AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058170


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058170.t1 MTR_2g035190 95.541 157 7 0 1 157 1 157 3.48e-106 300
MS.gene058170.t1 MTR_2g035320 94.268 157 9 0 1 157 1 157 6.44e-106 299
MS.gene058170.t1 MTR_2g035210 89.809 157 16 0 1 157 1 157 1.82e-100 285
MS.gene058170.t1 MTR_2g035220 88.535 157 18 0 1 157 1 157 8.20e-98 278
MS.gene058170.t1 MTR_6g033450 66.879 157 52 0 1 157 1 157 5.58e-70 208
MS.gene058170.t1 MTR_4g120970 66.879 157 52 0 1 157 1 157 5.58e-70 208
MS.gene058170.t1 MTR_2g035100 62.420 157 59 0 1 157 1 157 6.74e-67 201
MS.gene058170.t1 MTR_2g035105 61.783 157 60 0 1 157 1 157 5.57e-66 198
MS.gene058170.t1 MTR_2g035150 62.420 157 59 0 1 157 1 157 8.08e-66 198
MS.gene058170.t1 MTR_2g035130 60.510 157 62 0 1 157 1 157 1.25e-64 195
MS.gene058170.t1 MTR_2g035120 57.962 157 66 0 1 157 1 157 2.20e-61 186
MS.gene058170.t1 MTR_2g035320 88.000 100 12 0 58 157 23 122 2.80e-60 182
MS.gene058170.t1 MTR_2g035170 47.771 157 82 0 1 157 1 157 1.18e-48 154
MS.gene058170.t1 MTR_4g120950 41.615 161 87 5 1 157 1 158 1.82e-31 110
MS.gene058170.t1 MTR_4g120760 33.121 157 104 1 1 156 1 157 2.58e-29 105
MS.gene058170.t1 MTR_4g120760 33.121 157 104 1 1 156 1 157 2.58e-29 105
MS.gene058170.t1 MTR_2g435310 31.410 156 106 1 1 155 1 156 2.60e-29 105
MS.gene058170.t1 MTR_4g120940 63.014 73 27 0 85 157 47 119 1.41e-23 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTAATTTGCTAGTAATT+AGG 0.154148 2.2:+53716620 None:intergenic
GTTTAATTTGCTAGTAATTA+GGG 0.258519 2.2:+53716621 None:intergenic
GGATAGCTTCTTAATAGTTC+CGG 0.311830 2.2:+53717509 None:intergenic
TACAGCTTAGTGGGAGGAAC+AGG 0.324066 2.2:-53716836 MS.gene058170:CDS
CTTGATAAGTCCAGTTCCTT+TGG 0.329858 2.2:+53716670 None:intergenic
TTCAACAATTTCAACACTTT+GGG 0.350751 2.2:+53717545 None:intergenic
CTTCAACAATTTCAACACTT+TGG 0.364915 2.2:+53717544 None:intergenic
TGGGCAGCAGAAATCACCTT+TGG 0.387864 2.2:+53717564 None:intergenic
AGATAGAGTTGCATCACCTT+TGG 0.394288 2.2:+53716718 None:intergenic
GATAGCTTCTTAATAGTTCC+GGG 0.397748 2.2:+53717510 None:intergenic
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2.2:-53717537 MS.gene058170:CDS
GAGACTAAGGCCAAAGGAAC+TGG 0.415366 2.2:-53716680 MS.gene058170:CDS
AGAGCCTTGTAGAGTTTAGC+TGG 0.426244 2.2:+53717612 None:intergenic
GGGCAGCAGAAATCACCTTT+GGG 0.449440 2.2:+53717565 None:intergenic
TGGATATAACTACAGCTTAG+TGG 0.476164 2.2:-53716846 MS.gene058170:CDS
AAAATTGTTGCTGGCTCTGA+TGG 0.476544 2.2:-53716776 MS.gene058170:CDS
GCAGTGGATGAGGCAAACTT+TGG 0.494243 2.2:-53716866 MS.gene058170:CDS
CAAATTAGATGCAGTGGATG+AGG 0.499478 2.2:-53716876 MS.gene058170:CDS
TAATTTGTGTAGCACAAAGT+TGG 0.501990 2.2:+53716892 None:intergenic
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2.2:-53717543 MS.gene058170:CDS
GGATATAACTACAGCTTAGT+GGG 0.506709 2.2:-53716845 MS.gene058170:CDS
