AlfalfaGEDB | MS.gene51910

M. sativa cultivar XinJiangDaYe / MS.gene51910

KEGG: K21891
GO: GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0006873 GO:0006874 GO:0006875 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0016021 GO:0019725 GO:0022803 GO:0022857 GO:0022890 GO:0030003 GO:0030176 GO:0031224 GO:0031227 GO:0031300 GO:0031301 GO:0032469 GO:0042592 GO:0046873 GO:0048878 GO:0050801 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0072503 GO:0072507 GO:0098771 GO:0110165
PFAM: PF01956.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51910.t1	XP_003624576.1	99.5	189	1	0	1	189	1	189	1.30E-98	369

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51910.t1	Q19714	47.9	163	84	1	12	174	4	165	4.6e-36	152.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51910.t1	B7FGM9	99.5	189	1	0	1	189	1	189	9.4e-99	369.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049807	MS.gene51910	0.831973	1.23E-55	-1.69E-46
MS.gene052155	MS.gene51910	-0.802804	4.71E-49	-1.69E-46
MS.gene053144	MS.gene51910	0.80868	2.75E-50	-1.69E-46
MS.gene053536	MS.gene51910	0.804141	2.49E-49	-1.69E-46
MS.gene053538	MS.gene51910	0.810658	1.03E-50	-1.69E-46
MS.gene057102	MS.gene51910	0.802938	4.42E-49	-1.69E-46
MS.gene059424	MS.gene51910	0.807882	4.07E-50	-1.69E-46
MS.gene059900	MS.gene51910	-0.829106	6.20E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene51947	MS.gene51910	PPI
MS.gene41689	MS.gene51910	PPI
MS.gene067770	MS.gene51910	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51910.t1	MTR_7g085050	99.471	189	1	0	1	189	1	189	1.55e-137	382

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51910.t1	AT5G06660	83.069	189	28	2	5	189	8	196	4.05e-102	292
MS.gene51910.t1	AT3G12030	83.069	189	28	2	5	189	8	196	7.39e-95	274

Find 54 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGGTTCTCTTTGTGGTATT+TGG	0.104469	7.4:+22260323	MS.gene51910:CDS
AAAAGGCTTGAATGGCAATT+TGG	0.263436	7.4:-22260381	None:intergenic
AAATTGCCATTCAAGCCTTT+TGG	0.268584	7.4:+22260383	MS.gene51910:CDS
TGGTTCTCTTTGTGGTATTT+GGG	0.271042	7.4:+22260324	MS.gene51910:CDS
AACAAATTTGCAGAAGTTCT+TGG	0.285687	7.4:+22260502	MS.gene51910:CDS
GAACTAGATCAACTGGTCTT+TGG	0.297367	7.4:-22260575	None:intergenic
CTTCATAGTCTCGAGTTTCT+TGG	0.298943	7.4:-22260174	None:intergenic
AACTAGATCAACTGGTCTTT+GGG	0.342623	7.4:-22260574	None:intergenic
CAAATTTGTTCTGATACTAA+TGG	0.343691	7.4:-22260489	None:intergenic
GGTTGCTCTGGTTCTCTTTG+TGG	0.348482	7.4:+22260316	MS.gene51910:CDS
CCGCCAAGGGGTGCAGGTGC+TGG	0.379709	7.4:+22260533	MS.gene51910:CDS
ACAAATTTGCAGAAGTTCTT+GGG	0.395345	7.4:+22260503	MS.gene51910:CDS
CTGGTCTTTGGGTCTGGCAT+TGG	0.395377	7.4:-22260563	None:intergenic
GCGAAGGTTCTTGTAAGAAT+TGG	0.409536	7.4:-22260132	None:intergenic
CATAACAGAACTAGATCAAC+TGG	0.415941	7.4:-22260582	None:intergenic
ATCCGGTGGTGTGGTTGCTC+TGG	0.434250	7.4:+22260304	MS.gene51910:CDS
TTTGCACCGCCAAGGGGTGC+AGG	0.441884	7.4:+22260527	MS.gene51910:CDS
TCTCTCTTCAAGTTTAAATC+CGG	0.442713	7.4:+22260287	MS.gene51910:CDS
CTTGGAAGCTTTGTCGATAG+AGG	0.450316	7.4:-22260156	None:intergenic
CTCGGATGGCCTCACTGTTG+TGG	0.453737	7.4:+22260049	MS.gene51910:CDS
CTGCTCAATTCGCTATTTGA+AGG	0.455366	7.4:+22260347	MS.gene51910:CDS
GATCAACTGGTCTTTGGGTC+TGG	0.458148	7.4:-22260569	None:intergenic
CCAGCACCTGCACCCCTTGG+CGG	0.467860	7.4:-22260533	None:intergenic
TCGGATGGCCTCACTGTTGT+GGG	0.468742	7.4:+22260050	MS.gene51910:CDS
CTTCTCAGATTTCAAGTACT+CGG	0.477023	7.4:+22260031	MS.gene51910:CDS
ACAAGCCCAAAAGGCTTGAA+TGG	0.484472	7.4:-22260389	None:intergenic
ATCTTCATAACAAGCCCAAA+AGG	0.485077	7.4:-22260398	None:intergenic
TTGTCTGTGAGGCCATCTCA+TGG	0.486509	7.4:+22260093	MS.gene51910:CDS
CTCTTCAAGTTTAAATCCGG+TGG	0.507122	7.4:+22260290	MS.gene51910:CDS
AGACCAGCACCTGCACCCCT+TGG	0.507589	7.4:-22260536	None:intergenic
CGAGTACTTGAAATCTGAGA+AGG	0.540066	7.4:-22260030	None:intergenic
AGTCACGACTAGATTCTTTG+AGG	0.544166	7.4:-22260262	None:intergenic
CAATTCGCTATTTGAAGGCA+AGG	0.549223	7.4:+22260352	MS.gene51910:CDS
TGTCTGTGAGGCCATCTCAT+GGG	0.550860	7.4:+22260094	MS.gene51910:CDS
CAAGTTTAAATCCGGTGGTG+TGG	0.561902	7.4:+22260295	MS.gene51910:CDS
AACCAGAGCAACCACACCAC+CGG	0.570086	7.4:-22260306	None:intergenic
TCAGATTTCAAGTACTCGGA+TGG	0.574798	7.4:+22260035	MS.gene51910:CDS
AAATGCCATGGAACAATCAG+TGG	0.579830	7.4:-22260450	None:intergenic
ATGAGCCATCGAGGGTTGCA+AGG	0.585212	7.4:+22260419	MS.gene51910:CDS
CTTTGTCGATAGAGGAGCGA+AGG	0.585269	7.4:-22260148	None:intergenic
CATTACCTTGCAACCCTCGA+TGG	0.592832	7.4:-22260424	None:intergenic
TGATCCCACTGATTGTTCCA+TGG	0.598157	7.4:+22260445	MS.gene51910:CDS
GTAAATAAGAACCCATGAGA+TGG	0.599701	7.4:-22260105	None:intergenic
TTCTGATACTAATGGAACAC+AGG	0.602611	7.4:-22260481	None:intergenic
CTGTACAGCTATTGTCTGTG+AGG	0.606267	7.4:+22260082	MS.gene51910:CDS
ATCTGAGAAGGGATATGCCA+TGG	0.607371	7.4:-22260018	None:intergenic
CAGGAAGTATAGAAATGCCA+TGG	0.612214	7.4:-22260462	None:intergenic
TTATGAAGATGAGCCATCGA+GGG	0.622078	7.4:+22260411	MS.gene51910:CDS
GAGTACTTGAAATCTGAGAA+GGG	0.629348	7.4:-22260029	None:intergenic
GGTTCTTGTAAGAATTGGTG+CGG	0.654185	7.4:-22260127	None:intergenic
CTTTGAGGCTAGTCTCAACA+CGG	0.657831	7.4:-22260247	None:intergenic
AATGCCATGGAACAATCAGT+GGG	0.668389	7.4:-22260449	None:intergenic
GTTATGAAGATGAGCCATCG+AGG	0.685544	7.4:+22260410	MS.gene51910:CDS
AAGAGATACCCACAACAGTG+AGG	0.718334	7.4:-22260058	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAATTTGTTCTGATACTAA+TGG	-	chr7.4:22260492-22260511	None:intergenic	25.0%
	AACAAATTTGCAGAAGTTCT+TGG	+	chr7.4:22260502-22260521	MS.gene51910:CDS	30.0%
	ACAAATTTGCAGAAGTTCTT+GGG	+	chr7.4:22260503-22260522	MS.gene51910:CDS	30.0%
!	TCTCTCTTCAAGTTTAAATC+CGG	+	chr7.4:22260287-22260306	MS.gene51910:CDS	30.0%
	AAAAGGCTTGAATGGCAATT+TGG	-	chr7.4:22260384-22260403	None:intergenic	35.0%
	ATCTTCATAACAAGCCCAAA+AGG	-	chr7.4:22260401-22260420	None:intergenic	35.0%
	GAGTACTTGAAATCTGAGAA+GGG	-	chr7.4:22260032-22260051	None:intergenic	35.0%
	GTAAATAAGAACCCATGAGA+TGG	-	chr7.4:22260108-22260127	None:intergenic	35.0%
	TGGTTCTCTTTGTGGTATTT+GGG	+	chr7.4:22260324-22260343	MS.gene51910:CDS	35.0%
	TTCTGATACTAATGGAACAC+AGG	-	chr7.4:22260484-22260503	None:intergenic	35.0%
!	AAATTGCCATTCAAGCCTTT+TGG	+	chr7.4:22260383-22260402	MS.gene51910:CDS	35.0%
!	AATTGCCATTCAAGCCTTTT+GGG	+	chr7.4:22260384-22260403	MS.gene51910:CDS	35.0%
!	CTTCTCAGATTTCAAGTACT+CGG	+	chr7.4:22260031-22260050	MS.gene51910:CDS	35.0%
!!!	CTCAACACGGTCAATTTTTT+TGG	-	chr7.4:22260237-22260256	None:intergenic	35.0%
	AAATGCCATGGAACAATCAG+TGG	-	chr7.4:22260453-22260472	None:intergenic	40.0%
	AATGCCATGGAACAATCAGT+GGG	-	chr7.4:22260452-22260471	None:intergenic	40.0%
	AGTCACGACTAGATTCTTTG+AGG	-	chr7.4:22260265-22260284	None:intergenic	40.0%
	CAATTCGCTATTTGAAGGCA+AGG	+	chr7.4:22260352-22260371	MS.gene51910:CDS	40.0%
	CGAGTACTTGAAATCTGAGA+AGG	-	chr7.4:22260033-22260052	None:intergenic	40.0%
	CTGCTCAATTCGCTATTTGA+AGG	+	chr7.4:22260347-22260366	MS.gene51910:CDS	40.0%
	CTTCATAGTCTCGAGTTTCT+TGG	-	chr7.4:22260177-22260196	None:intergenic	40.0%
	GCGAAGGTTCTTGTAAGAAT+TGG	-	chr7.4:22260135-22260154	None:intergenic	40.0%
	GGTTCTTGTAAGAATTGGTG+CGG	-	chr7.4:22260130-22260149	None:intergenic	40.0%
	TTATGAAGATGAGCCATCGA+GGG	+	chr7.4:22260411-22260430	MS.gene51910:CDS	40.0%
!	CAGGAAGTATAGAAATGCCA+TGG	-	chr7.4:22260465-22260484	None:intergenic	40.0%
!	CTCTTCAAGTTTAAATCCGG+TGG	+	chr7.4:22260290-22260309	MS.gene51910:CDS	40.0%
!	CTGGTTCTCTTTGTGGTATT+TGG	+	chr7.4:22260323-22260342	MS.gene51910:CDS	40.0%
!	TCAGATTTCAAGTACTCGGA+TGG	+	chr7.4:22260035-22260054	MS.gene51910:CDS	40.0%
	AAGAGATACCCACAACAGTG+AGG	-	chr7.4:22260061-22260080	None:intergenic	45.0%
	ACAAGCCCAAAAGGCTTGAA+TGG	-	chr7.4:22260392-22260411	None:intergenic	45.0%
	CAAGTTTAAATCCGGTGGTG+TGG	+	chr7.4:22260295-22260314	MS.gene51910:CDS	45.0%
	CTGTACAGCTATTGTCTGTG+AGG	+	chr7.4:22260082-22260101	MS.gene51910:CDS	45.0%
	GTTATGAAGATGAGCCATCG+AGG	+	chr7.4:22260410-22260429	MS.gene51910:CDS	45.0%
!	CTTGGAAGCTTTGTCGATAG+AGG	-	chr7.4:22260159-22260178	None:intergenic	45.0%
!	TGATCCCACTGATTGTTCCA+TGG	+	chr7.4:22260445-22260464	MS.gene51910:CDS	45.0%
!!	CTTTGAGGCTAGTCTCAACA+CGG	-	chr7.4:22260250-22260269	None:intergenic	45.0%
	CATTACCTTGCAACCCTCGA+TGG	-	chr7.4:22260427-22260446	None:intergenic	50.0%
	CTTTGTCGATAGAGGAGCGA+AGG	-	chr7.4:22260151-22260170	None:intergenic	50.0%
	TGTCTGTGAGGCCATCTCAT+GGG	+	chr7.4:22260094-22260113	MS.gene51910:CDS	50.0%
	TTGTCTGTGAGGCCATCTCA+TGG	+	chr7.4:22260093-22260112	MS.gene51910:CDS	50.0%
!!	GGTTGCTCTGGTTCTCTTTG+TGG	+	chr7.4:22260316-22260335	MS.gene51910:CDS	50.0%
	AACCAGAGCAACCACACCAC+CGG	-	chr7.4:22260309-22260328	None:intergenic	55.0%
	ATGAGCCATCGAGGGTTGCA+AGG	+	chr7.4:22260419-22260438	MS.gene51910:CDS	55.0%
	TCGGATGGCCTCACTGTTGT+GGG	+	chr7.4:22260050-22260069	MS.gene51910:CDS	55.0%
!	CTGGTCTTTGGGTCTGGCAT+TGG	-	chr7.4:22260566-22260585	None:intergenic	55.0%
!	CTTGGGTTTTGCACCGCCAA+GGG	+	chr7.4:22260520-22260539	MS.gene51910:CDS	55.0%
!	TCTTGGGTTTTGCACCGCCA+AGG	+	chr7.4:22260519-22260538	MS.gene51910:CDS	55.0%
!	TTGGGTTTTGCACCGCCAAG+GGG	+	chr7.4:22260521-22260540	MS.gene51910:CDS	55.0%
	ATCCGGTGGTGTGGTTGCTC+TGG	+	chr7.4:22260304-22260323	MS.gene51910:CDS	60.0%
	CTCGGATGGCCTCACTGTTG+TGG	+	chr7.4:22260049-22260068	MS.gene51910:CDS	60.0%
	AGACCAGCACCTGCACCCCT+TGG	-	chr7.4:22260539-22260558	None:intergenic	65.0%
!	TTTGCACCGCCAAGGGGTGC+AGG	+	chr7.4:22260527-22260546	MS.gene51910:CDS	65.0%
	CCAGCACCTGCACCCCTTGG+CGG	-	chr7.4:22260536-22260555	None:intergenic	70.0%
!	CCGCCAAGGGGTGCAGGTGC+TGG	+	chr7.4:22260533-22260552	MS.gene51910:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	22260020	22260589	22260020	ID=MS.gene51910
chr7.4	mRNA	22260020	22260589	22260020	ID=MS.gene51910.t1;Parent=MS.gene51910
chr7.4	exon	22260020	22260589	22260020	ID=MS.gene51910.t1.exon1;Parent=MS.gene51910.t1
chr7.4	CDS	22260020	22260589	22260020	ID=cds.MS.gene51910.t1;Parent=MS.gene51910.t1

Gene Sequence

>MS.gene51910

ATGGCATATCCCTTCTCAGATTTCAAGTACTCGGATGGCCTCACTGTTGTGGGTATCTCTTTCTGTACAGCTATTGTCTGTGAGGCCATCTCATGGGTTCTTATTTACCGCACCAATTCTTACAAGAACCTTCGCTCCTCTATCGACAAAGCTTCCAAGAAACTCGAGACTATGAAGACAGACAACAACAAAATCAACATTAAGAAGTCGAAGACCAAAAAAATTGACCGTGTTGAGACTAGCCTCAAAGAATCTAGTCGTGACTTGTCTCTCTTCAAGTTTAAATCCGGTGGTGTGGTTGCTCTGGTTCTCTTTGTGGTATTTGGGCTGCTCAATTCGCTATTTGAAGGCAAGGTAGTTGCCAAATTGCCATTCAAGCCTTTTGGGCTTGTTATGAAGATGAGCCATCGAGGGTTGCAAGGTAATGATCCCACTGATTGTTCCATGGCATTTCTATACTTCCTGTGTTCCATTAGTATCAGAACAAATTTGCAGAAGTTCTTGGGTTTTGCACCGCCAAGGGGTGCAGGTGCTGGTCTCTTTCCAATGCCAGACCCAAAGACCAGTTGA

Protein sequence

>MS.gene51910.t1

MAYPFSDFKYSDGLTVVGISFCTAIVCEAISWVLIYRTNSYKNLRSSIDKASKKLETMKTDNNKINIKKSKTKKIDRVETSLKESSRDLSLFKFKSGGVVALVLFVVFGLLNSLFEGKVVAKLPFKPFGLVMKMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS