Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40285.t1 | RHN70880.1 | 95.7 | 93 | 1 | 1 | 1 | 90 | 44 | 136 | 9.80E-36 | 159.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40285.t1 | G7J3G6 | 95.7 | 93 | 1 | 1 | 1 | 90 | 1 | 93 | 7.1e-36 | 159.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene40285 | 0.800308 | 1.53E-48 | -1.69E-46 |
| MS.gene052664 | MS.gene40285 | 0.807299 | 5.41E-50 | -1.69E-46 |
| MS.gene052666 | MS.gene40285 | 0.804298 | 2.31E-49 | -1.69E-46 |
| MS.gene057641 | MS.gene40285 | 0.808454 | 3.07E-50 | -1.69E-46 |
| MS.gene057643 | MS.gene40285 | 0.809161 | 2.17E-50 | -1.69E-46 |
| MS.gene060432 | MS.gene40285 | 0.812437 | 4.24E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40285.t1 | MTR_3g109720 | 95.699 | 93 | 1 | 1 | 1 | 90 | 1 | 93 | 2.69e-56 | 168 |
| MS.gene40285.t1 | MTR_3g109720 | 89.412 | 85 | 6 | 1 | 1 | 82 | 1 | 85 | 8.11e-38 | 122 |
| MS.gene40285.t1 | MTR_4g100820 | 49.315 | 73 | 35 | 1 | 18 | 90 | 28 | 98 | 5.70e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40285.t1 | AT5G11630 | 60.215 | 93 | 34 | 1 | 1 | 90 | 1 | 93 | 5.82e-29 | 99.8 |
| MS.gene40285.t1 | AT5G11630 | 60.215 | 93 | 34 | 1 | 1 | 90 | 1 | 93 | 5.82e-29 | 99.8 |
| MS.gene40285.t1 | AT5G11630 | 54.118 | 85 | 36 | 1 | 1 | 82 | 1 | 85 | 2.64e-20 | 77.8 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT4G17310 | 66.000 | 50 | 17 | 0 | 41 | 90 | 50 | 99 | 1.80e-15 | 65.9 |
| MS.gene40285.t1 | AT5G47455 | 64.000 | 50 | 18 | 0 | 41 | 90 | 59 | 108 | 6.45e-15 | 64.7 |
| MS.gene40285.t1 | AT5G47455 | 64.000 | 50 | 18 | 0 | 41 | 90 | 59 | 108 | 6.45e-15 | 64.7 |
| MS.gene40285.t1 | AT5G47455 | 64.000 | 50 | 18 | 0 | 41 | 90 | 59 | 108 | 6.45e-15 | 64.7 |
| MS.gene40285.t1 | AT4G17310 | 73.171 | 41 | 11 | 0 | 41 | 81 | 50 | 90 | 6.99e-13 | 58.9 |
| MS.gene40285.t1 | AT4G17310 | 73.171 | 41 | 11 | 0 | 41 | 81 | 50 | 90 | 6.99e-13 | 58.9 |
| MS.gene40285.t1 | AT4G17310 | 73.171 | 41 | 11 | 0 | 41 | 81 | 50 | 90 | 6.99e-13 | 58.9 |
| MS.gene40285.t1 | AT4G17310 | 73.171 | 41 | 11 | 0 | 41 | 81 | 50 | 90 | 6.99e-13 | 58.9 |
| MS.gene40285.t1 | AT4G17310 | 73.171 | 41 | 11 | 0 | 41 | 81 | 50 | 90 | 6.99e-13 | 58.9 |
| MS.gene40285.t1 | AT5G47455 | 70.732 | 41 | 12 | 0 | 41 | 81 | 59 | 99 | 1.64e-12 | 58.2 |
| MS.gene40285.t1 | AT5G47455 | 70.732 | 41 | 12 | 0 | 41 | 81 | 59 | 99 | 1.64e-12 | 58.2 |
| MS.gene40285.t1 | AT5G47455 | 70.732 | 41 | 12 | 0 | 41 | 81 | 59 | 99 | 1.64e-12 | 58.2 |
| MS.gene40285.t1 | AT5G47455 | 70.732 | 41 | 12 | 0 | 41 | 81 | 59 | 99 | 1.64e-12 | 58.2 |
| MS.gene40285.t1 | AT5G47455 | 69.048 | 42 | 13 | 0 | 41 | 82 | 59 | 100 | 4.48e-12 | 57.4 |
| MS.gene40285.t1 | AT4G17310 | 69.048 | 42 | 13 | 0 | 41 | 82 | 50 | 91 | 7.30e-12 | 56.6 |
| MS.gene40285.t1 | AT4G17310 | 72.500 | 40 | 11 | 0 | 41 | 80 | 50 | 89 | 1.01e-11 | 56.2 |
