Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39405.t1 | XP_003608189.2 | 98.9 | 93 | 1 | 0 | 64 | 156 | 63 | 155 | 2.50E-26 | 128.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39405.t1 | G7JQS4 | 98.9 | 93 | 1 | 0 | 64 | 156 | 63 | 155 | 1.8e-26 | 128.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050437 | MS.gene39405 | 0.84541 | 4.13E-59 | -1.69E-46 |
| MS.gene050752 | MS.gene39405 | 0.812246 | 4.67E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene39405 | 0.806026 | 1.00E-49 | -1.69E-46 |
| MS.gene05364 | MS.gene39405 | 0.839308 | 1.71E-57 | -1.69E-46 |
| MS.gene054403 | MS.gene39405 | 0.80978 | 1.60E-50 | -1.69E-46 |
| MS.gene057694 | MS.gene39405 | 0.812433 | 4.25E-51 | -1.69E-46 |
| MS.gene060244 | MS.gene39405 | 0.808448 | 3.08E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene39405 | 0.802663 | 5.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39405.t1 | MTR_4g090520 | 98.077 | 156 | 2 | 1 | 1 | 156 | 1 | 155 | 3.18e-102 | 290 |
| MS.gene39405.t1 | MTR_2g012520 | 87.097 | 62 | 8 | 0 | 95 | 156 | 98 | 159 | 1.01e-26 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39405.t1 | AT4G21620 | 62.963 | 108 | 36 | 3 | 49 | 156 | 28 | 131 | 7.69e-18 | 75.1 |
| MS.gene39405.t1 | AT4G21620 | 78.947 | 57 | 12 | 0 | 100 | 156 | 42 | 98 | 2.05e-17 | 73.2 |
| MS.gene39405.t1 | AT1G61255 | 47.154 | 123 | 47 | 4 | 36 | 156 | 37 | 143 | 8.07e-16 | 70.1 |
Find 61 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATTATGCAGGTGGATA+TGG | 0.240196 | 4.2:+21626043 | MS.gene39405:CDS |
| GGTGGATATGGAGGTGGATA+TGG | 0.281988 | 4.2:+21626055 | MS.gene39405:CDS |
| TATGGTGGCTCCTTTGGATT+TGG | 0.284103 | 4.2:+21625965 | MS.gene39405:CDS |
| CTGGACAGGTGACCTTCTTC+TGG | 0.290223 | 4.2:-21626153 | None:intergenic |
| GGCTACGGTGGTGGCGGTGG+AGG | 0.319831 | 4.2:+21626211 | MS.gene39405:CDS |
| AATGACTATGGTGGCTCCTT+TGG | 0.327553 | 4.2:+21625959 | MS.gene39405:CDS |
| TACGGTGGTGGCGGTGGAGG+TGG | 0.331710 | 4.2:+21626214 | MS.gene39405:CDS |
| GTTGGTGGTGGCAATGACTA+TGG | 0.362645 | 4.2:+21625947 | MS.gene39405:CDS |
| AAGGGCTACGGTGGTGGCGG+TGG | 0.371027 | 4.2:+21626208 | MS.gene39405:CDS |
| GCAAGATGGTGAGCAAAATA+AGG | 0.379174 | 4.2:-21625829 | None:intergenic |
| TTTGTAGAGTGCCCTCCATT+TGG | 0.386250 | 4.2:-21626082 | None:intergenic |
| GGTGGTGGCGGTGGAGGTGG+TGG | 0.408190 | 4.2:+21626217 | MS.gene39405:CDS |
| GTCAGCAGATGGTGAAGGTT+TGG | 0.409569 | 4.2:-21625897 | None:intergenic |
| GCCACCACCGTAGCCCTTGC+CGG | 0.412928 | 4.2:-21626203 | None:intergenic |
| TCCGGCAAGGGCTACGGTGG+TGG | 0.414517 | 4.2:+21626202 | MS.gene39405:CDS |
| AAGGAAGTAAAACACCTTGC+TGG | 0.414681 | 4.2:-21626172 | None:intergenic |
| GGCTCCTTTGGATTTGGACC+AGG | 0.449384 | 4.2:+21625971 | MS.gene39405:CDS |
| GGTGGATATGGTGGTCCAAA+TGG | 0.453271 | 4.2:+21626067 | MS.gene39405:CDS |
| TTCCTTCAGTCGCTCCGGCA+AGG | 0.458448 | 4.2:+21626189 | MS.gene39405:CDS |
| TTTGGATTTGGACCAGGAGG+AGG | 0.459507 | 4.2:+21625977 | MS.gene39405:CDS |
| AGTAAAACACCTTGCTGGAC+AGG | 0.461202 | 4.2:-21626167 | None:intergenic |
| TCCTTCAGTCGCTCCGGCAA+GGG | 0.470860 | 4.2:+21626190 | MS.gene39405:CDS |
| AATGTACTGCTTACTGTTGA+GGG | 0.477371 | 4.2:+21626263 | MS.gene39405:CDS |
| CTGTCTTTGCAGACAACAGT+TGG | 0.480004 | 4.2:-21626118 | None:intergenic |
| AGTCGCTCCGGCAAGGGCTA+CGG | 0.483160 | 4.2:+21626196 | MS.gene39405:CDS |
| GGCAAGGGCTACGGTGGTGG+CGG | 0.485399 | 4.2:+21626205 | MS.gene39405:CDS |
| TGAAGGTTTGGTTGTTGATG+TGG | 0.499325 | 4.2:-21625885 | None:intergenic |
| TCCTTTGGATTTGGACCAGG+AGG | 0.509727 | 4.2:+21625974 | MS.gene39405:CDS |
| AAATGTACTGCTTACTGTTG+AGG | 0.516007 | 4.2:+21626262 | MS.gene39405:CDS |
| GTGACCTTCTTCTGGTAACA+AGG | 0.517811 | 4.2:-21626145 | None:intergenic |
| AATGGAGGGCACTCTACAAA+TGG | 0.522558 | 4.2:+21626085 | MS.gene39405:CDS |
| AACAACAACAATAACAAAGA+TGG | 0.524444 | 4.2:+21625923 | MS.gene39405:CDS |
| TTGTTGTTGTTGTCAGCAGA+TGG | 0.534850 | 4.2:-21625908 | None:intergenic |
| GACAACAACATCATAGGAGG+AGG | 0.541463 | 4.2:+21626019 | MS.gene39405:CDS |
| GGATATGGTGGTCCAAATGG+AGG | 0.547027 | 4.2:+21626070 | MS.gene39405:CDS |
| TTTACTTCCTTCAGTCGCTC+CGG | 0.547199 | 4.2:+21626184 | MS.gene39405:CDS |
| GCCCTTGCCGGAGCGACTGA+AGG | 0.553888 | 4.2:-21626191 | None:intergenic |
| CGCTCCGGCAAGGGCTACGG+TGG | 0.556652 | 4.2:+21626199 | MS.gene39405:CDS |
| AACAAAGATGGTGTTGGTGG+TGG | 0.557994 | 4.2:+21625935 | MS.gene39405:CDS |
| AACAATAACAAAGATGGTGT+TGG | 0.562868 | 4.2:+21625929 | MS.gene39405:CDS |
| TATGCAGGTGGATATGGAGG+TGG | 0.563930 | 4.2:+21626049 | MS.gene39405:CDS |
| GGTAGTAACTGTTAGCAAGA+TGG | 0.568446 | 4.2:-21625843 | None:intergenic |
| AATTATGCAGGTGGATATGG+AGG | 0.570541 | 4.2:+21626046 | MS.gene39405:CDS |
| AGGCCCTTGTTACCAGAAGA+AGG | 0.575423 | 4.2:+21626141 | MS.gene39405:CDS |
| GATATGGTGGTCCAAATGGA+GGG | 0.580834 | 4.2:+21626071 | MS.gene39405:CDS |
| AACAACATCATAGGAGGAGG+GGG | 0.583321 | 4.2:+21626022 | MS.gene39405:CDS |
| TTGTTGTCAGCAGATGGTGA+AGG | 0.584431 | 4.2:-21625902 | None:intergenic |
| TCCTCCTGGTCCAAATCCAA+AGG | 0.585947 | 4.2:-21625975 | None:intergenic |
| GGTATGCTAAAACCTCCTCC+TGG | 0.595051 | 4.2:-21625989 | None:intergenic |
| CAACAACATCATAGGAGGAG+GGG | 0.596964 | 4.2:+21626021 | MS.gene39405:CDS |
| GGATATGGAGGTGGATATGG+TGG | 0.597908 | 4.2:+21626058 | MS.gene39405:CDS |
| TTTGACAACAACATCATAGG+AGG | 0.619262 | 4.2:+21626016 | MS.gene39405:CDS |
| ATGATGTTGTTGTCAAAACC+TGG | 0.622315 | 4.2:-21626010 | None:intergenic |
| AATAACAAAGATGGTGTTGG+TGG | 0.624778 | 4.2:+21625932 | MS.gene39405:CDS |
| ACAACAACATCATAGGAGGA+GGG | 0.631371 | 4.2:+21626020 | MS.gene39405:CDS |
| AGAAGGTCACCTGTCCAGCA+AGG | 0.643768 | 4.2:+21626158 | MS.gene39405:CDS |
| GGTGGTGGCAATGACTATGG+TGG | 0.659694 | 4.2:+21625950 | MS.gene39405:CDS |
| TGACCTTCTTCTGGTAACAA+GGG | 0.660322 | 4.2:-21626144 | None:intergenic |
| ACTGTTGTCTGCAAAGACAG+AGG | 0.691072 | 4.2:+21626121 | MS.gene39405:CDS |
| TGTTGATGTGGATCTGCGAG+AGG | 0.712516 | 4.2:-21625873 | None:intergenic |
| GGATCTGCGAGAGGACACAG+AGG | 0.788957 | 4.2:-21625864 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAACAACAATAACAAAGA+TGG | + | chr4.2:21625923-21625942 | MS.gene39405:CDS | 25.0% |
| !! | ACATTTCTTTTTGCAATCAA+TGG | - | chr4.2:21626248-21626267 | None:intergenic | 25.0% |
| AACAATAACAAAGATGGTGT+TGG | + | chr4.2:21625929-21625948 | MS.gene39405:CDS | 30.0% | |
| ATGATGTTGTTGTCAAAACC+TGG | - | chr4.2:21626013-21626032 | None:intergenic | 35.0% | |
| TTTGACAACAACATCATAGG+AGG | + | chr4.2:21626016-21626035 | MS.gene39405:CDS | 35.0% | |
| ! | AATAACAAAGATGGTGTTGG+TGG | + | chr4.2:21625932-21625951 | MS.gene39405:CDS | 35.0% |
| !! | GGTTTTGACAACAACATCAT+AGG | + | chr4.2:21626013-21626032 | MS.gene39405:CDS | 35.0% |
| AAGGAAGTAAAACACCTTGC+TGG | - | chr4.2:21626175-21626194 | None:intergenic | 40.0% | |
| AATTATGCAGGTGGATATGG+AGG | + | chr4.2:21626046-21626065 | MS.gene39405:CDS | 40.0% | |
| ACAACAACATCATAGGAGGA+GGG | + | chr4.2:21626020-21626039 | MS.gene39405:CDS | 40.0% | |
| GCAAGATGGTGAGCAAAATA+AGG | - | chr4.2:21625832-21625851 | None:intergenic | 40.0% | |
| GGAAATTATGCAGGTGGATA+TGG | + | chr4.2:21626043-21626062 | MS.gene39405:CDS | 40.0% | |
| GGTAGTAACTGTTAGCAAGA+TGG | - | chr4.2:21625846-21625865 | None:intergenic | 40.0% | |
| TGACCTTCTTCTGGTAACAA+GGG | - | chr4.2:21626147-21626166 | None:intergenic | 40.0% | |
| ! | TGAAGGTTTGGTTGTTGATG+TGG | - | chr4.2:21625888-21625907 | None:intergenic | 40.0% |
| ! | TTGTTGTTGTTGTCAGCAGA+TGG | - | chr4.2:21625911-21625930 | None:intergenic | 40.0% |
| AACAACATCATAGGAGGAGG+GGG | + | chr4.2:21626022-21626041 | MS.gene39405:CDS | 45.0% | |
| AATGACTATGGTGGCTCCTT+TGG | + | chr4.2:21625959-21625978 | MS.gene39405:CDS | 45.0% | |
| ACTGTTGTCTGCAAAGACAG+AGG | + | chr4.2:21626121-21626140 | MS.gene39405:CDS | 45.0% | |
| AGTAAAACACCTTGCTGGAC+AGG | - | chr4.2:21626170-21626189 | None:intergenic | 45.0% | |
| CAACAACATCATAGGAGGAG+GGG | + | chr4.2:21626021-21626040 | MS.gene39405:CDS | 45.0% | |
| CTGTCTTTGCAGACAACAGT+TGG | - | chr4.2:21626121-21626140 | None:intergenic | 45.0% | |
| GACAACAACATCATAGGAGG+AGG | + | chr4.2:21626019-21626038 | MS.gene39405:CDS | 45.0% | |
| GATATGGTGGTCCAAATGGA+GGG | + | chr4.2:21626071-21626090 | MS.gene39405:CDS | 45.0% | |
| GTGACCTTCTTCTGGTAACA+AGG | - | chr4.2:21626148-21626167 | None:intergenic | 45.0% | |
| TATGGTGGCTCCTTTGGATT+TGG | + | chr4.2:21625965-21625984 | MS.gene39405:CDS | 45.0% | |
| TTGTTGTCAGCAGATGGTGA+AGG | - | chr4.2:21625905-21625924 | None:intergenic | 45.0% | |
| TTTACTTCCTTCAGTCGCTC+CGG | + | chr4.2:21626184-21626203 | MS.gene39405:CDS | 45.0% | |
| ! | TTTGTAGAGTGCCCTCCATT+TGG | - | chr4.2:21626085-21626104 | None:intergenic | 45.0% |
| !! | AACAAAGATGGTGTTGGTGG+TGG | + | chr4.2:21625935-21625954 | MS.gene39405:CDS | 45.0% |
| !! | AATGGAGGGCACTCTACAAA+TGG | + | chr4.2:21626085-21626104 | MS.gene39405:CDS | 45.0% |
| AGGCCCTTGTTACCAGAAGA+AGG | + | chr4.2:21626141-21626160 | MS.gene39405:CDS | 50.0% | |
| GGATATGGAGGTGGATATGG+TGG | + | chr4.2:21626058-21626077 | MS.gene39405:CDS | 50.0% | |
| GGATATGGTGGTCCAAATGG+AGG | + | chr4.2:21626070-21626089 | MS.gene39405:CDS | 50.0% | |
| GGTATGCTAAAACCTCCTCC+TGG | - | chr4.2:21625992-21626011 | None:intergenic | 50.0% | |
| GGTGGATATGGAGGTGGATA+TGG | + | chr4.2:21626055-21626074 | MS.gene39405:CDS | 50.0% | |
| GGTGGATATGGTGGTCCAAA+TGG | + | chr4.2:21626067-21626086 | MS.gene39405:CDS | 50.0% | |
| GTCAGCAGATGGTGAAGGTT+TGG | - | chr4.2:21625900-21625919 | None:intergenic | 50.0% | |
| TATGCAGGTGGATATGGAGG+TGG | + | chr4.2:21626049-21626068 | MS.gene39405:CDS | 50.0% | |
| TCCTCCTGGTCCAAATCCAA+AGG | - | chr4.2:21625978-21625997 | None:intergenic | 50.0% | |
| TCCTTTGGATTTGGACCAGG+AGG | + | chr4.2:21625974-21625993 | MS.gene39405:CDS | 50.0% | |
| TGTTGATGTGGATCTGCGAG+AGG | - | chr4.2:21625876-21625895 | None:intergenic | 50.0% | |
| TTTGGATTTGGACCAGGAGG+AGG | + | chr4.2:21625977-21625996 | MS.gene39405:CDS | 50.0% | |
| !! | GGAGGAGGTTTTAGCATACC+AGG | + | chr4.2:21625992-21626011 | MS.gene39405:CDS | 50.0% |
| !! | GTTGGTGGTGGCAATGACTA+TGG | + | chr4.2:21625947-21625966 | MS.gene39405:CDS | 50.0% |
| AGAAGGTCACCTGTCCAGCA+AGG | + | chr4.2:21626158-21626177 | MS.gene39405:CDS | 55.0% | |
| CTGGACAGGTGACCTTCTTC+TGG | - | chr4.2:21626156-21626175 | None:intergenic | 55.0% | |
| GGAGGAGGGGGAAATTATGC+AGG | + | chr4.2:21626034-21626053 | MS.gene39405:CDS | 55.0% | |
| GGAGGGGGAAATTATGCAGG+TGG | + | chr4.2:21626037-21626056 | MS.gene39405:CDS | 55.0% | |
| GGCTCCTTTGGATTTGGACC+AGG | + | chr4.2:21625971-21625990 | MS.gene39405:CDS | 55.0% | |
| ! | GGTGGTGGCAATGACTATGG+TGG | + | chr4.2:21625950-21625969 | MS.gene39405:CDS | 55.0% |
| GGATCTGCGAGAGGACACAG+AGG | - | chr4.2:21625867-21625886 | None:intergenic | 60.0% | |
| TCCTTCAGTCGCTCCGGCAA+GGG | + | chr4.2:21626190-21626209 | MS.gene39405:CDS | 60.0% | |
| TTCCTTCAGTCGCTCCGGCA+AGG | + | chr4.2:21626189-21626208 | MS.gene39405:CDS | 60.0% | |
| AGTCGCTCCGGCAAGGGCTA+CGG | + | chr4.2:21626196-21626215 | MS.gene39405:CDS | 65.0% | |
| GCCACCACCGTAGCCCTTGC+CGG | - | chr4.2:21626206-21626225 | None:intergenic | 70.0% | |
| GCCCTTGCCGGAGCGACTGA+AGG | - | chr4.2:21626194-21626213 | None:intergenic | 70.0% | |
| TCCGGCAAGGGCTACGGTGG+TGG | + | chr4.2:21626202-21626221 | MS.gene39405:CDS | 70.0% | |
| ! | GGCAAGGGCTACGGTGGTGG+CGG | + | chr4.2:21626205-21626224 | MS.gene39405:CDS | 70.0% |
| !! | AAGGGCTACGGTGGTGGCGG+TGG | + | chr4.2:21626208-21626227 | MS.gene39405:CDS | 70.0% |
| !! | TACGGTGGTGGCGGTGGAGG+TGG | + | chr4.2:21626214-21626233 | MS.gene39405:CDS | 70.0% |
| CGCTCCGGCAAGGGCTACGG+TGG | + | chr4.2:21626199-21626218 | MS.gene39405:CDS | 75.0% | |
| !! | GGCTACGGTGGTGGCGGTGG+AGG | + | chr4.2:21626211-21626230 | MS.gene39405:CDS | 75.0% |
| !! | GGTGGTGGCGGTGGAGGTGG+TGG | + | chr4.2:21626217-21626236 | MS.gene39405:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 21625812 | 21626282 | 21625812 | ID=MS.gene39405 |
| chr4.2 | mRNA | 21625812 | 21626282 | 21625812 | ID=MS.gene39405.t1;Parent=MS.gene39405 |
| chr4.2 | exon | 21625812 | 21626282 | 21625812 | ID=MS.gene39405.t1.exon1;Parent=MS.gene39405.t1 |
| chr4.2 | CDS | 21625812 | 21626282 | 21625812 | ID=cds.MS.gene39405.t1;Parent=MS.gene39405.t1 |
| Gene Sequence |
| Protein sequence |
