AlfalfaGEDB | MS.gene067770

M. sativa cultivar XinJiangDaYe / MS.gene067770

KEGG: K21891
GO: GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0006873 GO:0006874 GO:0006875 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0016021 GO:0019725 GO:0022803 GO:0022857 GO:0022890 GO:0030003 GO:0030176 GO:0031224 GO:0031227 GO:0031300 GO:0031301 GO:0032469 GO:0042592 GO:0046873 GO:0048878 GO:0050801 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0072503 GO:0072507 GO:0098771 GO:0110165
PFAM: PF01956.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067770.t1	XP_003624576.1	99.5	189	1	0	1	189	1	189	1.30E-98	369

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067770.t1	Q19714	47.9	163	84	1	12	174	4	165	4.6e-36	152.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067770.t1	B7FGM9	99.5	189	1	0	1	189	1	189	9.4e-99	369.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049807	MS.gene067770	0.838883	2.21E-57	-1.69E-46
MS.gene051234	MS.gene067770	0.805837	1.10E-49	-1.69E-46
MS.gene052155	MS.gene067770	-0.80633	8.67E-50	-1.69E-46
MS.gene053144	MS.gene067770	0.802334	5.89E-49	-1.69E-46
MS.gene053536	MS.gene067770	0.80713	5.87E-50	-1.69E-46
MS.gene053538	MS.gene067770	0.814591	1.42E-51	-1.69E-46
MS.gene057102	MS.gene067770	0.816378	5.70E-52	-1.69E-46
MS.gene059424	MS.gene067770	0.812057	5.13E-51	-1.69E-46
MS.gene059900	MS.gene067770	-0.840559	8.09E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene41689	MS.gene067770	PPI
MS.gene067770	MS.gene51947	PPI
MS.gene067770	MS.gene51910	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067770.t1	MTR_7g085050	99.471	189	1	0	1	189	1	189	1.55e-137	382

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067770.t1	AT5G06660	83.069	189	28	2	5	189	8	196	4.05e-102	292
MS.gene067770.t1	AT3G12030	83.069	189	28	2	5	189	8	196	7.39e-95	274

Find 54 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGGTTCTCTTTGTGGTATT+TGG	0.104469	7.1:+19934035	MS.gene067770:CDS
AAAAGGCTTGAATGGCAATT+TGG	0.263436	7.1:-19934093	None:intergenic
AAATTGCCATTCAAGCCTTT+TGG	0.268584	7.1:+19934095	MS.gene067770:CDS
TGGTTCTCTTTGTGGTATTT+GGG	0.271042	7.1:+19934036	MS.gene067770:CDS
AACAAATTTGCAGAAGTTCT+TGG	0.285687	7.1:+19934214	MS.gene067770:CDS
GAACTAGATCAACTGGTCTT+TGG	0.297367	7.1:-19934287	None:intergenic
CTTCATAGTCTCGAGTTTCT+TGG	0.298943	7.1:-19933886	None:intergenic
AACTAGATCAACTGGTCTTT+GGG	0.342623	7.1:-19934286	None:intergenic
CAAATTTGTTCTGATACTAA+TGG	0.343691	7.1:-19934201	None:intergenic
GGTTGCTCTGGTTCTCTTTG+TGG	0.348482	7.1:+19934028	MS.gene067770:CDS
CCGCCAAGGGGTGCAGGTGC+TGG	0.379709	7.1:+19934245	MS.gene067770:CDS
ACAAATTTGCAGAAGTTCTT+GGG	0.395345	7.1:+19934215	MS.gene067770:CDS
CTGGTCTTTGGGTCTGGCAT+TGG	0.395377	7.1:-19934275	None:intergenic
GCGAAGGTTCTTGTAAGAAT+TGG	0.409536	7.1:-19933844	None:intergenic
CATAACAGAACTAGATCAAC+TGG	0.415941	7.1:-19934294	None:intergenic
ATCCGGTGGTGTGGTTGCTC+TGG	0.434250	7.1:+19934016	MS.gene067770:CDS
TTTGCACCGCCAAGGGGTGC+AGG	0.441884	7.1:+19934239	MS.gene067770:CDS
TCTCTCTTCAAGTTTAAATC+CGG	0.442713	7.1:+19933999	MS.gene067770:CDS
CTTGGAAGCTTTGTCGATAG+AGG	0.450316	7.1:-19933868	None:intergenic
CTCGGATGGCCTCACTGTTG+TGG	0.453737	7.1:+19933761	MS.gene067770:CDS
CTGCTCAATTCGCTATTTGA+AGG	0.455366	7.1:+19934059	MS.gene067770:CDS
GATCAACTGGTCTTTGGGTC+TGG	0.458148	7.1:-19934281	None:intergenic
CCAGCACCTGCACCCCTTGG+CGG	0.467860	7.1:-19934245	None:intergenic
TCGGATGGCCTCACTGTTGT+GGG	0.468742	7.1:+19933762	MS.gene067770:CDS
CTTCTCAGATTTCAAGTACT+CGG	0.477023	7.1:+19933743	MS.gene067770:CDS
ACAAGCCCAAAAGGCTTGAA+TGG	0.484472	7.1:-19934101	None:intergenic
ATCTTCATAACAAGCCCAAA+AGG	0.485077	7.1:-19934110	None:intergenic
TTGTCTGTGAGGCCATCTCA+TGG	0.486509	7.1:+19933805	MS.gene067770:CDS
CTCTTCAAGTTTAAATCCGG+TGG	0.507122	7.1:+19934002	MS.gene067770:CDS
AGACCAGCACCTGCACCCCT+TGG	0.507589	7.1:-19934248	None:intergenic
CGAGTACTTGAAATCTGAGA+AGG	0.540066	7.1:-19933742	None:intergenic
AGTCACGACTAGATTCTTTG+AGG	0.544166	7.1:-19933974	None:intergenic
CAATTCGCTATTTGAAGGCA+AGG	0.549223	7.1:+19934064	MS.gene067770:CDS
TGTCTGTGAGGCCATCTCAT+GGG	0.550860	7.1:+19933806	MS.gene067770:CDS
CAAGTTTAAATCCGGTGGTG+TGG	0.561902	7.1:+19934007	MS.gene067770:CDS
AACCAGAGCAACCACACCAC+CGG	0.570086	7.1:-19934018	None:intergenic
TCAGATTTCAAGTACTCGGA+TGG	0.574798	7.1:+19933747	MS.gene067770:CDS
AAATGCCATGGAACAATCAG+TGG	0.579830	7.1:-19934162	None:intergenic
ATGAGCCATCGAGGGTTGCA+AGG	0.585212	7.1:+19934131	MS.gene067770:CDS
CTTTGTCGATAGAGGAGCGA+AGG	0.585269	7.1:-19933860	None:intergenic
CATTACCTTGCAACCCTCGA+TGG	0.592832	7.1:-19934136	None:intergenic
TGATCCCACTGATTGTTCCA+TGG	0.598157	7.1:+19934157	MS.gene067770:CDS
GTAAATAAGAACCCATGAGA+TGG	0.599701	7.1:-19933817	None:intergenic
TTCTGATACTAATGGAACAC+AGG	0.602611	7.1:-19934193	None:intergenic
CTGTACAGCTATTGTCTGTG+AGG	0.606267	7.1:+19933794	MS.gene067770:CDS
ATCTGAGAAGGGATATGCCA+TGG	0.607371	7.1:-19933730	None:intergenic
CAGGAAGTATAGAAATGCCA+TGG	0.612214	7.1:-19934174	None:intergenic
TTATGAAGATGAGCCATCGA+GGG	0.622078	7.1:+19934123	MS.gene067770:CDS
GAGTACTTGAAATCTGAGAA+GGG	0.629348	7.1:-19933741	None:intergenic
GGTTCTTGTAAGAATTGGTG+CGG	0.654185	7.1:-19933839	None:intergenic
CTTTGAGGCTAGTCTCAACA+CGG	0.657831	7.1:-19933959	None:intergenic
AATGCCATGGAACAATCAGT+GGG	0.668389	7.1:-19934161	None:intergenic
GTTATGAAGATGAGCCATCG+AGG	0.685544	7.1:+19934122	MS.gene067770:CDS
AAGAGATACCCACAACAGTG+AGG	0.718334	7.1:-19933770	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAATTTGTTCTGATACTAA+TGG	-	chr7.1:19934204-19934223	None:intergenic	25.0%
	AACAAATTTGCAGAAGTTCT+TGG	+	chr7.1:19934214-19934233	MS.gene067770:CDS	30.0%
	ACAAATTTGCAGAAGTTCTT+GGG	+	chr7.1:19934215-19934234	MS.gene067770:CDS	30.0%
!	TCTCTCTTCAAGTTTAAATC+CGG	+	chr7.1:19933999-19934018	MS.gene067770:CDS	30.0%
	AAAAGGCTTGAATGGCAATT+TGG	-	chr7.1:19934096-19934115	None:intergenic	35.0%
	ATCTTCATAACAAGCCCAAA+AGG	-	chr7.1:19934113-19934132	None:intergenic	35.0%
	GAGTACTTGAAATCTGAGAA+GGG	-	chr7.1:19933744-19933763	None:intergenic	35.0%
	GTAAATAAGAACCCATGAGA+TGG	-	chr7.1:19933820-19933839	None:intergenic	35.0%
	TGGTTCTCTTTGTGGTATTT+GGG	+	chr7.1:19934036-19934055	MS.gene067770:CDS	35.0%
	TTCTGATACTAATGGAACAC+AGG	-	chr7.1:19934196-19934215	None:intergenic	35.0%
!	AAATTGCCATTCAAGCCTTT+TGG	+	chr7.1:19934095-19934114	MS.gene067770:CDS	35.0%
!	AATTGCCATTCAAGCCTTTT+GGG	+	chr7.1:19934096-19934115	MS.gene067770:CDS	35.0%
!	CTTCTCAGATTTCAAGTACT+CGG	+	chr7.1:19933743-19933762	MS.gene067770:CDS	35.0%
!!!	CTCAACACGGTCAATTTTTT+TGG	-	chr7.1:19933949-19933968	None:intergenic	35.0%
	AAATGCCATGGAACAATCAG+TGG	-	chr7.1:19934165-19934184	None:intergenic	40.0%
	AATGCCATGGAACAATCAGT+GGG	-	chr7.1:19934164-19934183	None:intergenic	40.0%
	AGTCACGACTAGATTCTTTG+AGG	-	chr7.1:19933977-19933996	None:intergenic	40.0%
	CAATTCGCTATTTGAAGGCA+AGG	+	chr7.1:19934064-19934083	MS.gene067770:CDS	40.0%
	CGAGTACTTGAAATCTGAGA+AGG	-	chr7.1:19933745-19933764	None:intergenic	40.0%
	CTGCTCAATTCGCTATTTGA+AGG	+	chr7.1:19934059-19934078	MS.gene067770:CDS	40.0%
	CTTCATAGTCTCGAGTTTCT+TGG	-	chr7.1:19933889-19933908	None:intergenic	40.0%
	GCGAAGGTTCTTGTAAGAAT+TGG	-	chr7.1:19933847-19933866	None:intergenic	40.0%
	GGTTCTTGTAAGAATTGGTG+CGG	-	chr7.1:19933842-19933861	None:intergenic	40.0%
	TTATGAAGATGAGCCATCGA+GGG	+	chr7.1:19934123-19934142	MS.gene067770:CDS	40.0%
!	CAGGAAGTATAGAAATGCCA+TGG	-	chr7.1:19934177-19934196	None:intergenic	40.0%
!	CTCTTCAAGTTTAAATCCGG+TGG	+	chr7.1:19934002-19934021	MS.gene067770:CDS	40.0%
!	CTGGTTCTCTTTGTGGTATT+TGG	+	chr7.1:19934035-19934054	MS.gene067770:CDS	40.0%
!	TCAGATTTCAAGTACTCGGA+TGG	+	chr7.1:19933747-19933766	MS.gene067770:CDS	40.0%
	AAGAGATACCCACAACAGTG+AGG	-	chr7.1:19933773-19933792	None:intergenic	45.0%
	ACAAGCCCAAAAGGCTTGAA+TGG	-	chr7.1:19934104-19934123	None:intergenic	45.0%
	CAAGTTTAAATCCGGTGGTG+TGG	+	chr7.1:19934007-19934026	MS.gene067770:CDS	45.0%
	CTGTACAGCTATTGTCTGTG+AGG	+	chr7.1:19933794-19933813	MS.gene067770:CDS	45.0%
	GTTATGAAGATGAGCCATCG+AGG	+	chr7.1:19934122-19934141	MS.gene067770:CDS	45.0%
!	CTTGGAAGCTTTGTCGATAG+AGG	-	chr7.1:19933871-19933890	None:intergenic	45.0%
!	TGATCCCACTGATTGTTCCA+TGG	+	chr7.1:19934157-19934176	MS.gene067770:CDS	45.0%
!!	CTTTGAGGCTAGTCTCAACA+CGG	-	chr7.1:19933962-19933981	None:intergenic	45.0%
	CATTACCTTGCAACCCTCGA+TGG	-	chr7.1:19934139-19934158	None:intergenic	50.0%
	CTTTGTCGATAGAGGAGCGA+AGG	-	chr7.1:19933863-19933882	None:intergenic	50.0%
	TGTCTGTGAGGCCATCTCAT+GGG	+	chr7.1:19933806-19933825	MS.gene067770:CDS	50.0%
	TTGTCTGTGAGGCCATCTCA+TGG	+	chr7.1:19933805-19933824	MS.gene067770:CDS	50.0%
!!	GGTTGCTCTGGTTCTCTTTG+TGG	+	chr7.1:19934028-19934047	MS.gene067770:CDS	50.0%
	AACCAGAGCAACCACACCAC+CGG	-	chr7.1:19934021-19934040	None:intergenic	55.0%
	ATGAGCCATCGAGGGTTGCA+AGG	+	chr7.1:19934131-19934150	MS.gene067770:CDS	55.0%
	TCGGATGGCCTCACTGTTGT+GGG	+	chr7.1:19933762-19933781	MS.gene067770:CDS	55.0%
!	CTGGTCTTTGGGTCTGGCAT+TGG	-	chr7.1:19934278-19934297	None:intergenic	55.0%
!	CTTGGGTTTTGCACCGCCAA+GGG	+	chr7.1:19934232-19934251	MS.gene067770:CDS	55.0%
!	TCTTGGGTTTTGCACCGCCA+AGG	+	chr7.1:19934231-19934250	MS.gene067770:CDS	55.0%
!	TTGGGTTTTGCACCGCCAAG+GGG	+	chr7.1:19934233-19934252	MS.gene067770:CDS	55.0%
	ATCCGGTGGTGTGGTTGCTC+TGG	+	chr7.1:19934016-19934035	MS.gene067770:CDS	60.0%
	CTCGGATGGCCTCACTGTTG+TGG	+	chr7.1:19933761-19933780	MS.gene067770:CDS	60.0%
	AGACCAGCACCTGCACCCCT+TGG	-	chr7.1:19934251-19934270	None:intergenic	65.0%
!	TTTGCACCGCCAAGGGGTGC+AGG	+	chr7.1:19934239-19934258	MS.gene067770:CDS	65.0%
	CCAGCACCTGCACCCCTTGG+CGG	-	chr7.1:19934248-19934267	None:intergenic	70.0%
!	CCGCCAAGGGGTGCAGGTGC+TGG	+	chr7.1:19934245-19934264	MS.gene067770:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	19933732	19934301	19933732	ID=MS.gene067770
chr7.1	mRNA	19933732	19934301	19933732	ID=MS.gene067770.t1;Parent=MS.gene067770
chr7.1	exon	19933732	19934301	19933732	ID=MS.gene067770.t1.exon1;Parent=MS.gene067770.t1
chr7.1	CDS	19933732	19934301	19933732	ID=cds.MS.gene067770.t1;Parent=MS.gene067770.t1

Gene Sequence

>MS.gene067770

ATGGCATATCCCTTCTCAGATTTCAAGTACTCGGATGGCCTCACTGTTGTGGGTATCTCTTTCTGTACAGCTATTGTCTGTGAGGCCATCTCATGGGTTCTTATTTACCGCACCAATTCTTACAAGAACCTTCGCTCCTCTATCGACAAAGCTTCCAAGAAACTCGAGACTATGAAGACAGACAACAACAAAATCAACATTAAGAAGTCGAAGACCAAAAAAATTGACCGTGTTGAGACTAGCCTCAAAGAATCTAGTCGTGACTTGTCTCTCTTCAAGTTTAAATCCGGTGGTGTGGTTGCTCTGGTTCTCTTTGTGGTATTTGGGCTGCTCAATTCGCTATTTGAAGGCAAGGTAGTTGCCAAATTGCCATTCAAGCCTTTTGGGCTTGTTATGAAGATGAGCCATCGAGGGTTGCAAGGTAATGATCCCACTGATTGTTCCATGGCATTTCTATACTTCCTGTGTTCCATTAGTATCAGAACAAATTTGCAGAAGTTCTTGGGTTTTGCACCGCCAAGGGGTGCAGGTGCTGGTCTCTTTCCAATGCCAGACCCAAAGACCAGTTGA

Protein sequence

>MS.gene067770.t1

MAYPFSDFKYSDGLTVVGISFCTAIVCEAISWVLIYRTNSYKNLRSSIDKASKKLETMKTDNNKINIKKSKTKKIDRVETSLKESSRDLSLFKFKSGGVVALVLFVVFGLLNSLFEGKVVAKLPFKPFGLVMKMSHRGLQGNDPTDCSMAFLYFLCSISIRTNLQKFLGFAPPRGAGAGLFPMPDPKTS