AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40442

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40442.t1 MTR_6g477820 97.802 728 14 2 1 726 1 728 0.0 1487
MS.gene40442.t1 MTR_7g427710 64.246 716 172 12 17 724 2 641 0.0 878
MS.gene40442.t1 MTR_8g432540 48.452 743 344 10 2 725 88 810 0.0 681
MS.gene40442.t1 MTR_7g076080 49.721 718 322 12 23 725 85 778 0.0 679
MS.gene40442.t1 MTR_4g047800 47.476 733 346 14 2 726 33 734 0.0 664
MS.gene40442.t1 MTR_5g029320 48.942 709 336 15 29 726 89 782 0.0 661
MS.gene40442.t1 MTR_2g088470 48.077 728 338 14 2 722 39 733 0.0 655
MS.gene40442.t1 MTR_1g101280 47.472 712 342 12 27 721 54 750 0.0 640
MS.gene40442.t1 MTR_7g427690 71.465 389 91 5 203 585 38 412 0.0 566
MS.gene40442.t1 MTR_2g101720 41.026 702 350 12 33 723 143 791 0.0 564
MS.gene40442.t1 MTR_0198s0030 42.458 716 362 15 33 721 59 751 0.0 534
MS.gene40442.t1 MTR_2g030570 40.928 733 383 17 1 721 1 695 2.97e-172 510
MS.gene40442.t1 MTR_2g030600 40.629 731 381 18 1 721 1 688 1.51e-165 493
MS.gene40442.t1 MTR_5g005600 37.360 712 373 17 36 721 52 716 2.24e-150 455
MS.gene40442.t1 MTR_2g030660 34.930 710 384 19 21 721 7 647 2.30e-131 404
MS.gene40442.t1 MTR_7g427700 58.912 331 75 4 17 339 2 279 2.29e-120 367
MS.gene40442.t1 MTR_7g427700 64.072 167 47 4 558 724 281 434 6.69e-52 186
MS.gene40442.t1 MTR_0653s0020 66.837 196 52 3 403 598 1 183 1.17e-82 261
MS.gene40442.t1 MTR_2g083330 35.033 451 257 11 280 722 104 526 6.63e-77 257
MS.gene40442.t1 MTR_2g083350 32.963 540 287 19 228 722 23 532 2.35e-76 256
MS.gene40442.t1 MTR_5g046040 63.473 167 60 1 27 192 31 197 1.07e-67 228
MS.gene40442.t1 MTR_7g027265 68.657 134 39 1 18 148 3 136 1.60e-53 181
MS.gene40442.t1 MTR_7g027605 76.087 92 21 1 309 400 1 91 3.91e-44 154
MS.gene40442.t1 MTR_2g030690 32.013 303 172 9 427 721 1 277 9.53e-39 145
MS.gene40442.t1 MTR_0653s0010 63.077 130 35 4 595 724 1 117 3.60e-37 135
MS.gene40442.t1 MTR_2g451350 43.284 134 74 2 234 366 1 133 2.14e-28 111
MS.gene40442.t1 MTR_7g427720 69.412 85 17 3 640 724 23 98 1.86e-24 98.6
MS.gene40442.t1 MTR_7g427670 59.770 87 24 1 83 169 32 107 2.87e-24 99.0
MS.gene40442.t1 MTR_6g027290 33.728 169 105 3 1 167 1 164 7.33e-23 96.7
MS.gene40442.t1 MTR_6g086740 87.755 49 6 0 678 726 4 52 3.49e-20 84.7
MS.gene40442.t1 MTR_7g027245 57.143 77 25 1 229 305 19 87 6.61e-20 85.9
MS.gene40442.t1 MTR_1g036590 86.486 37 5 0 340 376 238 274 1.36e-13 73.9
MS.gene40442.t1 MTR_2g030720 30.833 120 71 2 33 152 22 129 5.94e-13 67.8
MS.gene40442.t1 MTR_4g058970 30.769 182 90 6 18 163 9 190 1.18e-12 72.0
MS.gene40442.t1 MTR_1g105420 29.381 194 104 6 84 267 1 171 2.98e-12 67.0
MS.gene40442.t1 MTR_7g103340 38.835 103 60 1 25 124 56 158 4.66e-12 67.0
MS.gene40442.t1 MTR_2g451350 46.667 60 32 0 307 366 35 94 8.64e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40442.t1 AT1G73360 63.764 712 227 13 25 726 28 718 0.0 891
MS.gene40442.t1 AT1G17920 59.147 727 240 15 12 726 5 686 0.0 838
MS.gene40442.t1 AT1G17920 59.147 727 240 15 12 726 5 686 0.0 838
MS.gene40442.t1 AT4G04890 51.836 708 304 16 26 723 57 737 0.0 699
MS.gene40442.t1 AT4G04890 51.836 708 304 16 26 723 57 737 0.0 699
MS.gene40442.t1 AT4G21750 50.419 716 316 15 29 723 62 759 0.0 686
MS.gene40442.t1 AT4G21750 50.419 716 316 15 29 723 62 759 0.0 686
MS.gene40442.t1 AT4G21750 50.419 716 316 15 29 723 62 759 0.0 686
MS.gene40442.t1 AT4G21750 50.419 716 316 15 29 723 62 759 0.0 686
MS.gene40442.t1 AT1G05230 47.665 728 323 12 10 726 39 719 0.0 672
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT1G05230 47.534 730 323 13 10 726 39 721 0.0 667
MS.gene40442.t1 AT4G00730 47.139 734 340 12 2 725 107 802 0.0 641
MS.gene40442.t1 AT3G61150 46.732 719 344 15 28 726 109 808 0.0 633
MS.gene40442.t1 AT5G46880 44.626 735 344 19 20 721 102 806 0.0 572
MS.gene40442.t1 AT5G46880 44.626 735 344 19 20 721 102 806 0.0 572
MS.gene40442.t1 AT5G46880 44.626 735 344 19 20 721 102 806 0.0 572
MS.gene40442.t1 AT1G05230 49.556 563 254 10 29 586 64 601 0.0 549
MS.gene40442.t1 AT5G52170 43.040 704 308 15 33 725 61 682 0.0 537
MS.gene40442.t1 AT5G52170 43.040 704 308 15 33 725 61 682 0.0 537
MS.gene40442.t1 AT5G52170 42.716 707 311 15 33 725 61 687 0.0 534
MS.gene40442.t1 AT3G03260 40.921 738 367 17 9 724 7 697 3.43e-180 531
MS.gene40442.t1 AT1G79840 39.093 706 357 18 27 721 41 684 2.98e-172 510
MS.gene40442.t1 AT1G79840 39.093 706 357 18 27 721 41 684 2.98e-172 510
MS.gene40442.t1 AT1G79840 39.093 706 357 18 27 721 99 742 1.42e-171 510
MS.gene40442.t1 AT1G79840 39.093 706 357 18 27 721 128 771 8.99e-171 509
MS.gene40442.t1 AT2G32370 39.261 731 375 18 12 726 48 725 3.55e-164 491
MS.gene40442.t1 AT5G17320 40.434 737 368 18 21 723 18 717 1.13e-162 486
MS.gene40442.t1 AT4G17710 39.660 706 323 18 29 721 88 703 3.82e-159 477
MS.gene40442.t1 AT1G34650 40.247 728 377 17 21 723 13 707 1.07e-154 466
MS.gene40442.t1 AT1G79840 40.767 574 281 17 27 588 99 625 2.53e-143 434
MS.gene40442.t1 AT4G00730 48.608 467 211 9 2 461 107 551 1.37e-139 422
MS.gene40442.t1 AT3G03260 41.697 554 295 12 9 556 7 538 2.61e-137 415
MS.gene40442.t1 AT4G25530 34.807 701 393 16 29 722 40 683 8.96e-128 395
MS.gene40442.t1 AT1G79840 41.138 457 212 14 27 472 99 509 3.72e-105 331
MS.gene40442.t1 AT5G07260 24.910 554 333 19 96 641 11 489 1.25e-39 154
MS.gene40442.t1 AT5G07260 24.729 554 329 20 96 641 11 484 3.24e-39 153
MS.gene40442.t1 AT4G26920 22.976 457 252 15 201 649 54 418 5.70e-19 90.9
MS.gene40442.t1 AT5G60690 37.121 132 56 5 18 122 7 138 6.25e-14 76.3
MS.gene40442.t1 AT1G30490 34.821 112 65 2 15 118 4 115 2.23e-11 67.8
MS.gene40442.t1 AT1G52150 20.961 458 301 17 313 722 228 672 7.73e-11 66.2
MS.gene40442.t1 AT1G52150 20.961 458 301 17 313 722 228 672 7.85e-11 66.2
MS.gene40442.t1 AT1G52150 20.742 458 303 16 313 722 228 673 8.20e-11 66.2

Find 156 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTGGTTGAAATGTTTA+TGG 0.134213 6.2:+75982592 MS.gene40442:CDS
GACTTATCAATGGGAAATTT+TGG 0.144806 6.2:+75982239 MS.gene40442:CDS
CGCAAATTGAGCAAGGTTTA+TGG 0.148416 6.2:+75983038 MS.gene40442:CDS
AACAGTTGCATTCCAAATTA+TGG 0.179679 6.2:+75984913 MS.gene40442:CDS
CTCCTAGACAGATCAAATTT+TGG 0.209657 6.2:+75980936 MS.gene40442:CDS
GTAAACTACTAACCATAATT+TGG 0.226737 6.2:-75984925 None:intergenic
ACTATTGAAGTTATCTCTTC+TGG 0.240170 6.2:+75982765 MS.gene40442:CDS
GAGTCTGTTGCAACTGTTAA+TGG 0.255130 6.2:+75984968 MS.gene40442:CDS
TTCAACCGATTATTAGTCTT+AGG 0.265573 6.2:-75982509 None:intergenic
GCAACTGTTAATGGACTTAT+TGG 0.266967 6.2:+75984977 MS.gene40442:CDS
TAGTGGGATGTTCTTTCTAA+TGG 0.297445 6.2:+75984151 MS.gene40442:intron
TTCATTGATGTTAATTCAAT+TGG 0.303666 6.2:-75984707 None:intergenic
ATTTCCTACTATTGTGACTA+TGG 0.304117 6.2:+75982737 MS.gene40442:CDS
GGTCACTTGGATTGAACATT+TGG 0.308899 6.2:+75983260 MS.gene40442:intron
TGAAAGGCTTGCTTGTTCTA+TGG 0.312367 6.2:+75983386 MS.gene40442:CDS
TGCATAGTTGATGAACTTCC+AGG 0.324010 6.2:-75982308 None:intergenic
ATCTATAGTGGAGCTGCATT+TGG 0.334195 6.2:+75983327 MS.gene40442:CDS
CACATTCCAGTAACACCTTC+AGG 0.343396 6.2:+75984758 MS.gene40442:CDS
GCAACTCCATCTCTGTGCTT+AGG 0.352073 6.2:+75984221 MS.gene40442:CDS
GATTGGTCTTCCAATGTATT+TGG 0.360707 6.2:-75982174 None:intergenic
ATGTGAGAATATAGCTATAA+GGG 0.368026 6.2:+75981485 MS.gene40442:CDS
GTGAAACCTGAAGGTGTTAC+TGG 0.378283 6.2:-75984764 None:intergenic
GCAAATTGAGCAAGGTTTAT+GGG 0.381720 6.2:+75983039 MS.gene40442:CDS
TTCATCATTGGACTTATCAA+TGG 0.382138 6.2:+75982229 MS.gene40442:CDS
ATCGAAATTGAGGAGCAAAT+TGG 0.385407 6.2:-75983099 None:intergenic
AAGAAATTGAAAACAGTTTG+TGG 0.386401 6.2:-75984012 None:intergenic
TGCAACCTTTGTTTCTCATC+AGG 0.387813 6.2:-75980890 None:intergenic
GAAGCTTCAATGCGAATATT+CGG 0.387981 6.2:-75982536 None:intergenic
ACCAATTCACATTTCATCAT+TGG 0.388149 6.2:+75982217 MS.gene40442:CDS
CGATGCCATCGTCTTCCATC+TGG 0.388366 6.2:+75983118 MS.gene40442:CDS
TCTCGTGTTGTTATTATGAA+TGG 0.397911 6.2:+75982566 MS.gene40442:CDS
GAGAATCAGAAGAACCTTCA+TGG 0.402346 6.2:-75980636 None:intergenic
GATGTGAGAATATAGCTATA+AGG 0.404730 6.2:+75981484 MS.gene40442:CDS
CAACTCCATCTCTGTGCTTA+GGG 0.406270 6.2:+75984222 MS.gene40442:CDS
TTTGGTGGGCATGGAATGGT+TGG 0.409505 6.2:+75982257 MS.gene40442:CDS
ATTACCTTGGAGTAGCCATT+AGG 0.412515 6.2:-75983160 None:intergenic
TCCTAGTGGTTCTATGCAAC+TGG 0.413430 6.2:+75982800 MS.gene40442:CDS
TATAGAAATGCTATCTATAG+TGG 0.415183 6.2:+75983315 MS.gene40442:CDS
GATAACAGTAATAATCTTCA+AGG 0.418756 6.2:+75984812 MS.gene40442:CDS
TTTCTATAAAGCCTATGAAC+TGG 0.420383 6.2:-75983300 None:intergenic
GGTGGGCATGGAATGGTTGG+AGG 0.429152 6.2:+75982260 MS.gene40442:CDS
CTTGATCTTGATCTTCTACC+TGG 0.430302 6.2:+75982290 MS.gene40442:CDS
GTGCATCAGCTAGTCATAGA+TGG 0.431673 6.2:+75983872 MS.gene40442:CDS
CTGCTCTTTCCCTCAGGTGA+TGG 0.432769 6.2:-75983799 None:intergenic
AAGAAACAATCATCATTGAT+TGG 0.440524 6.2:-75981549 None:intergenic
TATTATGAATGGCTTAGCTT+TGG 0.440677 6.2:+75982577 MS.gene40442:CDS
GAAGTTGCTCATATTGCAAA+TGG 0.443252 6.2:+75984187 MS.gene40442:CDS
AAAACAGTTTGTGGAGGGAG+AGG 0.444588 6.2:-75984003 None:intergenic
AGGGAGTCAGAGTTACTGTT+AGG 0.446651 6.2:+75983917 MS.gene40442:CDS
GGTTTCACAATCACTTCTGA+TGG 0.448901 6.2:+75984779 MS.gene40442:CDS
AGTTATCTCTTCTGGAATGA+TGG 0.450155 6.2:+75982773 MS.gene40442:CDS
ATCATGCTGCTCTTTCCCTC+AGG 0.452513 6.2:-75983805 None:intergenic
TCCAATGATGAAATGTGAAT+TGG 0.452865 6.2:-75982218 None:intergenic
CTTGCCATAGTCACAATAGT+AGG 0.453943 6.2:-75982741 None:intergenic
TCAACCGATTATTAGTCTTA+GGG 0.457224 6.2:-75982508 None:intergenic
ATGCAATGGAAGAGATGATT+AGG 0.457268 6.2:+75982399 MS.gene40442:CDS
GAAAGACAAGCTGGTTGGTA+AGG 0.465607 6.2:-75982338 None:intergenic
ACTGGTGGAAGCTGTGTGAT+TGG 0.466182 6.2:-75982191 None:intergenic
CACACTTTCAGCTTTGAATG+AGG 0.466242 6.2:+75983897 MS.gene40442:CDS
AAGAAGTAGAATTCTCTGAT+AGG 0.466986 6.2:-75983004 None:intergenic
TTCATTCAAGACATGCCTAA+TGG 0.467119 6.2:+75983145 MS.gene40442:CDS
TCATCATTGGACTTATCAAT+GGG 0.471502 6.2:+75982230 MS.gene40442:CDS
AGTAATAATCTTCAAGGAAG+TGG 0.472661 6.2:+75984818 MS.gene40442:CDS
CACTTCCACCATTGTTACCT+AGG 0.476114 6.2:-75984877 None:intergenic
GATTATTACTGTTATCAAGT+TGG 0.486920 6.2:-75984805 None:intergenic
GTTAAGCAGAGAATTAGCTT+TGG 0.491594 6.2:+75980913 MS.gene40442:CDS
GAAAGCTGTGTGGATTCATC+AGG 0.491631 6.2:+75984665 MS.gene40442:CDS
CTAGGTAACAATGGTGGAAG+TGG 0.492689 6.2:+75984878 MS.gene40442:CDS
GTTTCAGCCACCCGTGATCT+CGG 0.495016 6.2:+75983408 MS.gene40442:CDS
TATGTGCAGCTACTACCATT+TGG 0.503380 6.2:+75983977 MS.gene40442:CDS
CTTTGTTTCTCATCAGGATG+AGG 0.506519 6.2:-75980884 None:intergenic
TCAATTTCTTCAAAGATGAA+AGG 0.508178 6.2:+75984025 MS.gene40442:CDS
CTGCATTTGGAGCTGAAAGA+TGG 0.510050 6.2:+75983340 MS.gene40442:CDS
GAAATGTGAATTGGTGCAAC+TGG 0.511399 6.2:-75982209 None:intergenic
AGTGGAGCAAGTGGGTCCCC+TGG 0.514639 6.2:+75980592 MS.gene40442:CDS
TGTTCCCTAAGACTAATAAT+CGG 0.516268 6.2:+75982504 MS.gene40442:CDS
AGACGATGGCATCGAAATTG+AGG 0.521276 6.2:-75983109 None:intergenic
AGCACAGAGATGGAGTTGCC+AGG 0.521625 6.2:-75984217 None:intergenic
TTAATCTTGAAAACTATGAA+AGG 0.523531 6.2:+75982480 MS.gene40442:CDS
GCCACCCGTGATCTCGGTGG+AGG 0.523884 6.2:+75983414 MS.gene40442:CDS
TATTCGCATTGAAGCTTCGA+GGG 0.530981 6.2:+75982541 MS.gene40442:CDS
TCCATGTCAGAAAGACAAGC+TGG 0.531398 6.2:-75982347 None:intergenic
CTCTTCAGAGAATGTGTGAA+AGG 0.534959 6.2:+75983370 MS.gene40442:CDS
GAGCTGATAACTGTCAACTA+AGG 0.535625 6.2:+75981442 MS.gene40442:CDS
GTTATCTCTTCTGGAATGAT+GGG 0.546482 6.2:+75982774 MS.gene40442:CDS
GAACAGAAGGACTCAGATGA+AGG 0.553957 6.2:+75980964 MS.gene40442:CDS
AGCATTGCTGCCAAATACAT+TGG 0.554442 6.2:+75982164 MS.gene40442:CDS
ATATACCTCCACCGAGATCA+CGG 0.554654 6.2:-75983419 None:intergenic
GTTGTCATGAGGAAAATCAT+AGG 0.556836 6.2:-75983077 None:intergenic
ACACTTTCAGCTTTGAATGA+GGG 0.558221 6.2:+75983898 MS.gene40442:CDS
AATTGAAAACAGTTTGTGGA+GGG 0.561046 6.2:-75984008 None:intergenic
TACTGTACGCAAATTGAGCA+AGG 0.562069 6.2:+75983031 MS.gene40442:CDS
GCACAGAGATGGAGTTGCCA+GGG 0.562213 6.2:-75984216 None:intergenic
AGTGGTAATGTTAATGCTGA+TGG 0.563501 6.2:+75984836 MS.gene40442:CDS
ATCTCTTCTGGAATGATGGG+TGG 0.566526 6.2:+75982777 MS.gene40442:CDS
GTGATCTGCCCCTCTTGTGG+TGG 0.567379 6.2:+75981522 MS.gene40442:CDS
ACCAGCTTGTCTTTCTGACA+TGG 0.569306 6.2:+75982346 MS.gene40442:CDS
ACCATCTGCTAAACTTAACA+TGG 0.570228 6.2:+75984946 MS.gene40442:CDS
AGATGAAAGGAAAAGATCAC+AGG 0.570557 6.2:+75984038 MS.gene40442:CDS
CATCATCATCATCACCATGA+AGG 0.573382 6.2:+75980622 MS.gene40442:CDS
GCACATAGCACAACACCACT+AGG 0.575221 6.2:-75983961 None:intergenic
GCATCAACAAGCTCAAACCT+AGG 0.578527 6.2:+75984860 MS.gene40442:CDS
TCAGCGATTCCATCACCTGA+GGG 0.581584 6.2:+75983790 MS.gene40442:intron
AAATGTGCAACTCAAAGAAG+AGG 0.585426 6.2:+75981596 MS.gene40442:CDS
AACCAAAATTTGATCTGTCT+AGG 0.585787 6.2:-75980938 None:intergenic
TCTTGAATGAAGCAGCCAGA+TGG 0.586658 6.2:-75983133 None:intergenic
CTGAAGGTGTTACTGGAATG+TGG 0.586831 6.2:-75984757 None:intergenic
ATTGCAAATGGTTCTCACCC+TGG 0.587680 6.2:+75984199 MS.gene40442:CDS
GGTAACAATGGTGGAAGTGG+AGG 0.589507 6.2:+75984881 MS.gene40442:CDS
TCTTACCCTAAGCACAGAGA+TGG 0.590044 6.2:-75984227 None:intergenic
TCCATGTTAAGTTTAGCAGA+TGG 0.590444 6.2:-75984947 None:intergenic
CTCATTCAAAGCTGAAAGTG+TGG 0.591958 6.2:-75983896 None:intergenic
ATATTCGCATTGAAGCTTCG+AGG 0.592812 6.2:+75982540 MS.gene40442:CDS
TGGAGGGAGAGGTAGCCAAA+TGG 0.596344 6.2:-75983992 None:intergenic
CTACTATTGTGACTATGGCA+AGG 0.599386 6.2:+75982742 MS.gene40442:CDS
CTCATCCTGATGAGAAACAA+AGG 0.606148 6.2:+75980885 MS.gene40442:CDS
GATTGGAGGACCACCACAAG+AGG 0.608794 6.2:-75981532 None:intergenic
AAACAATCATCATTGATTGG+AGG 0.609512 6.2:-75981546 None:intergenic
TATACCTCCACCGAGATCAC+GGG 0.611974 6.2:-75983418 None:intergenic
GAGTATCATAGTGGAGCAAG+TGG 0.613997 6.2:+75980583 MS.gene40442:CDS
TGGATGAAATCAAACTCTGA+TGG 0.615224 6.2:+75982446 MS.gene40442:CDS
ACACTGCTAATCAGATTCAA+AGG 0.615484 6.2:+75980686 MS.gene40442:CDS
GATGAAGCTAGCACAAAGGA+TGG 0.618354 6.2:+75983825 MS.gene40442:CDS
TTCAGCGATTCCATCACCTG+AGG 0.619756 6.2:+75983789 MS.gene40442:intron
GTTAGGAAGAGTACTGAACT+TGG 0.620703 6.2:+75983934 MS.gene40442:CDS
TAGGAAATAGAGCCATCCAC+TGG 0.622461 6.2:-75982722 None:intergenic
AGCTCAAACCTAGGTAACAA+TGG 0.624081 6.2:+75984869 MS.gene40442:CDS
TGCAGACTAATGAGCCTCTG+TGG 0.627832 6.2:+75982426 MS.gene40442:CDS
GGAATGATGGGTGGTCCTAG+TGG 0.629709 6.2:+75982786 MS.gene40442:CDS
AAATTGAAAACAGTTTGTGG+AGG 0.630363 6.2:-75984009 None:intergenic
TGACATTGCATCGAATGCAA+TGG 0.631078 6.2:+75982385 MS.gene40442:CDS
AGAAGATCAAGATCAAGTGA+AGG 0.633385 6.2:-75982284 None:intergenic
AATTCTCTGATAGGAACAAG+TGG 0.634478 6.2:-75982995 None:intergenic
TGTCAGAAAGACAAGCTGGT+TGG 0.640801 6.2:-75982343 None:intergenic
ATGTGAATTGGTGCAACTGG+TGG 0.640938 6.2:-75982206 None:intergenic
GGAGCAAATTGGTTGTCATG+AGG 0.644218 6.2:-75983088 None:intergenic
ACCTCCACCGAGATCACGGG+TGG 0.644662 6.2:-75983415 None:intergenic
AATGTGATCTGCCCCTCTTG+TGG 0.645075 6.2:+75981519 MS.gene40442:CDS
ATTGGAGGACCACCACAAGA+GGG 0.645788 6.2:-75981531 None:intergenic
CATGCCTAATGGCTACTCCA+AGG 0.648553 6.2:+75983156 MS.gene40442:CDS
ACTGAACTTGGTCAGCCTAG+TGG 0.649999 6.2:+75983946 MS.gene40442:CDS
GAAGTGATTGTGAAACCTGA+AGG 0.654254 6.2:-75984773 None:intergenic
GAATCTGATTAGCAGTGTGA+CGG 0.657531 6.2:-75980681 None:intergenic
TCAGCCACCCGTGATCTCGG+TGG 0.670096 6.2:+75983411 MS.gene40442:CDS
TGAAGGTGTTACTGGAATGT+GGG 0.675743 6.2:-75984756 None:intergenic
GCATGATGAAGCTAGCACAA+AGG 0.680662 6.2:+75983821 MS.gene40442:CDS
GAGTTTGATTTCATCCACAG+AGG 0.690229 6.2:-75982440 None:intergenic
TTGGAGGACCACCACAAGAG+GGG 0.698087 6.2:-75981530 None:intergenic
AGCAGCCAGATGGAAGACGA+TGG 0.710624 6.2:-75983123 None:intergenic
TCAAACCTAGGTAACAATGG+TGG 0.711311 6.2:+75984872 MS.gene40442:CDS
AGTATCATAGTGGAGCAAGT+GGG 0.711511 6.2:+75980584 MS.gene40442:CDS
TCTGATTAGCAGTGTGACGG+TGG 0.712245 6.2:-75980678 None:intergenic
TCAATGAATGTAGCAATGAG+TGG 0.714910 6.2:+75984722 MS.gene40442:CDS
ACCAGTTGCATAGAACCACT+AGG 0.723246 6.2:-75982801 None:intergenic
GATACTTCAAGAAAGCTGTG+TGG 0.771950 6.2:+75984655 MS.gene40442:CDS
ATGATGATGATGATCACCAG+GGG 0.774959 6.2:-75980608 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAAAATATAAAAAAA+TGG - chr6.2:75980820-75980839 None:intergenic 0.0%
!! GAAATAAAAAATATAAAACA+TGG - chr6.2:75982073-75982092 None:intergenic 10.0%
