Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42679.t1 | XP_003603204.2 | 80.2 | 263 | 51 | 1 | 1 | 262 | 7 | 269 | 1.00E-96 | 363.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42679.t1 | G7J8E8 | 80.2 | 263 | 51 | 1 | 1 | 262 | 1 | 263 | 7.2e-97 | 363.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050584 | MS.gene42679 | 0.808147 | 3.57E-50 | -1.69E-46 |
| MS.gene05143 | MS.gene42679 | 0.816119 | 6.52E-52 | -1.69E-46 |
| MS.gene051789 | MS.gene42679 | 0.805468 | 1.32E-49 | -1.69E-46 |
| MS.gene052142 | MS.gene42679 | 0.822223 | 2.66E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene42679 | 0.831126 | 1.99E-55 | -1.69E-46 |
| MS.gene053074 | MS.gene42679 | 0.866678 | 2.39E-65 | -1.69E-46 |
| MS.gene054596 | MS.gene42679 | 0.830093 | 3.57E-55 | -1.69E-46 |
| MS.gene055359 | MS.gene42679 | 0.869655 | 2.63E-66 | -1.69E-46 |
| MS.gene058168 | MS.gene42679 | 0.837094 | 6.37E-57 | -1.69E-46 |
| MS.gene059006 | MS.gene42679 | 0.868575 | 5.90E-66 | -1.69E-46 |
| MS.gene059124 | MS.gene42679 | 0.811915 | 5.51E-51 | -1.69E-46 |
| MS.gene059632 | MS.gene42679 | 0.817445 | 3.29E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42679.t1 | MTR_3g105010 | 93.536 | 263 | 16 | 1 | 1 | 262 | 1 | 263 | 3.31e-176 | 486 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42679.t1 | AT1G76880 | 39.474 | 76 | 46 | 0 | 181 | 256 | 256 | 331 | 7.93e-11 | 62.4 |
Find 41 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTTTGGGGTTAAGATATT+TGG | 0.163691 | 3.3:+86391667 | None:intergenic |
| ACAACAACAAACACATTCTT+TGG | 0.164595 | 3.3:-86391503 | MS.gene42679:CDS |
| GATGCGAAAGAAGAGATTTA+AGG | 0.234830 | 3.3:-86390796 | MS.gene42679:CDS |
| GGATACAGAGGGAAGAAAAT+TGG | 0.293718 | 3.3:-86390659 | MS.gene42679:CDS |
| TTCTTGATAAAGGATGTTAT+TGG | 0.304540 | 3.3:+86391732 | None:intergenic |
| TGAGTTTCTGTTCTTGATAA+AGG | 0.309215 | 3.3:+86391722 | None:intergenic |
| TAGGGTGATGAATGTGGTTT+GGG | 0.329828 | 3.3:+86391653 | None:intergenic |
| TCCAGCATTGAGAAAATAAC+TGG | 0.347658 | 3.3:-86390546 | MS.gene42679:CDS |
| AATGATAGTGTTGGAGAATA+GGG | 0.371956 | 3.3:+86391635 | None:intergenic |
| GGAAAACAACGAGAGATGTT+TGG | 0.402412 | 3.3:-86390919 | MS.gene42679:CDS |
| ATTTGGAGGGTTGTTGAGAA+TGG | 0.405149 | 3.3:+86391684 | None:intergenic |
| TTGGTGATTAACCACAGTTT+TGG | 0.419669 | 3.3:+86390734 | None:intergenic |
| TAATGATAGTGTTGGAGAAT+AGG | 0.423149 | 3.3:+86391634 | None:intergenic |
| CAACAACAAACACATTCTTT+GGG | 0.431558 | 3.3:-86391502 | MS.gene42679:CDS |
| GGTTCTTCAGAAGAAATTGT+TGG | 0.438181 | 3.3:-86390709 | MS.gene42679:CDS |
| TGAGAATGGAACCATCATCT+AGG | 0.456014 | 3.3:+86391698 | None:intergenic |
| TTGGTTTCCGACAACAACTT+TGG | 0.462012 | 3.3:-86390900 | MS.gene42679:CDS |
| ATAGGGTGATGAATGTGGTT+TGG | 0.467705 | 3.3:+86391652 | None:intergenic |
| GTTGGAGGTGATTGATAGAA+TGG | 0.468582 | 3.3:-86390691 | MS.gene42679:CDS |
| TTGATGGTTGAAATTTATGT+TGG | 0.470837 | 3.3:+86391527 | None:intergenic |
| ATTTCTTCTGAAGAACCTCT+TGG | 0.473081 | 3.3:+86390715 | None:intergenic |
| AAGCTCACCAAAGTTGTTGT+CGG | 0.473825 | 3.3:+86390893 | None:intergenic |
| CTTGAAGCTGTGTACAAACT+TGG | 0.499197 | 3.3:-86390873 | MS.gene42679:CDS |
| TTTGGGGTTAAGATATTTGG+AGG | 0.499198 | 3.3:+86391670 | None:intergenic |
| AGGAACATGATTCGAAGAGA+AGG | 0.540619 | 3.3:-86390830 | MS.gene42679:CDS |
| CAGAAACTCACCCTAGATGA+TGG | 0.541007 | 3.3:-86391709 | MS.gene42679:CDS |
| ATGATTCGAAGAGAAGGGCT+AGG | 0.550539 | 3.3:-86390824 | MS.gene42679:CDS |
| TCTTCAGAAGAAATTGTTGG+AGG | 0.567079 | 3.3:-86390706 | MS.gene42679:CDS |
| GAGAATGGAACCATCATCTA+GGG | 0.574060 | 3.3:+86391699 | None:intergenic |
| GGAACATGATTCGAAGAGAA+GGG | 0.586343 | 3.3:-86390829 | MS.gene42679:CDS |
| GGAGAATAGGGTGATGAATG+TGG | 0.595952 | 3.3:+86391647 | None:intergenic |
| TTGGGGTTAAGATATTTGGA+GGG | 0.596919 | 3.3:+86391671 | None:intergenic |
| TAAAAGATTGACCAAAACTG+TGG | 0.602456 | 3.3:-86390745 | MS.gene42679:CDS |
| TACAGAGGGAAGAAAATTGG+AGG | 0.605765 | 3.3:-86390656 | MS.gene42679:CDS |
| CAGGAAAGCATTCAACAACA+CGG | 0.615004 | 3.3:-86390979 | MS.gene42679:CDS |
| AATCGTTACATCGAATGCGA+AGG | 0.628296 | 3.3:-86391466 | MS.gene42679:CDS |
| ACACACAACAACAGAAAGAA+CGG | 0.629520 | 3.3:-86391594 | MS.gene42679:CDS |
| ATTGTGAGTAATGATAGTGT+TGG | 0.653355 | 3.3:+86391626 | None:intergenic |
| AACTGTGGTTAATCACCAAG+AGG | 0.665729 | 3.3:-86390730 | MS.gene42679:CDS |
| AGGGTGATGAATGTGGTTTG+GGG | 0.692532 | 3.3:+86391654 | None:intergenic |
| TCAGAATCAAGCGTTCACTG+AGG | 0.786263 | 3.3:-86390850 | MS.gene42679:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTCATTAAAAATATTTATCA+AGG | + | chr3.3:86391178-86391197 | None:intergenic | 15.0% |
| !! | AATATTTATCAAGGATTGAT+AGG | + | chr3.3:86391169-86391188 | None:intergenic | 20.0% |
| !!! | ACATTTTTATCAATCATATG+AGG | - | chr3.3:86390709-86390728 | MS.gene42679:CDS | 20.0% |
| ! | ACAAACATACATAATGTGAT+AGG | + | chr3.3:86391253-86391272 | None:intergenic | 25.0% |
| ! | AGAAAAACAACAACATGAAA+AGG | - | chr3.3:86391323-86391342 | MS.gene42679:intron | 25.0% |
| ! | CAGAACATGTTATATAATGT+GGG | + | chr3.3:86390979-86390998 | None:intergenic | 25.0% |
| ! | GAATTACCAAAAAGATACTT+TGG | + | chr3.3:86390831-86390850 | None:intergenic | 25.0% |
| ! | TCAGAACATGTTATATAATG+TGG | + | chr3.3:86390980-86390999 | None:intergenic | 25.0% |
| ! | TTCTTGATAAAGGATGTTAT+TGG | + | chr3.3:86390534-86390553 | None:intergenic | 25.0% |
| ! | TTGATAGAATGGAAAAAGAA+AGG | - | chr3.3:86391583-86391602 | MS.gene42679:CDS | 25.0% |
| !! | TTGATGGTTGAAATTTATGT+TGG | + | chr3.3:86390739-86390758 | None:intergenic | 25.0% |
| !!! | GGTGTTAGTTTTATAAAGAA+AGG | + | chr3.3:86390946-86390965 | None:intergenic | 25.0% |
| ACAACAACAAACACATTCTT+TGG | - | chr3.3:86390760-86390779 | MS.gene42679:CDS | 30.0% | |
| CAACAACAAACACATTCTTT+GGG | - | chr3.3:86390761-86390780 | MS.gene42679:CDS | 30.0% | |
| CAAGACCAATACATCAATAT+GGG | + | chr3.3:86391079-86391098 | None:intergenic | 30.0% | |
| CACATTATGTATGTTTGTGT+TGG | - | chr3.3:86391255-86391274 | MS.gene42679:intron | 30.0% | |
| CAGATTTGTACATTTATTCC+GGG | + | chr3.3:86391018-86391037 | None:intergenic | 30.0% | |
| CCAGATTTGTACATTTATTC+CGG | + | chr3.3:86391019-86391038 | None:intergenic | 30.0% | |
| TAAAAGATTGACCAAAACTG+TGG | - | chr3.3:86391518-86391537 | MS.gene42679:CDS | 30.0% | |
| TACTACCCATATTGATGTAT+TGG | - | chr3.3:86391071-86391090 | MS.gene42679:intron | 30.0% | |
| TGAGTTTCTGTTCTTGATAA+AGG | + | chr3.3:86390544-86390563 | None:intergenic | 30.0% | |
| TTGATAGGCAAATAGATCAT+TGG | + | chr3.3:86391154-86391173 | None:intergenic | 30.0% | |
| ! | AATGATAGTGTTGGAGAATA+GGG | + | chr3.3:86390631-86390650 | None:intergenic | 30.0% |
| ! | ATTGTGAGTAATGATAGTGT+TGG | + | chr3.3:86390640-86390659 | None:intergenic | 30.0% |
| ! | TAATGATAGTGTTGGAGAAT+AGG | + | chr3.3:86390632-86390651 | None:intergenic | 30.0% |
| !!! | TGTGTTTGTTGTTGTTTTGA+TGG | + | chr3.3:86390755-86390774 | None:intergenic | 30.0% |
| ACACACAACAACAGAAAGAA+CGG | - | chr3.3:86390669-86390688 | MS.gene42679:CDS | 35.0% | |
| CCAAAAAGATACTTTGGCAT+TGG | + | chr3.3:86390825-86390844 | None:intergenic | 35.0% | |
| CCGGAATAAATGTACAAATC+TGG | - | chr3.3:86391016-86391035 | MS.gene42679:intron | 35.0% | |
| GATGCGAAAGAAGAGATTTA+AGG | - | chr3.3:86391467-86391486 | MS.gene42679:CDS | 35.0% | |
| GCAAGACCAATACATCAATA+TGG | + | chr3.3:86391080-86391099 | None:intergenic | 35.0% | |
| GCAATTAGTACAGGTGATAT+AGG | - | chr3.3:86391128-86391147 | MS.gene42679:intron | 35.0% | |
| GGAAAAAGAAAGGATACAGA+GGG | - | chr3.3:86391593-86391612 | MS.gene42679:CDS | 35.0% | |
| GGTTCTTCAGAAGAAATTGT+TGG | - | chr3.3:86391554-86391573 | MS.gene42679:CDS | 35.0% | |
| TATAATGTGGGTGTATATGC+TGG | + | chr3.3:86390967-86390986 | None:intergenic | 35.0% | |
| TCTTCAGAAGAAATTGTTGG+AGG | - | chr3.3:86391557-86391576 | MS.gene42679:CDS | 35.0% | |
| TGGAAAAAGAAAGGATACAG+AGG | - | chr3.3:86391592-86391611 | MS.gene42679:CDS | 35.0% | |
| TTGGGGTTAAGATATTTGGA+GGG | + | chr3.3:86390595-86390614 | None:intergenic | 35.0% | |
| ! | ATTTCTTCTGAAGAACCTCT+TGG | + | chr3.3:86391551-86391570 | None:intergenic | 35.0% |
| ! | TCCAGCATTGAGAAAATAAC+TGG | - | chr3.3:86391717-86391736 | MS.gene42679:CDS | 35.0% |
| ! | TGGTTTGGGGTTAAGATATT+TGG | + | chr3.3:86390599-86390618 | None:intergenic | 35.0% |
| ! | TTTGGGGTTAAGATATTTGG+AGG | + | chr3.3:86390596-86390615 | None:intergenic | 35.0% |
| !! | ACCAGTTATTTTCTCAATGC+TGG | + | chr3.3:86391721-86391740 | None:intergenic | 35.0% |
| !! | ATGTTTGTGTTGGCATTTTC+AGG | - | chr3.3:86391265-86391284 | MS.gene42679:intron | 35.0% |
| !! | CCAATGCCAAAGTATCTTTT+TGG | - | chr3.3:86390822-86390841 | MS.gene42679:CDS | 35.0% |
| !! | TTGGTGATTAACCACAGTTT+TGG | + | chr3.3:86391532-86391551 | None:intergenic | 35.0% |
| AACTGTGGTTAATCACCAAG+AGG | - | chr3.3:86391533-86391552 | MS.gene42679:CDS | 40.0% | |
| AAGCTCACCAAAGTTGTTGT+CGG | + | chr3.3:86391373-86391392 | None:intergenic | 40.0% | |
| AATCGTTACATCGAATGCGA+AGG | - | chr3.3:86390797-86390816 | MS.gene42679:CDS | 40.0% | |
| AGGAACATGATTCGAAGAGA+AGG | - | chr3.3:86391433-86391452 | MS.gene42679:intron | 40.0% | |
| ATAGGGTGATGAATGTGGTT+TGG | + | chr3.3:86390614-86390633 | None:intergenic | 40.0% | |
| ATTTGGAGGGTTGTTGAGAA+TGG | + | chr3.3:86390582-86390601 | None:intergenic | 40.0% | |
| CTTGAAGCTGTGTACAAACT+TGG | - | chr3.3:86391390-86391409 | MS.gene42679:intron | 40.0% | |
| GAGAATGGAACCATCATCTA+GGG | + | chr3.3:86390567-86390586 | None:intergenic | 40.0% | |
| GGAAAACAACGAGAGATGTT+TGG | - | chr3.3:86391344-86391363 | MS.gene42679:intron | 40.0% | |
| GGAACATGATTCGAAGAGAA+GGG | - | chr3.3:86391434-86391453 | MS.gene42679:intron | 40.0% | |
| GGATACAGAGGGAAGAAAAT+TGG | - | chr3.3:86391604-86391623 | MS.gene42679:CDS | 40.0% | |
| GTTGGAGGTGATTGATAGAA+TGG | - | chr3.3:86391572-86391591 | MS.gene42679:CDS | 40.0% | |
| TACAGAGGGAAGAAAATTGG+AGG | - | chr3.3:86391607-86391626 | MS.gene42679:CDS | 40.0% | |
| TAGGGTGATGAATGTGGTTT+GGG | + | chr3.3:86390613-86390632 | None:intergenic | 40.0% | |
| TGAGAATGGAACCATCATCT+AGG | + | chr3.3:86390568-86390587 | None:intergenic | 40.0% | |
| TTCGCTTTCGCAATTAGTAC+AGG | - | chr3.3:86391119-86391138 | MS.gene42679:intron | 40.0% | |
| TTGGTTTCCGACAACAACTT+TGG | - | chr3.3:86391363-86391382 | MS.gene42679:intron | 40.0% | |
| TTTATTCCGGGCTCATTGTA+CGG | + | chr3.3:86391006-86391025 | None:intergenic | 40.0% | |
| ! | GTACAAATCTGGTTAGCTCA+TGG | - | chr3.3:86391027-86391046 | MS.gene42679:intron | 40.0% |
| ! | GTGAGCGTGATTTTGCTAAA+CGG | - | chr3.3:86391676-86391695 | MS.gene42679:CDS | 40.0% |
| !! | CAGGAAAGCATTCAACAACA+CGG | - | chr3.3:86391284-86391303 | MS.gene42679:intron | 40.0% |
| AGGGTGATGAATGTGGTTTG+GGG | + | chr3.3:86390612-86390631 | None:intergenic | 45.0% | |
| ATGATTCGAAGAGAAGGGCT+AGG | - | chr3.3:86391439-86391458 | MS.gene42679:intron | 45.0% | |
| CAGAAACTCACCCTAGATGA+TGG | - | chr3.3:86390554-86390573 | MS.gene42679:CDS | 45.0% | |
| GGAGAATAGGGTGATGAATG+TGG | + | chr3.3:86390619-86390638 | None:intergenic | 45.0% | |
| TCAGAATCAAGCGTTCACTG+AGG | - | chr3.3:86391413-86391432 | MS.gene42679:intron | 45.0% | |
| TGATAACCGTACAATGAGCC+CGG | - | chr3.3:86390997-86391016 | MS.gene42679:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 86390527 | 86391758 | 86390527 | ID=MS.gene42679 |
| chr3.3 | mRNA | 86390527 | 86391758 | 86390527 | ID=MS.gene42679.t1;Parent=MS.gene42679 |
| chr3.3 | exon | 86391442 | 86391758 | 86391442 | ID=MS.gene42679.t1.exon1;Parent=MS.gene42679.t1 |
| chr3.3 | CDS | 86391442 | 86391758 | 86391442 | ID=cds.MS.gene42679.t1;Parent=MS.gene42679.t1 |
| chr3.3 | exon | 86390527 | 86390998 | 86390527 | ID=MS.gene42679.t1.exon2;Parent=MS.gene42679.t1 |
| chr3.3 | CDS | 86390527 | 86390998 | 86390527 | ID=cds.MS.gene42679.t1;Parent=MS.gene42679.t1 |
| Gene Sequence |
| Protein sequence |
