AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050437


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050437.t1 MTR_7g109980 96.667 120 4 0 1 120 1 120 2.55e-81 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050437.t1 AT4G01897 63.248 117 43 0 3 119 5 121 2.97e-55 168

Find 24 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGATCTAGTTTGCTAAGA+TGG 0.325847 7.1:+4611843 None:intergenic
CAAAGAAATGGGTCCACTTT+CGG 0.360708 7.1:-4611896 MS.gene050437:CDS
TCCTCAGCTTGGCGATGGTT+TGG 0.387394 7.1:-4610600 MS.gene050437:intron
TGGGAAGAATTGCAAAGAAA+TGG 0.392074 7.1:-4611908 MS.gene050437:CDS
TCTTCAGATTGATGCTAAGA+AGG 0.429001 7.1:-4611636 MS.gene050437:intron
CCATCTTAGCAAACTAGATC+AGG 0.463319 7.1:-4611843 MS.gene050437:intron
GTCAAGTTCACCACCGAAAG+TGG 0.465358 7.1:+4611883 None:intergenic
CTATTTCCTCAGCTTGGCGA+TGG 0.484872 7.1:-4610605 MS.gene050437:intron
AATCAGCACGGCGAAGGAAT+GGG 0.486850 7.1:-4611927 MS.gene050437:CDS
GGGAAGAATTGCAAAGAAAT+GGG 0.488668 7.1:-4611907 MS.gene050437:CDS
GAATCAGCACGGCGAAGGAA+TGG 0.494049 7.1:-4611928 MS.gene050437:CDS
GAGAAGCTGTCTCTTTCAAA+TGG 0.500982 7.1:-4610482 MS.gene050437:CDS
AGCTGTCTCTTTCAAATGGT+CGG 0.507865 7.1:-4610478 MS.gene050437:CDS
TCTTAGCATCAATCTGAAGA+AGG 0.553114 7.1:+4611639 None:intergenic
TGGTTTGGTGTATGAAATTG+TGG 0.564813 7.1:-4610585 MS.gene050437:CDS
GTGACCACATCTAGAGAGAG+AGG 0.580003 7.1:+4610512 None:intergenic
GTACAGAATCAGCACGGCGA+AGG 0.581959 7.1:-4611933 MS.gene050437:CDS
ATTGCAGGTTCGATCAACGT+TGG 0.585116 7.1:-4611696 MS.gene050437:intron
TATCCCTCTCTCTCTAGATG+TGG 0.592426 7.1:-4610516 MS.gene050437:CDS
TTGGTGTATGAAATTGTGGA+CGG 0.638694 7.1:-4610581 MS.gene050437:CDS
AGAAATGGGTCCACTTTCGG+TGG 0.639639 7.1:-4611893 MS.gene050437:CDS
TCTAGATGTGGTCACAAAAG+CGG 0.643197 7.1:-4610504 MS.gene050437:CDS
AGAGGGATAAAGCTTCGAGA+AGG 0.643890 7.1:+4610530 None:intergenic
TGACCACATCTAGAGAGAGA+GGG 0.654116 7.1:+4610513 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTATTTTATTATTGC+AGG - chr7.1:4610690-4610709 MS.gene050437:intron 10.0%
!! CACAGTTATTTATTATTATT+AGG - chr7.1:4611713-4611732 MS.gene050437:intron 15.0%
!! TAATTAAAAAACATGCTTAT+TGG - chr7.1:4610853-4610872 MS.gene050437:intron 15.0%
!!! GTTTCTTTTACTATAAATAT+AGG + chr7.1:4611379-4611398 None:intergenic 15.0%
!!! ATTTTTCAGAAACGTATTTT+TGG + chr7.1:4611428-4611447 None:intergenic 20.0%
!!! CAGATTTGTTACAATTTTAT+GGG + chr7.1:4611403-4611422 None:intergenic 20.0%
!!! TAAAAAAAAGCATAGTGAAA+GGG + chr7.1:4610804-4610823 None:intergenic 20.0%
!!! TTAAAAAAAAGCATAGTGAA+AGG + chr7.1:4610805-4610824 None:intergenic 20.0%
!!! TTTTTCAGAAACGTATTTTT+GGG + chr7.1:4611427-4611446 None:intergenic 20.0%
! CCATAAAATTGTAACAAATC+TGG - chr7.1:4611401-4611420 MS.gene050437:intron 25.0%
! GTTCTATTCACTTTGATTAT+TGG + chr7.1:4610977-4610996 None:intergenic 25.0%
! TTGATTCAACTAAAATCTGA+AGG - chr7.1:4611614-4611633 MS.gene050437:intron 25.0%
!! TTTCAGCAAACATCAATTTT+TGG - chr7.1:4611550-4611569 MS.gene050437:intron 25.0%
!!! CCAGATTTGTTACAATTTTA+TGG + chr7.1:4611404-4611423 None:intergenic 25.0%
!!! TATTAGAAAAGTATCTGTTG+CGG - chr7.1:4610889-4610908 MS.gene050437:intron 25.0%
AAAATAACAGCACGCAAAAT+CGG - chr7.1:4611443-4611462 MS.gene050437:intron 30.0%
TAACGTTACTCATCATATAG+TGG + chr7.1:4611128-4611147 None:intergenic 30.0%
TATAAGTCGCTTCATTCATT+CGG - chr7.1:4610648-4610667 MS.gene050437:intron 30.0%
TTGTATAGAGTTTGTCTCTT+TGG + chr7.1:4611282-4611301 None:intergenic 30.0%
TTGTGAAAGTGACTCAAAAT+TGG + chr7.1:4611586-4611605 None:intergenic 30.0%
! TGTTTGCTGAAAGTACAAAT+CGG + chr7.1:4611542-4611561 None:intergenic 30.0%
! TTACTCATCATATAGTGGAA+CGG + chr7.1:4611123-4611142 None:intergenic 30.0%
!! AATAGCTTCCCTCATTTTTA+TGG - chr7.1:4611847-4611866 MS.gene050437:CDS 30.0%
!! AGTGTAAACTTTGAGATAGA+GGG + chr7.1:4611347-4611366 None:intergenic 30.0%
!! ATAGCTTCCCTCATTTTTAT+GGG - chr7.1:4611848-4611867 MS.gene050437:CDS 30.0%
AAATAACAGCACGCAAAATC+GGG - chr7.1:4611444-4611463 MS.gene050437:intron 35.0%
AAGAATCTGTTTACCCAGTT+CGG - chr7.1:4611061-4611080 MS.gene050437:intron 35.0%
AATCCTAATAGTGTTTCCCA+AGG - chr7.1:4611227-4611246 MS.gene050437:intron 35.0%
ACCAATAACAACTTGATGTG+TGG - chr7.1:4610931-4610950 MS.gene050437:intron 35.0%
ACTATTAGGATTGAGTCTCT+TGG + chr7.1:4611219-4611238 None:intergenic 35.0%
AGAAAGGCAACAACAAAACT+AGG + chr7.1:4611307-4611326 None:intergenic 35.0%
AGTGACTCAAAATTGGAGTT+TGG + chr7.1:4611579-4611598 None:intergenic 35.0%
GAAAGGCAACAACAAAACTA+GGG + chr7.1:4611306-4611325 None:intergenic 35.0%
GAGTAACGTTACAAAAGAGA+TGG - chr7.1:4611137-4611156 MS.gene050437:intron 35.0%
GGGAAGAATTGCAAAGAAAT+GGG - chr7.1:4610494-4610513 MS.gene050437:CDS 35.0%
TCTTAGCATCAATCTGAAGA+AGG + chr7.1:4610765-4610784 None:intergenic 35.0%
TCTTCAGATTGATGCTAAGA+AGG - chr7.1:4610765-4610784 MS.gene050437:intron 35.0%
TGGGAAGAATTGCAAAGAAA+TGG - chr7.1:4610493-4610512 MS.gene050437:CDS 35.0%
TTGGTGTATGAAATTGTGGA+CGG - chr7.1:4611820-4611839 MS.gene050437:intron 35.0%
! CAGTTTGGTACTGGAAAAAT+CGG + chr7.1:4611493-4611512 None:intergenic 35.0%
! GTTTGCTGAAAGTACAAATC+GGG + chr7.1:4611541-4611560 None:intergenic 35.0%
! TCCACACATCAAGTTGTTAT+TGG + chr7.1:4610935-4610954 None:intergenic 35.0%
! TTTGCTGAAAGTACAAATCG+GGG + chr7.1:4611540-4611559 None:intergenic 35.0%
! TTTTGTGTACAGAATCAGCA+CGG - chr7.1:4610462-4610481 MS.gene050437:CDS 35.0%
!! CAGTGTAAACTTTGAGATAG+AGG + chr7.1:4611348-4611367 None:intergenic 35.0%
!! GTGTAAACTTTGAGATAGAG+GGG + chr7.1:4611346-4611365 None:intergenic 35.0%
!! TGGTTTGGTGTATGAAATTG+TGG - chr7.1:4611816-4611835 MS.gene050437:intron 35.0%
!! TGTAAACTTTGAGATAGAGG+GGG + chr7.1:4611345-4611364 None:intergenic 35.0%
!!! GCTGTGTTGTTTTCTACTTT+TGG + chr7.1:4611015-4611034 None:intergenic 35.0%
!!! TTTTGAGGTTGATTCACCTT+GGG + chr7.1:4611246-4611265 None:intergenic 35.0%
AGCTGTCTCTTTCAAATGGT+CGG - chr7.1:4611923-4611942 MS.gene050437:CDS 40.0%
AGGATTGAGTCTCTTGGTTA+CGG + chr7.1:4611213-4611232 None:intergenic 40.0%
CAAAGAAATGGGTCCACTTT+CGG - chr7.1:4610505-4610524 MS.gene050437:CDS 40.0%
CCATCTTAGCAAACTAGATC+AGG - chr7.1:4610558-4610577 MS.gene050437:CDS 40.0%
GACTCAAAATTGGAGTTTGG+AGG + chr7.1:4611576-4611595 None:intergenic 40.0%
GAGAAGCTGTCTCTTTCAAA+TGG - chr7.1:4611919-4611938 MS.gene050437:CDS 40.0%
GAGAAGGCCCATAAAAATGA+GGG + chr7.1:4611858-4611877 None:intergenic 40.0%
GCTGAAACAAGGGTTTAGAA+AGG + chr7.1:4611323-4611342 None:intergenic 40.0%
TCACCTTGGGAAACACTATT+AGG + chr7.1:4611233-4611252 None:intergenic 40.0%
TCTAGATGTGGTCACAAAAG+CGG - chr7.1:4611897-4611916 MS.gene050437:CDS 40.0%
! GGAACAAACACTAGGTTTTG+AGG + chr7.1:4611261-4611280 None:intergenic 40.0%
! TCATCATATAGTGGAACGGA+GGG + chr7.1:4611119-4611138 None:intergenic 40.0%
!! CCTGATCTAGTTTGCTAAGA+TGG + chr7.1:4610561-4610580 None:intergenic 40.0%
!! GTCTCTTTGGAACAAACACT+AGG + chr7.1:4611269-4611288 None:intergenic 40.0%
!!! GTTTTGAGGTTGATTCACCT+TGG + chr7.1:4611247-4611266 None:intergenic 40.0%
AGAGGGATAAAGCTTCGAGA+AGG + chr7.1:4611874-4611893 None:intergenic 45.0%
AGGTCAATTTGGACCGAACT+GGG + chr7.1:4611077-4611096 None:intergenic 45.0%
ATTGCAGGTTCGATCAACGT+TGG - chr7.1:4610705-4610724 MS.gene050437:intron 45.0%
CAACTTGATGTGTGGAGCAA+AGG - chr7.1:4610939-4610958 MS.gene050437:intron 45.0%
CGAGAAGGCCCATAAAAATG+AGG + chr7.1:4611859-4611878 None:intergenic 45.0%
CTCCCAAAGACAGCCAAAAT+CGG - chr7.1:4611513-4611532 MS.gene050437:intron 45.0%
GCTGGGATTTGGGTAGAATT+AGG + chr7.1:4611184-4611203 None:intergenic 45.0%
GGATTGAGTCTCTTGGTTAC+GGG + chr7.1:4611212-4611231 None:intergenic 45.0%
GGATTTGGGTAGAATTAGGC+GGG + chr7.1:4611180-4611199 None:intergenic 45.0%
GGGATTTGGGTAGAATTAGG+CGG + chr7.1:4611181-4611200 None:intergenic 45.0%
GGTCCAAATTGACCTAGTCT+GGG - chr7.1:4611082-4611101 MS.gene050437:intron 45.0%
GTCCAAATTGACCTAGTCTG+GGG - chr7.1:4611083-4611102 MS.gene050437:intron 45.0%
TAGGTCAATTTGGACCGAAC+TGG + chr7.1:4611078-4611097 None:intergenic 45.0%
TATCCCTCTCTCTCTAGATG+TGG - chr7.1:4611885-4611904 MS.gene050437:CDS 45.0%
TCCAAATTGACCTAGTCTGG+GGG - chr7.1:4611084-4611103 MS.gene050437:intron 45.0%
TCCCAAAGACAGCCAAAATC+GGG - chr7.1:4611514-4611533 MS.gene050437:intron 45.0%
TGACCACATCTAGAGAGAGA+GGG + chr7.1:4611891-4611910 None:intergenic 45.0%
! CTCATCATATAGTGGAACGG+AGG + chr7.1:4611120-4611139 None:intergenic 45.0%
!! GCTATGCTATTTCCTCAGCT+TGG - chr7.1:4611790-4611809 MS.gene050437:intron 45.0%
AATCAGCACGGCGAAGGAAT+GGG - chr7.1:4610474-4610493 MS.gene050437:CDS 50.0%
AGAAATGGGTCCACTTTCGG+TGG - chr7.1:4610508-4610527 MS.gene050437:CDS 50.0%
AGATAGAGGGGGCTGAAACA+AGG + chr7.1:4611334-4611353 None:intergenic 50.0%
AGTCTCTTGGTTACGGGAAG+AGG + chr7.1:4611206-4611225 None:intergenic 50.0%
CGCCCGAATGGTAAAAATCG+GGG + chr7.1:4611470-4611489 None:intergenic 50.0%
CGGTCCAAATTGACCTAGTC+TGG - chr7.1:4611081-4611100 MS.gene050437:intron 50.0%
CTATTTCCTCAGCTTGGCGA+TGG - chr7.1:4611796-4611815 MS.gene050437:intron 50.0%
GATAGAGGGGGCTGAAACAA+GGG + chr7.1:4611333-4611352 None:intergenic 50.0%
GCGCCCGAATGGTAAAAATC+GGG + chr7.1:4611471-4611490 None:intergenic 50.0%
GGCGCCCGAATGGTAAAAAT+CGG + chr7.1:4611472-4611491 None:intergenic 50.0%
GTCAAGTTCACCACCGAAAG+TGG + chr7.1:4610521-4610540 None:intergenic 50.0%
GTGACCACATCTAGAGAGAG+AGG + chr7.1:4611892-4611911 None:intergenic 50.0%
TCCCCCAGACTAGGTCAATT+TGG + chr7.1:4611088-4611107 None:intergenic 50.0%
! CTTTGGGAGCAGTTTGGTAC+TGG + chr7.1:4611502-4611521 None:intergenic 50.0%
! GGCCCCGATTTTTACCATTC+GGG - chr7.1:4611465-4611484 MS.gene050437:intron 50.0%
! GGGCCCCGATTTTTACCATT+CGG - chr7.1:4611464-4611483 MS.gene050437:intron 50.0%
! TACAAATCGGGGCCCGATTT+TGG + chr7.1:4611529-4611548 None:intergenic 50.0%
!!! GCCCGATTTTGGCTGTCTTT+GGG + chr7.1:4611518-4611537 None:intergenic 50.0%
ACCAAACCATCGCCAAGCTG+AGG + chr7.1:4611805-4611824 None:intergenic 55.0%
ACGGGAAGAGGCTGGGATTT+GGG + chr7.1:4611194-4611213 None:intergenic 55.0%
CTTGGTTACGGGAAGAGGCT+GGG + chr7.1:4611201-4611220 None:intergenic 55.0%
GAATCAGCACGGCGAAGGAA+TGG - chr7.1:4610473-4610492 MS.gene050437:CDS 55.0%
GTACAGAATCAGCACGGCGA+AGG - chr7.1:4610468-4610487 MS.gene050437:CDS 55.0%
TACGGGAAGAGGCTGGGATT+TGG + chr7.1:4611195-4611214 None:intergenic 55.0%
TCCTCAGCTTGGCGATGGTT+TGG - chr7.1:4611801-4611820 MS.gene050437:intron 55.0%
TCTTGGTTACGGGAAGAGGC+TGG + chr7.1:4611202-4611221 None:intergenic 55.0%
TGGAAAAATCGGCGCCCGAA+TGG + chr7.1:4611482-4611501 None:intergenic 55.0%