| MS.gene40285.t1 | AT5G47455 | 70.000 | 40 | 12 | 0 | 41 | 80 | 59 | 98 | 1.63e-11 | 56.2 |
| MS.gene40285.t1 | AT5G47455 | 70.000 | 40 | 12 | 0 | 41 | 80 | 59 | 98 | 2.24e-11 | 55.5 |
| MS.gene40285.t1 | AT5G47455 | 70.000 | 40 | 12 | 0 | 41 | 80 | 59 | 98 | 2.24e-11 | 55.5 |
Find 25 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGGTGAGAAGGAGGATT+TGG | 0.218439 | 3.3:-90904334 | None:intergenic |
| AAGGGTGAGAAGGAGGATTT+GGG | 0.332675 | 3.3:-90904333 | None:intergenic |
| GGATTTGGGTTTGGTGATGG+TGG | 0.389511 | 3.3:-90904319 | None:intergenic |
| TGAGAAGGAGGATTTGGGTT+TGG | 0.407768 | 3.3:-90904328 | None:intergenic |
| AAGTTCGAGATCTTTGTCTC+AGG | 0.419533 | 3.3:+90904562 | MS.gene40285:CDS |
| TACAGGTCTGTCGCGGAGCT+TGG | 0.431386 | 3.3:+90904461 | MS.gene40285:intron |
| GAGGAGAAAGGGTGAGAAGG+AGG | 0.439652 | 3.3:-90904340 | None:intergenic |
| CTCGCACTTCCGTCACCGTC+AGG | 0.447447 | 3.3:+90904367 | MS.gene40285:CDS |
| GGAGGATTTGGGTTTGGTGA+TGG | 0.450114 | 3.3:-90904322 | None:intergenic |
| TTTGAGGAGAAAGGGTGAGA+AGG | 0.497764 | 3.3:-90904343 | None:intergenic |
| AGTAAGACGCGCTGATGAAA+CGG | 0.499949 | 3.3:-90904516 | None:intergenic |
| TGATGAAACGGCCGAGTGCA+AGG | 0.520941 | 3.3:-90904504 | None:intergenic |
| GCGCGTCTTACTTCTTGCCT+CGG | 0.528907 | 3.3:+90904527 | MS.gene40285:CDS |
| GTCACTCCTTCCCTTGCACT+CGG | 0.535547 | 3.3:+90904493 | MS.gene40285:CDS |
| AGTTCGAGATCTTTGTCTCA+GGG | 0.539010 | 3.3:+90904563 | MS.gene40285:CDS |
| GATTTGATTAGAGAGAATGC+TGG | 0.550233 | 3.3:-90904297 | None:intergenic |
| ATGCTTTGCAGTGCCAACCC+TGG | 0.558470 | 3.3:+90904587 | MS.gene40285:CDS |
| AATGAATCAAACACCAGGGT+TGG | 0.580607 | 3.3:-90904600 | None:intergenic |
| GATGAAACGGCCGAGTGCAA+GGG | 0.589191 | 3.3:-90904503 | None:intergenic |
| AGATAAATGAATCAAACACC+AGG | 0.590847 | 3.3:-90904605 | None:intergenic |
| AACTTGTTGAATCGATTCCG+AGG | 0.629649 | 3.3:-90904544 | None:intergenic |
| AAACGGCCGAGTGCAAGGGA+AGG | 0.634854 | 3.3:-90904499 | None:intergenic |
| GATGAGTTACCTGACGGTGA+CGG | 0.642581 | 3.3:-90904376 | None:intergenic |
| TCCACCGTACAGGTCTGTCG+CGG | 0.666992 | 3.3:+90904454 | MS.gene40285:intron |
| GATAAATGAATCAAACACCA+GGG | 0.682815 | 3.3:-90904604 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAATTAACGTTACATTAAAA+TGG | - | chr3.3:90904420-90904439 | None:intergenic | 20.0% |
| !! | TACATTAAAATGGAAGAAAA+AGG | - | chr3.3:90904410-90904429 | None:intergenic | 20.0% |
| ! | ATTAAAATGGAAGAAAAAGG+TGG | - | chr3.3:90904407-90904426 | None:intergenic | 25.0% |
| GATTTGATTAGAGAGAATGC+TGG | - | chr3.3:90904300-90904319 | None:intergenic | 35.0% | |
| AAGTTCGAGATCTTTGTCTC+AGG | + | chr3.3:90904562-90904581 | MS.gene40285:CDS | 40.0% | |
| AGTTCGAGATCTTTGTCTCA+GGG | + | chr3.3:90904563-90904582 | MS.gene40285:CDS | 40.0% | |
| ATGGAAGAAAAAGGTGGAAG+AGG | - | chr3.3:90904401-90904420 | None:intergenic | 40.0% | |
| !! | AACTTGTTGAATCGATTCCG+AGG | - | chr3.3:90904547-90904566 | None:intergenic | 40.0% |
