Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39264.t1 | RHN79377.1 | 91.8 | 98 | 8 | 0 | 1 | 98 | 322 | 419 | 1.10E-45 | 192.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39264.t1 | A0A072VJR7 | 91.8 | 98 | 8 | 0 | 1 | 98 | 221 | 318 | 8.2e-46 | 192.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050752 | MS.gene39264 | 0.802422 | 5.65E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene39264 | 0.820929 | 5.29E-53 | -1.69E-46 |
| MS.gene051902 | MS.gene39264 | 0.821372 | 4.18E-53 | -1.69E-46 |
| MS.gene052947 | MS.gene39264 | 0.8058 | 1.12E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene39264 | 0.801363 | 9.33E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene39264 | 0.801157 | 1.03E-48 | -1.69E-46 |
| MS.gene057102 | MS.gene39264 | 0.816287 | 5.98E-52 | -1.69E-46 |
| MS.gene057705 | MS.gene39264 | 0.814142 | 1.79E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene39264 | 0.80322 | 3.87E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene39264 | 0.800669 | 1.29E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39264.t1 | MTR_1g055175 | 91.837 | 98 | 8 | 0 | 1 | 98 | 104 | 201 | 1.02e-62 | 189 |
| MS.gene39264.t1 | MTR_1g055165 | 91.837 | 98 | 8 | 0 | 1 | 98 | 221 | 318 | 3.88e-62 | 191 |
| MS.gene39264.t1 | MTR_1g055135 | 75.510 | 98 | 23 | 1 | 1 | 98 | 279 | 375 | 3.27e-43 | 144 |
| MS.gene39264.t1 | MTR_1g055195 | 70.213 | 94 | 26 | 2 | 5 | 98 | 387 | 478 | 1.92e-39 | 136 |
| MS.gene39264.t1 | MTR_5g097210 | 64.894 | 94 | 32 | 1 | 1 | 94 | 267 | 359 | 7.20e-37 | 127 |
| MS.gene39264.t1 | MTR_4g109280 | 64.286 | 98 | 30 | 4 | 4 | 98 | 264 | 359 | 4.14e-33 | 117 |
| MS.gene39264.t1 | MTR_4g118370 | 58.696 | 92 | 36 | 2 | 4 | 95 | 168 | 257 | 7.32e-32 | 112 |
| MS.gene39264.t1 | MTR_4g117720 | 57.692 | 104 | 38 | 4 | 1 | 98 | 275 | 378 | 3.15e-30 | 110 |
| MS.gene39264.t1 | MTR_1g055250 | 75.362 | 69 | 16 | 1 | 5 | 73 | 177 | 244 | 5.55e-30 | 108 |
| MS.gene39264.t1 | MTR_6g060650 | 57.732 | 97 | 39 | 2 | 2 | 97 | 277 | 372 | 9.19e-30 | 108 |
| MS.gene39264.t1 | MTR_5g023000 | 54.000 | 100 | 36 | 3 | 4 | 98 | 5 | 99 | 1.18e-28 | 99.8 |
| MS.gene39264.t1 | MTR_4g118400 | 59.140 | 93 | 34 | 3 | 3 | 93 | 205 | 295 | 1.36e-28 | 104 |
| MS.gene39264.t1 | MTR_4g118360 | 51.020 | 98 | 46 | 2 | 2 | 98 | 30 | 126 | 3.62e-27 | 96.7 |
| MS.gene39264.t1 | MTR_3g103020 | 56.000 | 100 | 34 | 3 | 4 | 98 | 284 | 378 | 4.72e-27 | 102 |
| MS.gene39264.t1 | MTR_4g109270 | 55.446 | 101 | 35 | 3 | 3 | 98 | 269 | 364 | 1.11e-26 | 100 |
| MS.gene39264.t1 | MTR_4g117750 | 54.945 | 91 | 39 | 2 | 4 | 94 | 267 | 355 | 5.75e-26 | 100 |
| MS.gene39264.t1 | MTR_4g117750 | 47.872 | 94 | 47 | 2 | 4 | 96 | 389 | 481 | 1.24e-21 | 87.8 |
| MS.gene39264.t1 | MTR_4g117530 | 51.042 | 96 | 42 | 2 | 4 | 98 | 162 | 253 | 6.60e-26 | 96.7 |
