Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42572.t1 | GAU17947.1 | 65.3 | 426 | 138 | 5 | 1 | 416 | 1 | 426 | 1.00E-140 | 510 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42572.t1 | Q9FZ70 | 27.2 | 427 | 268 | 13 | 14 | 415 | 6 | 414 | 5.6e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42572.t1 | G7KSK2 | 60.3 | 428 | 151 | 9 | 1 | 415 | 1 | 422 | 9.3e-123 | 449.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene42572 | -0.808936 | 2.42E-50 | -1.69E-46 |
| MS.gene04917 | MS.gene42572 | -0.800109 | 1.68E-48 | -1.69E-46 |
| MS.gene04991 | MS.gene42572 | 0.836094 | 1.14E-56 | -1.69E-46 |
| MS.gene050114 | MS.gene42572 | -0.818548 | 1.85E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene42572 | 0.837104 | 6.33E-57 | -1.69E-46 |
| MS.gene050762 | MS.gene42572 | -0.825948 | 3.55E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene42572 | -0.826161 | 3.16E-54 | -1.69E-46 |
| MS.gene056464 | MS.gene42572 | 0.817486 | 3.22E-52 | -1.69E-46 |
| MS.gene056849 | MS.gene42572 | -0.81101 | 8.67E-51 | -1.69E-46 |
| MS.gene060084 | MS.gene42572 | -0.857874 | 1.21E-62 | -1.69E-46 |
| MS.gene060869 | MS.gene42572 | -0.818776 | 1.64E-52 | -1.69E-46 |
| MS.gene060870 | MS.gene42572 | -0.814389 | 1.58E-51 | -1.69E-46 |
| MS.gene060940 | MS.gene42572 | -0.809959 | 1.46E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42572.t1 | MTR_7g090750 | 60.981 | 428 | 148 | 9 | 1 | 415 | 1 | 422 | 9.51e-157 | 449 |
| MS.gene42572.t1 | MTR_5g038430 | 50.816 | 429 | 184 | 10 | 1 | 416 | 82 | 496 | 4.70e-118 | 353 |
| MS.gene42572.t1 | MTR_0227s0010 | 52.899 | 276 | 100 | 6 | 1 | 269 | 1 | 253 | 1.13e-85 | 265 |
| MS.gene42572.t1 | MTR_0227s0010 | 42.202 | 109 | 60 | 1 | 305 | 410 | 230 | 338 | 1.45e-12 | 68.9 |
| MS.gene42572.t1 | MTR_8g095590 | 45.732 | 328 | 131 | 13 | 1 | 318 | 1 | 291 | 4.47e-63 | 211 |
| MS.gene42572.t1 | MTR_7g090760 | 55.556 | 162 | 50 | 2 | 85 | 245 | 1 | 141 | 5.03e-52 | 173 |
| MS.gene42572.t1 | MTR_8g028505 | 51.613 | 217 | 71 | 7 | 137 | 347 | 1 | 189 | 5.25e-52 | 174 |
| MS.gene42572.t1 | MTR_0049s0110 | 30.233 | 430 | 255 | 14 | 14 | 416 | 8 | 419 | 3.36e-39 | 145 |
| MS.gene42572.t1 | MTR_4g057725 | 29.032 | 434 | 256 | 13 | 13 | 412 | 21 | 436 | 1.08e-32 | 128 |
| MS.gene42572.t1 | MTR_2g091145 | 29.630 | 405 | 245 | 14 | 4 | 377 | 11 | 406 | 3.82e-32 | 127 |
| MS.gene42572.t1 | MTR_1g021825 | 30.626 | 431 | 248 | 15 | 14 | 415 | 17 | 425 | 6.78e-32 | 125 |
| MS.gene42572.t1 | MTR_2g047858 | 29.099 | 433 | 254 | 12 | 14 | 412 | 115 | 528 | 9.62e-32 | 127 |
| MS.gene42572.t1 | MTR_1g021825 | 30.626 | 431 | 248 | 15 | 14 | 415 | 88 | 496 | 1.42e-31 | 126 |
| MS.gene42572.t1 | MTR_2g047848 | 28.375 | 437 | 253 | 14 | 14 | 412 | 7 | 421 | 1.78e-31 | 124 |
| MS.gene42572.t1 | MTR_2g047872 | 30.233 | 430 | 250 | 12 | 14 | 412 | 23 | 433 | 7.97e-31 | 123 |
| MS.gene42572.t1 | MTR_0049s0110 | 37.131 | 237 | 131 | 8 | 14 | 244 | 8 | 232 | 9.21e-31 | 119 |
| MS.gene42572.t1 | MTR_0049s0110 | 31.077 | 325 | 190 | 11 | 14 | 318 | 8 | 318 | 4.10e-30 | 119 |
| MS.gene42572.t1 | MTR_0049s0110 | 31.077 | 325 | 190 | 11 | 14 | 318 | 8 | 318 | 5.57e-30 | 119 |
| MS.gene42572.t1 | MTR_1g013840 | 29.535 | 430 | 256 | 13 | 14 | 412 | 56 | 469 | 8.31e-29 | 117 |
| MS.gene42572.t1 | MTR_1g011410 | 33.131 | 329 | 190 | 10 | 14 | 321 | 315 | 634 | 1.13e-28 | 119 |
| MS.gene42572.t1 | MTR_1g011410 | 30.178 | 338 | 161 | 9 | 14 | 322 | 7 | 298 | 6.14e-26 | 110 |
| MS.gene42572.t1 | MTR_1g011400 | 31.395 | 430 | 254 | 14 | 14 | 415 | 7 | 423 | 1.16e-27 | 114 |
| MS.gene42572.t1 | MTR_1g011430 | 39.806 | 206 | 118 | 4 | 14 | 218 | 7 | 207 | 4.91e-27 | 111 |
| MS.gene42572.t1 | MTR_1g016340 | 28.605 | 430 | 265 | 11 | 14 | 412 | 15 | 433 | 7.86e-27 | 112 |
| MS.gene42572.t1 | MTR_1g016340 | 28.605 | 430 | 265 | 11 | 14 | 412 | 20 | 438 | 7.88e-27 | 112 |
| MS.gene42572.t1 | MTR_1g013560 | 27.002 | 437 | 267 | 11 | 13 | 415 | 18 | 436 | 1.10e-26 | 111 |
| MS.gene42572.t1 | MTR_1g013580 | 29.195 | 435 | 255 | 15 | 12 | 414 | 17 | 430 | 4.98e-26 | 109 |
| MS.gene42572.t1 | MTR_1g021820 | 28.337 | 427 | 269 | 12 | 8 | 415 | 22 | 430 | 8.87e-26 | 108 |
| MS.gene42572.t1 | MTR_1g014450 | 27.674 | 430 | 261 | 14 | 14 | 412 | 16 | 426 | 1.51e-24 | 105 |
| MS.gene42572.t1 | MTR_1g013560 | 28.228 | 333 | 209 | 8 | 13 | 321 | 18 | 344 | 1.44e-23 | 101 |
| MS.gene42572.t1 | MTR_1g013630 | 31.879 | 298 | 165 | 12 | 14 | 298 | 39 | 311 | 1.60e-23 | 101 |
| MS.gene42572.t1 | MTR_1g016310 | 35.885 | 209 | 126 | 6 | 14 | 216 | 20 | 226 | 7.01e-23 | 99.0 |
| MS.gene42572.t1 | MTR_1g016310 | 35.885 | 209 | 126 | 6 | 14 | 216 | 20 | 226 | 2.17e-22 | 99.0 |
| MS.gene42572.t1 | MTR_5g040850 | 28.205 | 429 | 257 | 13 | 14 | 412 | 7 | 414 | 5.05e-22 | 97.8 |
| MS.gene42572.t1 | MTR_1g013610 | 29.755 | 326 | 192 | 11 | 12 | 319 | 5 | 311 | 4.78e-20 | 91.7 |
| MS.gene42572.t1 | MTR_1g028860 | 31.863 | 204 | 126 | 5 | 27 | 221 | 3 | 202 | 4.29e-15 | 75.5 |
| MS.gene42572.t1 | MTR_2g007500 | 30.102 | 196 | 111 | 6 | 13 | 201 | 13 | 189 | 2.79e-14 | 73.9 |
| MS.gene42572.t1 | MTR_2g007450 | 25.436 | 401 | 250 | 20 | 8 | 378 | 10 | 391 | 5.30e-14 | 73.6 |
| MS.gene42572.t1 | MTR_4g080590 | 31.500 | 200 | 117 | 7 | 11 | 202 | 20 | 207 | 1.47e-13 | 72.0 |
| MS.gene42572.t1 | MTR_4g080590 | 31.500 | 200 | 117 | 7 | 11 | 202 | 20 | 207 | 1.85e-13 | 72.0 |
| MS.gene42572.t1 | MTR_7g075820 | 26.010 | 396 | 224 | 15 | 14 | 377 | 6 | 364 | 2.97e-13 | 71.2 |
| MS.gene42572.t1 | MTR_3g107160 | 26.316 | 418 | 238 | 19 | 11 | 407 | 7 | 375 | 6.32e-13 | 70.1 |
| MS.gene42572.t1 | MTR_8g085430 | 32.178 | 202 | 115 | 10 | 13 | 201 | 10 | 202 | 1.46e-12 | 68.6 |
| MS.gene42572.t1 | MTR_8g014290 | 29.932 | 147 | 90 | 5 | 14 | 147 | 10 | 156 | 2.64e-12 | 65.1 |
| MS.gene42572.t1 | MTR_3g048290 | 31.841 | 201 | 121 | 7 | 11 | 202 | 20 | 213 | 3.16e-12 | 68.6 |
| MS.gene42572.t1 | MTR_7g105510 | 30.045 | 223 | 128 | 7 | 17 | 215 | 35 | 253 | 6.55e-12 | 66.6 |
| MS.gene42572.t1 | MTR_7g056433 | 28.431 | 204 | 133 | 5 | 3 | 200 | 1 | 197 | 9.05e-12 | 66.6 |
| MS.gene42572.t1 | MTR_7g098570 | 26.110 | 383 | 234 | 14 | 7 | 377 | 13 | 358 | 2.26e-11 | 65.9 |
| MS.gene42572.t1 | MTR_7g088260 | 30.040 | 253 | 157 | 10 | 14 | 260 | 91 | 329 | 3.48e-11 | 65.1 |
| MS.gene42572.t1 | MTR_7g056447 | 33.690 | 187 | 111 | 8 | 14 | 196 | 9 | 186 | 3.86e-11 | 63.9 |
| MS.gene42572.t1 | MTR_2g100590 | 26.271 | 236 | 145 | 8 | 11 | 228 | 39 | 263 | 5.30e-11 | 64.3 |
| MS.gene42572.t1 | MTR_2g100590 | 26.271 | 236 | 145 | 8 | 11 | 228 | 39 | 263 | 6.33e-11 | 64.3 |
| MS.gene42572.t1 | MTR_7g023310 | 29.187 | 209 | 122 | 7 | 5 | 202 | 17 | 210 | 6.53e-11 | 64.3 |
| MS.gene42572.t1 | MTR_2g100590 | 26.271 | 236 | 145 | 8 | 11 | 228 | 39 | 263 | 6.66e-11 | 64.7 |
| MS.gene42572.t1 | MTR_8g062270 | 28.000 | 250 | 135 | 10 | 11 | 221 | 13 | 256 | 6.84e-11 | 64.3 |
| MS.gene42572.t1 | MTR_3g026920 | 24.272 | 412 | 227 | 16 | 8 | 378 | 13 | 380 | 7.23e-11 | 63.9 |
| MS.gene42572.t1 | MTR_7g094030 | 28.182 | 220 | 130 | 7 | 14 | 218 | 28 | 234 | 8.31e-11 | 63.9 |
| MS.gene42572.t1 | MTR_2g007400 | 26.794 | 209 | 141 | 6 | 3 | 199 | 1 | 209 | 9.40e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42572.t1 | AT1G13570 | 30.607 | 428 | 254 | 13 | 14 | 416 | 6 | 415 | 2.66e-33 | 129 |
| MS.gene42572.t1 | AT1G13570 | 30.607 | 428 | 254 | 13 | 14 | 416 | 6 | 415 | 2.66e-33 | 129 |
| MS.gene42572.t1 | AT1G13570 | 35.021 | 237 | 135 | 6 | 14 | 244 | 6 | 229 | 1.12e-27 | 111 |
| MS.gene42572.t1 | AT3G50710 | 26.535 | 456 | 247 | 23 | 13 | 412 | 1 | 424 | 7.89e-22 | 97.4 |
| MS.gene42572.t1 | AT1G69630 | 25.383 | 457 | 279 | 15 | 6 | 415 | 10 | 451 | 1.35e-17 | 84.7 |
| MS.gene42572.t1 | AT2G04230 | 27.040 | 429 | 238 | 23 | 14 | 389 | 13 | 419 | 7.28e-16 | 79.7 |
| MS.gene42572.t1 | AT3G26922 | 30.078 | 256 | 140 | 9 | 14 | 239 | 14 | 260 | 1.20e-15 | 77.8 |
| MS.gene42572.t1 | AT3G26922 | 30.078 | 256 | 140 | 9 | 14 | 239 | 14 | 260 | 1.20e-15 | 77.8 |
| MS.gene42572.t1 | AT5G44950 | 26.638 | 458 | 257 | 21 | 14 | 415 | 4 | 438 | 1.40e-15 | 78.6 |
| MS.gene42572.t1 | AT1G66290 | 29.146 | 199 | 126 | 7 | 4 | 199 | 19 | 205 | 7.80e-15 | 76.6 |
| MS.gene42572.t1 | AT1G51370 | 32.292 | 192 | 116 | 7 | 7 | 197 | 13 | 191 | 1.12e-14 | 75.1 |
| MS.gene42572.t1 | AT1G51370 | 32.292 | 192 | 116 | 7 | 7 | 197 | 13 | 191 | 1.41e-14 | 75.5 |
| MS.gene42572.t1 | AT1G51370 | 32.292 | 192 | 116 | 7 | 7 | 197 | 13 | 191 | 1.54e-14 | 75.5 |
| MS.gene42572.t1 | AT4G14103 | 32.099 | 243 | 144 | 10 | 14 | 243 | 8 | 242 | 2.72e-14 | 74.7 |
| MS.gene42572.t1 | AT4G14103 | 32.099 | 243 | 144 | 10 | 14 | 243 | 8 | 242 | 2.74e-14 | 74.3 |
| MS.gene42572.t1 | AT4G14103 | 32.099 | 243 | 144 | 10 | 14 | 243 | 8 | 242 | 2.74e-14 | 74.3 |
| MS.gene42572.t1 | AT1G78750 | 28.500 | 200 | 137 | 5 | 6 | 200 | 10 | 208 | 2.75e-14 | 74.7 |
| MS.gene42572.t1 | AT1G78750 | 28.500 | 200 | 137 | 5 | 6 | 200 | 10 | 208 | 2.75e-14 | 74.7 |
| MS.gene42572.t1 | AT4G13985 | 26.866 | 201 | 138 | 6 | 7 | 200 | 12 | 210 | 7.35e-14 | 73.6 |
| MS.gene42572.t1 | AT3G58860 | 30.928 | 194 | 123 | 5 | 11 | 197 | 4 | 193 | 2.13e-13 | 72.0 |
| MS.gene42572.t1 | AT1G78840 | 28.736 | 261 | 147 | 12 | 19 | 263 | 11 | 248 | 3.22e-13 | 71.6 |
| MS.gene42572.t1 | AT1G78840 | 28.736 | 261 | 147 | 12 | 19 | 263 | 11 | 248 | 3.22e-13 | 71.6 |
| MS.gene42572.t1 | AT5G56690 | 27.650 | 217 | 137 | 9 | 16 | 220 | 4 | 212 | 4.66e-13 | 70.9 |
| MS.gene42572.t1 | AT5G53635 | 31.100 | 209 | 123 | 10 | 16 | 218 | 2 | 195 | 5.64e-13 | 70.5 |
| MS.gene42572.t1 | AT1G66300 | 30.000 | 200 | 125 | 7 | 4 | 199 | 20 | 208 | 7.38e-13 | 70.5 |
| MS.gene42572.t1 | AT1G80960 | 29.756 | 205 | 125 | 8 | 12 | 203 | 9 | 207 | 1.11e-12 | 69.7 |
| MS.gene42572.t1 | AT5G56440 | 28.796 | 191 | 121 | 7 | 13 | 197 | 1 | 182 | 1.27e-12 | 69.7 |
| MS.gene42572.t1 | AT1G80960 | 29.756 | 205 | 125 | 8 | 12 | 203 | 48 | 246 | 1.58e-12 | 68.9 |
| MS.gene42572.t1 | AT1G80960 | 29.756 | 205 | 125 | 8 | 12 | 203 | 48 | 246 | 1.82e-12 | 69.3 |
| MS.gene42572.t1 | AT1G80960 | 29.756 | 205 | 125 | 8 | 12 | 203 | 48 | 246 | 1.82e-12 | 69.3 |
| MS.gene42572.t1 | AT1G80960 | 29.756 | 205 | 125 | 8 | 12 | 203 | 48 | 246 | 1.82e-12 | 69.3 |
| MS.gene42572.t1 | AT1G80960 | 29.756 | 205 | 125 | 8 | 12 | 203 | 48 | 246 | 1.88e-12 | 69.3 |
| MS.gene42572.t1 | AT5G56420 | 27.778 | 216 | 132 | 7 | 14 | 218 | 6 | 208 | 2.10e-12 | 68.9 |
| MS.gene42572.t1 | AT5G56420 | 27.778 | 216 | 132 | 7 | 14 | 218 | 6 | 208 | 2.10e-12 | 68.9 |
| MS.gene42572.t1 | AT5G56420 | 27.778 | 216 | 132 | 7 | 14 | 218 | 6 | 208 | 2.10e-12 | 68.9 |
| MS.gene42572.t1 | AT1G16930 | 29.798 | 198 | 124 | 7 | 7 | 199 | 8 | 195 | 2.61e-12 | 68.6 |
| MS.gene42572.t1 | AT1G16930 | 29.798 | 198 | 124 | 7 | 7 | 199 | 8 | 195 | 2.61e-12 | 68.6 |
| MS.gene42572.t1 | AT1G16930 | 29.798 | 198 | 124 | 7 | 7 | 199 | 8 | 195 | 2.61e-12 | 68.6 |
| MS.gene42572.t1 | AT5G22610 | 25.785 | 446 | 262 | 21 | 14 | 416 | 18 | 437 | 2.91e-12 | 68.6 |
| MS.gene42572.t1 | AT4G10400 | 29.688 | 192 | 124 | 6 | 13 | 200 | 1 | 185 | 3.74e-12 | 68.2 |
| MS.gene42572.t1 | AT4G10400 | 29.688 | 192 | 124 | 6 | 13 | 200 | 1 | 185 | 3.74e-12 | 68.2 |
| MS.gene42572.t1 | AT4G10400 | 29.688 | 192 | 124 | 6 | 13 | 200 | 1 | 185 | 3.74e-12 | 68.2 |
| MS.gene42572.t1 | AT1G16940 | 26.540 | 211 | 133 | 7 | 1 | 206 | 1 | 194 | 3.81e-12 | 68.2 |
| MS.gene42572.t1 | AT1G66310 | 27.957 | 465 | 245 | 21 | 7 | 415 | 12 | 442 | 1.15e-11 | 66.6 |
| MS.gene42572.t1 | AT1G66320 | 27.451 | 204 | 133 | 7 | 4 | 202 | 19 | 212 | 1.25e-11 | 66.6 |
| MS.gene42572.t1 | AT5G22670 | 29.688 | 192 | 116 | 9 | 14 | 199 | 11 | 189 | 1.49e-11 | 66.2 |
| MS.gene42572.t1 | AT1G60400 | 27.184 | 206 | 134 | 5 | 1 | 199 | 1 | 197 | 1.89e-11 | 65.9 |
| MS.gene42572.t1 | AT4G14096 | 31.280 | 211 | 134 | 7 | 14 | 218 | 8 | 213 | 2.18e-11 | 65.9 |
| MS.gene42572.t1 | AT4G14096 | 31.280 | 211 | 134 | 7 | 14 | 218 | 8 | 213 | 2.18e-11 | 65.9 |
| MS.gene42572.t1 | AT4G00160 | 30.000 | 190 | 119 | 6 | 14 | 197 | 16 | 197 | 2.41e-11 | 65.9 |
| MS.gene42572.t1 | AT4G09920 | 29.630 | 189 | 122 | 6 | 13 | 197 | 1 | 182 | 2.56e-11 | 64.7 |
| MS.gene42572.t1 | AT4G09920 | 29.630 | 189 | 122 | 6 | 13 | 197 | 1 | 182 | 2.56e-11 | 64.7 |
| MS.gene42572.t1 | AT4G09920 | 29.630 | 189 | 122 | 6 | 13 | 197 | 1 | 182 | 2.56e-11 | 64.7 |
| MS.gene42572.t1 | AT1G58310 | 30.189 | 212 | 116 | 8 | 14 | 200 | 8 | 212 | 2.82e-11 | 65.5 |
| MS.gene42572.t1 | AT5G25850 | 29.381 | 194 | 116 | 9 | 13 | 200 | 19 | 197 | 3.06e-11 | 65.5 |
| MS.gene42572.t1 | AT3G58880 | 29.500 | 200 | 129 | 6 | 13 | 204 | 2 | 197 | 3.55e-11 | 65.1 |
| MS.gene42572.t1 | AT4G09920 | 29.630 | 189 | 122 | 6 | 13 | 197 | 1 | 182 | 5.20e-11 | 63.9 |
| MS.gene42572.t1 | AT4G09920 | 29.630 | 189 | 122 | 6 | 13 | 197 | 1 | 182 | 5.20e-11 | 63.9 |
| MS.gene42572.t1 | AT3G26922 | 28.452 | 239 | 132 | 9 | 31 | 239 | 2 | 231 | 6.74e-11 | 63.2 |
| MS.gene42572.t1 | AT3G26922 | 28.452 | 239 | 132 | 9 | 31 | 239 | 2 | 231 | 6.74e-11 | 63.2 |
| MS.gene42572.t1 | AT1G13780 | 28.571 | 203 | 114 | 6 | 14 | 197 | 10 | 200 | 8.52e-11 | 63.9 |
| MS.gene42572.t1 | AT5G22730 | 31.633 | 196 | 110 | 7 | 14 | 199 | 28 | 209 | 8.95e-11 | 63.5 |
Find 60 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTACTTGAGAGTCTTATTTC+TGG | 0.211583 | 7.1:+16517607 | MS.gene42572:CDS |
| ATCCAATGACAGATACTTTA+TGG | 0.217273 | 7.1:-16518258 | None:intergenic |
| TTAATACTCCTCGACAAAAT+AGG | 0.221838 | 7.1:-16517406 | None:intergenic |
| CTTACTTATACTGTTGGTTT+TGG | 0.228920 | 7.1:+16518617 | MS.gene42572:CDS |
| GATCTTAGACTCACAGTATC+TGG | 0.289709 | 7.1:+16517775 | MS.gene42572:CDS |
| TTGACATTCTTTACATTTGC+CGG | 0.321794 | 7.1:+16517499 | MS.gene42572:CDS |
| TCCTCGCTTGGTTGATCTTA+AGG | 0.333872 | 7.1:+16518340 | MS.gene42572:CDS |
| CTCCCACTCTCAAAGTCTTA+TGG | 0.350449 | 7.1:+16517695 | MS.gene42572:CDS |
| GCATCAAATTCAAAATATTC+AGG | 0.356102 | 7.1:-16517673 | None:intergenic |
| TTTCTAACCTTGGTATTGTA+AGG | 0.365718 | 7.1:-16517847 | None:intergenic |
| TAATACTCCTCGACAAAATA+GGG | 0.373617 | 7.1:-16517405 | None:intergenic |
| GCATTTCTGAAGAGTTGTTA+TGG | 0.375426 | 7.1:+16518672 | MS.gene42572:CDS |
| TGTACATCAAAGACCTAGTT+AGG | 0.389200 | 7.1:+16517152 | MS.gene42572:CDS |
| ATACATTGTCTTCTCTTGTA+AGG | 0.390569 | 7.1:+16517474 | MS.gene42572:CDS |
| TTCCAACTCCTCTGAATGCT+CGG | 0.404790 | 7.1:-16518477 | None:intergenic |
| CTTATTGTCTGTTACATTCG+AGG | 0.413736 | 7.1:+16517579 | MS.gene42572:CDS |
| CTCCATAAAGTATCTGTCAT+TGG | 0.434647 | 7.1:+16518256 | MS.gene42572:CDS |
| ATCTTGAAATTGTAGTCATA+AGG | 0.483996 | 7.1:-16517355 | None:intergenic |
| ACTTAAAAGTGCTCCTCGCT+TGG | 0.484927 | 7.1:+16518328 | MS.gene42572:CDS |
| TTACAAGAGAAGACAATGTA+TGG | 0.487113 | 7.1:-16517472 | None:intergenic |
| ATGTTCTTATGCAACACAGC+CGG | 0.500939 | 7.1:+16517305 | MS.gene42572:CDS |
| TGGAATTGACAGGTTAAATC+CGG | 0.503112 | 7.1:-16517518 | None:intergenic |
| TCCAACTCCTCTGAATGCTC+GGG | 0.503319 | 7.1:-16518476 | None:intergenic |
| CTTTGATGTACAAGCGTGCT+AGG | 0.506024 | 7.1:-16517141 | None:intergenic |
| CTACAATTTCAAGATCTCAA+TGG | 0.512699 | 7.1:+16517363 | MS.gene42572:CDS |
| ATCCAGTACATTCTCAATGA+AGG | 0.513157 | 7.1:-16518730 | None:intergenic |
| GCCCGAGCATTCAGAGGAGT+TGG | 0.519376 | 7.1:+16518475 | MS.gene42572:CDS |
| CATCGTCAAACCGAAGTTGT+GGG | 0.523522 | 7.1:-16517219 | None:intergenic |
| GAGACTCTTACTTATACTGT+TGG | 0.541773 | 7.1:+16518611 | MS.gene42572:CDS |
| TGTTCTTATGCAACACAGCC+GGG | 0.544809 | 7.1:+16517306 | MS.gene42572:CDS |
| AGAAATCAGGTGGAATTGAC+AGG | 0.547647 | 7.1:-16517528 | None:intergenic |
| ACAAAATACTAGTCCTAACT+AGG | 0.547719 | 7.1:-16517165 | None:intergenic |
| TCGATCAATATGATCATGAC+TGG | 0.548556 | 7.1:-16517083 | None:intergenic |
| ATATTGAGCGTTTGAAGTAA+CGG | 0.549250 | 7.1:-16517634 | None:intergenic |
| TCACTATCTTAAGTCGATCA+AGG | 0.553035 | 7.1:-16518514 | None:intergenic |
| TTGTCTGTTACATTCGAGGC+CGG | 0.555309 | 7.1:+16517583 | MS.gene42572:CDS |
| GGACCTCCGTCCCACAACTT+CGG | 0.559824 | 7.1:+16517209 | MS.gene42572:CDS |
| TCATCGTCAAACCGAAGTTG+TGG | 0.564883 | 7.1:-16517220 | None:intergenic |
| ATGTCATCAATAACATTACA+TGG | 0.569407 | 7.1:-16517118 | None:intergenic |
| TCAATTCCACCTGATTTCTG+CGG | 0.580662 | 7.1:+16517532 | MS.gene42572:CDS |
| TCTTAAGTCGATCAAGGCAA+TGG | 0.584783 | 7.1:-16518508 | None:intergenic |
| CTCAATATTGATGATTGTTG+TGG | 0.587835 | 7.1:+16517649 | MS.gene42572:CDS |
| GCTGTGAATTTGAGTGAAAG+AGG | 0.588921 | 7.1:+16518281 | MS.gene42572:CDS |
| TTTGATCGAGATGTTTGTTG+TGG | 0.589034 | 7.1:-16517447 | None:intergenic |
| TATGACTCTGTCTAACAAGA+AGG | 0.594809 | 7.1:+16517060 | None:intergenic |
| AGCATGCAATGAGTCTGATA+CGG | 0.596787 | 7.1:+16518561 | MS.gene42572:CDS |
| TAGCCATAAGACTTTGAGAG+TGG | 0.604440 | 7.1:-16517698 | None:intergenic |
| GAGGAATGATATCTGCATAC+CGG | 0.607865 | 7.1:-16518208 | None:intergenic |
| TACGAGAATCTCGATGACCC+TGG | 0.632706 | 7.1:+16517256 | MS.gene42572:CDS |
| TTAAAGCCGCAGAAATCAGG+TGG | 0.633570 | 7.1:-16517538 | None:intergenic |
| AGTAAGAGTCTCTAACGATG+TGG | 0.634118 | 7.1:-16518600 | None:intergenic |
| CAATGGAATACATTGATACG+TGG | 0.634498 | 7.1:+16517380 | MS.gene42572:CDS |
| GCAAGCGCCCGAGCATTCAG+AGG | 0.637716 | 7.1:+16518469 | MS.gene42572:CDS |
| ACCTTAAGATCAACCAAGCG+AGG | 0.639054 | 7.1:-16518341 | None:intergenic |
| CTGTCAGCCTTACAATACCA+AGG | 0.642670 | 7.1:+16517840 | MS.gene42572:CDS |
| AGCCATAAGACTTTGAGAGT+GGG | 0.644910 | 7.1:-16517697 | None:intergenic |
| GTCAAACCGAAGTTGTGGGA+CGG | 0.658111 | 7.1:-16517215 | None:intergenic |
| GATGAAACTTATGCATCACC+CGG | 0.662686 | 7.1:-16517324 | None:intergenic |
| AATAAGACTCTCAAGTACAC+CGG | 0.679738 | 7.1:-16517602 | None:intergenic |
| AAACCGAAGTTGTGGGACGG+AGG | 0.683191 | 7.1:-16517212 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTATTATTTTTCAGTT+GGG | + | chr7.1:16517890-16517909 | MS.gene42572:intron | 10.0% |
| !!! | TATATTTATTATTTTTCAGT+TGG | + | chr7.1:16517889-16517908 | MS.gene42572:intron | 10.0% |
| !!! | TGATAATTTTAGAAGTTACA+GGG | - | chr7.1:16517282-16517301 | None:intergenic | 20.0% |
| !!! | TTCTAGTTGATATGTTATTT+TGG | + | chr7.1:16517918-16517937 | MS.gene42572:intron | 20.0% |
| ! | ATCTTGAAATTGTAGTCATA+AGG | - | chr7.1:16517358-16517377 | None:intergenic | 25.0% |
| ! | ATGTCATCAATAACATTACA+TGG | - | chr7.1:16517121-16517140 | None:intergenic | 25.0% |
| ! | GCATCAAATTCAAAATATTC+AGG | - | chr7.1:16517676-16517695 | None:intergenic | 25.0% |
| !! | CTAGTATTTTGTCAAAAAAG+TGG | + | chr7.1:16517176-16517195 | MS.gene42572:CDS | 25.0% |
| !!! | CAATGTTGAATTTTGATTGA+TGG | + | chr7.1:16518085-16518104 | MS.gene42572:intron | 25.0% |
| !!! | GTGATAATTTTAGAAGTTAC+AGG | - | chr7.1:16517283-16517302 | None:intergenic | 25.0% |
| ACAAAATACTAGTCCTAACT+AGG | - | chr7.1:16517168-16517187 | None:intergenic | 30.0% | |
| ACAACTTTCAATGTACATGT+TGG | - | chr7.1:16518117-16518136 | None:intergenic | 30.0% | |
| ATACATTGTCTTCTCTTGTA+AGG | + | chr7.1:16517474-16517493 | MS.gene42572:CDS | 30.0% | |
| ATATGCACAAACAACACTAT+GGG | - | chr7.1:16518158-16518177 | None:intergenic | 30.0% | |
| ATCCAATGACAGATACTTTA+TGG | - | chr7.1:16518261-16518280 | None:intergenic | 30.0% | |
| CTACAATTTCAAGATCTCAA+TGG | + | chr7.1:16517363-16517382 | MS.gene42572:CDS | 30.0% | |
| TAATACTCCTCGACAAAATA+GGG | - | chr7.1:16517408-16517427 | None:intergenic | 30.0% | |
| TTAATACTCCTCGACAAAAT+AGG | - | chr7.1:16517409-16517428 | None:intergenic | 30.0% | |
| TTACAAGAGAAGACAATGTA+TGG | - | chr7.1:16517475-16517494 | None:intergenic | 30.0% | |
| TTGACATTCTTTACATTTGC+CGG | + | chr7.1:16517499-16517518 | MS.gene42572:CDS | 30.0% | |
| TTTCAATGTACATGTTGGAT+TGG | - | chr7.1:16518112-16518131 | None:intergenic | 30.0% | |
| TTTCTAACCTTGGTATTGTA+AGG | - | chr7.1:16517850-16517869 | None:intergenic | 30.0% | |
| ! | ATATTGAGCGTTTGAAGTAA+CGG | - | chr7.1:16517637-16517656 | None:intergenic | 30.0% |
| ! | CTCAATATTGATGATTGTTG+TGG | + | chr7.1:16517649-16517668 | MS.gene42572:CDS | 30.0% |
| ! | GTATTTTGTCAAAAAAGTGG+AGG | + | chr7.1:16517179-16517198 | MS.gene42572:CDS | 30.0% |
| !!! | CTTACTTATACTGTTGGTTT+TGG | + | chr7.1:16518617-16518636 | MS.gene42572:CDS | 30.0% |
| !!! | GAAATCCTCTCAATGTTTTT+TGG | - | chr7.1:16517817-16517836 | None:intergenic | 30.0% |
| !!! | GTTTTGTTGTTTGTATGCAT+AGG | + | chr7.1:16518184-16518203 | MS.gene42572:intron | 30.0% |
| AATAAGACTCTCAAGTACAC+CGG | - | chr7.1:16517605-16517624 | None:intergenic | 35.0% | |
| CAATGGAATACATTGATACG+TGG | + | chr7.1:16517380-16517399 | MS.gene42572:CDS | 35.0% | |
| CTTATTGTCTGTTACATTCG+AGG | + | chr7.1:16517579-16517598 | MS.gene42572:CDS | 35.0% | |
| GAGACTCTTACTTATACTGT+TGG | + | chr7.1:16518611-16518630 | MS.gene42572:CDS | 35.0% | |
| GATATGCACAAACAACACTA+TGG | - | chr7.1:16518159-16518178 | None:intergenic | 35.0% | |
| GTTTGCCAAAAAACATTGAG+AGG | + | chr7.1:16517809-16517828 | MS.gene42572:CDS | 35.0% | |
| TCACTATCTTAAGTCGATCA+AGG | - | chr7.1:16518517-16518536 | None:intergenic | 35.0% | |
| TCGATCAATATGATCATGAC+TGG | - | chr7.1:16517086-16517105 | None:intergenic | 35.0% | |
| TGGAATTGACAGGTTAAATC+CGG | - | chr7.1:16517521-16517540 | None:intergenic | 35.0% | |
| TGTACATCAAAGACCTAGTT+AGG | + | chr7.1:16517152-16517171 | MS.gene42572:CDS | 35.0% | |
| ! | CAAAAAAGTGGAGGTATATG+TGG | + | chr7.1:16517188-16517207 | MS.gene42572:CDS | 35.0% |
| ! | CTCCATAAAGTATCTGTCAT+TGG | + | chr7.1:16518256-16518275 | MS.gene42572:CDS | 35.0% |
| ! | GCATTTCTGAAGAGTTGTTA+TGG | + | chr7.1:16518672-16518691 | MS.gene42572:CDS | 35.0% |
| ! | TTTGATCGAGATGTTTGTTG+TGG | - | chr7.1:16517450-16517469 | None:intergenic | 35.0% |
| !! | GTACTTGAGAGTCTTATTTC+TGG | + | chr7.1:16517607-16517626 | MS.gene42572:CDS | 35.0% |
| !!! | AATGAAGTTTTTAGCAGCGT+GGG | - | chr7.1:16518233-16518252 | None:intergenic | 35.0% |
| !!! | GTGATGTTGTTTTCTAACCT+TGG | - | chr7.1:16517860-16517879 | None:intergenic | 35.0% |
| AGAAATCAGGTGGAATTGAC+AGG | - | chr7.1:16517531-16517550 | None:intergenic | 40.0% | |
| AGCATGCAATGAGTCTGATA+CGG | + | chr7.1:16518561-16518580 | MS.gene42572:CDS | 40.0% | |
| AGTAAGAGTCTCTAACGATG+TGG | - | chr7.1:16518603-16518622 | None:intergenic | 40.0% | |
| ATCACCACTTCTATCTAGCT+TGG | + | chr7.1:16518000-16518019 | MS.gene42572:intron | 40.0% | |
| ATGTTCTTATGCAACACAGC+CGG | + | chr7.1:16517305-16517324 | MS.gene42572:CDS | 40.0% | |
| GAGGAATGATATCTGCATAC+CGG | - | chr7.1:16518211-16518230 | None:intergenic | 40.0% | |
| GATCTTAGACTCACAGTATC+TGG | + | chr7.1:16517775-16517794 | MS.gene42572:CDS | 40.0% | |
| GATGAAACTTATGCATCACC+CGG | - | chr7.1:16517327-16517346 | None:intergenic | 40.0% | |
| GCTGTGAATTTGAGTGAAAG+AGG | + | chr7.1:16518281-16518300 | MS.gene42572:CDS | 40.0% | |
| GTTGTTTGTATGCATAGGTC+CGG | + | chr7.1:16518189-16518208 | MS.gene42572:intron | 40.0% | |
| TCAATTCCACCTGATTTCTG+CGG | + | chr7.1:16517532-16517551 | MS.gene42572:CDS | 40.0% | |
| TCTTAAGTCGATCAAGGCAA+TGG | - | chr7.1:16518511-16518530 | None:intergenic | 40.0% | |
| ! | AGCCATAAGACTTTGAGAGT+GGG | - | chr7.1:16517700-16517719 | None:intergenic | 40.0% |
| ! | TAGCCATAAGACTTTGAGAG+TGG | - | chr7.1:16517701-16517720 | None:intergenic | 40.0% |
| !! | ATTTTAGAAGTTACAGGGCC+AGG | - | chr7.1:16517277-16517296 | None:intergenic | 40.0% |
| !! | TTTTAGAAGTTACAGGGCCA+GGG | - | chr7.1:16517276-16517295 | None:intergenic | 40.0% |
| !!! | CTTTTAAAGCCGCAGAAATC+AGG | - | chr7.1:16517544-16517563 | None:intergenic | 40.0% |
| !!! | GAATGAAGTTTTTAGCAGCG+TGG | - | chr7.1:16518234-16518253 | None:intergenic | 40.0% |
| ACCTTAAGATCAACCAAGCG+AGG | - | chr7.1:16518344-16518363 | None:intergenic | 45.0% | |
| CATCGTCAAACCGAAGTTGT+GGG | - | chr7.1:16517222-16517241 | None:intergenic | 45.0% | |
| CTCCCACTCTCAAAGTCTTA+TGG | + | chr7.1:16517695-16517714 | MS.gene42572:CDS | 45.0% | |
| CTGTCAGCCTTACAATACCA+AGG | + | chr7.1:16517840-16517859 | MS.gene42572:CDS | 45.0% | |
| CTTTGATGTACAAGCGTGCT+AGG | - | chr7.1:16517144-16517163 | None:intergenic | 45.0% | |
| GATGCCAAGCTAGATAGAAG+TGG | - | chr7.1:16518007-16518026 | None:intergenic | 45.0% | |
| TCATCGTCAAACCGAAGTTG+TGG | - | chr7.1:16517223-16517242 | None:intergenic | 45.0% | |
| TCCTCGCTTGGTTGATCTTA+AGG | + | chr7.1:16518340-16518359 | MS.gene42572:CDS | 45.0% | |
| TGTTCTTATGCAACACAGCC+GGG | + | chr7.1:16517306-16517325 | MS.gene42572:CDS | 45.0% | |
| TTAAAGCCGCAGAAATCAGG+TGG | - | chr7.1:16517541-16517560 | None:intergenic | 45.0% | |
| TTCCAACTCCTCTGAATGCT+CGG | - | chr7.1:16518480-16518499 | None:intergenic | 45.0% | |
| TTGTCTGTTACATTCGAGGC+CGG | + | chr7.1:16517583-16517602 | MS.gene42572:CDS | 45.0% | |
| ! | TTCTATCTAGCTTGGCATCG+TGG | + | chr7.1:16518008-16518027 | MS.gene42572:intron | 45.0% |
| !! | ACTTAAAAGTGCTCCTCGCT+TGG | + | chr7.1:16518328-16518347 | MS.gene42572:CDS | 45.0% |
| GTCAAACCGAAGTTGTGGGA+CGG | - | chr7.1:16517218-16517237 | None:intergenic | 50.0% | |
| TACGAGAATCTCGATGACCC+TGG | + | chr7.1:16517256-16517275 | MS.gene42572:CDS | 50.0% | |
| TCCAACTCCTCTGAATGCTC+GGG | - | chr7.1:16518479-16518498 | None:intergenic | 50.0% | |
| ! | CGTGGATCCCTATTTTGTCG+AGG | + | chr7.1:16517398-16517417 | MS.gene42572:CDS | 50.0% |
| !!! | GAAGTTTTTAGCAGCGTGGG+AGG | - | chr7.1:16518230-16518249 | None:intergenic | 50.0% |
| AAACCGAAGTTGTGGGACGG+AGG | - | chr7.1:16517215-16517234 | None:intergenic | 55.0% | |
| GCCCGAGCATTCAGAGGAGT+TGG | + | chr7.1:16518475-16518494 | MS.gene42572:CDS | 60.0% | |
| GGACCTCCGTCCCACAACTT+CGG | + | chr7.1:16517209-16517228 | MS.gene42572:CDS | 60.0% | |
| GCAAGCGCCCGAGCATTCAG+AGG | + | chr7.1:16518469-16518488 | MS.gene42572:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 16517061 | 16518739 | 16517061 | ID=MS.gene42572 |
| chr7.1 | mRNA | 16517061 | 16518739 | 16517061 | ID=MS.gene42572.t1;Parent=MS.gene42572 |
| chr7.1 | exon | 16517061 | 16517861 | 16517061 | ID=MS.gene42572.t1.exon1;Parent=MS.gene42572.t1 |
| chr7.1 | CDS | 16517061 | 16517861 | 16517061 | ID=cds.MS.gene42572.t1;Parent=MS.gene42572.t1 |
| chr7.1 | exon | 16518206 | 16518361 | 16518206 | ID=MS.gene42572.t1.exon2;Parent=MS.gene42572.t1 |
| chr7.1 | CDS | 16518206 | 16518361 | 16518206 | ID=cds.MS.gene42572.t1;Parent=MS.gene42572.t1 |
| chr7.1 | exon | 16518446 | 16518739 | 16518446 | ID=MS.gene42572.t1.exon3;Parent=MS.gene42572.t1 |
| chr7.1 | CDS | 16518446 | 16518739 | 16518446 | ID=cds.MS.gene42572.t1;Parent=MS.gene42572.t1 |
| Gene Sequence |
| Protein sequence |
