AlfalfaGEDB | MS.gene060432

M. sativa cultivar XinJiangDaYe / MS.gene060432

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060432.t1	XP_004508161.1	98.3	60	1	0	1	60	1	60	2.80E-23	117.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060432.t1	Q9M7X7	85.0	60	9	0	1	60	1	60	1.9e-20	99.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060432.t1	I3SZB6	98.3	60	1	0	1	60	1	60	2.1e-23	117.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050134	MS.gene060432	0.810585	1.07E-50	-1.69E-46
MS.gene052664	MS.gene060432	0.841093	5.86E-58	-1.69E-46
MS.gene052666	MS.gene060432	0.818176	2.25E-52	-1.69E-46
MS.gene053224	MS.gene060432	0.803735	3.02E-49	-1.69E-46
MS.gene057237	MS.gene060432	0.818535	1.86E-52	-1.69E-46
MS.gene057641	MS.gene060432	0.868919	4.56E-66	-1.69E-46
MS.gene057643	MS.gene060432	0.852154	5.56E-61	-1.69E-46
MS.gene060432	MS.gene07244	0.898568	4.67E-77	-1.69E-46
MS.gene060432	MS.gene08870	0.807867	4.10E-50	-1.69E-46
MS.gene060432	MS.gene08871	0.820478	6.71E-53	-1.69E-46
MS.gene060432	MS.gene09038	0.815595	8.53E-52	-1.69E-46
MS.gene060432	MS.gene09204	0.817629	2.99E-52	-1.69E-46
MS.gene060432	MS.gene20766	0.837024	6.63E-57	-1.69E-46
MS.gene060432	MS.gene23031	0.855382	6.55E-62	-1.69E-46
MS.gene060432	MS.gene24639	0.823605	1.27E-53	-1.69E-46
MS.gene060432	MS.gene28147	0.850146	2.05E-60	-1.69E-46
MS.gene060432	MS.gene40285	0.812437	4.24E-51	-1.69E-46
MS.gene060432	MS.gene50756	0.83419	3.46E-56	-1.69E-46
MS.gene060432	MS.gene50758	0.851802	7.00E-61	-1.69E-46
MS.gene060432	MS.gene54912	0.839846	1.24E-57	-1.69E-46
MS.gene060432	MS.gene55619	0.824992	5.98E-54	-1.69E-46
MS.gene060432	MS.gene59230	0.840575	8.00E-58	-1.69E-46
MS.gene060432	MS.gene60693	0.827813	1.27E-54	-1.69E-46
MS.gene060432	MS.gene62561	0.933617	1.43E-95	-1.69E-46
MS.gene060432	MS.gene62695	0.84233	2.76E-58	-1.69E-46
MS.gene060432	MS.gene70316	0.811221	7.80E-51	-1.69E-46
MS.gene060432	MS.gene73833	0.819584	1.08E-52	-1.69E-46
MS.gene060432	MS.gene76031	0.808231	3.43E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene035828	MS.gene060432	PPI
MS.gene060432	MS.gene73093	PPI
MS.gene060432	MS.gene035825	PPI
MS.gene99872	MS.gene060432	PPI
MS.gene043645	MS.gene060432	PPI
MS.gene060432	MS.gene043595	PPI
MS.gene043593	MS.gene060432	PPI
MS.gene064353	MS.gene060432	PPI
MS.gene043643	MS.gene060432	PPI
MS.gene035826	MS.gene060432	PPI
MS.gene064351	MS.gene060432	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060432.t1	MTR_4g121877	98.333	60	1	0	1	60	1	60	7.50e-38	119
MS.gene060432.t1	MTR_4g121870	95.000	60	3	0	1	60	137	196	1.29e-36	120

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060432.t1	AT3G06680	85.000	60	9	1	60	23	82	2.96e-31	103
MS.gene060432.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene060432.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene060432.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene060432.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102
MS.gene060432.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102

Find 13 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGGCGGTGCCTCTT+TGG	0.285127	7.1:-66498425	None:intergenic
TATGCTTCCGTGCGTACCTC+TGG	0.442911	7.1:-66498579	None:intergenic
ACCGCCACACTTCCACCAAA+GGG	0.460737	7.1:+66498435	MS.gene060432:CDS
CGCTCACAACCAGTCTTACA+AGG	0.508995	7.1:+66498379	MS.gene060432:CDS
ATGATGAATACATACCCCTT+TGG	0.517971	7.1:-66498450	None:intergenic
CACCGCCACACTTCCACCAA+AGG	0.519273	7.1:+66498434	MS.gene060432:CDS
ATGAATACATACCCCTTTGG+TGG	0.547453	7.1:-66498447	None:intergenic
ATGGCATCAAGAAGCCAAAG+AGG	0.617235	7.1:+66498411	MS.gene060432:CDS
ATACCCCTTTGGTGGAAGTG+TGG	0.622392	7.1:-66498439	None:intergenic
CCCCTTTGGTGGAAGTGTGG+CGG	0.635065	7.1:-66498436	None:intergenic
CCGCCACACTTCCACCAAAG+GGG	0.674155	7.1:+66498436	MS.gene060432:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	7.1:-66498378	None:intergenic
TGAGGAACCAGAGGTACGCA+CGG	0.675026	7.1:+66498572	MS.gene060432:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGTGATTGATTACTGTAGA+TGG	+	chr7.1:66498537-66498556	MS.gene060432:intron	30.0%
	ATGATGAATACATACCCCTT+TGG	-	chr7.1:66498453-66498472	None:intergenic	35.0%
!	AGATGGATCCAAAGTTTTTG+AGG	+	chr7.1:66498554-66498573	MS.gene060432:intron	35.0%
!	CGGAAGCATAACAAGAAAAA+TGG	+	chr7.1:66498592-66498611	MS.gene060432:CDS	35.0%
!!!	CTTCTTGATGCCATTTTTGT+GGG	-	chr7.1:66498405-66498424	None:intergenic	35.0%
	ATGAATACATACCCCTTTGG+TGG	-	chr7.1:66498450-66498469	None:intergenic	40.0%
	TCTTACAAGGCCCACAAAAA+TGG	+	chr7.1:66498392-66498411	MS.gene060432:CDS	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	-	chr7.1:66498565-66498584	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	+	chr7.1:66498563-66498582	MS.gene060432:CDS	40.0%
!!!	GCTTCTTGATGCCATTTTTG+TGG	-	chr7.1:66498406-66498425	None:intergenic	40.0%
	ATGGCATCAAGAAGCCAAAG+AGG	+	chr7.1:66498411-66498430	MS.gene060432:CDS	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	-	chr7.1:66498381-66498400	None:intergenic	45.0%
!!	TTTTGTGGGCCTTGTAAGAC+TGG	-	chr7.1:66498391-66498410	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	-	chr7.1:66498442-66498461	None:intergenic	50.0%
	CGCTCACAACCAGTCTTACA+AGG	+	chr7.1:66498379-66498398	MS.gene060432:CDS	50.0%
	ACCGCCACACTTCCACCAAA+GGG	+	chr7.1:66498435-66498454	MS.gene060432:CDS	55.0%
	TATGCTTCCGTGCGTACCTC+TGG	-	chr7.1:66498582-66498601	None:intergenic	55.0%
!	TGAGGAACCAGAGGTACGCA+CGG	+	chr7.1:66498572-66498591	MS.gene060432:CDS	55.0%
	CACCGCCACACTTCCACCAA+AGG	+	chr7.1:66498434-66498453	MS.gene060432:CDS	60.0%
	CCGCCACACTTCCACCAAAG+GGG	+	chr7.1:66498436-66498455	MS.gene060432:CDS	60.0%
	GAAGTGTGGCGGTGCCTCTT+TGG	-	chr7.1:66498428-66498447	None:intergenic	60.0%
!	CCCCTTTGGTGGAAGTGTGG+CGG	-	chr7.1:66498439-66498458	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	66498356	66498636	66498356	ID=MS.gene060432
chr7.1	mRNA	66498356	66498636	66498356	ID=MS.gene060432.t1;Parent=MS.gene060432
chr7.1	exon	66498356	66498457	66498356	ID=MS.gene060432.t1.exon1;Parent=MS.gene060432.t1
chr7.1	CDS	66498356	66498457	66498356	ID=cds.MS.gene060432.t1;Parent=MS.gene060432.t1
chr7.1	exon	66498556	66498636	66498556	ID=MS.gene060432.t1.exon2;Parent=MS.gene060432.t1
chr7.1	CDS	66498556	66498636	66498556	ID=cds.MS.gene060432.t1;Parent=MS.gene060432.t1

Gene Sequence	>MS.gene060432 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCCCACAAAAATGGCATCAAGAAGCCAAAGAGGCACCGCCACACTTCCACCAAAGGGGTATGTATTCATCATTCTTCTCTGATTTTTCAAACGATTGTTCTATTTTGTAATGAGATTTTAATTAATTGTTTATTTATTGTGATTGATTACTGTAGATGGATCCAAAGTTTTTGAGGAACCAGAGGTACGCACGGAAGCATAACAAGAAAAATGGTGAAATTGCAGAAGAAGAGTAA
Protein sequence	>MS.gene060432.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIAEEE