AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41689


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41689.t1 MTR_7g085050 99.471 189 1 0 1 189 1 189 1.55e-137 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41689.t1 AT5G06660 83.069 189 28 2 5 189 8 196 4.05e-102 292
MS.gene41689.t1 AT3G12030 83.069 189 28 2 5 189 8 196 7.39e-95 274

Find 54 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGTTCTCTTTGTGGTATT+TGG 0.104469 7.2:+22595330 MS.gene41689:CDS
AAAAGGCTTGAATGGCAATT+TGG 0.263436 7.2:-22595388 None:intergenic
AAATTGCCATTCAAGCCTTT+TGG 0.268584 7.2:+22595390 MS.gene41689:CDS
TGGTTCTCTTTGTGGTATTT+GGG 0.271042 7.2:+22595331 MS.gene41689:CDS
AACAAATTTGCAGAAGTTCT+TGG 0.285687 7.2:+22595509 MS.gene41689:CDS
GAACTAGATCAACTGGTCTT+TGG 0.297367 7.2:-22595582 None:intergenic
CTTCATAGTCTCGAGTTTCT+TGG 0.298943 7.2:-22595181 None:intergenic
AACTAGATCAACTGGTCTTT+GGG 0.342623 7.2:-22595581 None:intergenic
CAAATTTGTTCTGATACTAA+TGG 0.343691 7.2:-22595496 None:intergenic
GGTTGCTCTGGTTCTCTTTG+TGG 0.348482 7.2:+22595323 MS.gene41689:CDS
CCGCCAAGGGGTGCAGGTGC+TGG 0.379709 7.2:+22595540 MS.gene41689:CDS
ACAAATTTGCAGAAGTTCTT+GGG 0.395345 7.2:+22595510 MS.gene41689:CDS
CTGGTCTTTGGGTCTGGCAT+TGG 0.395377 7.2:-22595570 None:intergenic
GCGAAGGTTCTTGTAAGAAT+TGG 0.409536 7.2:-22595139 None:intergenic
CATAACAGAACTAGATCAAC+TGG 0.415941 7.2:-22595589 None:intergenic
ATCCGGTGGTGTGGTTGCTC+TGG 0.434250 7.2:+22595311 MS.gene41689:CDS
TTTGCACCGCCAAGGGGTGC+AGG 0.441884 7.2:+22595534 MS.gene41689:CDS
TCTCTCTTCAAGTTTAAATC+CGG 0.442713 7.2:+22595294 MS.gene41689:CDS
CTTGGAAGCTTTGTCGATAG+AGG 0.450316 7.2:-22595163 None:intergenic
CTCGGATGGCCTCACTGTTG+TGG 0.453737 7.2:+22595056 MS.gene41689:CDS
CTGCTCAATTCGCTATTTGA+AGG 0.455366 7.2:+22595354 MS.gene41689:CDS
GATCAACTGGTCTTTGGGTC+TGG 0.458148 7.2:-22595576 None:intergenic
CCAGCACCTGCACCCCTTGG+CGG 0.467860 7.2:-22595540 None:intergenic
TCGGATGGCCTCACTGTTGT+GGG 0.468742 7.2:+22595057 MS.gene41689:CDS
CTTCTCAGATTTCAAGTACT+CGG 0.477023 7.2:+22595038 MS.gene41689:CDS
ACAAGCCCAAAAGGCTTGAA+TGG 0.484472 7.2:-22595396 None:intergenic
ATCTTCATAACAAGCCCAAA+AGG 0.485077 7.2:-22595405 None:intergenic
TTGTCTGTGAGGCCATCTCA+TGG 0.486509 7.2:+22595100 MS.gene41689:CDS
CTCTTCAAGTTTAAATCCGG+TGG 0.507122 7.2:+22595297 MS.gene41689:CDS
AGACCAGCACCTGCACCCCT+TGG 0.507589 7.2:-22595543 None:intergenic
CGAGTACTTGAAATCTGAGA+AGG 0.540066 7.2:-22595037 None:intergenic
AGTCACGACTAGATTCTTTG+AGG 0.544166 7.2:-22595269 None:intergenic
CAATTCGCTATTTGAAGGCA+AGG 0.549223 7.2:+22595359 MS.gene41689:CDS
TGTCTGTGAGGCCATCTCAT+GGG 0.550860 7.2:+22595101 MS.gene41689:CDS
CAAGTTTAAATCCGGTGGTG+TGG 0.561902 7.2:+22595302 MS.gene41689:CDS
AACCAGAGCAACCACACCAC+CGG 0.570086 7.2:-22595313 None:intergenic
TCAGATTTCAAGTACTCGGA+TGG 0.574798 7.2:+22595042 MS.gene41689:CDS
AAATGCCATGGAACAATCAG+TGG 0.579830 7.2:-22595457 None:intergenic
ATGAGCCATCGAGGGTTGCA+AGG 0.585212 7.2:+22595426 MS.gene41689:CDS
CTTTGTCGATAGAGGAGCGA+AGG 0.585269 7.2:-22595155 None:intergenic
CATTACCTTGCAACCCTCGA+TGG 0.592832 7.2:-22595431 None:intergenic
TGATCCCACTGATTGTTCCA+TGG 0.598157 7.2:+22595452 MS.gene41689:CDS
GTAAATAAGAACCCATGAGA+TGG 0.599701 7.2:-22595112 None:intergenic
TTCTGATACTAATGGAACAC+AGG 0.602611 7.2:-22595488 None:intergenic
CTGTACAGCTATTGTCTGTG+AGG 0.606267 7.2:+22595089 MS.gene41689:CDS
ATCTGAGAAGGGATATGCCA+TGG 0.607371 7.2:-22595025 None:intergenic
CAGGAAGTATAGAAATGCCA+TGG 0.612214 7.2:-22595469 None:intergenic
TTATGAAGATGAGCCATCGA+GGG 0.622078 7.2:+22595418 MS.gene41689:CDS
GAGTACTTGAAATCTGAGAA+GGG 0.629348 7.2:-22595036 None:intergenic
GGTTCTTGTAAGAATTGGTG+CGG 0.654185 7.2:-22595134 None:intergenic
CTTTGAGGCTAGTCTCAACA+CGG 0.657831 7.2:-22595254 None:intergenic
AATGCCATGGAACAATCAGT+GGG 0.668389 7.2:-22595456 None:intergenic
GTTATGAAGATGAGCCATCG+AGG 0.685544 7.2:+22595417 MS.gene41689:CDS
AAGAGATACCCACAACAGTG+AGG 0.718334 7.2:-22595065 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATTTGTTCTGATACTAA+TGG - chr7.2:22595499-22595518 None:intergenic 25.0%
AACAAATTTGCAGAAGTTCT+TGG + chr7.2:22595509-22595528 MS.gene41689:CDS 30.0%
ACAAATTTGCAGAAGTTCTT+GGG + chr7.2:22595510-22595529 MS.gene41689:CDS 30.0%
! TCTCTCTTCAAGTTTAAATC+CGG + chr7.2:22595294-22595313 MS.gene41689:CDS 30.0%
AAAAGGCTTGAATGGCAATT+TGG - chr7.2:22595391-22595410 None:intergenic 35.0%
ATCTTCATAACAAGCCCAAA+AGG - chr7.2:22595408-22595427 None:intergenic 35.0%
GAGTACTTGAAATCTGAGAA+GGG - chr7.2:22595039-22595058 None:intergenic 35.0%
GTAAATAAGAACCCATGAGA+TGG - chr7.2:22595115-22595134 None:intergenic 35.0%
TGGTTCTCTTTGTGGTATTT+GGG + chr7.2:22595331-22595350 MS.gene41689:CDS 35.0%
TTCTGATACTAATGGAACAC+AGG - chr7.2:22595491-22595510 None:intergenic 35.0%
! AAATTGCCATTCAAGCCTTT+TGG + chr7.2:22595390-22595409 MS.gene41689:CDS 35.0%
! AATTGCCATTCAAGCCTTTT+GGG + chr7.2:22595391-22595410 MS.gene41689:CDS 35.0%
! CTTCTCAGATTTCAAGTACT+CGG + chr7.2:22595038-22595057 MS.gene41689:CDS 35.0%
!!! CTCAACACGGTCAATTTTTT+TGG - chr7.2:22595244-22595263 None:intergenic 35.0%
AAATGCCATGGAACAATCAG+TGG - chr7.2:22595460-22595479 None:intergenic 40.0%
AATGCCATGGAACAATCAGT+GGG - chr7.2:22595459-22595478 None:intergenic 40.0%
AGTCACGACTAGATTCTTTG+AGG - chr7.2:22595272-22595291 None:intergenic 40.0%
CAATTCGCTATTTGAAGGCA+AGG + chr7.2:22595359-22595378 MS.gene41689:CDS 40.0%
CGAGTACTTGAAATCTGAGA+AGG - chr7.2:22595040-22595059 None:intergenic 40.0%
CTGCTCAATTCGCTATTTGA+AGG + chr7.2:22595354-22595373 MS.gene41689:CDS 40.0%
CTTCATAGTCTCGAGTTTCT+TGG - chr7.2:22595184-22595203 None:intergenic 40.0%
GCGAAGGTTCTTGTAAGAAT+TGG - chr7.2:22595142-22595161 None:intergenic 40.0%