| MS.gene39264.t1 | MTR_8g433040 | 50.526 | 95 | 44 | 1 | 3 | 97 | 272 | 363 | 2.09e-25 | 97.4 |
| MS.gene39264.t1 | MTR_8g032380 | 50.526 | 95 | 44 | 1 | 3 | 97 | 272 | 363 | 2.09e-25 | 97.4 |
| MS.gene39264.t1 | MTR_4g116320 | 52.128 | 94 | 43 | 2 | 3 | 95 | 163 | 255 | 1.03e-24 | 93.6 |
| MS.gene39264.t1 | MTR_4g117850 | 53.763 | 93 | 34 | 2 | 4 | 91 | 285 | 373 | 1.10e-24 | 95.5 |
| MS.gene39264.t1 | MTR_8g040935 | 54.545 | 99 | 39 | 4 | 4 | 98 | 112 | 208 | 5.10e-24 | 90.9 |
| MS.gene39264.t1 | MTR_4g117770 | 50.000 | 96 | 46 | 2 | 3 | 97 | 272 | 366 | 6.52e-24 | 93.2 |
| MS.gene39264.t1 | MTR_4g117810 | 50.000 | 96 | 46 | 2 | 3 | 97 | 272 | 366 | 6.52e-24 | 93.2 |
| MS.gene39264.t1 | MTR_4g117780 | 50.000 | 96 | 46 | 2 | 3 | 97 | 272 | 366 | 7.85e-24 | 93.2 |
| MS.gene39264.t1 | MTR_4g023380 | 48.485 | 99 | 43 | 4 | 3 | 97 | 284 | 378 | 8.04e-23 | 90.5 |
| MS.gene39264.t1 | MTR_3g034180 | 53.763 | 93 | 40 | 3 | 6 | 97 | 398 | 488 | 2.10e-22 | 90.1 |
| MS.gene39264.t1 | MTR_4g023440 | 48.980 | 98 | 42 | 4 | 3 | 97 | 239 | 331 | 2.68e-22 | 88.6 |
| MS.gene39264.t1 | MTR_4g117870 | 46.316 | 95 | 50 | 1 | 4 | 98 | 174 | 267 | 2.85e-22 | 87.4 |
| MS.gene39264.t1 | MTR_4g116330 | 47.959 | 98 | 47 | 3 | 4 | 98 | 245 | 341 | 4.50e-22 | 88.2 |
| MS.gene39264.t1 | MTR_4g116330 | 47.959 | 98 | 47 | 3 | 4 | 98 | 284 | 380 | 4.53e-22 | 88.6 |
| MS.gene39264.t1 | MTR_5g070080 | 53.061 | 98 | 42 | 4 | 3 | 98 | 291 | 386 | 8.86e-22 | 87.8 |
| MS.gene39264.t1 | MTR_4g023080 | 47.917 | 96 | 44 | 3 | 3 | 96 | 261 | 352 | 2.10e-21 | 86.3 |
| MS.gene39264.t1 | MTR_4g023130 | 48.958 | 96 | 43 | 3 | 3 | 96 | 220 | 311 | 2.34e-21 | 85.9 |
| MS.gene39264.t1 | MTR_6g463430 | 48.421 | 95 | 44 | 2 | 4 | 98 | 113 | 202 | 5.32e-21 | 82.8 |
| MS.gene39264.t1 | MTR_4g118380 | 57.971 | 69 | 26 | 2 | 3 | 69 | 289 | 356 | 9.06e-21 | 84.7 |
| MS.gene39264.t1 | MTR_4g094365 | 44.681 | 94 | 51 | 1 | 4 | 97 | 271 | 363 | 2.24e-20 | 83.6 |
| MS.gene39264.t1 | MTR_5g070130 | 51.087 | 92 | 41 | 3 | 3 | 92 | 281 | 370 | 3.26e-20 | 83.6 |
| MS.gene39264.t1 | MTR_4g122850 | 43.878 | 98 | 51 | 3 | 3 | 98 | 269 | 364 | 6.48e-20 | 82.8 |
| MS.gene39264.t1 | MTR_8g032390 | 51.648 | 91 | 40 | 3 | 3 | 92 | 282 | 369 | 6.58e-20 | 82.4 |
| MS.gene39264.t1 | MTR_8g433030 | 51.648 | 91 | 40 | 3 | 3 | 92 | 249 | 336 | 8.17e-20 | 82.0 |
| MS.gene39264.t1 | MTR_4g021785 | 45.361 | 97 | 49 | 2 | 3 | 98 | 262 | 355 | 3.39e-19 | 80.5 |
| MS.gene39264.t1 | MTR_5g070088 | 48.352 | 91 | 45 | 2 | 3 | 92 | 290 | 379 | 6.05e-19 | 80.1 |
| MS.gene39264.t1 | MTR_7g079400 | 41.748 | 103 | 53 | 3 | 1 | 98 | 237 | 337 | 1.14e-18 | 79.0 |
| MS.gene39264.t1 | MTR_4g023070 | 42.453 | 106 | 45 | 3 | 4 | 96 | 266 | 368 | 2.18e-18 | 78.2 |
| MS.gene39264.t1 | MTR_4g023190 | 46.465 | 99 | 43 | 5 | 3 | 96 | 264 | 357 | 6.88e-18 | 77.0 |
| MS.gene39264.t1 | MTR_4g023090 | 41.818 | 110 | 45 | 4 | 4 | 97 | 279 | 385 | 7.97e-18 | 77.0 |
| MS.gene39264.t1 | MTR_7g079370 | 46.316 | 95 | 46 | 3 | 1 | 93 | 260 | 351 | 2.87e-17 | 75.1 |
| MS.gene39264.t1 | MTR_8g102520 | 44.211 | 95 | 48 | 3 | 6 | 98 | 231 | 322 | 4.51e-17 | 74.3 |
| MS.gene39264.t1 | MTR_3g034160 | 49.485 | 97 | 46 | 2 | 2 | 97 | 280 | 374 | 6.71e-17 | 74.3 |
| MS.gene39264.t1 | MTR_4g023150 | 44.444 | 99 | 45 | 5 | 3 | 96 | 265 | 358 | 8.10e-17 | 73.9 |
| MS.gene39264.t1 | MTR_4g023120 | 39.450 | 109 | 44 | 3 | 4 | 96 | 266 | 368 | 1.10e-16 | 73.6 |
| MS.gene39264.t1 | MTR_3g034130 | 48.837 | 86 | 34 | 3 | 4 | 84 | 282 | 362 | 1.32e-16 | 73.6 |
| MS.gene39264.t1 | MTR_4g114700 | 43.678 | 87 | 46 | 2 | 6 | 89 | 132 | 218 | 3.57e-16 | 70.9 |
| MS.gene39264.t1 | MTR_7g079450 | 39.175 | 97 | 58 | 1 | 2 | 98 | 42 | 137 | 4.16e-16 | 68.6 |
| MS.gene39264.t1 | MTR_4g023200 | 44.444 | 99 | 45 | 5 | 3 | 96 | 265 | 358 | 7.37e-16 | 71.2 |
| MS.gene39264.t1 | MTR_7g117720 | 42.708 | 96 | 51 | 3 | 2 | 93 | 177 | 272 | 1.89e-15 | 69.3 |
| MS.gene39264.t1 | MTR_8g013005 | 55.000 | 60 | 26 | 1 | 39 | 98 | 1 | 59 | 5.76e-15 | 63.5 |
| MS.gene39264.t1 | MTR_7g079420 | 44.792 | 96 | 46 | 4 | 6 | 98 | 1053 | 1144 | 6.32e-15 | 68.9 |
| MS.gene39264.t1 | MTR_4g023100 | 51.786 | 56 | 24 | 1 | 42 | 97 | 66 | 118 | 4.77e-14 | 63.2 |
| MS.gene39264.t1 | MTR_4g122860 | 42.708 | 96 | 46 | 4 | 8 | 98 | 229 | 320 | 5.80e-14 | 65.9 |
| MS.gene39264.t1 | MTR_4g023430 | 40.000 | 85 | 40 | 3 | 21 | 98 | 142 | 222 | 1.32e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGACGATACGGTTGATATA+TGG | 0.294104 | 1.1:+43114392 | MS.gene39264:CDS |
| ACTACTTATGATACCGTTCA+TGG | 0.348788 | 1.1:+43114466 | MS.gene39264:CDS |
| CGACGATACGGTTGATATAT+GGG | 0.366063 | 1.1:+43114393 | MS.gene39264:CDS |
| TTAAGCTTCCTCATTGTCAC+TGG | 0.388657 | 1.1:-43114659 | None:intergenic |
| TATTGCAAAGATTCACGAAA+TGG | 0.390999 | 1.1:+43114595 | MS.gene39264:CDS |
| TCGTGAATCTTTGCAATAGT+AGG | 0.398959 | 1.1:-43114590 | None:intergenic |
| AATACAAAGTGCAGTCATCT+TGG | 0.401037 | 1.1:+43114425 | MS.gene39264:CDS |
| ATTCCGATTGGCTATGTATA+CGG | 0.410168 | 1.1:+43114618 | MS.gene39264:CDS |
| GTTGGAACAGATTGTCGTAC+TGG | 0.465450 | 1.1:+43114526 | MS.gene39264:CDS |
| ATACAAAGTGCAGTCATCTT+GGG | 0.475310 | 1.1:+43114426 | MS.gene39264:CDS |
| TAAGCTTCCTCATTGTCACT+GGG | 0.487694 | 1.1:-43114658 | None:intergenic |
| GGTTATGGTGGACGACGATA+CGG | 0.513716 | 1.1:+43114381 | None:intergenic |
| TTCACGAAATGGATTCCGAT+TGG | 0.548703 | 1.1:+43114606 | MS.gene39264:CDS |
| ACTCCGTATACATAGCCAAT+CGG | 0.565695 | 1.1:-43114621 | None:intergenic |
| AACAGATTGTCGTACTGGAT+TGG | 0.570980 | 1.1:+43114531 | MS.gene39264:CDS |
| TTCACTCCCCAGTGACAATG+AGG | 0.603016 | 1.1:+43114651 | MS.gene39264:CDS |
| TACAAGGGAAATGCCATGAA+CGG | 0.628081 | 1.1:-43114479 | None:intergenic |
| TTGGTGAGATATGATGAGGA+AGG | 0.631695 | 1.1:+43114550 | MS.gene39264:CDS |
| AAGCTTCCTCATTGTCACTG+GGG | 0.671054 | 1.1:-43114657 | None:intergenic |
| TGGATTGGTGAGATATGATG+AGG | 0.686473 | 1.1:+43114546 | MS.gene39264:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAAAAGTGGTGATATTGT+TGG | + | chr1.1:43114508-43114527 | MS.gene39264:CDS | 30.0% | |
| TATTGCAAAGATTCACGAAA+TGG | + | chr1.1:43114595-43114614 | MS.gene39264:CDS | 30.0% | |
| ! | ACTTTTTGTGCAACATACAA+GGG | - | chr1.1:43114497-43114516 | None:intergenic | 30.0% |
| AATACAAAGTGCAGTCATCT+TGG | + | chr1.1:43114425-43114444 | MS.gene39264:CDS | 35.0% | |
| ACTACTTATGATACCGTTCA+TGG | + | chr1.1:43114466-43114485 | MS.gene39264:CDS | 35.0% | |
| ATACAAAGTGCAGTCATCTT+GGG | + | chr1.1:43114426-43114445 | MS.gene39264:CDS | 35.0% | |
| CTTGTATGTTGCACAAAAAG+TGG | + | chr1.1:43114496-43114515 | MS.gene39264:CDS | 35.0% | |
| TCGTGAATCTTTGCAATAGT+AGG | - | chr1.1:43114593-43114612 | None:intergenic | 35.0% | |
| ! | ATTCCGATTGGCTATGTATA+CGG | + | chr1.1:43114618-43114637 | MS.gene39264:CDS | 35.0% |
| !! | CACTTTTTGTGCAACATACA+AGG | - | chr1.1:43114498-43114517 | None:intergenic | 35.0% |
| AACAGATTGTCGTACTGGAT+TGG | + | chr1.1:43114531-43114550 | MS.gene39264:CDS | 40.0% | |
| ACTCCGTATACATAGCCAAT+CGG | - | chr1.1:43114624-43114643 | None:intergenic | 40.0% | |
| TAAGCTTCCTCATTGTCACT+GGG | - | chr1.1:43114661-43114680 | None:intergenic | 40.0% | |
| TACAAGGGAAATGCCATGAA+CGG | - | chr1.1:43114482-43114501 | None:intergenic | 40.0% | |
| TTAAGCTTCCTCATTGTCAC+TGG | - | chr1.1:43114662-43114681 | None:intergenic | 40.0% | |
| TTGGTGAGATATGATGAGGA+AGG | + | chr1.1:43114550-43114569 | MS.gene39264:CDS | 40.0% | |
| ! | ACGACGATACGGTTGATATA+TGG | + | chr1.1:43114392-43114411 | MS.gene39264:CDS | 40.0% |
| ! | CGACGATACGGTTGATATAT+GGG | + | chr1.1:43114393-43114412 | MS.gene39264:CDS | 40.0% |
| ! | TTCACGAAATGGATTCCGAT+TGG | + | chr1.1:43114606-43114625 | MS.gene39264:CDS | 40.0% |
| !! | TGGATTGGTGAGATATGATG+AGG | + | chr1.1:43114546-43114565 | MS.gene39264:CDS | 40.0% |
| AAGCTTCCTCATTGTCACTG+GGG | - | chr1.1:43114660-43114679 | None:intergenic | 45.0% | |
| GTTGGAACAGATTGTCGTAC+TGG | + | chr1.1:43114526-43114545 | MS.gene39264:CDS | 45.0% | |
| TTCACTCCCCAGTGACAATG+AGG | + | chr1.1:43114651-43114670 | MS.gene39264:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 43114385 | 43114681 | 43114385 | ID=MS.gene39264 |
| chr1.1 | mRNA | 43114385 | 43114681 | 43114385 | ID=MS.gene39264.t1;Parent=MS.gene39264 |
| chr1.1 | exon | 43114385 | 43114681 | 43114385 | ID=MS.gene39264.t1.exon1;Parent=MS.gene39264.t1 |
| chr1.1 | CDS | 43114385 | 43114681 | 43114385 | ID=cds.MS.gene39264.t1;Parent=MS.gene39264.t1 |
| Gene Sequence |
| Protein sequence |
