Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43433.t1 | RHN65115.1 | 96.7 | 330 | 11 | 0 | 1 | 330 | 1 | 330 | 1.60E-184 | 655.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43433.t1 | P46250 | 30.5 | 213 | 129 | 5 | 33 | 234 | 57 | 261 | 8.4e-17 | 89.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43433.t1 | A0A396IML6 | 96.7 | 330 | 11 | 0 | 1 | 330 | 1 | 330 | 1.2e-184 | 655.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050381 | MS.gene43433 | 0.825922 | 3.60E-54 | -1.69E-46 |
| MS.gene053561 | MS.gene43433 | 0.803745 | 3.01E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene43433 | 0.819392 | 1.19E-52 | -1.69E-46 |
| MS.gene056313 | MS.gene43433 | 0.838642 | 2.55E-57 | -1.69E-46 |
| MS.gene059697 | MS.gene43433 | 0.813462 | 2.53E-51 | -1.69E-46 |
| MS.gene059943 | MS.gene43433 | 0.811443 | 6.98E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43433.t1 | MTR_2g027140 | 81.553 | 309 | 57 | 0 | 2 | 310 | 7 | 315 | 0.0 | 514 |
| MS.gene43433.t1 | MTR_3g060670 | 66.978 | 321 | 102 | 3 | 1 | 318 | 1 | 320 | 2.97e-164 | 462 |
| MS.gene43433.t1 | MTR_2g027140 | 84.231 | 260 | 41 | 0 | 51 | 310 | 1 | 260 | 1.56e-157 | 442 |
| MS.gene43433.t1 | MTR_1099s0010 | 96.970 | 165 | 5 | 0 | 166 | 330 | 1 | 165 | 1.32e-119 | 341 |
| MS.gene43433.t1 | MTR_4g133440 | 95.783 | 166 | 7 | 0 | 1 | 166 | 1 | 166 | 1.95e-115 | 331 |
| MS.gene43433.t1 | MTR_4g134120 | 95.783 | 166 | 7 | 0 | 1 | 166 | 1 | 166 | 1.95e-115 | 331 |
| MS.gene43433.t1 | MTR_7g056587 | 29.603 | 277 | 165 | 9 | 26 | 290 | 103 | 361 | 2.49e-21 | 95.1 |
| MS.gene43433.t1 | MTR_7g056587 | 29.603 | 277 | 165 | 9 | 26 | 290 | 117 | 375 | 2.73e-21 | 95.1 |
| MS.gene43433.t1 | MTR_4g070720 | 29.437 | 231 | 151 | 4 | 34 | 254 | 97 | 325 | 2.89e-21 | 94.7 |
| MS.gene43433.t1 | MTR_7g056587 | 29.603 | 277 | 165 | 9 | 26 | 290 | 95 | 353 | 2.94e-21 | 94.7 |
| MS.gene43433.t1 | MTR_7g056587 | 29.603 | 277 | 165 | 9 | 26 | 290 | 103 | 361 | 3.15e-21 | 94.7 |
| MS.gene43433.t1 | MTR_3g117090 | 29.870 | 231 | 150 | 4 | 34 | 254 | 98 | 326 | 9.39e-21 | 93.2 |
| MS.gene43433.t1 | MTR_3g117090 | 29.870 | 231 | 150 | 4 | 34 | 254 | 98 | 326 | 9.64e-21 | 93.2 |
| MS.gene43433.t1 | MTR_3g117100 | 28.627 | 255 | 153 | 7 | 8 | 251 | 93 | 329 | 1.97e-19 | 89.4 |
| MS.gene43433.t1 | MTR_8g071000 | 29.658 | 263 | 160 | 7 | 26 | 272 | 85 | 338 | 2.00e-19 | 89.4 |
| MS.gene43433.t1 | MTR_5g012460 | 29.493 | 217 | 143 | 4 | 26 | 236 | 110 | 322 | 5.18e-19 | 87.8 |
| MS.gene43433.t1 | MTR_3g112150 | 28.364 | 275 | 159 | 10 | 1 | 260 | 59 | 310 | 8.97e-19 | 87.4 |
| MS.gene43433.t1 | MTR_4g070810 | 28.708 | 209 | 141 | 3 | 34 | 236 | 108 | 314 | 1.91e-18 | 86.7 |
| MS.gene43433.t1 | MTR_8g076880 | 29.741 | 232 | 142 | 7 | 34 | 250 | 80 | 305 | 6.86e-18 | 84.7 |
| MS.gene43433.t1 | MTR_1g022480 | 30.047 | 213 | 133 | 4 | 34 | 236 | 113 | 319 | 7.09e-18 | 84.7 |
| MS.gene43433.t1 | MTR_1g022480 | 30.047 | 213 | 133 | 4 | 34 | 236 | 113 | 319 | 7.32e-18 | 84.7 |
| MS.gene43433.t1 | MTR_1g022480 | 30.047 | 213 | 133 | 4 | 34 | 236 | 113 | 319 | 7.83e-18 | 84.7 |
| MS.gene43433.t1 | MTR_5g021050 | 28.326 | 233 | 145 | 6 | 34 | 251 | 77 | 302 | 8.54e-18 | 84.3 |
| MS.gene43433.t1 | MTR_5g021050 | 29.060 | 234 | 141 | 7 | 34 | 250 | 77 | 302 | 1.02e-17 | 84.0 |
| MS.gene43433.t1 | MTR_1g022480 | 29.437 | 231 | 140 | 6 | 36 | 250 | 31 | 254 | 1.92e-17 | 83.6 |
| MS.gene43433.t1 | MTR_7g056587 | 28.175 | 252 | 153 | 8 | 51 | 290 | 1 | 236 | 8.37e-16 | 78.2 |
| MS.gene43433.t1 | MTR_3g010190 | 27.897 | 233 | 154 | 6 | 26 | 251 | 97 | 322 | 1.66e-15 | 77.4 |
| MS.gene43433.t1 | MTR_6g015645 | 30.508 | 177 | 113 | 3 | 88 | 254 | 25 | 201 | 8.15e-15 | 75.1 |
| MS.gene43433.t1 | MTR_1g032580 | 26.923 | 208 | 124 | 8 | 27 | 225 | 25 | 213 | 7.78e-13 | 67.8 |
| MS.gene43433.t1 | MTR_8g096180 | 25.592 | 211 | 136 | 5 | 34 | 235 | 50 | 248 | 2.56e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43433.t1 | AT1G55840 | 64.596 | 322 | 110 | 2 | 2 | 322 | 7 | 325 | 4.89e-165 | 462 |
| MS.gene43433.t1 | AT5G47730 | 70.550 | 309 | 89 | 1 | 2 | 310 | 7 | 313 | 5.29e-165 | 463 |
| MS.gene43433.t1 | AT5G47730 | 70.550 | 309 | 89 | 1 | 2 | 310 | 7 | 313 | 5.29e-165 | 463 |
| MS.gene43433.t1 | AT1G55840 | 67.333 | 300 | 94 | 2 | 24 | 322 | 2 | 298 | 1.34e-159 | 448 |
| MS.gene43433.t1 | AT5G47730 | 74.231 | 260 | 65 | 1 | 51 | 310 | 1 | 258 | 1.52e-142 | 404 |
| MS.gene43433.t1 | AT5G47730 | 74.231 | 260 | 65 | 1 | 51 | 310 | 1 | 258 | 1.52e-142 | 404 |
| MS.gene43433.t1 | AT4G39180 | 29.195 | 298 | 176 | 9 | 34 | 310 | 97 | 380 | 4.78e-23 | 100 |
| MS.gene43433.t1 | AT4G39180 | 29.195 | 298 | 176 | 9 | 34 | 310 | 97 | 380 | 4.78e-23 | 100 |
| MS.gene43433.t1 | AT4G39180 | 32.340 | 235 | 139 | 5 | 34 | 254 | 97 | 325 | 1.00e-22 | 99.0 |
| MS.gene43433.t1 | AT3G24840 | 30.380 | 237 | 148 | 6 | 26 | 250 | 98 | 329 | 9.75e-22 | 96.3 |
| MS.gene43433.t1 | AT3G24840 | 30.380 | 237 | 148 | 6 | 26 | 250 | 98 | 329 | 9.75e-22 | 96.3 |
| MS.gene43433.t1 | AT3G24840 | 30.380 | 237 | 148 | 6 | 26 | 250 | 98 | 329 | 9.75e-22 | 96.3 |
| MS.gene43433.t1 | AT3G24840 | 30.380 | 237 | 148 | 6 | 26 | 250 | 98 | 329 | 1.08e-21 | 96.3 |
| MS.gene43433.t1 | AT3G24840 | 30.380 | 237 | 148 | 6 | 26 | 250 | 98 | 329 | 1.08e-21 | 96.3 |
| MS.gene43433.t1 | AT3G24840 | 30.380 | 237 | 148 | 6 | 26 | 250 | 98 | 329 | 1.08e-21 | 96.3 |
| MS.gene43433.t1 | AT3G24840 | 30.380 | 237 | 148 | 6 | 26 | 250 | 98 | 329 | 1.08e-21 | 96.3 |
| MS.gene43433.t1 | AT4G34580 | 28.685 | 251 | 161 | 5 | 14 | 254 | 75 | 317 | 3.29e-21 | 94.7 |
| MS.gene43433.t1 | AT4G34580 | 28.685 | 251 | 161 | 5 | 14 | 254 | 75 | 317 | 3.29e-21 | 94.7 |
| MS.gene43433.t1 | AT2G21540 | 30.213 | 235 | 144 | 5 | 34 | 254 | 96 | 324 | 6.53e-21 | 94.0 |
| MS.gene43433.t1 | AT2G21540 | 30.213 | 235 | 144 | 5 | 34 | 254 | 96 | 324 | 6.53e-21 | 94.0 |
| MS.gene43433.t1 | AT5G47510 | 30.043 | 233 | 141 | 6 | 34 | 260 | 52 | 268 | 6.72e-21 | 92.4 |
| MS.gene43433.t1 | AT2G21540 | 30.213 | 235 | 144 | 5 | 34 | 254 | 96 | 324 | 7.41e-21 | 93.6 |
| MS.gene43433.t1 | AT2G21540 | 30.213 | 235 | 144 | 5 | 34 | 254 | 96 | 324 | 7.41e-21 | 93.6 |
| MS.gene43433.t1 | AT2G21540 | 30.213 | 235 | 144 | 5 | 34 | 254 | 96 | 324 | 7.41e-21 | 93.6 |
| MS.gene43433.t1 | AT2G21540 | 30.213 | 235 | 144 | 5 | 34 | 254 | 96 | 324 | 7.41e-21 | 93.6 |
| MS.gene43433.t1 | AT5G47510 | 30.043 | 233 | 141 | 6 | 34 | 260 | 52 | 268 | 8.18e-21 | 92.4 |
| MS.gene43433.t1 | AT5G47510 | 30.043 | 233 | 141 | 6 | 34 | 260 | 51 | 267 | 8.18e-21 | 92.4 |
| MS.gene43433.t1 | AT4G36490 | 30.172 | 232 | 142 | 6 | 34 | 251 | 79 | 304 | 5.04e-20 | 91.3 |
| MS.gene43433.t1 | AT2G18180 | 30.213 | 235 | 144 | 5 | 34 | 254 | 82 | 310 | 5.45e-20 | 90.9 |
| MS.gene43433.t1 | AT2G18180 | 30.472 | 233 | 142 | 5 | 36 | 254 | 13 | 239 | 6.15e-20 | 90.9 |
| MS.gene43433.t1 | AT2G18180 | 30.472 | 233 | 142 | 5 | 36 | 254 | 13 | 239 | 6.20e-20 | 90.5 |
| MS.gene43433.t1 | AT2G18180 | 30.213 | 235 | 144 | 5 | 34 | 254 | 82 | 310 | 6.31e-20 | 90.9 |
| MS.gene43433.t1 | AT2G16380 | 29.362 | 235 | 146 | 5 | 34 | 254 | 89 | 317 | 6.91e-20 | 90.9 |
| MS.gene43433.t1 | AT2G16380 | 29.362 | 235 | 146 | 5 | 34 | 254 | 89 | 317 | 6.91e-20 | 90.9 |
| MS.gene43433.t1 | AT2G21520 | 29.464 | 224 | 147 | 4 | 34 | 251 | 115 | 333 | 1.22e-19 | 90.1 |
| MS.gene43433.t1 | AT2G21520 | 29.464 | 224 | 147 | 4 | 34 | 251 | 115 | 333 | 1.22e-19 | 90.1 |
| MS.gene43433.t1 | AT2G21520 | 29.464 | 224 | 147 | 4 | 34 | 251 | 111 | 329 | 1.24e-19 | 90.1 |
| MS.gene43433.t1 | AT1G19650 | 28.309 | 272 | 157 | 8 | 14 | 250 | 62 | 330 | 2.43e-19 | 88.6 |
| MS.gene43433.t1 | AT1G19650 | 28.309 | 272 | 157 | 8 | 14 | 250 | 62 | 330 | 3.67e-19 | 88.6 |
| MS.gene43433.t1 | AT1G19650 | 30.131 | 229 | 148 | 5 | 33 | 251 | 32 | 258 | 7.43e-19 | 87.8 |
| MS.gene43433.t1 | AT4G39170 | 28.947 | 228 | 143 | 5 | 34 | 251 | 109 | 327 | 1.29e-18 | 87.0 |
| MS.gene43433.t1 | AT4G39170 | 28.947 | 228 | 143 | 5 | 34 | 251 | 109 | 327 | 1.39e-18 | 87.0 |
| MS.gene43433.t1 | AT5G47510 | 28.761 | 226 | 139 | 6 | 41 | 260 | 2 | 211 | 1.15e-17 | 82.8 |
| MS.gene43433.t1 | AT1G75370 | 27.273 | 231 | 156 | 4 | 34 | 254 | 114 | 342 | 1.22e-16 | 81.3 |
| MS.gene43433.t1 | AT1G75370 | 27.273 | 231 | 156 | 4 | 34 | 254 | 114 | 342 | 1.33e-16 | 80.9 |
| MS.gene43433.t1 | AT5G56160 | 27.338 | 278 | 185 | 8 | 8 | 272 | 69 | 342 | 7.34e-16 | 78.6 |
| MS.gene43433.t1 | AT5G56160 | 27.338 | 278 | 185 | 8 | 8 | 272 | 69 | 342 | 8.10e-16 | 78.6 |
| MS.gene43433.t1 | AT5G56160 | 27.338 | 278 | 185 | 8 | 8 | 272 | 69 | 342 | 8.10e-16 | 78.6 |
| MS.gene43433.t1 | AT1G55690 | 28.571 | 238 | 153 | 6 | 26 | 250 | 96 | 329 | 1.94e-15 | 77.4 |
| MS.gene43433.t1 | AT1G55690 | 28.571 | 238 | 153 | 6 | 26 | 250 | 96 | 329 | 1.94e-15 | 77.4 |
| MS.gene43433.t1 | AT1G55690 | 28.571 | 238 | 153 | 6 | 26 | 250 | 96 | 329 | 1.94e-15 | 77.4 |
| MS.gene43433.t1 | AT1G55690 | 28.571 | 238 | 153 | 6 | 26 | 250 | 96 | 329 | 1.94e-15 | 77.4 |
| MS.gene43433.t1 | AT1G55690 | 28.571 | 238 | 153 | 6 | 26 | 250 | 96 | 329 | 1.95e-15 | 77.4 |
| MS.gene43433.t1 | AT2G18180 | 28.440 | 218 | 136 | 5 | 51 | 254 | 1 | 212 | 1.38e-14 | 74.7 |
| MS.gene43433.t1 | AT2G16380 | 27.523 | 218 | 138 | 5 | 51 | 254 | 1 | 212 | 5.71e-14 | 72.8 |
| MS.gene43433.t1 | AT1G01630 | 27.322 | 183 | 113 | 7 | 34 | 213 | 53 | 218 | 1.04e-11 | 64.7 |
Find 77 sgRNAs with CRISPR-Local
Find 190 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAAATCAGCAGGAATAAT+TGG | 0.243946 | 8.4:-718856 | None:intergenic |
| CCTAACCTTGAATGGAAATA+AGG | 0.294757 | 8.4:+721543 | MS.gene43433:CDS |
| GTATCCACATCCGGAGGTTC+TGG | 0.320907 | 8.4:-721484 | None:intergenic |
| AAGGGATTGGGATCCTTCTA+AGG | 0.324848 | 8.4:+718590 | MS.gene43433:CDS |
| GGTCTTCCTGTCTTTGCAAT+TGG | 0.327042 | 8.4:+719032 | MS.gene43433:CDS |
| TAATTCCTGCAACTGATTCA+TGG | 0.344567 | 8.4:-718378 | None:intergenic |
| AGAAAAGTGCAGGTCTTATC+AGG | 0.347127 | 8.4:+719993 | MS.gene43433:CDS |
| AGTTGATTGATTGTTTAAAC+TGG | 0.349882 | 8.4:+718711 | MS.gene43433:intron |
| GATTCAAGGCCGAGAGCTTC+AGG | 0.351238 | 8.4:-719396 | None:intergenic |
| CTCGGCCTTGAATCACATTA+AGG | 0.354278 | 8.4:+719405 | MS.gene43433:CDS |
| TGCAACAGCTTCACGGAGTC+TGG | 0.368534 | 8.4:-721428 | None:intergenic |
| TTGTTCCTTATTTCCATTCA+AGG | 0.371242 | 8.4:-721548 | None:intergenic |
| CCTGTCTTTGCAATTGGTGT+TGG | 0.372437 | 8.4:+719038 | MS.gene43433:CDS |
| ACCATTTGATAAGCCTTAGA+AGG | 0.373448 | 8.4:-718603 | None:intergenic |
| CAACAAGCTGAAAATATGTA+TGG | 0.379181 | 8.4:-719794 | None:intergenic |
| ATAAGGAACAATTGAACAAT+TGG | 0.379476 | 8.4:+721560 | MS.gene43433:CDS |
| CCTTATTTCCATTCAAGGTT+AGG | 0.386830 | 8.4:-721543 | None:intergenic |
| ACCTTTACACAAGTGGTAAT+AGG | 0.387066 | 8.4:-719358 | None:intergenic |
| TTTGATAGCCTAACCTTGAA+TGG | 0.388252 | 8.4:+721535 | MS.gene43433:CDS |
| TCACAACTCATTGGATTGTC+CGG | 0.400882 | 8.4:+718895 | MS.gene43433:CDS |
| GCTTCCACCTTCTGAATGTC+TGG | 0.409269 | 8.4:-721341 | None:intergenic |
| TGTGAGACTGCACATAATAA+TGG | 0.425984 | 8.4:-719183 | None:intergenic |
| GCTTTCTAAAAGCAAGGGAT+TGG | 0.428984 | 8.4:+718577 | MS.gene43433:CDS |
| CTTTCTAAAAGCAAGGGATT+GGG | 0.432846 | 8.4:+718578 | MS.gene43433:CDS |
| GGATTCCATGAATCAGTTGC+AGG | 0.433771 | 8.4:+718373 | MS.gene43433:CDS |
| ATACGTGATTCACAACTCAT+TGG | 0.449153 | 8.4:+718886 | MS.gene43433:CDS |
| GCCTATTACCACTTGTGTAA+AGG | 0.454247 | 8.4:+719357 | MS.gene43433:CDS |
| TTCTGTAGAAAAGAAGGCTC+TGG | 0.457102 | 8.4:+721313 | MS.gene43433:CDS |
| ATGAAATGATCCCTGCTTGA+TGG | 0.479907 | 8.4:-721452 | None:intergenic |
| CCACATTTCTGTAGAAAAGA+AGG | 0.481927 | 8.4:+721307 | MS.gene43433:CDS |
| AGGTACCTTAATGTGATTCA+AGG | 0.487226 | 8.4:-719410 | None:intergenic |
| TCCTTCTAAGGCTTATCAAA+TGG | 0.487749 | 8.4:+718602 | MS.gene43433:CDS |
| CCTGAAAAGTTGTCTGCAAT+GGG | 0.500033 | 8.4:-718418 | None:intergenic |
| ATATCGCGATCGTGTAATCT+TGG | 0.500716 | 8.4:+719219 | MS.gene43433:CDS |
| ACCTGAAAAGTTGTCTGCAA+TGG | 0.500831 | 8.4:-718419 | None:intergenic |
| GTCTAAAACCTTTACACAAG+TGG | 0.503259 | 8.4:-719365 | None:intergenic |
| AGCTGTTGCACCCATCAAGC+AGG | 0.504047 | 8.4:+721441 | MS.gene43433:CDS |
| TGTGGAATTTCCAGAACCTC+CGG | 0.505738 | 8.4:+721474 | MS.gene43433:CDS |
| CTTGTCCTCTCGTGTAAAAG+TGG | 0.505908 | 8.4:-719969 | None:intergenic |
| AAGCGAGAACTGCTATTCCT+TGG | 0.507986 | 8.4:+721360 | MS.gene43433:CDS |
| AAATAAGTATGAGTCTTCTC+AGG | 0.511983 | 8.4:-719758 | None:intergenic |
| GCAACCCTTGTATCCACATC+CGG | 0.513462 | 8.4:-721493 | None:intergenic |
| ATTTCCAGAACCTCCGGATG+TGG | 0.516108 | 8.4:+721480 | MS.gene43433:CDS |
| TGATGGGTGCAACAGCTTCA+CGG | 0.519474 | 8.4:-721435 | None:intergenic |
| AGGATAGTTGAGATCATCAA+TGG | 0.519931 | 8.4:-719738 | None:intergenic |
| CACAACTCATTGGATTGTCC+GGG | 0.520676 | 8.4:+718896 | MS.gene43433:CDS |
| AACCTCCGGATGTGGATACA+AGG | 0.521017 | 8.4:+721488 | MS.gene43433:CDS |
| AACAAGCTGAAAATATGTAT+GGG | 0.528848 | 8.4:-719793 | None:intergenic |
| ATTGTTCTATATAAATCAGC+AGG | 0.538192 | 8.4:-718865 | None:intergenic |
| GACTCGCTTTCTAAAAGCAA+GGG | 0.549883 | 8.4:+718572 | MS.gene43433:CDS |
| CCAACACCAATTGCAAAGAC+AGG | 0.557244 | 8.4:-719038 | None:intergenic |
| GAGGACAAGAAGAAAAGTGC+AGG | 0.566912 | 8.4:+719983 | MS.gene43433:CDS |
| CCATGCTTCTTCGATGCAGA+AGG | 0.568603 | 8.4:-719331 | None:intergenic |
| AATGATCAGAATGTTCATCA+AGG | 0.572623 | 8.4:+718531 | MS.gene43433:intron |
| TTGTAAAGCTGTTGGTGAAG+GGG | 0.576838 | 8.4:-721388 | None:intergenic |
| GAACAATTGGACTAGAGCAC+AGG | 0.579823 | 8.4:+721573 | MS.gene43433:CDS |
| CATGACTGGCCTGAAGCTCT+CGG | 0.583248 | 8.4:+719387 | MS.gene43433:CDS |
| TTGGTGAAGGGGATGATCCA+AGG | 0.586370 | 8.4:-721377 | None:intergenic |
| GTGCAGGTCTTATCAGGATG+CGG | 0.586473 | 8.4:+719999 | MS.gene43433:CDS |
| TGAAATGATCCCTGCTTGAT+GGG | 0.594800 | 8.4:-721451 | None:intergenic |
| AGTTGTAAAGCTGTTGGTGA+AGG | 0.599771 | 8.4:-721390 | None:intergenic |
| GAGCACCTCTCTTGTATACC+CGG | 0.601263 | 8.4:-718914 | None:intergenic |
| CCTTCTGCATCGAAGAAGCA+TGG | 0.604202 | 8.4:+719331 | MS.gene43433:CDS |
| TGACTCGCTTTCTAAAAGCA+AGG | 0.605557 | 8.4:+718571 | MS.gene43433:CDS |
| TGATGTAGTTGTAAAGCTGT+TGG | 0.607103 | 8.4:-721396 | None:intergenic |
| GTTGTAAAGCTGTTGGTGAA+GGG | 0.611186 | 8.4:-721389 | None:intergenic |
| GCTGTTGCACCCATCAAGCA+GGG | 0.617758 | 8.4:+721442 | MS.gene43433:CDS |
| TCATCCAGACATTCAGAAGG+TGG | 0.620255 | 8.4:+721337 | MS.gene43433:CDS |
| CTTATCAGGATGCGGACGAG+AGG | 0.624579 | 8.4:+720007 | MS.gene43433:CDS |
| CTGCATCGAAGAAGCATGGA+CGG | 0.630026 | 8.4:+719335 | MS.gene43433:CDS |
| CAAGCAGGGATCATTTCATG+TGG | 0.642715 | 8.4:+721456 | MS.gene43433:CDS |
| ACAAGCTGAAAATATGTATG+GGG | 0.656374 | 8.4:-719792 | None:intergenic |
| GGATCATCCAGACATTCAGA+AGG | 0.659467 | 8.4:+721334 | MS.gene43433:CDS |
| ACCTCCGGATGTGGATACAA+GGG | 0.663935 | 8.4:+721489 | MS.gene43433:CDS |
| ATTGTCCGGGTATACAAGAG+AGG | 0.683235 | 8.4:+718909 | MS.gene43433:CDS |
| ACCCTTGTATCCACATCCGG+AGG | 0.690874 | 8.4:-721490 | None:intergenic |
| GAAAAGTTGTCTGCAATGGG+TGG | 0.699381 | 8.4:-718415 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTAAGATATATATTTA+CGG | - | chr8.4:720770-720789 | None:intergenic | 10.0% |
| !! | GAAGTATAATTAATATTAAT+AGG | - | chr8.4:720849-720868 | None:intergenic | 10.0% |
| !! | TGAATTTAACAAATTAAATT+TGG | + | chr8.4:721256-721275 | MS.gene43433:intron | 10.0% |
| !!! | TTTTTATTTAATAATAGCAT+TGG | + | chr8.4:719890-719909 | MS.gene43433:intron | 10.0% |
| !! | AAAAATGAGATCATTATAAA+CGG | - | chr8.4:720150-720169 | None:intergenic | 15.0% |
| !! | AAACTTATAAAAAAATAGAG+GGG | - | chr8.4:718812-718831 | None:intergenic | 15.0% |
| !! | AGAAACTTATAAAAAAATAG+AGG | - | chr8.4:718814-718833 | None:intergenic | 15.0% |
| !! | GAAACTTATAAAAAAATAGA+GGG | - | chr8.4:718813-718832 | None:intergenic | 15.0% |
| !! | TACAGTAAATTAAATGTTAA+GGG | - | chr8.4:720343-720362 | None:intergenic | 15.0% |
| !! | TTCAAATCAACTAAAAAATA+AGG | - | chr8.4:720733-720752 | None:intergenic | 15.0% |
| !!! | AACATCTCTTTTTATTTTTT+TGG | - | chr8.4:719841-719860 | None:intergenic | 15.0% |
| !!! | AGTTTTTTTTTTTTTTTGAC+AGG | - | chr8.4:720656-720675 | None:intergenic | 15.0% |
| !!! | GACATTTTTCATAAAATTTT+TGG | + | chr8.4:720683-720702 | MS.gene43433:intron | 15.0% |
| !!! | TTTATAATGATCTCATTTTT+TGG | + | chr8.4:720150-720169 | MS.gene43433:intron | 15.0% |
| !! | AAACATACAAATATGACATA+AGG | - | chr8.4:719157-719176 | None:intergenic | 20.0% |
| !! | AAAGAAATACAAGAACTTTA+TGG | - | chr8.4:720306-720325 | None:intergenic | 20.0% |
| !! | AATAAATACACTTATAGGAA+TGG | - | chr8.4:721114-721133 | None:intergenic | 20.0% |
| !! | ATTCATGAAGCAATAAATTA+AGG | - | chr8.4:719636-719655 | None:intergenic | 20.0% |
| !! | ATTCCTATAAGTGTATTTAT+TGG | + | chr8.4:721113-721132 | MS.gene43433:intron | 20.0% |
| !! | GAACAAAAAACTCAAATAAT+AGG | - | chr8.4:719280-719299 | None:intergenic | 20.0% |
| !! | GTACAGTAAATTAAATGTTA+AGG | - | chr8.4:720344-720363 | None:intergenic | 20.0% |
| !! | TTCATGAAGCAATAAATTAA+GGG | - | chr8.4:719635-719654 | None:intergenic | 20.0% |
| !! | TTTCATAAACAGATTTGAAT+GGG | - | chr8.4:721072-721091 | None:intergenic | 20.0% |
| !!! | ACATTTAATTTACTGTACAT+TGG | + | chr8.4:720346-720365 | MS.gene43433:intron | 20.0% |
| !!! | TTTTCATAAACAGATTTGAA+TGG | - | chr8.4:721073-721092 | None:intergenic | 20.0% |
| !!! | TTTTTCATAAAATTTTTGGC+TGG | + | chr8.4:720687-720706 | MS.gene43433:intron | 20.0% |
| ! | AACAAGCTGAAAATATGTAT+GGG | - | chr8.4:719796-719815 | None:intergenic | 25.0% |
| ! | AGTTGATTGATTGTTTAAAC+TGG | + | chr8.4:718711-718730 | MS.gene43433:intron | 25.0% |
| ! | ATAAGGAACAATTGAACAAT+TGG | + | chr8.4:721560-721579 | MS.gene43433:CDS | 25.0% |
| ! | ATACAAATATGACATAAGGA+AGG | - | chr8.4:719153-719172 | None:intergenic | 25.0% |
| ! | ATTGTTCTATATAAATCAGC+AGG | - | chr8.4:718868-718887 | None:intergenic | 25.0% |
| ! | GAACCAATAAATACACTTAT+AGG | - | chr8.4:721119-721138 | None:intergenic | 25.0% |
| ! | GTTAAGATATATATTTACGG+AGG | - | chr8.4:720767-720786 | None:intergenic | 25.0% |
| ! | TAAACAACTATTCAAGAATC+TGG | - | chr8.4:719468-719487 | None:intergenic | 25.0% |
| ! | TATAAATCAGCAGGAATAAT+TGG | - | chr8.4:718859-718878 | None:intergenic | 25.0% |
| ! | TCAAATCTGTTTATGAAAAG+CGG | + | chr8.4:721074-721093 | MS.gene43433:intron | 25.0% |
| ! | TGTTAACACTATACATTAAC+AGG | + | chr8.4:719938-719957 | MS.gene43433:intron | 25.0% |
| ! | TTCATAAACAGATTTGAATG+GGG | - | chr8.4:721071-721090 | None:intergenic | 25.0% |
| !! | ATGCAATTATTACGATCTTT+TGG | - | chr8.4:720187-720206 | None:intergenic | 25.0% |
| !! | TACTTGATCTTTTTAATCAG+TGG | - | chr8.4:721022-721041 | None:intergenic | 25.0% |
| !! | TAGTTTTCAACATATACGTT+TGG | + | chr8.4:720522-720541 | MS.gene43433:intron | 25.0% |
| !! | TGTCATATTTGTATGTTTTC+AGG | + | chr8.4:719159-719178 | MS.gene43433:intron | 25.0% |
| !! | TTGCAATAAGATTAGTTGAA+TGG | - | chr8.4:720615-720634 | None:intergenic | 25.0% |
| !!! | AAATTTTGACTCTATAGTCT+TGG | - | chr8.4:721518-721537 | None:intergenic | 25.0% |
| AAAAGAGATGTTGTTCCTTA+TGG | + | chr8.4:719849-719868 | MS.gene43433:intron | 30.0% | |
| AAATAAGTATGAGTCTTCTC+AGG | - | chr8.4:719761-719780 | None:intergenic | 30.0% | |
| AATAGAGGGGATAAATGTAA+AGG | - | chr8.4:718799-718818 | None:intergenic | 30.0% | |
| AATGATCAGAATGTTCATCA+AGG | + | chr8.4:718531-718550 | MS.gene43433:intron | 30.0% | |
| ACAAGCTGAAAATATGTATG+GGG | - | chr8.4:719795-719814 | None:intergenic | 30.0% | |
| ACAGAAGATAGAGTAATAGA+TGG | - | chr8.4:719130-719149 | None:intergenic | 30.0% | |
| ACATAGAAAACTTGTGTTAC+AGG | - | chr8.4:720573-720592 | None:intergenic | 30.0% | |
| AGATCGTAATAATTGCATAC+TGG | + | chr8.4:720189-720208 | MS.gene43433:intron | 30.0% | |
| AGTAACTAATAGACTTGTCT+TGG | - | chr8.4:720441-720460 | None:intergenic | 30.0% | |
| AGTCAATAATTGAGATGTGA+AGG | + | chr8.4:720103-720122 | MS.gene43433:intron | 30.0% | |
| ATAAATGTAAAGGATGAAGG+AGG | - | chr8.4:718789-718808 | None:intergenic | 30.0% | |
| ATAATTGCATACTGGATAAG+AGG | + | chr8.4:720197-720216 | MS.gene43433:intron | 30.0% | |
| ATGTTGAAAAGATCATTAGC+AGG | + | chr8.4:719515-719534 | MS.gene43433:intron | 30.0% | |
| CAACAAGCTGAAAATATGTA+TGG | - | chr8.4:719797-719816 | None:intergenic | 30.0% | |
| CAACACCTTGATTAAACTTA+CGG | - | chr8.4:719087-719106 | None:intergenic | 30.0% | |
| CGAGAATATACATAAGGTAA+GGG | - | chr8.4:718685-718704 | None:intergenic | 30.0% | |
| GAGATTCGAGAATATACATA+AGG | - | chr8.4:718691-718710 | None:intergenic | 30.0% | |
| GCACTTTACAAAGTAAAGTA+CGG | + | chr8.4:720988-721007 | MS.gene43433:intron | 30.0% | |
| GTAAAGATAAAAGACCTTTG+TGG | + | chr8.4:720949-720968 | MS.gene43433:intron | 30.0% | |
| GTTGAAAACTATTCCAATTC+TGG | - | chr8.4:720513-720532 | None:intergenic | 30.0% | |
| TCGAGAATATACATAAGGTA+AGG | - | chr8.4:718686-718705 | None:intergenic | 30.0% | |
| TGAAAAGGAAAGCAAAATGA+AGG | - | chr8.4:719011-719030 | None:intergenic | 30.0% | |
| TGAAACTAGTTGTTGGAAAA+TGG | + | chr8.4:720406-720425 | MS.gene43433:intron | 30.0% | |
| TGACTTAGTTAGTAGTCAAT+TGG | + | chr8.4:718978-718997 | MS.gene43433:intron | 30.0% | |
| TTTGAATGGGGCAATTTAAA+AGG | - | chr8.4:721059-721078 | None:intergenic | 30.0% | |
| ! | CATACATATTTTCAGCTTGT+TGG | + | chr8.4:719795-719814 | MS.gene43433:CDS | 30.0% |
| ! | TATTTCTACACTTTCAACTG+CGG | - | chr8.4:720475-720494 | None:intergenic | 30.0% |
| ! | TCATTTTGCTTTCCTTTTCA+GGG | + | chr8.4:719011-719030 | MS.gene43433:intron | 30.0% |
| ! | TTCATTTTGCTTTCCTTTTC+AGG | + | chr8.4:719010-719029 | MS.gene43433:intron | 30.0% |
| ! | TTGTTCCTTATTTCCATTCA+AGG | - | chr8.4:721551-721570 | None:intergenic | 30.0% |
| ! | TTTCCAACAACTAGTTTCAA+AGG | - | chr8.4:720405-720424 | None:intergenic | 30.0% |
| !! | AATCTTGCTCTAAGAAACAA+AGG | - | chr8.4:720069-720088 | None:intergenic | 30.0% |
| !! | TAAATTTGGTGTGTAGATCA+TGG | + | chr8.4:721270-721289 | MS.gene43433:intron | 30.0% |
| AAGTAGCAGTGGAAACTAAA+AGG | - | chr8.4:719433-719452 | None:intergenic | 35.0% | |
| ACCATTTGATAAGCCTTAGA+AGG | - | chr8.4:718606-718625 | None:intergenic | 35.0% | |
| ACCTTTACACAAGTGGTAAT+AGG | - | chr8.4:719361-719380 | None:intergenic | 35.0% | |
| AGCATCCGTAAGTTTAATCA+AGG | + | chr8.4:719079-719098 | MS.gene43433:intron | 35.0% | |
| AGGATAGTTGAGATCATCAA+TGG | - | chr8.4:719741-719760 | None:intergenic | 35.0% | |
| AGGTACCTTAATGTGATTCA+AGG | - | chr8.4:719413-719432 | None:intergenic | 35.0% | |
| ATACGTGATTCACAACTCAT+TGG | + | chr8.4:718886-718905 | MS.gene43433:CDS | 35.0% | |
| ATAGTGTTAACATCTTGCTG+AGG | - | chr8.4:719930-719949 | None:intergenic | 35.0% | |
| ATTCAAGAATCTGGTCCTAT+TGG | - | chr8.4:719459-719478 | None:intergenic | 35.0% | |
| CCACATTTCTGTAGAAAAGA+AGG | + | chr8.4:721307-721326 | MS.gene43433:CDS | 35.0% | |
| CCTAACCTTGAATGGAAATA+AGG | + | chr8.4:721543-721562 | MS.gene43433:CDS | 35.0% | |
| GGGATAAATGTAAAGGATGA+AGG | - | chr8.4:718792-718811 | None:intergenic | 35.0% | |
| TCAGATGCACTACTGTTAAT+TGG | - | chr8.4:719547-719566 | None:intergenic | 35.0% | |
| TGATGTAGTTGTAAAGCTGT+TGG | - | chr8.4:721399-721418 | None:intergenic | 35.0% | |
| TGCACTACTGTTAATTGGTT+CGG | - | chr8.4:719542-719561 | None:intergenic | 35.0% | |
| TGTGAGACTGCACATAATAA+TGG | - | chr8.4:719186-719205 | None:intergenic | 35.0% | |
| ! | AAATGTTAAGGGCATGTATG+TGG | - | chr8.4:720332-720351 | None:intergenic | 35.0% |
| ! | AACTAGTTTCAAAGGCTTTG+TGG | - | chr8.4:720397-720416 | None:intergenic | 35.0% |
| ! | ACTAAAGCCATTAGTTTAGC+AGG | + | chr8.4:720227-720246 | MS.gene43433:intron | 35.0% |
| ! | CCTTATTTCCATTCAAGGTT+AGG | - | chr8.4:721546-721565 | None:intergenic | 35.0% |
| ! | CCTTCTTTTCTACAGAAATG+TGG | - | chr8.4:721310-721329 | None:intergenic | 35.0% |
| ! | CTTTCTAAAAGCAAGGGATT+GGG | + | chr8.4:718578-718597 | MS.gene43433:CDS | 35.0% |
| ! | GTCTAAAACCTTTACACAAG+TGG | - | chr8.4:719368-719387 | None:intergenic | 35.0% |
| ! | TAATAGCATTGGAGTCTGTA+TGG | + | chr8.4:719901-719920 | MS.gene43433:intron | 35.0% |
| ! | TAATTCCTGCAACTGATTCA+TGG | - | chr8.4:718381-718400 | None:intergenic | 35.0% |
| ! | TTTGATAGCCTAACCTTGAA+TGG | + | chr8.4:721535-721554 | MS.gene43433:CDS | 35.0% |
| !! | AACTATTCCAATTCTGGAAC+AGG | - | chr8.4:720507-720526 | None:intergenic | 35.0% |
| !! | AAGCCTTTGAAACTAGTTGT+TGG | + | chr8.4:720399-720418 | MS.gene43433:intron | 35.0% |
| !! | TCCTTCTAAGGCTTATCAAA+TGG | + | chr8.4:718602-718621 | MS.gene43433:CDS | 35.0% |
| !!! | CATATTTTCAGCTTGTTGGA+AGG | + | chr8.4:719799-719818 | MS.gene43433:CDS | 35.0% |
| !!! | GTAAAGGTTTTAGACATGAC+TGG | + | chr8.4:719373-719392 | MS.gene43433:CDS | 35.0% |
| !!! | TATTCCTTTTAGAACGCACA+CGG | + | chr8.4:721164-721183 | MS.gene43433:intron | 35.0% |
| ACCTGAAAAGTTGTCTGCAA+TGG | - | chr8.4:718422-718441 | None:intergenic | 40.0% | |
| AGATGTTGTTCCTTATGGCT+TGG | + | chr8.4:719854-719873 | MS.gene43433:intron | 40.0% | |
| AGTATTGCCTGTTCCAGAAT+TGG | + | chr8.4:720497-720516 | MS.gene43433:intron | 40.0% | |
| AGTTTAGCAGGATGGAACAT+AGG | + | chr8.4:720239-720258 | MS.gene43433:intron | 40.0% | |
| ATATCGCGATCGTGTAATCT+TGG | + | chr8.4:719219-719238 | MS.gene43433:CDS | 40.0% | |
| ATGAAATGATCCCTGCTTGA+TGG | - | chr8.4:721455-721474 | None:intergenic | 40.0% | |
| CCTGAAAAGTTGTCTGCAAT+GGG | - | chr8.4:718421-718440 | None:intergenic | 40.0% | |
| GCCTATTACCACTTGTGTAA+AGG | + | chr8.4:719357-719376 | MS.gene43433:CDS | 40.0% | |
| GTTCCATCCTGCTAAACTAA+TGG | - | chr8.4:720237-720256 | None:intergenic | 40.0% | |
| TAAAGTGCACCGAACGTTTA+CGG | - | chr8.4:720976-720995 | None:intergenic | 40.0% | |
| TCACAACTCATTGGATTGTC+CGG | + | chr8.4:718895-718914 | MS.gene43433:CDS | 40.0% | |
| TGAAATGATCCCTGCTTGAT+GGG | - | chr8.4:721454-721473 | None:intergenic | 40.0% | |
| TGGAGACATTCTCTAATCTG+AGG | - | chr8.4:720286-720305 | None:intergenic | 40.0% | |
| TTCTGTAGAAAAGAAGGCTC+TGG | + | chr8.4:721313-721332 | MS.gene43433:CDS | 40.0% | |
| TTCTTCTGACTCTCGTGATA+TGG | + | chr8.4:719302-719321 | MS.gene43433:intron | 40.0% | |
| ! | AAGCCATTAGTTTAGCAGGA+TGG | + | chr8.4:720231-720250 | MS.gene43433:intron | 40.0% |
| ! | AGAAAAGTGCAGGTCTTATC+AGG | + | chr8.4:719993-720012 | MS.gene43433:CDS | 40.0% |
| ! | AGTTGTAAAGCTGTTGGTGA+AGG | - | chr8.4:721393-721412 | None:intergenic | 40.0% |
| ! | ATTACCGTGTGCGTTCTAAA+AGG | - | chr8.4:721171-721190 | None:intergenic | 40.0% |
| ! | GACTCGCTTTCTAAAAGCAA+GGG | + | chr8.4:718572-718591 | MS.gene43433:CDS | 40.0% |
| ! | GCACTACTGTTAATTGGTTC+GGG | - | chr8.4:719541-719560 | None:intergenic | 40.0% |
| ! | GCTTTCTAAAAGCAAGGGAT+TGG | + | chr8.4:718577-718596 | MS.gene43433:CDS | 40.0% |
| ! | GTTGTAAAGCTGTTGGTGAA+GGG | - | chr8.4:721392-721411 | None:intergenic | 40.0% |
| ! | TGACTCGCTTTCTAAAAGCA+AGG | + | chr8.4:718571-718590 | MS.gene43433:CDS | 40.0% |
| ! | TTGTAAAGCTGTTGGTGAAG+GGG | - | chr8.4:721391-721410 | None:intergenic | 40.0% |
| !! | AACTAATGGCTTTAGTAGCG+TGG | - | chr8.4:720223-720242 | None:intergenic | 40.0% |
| !! | GAAAGTGCTCAATTAGTCTG+TGG | + | chr8.4:720546-720565 | MS.gene43433:intron | 40.0% |
| !!! | ATCAAGGTGTTGCTTTTCGT+TGG | + | chr8.4:719095-719114 | MS.gene43433:intron | 40.0% |
| AAGACAGGAAGACCCTGAAA+AGG | - | chr8.4:719026-719045 | None:intergenic | 45.0% | |
| AAGCGAGAACTGCTATTCCT+TGG | + | chr8.4:721360-721379 | MS.gene43433:CDS | 45.0% | |
| AAGGGATTGGGATCCTTCTA+AGG | + | chr8.4:718590-718609 | MS.gene43433:CDS | 45.0% | |
| ATTGTCCGGGTATACAAGAG+AGG | + | chr8.4:718909-718928 | MS.gene43433:CDS | 45.0% | |
| CAAGCAGGGATCATTTCATG+TGG | + | chr8.4:721456-721475 | MS.gene43433:CDS | 45.0% | |
| CACAACTCATTGGATTGTCC+GGG | + | chr8.4:718896-718915 | MS.gene43433:CDS | 45.0% | |
| CATGCAGAAACCAAGCCATA+AGG | - | chr8.4:719867-719886 | None:intergenic | 45.0% | |
| CCAACACCAATTGCAAAGAC+AGG | - | chr8.4:719041-719060 | None:intergenic | 45.0% | |
| CCTATTGGATCAAGTAGCAG+TGG | - | chr8.4:719444-719463 | None:intergenic | 45.0% | |
| CCTGTCTTTGCAATTGGTGT+TGG | + | chr8.4:719038-719057 | MS.gene43433:CDS | 45.0% | |
| CTCGGCCTTGAATCACATTA+AGG | + | chr8.4:719405-719424 | MS.gene43433:CDS | 45.0% | |
| CTTGTCCTCTCGTGTAAAAG+TGG | - | chr8.4:719972-719991 | None:intergenic | 45.0% | |
| CTTTGTGGTCCGTAAACGTT+CGG | + | chr8.4:720964-720983 | MS.gene43433:intron | 45.0% | |
| GAAAAGTTGTCTGCAATGGG+TGG | - | chr8.4:718418-718437 | None:intergenic | 45.0% | |
| GGATCATCCAGACATTCAGA+AGG | + | chr8.4:721334-721353 | MS.gene43433:CDS | 45.0% | |
| GGATTCCATGAATCAGTTGC+AGG | + | chr8.4:718373-718392 | MS.gene43433:CDS | 45.0% | |
| GGTCTTCCTGTCTTTGCAAT+TGG | + | chr8.4:719032-719051 | MS.gene43433:CDS | 45.0% | |
| TCATCCAGACATTCAGAAGG+TGG | + | chr8.4:721337-721356 | MS.gene43433:CDS | 45.0% | |
| TCTACACTTTCAACTGCGGT+TGG | - | chr8.4:720471-720490 | None:intergenic | 45.0% | |
| TGTGGAATTTCCAGAACCTC+CGG | + | chr8.4:721474-721493 | MS.gene43433:CDS | 45.0% | |
| ! | CAAAGGCTTTGTGGAGTGTA+AGG | - | chr8.4:720388-720407 | None:intergenic | 45.0% |
| ! | CCACTGCTACTTGATCCAAT+AGG | + | chr8.4:719441-719460 | MS.gene43433:intron | 45.0% |
| ! | CCCATTGCAGACAACTTTTC+AGG | + | chr8.4:718418-718437 | MS.gene43433:CDS | 45.0% |
| ! | GAACAATTGGACTAGAGCAC+AGG | + | chr8.4:721573-721592 | MS.gene43433:CDS | 45.0% |
| ! | GAGGACAAGAAGAAAAGTGC+AGG | + | chr8.4:719983-720002 | MS.gene43433:CDS | 45.0% |
| !!! | TGAAGCCACTTTTACACGAG+AGG | + | chr8.4:719964-719983 | MS.gene43433:CDS | 45.0% |
| !! | TCAAATATAAATAAATATAT+TGG | + | chr8.4:718466-718485 | MS.gene43433:intron | 5.0% |
| AACCTCCGGATGTGGATACA+AGG | + | chr8.4:721488-721507 | MS.gene43433:CDS | 50.0% | |
| ACCTCCGGATGTGGATACAA+GGG | + | chr8.4:721489-721508 | MS.gene43433:CDS | 50.0% | |
| ATGGAACATAGGTGCCTGCT+TGG | + | chr8.4:720250-720269 | MS.gene43433:intron | 50.0% | |
| ATTTCCAGAACCTCCGGATG+TGG | + | chr8.4:721480-721499 | MS.gene43433:CDS | 50.0% | |
| CCATGCTTCTTCGATGCAGA+AGG | - | chr8.4:719334-719353 | None:intergenic | 50.0% | |
| CCTTCTGCATCGAAGAAGCA+TGG | + | chr8.4:719331-719350 | MS.gene43433:CDS | 50.0% | |
| CGAACGTTTACGGACCACAA+AGG | - | chr8.4:720966-720985 | None:intergenic | 50.0% | |
| GAGCACCTCTCTTGTATACC+CGG | - | chr8.4:718917-718936 | None:intergenic | 50.0% | |
| GAGGCAAACAAATGCCAAGC+AGG | - | chr8.4:720267-720286 | None:intergenic | 50.0% | |
| GCAACCCTTGTATCCACATC+CGG | - | chr8.4:721496-721515 | None:intergenic | 50.0% | |
| GCTTCCACCTTCTGAATGTC+TGG | - | chr8.4:721344-721363 | None:intergenic | 50.0% | |
| TTGGTGAAGGGGATGATCCA+AGG | - | chr8.4:721380-721399 | None:intergenic | 50.0% | |
| ! | AGGCTTTGTGGAGTGTAAGG+AGG | - | chr8.4:720385-720404 | None:intergenic | 50.0% |
| ! | CTGCATCGAAGAAGCATGGA+CGG | + | chr8.4:719335-719354 | MS.gene43433:CDS | 50.0% |
| ! | GGCTTTGTGGAGTGTAAGGA+GGG | - | chr8.4:720384-720403 | None:intergenic | 50.0% |
| ! | GTGCAGGTCTTATCAGGATG+CGG | + | chr8.4:719999-720018 | MS.gene43433:CDS | 50.0% |
| ! | TGATGGGTGCAACAGCTTCA+CGG | - | chr8.4:721438-721457 | None:intergenic | 50.0% |
| !!! | GGTGTTGCTTTTCGTTGGAG+CGG | + | chr8.4:719100-719119 | MS.gene43433:intron | 50.0% |
| ACCCTTGTATCCACATCCGG+AGG | - | chr8.4:721493-721512 | None:intergenic | 55.0% | |
| AGCTGTTGCACCCATCAAGC+AGG | + | chr8.4:721441-721460 | MS.gene43433:CDS | 55.0% | |
| CTTATCAGGATGCGGACGAG+AGG | + | chr8.4:720007-720026 | MS.gene43433:CDS | 55.0% | |
| GATTCAAGGCCGAGAGCTTC+AGG | - | chr8.4:719399-719418 | None:intergenic | 55.0% | |
| GCTGTTGCACCCATCAAGCA+GGG | + | chr8.4:721442-721461 | MS.gene43433:CDS | 55.0% | |
| GTATCCACATCCGGAGGTTC+TGG | - | chr8.4:721487-721506 | None:intergenic | 55.0% | |
| TGCAACAGCTTCACGGAGTC+TGG | - | chr8.4:721431-721450 | None:intergenic | 55.0% | |
| !! | CATGACTGGCCTGAAGCTCT+CGG | + | chr8.4:719387-719406 | MS.gene43433:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 718371 | 721600 | 718371 | ID=MS.gene43433 |
| chr8.4 | mRNA | 718371 | 721600 | 718371 | ID=MS.gene43433.t1;Parent=MS.gene43433 |
| chr8.4 | exon | 718371 | 718439 | 718371 | ID=MS.gene43433.t1.exon1;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 718371 | 718439 | 718371 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 718540 | 718623 | 718540 | ID=MS.gene43433.t1.exon2;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 718540 | 718623 | 718540 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 718713 | 718766 | 718713 | ID=MS.gene43433.t1.exon3;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 718713 | 718766 | 718713 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 718853 | 718930 | 718853 | ID=MS.gene43433.t1.exon4;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 718853 | 718930 | 718853 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 719032 | 719085 | 719032 | ID=MS.gene43433.t1.exon5;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 719032 | 719085 | 719032 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 719181 | 719240 | 719181 | ID=MS.gene43433.t1.exon6;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 719181 | 719240 | 719181 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 719331 | 719426 | 719331 | ID=MS.gene43433.t1.exon7;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 719331 | 719426 | 719331 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 719719 | 719820 | 719719 | ID=MS.gene43433.t1.exon8;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 719719 | 719820 | 719719 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 719960 | 720040 | 719960 | ID=MS.gene43433.t1.exon9;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 719960 | 720040 | 719960 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| chr8.4 | exon | 721286 | 721600 | 721286 | ID=MS.gene43433.t1.exon10;Parent=MS.gene43433.t1 |
| chr8.4 | CDS | 721286 | 721600 | 721286 | ID=cds.MS.gene43433.t1;Parent=MS.gene43433.t1 |
| Gene Sequence |
| Protein sequence |