ATCTGAATTACCTTCAACAA+TGG 0.509250 2.2:+53717488 None:intergenic
GGACTTATCAAGGCCATTGA+GGG 0.514208 2.2:-53716659 MS.gene058170:CDS
CAAAGGAACTGGACTTATCA+AGG 0.516806 2.2:-53716669 MS.gene058170:CDS
AAGAAGCTATCCATTGTTGA+AGG 0.523757 2.2:-53717498 MS.gene058170:intron
AGCAGTACGTGAGGAGACTA+AGG 0.536209 2.2:-53716693 MS.gene058170:CDS
GAGTTGCATCACCTTTGGTA+TGG 0.537356 2.2:+53716723 None:intergenic
TGCCACGACGTAGCCCTCAA+TGG 0.549026 2.2:+53716646 None:intergenic
GGCTCCAGCTAAACTCTACA+AGG 0.556705 2.2:-53717616 MS.gene058170:CDS
ATTGTTGCTGGCTCTGATGG+TGG 0.570543 2.2:-53716773 MS.gene058170:CDS
TGGACTTATCAAGGCCATTG+AGG 0.572485 2.2:-53716660 MS.gene058170:CDS
GTTGAAGGAAATGGAGGACC+CGG 0.599694 2.2:-53717528 MS.gene058170:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2.2:+53717620 None:intergenic
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2.2:-53717580 MS.gene058170:CDS
CTTAGTGGGAGGAACAGGGT+TGG 0.609437 2.2:-53716831 MS.gene058170:CDS
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2.2:-53717534 MS.gene058170:CDS
GCTACACAAATTAGATGCAG+TGG 0.633814 2.2:-53716882 MS.gene058170:CDS
TTTCACTGAAATCTTAACAA+CGG 0.637000 2.2:+53716748 None:intergenic
GGCCATTGAGGGCTACGTCG+TGG 0.646344 2.2:-53716648 MS.gene058170:CDS
TCAGTGAAATACCATACCAA+AGG 0.652605 2.2:-53716734 MS.gene058170:CDS
CGTGAGGAGACTAAGGCCAA+AGG 0.670663 2.2:-53716686 MS.gene058170:CDS
TCTATCTGAAGCAGTACGTG+AGG 0.676642 2.2:-53716702 MS.gene058170:CDS
ACAGCTTAGTGGGAGGAACA+GGG 0.680453 2.2:-53716835 MS.gene058170:CDS
TATAACTACAGCTTAGTGGG+AGG 0.697586 2.2:-53716842 MS.gene058170:CDS
TGACGAACATGTCTCAACCG+TGG 0.725590 2.2:-53717637 MS.gene058170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATATAAATAATTATAA+TGG - chr2.2:53717353-53717372 MS.gene058170:intron 0.0%
!!! AAATAAAAGTATAACATAAA+TGG + chr2.2:53717157-53717176 None:intergenic 10.0%
!!! TTTGTCAATTTTTAAATAAA+AGG + chr2.2:53716944-53716963 None:intergenic 10.0%
!!! TTTTATACCAAAAAAAAAAA+AGG - chr2.2:53717070-53717089 MS.gene058170:intron 10.0%
!!! TAATTTAACTTTCTTTTCTT+TGG + chr2.2:53717049-53717068 None:intergenic 15.0%
!!! TTAACTTCCTTTTTTTTTTT+TGG + chr2.2:53717080-53717099 None:intergenic 15.0%
!! AATAGTAATCCAATTCTATT+TGG - chr2.2:53717119-53717138 MS.gene058170:intron 20.0%
! AATAGAATCTTGAGAAATGA+AGG - chr2.2:53717180-53717199 MS.gene058170:intron 25.0%
! AGGGTTATGTCTAATAATTA+TGG - chr2.2:53717225-53717244 MS.gene058170:intron 25.0%
! ATAATTATTAGACATAACCC+TGG + chr2.2:53717226-53717245 None:intergenic 25.0%
! TATAATGGTTATATTGCAGA+TGG - chr2.2:53717368-53717387 MS.gene058170:intron 25.0%
! TTCAACAATTTCAACACTTT+GGG + chr2.2:53716746-53716765 None:intergenic 25.0%
! TTCAGATGATTATTGATACT+CGG - chr2.2:53717264-53717283 MS.gene058170:intron 25.0%
! TTTCACTGAAATCTTAACAA+CGG + chr2.2:53717543-53717562 None:intergenic 25.0%
!!! CATCTGAATCATTTTTTTGA+AGG + chr2.2:53717252-53717271 None:intergenic 25.0%
ATCTGAATTACCTTCAACAA+TGG + chr2.2:53716803-53716822 None:intergenic 30.0%
CATGACTATCCAAATAGAAT+TGG + chr2.2:53717131-53717150 None:intergenic 30.0%
CTTCAACAATTTCAACACTT+TGG + chr2.2:53716747-53716766 None:intergenic 30.0%
GTTGAAATTGTTGAAGGAAA+TGG - chr2.2:53716751-53716770 MS.gene058170:CDS 30.0%
! TAATTTGTGTAGCACAAAGT+TGG + chr2.2:53717399-53717418 None:intergenic 30.0%
! TTTGAGACAAAAATTGTTGC+TGG - chr2.2:53717503-53717522 MS.gene058170:CDS 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG - chr2.2:53716745-53716764 MS.gene058170:CDS 30.0%
AAGAAGCTATCCATTGTTGA+AGG - chr2.2:53716790-53716809 MS.gene058170:CDS 35.0%
ATAATACATAAGCTCGTTCC+AGG - chr2.2:53717205-53717224 MS.gene058170:intron 35.0%
GAAATTGTTGAAGGAAATGG+AGG - chr2.2:53716754-53716773 MS.gene058170:CDS 35.0%
GACAAACATGATGTGACAAA+TGG - chr2.2:53716958-53716977 MS.gene058170:intron 35.0%
GATAGCTTCTTAATAGTTCC+GGG + chr2.2:53716781-53716800 None:intergenic 35.0%
GGATAGCTTCTTAATAGTTC+CGG + chr2.2:53716782-53716801 None:intergenic 35.0%
GGATATAACTACAGCTTAGT+GGG - chr2.2:53717443-53717462 MS.gene058170:intron 35.0%
TAATACATAAGCTCGTTCCA+GGG - chr2.2:53717206-53717225 MS.gene058170:intron 35.0%
TCAGTGAAATACCATACCAA+AGG - chr2.2:53717554-53717573 MS.gene058170:CDS 35.0%
TGGATATAACTACAGCTTAG+TGG - chr2.2:53717442-53717461 MS.gene058170:intron 35.0%
! GTTGTACCGACAAATTTGAT+CGG + chr2.2:53716904-53716923 None:intergenic 35.0%
!! CGGTCTAAAACAATTTTACG+CGG + chr2.2:53716877-53716896 None:intergenic 35.0%
AGATAGAGTTGCATCACCTT+TGG + chr2.2:53717573-53717592 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG - chr2.2:53717619-53717638 MS.gene058170:CDS 40.0%
CAAATTAGATGCAGTGGATG+AGG - chr2.2:53717412-53717431 MS.gene058170:intron 40.0%
CGACAAATTTGATCGGTTGT+CGG + chr2.2:53716897-53716916 None:intergenic 40.0%
CGACAACCGATCAAATTTGT+CGG - chr2.2:53716895-53716914 MS.gene058170:CDS 40.0%
CTTGATAAGTCCAGTTCCTT+TGG + chr2.2:53717621-53717640 None:intergenic 40.0%
GCTACACAAATTAGATGCAG+TGG - chr2.2:53717406-53717425 MS.gene058170:intron 40.0%
TATAACTACAGCTTAGTGGG+AGG - chr2.2:53717446-53717465 MS.gene058170:intron 40.0%
!! AAAATTGTTGCTGGCTCTGA+TGG - chr2.2:53717512-53717531 MS.gene058170:CDS 40.0%
GAGTTGCATCACCTTTGGTA+TGG + chr2.2:53717568-53717587 None:intergenic 45.0%
GGACTTATCAAGGCCATTGA+GGG - chr2.2:53717629-53717648 MS.gene058170:CDS 45.0%
TGCTGATGAAATCGTCCCAA+AGG - chr2.2:53716708-53716727 MS.gene058170:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG - chr2.2:53717628-53717647 MS.gene058170:CDS 45.0%
! AGAGCCTTGTAGAGTTTAGC+TGG + chr2.2:53716679-53716698 None:intergenic 45.0%
! TCTATCTGAAGCAGTACGTG+AGG - chr2.2:53717586-53717605 MS.gene058170:CDS 45.0%
ACAGCTTAGTGGGAGGAACA+GGG - chr2.2:53717453-53717472 MS.gene058170:intron 50.0%
AGCAGTACGTGAGGAGACTA+AGG - chr2.2:53717595-53717614 MS.gene058170:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG - chr2.2:53717608-53717627 MS.gene058170:CDS 50.0%
GCAGTGGATGAGGCAAACTT+TGG - chr2.2:53717422-53717441 MS.gene058170:intron 50.0%
GGCTCCAGCTAAACTCTACA+AGG - chr2.2:53716672-53716691 MS.gene058170:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG + chr2.2:53716726-53716745 None:intergenic 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG + chr2.2:53716671-53716690 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG - chr2.2:53716760-53716779 MS.gene058170:CDS 50.0%
TACAGCTTAGTGGGAGGAAC+AGG - chr2.2:53717452-53717471 MS.gene058170:intron 50.0%
TGACGAACATGTCTCAACCG+TGG - chr2.2:53716651-53716670 MS.gene058170:CDS 50.0%
TGGGCAGCAGAAATCACCTT+TGG + chr2.2:53716727-53716746 None:intergenic 50.0%
!! ATTGTTGCTGGCTCTGATGG+TGG - chr2.2:53717515-53717534 MS.gene058170:CDS 50.0%
CGTGAGGAGACTAAGGCCAA+AGG - chr2.2:53717602-53717621 MS.gene058170:CDS 55.0%
CTTAGTGGGAGGAACAGGGT+TGG - chr2.2:53717457-53717476 MS.gene058170:intron 55.0%
TGCCACGACGTAGCCCTCAA+TGG + chr2.2:53717645-53717664 None:intergenic 60.0%
GGCCATTGAGGGCTACGTCG+TGG - chr2.2:53717640-53717659 MS.gene058170:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 53716631 53717679 53716631 ID=MS.gene058170
chr2.2 mRNA 53716631 53717679 53716631 ID=MS.gene058170.t1;Parent=MS.gene058170
chr2.2 exon 53717499 53717679 53717499 ID=MS.gene058170.t1.exon1;Parent=MS.gene058170.t1
chr2.2 CDS 53717499 53717679 53717499 ID=cds.MS.gene058170.t1;Parent=MS.gene058170.t1
chr2.2 exon 53716631 53716923 53716631 ID=MS.gene058170.t1.exon2;Parent=MS.gene058170.t1
chr2.2 CDS 53716631 53716923 53716631 ID=cds.MS.gene058170.t1;Parent=MS.gene058170.t1
Gene Sequence

>MS.gene058170

ATGGGTGTTTTTACTTTCAATGACGAACATGTCTCAACCGTGGCTCCAGCTAAACTCTACAAGGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCCGGAACTATTAAGAAGCTATCCATTGTTGAAGGTAATTCAGATCATATACTATGTAGAAATATATAATACTTGAGATCAAATGATACATAGTGTCCGCGTAAAATTGTTTTAGACCGACAACCGATCAAATTTGTCGGTACAACAAAGAATAGTGTAACTACCTTTTATTTAAAAATTGACAAACATGATGTGACAAATGGAAGAGTTGCAATTGATTAATAATTTGATTAAATGATTCACAAATATGTAAGTTAAATTCATTATACCAAAGAAAAGAAAGTTAAATTAATTTTATACCAAAAAAAAAAAAGGAAGTTAAATACAACTGTAGCAAAAAAAATAGTAATCCAATTCTATTTGGATAGTCATGAAGCCATTTATGTTATACTTTTATTTTAAAATAGAATCTTGAGAAATGAAGGAGATAATACATAAGCTCGTTCCAGGGTTATGTCTAATAATTATGGACCTTCAAAAAAATGATTCAGATGATTATTGATACTCGGTAAAGTTCAAATCGATCTTTAACATAAAGAGTTTTTAGTCAAACTTTACTTATATTGAAAAAAAAATATTATATAAATAATTATAATGGTTATATTGCAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATGCAGTGGATGAGGCAAACTTTGGATATAACTACAGCTTAGTGGGAGGAACAGGGTTGGATGAAAATTTAGAGAAAGTTGAATTTGAGACAAAAATTGTTGCTGGCTCTGATGGTGGATCCGTTGTTAAGATTTCAGTGAAATACCATACCAAAGGTGATGCAACTCTATCTGAAGCAGTACGTGAGGAGACTAAGGCCAAAGGAACTGGACTTATCAAGGCCATTGAGGGCTACGTCGTGGCAAACCCTAATTACTAG

Protein sequence

>MS.gene058170.t1

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQSVEIVEGNGGPGTIKKLSIVEDGKTNFVLHKLDAVDEANFGYNYSLVGGTGLDENLEKVEFETKIVAGSDGGSVVKISVKYHTKGDATLSEAVREETKAKGTGLIKAIEGYVVANPNY