 | ID=MS.gene38683.t1.exon4;Parent=MS.gene38683.t1 |
| chr3.3 | CDS | 84664674 | 84664730 | 84664674 | ID=cds.MS.gene38683.t1;Parent=MS.gene38683.t1 |
| chr3.3 | exon | 84664823 | 84664897 | 84664823 | ID=MS.gene38683.t1.exon5;Parent=MS.gene38683.t1 |
| chr3.3 | CDS | 84664823 | 84664897 | 84664823 | ID=cds.MS.gene38683.t1;Parent=MS.gene38683.t1 |
| Gene Sequence |
| Protein sequence |
