AlfalfaGEDB | MS.gene51947

M. sativa cultivar XinJiangDaYe / MS.gene51947

KEGG: K21891
GO: GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0006873 GO:0006874 GO:0006875 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0016021 GO:0019725 GO:0022803 GO:0022857 GO:0022890 GO:0030003 GO:0030176 GO:0031224 GO:0031227 GO:0031300 GO:0031301 GO:0032469 GO:0042592 GO:0046873 GO:0048878 GO:0050801 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0072503 GO:0072507 GO:0098771 GO:0110165
PFAM: PF01956.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51947.t1	XP_003624576.1	99.5	189	1	0	1	189	1	189	1.30E-98	369

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51947.t1	Q19714	47.9	163	84	1	12	174	4	165	4.6e-36	152.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51947.t1	B7FGM9	99.5	189	1	0	1	189	1	189	9.4e-99	369.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049350	MS.gene51947	0.803777	2.97E-49	-1.69E-46
MS.gene049807	MS.gene51947	0.847719	9.68E-60	-1.69E-46
MS.gene051234	MS.gene51947	0.815725	7.98E-52	-1.69E-46
MS.gene052155	MS.gene51947	-0.810485	1.13E-50	-1.69E-46
MS.gene053144	MS.gene51947	0.813109	3.02E-51	-1.69E-46
MS.gene053536	MS.gene51947	0.81438	1.59E-51	-1.69E-46
MS.gene053538	MS.gene51947	0.821457	4.00E-53	-1.69E-46
MS.gene057102	MS.gene51947	0.820817	5.61E-53	-1.69E-46
MS.gene059424	MS.gene51947	0.818605	1.80E-52	-1.69E-46
MS.gene059900	MS.gene51947	-0.841443	4.74E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene067770	MS.gene51947	PPI
MS.gene51947	MS.gene51910	PPI
MS.gene41689	MS.gene51947	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51947.t1	MTR_7g085050	99.471	189	1	0	1	189	1	189	1.55e-137	382

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51947.t1	AT5G06660	83.069	189	28	2	5	189	8	196	4.05e-102	292
MS.gene51947.t1	AT3G12030	83.069	189	28	2	5	189	8	196	7.39e-95	274

Find 54 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGGTTCTCTTTGTGGTATT+TGG	0.104469	7.3:+23556201	MS.gene51947:CDS
AAAAGGCTTGAATGGCAATT+TGG	0.263436	7.3:-23556259	None:intergenic
AAATTGCCATTCAAGCCTTT+TGG	0.268584	7.3:+23556261	MS.gene51947:CDS
TGGTTCTCTTTGTGGTATTT+GGG	0.271042	7.3:+23556202	MS.gene51947:CDS
AACAAATTTGCAGAAGTTCT+TGG	0.285687	7.3:+23556380	MS.gene51947:CDS
GAACTAGATCAACTGGTCTT+TGG	0.297367	7.3:-23556453	None:intergenic
CTTCATAGTCTCGAGTTTCT+TGG	0.298943	7.3:-23556052	None:intergenic
AACTAGATCAACTGGTCTTT+GGG	0.342623	7.3:-23556452	None:intergenic
CAAATTTGTTCTGATACTAA+TGG	0.343691	7.3:-23556367	None:intergenic
GGTTGCTCTGGTTCTCTTTG+TGG	0.348482	7.3:+23556194	MS.gene51947:CDS
CCGCCAAGGGGTGCAGGTGC+TGG	0.379709	7.3:+23556411	MS.gene51947:CDS
ACAAATTTGCAGAAGTTCTT+GGG	0.395345	7.3:+23556381	MS.gene51947:CDS
CTGGTCTTTGGGTCTGGCAT+TGG	0.395377	7.3:-23556441	None:intergenic
GCGAAGGTTCTTGTAAGAAT+TGG	0.409536	7.3:-23556010	None:intergenic
CATAACAGAACTAGATCAAC+TGG	0.415941	7.3:-23556460	None:intergenic
ATCCGGTGGTGTGGTTGCTC+TGG	0.434250	7.3:+23556182	MS.gene51947:CDS
TTTGCACCGCCAAGGGGTGC+AGG	0.441884	7.3:+23556405	MS.gene51947:CDS
TCTCTCTTCAAGTTTAAATC+CGG	0.442713	7.3:+23556165	MS.gene51947:CDS
CTTGGAAGCTTTGTCGATAG+AGG	0.450316	7.3:-23556034	None:intergenic
CTCGGATGGCCTCACTGTTG+TGG	0.453737	7.3:+23555927	MS.gene51947:CDS
CTGCTCAATTCGCTATTTGA+AGG	0.455366	7.3:+23556225	MS.gene51947:CDS
GATCAACTGGTCTTTGGGTC+TGG	0.458148	7.3:-23556447	None:intergenic
CCAGCACCTGCACCCCTTGG+CGG	0.467860	7.3:-23556411	None:intergenic
TCGGATGGCCTCACTGTTGT+GGG	0.468742	7.3:+23555928	MS.gene51947:CDS
CTTCTCAGATTTCAAGTACT+CGG	0.477023	7.3:+23555909	MS.gene51947:CDS
ACAAGCCCAAAAGGCTTGAA+TGG	0.484472	7.3:-23556267	None:intergenic
ATCTTCATAACAAGCCCAAA+AGG	0.485077	7.3:-23556276	None:intergenic
TTGTCTGTGAGGCCATCTCA+TGG	0.486509	7.3:+23555971	MS.gene51947:CDS
CTCTTCAAGTTTAAATCCGG+TGG	0.507122	7.3:+23556168	MS.gene51947:CDS
AGACCAGCACCTGCACCCCT+TGG	0.507589	7.3:-23556414	None:intergenic
CGAGTACTTGAAATCTGAGA+AGG	0.540066	7.3:-23555908	None:intergenic
AGTCACGACTAGATTCTTTG+AGG	0.544166	7.3:-23556140	None:intergenic
CAATTCGCTATTTGAAGGCA+AGG	0.549223	7.3:+23556230	MS.gene51947:CDS
TGTCTGTGAGGCCATCTCAT+GGG	0.550860	7.3:+23555972	MS.gene51947:CDS
CAAGTTTAAATCCGGTGGTG+TGG	0.561902	7.3:+23556173	MS.gene51947:CDS
AACCAGAGCAACCACACCAC+CGG	0.570086	7.3:-23556184	None:intergenic
TCAGATTTCAAGTACTCGGA+TGG	0.574798	7.3:+23555913	MS.gene51947:CDS
AAATGCCATGGAACAATCAG+TGG	0.579830	7.3:-23556328	None:intergenic
ATGAGCCATCGAGGGTTGCA+AGG	0.585212	7.3:+23556297	MS.gene51947:CDS
CTTTGTCGATAGAGGAGCGA+AGG	0.585269	7.3:-23556026	None:intergenic
CATTACCTTGCAACCCTCGA+TGG	0.592832	7.3:-23556302	None:intergenic
TGATCCCACTGATTGTTCCA+TGG	0.598157	7.3:+23556323	MS.gene51947:CDS
GTAAATAAGAACCCATGAGA+TGG	0.599701	7.3:-23555983	None:intergenic
TTCTGATACTAATGGAACAC+AGG	0.602611	7.3:-23556359	None:intergenic
CTGTACAGCTATTGTCTGTG+AGG	0.606267	7.3:+23555960	MS.gene51947:CDS
ATCTGAGAAGGGATATGCCA+TGG	0.607371	7.3:-23555896	None:intergenic
CAGGAAGTATAGAAATGCCA+TGG	0.612214	7.3:-23556340	None:intergenic
TTATGAAGATGAGCCATCGA+GGG	0.622078	7.3:+23556289	MS.gene51947:CDS
GAGTACTTGAAATCTGAGAA+GGG	0.629348	7.3:-23555907	None:intergenic
GGTTCTTGTAAGAATTGGTG+CGG	0.654185	7.3:-23556005	None:intergenic
CTTTGAGGCTAGTCTCAACA+CGG	0.657831	7.3:-23556125	None:intergenic
AATGCCATGGAACAATCAGT+GGG	0.668389	7.3:-23556327	None:intergenic
GTTATGAAGATGAGCCATCG+AGG	0.685544	7.3:+23556288	MS.gene51947:CDS
AAGAGATACCCACAACAGTG+AGG	0.718334	7.3:-23555936	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAATTTGTTCTGATACTAA+TGG	-	chr7.3:23556370-23556389	None:intergenic	25.0%
	AACAAATTTGCAGAAGTTCT+TGG	+	chr7.3:23556380-23556399	MS.gene51947:CDS	30.0%
	ACAAATTTGCAGAAGTTCTT+GGG	+	chr7.3:23556381-23556400	MS.gene51947:CDS	30.0%
!	TCTCTCTTCAAGTTTAAATC+CGG	+	chr7.3:23556165-23556184	MS.gene51947:CDS	30.0%
	AAAAGGCTTGAATGGCAATT+TGG	-	chr7.3:23556262-23556281	None:intergenic	35.0%
	ATCTTCATAACAAGCCCAAA+AGG	-	chr7.3:23556279-23556298	None:intergenic	35.0%
	GAGTACTTGAAATCTGAGAA+GGG	-	chr7.3:23555910-23555929	None:intergenic	35.0%
	GTAAATAAGAACCCATGAGA+TGG	-	chr7.3:23555986-23556005	None:intergenic	35.0%
	TGGTTCTCTTTGTGGTATTT+GGG	+	chr7.3:23556202-23556221	MS.gene51947:CDS	35.0%
	TTCTGATACTAATGGAACAC+AGG	-	chr7.3:23556362-23556381	None:intergenic	35.0%
!	AAATTGCCATTCAAGCCTTT+TGG	+	chr7.3:23556261-23556280	MS.gene51947:CDS	35.0%
!	AATTGCCATTCAAGCCTTTT+GGG	+	chr7.3:23556262-23556281	MS.gene51947:CDS	35.0%
!	CTTCTCAGATTTCAAGTACT+CGG	+	chr7.3:23555909-23555928	MS.gene51947:CDS	35.0%
!!!	CTCAACACGGTCAATTTTTT+TGG	-	chr7.3:23556115-23556134	None:intergenic	35.0%
	AAATGCCATGGAACAATCAG+TGG	-	chr7.3:23556331-23556350	None:intergenic	40.0%
	AATGCCATGGAACAATCAGT+GGG	-	chr7.3:23556330-23556349	None:intergenic	40.0%
	AGTCACGACTAGATTCTTTG+AGG	-	chr7.3:23556143-23556162	None:intergenic	40.0%
	CAATTCGCTATTTGAAGGCA+AGG	+	chr7.3:23556230-23556249	MS.gene51947:CDS	40.0%
	CGAGTACTTGAAATCTGAGA+AGG	-	chr7.3:23555911-23555930	None:intergenic	40.0%
	CTGCTCAATTCGCTATTTGA+AGG	+	chr7.3:23556225-23556244	MS.gene51947:CDS	40.0%
	CTTCATAGTCTCGAGTTTCT+TGG	-	chr7.3:23556055-23556074	None:intergenic	40.0%
	GCGAAGGTTCTTGTAAGAAT+TGG	-	chr7.3:23556013-23556032	None:intergenic	40.0%
	GGTTCTTGTAAGAATTGGTG+CGG	-	chr7.3:23556008-23556027	None:intergenic	40.0%
	TTATGAAGATGAGCCATCGA+GGG	+	chr7.3:23556289-23556308	MS.gene51947:CDS	40.0%
!	CAGGAAGTATAGAAATGCCA+TGG	-	chr7.3:23556343-23556362	None:intergenic	40.0%
!	CTCTTCAAGTTTAAATCCGG+TGG	+	chr7.3:23556168-23556187	MS.gene51947:CDS	40.0%
!	CTGGTTCTCTTTGTGGTATT+TGG	+	chr7.3:23556201-23556220	MS.gene51947:CDS	40.0%
!	TCAGATTTCAAGTACTCGGA+TGG	+	chr7.3:23555913-23555932	MS.gene51947:CDS	40.0%
	AAGAGATACCCACAACAGTG+AGG	-	chr7.3:23555939-23555958	None:intergenic	45.0%
	ACAAGCCCAAAAGGCTTGAA+TGG	-	chr7.3:23556270-23556289	None:intergenic	45.0%
	CAAGTTTAAATCCGGTGGTG+TGG	+	chr7.3:23556173-23556192	MS.gene51947:CDS	45.0%
	CTGTACAGCTATTGTCTGTG+AGG	+	chr7.3:23555960-23555979	MS.gene51947:CDS	45.0%
	GTTATGAAGATGAGCCATCG+AGG	+	chr7.3:23556288-23556307	MS.gene51947:CDS	45.0%
!	CTTGGAAGCTTTGTCGATAG+AGG	-	chr7.3:23556037-23556056	None:intergenic	45.0%
!	TGATCCCACTGATTGTTCCA+TGG	+	chr7.3:23556323-23556342	MS.gene51947:CDS	45.0%
!!	CTTTGAGGCTAGTCTCAACA+CGG	-	chr7.3:23556128-23556147	None:intergenic	45.0%
	CATTACCTTGCAACCCTCGA+TGG	-	chr7.3:23556305-23556324	None:intergenic	50.0%
	CTTTGTCGATAGAGGAGCGA+AGG	-	chr7.3:23556029-23556048	None:intergenic	50.0%
	TGTCTGTGAGGCCATCTCAT+GGG	+	chr7.3:23555972-23555991	MS.gene51947:CDS	50.0%
	TTGTCTGTGAGGCCATCTCA+TGG	+	chr7.3:23555971-23555990	MS.gene51947:CDS	50.0%
!!	GGTTGCTCTGGTTCTCTTTG+TGG	+	chr7.3:23556194-23556213	MS.gene51947:CDS	50.0%
	AACCAGAGCAACCACACCAC+CGG	-	chr7.3:23556187-23556206	None:intergenic	55.0%
	ATGAGCCATCGAGGGTTGCA+AGG	+	chr7.3:23556297-23556316	MS.gene51947:CDS	55.0%
	TCGGATGGCCTCACTGTTGT+GGG	+	chr7.3:23555928-23555947	MS.gene51947:CDS	55.0%
!	CTGGTCTTTGGGTCTGGCAT+TGG	-	chr7.3:23556444-23556463	None:intergenic	55.0%
!	CTTGGGTTTTGCACCGCCAA+GGG	+	chr7.3:23556398-23556417	MS.gene51947:CDS	55.0%
!	TCTTGGGTTTTGCACCGCCA+AGG	+	chr7.3:23556397-23556416	MS.gene51947:CDS	55.0%
!	TTGGGTTTTGCACCGCCAAG+GGG	+	chr7.3:23556399-23556418	MS.gene51947:CDS	55.0%
	ATCCGGTGGTGTGGTTGCTC+TGG	+	chr7.3:23556182-23556201	MS.gene51947:CDS	60.0%
	CTCGGATGGCCTCACTGTTG+TGG	+	chr7.3:23555927-23555946	MS.gene51947:CDS	60.0%
	AGACCAGCACCTGCACCCCT+TGG	-	chr7.3:23556417-23556436	None:intergenic	65.0%
!	TTTGCACCGCCAAGGGGTGC+AGG	+	chr7.3:23556405-23556424	MS.gene51947:CDS	65.0%
	CCAGCACCTGCACCCCTTGG+CGG	-	chr7.3:23556414-23556433	None:intergenic	70.0%
!	CCGCCAAGGGGTGCAGGTGC+TGG	+	chr7.3:23556411-23556430	MS.gene51947:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	23555898	23556467	23555898	ID=MS.gene51947
chr7.3	mRNA	23555898	23556467	23555898	ID=MS.gene51947.t1;Parent=MS.gene51947
chr7.3	exon	23555898	23556467	23555898	ID=MS.gene51947.t1.exon1;Parent=MS.gene51947.t1
chr7.3	CDS	23555898	23556467	23555898	ID=cds.MS.gene51947.t1;Parent=MS.gene51947.t1

Gene Sequence

>MS.gene51947

ATGGCATATCCCTTCTCAGATTTCAAGTACTCGGATGGCCTCACTGTTGTGGGTATCTCTTTCTGTACAGCTATTGTCTGTGAGGCCATCTCATGGGTTCTTATTTACCGCACCAATTCTTACAAGAACCTTCGCTCCTCTATCGACAAAGCTTCCAAGAAACTCGAGACTATGAAGACAGACAACAACAAAATCAACATTAAGAAGTCGAAGACCAAAAAAATTGACCGTGTTGAGACTAGCCTCAAAGAATCTAGTCGTGACTTGTCTCTCTTCAAGTTTAAATCCGGTGGTGTGGTTGCTCTGGTTCTCTTTGTGGTATTTGGGCTGCTCAATTCGCTATTTGAAGGCAAGGTAGTTGCCAAATTGCCATTCAAGCCTTTTGGGCTTGTTATGAAGATGAGCCATCGAGGGTTGCAAGGTAATGATCCCACTGATTGTTCCATGGCATTTCTATACTTCCTGTGTTCCATTAGTATCAGAACAAATTTGCAGAAGTTCTTGGGTTTTGCACCGCCAAGGGGTGCAGGTGCTGGTCTCTTTCCAATGCCAGACCCAAAGACCAGTTGA

Protein sequence

>MS.gene51947.t1

MAYPFSDFKYSDGLTVVGISFCTAIVCEAISWVLIYRTNSYKNLRSSIDKASKKLETMKTDNNKINIKKSKTKKIDRVETSLKESSRDLSLFKFKSGGVVALVLFVVFGLLNSLFEGKVVAKLPFKPFGLVMKMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS