Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42983.t1 | RHN40757.1 | 95.7 | 92 | 3 | 1 | 45 | 135 | 22 | 113 | 4.40E-40 | 174.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42983.t1 | A0A396GPW6 | 95.7 | 92 | 3 | 1 | 45 | 135 | 22 | 113 | 3.2e-40 | 174.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene42983 | 0.802619 | 5.15E-49 | -1.69E-46 |
| MS.gene050224 | MS.gene42983 | 0.800016 | 1.76E-48 | -1.69E-46 |
| MS.gene050414 | MS.gene42983 | 0.800191 | 1.62E-48 | -1.69E-46 |
| MS.gene052016 | MS.gene42983 | 0.801044 | 1.08E-48 | -1.69E-46 |
| MS.gene052307 | MS.gene42983 | 0.802052 | 6.74E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene42983 | 0.804764 | 1.85E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene42983 | 0.805607 | 1.23E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene42983 | 0.812131 | 4.95E-51 | -1.69E-46 |
| MS.gene058159 | MS.gene42983 | 0.809378 | 1.95E-50 | -1.69E-46 |
| MS.gene058330 | MS.gene42983 | 0.800099 | 1.69E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene42983 | 0.807958 | 3.92E-50 | -1.69E-46 |
| MS.gene059634 | MS.gene42983 | 0.814506 | 1.49E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene42983 | 0.826529 | 2.58E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42983.t1 | MTR_2g069350 | 36.145 | 166 | 62 | 7 | 4 | 135 | 1 | 156 | 6.37e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCACTGTTACCACTTTC+AGG | 0.214320 | 8.1:+54774638 | MS.gene42983:CDS |
| GTTGAAGTTGAGAAGGAAAA+AGG | 0.285209 | 8.1:-54774436 | None:intergenic |
| AGGGTAGTTATTGTTATTGA+TGG | 0.337872 | 8.1:-54774819 | None:intergenic |
| CTTCTCAACTTCAACCCAAT+AGG | 0.388096 | 8.1:+54774444 | MS.gene42983:CDS |
| AAGATTGTACTATACCTATT+GGG | 0.415314 | 8.1:-54774458 | None:intergenic |
| GAAGATGGTGTCTGGTTTCA+CGG | 0.465973 | 8.1:-54774750 | None:intergenic |
| ACGGAGGTAATCGGTTATGA+AGG | 0.473012 | 8.1:-54774714 | None:intergenic |
| TTGTAAGCGAAGATGGTGTC+TGG | 0.480918 | 8.1:-54774758 | None:intergenic |
| AACGTTGTTGTAAGCGAAGA+TGG | 0.493808 | 8.1:-54774765 | None:intergenic |
| CGGAGGTAATCGGTTATGAA+GGG | 0.544643 | 8.1:-54774713 | None:intergenic |
| GTTATGAAGGGGTTGTTCGA+CGG | 0.547493 | 8.1:-54774701 | None:intergenic |
| GTTGAGATGGTGAGCATCGA+CGG | 0.556642 | 8.1:-54774414 | None:intergenic |
| CATGTTTGTATAATTTGTGT+CGG | 0.569763 | 8.1:-54774603 | None:intergenic |
| CTATTGGGTTGAAGTTGAGA+AGG | 0.576004 | 8.1:-54774443 | None:intergenic |
| ACGGTGGTTGTGCCTGAAAG+TGG | 0.615359 | 8.1:-54774650 | None:intergenic |
| TTGTTCGACGGAGATTGCGA+CGG | 0.617379 | 8.1:-54774689 | None:intergenic |
| TTCAGGCACAACCACCGTGT+CGG | 0.634510 | 8.1:+54774655 | MS.gene42983:CDS |
| AGAAATGAAGTTGTCGATGT+CGG | 0.644465 | 8.1:-54774885 | None:intergenic |
| GGAGGTAATCGGTTATGAAG+GGG | 0.660773 | 8.1:-54774712 | None:intergenic |
| GATGGTGTCTGGTTTCACGG+TGG | 0.760177 | 8.1:-54774747 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAACAAAACAAAAAACA+TGG | - | chr8.1:54774520-54774539 | None:intergenic | 20.0% |
| ! | AAAAATCTTCACTGATGAAA+GGG | - | chr8.1:54774841-54774860 | None:intergenic | 25.0% |
| ! | AAGATTGTACTATACCTATT+GGG | - | chr8.1:54774461-54774480 | None:intergenic | 25.0% |
| ! | CAAAACAAAAAACATGGTTT+AGG | - | chr8.1:54774514-54774533 | None:intergenic | 25.0% |
| !! | CATGTTTGTATAATTTGTGT+CGG | - | chr8.1:54774606-54774625 | None:intergenic | 25.0% |
| GAAAAATCTTCACTGATGAA+AGG | - | chr8.1:54774842-54774861 | None:intergenic | 30.0% | |
| GAAGATTGTACTATACCTAT+TGG | - | chr8.1:54774462-54774481 | None:intergenic | 30.0% | |
| ! | AGGGTAGTTATTGTTATTGA+TGG | - | chr8.1:54774822-54774841 | None:intergenic | 30.0% |
| !!! | AATCTCTAGAACGTTTTTGT+GGG | - | chr8.1:54774799-54774818 | None:intergenic | 30.0% |
| !!! | TATTTTTTGACGGAGGTAAT+CGG | - | chr8.1:54774726-54774745 | None:intergenic | 30.0% |
| AGAAATGAAGTTGTCGATGT+CGG | - | chr8.1:54774888-54774907 | None:intergenic | 35.0% | |
| GTTGAAGTTGAGAAGGAAAA+AGG | - | chr8.1:54774439-54774458 | None:intergenic | 35.0% | |
| !!! | GAATCTCTAGAACGTTTTTG+TGG | - | chr8.1:54774800-54774819 | None:intergenic | 35.0% |
| !!! | TCACGGTGGATATTTTTTGA+CGG | - | chr8.1:54774736-54774755 | None:intergenic | 35.0% |
| AACGTTGTTGTAAGCGAAGA+TGG | - | chr8.1:54774768-54774787 | None:intergenic | 40.0% | |
| CTTCTCAACTTCAACCCAAT+AGG | + | chr8.1:54774444-54774463 | MS.gene42983:CDS | 40.0% | |
| GAGAAGGAAAAAGGTTGAGA+TGG | - | chr8.1:54774430-54774449 | None:intergenic | 40.0% | |
| TCATCACTGTTACCACTTTC+AGG | + | chr8.1:54774638-54774657 | MS.gene42983:CDS | 40.0% | |
| !! | CTATTGGGTTGAAGTTGAGA+AGG | - | chr8.1:54774446-54774465 | None:intergenic | 40.0% |
| ACGGAGGTAATCGGTTATGA+AGG | - | chr8.1:54774717-54774736 | None:intergenic | 45.0% | |
| CGGAGGTAATCGGTTATGAA+GGG | - | chr8.1:54774716-54774735 | None:intergenic | 45.0% | |
| GGAGGTAATCGGTTATGAAG+GGG | - | chr8.1:54774715-54774734 | None:intergenic | 45.0% | |
| GTTATGAAGGGGTTGTTCGA+CGG | - | chr8.1:54774704-54774723 | None:intergenic | 45.0% | |
| TTGTAAGCGAAGATGGTGTC+TGG | - | chr8.1:54774761-54774780 | None:intergenic | 45.0% | |
| ! | AATGAGATTTTCCGACACGG+TGG | - | chr8.1:54774669-54774688 | None:intergenic | 45.0% |
| ! | CGGAATGAGATTTTCCGACA+CGG | - | chr8.1:54774672-54774691 | None:intergenic | 45.0% |
| ! | GAAGATGGTGTCTGGTTTCA+CGG | - | chr8.1:54774753-54774772 | None:intergenic | 45.0% |
| !! | TGTCGATGTCGGTGTTTTGA+TGG | - | chr8.1:54774877-54774896 | None:intergenic | 45.0% |
| !!! | CGGTGGATATTTTTTGACGG+AGG | - | chr8.1:54774733-54774752 | None:intergenic | 45.0% |
| TTGTTCGACGGAGATTGCGA+CGG | - | chr8.1:54774692-54774711 | None:intergenic | 50.0% | |
| !! | GTTGAGATGGTGAGCATCGA+CGG | - | chr8.1:54774417-54774436 | None:intergenic | 50.0% |
| !!! | CGATGTCGGTGTTTTGATGG+TGG | - | chr8.1:54774874-54774893 | None:intergenic | 50.0% |
| ACGGTGGTTGTGCCTGAAAG+TGG | - | chr8.1:54774653-54774672 | None:intergenic | 55.0% | |
| TTCAGGCACAACCACCGTGT+CGG | + | chr8.1:54774655-54774674 | MS.gene42983:CDS | 55.0% | |
| ! | GATGGTGTCTGGTTTCACGG+TGG | - | chr8.1:54774750-54774769 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 54774401 | 54774913 | 54774401 | ID=MS.gene42983 |
| chr8.1 | mRNA | 54774401 | 54774913 | 54774401 | ID=MS.gene42983.t1;Parent=MS.gene42983 |
| chr8.1 | exon | 54774401 | 54774465 | 54774401 | ID=MS.gene42983.t1.exon1;Parent=MS.gene42983.t1 |
| chr8.1 | CDS | 54774401 | 54774465 | 54774401 | ID=cds.MS.gene42983.t1;Parent=MS.gene42983.t1 |
| chr8.1 | exon | 54774574 | 54774913 | 54774574 | ID=MS.gene42983.t1.exon2;Parent=MS.gene42983.t1 |
| chr8.1 | CDS | 54774574 | 54774913 | 54774574 | ID=cds.MS.gene42983.t1;Parent=MS.gene42983.t1 |
| Gene Sequence |
| Protein sequence |
