Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40664.t1 | Q9FVL0.1 | 94.4 | 160 | 1 | 1 | 1 | 152 | 1 | 160 | 8.10E-75 | 289.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40664.t1 | Q9FVL0 | 94.4 | 160 | 1 | 1 | 1 | 152 | 1 | 160 | 2.0e-77 | 289.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40664.t1 | I3SPW2 | 93.8 | 160 | 2 | 1 | 1 | 152 | 1 | 160 | 1.7e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050380 | MS.gene40664 | 0.806033 | 1.00E-49 | -1.69E-46 |
| MS.gene050926 | MS.gene40664 | 0.805937 | 1.05E-49 | -1.69E-46 |
| MS.gene054435 | MS.gene40664 | 0.811231 | 7.76E-51 | -1.69E-46 |
| MS.gene056040 | MS.gene40664 | -0.81497 | 1.17E-51 | -1.69E-46 |
| MS.gene056313 | MS.gene40664 | 0.819849 | 9.35E-53 | -1.69E-46 |
| MS.gene056647 | MS.gene40664 | 0.892904 | 1.03E-74 | -1.69E-46 |
| MS.gene057506 | MS.gene40664 | 0.829884 | 4.01E-55 | -1.69E-46 |
| MS.gene058170 | MS.gene40664 | 0.80238 | 5.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40664.t1 | MTR_4g068860 | 93.750 | 160 | 2 | 1 | 1 | 152 | 1 | 160 | 8.55e-106 | 299 |
| MS.gene40664.t1 | MTR_4g068870 | 79.333 | 150 | 23 | 1 | 8 | 149 | 48 | 197 | 2.47e-81 | 239 |
| MS.gene40664.t1 | MTR_4g068870 | 79.333 | 150 | 23 | 1 | 8 | 149 | 131 | 280 | 3.47e-80 | 239 |
| MS.gene40664.t1 | MTR_4g068870 | 84.404 | 109 | 17 | 0 | 41 | 149 | 1 | 109 | 5.33e-61 | 190 |
| MS.gene40664.t1 | MTR_4g068870 | 76.923 | 156 | 28 | 1 | 2 | 149 | 171 | 326 | 6.48e-80 | 240 |
| MS.gene40664.t1 | MTR_4g068870 | 76.923 | 156 | 27 | 2 | 3 | 149 | 7 | 162 | 1.40e-77 | 234 |
| MS.gene40664.t1 | MTR_4g068870 | 79.333 | 150 | 23 | 1 | 8 | 149 | 184 | 333 | 2.27e-79 | 239 |
| MS.gene40664.t1 | MTR_4g068870 | 76.923 | 156 | 27 | 2 | 3 | 149 | 7 | 162 | 2.23e-77 | 233 |
| MS.gene40664.t1 | MTR_0026s0210 | 78.667 | 150 | 24 | 1 | 8 | 149 | 184 | 333 | 6.48e-79 | 237 |
| MS.gene40664.t1 | MTR_0026s0210 | 77.852 | 149 | 25 | 1 | 9 | 149 | 14 | 162 | 4.74e-78 | 235 |
| MS.gene40664.t1 | MTR_4g068860 | 89.256 | 121 | 5 | 1 | 1 | 113 | 1 | 121 | 1.04e-72 | 214 |
| MS.gene40664.t1 | MTR_1g090810 | 47.651 | 149 | 69 | 2 | 9 | 148 | 4 | 152 | 3.59e-35 | 120 |
| MS.gene40664.t1 | MTR_1g011540 | 46.939 | 147 | 67 | 3 | 9 | 148 | 3 | 145 | 7.56e-34 | 116 |
| MS.gene40664.t1 | MTR_5g041610 | 47.297 | 148 | 66 | 3 | 9 | 148 | 3 | 146 | 1.36e-33 | 115 |
| MS.gene40664.t1 | MTR_5g081030 | 45.270 | 148 | 69 | 3 | 9 | 148 | 3 | 146 | 1.88e-33 | 115 |
| MS.gene40664.t1 | MTR_5g080440 | 45.270 | 148 | 69 | 3 | 9 | 148 | 3 | 146 | 5.39e-33 | 114 |
| MS.gene40664.t1 | MTR_5g081000 | 46.259 | 147 | 68 | 3 | 9 | 148 | 3 | 145 | 9.95e-33 | 113 |
| MS.gene40664.t1 | MTR_5g066070 | 45.270 | 148 | 69 | 3 | 9 | 148 | 3 | 146 | 7.81e-32 | 111 |
| MS.gene40664.t1 | MTR_1g090820 | 43.919 | 148 | 72 | 2 | 9 | 148 | 3 | 147 | 7.18e-30 | 106 |
| MS.gene40664.t1 | MTR_5g080400 | 43.624 | 149 | 72 | 3 | 9 | 149 | 3 | 147 | 8.24e-30 | 106 |
| MS.gene40664.t1 | MTR_1g049330 | 41.892 | 148 | 75 | 2 | 9 | 148 | 3 | 147 | 9.09e-29 | 103 |
| MS.gene40664.t1 | MTR_1g090820 | 46.296 | 108 | 55 | 1 | 41 | 148 | 1 | 105 | 1.00e-22 | 86.7 |
| MS.gene40664.t1 | MTR_5g080400 | 44.954 | 109 | 56 | 2 | 41 | 149 | 1 | 105 | 1.96e-22 | 86.3 |
| MS.gene40664.t1 | MTR_5g080900 | 39.456 | 147 | 64 | 4 | 9 | 148 | 3 | 131 | 2.76e-22 | 86.3 |
| MS.gene40664.t1 | MTR_5g066070 | 43.519 | 108 | 49 | 3 | 9 | 108 | 3 | 106 | 7.44e-19 | 77.0 |
| MS.gene40664.t1 | MTR_1g090820 | 40.741 | 108 | 53 | 2 | 9 | 108 | 3 | 107 | 1.11e-16 | 71.2 |
| MS.gene40664.t1 | MTR_7g110180 | 41.525 | 118 | 48 | 5 | 9 | 120 | 3 | 105 | 2.79e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40664.t1 | AT2G16060 | 75.163 | 153 | 30 | 1 | 8 | 152 | 8 | 160 | 9.84e-81 | 235 |
| MS.gene40664.t1 | AT3G10520 | 51.007 | 149 | 65 | 1 | 9 | 149 | 6 | 154 | 1.31e-46 | 149 |
Find 24 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACTACACCATATTTAAAA+TGG | 0.249717 | 4.1:-33560635 | None:intergenic |
| GGTGACAAAGTTTGCACTAT+TGG | 0.325692 | 4.1:+33560829 | MS.gene40664:intron |
| AAAAGATTCAAAAGTTCCTT+TGG | 0.334733 | 4.1:+33560041 | MS.gene40664:CDS |
| TTCTCTGACCGTGACTTTAC+CGG | 0.339739 | 4.1:-33560586 | None:intergenic |
| CCTTTAGAATATGGGCACTT+TGG | 0.384904 | 4.1:+33559742 | None:intergenic |
| TATAAACTAGAGAGTTTAAG+AGG | 0.396192 | 4.1:-33560960 | None:intergenic |
| AACTAGAGAGTTTAAGAGGA+AGG | 0.427518 | 4.1:-33560956 | None:intergenic |
| ATAGCCGGTGACCACATTTC+AGG | 0.433187 | 4.1:-33560872 | None:intergenic |
| GCAGTTCAATTGCGGAAATC+CGG | 0.465498 | 4.1:+33560567 | MS.gene40664:CDS |
| CCCCATGCATTCTTCATAGC+CGG | 0.478259 | 4.1:-33560887 | None:intergenic |
| TGCCATGTCTGTCTTTCTCA+TGG | 0.508395 | 4.1:+33560089 | MS.gene40664:CDS |
| CACCGGCTATGAAGAATGCA+TGG | 0.521161 | 4.1:+33560885 | MS.gene40664:CDS |
| GGGAGAAGCTTATGATCAGC+TGG | 0.524272 | 4.1:+33560907 | MS.gene40664:CDS |
| AAGCTCTTGTGGTGAAGTCA+TGG | 0.525782 | 4.1:+33559792 | MS.gene40664:CDS |
| ACCGGCTATGAAGAATGCAT+GGG | 0.592597 | 4.1:+33560886 | MS.gene40664:CDS |
| AGTACCTGAAATGTGGTCAC+CGG | 0.611768 | 4.1:+33560868 | MS.gene40664:CDS |
| GTGAATCAGCAGTTCAATTG+CGG | 0.612574 | 4.1:+33560559 | MS.gene40664:CDS |
| CATGGCATGAGGCTTGAGCT+TGG | 0.612875 | 4.1:-33560073 | None:intergenic |
| TGAAGAACAAGAAGCTCTTG+TGG | 0.616139 | 4.1:+33559781 | MS.gene40664:CDS |
| AAGAAGCAGTACCTGAAATG+TGG | 0.626355 | 4.1:+33560861 | MS.gene40664:CDS |
| GCGGAAATCCGGTAAAGTCA+CGG | 0.634999 | 4.1:+33560578 | MS.gene40664:CDS |
| AGAAAGACAGACATGGCATG+AGG | 0.665594 | 4.1:-33560084 | None:intergenic |
| CCGGCTATGAAGAATGCATG+GGG | 0.704265 | 4.1:+33560887 | MS.gene40664:CDS |
| TACCATGAGAAAGACAGACA+TGG | 0.720993 | 4.1:-33560091 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAGTATATTTTTTTTACT+AGG | + | chr4.1:33560688-33560707 | MS.gene40664:intron | 10.0% |
| !!! | TAAGTATATTTTTTTTACTA+GGG | + | chr4.1:33560689-33560708 | MS.gene40664:intron | 10.0% |
| !!! | TTTAATTTTTTGATTTTGTA+AGG | + | chr4.1:33560808-33560827 | MS.gene40664:intron | 10.0% |
| !! | TTACATTAAAATTTGACATA+AGG | + | chr4.1:33560744-33560763 | MS.gene40664:intron | 15.0% |
| !! | TATCATTTCTCATATTCAAA+TGG | - | chr4.1:33560314-33560333 | None:intergenic | 20.0% |
| !! | TTAAAATTTGACATAAGGTT+TGG | + | chr4.1:33560749-33560768 | MS.gene40664:intron | 20.0% |
| !!! | CTAATTCAGTAACTAAATTT+TGG | + | chr4.1:33560176-33560195 | MS.gene40664:intron | 20.0% |
| ! | AAAAGATTCAAAAGTTCCTT+TGG | + | chr4.1:33560041-33560060 | MS.gene40664:CDS | 25.0% |
| ! | AATAAGAAGTGAGAAAAACA+AGG | + | chr4.1:33560438-33560457 | MS.gene40664:intron | 25.0% |
| ! | CTACTACACCATATTTAAAA+TGG | - | chr4.1:33560638-33560657 | None:intergenic | 25.0% |
| ! | GAATCAAGCTTGAAAAAATT+AGG | + | chr4.1:33560606-33560625 | MS.gene40664:CDS | 25.0% |
| ! | GCAATAACAAAATAATTAGC+TGG | - | chr4.1:33560533-33560552 | None:intergenic | 25.0% |
| ! | TTCTTATTGGTCAAAAACAA+TGG | - | chr4.1:33560426-33560445 | None:intergenic | 25.0% |
| ! | TTTAGTTACTGAATTAGAGA+CGG | - | chr4.1:33560173-33560192 | None:intergenic | 25.0% |
| !!! | TTGTTTTTCTCACTTCTTAT+TGG | - | chr4.1:33560439-33560458 | None:intergenic | 25.0% |
| AAAACAATGGAGAGAGAAAA+AGG | - | chr4.1:33560413-33560432 | None:intergenic | 30.0% | |
| GTCAAAAAGTTGTCAAAAAG+TGG | - | chr4.1:33560346-33560365 | None:intergenic | 30.0% | |
| TCAACAAAGTTGTCATCAAA+TGG | + | chr4.1:33560464-33560483 | MS.gene40664:intron | 30.0% | |
| ! | GGTGCTAACCATTTTAAATA+TGG | + | chr4.1:33560627-33560646 | MS.gene40664:CDS | 30.0% |
| AAATCCGTCTCTAATGTACT+CGG | - | chr4.1:33560203-33560222 | None:intergenic | 35.0% | |
| AAGAAGAATTCTGCAGAGTT+AGG | + | chr4.1:33559824-33559843 | MS.gene40664:intron | 35.0% | |
| TCTCATATTCAAATGGTGAG+TGG | - | chr4.1:33560307-33560326 | None:intergenic | 35.0% | |
| TGACAAAGTCAAAGCTTGAA+TGG | - | chr4.1:33560277-33560296 | None:intergenic | 35.0% | |
| !! | TTCAGGTACTGCTTCTTTTA+TGG | - | chr4.1:33560858-33560877 | None:intergenic | 35.0% |
| AAGAAGCAGTACCTGAAATG+TGG | + | chr4.1:33560861-33560880 | MS.gene40664:CDS | 40.0% | |
| AGAGAGAAAAAGGAAGAGAG+AGG | - | chr4.1:33560403-33560422 | None:intergenic | 40.0% | |
| GGTGACAAAGTTTGCACTAT+TGG | + | chr4.1:33560829-33560848 | MS.gene40664:intron | 40.0% | |
| GTGAATCAGCAGTTCAATTG+CGG | + | chr4.1:33560559-33560578 | MS.gene40664:CDS | 40.0% | |
| TACCATGAGAAAGACAGACA+TGG | - | chr4.1:33560094-33560113 | None:intergenic | 40.0% | |