! GGCTGTCTTTGGGAGCAGTT+TGG + chr7.1:4611508-4611527 None:intergenic 55.0%
!!! GGCCCGATTTTGGCTGTCTT+TGG + chr7.1:4611519-4611538 None:intergenic 55.0%
GCTGCTCTCTCCCCCAGACT+AGG + chr7.1:4611097-4611116 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 4610454 4611969 4610454 ID=MS.gene050437
chr7.1 mRNA 4610454 4611969 4610454 ID=MS.gene050437.t1;Parent=MS.gene050437
chr7.1 exon 4611844 4611969 4611844 ID=MS.gene050437.t1.exon1;Parent=MS.gene050437.t1
chr7.1 CDS 4611844 4611969 4611844 ID=cds.MS.gene050437.t1;Parent=MS.gene050437.t1
chr7.1 exon 4611637 4611711 4611637 ID=MS.gene050437.t1.exon2;Parent=MS.gene050437.t1
chr7.1 CDS 4611637 4611711 4611637 ID=cds.MS.gene050437.t1;Parent=MS.gene050437.t1
chr7.1 exon 4610454 4610615 4610454 ID=MS.gene050437.t1.exon3;Parent=MS.gene050437.t1
chr7.1 CDS 4610454 4610615 4610454 ID=cds.MS.gene050437.t1;Parent=MS.gene050437.t1
Gene Sequence

>MS.gene050437

ATGAGTGATTTTGTGTACAGAATCAGCACGGCGAAGGAATGGGAAGAATTGCAAAGAAATGGGTCCACTTTCGGTGGTGAACTTGACAAGTCTTCTTCTTTCATCCATCTTAGCAAACTAGATCAGGTTAATTTATTTCTATGTTTTTCTAATATTTTGATTTTGAGTTCATTTCACTGTTTTGCTCATGTTCATATAAGTCGCTTCATTCATTCGGATTTCTGAATTCTGAATTCTATAATTATTTTATTATTGCAGGTTCGATCAACGTTGGATAATTTTTTCTTGAATTCTAAAGATGAACTCTACCTTCTTCAGATTGATGCTAAGAAGGTTTGCTCATACTTCCCTTTCACTATGCTTTTTTTTAATGTGTTTGTGATTTTATTTGTGAATAAATAATTAAAAAACATGCTTATTGGTAGCGATGAAGATTATTAGAAAAGTATCTGTTGCGGATTCGATGAGAAAACTCACACCAATAACAACTTGATGTGTGGAGCAAAGGATAATAAAGCAACCAATAATCAAAGTGAATAGAACAGTTATAATCACAAGCCAAAAGTAGAAAACAACACAGCAAGAGAAAAGAAGAAGAGAGAACACAAAGAATCTGTTTACCCAGTTCGGTCCAAATTGACCTAGTCTGGGGGAGAGAGCAGCCCTCCGTTCCACTATATGATGAGTAACGTTACAAAAGAGATGGATTACAAGAATAAGTCTCCCGCCTAATTCTACCCAAATCCCAGCCTCTTCCCGTAACCAAGAGACTCAATCCTAATAGTGTTTCCCAAGGTGAATCAACCTCAAAACCTAGTGTTTGTTCCAAAGAGACAAACTCTATACAAACCCTAGTTTTGTTGTTGCCTTTCTAAACCCTTGTTTCAGCCCCCTCTATCTCAAAGTTTACACTGATACAATGCCTATATTTATAGTAAAAGAAACTCCCATAAAATTGTAACAAATCTGGCCCAAAAATACGTTTCTGAAAAATAACAGCACGCAAAATCGGGCCCCGATTTTTACCATTCGGGCGCCGATTTTTCCAGTACCAAACTGCTCCCAAAGACAGCCAAAATCGGGCCCCGATTTGTACTTTCAGCAAACATCAATTTTTGGCCTCCAAACTCCAATTTTGAGTCACTTTCACAACAGTATCGTTGATTCAACTAAAATCTGAAGGAAAATGAAACTCTGTTTCTATTCATAAAGTGACACTTTAGTGTGTATTAAAACTTGAATTGTTCATATGAGCGTAGCACAGTTATTTATTATTATTAGGCACTCTATTAGTCTATTGTCTTGCTAATACTAGTAAAGTAATATTTACTCTTCTGCTATGCTATTTCCTCAGCTTGGCGATGGTTTGGTGTATGAAATTGTGGACGGTTCAAATAGCTTCCCTCATTTTTATGGGCCTTCTCGAAGCTTTATCCCTCTCTCTCTAGATGTGGTCACAAAAGCGGAGAAGCTGTCTCTTTCAAATGGTCGGTTCAGTAGTAGTTTGCTTGATTAA

Protein sequence

>MS.gene050437.t1

MSDFVYRISTAKEWEELQRNGSTFGGELDKSSSFIHLSKLDQVRSTLDNFFLNSKDELYLLQIDAKKLGDGLVYEIVDGSNSFPHFYGPSRSFIPLSLDVVTKAEKLSLSNGRFSSSLLD