| AAAGGGTGAGAAGGAGGATT+TGG | - | chr3.3:90904337-90904356 | None:intergenic | 45.0% | |
| AAGGGTGAGAAGGAGGATTT+GGG | - | chr3.3:90904336-90904355 | None:intergenic | 45.0% | |
| GAAGAGGATGAGTTACCTGA+CGG | - | chr3.3:90904385-90904404 | None:intergenic | 45.0% | |
| TTTGAGGAGAAAGGGTGAGA+AGG | - | chr3.3:90904346-90904365 | None:intergenic | 45.0% | |
| ! | AGTAAGACGCGCTGATGAAA+CGG | - | chr3.3:90904519-90904538 | None:intergenic | 45.0% |
| ! | TGAGAAGGAGGATTTGGGTT+TGG | - | chr3.3:90904331-90904350 | None:intergenic | 45.0% |
| !!! | AGTGCGAGTTTTGAGGAGAA+AGG | - | chr3.3:90904355-90904374 | None:intergenic | 45.0% |
| !!! | GTGCGAGTTTTGAGGAGAAA+GGG | - | chr3.3:90904354-90904373 | None:intergenic | 45.0% |
| GATGAGTTACCTGACGGTGA+CGG | - | chr3.3:90904379-90904398 | None:intergenic | 50.0% | |
| TCTTCTCTTCTCCACCGTAC+AGG | + | chr3.3:90904444-90904463 | MS.gene40285:intron | 50.0% | |
| !! | GGAGGATTTGGGTTTGGTGA+TGG | - | chr3.3:90904325-90904344 | None:intergenic | 50.0% |
| !! | GGATTTGGGTTTGGTGATGG+TGG | - | chr3.3:90904322-90904341 | None:intergenic | 50.0% |
| !! | TGACGGAAGTGCGAGTTTTG+AGG | - | chr3.3:90904362-90904381 | None:intergenic | 50.0% |
| ATGCTTTGCAGTGCCAACCC+TGG | + | chr3.3:90904587-90904606 | MS.gene40285:CDS | 55.0% | |
| GAGGAGAAAGGGTGAGAAGG+AGG | - | chr3.3:90904343-90904362 | None:intergenic | 55.0% | |
| GATGAAACGGCCGAGTGCAA+GGG | - | chr3.3:90904506-90904525 | None:intergenic | 55.0% | |
| GCGCGTCTTACTTCTTGCCT+CGG | + | chr3.3:90904527-90904546 | MS.gene40285:CDS | 55.0% | |
| GTCACTCCTTCCCTTGCACT+CGG | + | chr3.3:90904493-90904512 | MS.gene40285:CDS | 55.0% | |
| TGATGAAACGGCCGAGTGCA+AGG | - | chr3.3:90904507-90904526 | None:intergenic | 55.0% | |
| AAACGGCCGAGTGCAAGGGA+AGG | - | chr3.3:90904502-90904521 | None:intergenic | 60.0% | |
| AGCTCCGCGACAGACCTGTA+CGG | - | chr3.3:90904461-90904480 | None:intergenic | 60.0% | |
| TACAGGTCTGTCGCGGAGCT+TGG | + | chr3.3:90904461-90904480 | MS.gene40285:intron | 60.0% | |
| TCCACCGTACAGGTCTGTCG+CGG | + | chr3.3:90904454-90904473 | MS.gene40285:intron | 60.0% | |
| CTCGCACTTCCGTCACCGTC+AGG | + | chr3.3:90904367-90904386 | MS.gene40285:CDS | 65.0% | |
| TCCGCGACAGACCTGTACGG+TGG | - | chr3.3:90904458-90904477 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90904267 | 90904616 | 90904267 | ID=MS.gene40285 |
| chr3.3 | mRNA | 90904267 | 90904616 | 90904267 | ID=MS.gene40285.t1;Parent=MS.gene40285 |
| chr3.3 | exon | 90904267 | 90904388 | 90904267 | ID=MS.gene40285.t1.exon1;Parent=MS.gene40285.t1 |
| chr3.3 | CDS | 90904267 | 90904388 | 90904267 | ID=cds.MS.gene40285.t1;Parent=MS.gene40285.t1 |
| chr3.3 | exon | 90904466 | 90904616 | 90904466 | ID=MS.gene40285.t1.exon2;Parent=MS.gene40285.t1 |
| chr3.3 | CDS | 90904466 | 90904616 | 90904466 | ID=cds.MS.gene40285.t1;Parent=MS.gene40285.t1 |
| Gene Sequence |
| Protein sequence |
