Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44304.t1 | XP_024640520.1 | 98.3 | 115 | 1 | 1 | 1 | 114 | 1 | 115 | 2.20E-56 | 228 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44304.t1 | Q29Q81 | 50.0 | 110 | 44 | 6 | 5 | 106 | 4 | 110 | 4.6e-15 | 82.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44304.t1 | G7JYY6 | 98.3 | 115 | 1 | 1 | 1 | 114 | 1 | 115 | 1.6e-56 | 228.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene44304 | 0.816828 | 4.52E-52 | -1.69E-46 |
| MS.gene049808 | MS.gene44304 | 0.800447 | 1.44E-48 | -1.69E-46 |
| MS.gene050689 | MS.gene44304 | 0.806411 | 8.33E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene44304 | 0.804069 | 2.58E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene44304 | 0.819129 | 1.37E-52 | -1.69E-46 |
| MS.gene052514 | MS.gene44304 | 0.815882 | 7.36E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene44304 | 0.828865 | 7.09E-55 | -1.69E-46 |
| MS.gene052611 | MS.gene44304 | 0.8217 | 3.51E-53 | -1.69E-46 |
| MS.gene052612 | MS.gene44304 | 0.806651 | 7.42E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene44304 | 0.84107 | 5.94E-58 | -1.69E-46 |
| MS.gene054584 | MS.gene44304 | 0.841828 | 3.75E-58 | -1.69E-46 |
| MS.gene055465 | MS.gene44304 | 0.804906 | 1.73E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene44304 | 0.803323 | 3.68E-49 | -1.69E-46 |
| MS.gene055580 | MS.gene44304 | -0.817178 | 3.77E-52 | -1.69E-46 |
| MS.gene055890 | MS.gene44304 | 0.827149 | 1.83E-54 | -1.69E-46 |
| MS.gene056507 | MS.gene44304 | 0.803622 | 3.19E-49 | -1.69E-46 |
| MS.gene056545 | MS.gene44304 | 0.804308 | 2.30E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene44304 | 0.819498 | 1.13E-52 | -1.69E-46 |
| MS.gene057132 | MS.gene44304 | 0.816324 | 5.86E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene44304 | 0.811615 | 6.41E-51 | -1.69E-46 |
| MS.gene05894 | MS.gene44304 | 0.819915 | 9.04E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene44304 | 0.801117 | 1.05E-48 | -1.69E-46 |
| MS.gene060355 | MS.gene44304 | 0.807738 | 4.36E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44304.t1 | MTR_5g033220 | 98.261 | 115 | 1 | 1 | 1 | 114 | 1 | 115 | 2.27e-78 | 226 |
| MS.gene44304.t1 | MTR_8g069510 | 55.085 | 118 | 43 | 5 | 1 | 111 | 1 | 115 | 1.11e-28 | 100 |
| MS.gene44304.t1 | MTR_6g005250 | 44.444 | 108 | 57 | 2 | 5 | 112 | 7 | 111 | 2.88e-19 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44304.t1 | AT1G10690 | 40.566 | 106 | 50 | 3 | 7 | 106 | 8 | 106 | 1.82e-18 | 74.7 |
| MS.gene44304.t1 | AT1G60783 | 42.857 | 91 | 47 | 2 | 15 | 105 | 17 | 102 | 1.75e-17 | 72.0 |
| MS.gene44304.t1 | AT5G40460 | 47.826 | 115 | 48 | 7 | 1 | 107 | 1 | 111 | 9.19e-17 | 70.5 |
Find 26 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCTTGATCCTTCCCCTCT+TGG | 0.276061 | 5.4:+23318346 | None:intergenic |
| AACGAAAAGCTTCTTTGAAA+TGG | 0.305275 | 5.4:-23318288 | MS.gene44304:CDS |
| TCCAAAGGGATTGTATGTAT+TGG | 0.387019 | 5.4:+23318418 | None:intergenic |
| GCCAATACATACAATCCCTT+TGG | 0.438867 | 5.4:-23318419 | MS.gene44304:CDS |
| TGTTCATACGCCATGTTGAA+AGG | 0.478075 | 5.4:-23318213 | MS.gene44304:CDS |
| TGTATTGGCTTCAAATTCAA+TGG | 0.496731 | 5.4:+23318433 | None:intergenic |
| GGTGGACATATCAAAGTTGT+TGG | 0.496765 | 5.4:+23318322 | None:intergenic |
| CACCAAGAGGGGAAGGATCA+AGG | 0.500618 | 5.4:-23318348 | MS.gene44304:CDS |
| TTTCGTTTCCTCGGAGCTGG+TGG | 0.517258 | 5.4:+23318304 | None:intergenic |
| GTTCATACGCCATGTTGAAA+GGG | 0.550603 | 5.4:-23318212 | MS.gene44304:CDS |
| GACAATTCACAAGTGGAAGG+AGG | 0.590987 | 5.4:-23318505 | MS.gene44304:CDS |
| ACAATTCACAAGTGGAAGGA+GGG | 0.599492 | 5.4:-23318504 | MS.gene44304:CDS |
| TTTGGAGAAAAGTAAACAAG+AGG | 0.601702 | 5.4:-23318401 | MS.gene44304:CDS |
| TAGCTATAGCCCTTTCAACA+TGG | 0.602660 | 5.4:+23318203 | None:intergenic |
| ATGAACACAGTTTCTAACTC+AGG | 0.610457 | 5.4:+23318229 | None:intergenic |
| TCTGACAATTCACAAGTGGA+AGG | 0.624119 | 5.4:-23318508 | MS.gene44304:CDS |
| ATTATCTGACAATTCACAAG+TGG | 0.624603 | 5.4:-23318512 | MS.gene44304:CDS |
| TCAACAACACCAAGAGGGGA+AGG | 0.634273 | 5.4:-23318355 | MS.gene44304:CDS |
| AACACAGTTTCTAACTCAGG+TGG | 0.639915 | 5.4:+23318232 | None:intergenic |
| AGTGTTCAACAACACCAAGA+GGG | 0.640283 | 5.4:-23318360 | MS.gene44304:CDS |
| TAAACAAGAGGAAAGTGACA+TGG | 0.640838 | 5.4:-23318389 | MS.gene44304:CDS |
| GAGTGTTCAACAACACCAAG+AGG | 0.659812 | 5.4:-23318361 | MS.gene44304:CDS |
| GTGTTCAACAACACCAAGAG+GGG | 0.671532 | 5.4:-23318359 | MS.gene44304:CDS |
| TGATATGTCCACCAGCTCCG+AGG | 0.688116 | 5.4:-23318312 | MS.gene44304:CDS |
| TCTTTGAAATGGAATTATCG+CGG | 0.689964 | 5.4:-23318277 | MS.gene44304:CDS |
| ACAAGAGGAAAGTGACATGG+AGG | 0.701741 | 5.4:-23318386 | MS.gene44304:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAGAGAAAAATAGAAAA+TGG | - | chr5.4:23318244-23318263 | MS.gene44304:CDS | 20.0% |
| !! | TTCAGAGAAAAATAGAAAAT+GGG | - | chr5.4:23318245-23318264 | MS.gene44304:CDS | 20.0% |
| ATTATCTGACAATTCACAAG+TGG | - | chr5.4:23318206-23318225 | MS.gene44304:CDS | 30.0% | |
| TTTGGAGAAAAGTAAACAAG+AGG | - | chr5.4:23318317-23318336 | MS.gene44304:CDS | 30.0% | |
| ! | AACGAAAAGCTTCTTTGAAA+TGG | - | chr5.4:23318430-23318449 | MS.gene44304:CDS | 30.0% |
| !! | CTTGTTTACTTTTCTCCAAA+GGG | + | chr5.4:23318317-23318336 | None:intergenic | 30.0% |
| !! | TCTTGTTTACTTTTCTCCAA+AGG | + | chr5.4:23318318-23318337 | None:intergenic | 30.0% |
| !! | TCTTTGAAATGGAATTATCG+CGG | - | chr5.4:23318441-23318460 | MS.gene44304:CDS | 30.0% |
| !! | TGTATTGGCTTCAAATTCAA+TGG | + | chr5.4:23318288-23318307 | None:intergenic | 30.0% |
| ATGAACACAGTTTCTAACTC+AGG | + | chr5.4:23318492-23318511 | None:intergenic | 35.0% | |
| TAAACAAGAGGAAAGTGACA+TGG | - | chr5.4:23318329-23318348 | MS.gene44304:CDS | 35.0% | |
| TCCAAAGGGATTGTATGTAT+TGG | + | chr5.4:23318303-23318322 | None:intergenic | 35.0% | |
| ! | AAAGAAGCTTTTCGTTTCCT+CGG | + | chr5.4:23318426-23318445 | None:intergenic | 35.0% |
| AACACAGTTTCTAACTCAGG+TGG | + | chr5.4:23318489-23318508 | None:intergenic | 40.0% | |
| ACAATTCACAAGTGGAAGGA+GGG | - | chr5.4:23318214-23318233 | MS.gene44304:CDS | 40.0% | |
| AGTGTTCAACAACACCAAGA+GGG | - | chr5.4:23318358-23318377 | MS.gene44304:CDS | 40.0% | |
| GCCAATACATACAATCCCTT+TGG | - | chr5.4:23318299-23318318 | MS.gene44304:CDS | 40.0% | |
| GGTGGACATATCAAAGTTGT+TGG | + | chr5.4:23318399-23318418 | None:intergenic | 40.0% | |
| GTTCATACGCCATGTTGAAA+GGG | - | chr5.4:23318506-23318525 | MS.gene44304:CDS | 40.0% | |
| TAGCTATAGCCCTTTCAACA+TGG | + | chr5.4:23318518-23318537 | None:intergenic | 40.0% | |
| TCTGACAATTCACAAGTGGA+AGG | - | chr5.4:23318210-23318229 | MS.gene44304:CDS | 40.0% | |
| TGTTCATACGCCATGTTGAA+AGG | - | chr5.4:23318505-23318524 | MS.gene44304:CDS | 40.0% | |
| ACAAGAGGAAAGTGACATGG+AGG | - | chr5.4:23318332-23318351 | MS.gene44304:CDS | 45.0% | |
| GACAATTCACAAGTGGAAGG+AGG | - | chr5.4:23318213-23318232 | MS.gene44304:CDS | 45.0% | |
| GAGTGTTCAACAACACCAAG+AGG | - | chr5.4:23318357-23318376 | MS.gene44304:CDS | 45.0% | |
| GTGTTCAACAACACCAAGAG+GGG | - | chr5.4:23318359-23318378 | MS.gene44304:CDS | 45.0% | |
| ATCCTTGATCCTTCCCCTCT+TGG | + | chr5.4:23318375-23318394 | None:intergenic | 50.0% | |
| TCAACAACACCAAGAGGGGA+AGG | - | chr5.4:23318363-23318382 | MS.gene44304:CDS | 50.0% | |
| CACCAAGAGGGGAAGGATCA+AGG | - | chr5.4:23318370-23318389 | MS.gene44304:CDS | 55.0% | |
| TGATATGTCCACCAGCTCCG+AGG | - | chr5.4:23318406-23318425 | MS.gene44304:CDS | 55.0% | |
| TTTCGTTTCCTCGGAGCTGG+TGG | + | chr5.4:23318417-23318436 | None:intergenic | 55.0% | |
| ! | GCTTTTCGTTTCCTCGGAGC+TGG | + | chr5.4:23318420-23318439 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 23318198 | 23318542 | 23318198 | ID=MS.gene44304 |
| chr5.4 | mRNA | 23318198 | 23318542 | 23318198 | ID=MS.gene44304.t1;Parent=MS.gene44304 |
| chr5.4 | exon | 23318198 | 23318542 | 23318198 | ID=MS.gene44304.t1.exon1;Parent=MS.gene44304.t1 |
| chr5.4 | CDS | 23318198 | 23318542 | 23318198 | ID=cds.MS.gene44304.t1;Parent=MS.gene44304.t1 |
| Gene Sequence |
| Protein sequence |