!! GAAGAAAAAAAATAAAAAAT+AGG - chr6.2:75980846-75980865 None:intergenic 10.0%
!!! ATATTTATATTTAACTTTCT+TGG + chr6.2:75983733-75983752 MS.gene40442:intron 10.0%
!!! TTTAAAAATTGTGATTTTAT+AGG - chr6.2:75981716-75981735 None:intergenic 10.0%
!! ACAAAATAATGAAAATGAAA+AGG - chr6.2:75982642-75982661 None:intergenic 15.0%
!! TACATTGATAAATATGTATA+TGG - chr6.2:75983701-75983720 None:intergenic 15.0%
!!! ATTTACTATGTACTTTTATA+TGG + chr6.2:75982094-75982113 MS.gene40442:intron 15.0%
!!! ATTTATGTGTTATTTTACAA+TGG + chr6.2:75981379-75981398 MS.gene40442:intron 15.0%
!!! TGTATTTTTTTATTATTTCC+TGG + chr6.2:75981124-75981143 MS.gene40442:intron 15.0%
!!! TGTGTATTTTATATTTATGT+TGG + chr6.2:75981159-75981178 MS.gene40442:intron 15.0%
!!! TTAATAAAGTATTGATTCTA+AGG - chr6.2:75983190-75983209 None:intergenic 15.0%
!!! TTTTATATGGTTAATTGAAA+TGG + chr6.2:75982107-75982126 MS.gene40442:intron 15.0%
!! ATAATTGCACAAACATTTAT+AGG + chr6.2:75981781-75981800 MS.gene40442:intron 20.0%
!! ATTATAAAGTTTAGGTGAAT+TGG + chr6.2:75981295-75981314 MS.gene40442:intron 20.0%
!! ATTCAATGTTGAATAGAAAT+CGG - chr6.2:75982910-75982929 None:intergenic 20.0%
!! GGATGAAAAAATGAATAAAA+TGG - chr6.2:75984263-75984282 None:intergenic 20.0%
!! TAATTGCACAAACATTTATA+GGG + chr6.2:75981782-75981801 MS.gene40442:intron 20.0%
!! TACACATTAATTAATAAACC+AGG - chr6.2:75981145-75981164 None:intergenic 20.0%
!! TGAAAAAATGAATAAAATGG+AGG - chr6.2:75984260-75984279 None:intergenic 20.0%
!! TTAATCTTGAAAACTATGAA+AGG + chr6.2:75982480-75982499 MS.gene40442:CDS 20.0%
!! TTATAAAGTTTAGGTGAATT+GGG + chr6.2:75981296-75981315 MS.gene40442:intron 20.0%
!! TTCATTGATGTTAATTCAAT+TGG - chr6.2:75984710-75984729 None:intergenic 20.0%
!!! AAAAAGATCGTGATTTTTAT+AGG + chr6.2:75981815-75981834 MS.gene40442:intron 20.0%
!!! AAATTGCGTATTTTTTTTTC+TGG + chr6.2:75981079-75981098 MS.gene40442:intron 20.0%
!!! AGAACAAAATTTTCATTCTA+AGG - chr6.2:75984103-75984122 None:intergenic 20.0%
!!! ATAAAGTTTGTGTATTTTTC+TGG + chr6.2:75981201-75981220 MS.gene40442:intron 20.0%
!!! CACTAAAAATATTTTGACAT+CGG + chr6.2:75982834-75982853 MS.gene40442:intron 20.0%
!!! GTTACTCTTGTATATTTTAT+AGG + chr6.2:75983239-75983258 MS.gene40442:intron 20.0%
!!! TGTGAATTTTCAAATGTAAT+AGG + chr6.2:75984604-75984623 MS.gene40442:intron 20.0%
!!! TTGAAAATTTTAGATGTATG+AGG + chr6.2:75982955-75982974 MS.gene40442:intron 20.0%
!!! TTTCTTTGTTTTTGTTTAGT+GGG + chr6.2:75984135-75984154 MS.gene40442:intron 20.0%
! AACATGTTGCAACTAAAAAA+TGG - chr6.2:75984511-75984530 None:intergenic 25.0%
! AAGAAACAATCATCATTGAT+TGG - chr6.2:75981552-75981571 None:intergenic 25.0%
! AAGAAATTGAAAACAGTTTG+TGG - chr6.2:75984015-75984034 None:intergenic 25.0%
! AATGAAAATGAAAAGGAACA+AGG - chr6.2:75982635-75982654 None:intergenic 25.0%
! ACATGTTGCAACTAAAAAAT+GGG - chr6.2:75984510-75984529 None:intergenic 25.0%
! ATGTGAGAATATAGCTATAA+GGG + chr6.2:75981485-75981504 MS.gene40442:CDS 25.0%
! GATAACAGTAATAATCTTCA+AGG + chr6.2:75984812-75984831 MS.gene40442:CDS 25.0%
! GATTATTACTGTTATCAAGT+TGG - chr6.2:75984808-75984827 None:intergenic 25.0%
! GTAAACTACTAACCATAATT+TGG - chr6.2:75984928-75984947 None:intergenic 25.0%
! TATAGAAATGCTATCTATAG+TGG + chr6.2:75983315-75983334 MS.gene40442:CDS 25.0%
! TCAATTTCTTCAAAGATGAA+AGG + chr6.2:75984025-75984044 MS.gene40442:CDS 25.0%
!! TGTATATTTTATAGGTCACT+TGG + chr6.2:75983247-75983266 MS.gene40442:intron 25.0%
!!! AGTTTTATTAGTTTCTCTCA+CGG + chr6.2:75981054-75981073 MS.gene40442:intron 25.0%
!!! ATGTACAATTTTAGTACCTA+AGG + chr6.2:75981755-75981774 MS.gene40442:intron 25.0%
!!! ATTCTAAGGTTATATTACCT+TGG - chr6.2:75983176-75983195 None:intergenic 25.0%
!!! GTTATTTTACAATGGTTTTG+TGG + chr6.2:75981387-75981406 MS.gene40442:intron 25.0%
!!! GTTTCTTTGTTTTTGTTTAG+TGG + chr6.2:75984134-75984153 MS.gene40442:intron 25.0%
!!! TGTATATGGTTCTAAATTAC+AGG - chr6.2:75983687-75983706 None:intergenic 25.0%
!!! TTTTTTCTTCAGTATGTTCA+AGG + chr6.2:75980856-75980875 MS.gene40442:intron 25.0%
AAATTGAAAACAGTTTGTGG+AGG - chr6.2:75984012-75984031 None:intergenic 30.0%
AACAGTTGCATTCCAAATTA+TGG + chr6.2:75984913-75984932 MS.gene40442:CDS 30.0%
AACCAAAATTTGATCTGTCT+AGG - chr6.2:75980941-75980960 None:intergenic 30.0%
AAGAAGTAGAATTCTCTGAT+AGG - chr6.2:75983007-75983026 None:intergenic 30.0%
AAGACAAAAATCCAGTTCAT+AGG + chr6.2:75983289-75983308 MS.gene40442:CDS 30.0%
AATTGAAAACAGTTTGTGGA+GGG - chr6.2:75984011-75984030 None:intergenic 30.0%
ACCAATTCACATTTCATCAT+TGG + chr6.2:75982217-75982236 MS.gene40442:CDS 30.0%
ACTATTGAAGTTATCTCTTC+TGG + chr6.2:75982765-75982784 MS.gene40442:CDS 30.0%
AGTAATAATCTTCAAGGAAG+TGG + chr6.2:75984818-75984837 MS.gene40442:CDS 30.0%
ATTTCCTACTATTGTGACTA+TGG + chr6.2:75982737-75982756 MS.gene40442:CDS 30.0%
GATGTGAGAATATAGCTATA+AGG + chr6.2:75981484-75981503 MS.gene40442:CDS 30.0%
TATTATGAATGGCTTAGCTT+TGG + chr6.2:75982577-75982596 MS.gene40442:CDS 30.0%
TCATCATTGGACTTATCAAT+GGG + chr6.2:75982230-75982249 MS.gene40442:CDS 30.0%
TCCAATGATGAAATGTGAAT+TGG - chr6.2:75982221-75982240 None:intergenic 30.0%
TGTTCCCTAAGACTAATAAT+CGG + chr6.2:75982504-75982523 MS.gene40442:CDS 30.0%
TTCATCATTGGACTTATCAA+TGG + chr6.2:75982229-75982248 MS.gene40442:CDS 30.0%
TTTCTATAAAGCCTATGAAC+TGG - chr6.2:75983303-75983322 None:intergenic 30.0%
TTTGTGCAATTATAGTCCTT+AGG - chr6.2:75981774-75981793 None:intergenic 30.0%
! AAACAATCATCATTGATTGG+AGG - chr6.2:75981549-75981568 None:intergenic 30.0%
! AGCTTTGGTTGAAATGTTTA+TGG + chr6.2:75982592-75982611 MS.gene40442:CDS 30.0%
! GACTTATCAATGGGAAATTT+TGG + chr6.2:75982239-75982258 MS.gene40442:CDS 30.0%
! TCTCGTGTTGTTATTATGAA+TGG + chr6.2:75982566-75982585 MS.gene40442:CDS 30.0%
! TTATCAATGGGAAATTTTGG+TGG + chr6.2:75982242-75982261 MS.gene40442:CDS 30.0%
! TTTAGTTGCATTTTACTGCA+AGG + chr6.2:75983579-75983598 MS.gene40442:intron 30.0%
!! TATCAATGGGAAATTTTGGT+GGG + chr6.2:75982243-75982262 MS.gene40442:CDS 30.0%
!! TCAACCGATTATTAGTCTTA+GGG - chr6.2:75982511-75982530 None:intergenic 30.0%
!! TTCAACCGATTATTAGTCTT+AGG - chr6.2:75982512-75982531 None:intergenic 30.0%
!!! AATTTTGGTTTCAGAACAGA+AGG + chr6.2:75980951-75980970 MS.gene40442:CDS 30.0%
!!! CATCAAAGTCCGTTATTTTT+TGG + chr6.2:75981939-75981958 MS.gene40442:intron 30.0%
!!! TCATCCACTTTGTTTTCAAT+CGG + chr6.2:75984277-75984296 MS.gene40442:intron 30.0%
!!! TGGTTTTGGTTAAACATTAC+AGG + chr6.2:75981407-75981426 MS.gene40442:intron 30.0%
!!! TTACAATGGTTTTGTGGTTT+TGG + chr6.2:75981393-75981412 MS.gene40442:intron 30.0%
!!! TTTTAATTTGCAATCCGCAA+TGG - chr6.2:75982050-75982069 None:intergenic 30.0%
AAATGTGCAACTCAAAGAAG+AGG + chr6.2:75981596-75981615 MS.gene40442:CDS 35.0%
AATTCTCTGATAGGAACAAG+TGG - chr6.2:75982998-75983017 None:intergenic 35.0%
ACACTGCTAATCAGATTCAA+AGG + chr6.2:75980686-75980705 MS.gene40442:CDS 35.0%
ACCATCTGCTAAACTTAACA+TGG + chr6.2:75984946-75984965 MS.gene40442:CDS 35.0%
AGAAGATCAAGATCAAGTGA+AGG - chr6.2:75982287-75982306 None:intergenic 35.0%
AGATGAAAGGAAAAGATCAC+AGG + chr6.2:75984038-75984057 MS.gene40442:CDS 35.0%
ATATATGCAGTCTGCAATCA+CGG + chr6.2:75983492-75983511 MS.gene40442:intron 35.0%
ATCGAAATTGAGGAGCAAAT+TGG - chr6.2:75983102-75983121 None:intergenic 35.0%
ATGCAATGGAAGAGATGATT+AGG + chr6.2:75982399-75982418 MS.gene40442:CDS 35.0%
GAACAAGGATAAGTACTTAC+TGG - chr6.2:75982620-75982639 None:intergenic 35.0%
GAAGCTTCAATGCGAATATT+CGG - chr6.2:75982539-75982558 None:intergenic 35.0%
GAAGTTGCTCATATTGCAAA+TGG + chr6.2:75984187-75984206 MS.gene40442:CDS 35.0%
GCAAATTGAGCAAGGTTTAT+GGG + chr6.2:75983039-75983058 MS.gene40442:CDS 35.0%
GTTGTCATGAGGAAAATCAT+AGG - chr6.2:75983080-75983099 None:intergenic 35.0%
TAGTGGGATGTTCTTTCTAA+TGG + chr6.2:75984151-75984170 MS.gene40442:intron 35.0%
TCAATGAATGTAGCAATGAG+TGG + chr6.2:75984722-75984741 MS.gene40442:CDS 35.0%
TCCATGTTAAGTTTAGCAGA+TGG - chr6.2:75984950-75984969 None:intergenic 35.0%
TGCTGTAAACCTTTATCTTG+CGG + chr6.2:75981882-75981901 MS.gene40442:intron 35.0%
TGGATGAAATCAAACTCTGA+TGG + chr6.2:75982446-75982465 MS.gene40442:CDS 35.0%
TTAAGCGGAAAACATGAACT+CGG - chr6.2:75984404-75984423 None:intergenic 35.0%
TTCATTCAAGACATGCCTAA+TGG + chr6.2:75983145-75983164 MS.gene40442:CDS 35.0%
! AACATTTTGCCGCAAGATAA+AGG - chr6.2:75981894-75981913 None:intergenic 35.0%
! AGTGGTAATGTTAATGCTGA+TGG + chr6.2:75984836-75984855 MS.gene40442:CDS 35.0%
! CTCCTAGACAGATCAAATTT+TGG + chr6.2:75980936-75980955 MS.gene40442:CDS 35.0%
! GTTAAGCAGAGAATTAGCTT+TGG + chr6.2:75980913-75980932 MS.gene40442:CDS 35.0%
!! ACACTTTCAGCTTTGAATGA+GGG + chr6.2:75983898-75983917 MS.gene40442:CDS 35.0%
!! AGTTATCTCTTCTGGAATGA+TGG + chr6.2:75982773-75982792 MS.gene40442:CDS 35.0%
!! GATTGATTTTTCAGAACCAG+TGG + chr6.2:75982706-75982725 MS.gene40442:intron 35.0%
!! GATTGGTCTTCCAATGTATT+TGG - chr6.2:75982177-75982196 None:intergenic 35.0%
!! GCAACTGTTAATGGACTTAT+TGG + chr6.2:75984977-75984996 MS.gene40442:CDS 35.0%
!! GTTATCTCTTCTGGAATGAT+GGG + chr6.2:75982774-75982793 MS.gene40442:CDS 35.0%
AATTTGAACACGTGACCTCA+GGG - chr6.2:75984348-75984367 None:intergenic 40.0%
AGCATTGCTGCCAAATACAT+TGG + chr6.2:75982164-75982183 MS.gene40442:CDS 40.0%
AGCTCAAACCTAGGTAACAA+TGG + chr6.2:75984869-75984888 MS.gene40442:CDS 40.0%
ATATTCGCATTGAAGCTTCG+AGG + chr6.2:75982540-75982559 MS.gene40442:CDS 40.0%
ATTACCTTGGAGTAGCCATT+AGG - chr6.2:75983163-75983182 None:intergenic 40.0%
CAACTGCGGCCAAAAAATAA+CGG - chr6.2:75981951-75981970 None:intergenic 40.0%
CATCATCATCATCACCATGA+AGG + chr6.2:75980622-75980641 MS.gene40442:CDS 40.0%
CGCAAATTGAGCAAGGTTTA+TGG + chr6.2:75983038-75983057 MS.gene40442:CDS 40.0%
CTACTATTGTGACTATGGCA+AGG + chr6.2:75982742-75982761 MS.gene40442:CDS 40.0%
CTCATCCTGATGAGAAACAA+AGG + chr6.2:75980885-75980904 MS.gene40442:CDS 40.0%
CTCATTCAAAGCTGAAAGTG+TGG - chr6.2:75983899-75983918 None:intergenic 40.0%
CTCTTCAGAGAATGTGTGAA+AGG + chr6.2:75983370-75983389 MS.gene40442:CDS 40.0%
CTTGATCTTGATCTTCTACC+TGG + chr6.2:75982290-75982309 MS.gene40442:CDS 40.0%
CTTGCCATAGTCACAATAGT+AGG - chr6.2:75982744-75982763 None:intergenic 40.0%
GAAATGTGAATTGGTGCAAC+TGG - chr6.2:75982212-75982231 None:intergenic 40.0%
GAAGTGATTGTGAAACCTGA+AGG - chr6.2:75984776-75984795 None:intergenic 40.0%
GAATCTGATTAGCAGTGTGA+CGG - chr6.2:75980684-75980703 None:intergenic 40.0%
GAGAATCAGAAGAACCTTCA+TGG - chr6.2:75980639-75980658 None:intergenic 40.0%
GAGCTGATAACTGTCAACTA+AGG + chr6.2:75981442-75981461 MS.gene40442:CDS 40.0%
GAGTCTGTTGCAACTGTTAA+TGG + chr6.2:75984968-75984987 MS.gene40442:CDS 40.0%
GATACTTCAAGAAAGCTGTG+TGG + chr6.2:75984655-75984674 MS.gene40442:CDS 40.0%
GATCAACGCATTCGAAATTG+AGG + chr6.2:75981989-75982008 MS.gene40442:intron 40.0%
GCAATGTTATAATTGTGCCG+AGG - chr6.2:75984315-75984334 None:intergenic 40.0%
GGTCACTTGGATTGAACATT+TGG + chr6.2:75983260-75983279 MS.gene40442:intron 40.0%
GGTTTCACAATCACTTCTGA+TGG + chr6.2:75984779-75984798 MS.gene40442:CDS 40.0%
TACTGTACGCAAATTGAGCA+AGG + chr6.2:75983031-75983050 MS.gene40442:CDS 40.0%
TATGTGCAGCTACTACCATT+TGG + chr6.2:75983977-75983996 MS.gene40442:CDS 40.0%
TATTCGCATTGAAGCTTCGA+GGG + chr6.2:75982541-75982560 MS.gene40442:CDS 40.0%
TCAAACCTAGGTAACAATGG+TGG + chr6.2:75984872-75984891 MS.gene40442:CDS 40.0%
TGACATTGCATCGAATGCAA+TGG + chr6.2:75982385-75982404 MS.gene40442:CDS 40.0%
! AGGGGCAGATCACATTTTTA+AGG - chr6.2:75981515-75981534 None:intergenic 40.0%
! AGTATCATAGTGGAGCAAGT+GGG + chr6.2:75980584-75980603 MS.gene40442:CDS 40.0%
! ATCTATAGTGGAGCTGCATT+TGG + chr6.2:75983327-75983346 MS.gene40442:CDS 40.0%
! ATGATGATGATGATCACCAG+GGG - chr6.2:75980611-75980630 None:intergenic 40.0%
! ATGTTTTCCGCTTAACACAG+TGG + chr6.2:75984409-75984428 MS.gene40442:intron 40.0%
! CAAACAAAGCTTTGACCATG+AGG - chr6.2:75981331-75981350 None:intergenic 40.0%
! CTCACCGATTGAAAACAAAG+TGG - chr6.2:75984284-75984303 None:intergenic 40.0%
! CTTTGTTTCTCATCAGGATG+AGG - chr6.2:75980887-75980906 None:intergenic 40.0%
! GAGTTTGATTTCATCCACAG+AGG - chr6.2:75982443-75982462 None:intergenic 40.0%
! GATGATGATGATGATCACCA+GGG - chr6.2:75980612-75980631 None:intergenic 40.0%
! GCGGCAAAATGTTGTTGATT+CGG + chr6.2:75981901-75981920 MS.gene40442:intron 40.0%
! GGGGCAGATCACATTTTTAA+GGG - chr6.2:75981514-75981533 None:intergenic 40.0%
! TGAAAGGCTTGCTTGTTCTA+TGG + chr6.2:75983386-75983405 MS.gene40442:CDS 40.0%
! TGATGATGATGATGATCACC+AGG - chr6.2:75980613-75980632 None:intergenic 40.0%
! TGCAACCTTTGTTTCTCATC+AGG - chr6.2:75980893-75980912 None:intergenic 40.0%
!! AAATTTTGGTGGGCATGGAA+TGG + chr6.2:75982253-75982272 MS.gene40442:CDS 40.0%
!! CACACTTTCAGCTTTGAATG+AGG + chr6.2:75983897-75983916 MS.gene40442:CDS 40.0%
!! GATTTTTCAGAACCAGTGGA+TGG + chr6.2:75982710-75982729 MS.gene40442:intron 40.0%
!! GTTAGGAAGAGTACTGAACT+TGG + chr6.2:75983934-75983953 MS.gene40442:CDS 40.0%
!! TGAAGGTGTTACTGGAATGT+GGG - chr6.2:75984759-75984778 None:intergenic 40.0%
!! TGCATAGTTGATGAACTTCC+AGG - chr6.2:75982311-75982330 None:intergenic 40.0%
!!! CTTTGTTTTCAATCGGTGAG+AGG + chr6.2:75984284-75984303 MS.gene40442:intron 40.0%
!!! TTATTTTTTGGCCGCAGTTG+CGG + chr6.2:75981951-75981970 MS.gene40442:intron 40.0%
AAAACAGTTTGTGGAGGGAG+AGG - chr6.2:75984006-75984025 None:intergenic 45.0%
AACTGCAATCGTGGTTGCAT+CGG + chr6.2:75983551-75983570 MS.gene40442:intron 45.0%
ACCAGCTTGTCTTTCTGACA+TGG + chr6.2:75982346-75982365 MS.gene40442:CDS 45.0%
ACCAGTTGCATAGAACCACT+AGG - chr6.2:75982804-75982823 None:intergenic 45.0%
AGGGAGTCAGAGTTACTGTT+AGG + chr6.2:75983917-75983936 MS.gene40442:CDS 45.0%
AGTCTAAGCAACTGCAATCG+TGG + chr6.2:75983542-75983561 MS.gene40442:intron 45.0%
ATATACCTCCACCGAGATCA+CGG - chr6.2:75983422-75983441 None:intergenic 45.0%
ATGTGAATTGGTGCAACTGG+TGG - chr6.2:75982209-75982228 None:intergenic 45.0%
ATTGCAAATGGTTCTCACCC+TGG + chr6.2:75984199-75984218 MS.gene40442:CDS 45.0%
CAACTCCATCTCTGTGCTTA+GGG + chr6.2:75984222-75984241 MS.gene40442:CDS 45.0%
CACATTCCAGTAACACCTTC+AGG + chr6.2:75984758-75984777 MS.gene40442:CDS 45.0%
CACTTCCACCATTGTTACCT+AGG - chr6.2:75984880-75984899 None:intergenic 45.0%
GAAACAGTCTCTCCTTTAGC+AGG + chr6.2:75984371-75984390 MS.gene40442:intron 45.0%
GAAAGCTGTGTGGATTCATC+AGG + chr6.2:75984665-75984684 MS.gene40442:CDS 45.0%
GAACAGAAGGACTCAGATGA+AGG + chr6.2:75980964-75980983 MS.gene40442:CDS 45.0%
GAATTTGAACACGTGACCTC+AGG - chr6.2:75984349-75984368 None:intergenic 45.0%
GACTTGACCACTGTGTTAAG+CGG - chr6.2:75984419-75984438 None:intergenic 45.0%
GAGGTCACGTGTTCAAATTC+TGG + chr6.2:75984349-75984368 MS.gene40442:intron 45.0%
GATGAAGCTAGCACAAAGGA+TGG + chr6.2:75983825-75983844 MS.gene40442:CDS 45.0%
GCATCAACAAGCTCAAACCT+AGG + chr6.2:75984860-75984879 MS.gene40442:CDS 45.0%
GCATGATGAAGCTAGCACAA+AGG + chr6.2:75983821-75983840 MS.gene40442:CDS 45.0%
GGAGCAAATTGGTTGTCATG+AGG - chr6.2:75983091-75983110 None:intergenic 45.0%
GTGCATCAGCTAGTCATAGA+TGG + chr6.2:75983872-75983891 MS.gene40442:CDS 45.0%
TAGGAAATAGAGCCATCCAC+TGG - chr6.2:75982725-75982744 None:intergenic 45.0%
TCCATGTCAGAAAGACAAGC+TGG - chr6.2:75982350-75982369 None:intergenic 45.0%
TCTTACCCTAAGCACAGAGA+TGG - chr6.2:75984230-75984249 None:intergenic 45.0%
TCTTGAATGAAGCAGCCAGA+TGG - chr6.2:75983136-75983155 None:intergenic 45.0%
TGTCAGAAAGACAAGCTGGT+TGG - chr6.2:75982346-75982365 None:intergenic 45.0%
TTGCTACATAACCGCAACTG+CGG - chr6.2:75981965-75981984 None:intergenic 45.0%
! AGACGATGGCATCGAAATTG+AGG - chr6.2:75983112-75983131 None:intergenic 45.0%
! ATAAGGAGGGCACATGTACT+CGG - chr6.2:75984463-75984482 None:intergenic 45.0%
! CTAGGTAACAATGGTGGAAG+TGG + chr6.2:75984878-75984897 MS.gene40442:CDS 45.0%
! CTGCATTTGGAGCTGAAAGA+TGG + chr6.2:75983340-75983359 MS.gene40442:CDS 45.0%
! GAAAGACAAGCTGGTTGGTA+AGG - chr6.2:75982341-75982360 None:intergenic 45.0%
! GAGTATCATAGTGGAGCAAG+TGG + chr6.2:75980583-75980602 MS.gene40442:CDS 45.0%
! TCCTAGTGGTTCTATGCAAC+TGG + chr6.2:75982800-75982819 MS.gene40442:CDS 45.0%
! TTTCAGTTGCCCTAGTTTGC+TGG + chr6.2:75984555-75984574 MS.gene40442:intron 45.0%
!! ATCTCTTCTGGAATGATGGG+TGG + chr6.2:75982777-75982796 MS.gene40442:CDS 45.0%
!! ATGGGAAATTTTGGTGGGCA+TGG + chr6.2:75982248-75982267 MS.gene40442:CDS 45.0%
!! CTGAAGGTGTTACTGGAATG+TGG - chr6.2:75984760-75984779 None:intergenic 45.0%
!! GTGAAACCTGAAGGTGTTAC+TGG - chr6.2:75984767-75984786 None:intergenic 45.0%
!! AAGAAAAAAAATAAAAAATA+GGG - chr6.2:75980845-75980864 None:intergenic 5.0%
!! AATTAAAAATATATTTAACT+CGG + chr6.2:75981838-75981857 MS.gene40442:intron 5.0%
!! TTATTATTATTATAAAGTTT+AGG + chr6.2:75981190-75981209 MS.gene40442:intron 5.0%
AACGCTCCCACGTATAAGGA+GGG - chr6.2:75984476-75984495 None:intergenic 50.0%
AATGTGATCTGCCCCTCTTG+TGG + chr6.2:75981519-75981538 MS.gene40442:CDS 50.0%
ACATGTGCCCTCCTTATACG+TGG + chr6.2:75984466-75984485 MS.gene40442:intron 50.0%
ACTGAACTTGGTCAGCCTAG+TGG + chr6.2:75983946-75983965 MS.gene40442:CDS 50.0%
ACTGGTGGAAGCTGTGTGAT+TGG - chr6.2:75982194-75982213 None:intergenic 50.0%
ATCATGCTGCTCTTTCCCTC+AGG - chr6.2:75983808-75983827 None:intergenic 50.0%
ATCGGTGAGAGGATGTTCCT+CGG + chr6.2:75984295-75984314 MS.gene40442:intron 50.0%
ATTGGAGGACCACCACAAGA+GGG - chr6.2:75981534-75981553 None:intergenic 50.0%
ATTGGGTCCAAGTCTCCTCA+TGG + chr6.2:75981313-75981332 MS.gene40442:intron 50.0%
CATGAACGCTCCCACGTATA+AGG - chr6.2:75984480-75984499 None:intergenic 50.0%
CATGCCTAATGGCTACTCCA+AGG + chr6.2:75983156-75983175 MS.gene40442:CDS 50.0%
CATGTGCCCTCCTTATACGT+GGG + chr6.2:75984467-75984486 MS.gene40442:intron 50.0%
CTCGGTCTTACTCCTGCTAA+AGG - chr6.2:75984386-75984405 None:intergenic 50.0%
GCAACTCCATCTCTGTGCTT+AGG + chr6.2:75984221-75984240 MS.gene40442:CDS 50.0%
GCACATAGCACAACACCACT+AGG - chr6.2:75983964-75983983 None:intergenic 50.0%
GCTTTGACCATGAGGAGACT+TGG - chr6.2:75981323-75981342 None:intergenic 50.0%
TATACCTCCACCGAGATCAC+GGG - chr6.2:75983421-75983440 None:intergenic 50.0%
TCAGCGATTCCATCACCTGA+GGG + chr6.2:75983790-75983809 MS.gene40442:intron 50.0%
TCTGATTAGCAGTGTGACGG+TGG - chr6.2:75980681-75980700 None:intergenic 50.0%
TGCAGACTAATGAGCCTCTG+TGG + chr6.2:75982426-75982445 MS.gene40442:CDS 50.0%
TGCTGCTGTAGTTGCCATTG+CGG + chr6.2:75982033-75982052 MS.gene40442:intron 50.0%
TTCAGCGATTCCATCACCTG+AGG + chr6.2:75983789-75983808 MS.gene40442:intron 50.0%
! GGTAACAATGGTGGAAGTGG+AGG + chr6.2:75984881-75984900 MS.gene40442:CDS 50.0%
! TTTGGTGGGCATGGAATGGT+TGG + chr6.2:75982257-75982276 MS.gene40442:CDS 50.0%
AGCACAGAGATGGAGTTGCC+AGG - chr6.2:75984220-75984239 None:intergenic 55.0%
AGCAGCCAGATGGAAGACGA+TGG - chr6.2:75983126-75983145 None:intergenic 55.0%
CGATGCCATCGTCTTCCATC+TGG + chr6.2:75983118-75983137 MS.gene40442:CDS 55.0%
CTCGGTCTTTCTCCTGCTAG+AGG - chr6.2:75984445-75984464 None:intergenic 55.0%
CTGCTCTTTCCCTCAGGTGA+TGG - chr6.2:75983802-75983821 None:intergenic 55.0%
GAACGCTCCCACGTATAAGG+AGG - chr6.2:75984477-75984496 None:intergenic 55.0%
GATTGGAGGACCACCACAAG+AGG - chr6.2:75981535-75981554 None:intergenic 55.0%
GCACAGAGATGGAGTTGCCA+GGG - chr6.2:75984219-75984238 None:intergenic 55.0%
GCAGTCTGCAATCACGGTTG+CGG + chr6.2:75983498-75983517 MS.gene40442:intron 55.0%
GGTCAAGTCTCTCCTCTAGC+AGG + chr6.2:75984430-75984449 MS.gene40442:intron 55.0%
GTTTCAGCCACCCGTGATCT+CGG + chr6.2:75983408-75983427 MS.gene40442:CDS 55.0%
TGGAGGGAGAGGTAGCCAAA+TGG - chr6.2:75983995-75984014 None:intergenic 55.0%
TTGGAGGACCACCACAAGAG+GGG - chr6.2:75981533-75981552 None:intergenic 55.0%
!! CGTGGGAGCGTTCATGTACT+CGG + chr6.2:75984484-75984503 MS.gene40442:intron 55.0%
!! GGAATGATGGGTGGTCCTAG+TGG + chr6.2:75982786-75982805 MS.gene40442:CDS 55.0%
ATTGCCGCCTTGTGACCCTG+AGG + chr6.2:75984330-75984349 MS.gene40442:intron 60.0%
CACGTGACCTCAGGGTCACA+AGG - chr6.2:75984340-75984359 None:intergenic 60.0%
GGTGGGCATGGAATGGTTGG+AGG + chr6.2:75982260-75982279 MS.gene40442:CDS 60.0%
GTGACCTCAGGGTCACAAGG+CGG - chr6.2:75984337-75984356 None:intergenic 60.0%
GTGATCTGCCCCTCTTGTGG+TGG + chr6.2:75981522-75981541 MS.gene40442:CDS 60.0%
ACCTCCACCGAGATCACGGG+TGG - chr6.2:75983418-75983437 None:intergenic 65.0%
AGTGGAGCAAGTGGGTCCCC+TGG + chr6.2:75980592-75980611 MS.gene40442:CDS 65.0%
TCAGCCACCCGTGATCTCGG+TGG + chr6.2:75983411-75983430 MS.gene40442:CDS 65.0%
GCCACCCGTGATCTCGGTGG+AGG + chr6.2:75983414-75983433 MS.gene40442:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 75980580 75985048 75980580 ID=MS.gene40442
chr6.2 mRNA 75980580 75985048 75980580 ID=MS.gene40442.t1;Parent=MS.gene40442
chr6.2 exon 75980580 75980716 75980580 ID=MS.gene40442.t1.exon1;Parent=MS.gene40442.t1
chr6.2 CDS 75980580 75980716 75980580 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75980868 75980985 75980868 ID=MS.gene40442.t1.exon2;Parent=MS.gene40442.t1
chr6.2 CDS 75980868 75980985 75980868 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75981429 75981617 75981429 ID=MS.gene40442.t1.exon3;Parent=MS.gene40442.t1
chr6.2 CDS 75981429 75981617 75981429 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75982149 75982619 75982149 ID=MS.gene40442.t1.exon4;Parent=MS.gene40442.t1
chr6.2 CDS 75982149 75982619 75982149 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75982720 75982821 75982720 ID=MS.gene40442.t1.exon5;Parent=MS.gene40442.t1
chr6.2 CDS 75982720 75982821 75982720 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75982968 75983177 75982968 ID=MS.gene40442.t1.exon6;Parent=MS.gene40442.t1
chr6.2 CDS 75982968 75983177 75982968 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75983261 75983435 75983261 ID=MS.gene40442.t1.exon7;Parent=MS.gene40442.t1
chr6.2 CDS 75983261 75983435 75983261 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75983794 75984059 75983794 ID=MS.gene40442.t1.exon8;Parent=MS.gene40442.t1
chr6.2 CDS 75983794 75984059 75983794 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75984154 75984243 75984154 ID=MS.gene40442.t1.exon9;Parent=MS.gene40442.t1
chr6.2 CDS 75984154 75984243 75984154 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
chr6.2 exon 75984626 75985048 75984626 ID=MS.gene40442.t1.exon10;Parent=MS.gene40442.t1
chr6.2 CDS 75984626 75985048 75984626 ID=cds.MS.gene40442.t1;Parent=MS.gene40442.t1
Gene Sequence

>MS.gene40442

ATGGAGTATCATAGTGGAGCAAGTGGGTCCCCTGGTGATCATCATCATCATCATCACCATGAAGGTTCTTCTGATTCTCAGAGAAGAAAGAAACGTTACCACCGTCACACTGCTAATCAGATTCAAAGGCTTGAAGCGTAAGAGTCATTTTCTTTCTCTTGTTTTGTGTGTTTATTTCTCTCTCTATCTCTCTCTTTCTCTCTTTCTCAACATTAAAACATAAACTTTGTTTTTGCACCATTTTTTTATATTTTTTTAATAACCCTATTTTTTATTTTTTTTCTTCAGTATGTTCAAGGAGTGTCCTCATCCTGATGAGAAACAAAGGTTGCAGTTAAGCAGAGAATTAGCTTTGGCTCCTAGACAGATCAAATTTTGGTTTCAGAACAGAAGGACTCAGATGAAGGTCTTATTTATTTTTCACTTTTTTTAATTTTTTTTCTTTAATAAAATAAAGAAACATGAATTAATGAAAGTTTTATTAGTTTCTCTCACGGTCAAATTGCGTATTTTTTTTTCTGGTTTATTAATTAATGTTTAATTGTGTATTTTTTTATTATTTCCTGGTTTATTAATTAATGTGTATTTTATATTTATGTTGGTTTATTAATTATTATTATTATAAAGTTTGTGTATTTTTCTGGTTTATTAATTCATGTGTAATTGTGTATTTTATATTTATGTTGATTTATTAATTTTTATTATTATTATTATTATTATAAAGTTTAGGTGAATTGGGTCCAAGTCTCCTCATGGTCAAAGCTTTGTTTGTTTTATAAATATTATTATTATAATATTTATTTATGTGTTATTTTACAATGGTTTTGTGGTTTTGGTTAAACATTACAGGCACAACATGAAAGAGCTGATAACTGTCAACTAAGGCAAGAGAATGATAAAATAAGATGTGAGAATATAGCTATAAGGGAAGCCCTTAAAAATGTGATCTGCCCCTCTTGTGGTGGTCCTCCAATCAATGATGATTGTTTCTTTGATGAACAAAAGCTGAGAATTGAAAATGTGCAACTCAAAGAAGAGGTAAAAAAATCATTAACTAACTTTATGCAAAAAAATTATCTCTTATTAATTCTCATTTACTTGCATAGTTTTAAAGATTAAATATGATTTTAATACCTATAAAATCACAATTTTTAAAGTTAAGTTCTGTAAAAAAAATGTACAATTTTAGTACCTAAGGACTATAATTGCACAAACATTTATAGGGACTAAACTTTAAAAAGATCGTGATTTTTATAGGAATTAAAAATATATTTAACTCGGTCAAAATGTTGTAAACATTTATGCTGTAAACCTTTATCTTGCGGCAAAATGTTGTTGATTCGGTCAAAATTGCATAGACATCAAAGTCCGTTATTTTTTGGCCGCAGTTGCGGTTATGTAGCAATTGCGATCAACGCATTCGAAATTGAGGATACTTCAAAATCTTTTATATTGCTGCTGTAGTTGCCATTGCGGATTGCAAATTAAAACCATGTTTTATATTTTTTATTTCAATTTACTATGTACTTTTATATGGTTAATTGAAATGGTATTTTTTGAACAATGTAGCTTGATAGAGTATCAAGCATTGCTGCCAAATACATTGGAAGACCAATCACACAGCTTCCACCAGTTGCACCAATTCACATTTCATCATTGGACTTATCAATGGGAAATTTTGGTGGGCATGGAATGGTTGGAGGACCTTCACTTGATCTTGATCTTCTACCTGGAAGTTCATCAACTATGCAGAATATTCCTTACCAACCAGCTTGTCTTTCTGACATGGAAAAATCTCTCATGTCTGACATTGCATCGAATGCAATGGAAGAGATGATTAGGCTTCTGCAGACTAATGAGCCTCTGTGGATGAAATCAAACTCTGATGGTAAAGATGTTCTTAATCTTGAAAACTATGAAAGGATGTTCCCTAAGACTAATAATCGGTTGAAGAATCCGAATATTCGCATTGAAGCTTCGAGGGATTCTCGTGTTGTTATTATGAATGGCTTAGCTTTGGTTGAAATGTTTATGGATCCAGTAAGTACTTATCCTTGTTCCTTTTCATTTTCATTATTTTGTTTTTGAATTCATAACTTCAATTTAATTAACTTATGAAATTTCATGATTGATTTTTCAGAACCAGTGGATGGCTCTATTTCCTACTATTGTGACTATGGCAAGGACTATTGAAGTTATCTCTTCTGGAATGATGGGTGGTCCTAGTGGTTCTATGCAACTGGTATGTTAATAACACTAAAAATATTTTGACATCGGAAAAAATTTCTAGCATCGAATTAGTATACGATTTTTATGTTATATTCATGCCGATTTCTATTCAACATTGAATTATCTTAATGCGATTTTTGTTTGTTTTGAAAATTTTAGATGTATGAGGAATTGCAAATGCTGTCACCACTTGTTCCTATCAGAGAATTCTACTTCTTGCGTTACTGTACGCAAATTGAGCAAGGTTTATGGGCAATTGTTGATGTTTCCTATGATTTTCCTCATGACAACCAATTTGCTCCTCAATTTCGATGCCATCGTCTTCCATCTGGCTGCTTCATTCAAGACATGCCTAATGGCTACTCCAAGGTAATATAACCTTAGAATCAATACTTTATTAATTTGATTTCATTTCATTGATTCATATTGAGTTACTCTTGTATATTTTATAGGTCACTTGGATTGAACATTTGGAGATTGAAGACAAAAATCCAGTTCATAGGCTTTATAGAAATGCTATCTATAGTGGAGCTGCATTTGGAGCTGAAAGATGGCTCACTACTCTTCAGAGAATGTGTGAAAGGCTTGCTTGTTCTATGGTTTCAGCCACCCGTGATCTCGGTGGAGGTATATATACATATAATTAACATTGATATTTACAATTTTACATAGTATAGTTTTAAATATATGCAGTCTGCAATCACGGTTGCGGTCACGATATAACAGCTTTAGAAGTCTAAGCAACTGCAATCGTGGTTGCATCGGCTGAATTTAGTTGCATTTTACTGCAGCTTTAGAAGTCTAAGCAACTGCAATCGTGGTTGCATCGGCTGAATTTAGTTGCATTTTACTGCAAGGTTTTTAGCATAACTGCACCTGTAATTTAGAACCATATACATATTTATCAATGTATGAATTTCGATCATATTTATATTTAACTTTCTTGGCTTGTGATCTAAAAGTTGTGTATATCTGAATTTTTCAGCGATTCCATCACCTGAGGGAAAGAGCAGCATGATGAAGCTAGCACAAAGGATGGTAACTAATTTCTGTGCAAGCATAAGTGCATCAGCTAGTCATAGATGGACCACACTTTCAGCTTTGAATGAGGGAGTCAGAGTTACTGTTAGGAAGAGTACTGAACTTGGTCAGCCTAGTGGTGTTGTGCTATGTGCAGCTACTACCATTTGGCTACCTCTCCCTCCACAAACTGTTTTCAATTTCTTCAAAGATGAAAGGAAAAGATCACAGGTAATTTGTCATATACTTTAACTAATATTTGATTCTATGACCTTAGAATGAAAATTTTGTTCTTATTAATTTTTGTTTCTTTGTTTTTGTTTAGTGGGATGTTCTTTCTAATGGAAATGCTGTTCAAGAAGTTGCTCATATTGCAAATGGTTCTCACCCTGGCAACTCCATCTCTGTGCTTAGGGTAAGACTTGCTTCCTCCATTTTATTCATTTTTTCATCCACTTTGTTTTCAATCGGTGAGAGGATGTTCCTCGGCACAATTATAACATTGCCGCCTTGTGACCCTGAGGTCACGTGTTCAAATTCTGGAAACAGTCTCTCCTTTAGCAGGAGTAAGACCGAGTTCATGTTTTCCGCTTAACACAGTGGTCAAGTCTCTCCTCTAGCAGGAGAAAGACCGAGTACATGTGCCCTCCTTATACGTGGGAGCGTTCATGTACTCGGCCCATTTTTTAGTTGCAACATGTTTTTCAAAAGTTGTAACAAAAGTATTTTCAGTTGCCCTAGTTTGCTGGATTTAATTTCGTTTCTAATGTATTTTTGTGAATTTTCAAATGTAATAGGCTTTCAACACAAGTCAAAACAACATGTTGATACTTCAAGAAAGCTGTGTGGATTCATCAGGTTCAATTGTAGTCTATTGTCCAATTGAATTAACATCAATGAATGTAGCAATGAGTGGTGAAGACACTTCCCACATTCCAGTAACACCTTCAGGTTTCACAATCACTTCTGATGGAAACCAACTTGATAACAGTAATAATCTTCAAGGAAGTGGTAATGTTAATGCTGATGGAGCATCAACAAGCTCAAACCTAGGTAACAATGGTGGAAGTGGAGGATCATTGATAACAGTTGCATTCCAAATTATGGTTAGTAGTTTACCATCTGCTAAACTTAACATGGAGTCTGTTGCAACTGTTAATGGACTTATTGGTGAGACTGTTCAGCACATAAAAGCTGCTTTGAATTGCCCTAGTTCTTGA
Protein sequence

>MS.gene40442.t1

MEYHSGASGSPGDHHHHHHHEGSSDSQRRKKRYHRHTANQIQRLEAMFKECPHPDEKQRLQLSRELALAPRQIKFWFQNRRTQMKAQHERADNCQLRQENDKIRCENIAIREALKNVICPSCGGPPINDDCFFDEQKLRIENVQLKEELDRVSSIAAKYIGRPITQLPPVAPIHISSLDLSMGNFGGHGMVGGPSLDLDLLPGSSSTMQNIPYQPACLSDMEKSLMSDIASNAMEEMIRLLQTNEPLWMKSNSDGKDVLNLENYERMFPKTNNRLKNPNIRIEASRDSRVVIMNGLALVEMFMDPNQWMALFPTIVTMARTIEVISSGMMGGPSGSMQLMYEELQMLSPLVPIREFYFLRYCTQIEQGLWAIVDVSYDFPHDNQFAPQFRCHRLPSGCFIQDMPNGYSKVTWIEHLEIEDKNPVHRLYRNAIYSGAAFGAERWLTTLQRMCERLACSMVSATRDLGGAIPSPEGKSSMMKLAQRMVTNFCASISASASHRWTTLSALNEGVRVTVRKSTELGQPSGVVLCAATTIWLPLPPQTVFNFFKDERKRSQWDVLSNGNAVQEVAHIANGSHPGNSISVLRAFNTSQNNMLILQESCVDSSGSIVVYCPIELTSMNVAMSGEDTSHIPVTPSGFTITSDGNQLDNSNNLQGSGNVNADGASTSSNLGNNGGSGGSLITVAFQIMVSSLPSAKLNMESVATVNGLIGETVQHIKAALNCPSS