| TGAAGAACAAGAAGCTCTTG+TGG | + | chr4.1:33559781-33559800 | MS.gene40664:CDS | 40.0% | |
| ! | TGTAGTAGACGAGCATTTTG+AGG | + | chr4.1:33560650-33560669 | MS.gene40664:CDS | 40.0% |
| !! | GAGAACAGTTTTTGAGCTGA+TGG | - | chr4.1:33560015-33560034 | None:intergenic | 40.0% |
| AAGCTCTTGTGGTGAAGTCA+TGG | + | chr4.1:33559792-33559811 | MS.gene40664:CDS | 45.0% | |
| ACCGGCTATGAAGAATGCAT+GGG | + | chr4.1:33560886-33560905 | MS.gene40664:CDS | 45.0% | |
| AGAAAGACAGACATGGCATG+AGG | - | chr4.1:33560087-33560106 | None:intergenic | 45.0% | |
| AGTACCTGAAATGTGGTCAC+CGG | + | chr4.1:33560868-33560887 | MS.gene40664:CDS | 45.0% | |
| AGTCAAAGCTTGAATGGTCG+TGG | - | chr4.1:33560271-33560290 | None:intergenic | 45.0% | |
| GCAGTTCAATTGCGGAAATC+CGG | + | chr4.1:33560567-33560586 | MS.gene40664:CDS | 45.0% | |
| TGCCATGTCTGTCTTTCTCA+TGG | + | chr4.1:33560089-33560108 | MS.gene40664:CDS | 45.0% | |
| TGGACCGAGTACATTAGAGA+CGG | + | chr4.1:33560196-33560215 | MS.gene40664:intron | 45.0% | |
| TTCTCTGACCGTGACTTTAC+CGG | - | chr4.1:33560589-33560608 | None:intergenic | 45.0% | |
| !!! | AGCTTGGTGTTTTGCTCCAA+AGG | - | chr4.1:33560060-33560079 | None:intergenic | 45.0% |
| ATAGCCGGTGACCACATTTC+AGG | - | chr4.1:33560875-33560894 | None:intergenic | 50.0% | |
| CCCCATGCATTCTTCATAGC+CGG | - | chr4.1:33560890-33560909 | None:intergenic | 50.0% | |
| CCGGCTATGAAGAATGCATG+GGG | + | chr4.1:33560887-33560906 | MS.gene40664:CDS | 50.0% | |
| GCGGAAATCCGGTAAAGTCA+CGG | + | chr4.1:33560578-33560597 | MS.gene40664:CDS | 50.0% | |
| GGGAGAAGCTTATGATCAGC+TGG | + | chr4.1:33560907-33560926 | MS.gene40664:CDS | 50.0% | |
| ! | CACCGGCTATGAAGAATGCA+TGG | + | chr4.1:33560885-33560904 | MS.gene40664:CDS | 50.0% |
| CATGGCATGAGGCTTGAGCT+TGG | - | chr4.1:33560076-33560095 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 33559752 | 33560967 | 33559752 | ID=MS.gene40664 |
| chr4.1 | mRNA | 33559752 | 33560967 | 33559752 | ID=MS.gene40664.t1;Parent=MS.gene40664 |
| chr4.1 | exon | 33559752 | 33559840 | 33559752 | ID=MS.gene40664.t1.exon1;Parent=MS.gene40664.t1 |
| chr4.1 | CDS | 33559752 | 33559840 | 33559752 | ID=cds.MS.gene40664.t1;Parent=MS.gene40664.t1 |
| chr4.1 | exon | 33559996 | 33560110 | 33559996 | ID=MS.gene40664.t1.exon2;Parent=MS.gene40664.t1 |
| chr4.1 | CDS | 33559996 | 33560110 | 33559996 | ID=cds.MS.gene40664.t1;Parent=MS.gene40664.t1 |
| chr4.1 | exon | 33560555 | 33560671 | 33560555 | ID=MS.gene40664.t1.exon3;Parent=MS.gene40664.t1 |
| chr4.1 | CDS | 33560555 | 33560671 | 33560555 | ID=cds.MS.gene40664.t1;Parent=MS.gene40664.t1 |
| chr4.1 | exon | 33560830 | 33560967 | 33560830 | ID=MS.gene40664.t1.exon4;Parent=MS.gene40664.t1 |
| chr4.1 | CDS | 33560830 | 33560967 | 33560830 | ID=cds.MS.gene40664.t1;Parent=MS.gene40664.t1 |
| Gene Sequence |
| Protein sequence |