GGTTCTTGTAAGAATTGGTG+CGG - chr7.2:22595137-22595156 None:intergenic 40.0%
TTATGAAGATGAGCCATCGA+GGG + chr7.2:22595418-22595437 MS.gene41689:CDS 40.0%
! CAGGAAGTATAGAAATGCCA+TGG - chr7.2:22595472-22595491 None:intergenic 40.0%
! CTCTTCAAGTTTAAATCCGG+TGG + chr7.2:22595297-22595316 MS.gene41689:CDS 40.0%
! CTGGTTCTCTTTGTGGTATT+TGG + chr7.2:22595330-22595349 MS.gene41689:CDS 40.0%
! TCAGATTTCAAGTACTCGGA+TGG + chr7.2:22595042-22595061 MS.gene41689:CDS 40.0%
AAGAGATACCCACAACAGTG+AGG - chr7.2:22595068-22595087 None:intergenic 45.0%
ACAAGCCCAAAAGGCTTGAA+TGG - chr7.2:22595399-22595418 None:intergenic 45.0%
CAAGTTTAAATCCGGTGGTG+TGG + chr7.2:22595302-22595321 MS.gene41689:CDS 45.0%
CTGTACAGCTATTGTCTGTG+AGG + chr7.2:22595089-22595108 MS.gene41689:CDS 45.0%
GTTATGAAGATGAGCCATCG+AGG + chr7.2:22595417-22595436 MS.gene41689:CDS 45.0%
! CTTGGAAGCTTTGTCGATAG+AGG - chr7.2:22595166-22595185 None:intergenic 45.0%
! TGATCCCACTGATTGTTCCA+TGG + chr7.2:22595452-22595471 MS.gene41689:CDS 45.0%
!! CTTTGAGGCTAGTCTCAACA+CGG - chr7.2:22595257-22595276 None:intergenic 45.0%
CATTACCTTGCAACCCTCGA+TGG - chr7.2:22595434-22595453 None:intergenic 50.0%
CTTTGTCGATAGAGGAGCGA+AGG - chr7.2:22595158-22595177 None:intergenic 50.0%
TGTCTGTGAGGCCATCTCAT+GGG + chr7.2:22595101-22595120 MS.gene41689:CDS 50.0%
TTGTCTGTGAGGCCATCTCA+TGG + chr7.2:22595100-22595119 MS.gene41689:CDS 50.0%
!! GGTTGCTCTGGTTCTCTTTG+TGG + chr7.2:22595323-22595342 MS.gene41689:CDS 50.0%
AACCAGAGCAACCACACCAC+CGG - chr7.2:22595316-22595335 None:intergenic 55.0%
ATGAGCCATCGAGGGTTGCA+AGG + chr7.2:22595426-22595445 MS.gene41689:CDS 55.0%
TCGGATGGCCTCACTGTTGT+GGG + chr7.2:22595057-22595076 MS.gene41689:CDS 55.0%
! CTGGTCTTTGGGTCTGGCAT+TGG - chr7.2:22595573-22595592 None:intergenic 55.0%
! CTTGGGTTTTGCACCGCCAA+GGG + chr7.2:22595527-22595546 MS.gene41689:CDS 55.0%
! TCTTGGGTTTTGCACCGCCA+AGG + chr7.2:22595526-22595545 MS.gene41689:CDS 55.0%
! TTGGGTTTTGCACCGCCAAG+GGG + chr7.2:22595528-22595547 MS.gene41689:CDS 55.0%
ATCCGGTGGTGTGGTTGCTC+TGG + chr7.2:22595311-22595330 MS.gene41689:CDS 60.0%
CTCGGATGGCCTCACTGTTG+TGG + chr7.2:22595056-22595075 MS.gene41689:CDS 60.0%
AGACCAGCACCTGCACCCCT+TGG - chr7.2:22595546-22595565 None:intergenic 65.0%
! TTTGCACCGCCAAGGGGTGC+AGG + chr7.2:22595534-22595553 MS.gene41689:CDS 65.0%
CCAGCACCTGCACCCCTTGG+CGG - chr7.2:22595543-22595562 None:intergenic 70.0%
! CCGCCAAGGGGTGCAGGTGC+TGG + chr7.2:22595540-22595559 MS.gene41689:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 22595027 22595596 22595027 ID=MS.gene41689
chr7.2 mRNA 22595027 22595596 22595027 ID=MS.gene41689.t1;Parent=MS.gene41689
chr7.2 exon 22595027 22595596 22595027 ID=MS.gene41689.t1.exon1;Parent=MS.gene41689.t1
chr7.2 CDS 22595027 22595596 22595027 ID=cds.MS.gene41689.t1;Parent=MS.gene41689.t1
Gene Sequence

>MS.gene41689

ATGGCATATCCCTTCTCAGATTTCAAGTACTCGGATGGCCTCACTGTTGTGGGTATCTCTTTCTGTACAGCTATTGTCTGTGAGGCCATCTCATGGGTTCTTATTTACCGCACCAATTCTTACAAGAACCTTCGCTCCTCTATCGACAAAGCTTCCAAGAAACTCGAGACTATGAAGACAGACAACAACAAAATCAACATTAAGAAGTCGAAGACCAAAAAAATTGACCGTGTTGAGACTAGCCTCAAAGAATCTAGTCGTGACTTGTCTCTCTTCAAGTTTAAATCCGGTGGTGTGGTTGCTCTGGTTCTCTTTGTGGTATTTGGGCTGCTCAATTCGCTATTTGAAGGCAAGGTAGTTGCCAAATTGCCATTCAAGCCTTTTGGGCTTGTTATGAAGATGAGCCATCGAGGGTTGCAAGGTAATGATCCCACTGATTGTTCCATGGCATTTCTATACTTCCTGTGTTCCATTAGTATCAGAACAAATTTGCAGAAGTTCTTGGGTTTTGCACCGCCAAGGGGTGCAGGTGCTGGTCTCTTTCCAATGCCAGACCCAAAGACCAGTTGA

Protein sequence

>MS.gene41689.t1

MAYPFSDFKYSDGLTVVGISFCTAIVCEAISWVLIYRTNSYKNLRSSIDKASKKLETMKTDNNKINIKKSKTKKIDRVETSLKESSRDLSLFKFKSGGVVALVLFVVFGLLNSLFEGKVVAKLPFKPFGLVMKMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS