AlfalfaGEDB | MS.gene050224

M. sativa cultivar XinJiangDaYe / MS.gene050224

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050224.t1	KEH43388.1	92.2	153	12	0	1	153	261	413	3.30E-60	241.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050224.t1	Q0WQF7	56.9	153	66	0	1	153	265	417	3.0e-25	116.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050224.t1	A0A072VN38	92.2	153	12	0	1	153	253	405	2.4e-60	241.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene050224	0.804706	1.90E-49	-1.69E-46
MS.gene049807	MS.gene050224	0.808858	2.52E-50	-1.69E-46
MS.gene050224	MS.gene050359	0.820552	6.46E-53	-1.69E-46
MS.gene050224	MS.gene051102	0.800605	1.33E-48	-1.69E-46
MS.gene050224	MS.gene051381	0.80081	1.21E-48	-1.69E-46
MS.gene050224	MS.gene051724	0.801988	6.94E-49	-1.69E-46
MS.gene050224	MS.gene052307	0.84338	1.45E-58	-1.69E-46
MS.gene050224	MS.gene052609	0.874115	8.69E-68	-1.69E-46
MS.gene050224	MS.gene053143	0.831578	1.54E-55	-1.69E-46
MS.gene050224	MS.gene054585	0.81013	1.34E-50	-1.69E-46
MS.gene050224	MS.gene056507	0.806111	9.64E-50	-1.69E-46
MS.gene050224	MS.gene058649	0.810653	1.04E-50	-1.69E-46
MS.gene050224	MS.gene061100	0.800893	1.16E-48	-1.69E-46
MS.gene050224	MS.gene06133	0.801631	8.22E-49	-1.69E-46
MS.gene050224	MS.gene061707	0.802303	5.98E-49	-1.69E-46
MS.gene050224	MS.gene063149	0.809275	2.05E-50	-1.69E-46
MS.gene050224	MS.gene064243	0.83585	1.32E-56	-1.69E-46
MS.gene050224	MS.gene065593	0.838615	2.59E-57	-1.69E-46
MS.gene050224	MS.gene066196	0.823878	1.09E-53	-1.69E-46
MS.gene050224	MS.gene066897	0.839101	1.94E-57	-1.69E-46
MS.gene050224	MS.gene067773	0.800129	1.67E-48	-1.69E-46
MS.gene050224	MS.gene067964	0.824353	8.45E-54	-1.69E-46
MS.gene050224	MS.gene067966	0.828289	9.77E-55	-1.69E-46
MS.gene050224	MS.gene067967	0.834828	2.39E-56	-1.69E-46
MS.gene050224	MS.gene072116	0.80819	3.50E-50	-1.69E-46
MS.gene050224	MS.gene07382	0.832853	7.46E-56	-1.69E-46
MS.gene050224	MS.gene08325	0.821801	3.33E-53	-1.69E-46
MS.gene050224	MS.gene08887	0.830111	3.53E-55	-1.69E-46
MS.gene050224	MS.gene09151	0.805621	1.22E-49	-1.69E-46
MS.gene050224	MS.gene21826	0.825427	4.72E-54	-1.69E-46
MS.gene050224	MS.gene23078	0.802475	5.51E-49	-1.69E-46
MS.gene050224	MS.gene24827	0.811575	6.54E-51	-1.69E-46
MS.gene050224	MS.gene24903	0.812461	4.19E-51	-1.69E-46
MS.gene050224	MS.gene25215	0.802713	4.92E-49	-1.69E-46
MS.gene050224	MS.gene25217	0.805784	1.13E-49	-1.69E-46
MS.gene050224	MS.gene26396	0.813382	2.63E-51	-1.69E-46
MS.gene050224	MS.gene27119	0.823035	1.72E-53	-1.69E-46
MS.gene050224	MS.gene27700	0.808223	3.44E-50	-1.69E-46
MS.gene050224	MS.gene28924	0.802158	6.41E-49	-1.69E-46
MS.gene050224	MS.gene28979	0.822832	1.92E-53	-1.69E-46
MS.gene050224	MS.gene30419	0.805206	1.49E-49	-1.69E-46
MS.gene050224	MS.gene32685	0.804617	1.98E-49	-1.69E-46
MS.gene050224	MS.gene34057	0.809818	1.57E-50	-1.69E-46
MS.gene050224	MS.gene34286	0.809228	2.10E-50	-1.69E-46
MS.gene050224	MS.gene34453	0.813975	1.95E-51	-1.69E-46
MS.gene050224	MS.gene34485	0.813012	3.17E-51	-1.69E-46
MS.gene050224	MS.gene34937	0.832081	1.16E-55	-1.69E-46
MS.gene050224	MS.gene35800	0.800354	1.50E-48	-1.69E-46
MS.gene050224	MS.gene35934	0.811105	8.27E-51	-1.69E-46
MS.gene050224	MS.gene36285	0.83447	2.94E-56	-1.69E-46
MS.gene050224	MS.gene36291	0.810968	8.85E-51	-1.69E-46
MS.gene050224	MS.gene36992	0.801623	8.25E-49	-1.69E-46
MS.gene050224	MS.gene37007	0.818689	1.72E-52	-1.69E-46
MS.gene050224	MS.gene37011	0.808743	2.67E-50	-1.69E-46
MS.gene050224	MS.gene37106	0.815763	7.82E-52	-1.69E-46
MS.gene050224	MS.gene38302	0.822015	2.97E-53	-1.69E-46
MS.gene050224	MS.gene38303	0.813943	1.98E-51	-1.69E-46
MS.gene050224	MS.gene39046	0.861656	8.80E-64	-1.69E-46
MS.gene050224	MS.gene39124	0.815344	9.70E-52	-1.69E-46
MS.gene050224	MS.gene40093	0.802378	5.77E-49	-1.69E-46
MS.gene050224	MS.gene41427	0.845897	3.05E-59	-1.69E-46
MS.gene050224	MS.gene41692	0.81467	1.37E-51	-1.69E-46
MS.gene050224	MS.gene42528	0.853823	1.85E-61	-1.69E-46
MS.gene050224	MS.gene42783	0.832045	1.18E-55	-1.69E-46
MS.gene050224	MS.gene42983	0.800016	1.76E-48	-1.69E-46
MS.gene050224	MS.gene44326	0.819575	1.08E-52	-1.69E-46
MS.gene050224	MS.gene45281	0.823998	1.02E-53	-1.69E-46
MS.gene050224	MS.gene45624	0.80796	3.92E-50	-1.69E-46
MS.gene050224	MS.gene47554	0.812592	3.92E-51	-1.69E-46
MS.gene050224	MS.gene47581	0.819741	9.90E-53	-1.69E-46
MS.gene050224	MS.gene47601	0.837119	6.27E-57	-1.69E-46
MS.gene050224	MS.gene47806	0.808987	2.36E-50	-1.69E-46
MS.gene050224	MS.gene48283	0.810661	1.03E-50	-1.69E-46
MS.gene050224	MS.gene49383	0.811111	8.24E-51	-1.69E-46
MS.gene050224	MS.gene49467	0.811761	5.96E-51	-1.69E-46
MS.gene050224	MS.gene49818	0.806724	7.16E-50	-1.69E-46
MS.gene050224	MS.gene51029	0.801949	7.07E-49	-1.69E-46
MS.gene050224	MS.gene51384	0.834629	2.68E-56	-1.69E-46
MS.gene050224	MS.gene51929	0.823212	1.56E-53	-1.69E-46
MS.gene050224	MS.gene54158	0.821011	5.06E-53	-1.69E-46
MS.gene050224	MS.gene54548	0.806494	8.00E-50	-1.69E-46
MS.gene050224	MS.gene56476	0.817643	2.97E-52	-1.69E-46
MS.gene050224	MS.gene59241	0.801655	8.13E-49	-1.69E-46
MS.gene050224	MS.gene59242	0.804732	1.88E-49	-1.69E-46
MS.gene050224	MS.gene59366	0.827656	1.39E-54	-1.69E-46
MS.gene050224	MS.gene59499	0.81605	6.75E-52	-1.69E-46
MS.gene050224	MS.gene59811	0.804344	2.26E-49	-1.69E-46
MS.gene050224	MS.gene60163	0.830082	3.59E-55	-1.69E-46
MS.gene050224	MS.gene60304	0.806383	8.45E-50	-1.69E-46
MS.gene050224	MS.gene60935	0.810235	1.28E-50	-1.69E-46
MS.gene050224	MS.gene62924	0.832828	7.57E-56	-1.69E-46
MS.gene050224	MS.gene63229	0.82302	1.73E-53	-1.69E-46
MS.gene050224	MS.gene63930	0.819745	9.88E-53	-1.69E-46
MS.gene050224	MS.gene64800	0.814966	1.18E-51	-1.69E-46
MS.gene050224	MS.gene65300	0.811333	7.38E-51	-1.69E-46
MS.gene050224	MS.gene67553	0.814822	1.27E-51	-1.69E-46
MS.gene050224	MS.gene67881	0.818978	1.48E-52	-1.69E-46
MS.gene050224	MS.gene68107	0.830893	2.27E-55	-1.69E-46
MS.gene050224	MS.gene68360	0.806596	7.62E-50	-1.69E-46
MS.gene050224	MS.gene68418	0.809579	1.77E-50	-1.69E-46
MS.gene050224	MS.gene74235	0.822991	1.76E-53	-1.69E-46
MS.gene050224	MS.gene76228	0.807195	5.69E-50	-1.69E-46
MS.gene050224	MS.gene76506	0.831034	2.10E-55	-1.69E-46
MS.gene050224	MS.gene78189	0.802661	5.05E-49	-1.69E-46
MS.gene050224	MS.gene78364	0.805703	1.17E-49	-1.69E-46
MS.gene050224	MS.gene80201	0.80485	1.77E-49	-1.69E-46
MS.gene050224	MS.gene80202	0.81013	1.34E-50	-1.69E-46
MS.gene050224	MS.gene80732	0.812802	3.53E-51	-1.69E-46
MS.gene050224	MS.gene82806	0.833322	5.70E-56	-1.69E-46
MS.gene050224	MS.gene82912	0.801259	9.79E-49	-1.69E-46
MS.gene050224	MS.gene82989	0.815295	9.94E-52	-1.69E-46
MS.gene050224	MS.gene84498	0.818973	1.48E-52	-1.69E-46
MS.gene050224	MS.gene85122	0.835062	2.09E-56	-1.69E-46
MS.gene050224	MS.gene86267	0.809922	1.49E-50	-1.69E-46
MS.gene050224	MS.gene87622	0.811487	6.83E-51	-1.69E-46
MS.gene050224	MS.gene88032	0.824586	7.45E-54	-1.69E-46
MS.gene050224	MS.gene89368	0.847056	1.47E-59	-1.69E-46
MS.gene050224	MS.gene89369	0.831577	1.54E-55	-1.69E-46
MS.gene050224	MS.gene90121	0.827706	1.35E-54	-1.69E-46
MS.gene050224	MS.gene90386	0.82048	6.71E-53	-1.69E-46
MS.gene050224	MS.gene90431	0.807333	5.32E-50	-1.69E-46
MS.gene050224	MS.gene90778	0.83163	1.50E-55	-1.69E-46
MS.gene050224	MS.gene93728	0.81865	1.75E-52	-1.69E-46
MS.gene050224	MS.gene95562	0.803286	3.75E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTGCAAAGTTGCTAATTT+TGG	0.117845	1.1:+66885533	MS.gene050224:CDS
GTTGGGTGGTCTTCTCCTTT+TGG	0.268629	1.1:-66885455	None:intergenic
AAACTTACTGTTATTGCAAT+AGG	0.309340	1.1:-66885170	None:intergenic
GTTCTTTCTGCAATTAAATC+AGG	0.315243	1.1:+66885621	MS.gene050224:CDS
ACCTTGCGAATTTGACTATT+CGG	0.317916	1.1:-66885765	None:intergenic
AAAATTAGCAACTTTGCAGC+TGG	0.442890	1.1:-66885531	None:intergenic
GCATCAACATTGAATGCAAC+TGG	0.462945	1.1:+66885570	MS.gene050224:CDS
ACCTGCTTCATCTAAAGAAA+AGG	0.464759	1.1:+66885659	MS.gene050224:CDS
CAGCAGCTCAGTAAACCAAA+AGG	0.507674	1.1:+66885440	MS.gene050224:CDS
GGTTCAAAAGAAGTGGCTGT+TGG	0.541517	1.1:+66885143	MS.gene050224:CDS
AAAGAACATCACCTTTCAGT+AGG	0.552826	1.1:-66885605	None:intergenic
CTGGTGCAACTATCTTAGCT+AGG	0.563129	1.1:-66885118	None:intergenic
ACCAGAAGGTTCAAAAGAAG+TGG	0.583999	1.1:+66885136	MS.gene050224:CDS
TTGAATGCAACTGGTCCTCA+CGG	0.601871	1.1:+66885579	MS.gene050224:CDS
AAGAACATCACCTTTCAGTA+GGG	0.605863	1.1:-66885604	None:intergenic
TCCGAATAGTCAAATTCGCA+AGG	0.610832	1.1:+66885764	MS.gene050224:CDS
GCTAAGATAGTTGCACCAGA+AGG	0.613963	1.1:+66885122	MS.gene050224:CDS
CCTCACGGCACCCTACTGAA+AGG	0.619375	1.1:+66885594	MS.gene050224:CDS
CCTTTCAGTAGGGTGCCGTG+AGG	0.734484	1.1:-66885594	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATTAACCAATAAATTTA+CGG	-	chr1.1:66885298-66885317	None:intergenic	10.0%
!	AAACTTACTGTTATTGCAAT+AGG	-	chr1.1:66885173-66885192	None:intergenic	25.0%
!	ATGATGTTCCGTAAATTTAT+TGG	+	chr1.1:66885287-66885306	MS.gene050224:intron	25.0%
!!!	AAAAGCAAAGAGTATCAATT+TGG	-	chr1.1:66885321-66885340	None:intergenic	25.0%
!!!	AAGTTGCTAATTTTGGAATA+TGG	+	chr1.1:66885540-66885559	MS.gene050224:CDS	25.0%
	GTTCTTTCTGCAATTAAATC+AGG	+	chr1.1:66885621-66885640	MS.gene050224:CDS	30.0%
!!	TTCACTTTTAGTGTCATGTT+GGG	-	chr1.1:66885475-66885494	None:intergenic	30.0%
!!!	GCTAATTTTGGAATATGGAT+TGG	+	chr1.1:66885545-66885564	MS.gene050224:CDS	30.0%
	AAAATTAGCAACTTTGCAGC+TGG	-	chr1.1:66885534-66885553	None:intergenic	35.0%
	AAAGAACATCACCTTTCAGT+AGG	-	chr1.1:66885608-66885627	None:intergenic	35.0%
	AAGAACATCACCTTTCAGTA+GGG	-	chr1.1:66885607-66885626	None:intergenic	35.0%
	ACCTGCTTCATCTAAAGAAA+AGG	+	chr1.1:66885659-66885678	MS.gene050224:CDS	35.0%
!	AGCTGCAAAGTTGCTAATTT+TGG	+	chr1.1:66885533-66885552	MS.gene050224:CDS	35.0%
!!	CTTCACTTTTAGTGTCATGT+TGG	-	chr1.1:66885476-66885495	None:intergenic	35.0%
	AAAAGTAGATGCCAGATCGT+TGG	-	chr1.1:66885244-66885263	None:intergenic	40.0%
	ACCAGAAGGTTCAAAAGAAG+TGG	+	chr1.1:66885136-66885155	MS.gene050224:CDS	40.0%
	GCATCAACATTGAATGCAAC+TGG	+	chr1.1:66885570-66885589	MS.gene050224:CDS	40.0%
	TCCGAATAGTCAAATTCGCA+AGG	+	chr1.1:66885764-66885783	MS.gene050224:CDS	40.0%
!	ACTTTTAGTGTCATGTTGGG+TGG	-	chr1.1:66885472-66885491	None:intergenic	40.0%
!	GCCTTTTCTTTAGATGAAGC+AGG	-	chr1.1:66885663-66885682	None:intergenic	40.0%
	AAGTTTGAGCTCCAACGATC+TGG	+	chr1.1:66885230-66885249	MS.gene050224:intron	45.0%
	CAGCAGCTCAGTAAACCAAA+AGG	+	chr1.1:66885440-66885459	MS.gene050224:CDS	45.0%
	CTGGTGCAACTATCTTAGCT+AGG	-	chr1.1:66885121-66885140	None:intergenic	45.0%
	GCTAAGATAGTTGCACCAGA+AGG	+	chr1.1:66885122-66885141	MS.gene050224:CDS	45.0%
	GGTTCAAAAGAAGTGGCTGT+TGG	+	chr1.1:66885143-66885162	MS.gene050224:CDS	45.0%
	TTGAATGCAACTGGTCCTCA+CGG	+	chr1.1:66885579-66885598	MS.gene050224:CDS	45.0%
!	GCCACTTCTTTTGAACCTTC+TGG	-	chr1.1:66885140-66885159	None:intergenic	45.0%
	CTTCAACCTTGCGAAAGAGC+AGG	-	chr1.1:66885383-66885402	None:intergenic	50.0%
!	GTTGGGTGGTCTTCTCCTTT+TGG	-	chr1.1:66885458-66885477	None:intergenic	50.0%
	ATGCTGCCTGCTCTTTCGCA+AGG	+	chr1.1:66885374-66885393	MS.gene050224:intron	55.0%
	CCTCACGGCACCCTACTGAA+AGG	+	chr1.1:66885594-66885613	MS.gene050224:CDS	60.0%
!!	CCTTTCAGTAGGGTGCCGTG+AGG	-	chr1.1:66885597-66885616	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	66885115	66885785	66885115	ID=MS.gene050224
chr1.1	mRNA	66885115	66885785	66885115	ID=MS.gene050224.t1;Parent=MS.gene050224
chr1.1	exon	66885115	66885184	66885115	ID=MS.gene050224.t1.exon1;Parent=MS.gene050224.t1
chr1.1	CDS	66885115	66885184	66885115	ID=cds.MS.gene050224.t1;Parent=MS.gene050224.t1
chr1.1	exon	66885396	66885785	66885396	ID=MS.gene050224.t1.exon2;Parent=MS.gene050224.t1
chr1.1	CDS	66885396	66885785	66885396	ID=cds.MS.gene050224.t1;Parent=MS.gene050224.t1

Gene Sequence

>MS.gene050224

GTACCTAGCTAAGATAGTTGCACCAGAAGGTTCAAAAGAAGTGGCTGTTGGACAGCCTATTGCAATAACAGTAAGTTTGAAAATGCTTTGTAACTTCTTCTATCAACACATTGTAAAGTTTGAGCTCCAACGATCTGGCATCTACTTTTGAAATACAAACTAAAAATGTCTGATGATGTTCCGTAAATTTATTGGTTAATTTACCAAATTGATACTCTTTGCTTTTGTCTTGAAAAGCATTGTAGTGTATGAAATAATTATGCTGCCTGCTCTTTCGCAAGGTTGAAGATGCAAGTGATATTGAAGCTGTTAAGAATTCTATTAGCAGCAGCTCAGTAAACCAAAAGGAGAAGACCACCCAACATGACACTAAAAGTGAAGTCAAAACAAAGAAAACGAACACAGCACGAATCAGTCCAGCTGCAAAGTTGCTAATTTTGGAATATGGATTGGACGCATCAACATTGAATGCAACTGGTCCTCACGGCACCCTACTGAAAGGTGATGTTCTTTCTGCAATTAAATCAGGAAAATTGTCTTCAAAACCTGCTTCATCTAAAGAAAAGGCATCATCATCTCAAAGTCATCAAGTTGCGACTTCACAAGAATCAAAGTCTAGCTTAAAGCAGTCAGATACTTATGATGATTTTCCGAATAGTCAAATTCGCAAG

Protein sequence

>MS.gene050224.t1

VPSUDSCTRRFKRSGCWTAYCNNSURCKUYUSCUEFYUQQLSKPKGEDHPTUHUKUSQNKENEHSTNQSSCKVANFGIWIGRINIECNWSSRHPTERUCSFCNUIRKIVFKTCFIURKGIIISKSSSCDFTRIKVULKAVRYLUUFSEUSNSQ