AlfalfaGEDB | MS.gene45038

M. sativa cultivar XinJiangDaYe / MS.gene45038

KEGG: -
GO: -
PFAM: PF00045.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45038.t1	XP_003590505.1	93.7	222	14	0	1	222	1	222	4.60E-119	437.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45038.t1	P08688	44.6	222	115	6	6	220	7	227	2.9e-45	183.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45038.t1	G7IBL7	93.7	222	14	0	1	222	1	222	3.3e-119	437.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050720	MS.gene45038	0.811387	7.18E-51	-1.69E-46
MS.gene050725	MS.gene45038	0.80302	4.25E-49	-1.69E-46
MS.gene051439	MS.gene45038	0.803691	3.09E-49	-1.69E-46
MS.gene051973	MS.gene45038	0.801102	1.06E-48	-1.69E-46
MS.gene051996	MS.gene45038	0.800888	1.17E-48	-1.69E-46
MS.gene052606	MS.gene45038	0.800291	1.54E-48	-1.69E-46
MS.gene054742	MS.gene45038	0.821528	3.85E-53	-1.69E-46
MS.gene056633	MS.gene45038	-0.819033	1.44E-52	-1.69E-46
MS.gene056678	MS.gene45038	0.809302	2.02E-50	-1.69E-46
MS.gene059892	MS.gene45038	0.811629	6.36E-51	-1.69E-46
MS.gene060087	MS.gene45038	0.85518	7.50E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45038.t1	MTR_1g066530	93.694	222	14	0	1	222	1	222	7.68e-157	433
MS.gene45038.t1	MTR_6g073540	50.000	230	106	2	1	221	1	230	1.39e-74	225
MS.gene45038.t1	MTR_6g072470	51.500	200	91	1	1	194	1	200	1.28e-67	207
MS.gene45038.t1	MTR_6g048620	48.649	222	107	5	6	220	5	226	6.78e-65	201
MS.gene45038.t1	MTR_6g045077	45.000	220	116	3	4	220	3	220	4.33e-63	196
MS.gene45038.t1	MTR_6g048910	45.455	220	113	4	6	220	5	222	2.58e-62	194
MS.gene45038.t1	MTR_0695s0030	44.545	220	117	4	6	220	65	284	8.83e-62	195
MS.gene45038.t1	MTR_8g038100	45.000	220	116	4	6	220	281	500	1.48e-59	196
MS.gene45038.t1	MTR_8g038070	44.545	220	117	4	6	220	219	438	2.16e-59	193
MS.gene45038.t1	MTR_0057s0100	41.364	220	124	5	6	220	7	226	9.02e-54	172
MS.gene45038.t1	MTR_0057s0170	41.364	220	124	5	6	220	7	226	9.92e-53	169
MS.gene45038.t1	MTR_7g103990	41.704	223	120	8	6	220	7	227	4.09e-50	163
MS.gene45038.t1	MTR_0216s0030	40.404	198	110	3	4	197	1	194	1.04e-47	155
MS.gene45038.t1	MTR_0807s0020	37.500	224	130	5	1	220	1	218	3.17e-46	152
MS.gene45038.t1	MTR_6g040290	40.359	223	125	6	6	220	7	229	1.59e-45	151
MS.gene45038.t1	MTR_6g048880	40.449	178	96	5	50	220	4	178	1.42e-39	134
MS.gene45038.t1	MTR_7g012000	39.904	208	95	5	6	209	7	188	3.38e-39	134
MS.gene45038.t1	MTR_6g048810	50.355	141	64	4	84	220	162	300	6.79e-37	131
MS.gene45038.t1	MTR_4g102470	40.556	180	84	5	6	182	7	166	2.38e-33	119
MS.gene45038.t1	MTR_6g049040	40.146	137	65	3	1	135	1	122	5.73e-25	95.9
MS.gene45038.t1	MTR_6g049040	35.043	117	65	2	72	187	16	122	3.13e-16	72.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 44 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGGTACGGTCTTTGAAAA+TGG	0.203555	1.2:+49994052	MS.gene45038:CDS
CTTGAACACGTAACATTCAT+AGG	0.229009	1.2:-49993615	None:intergenic
ATCCGGAGCGAAATCTATTT+TGG	0.242862	1.2:-49993819	None:intergenic
TTTAACAACACGATCCTTCT+TGG	0.279901	1.2:-49993675	None:intergenic
TAGATCTCTAGAAGTTTAAT+AGG	0.303436	1.2:-49994225	None:intergenic
ACTCATTACTCCATTCGTTC+TGG	0.346885	1.2:+49994016	MS.gene45038:CDS
AATGGTGTTTCATCAAGAAA+TGG	0.395080	1.2:-49993725	None:intergenic
TCCAGAGAAGATTATTGCTT+CGG	0.411108	1.2:-49993786	None:intergenic
AACATGGTAGTGATTGGAAC+AGG	0.421529	1.2:-49993866	None:intergenic
GTTACGTGTTCAAGAAGAAA+AGG	0.439700	1.2:+49993624	MS.gene45038:CDS
ACACGATCCTTCTTGGCTCC+TGG	0.452509	1.2:-49993668	None:intergenic
CAATGGAACATGGTAGTGAT+TGG	0.462327	1.2:-49993872	None:intergenic
GAATAAGAAAACATTATGTC+CGG	0.469058	1.2:-49993945	None:intergenic
TGATGAAACACCATTTGAAA+AGG	0.482259	1.2:+49993733	MS.gene45038:CDS
ACCGAAGCAATAATCTTCTC+TGG	0.483775	1.2:+49993785	MS.gene45038:CDS
TTTAATAGGCTCTGAAGAGC+AGG	0.498729	1.2:-49994211	None:intergenic
GTTCAAGAAGAAAAGGTTTG+CGG	0.504927	1.2:+49993631	MS.gene45038:CDS
AACAGGGCCATCGAGTAAAT+CGG	0.507747	1.2:-49993849	None:intergenic
TTGAACACGTAACATTCATA+GGG	0.512326	1.2:-49993614	None:intergenic
AGTAAATCGGTATCTGAATC+CGG	0.514277	1.2:-49993836	None:intergenic
GCGGTTGTGAATTATGCTCC+AGG	0.516853	1.2:+49993650	MS.gene45038:CDS
TGTCAATGGTAGAGTAAAGC+TGG	0.518786	1.2:+49994174	MS.gene45038:CDS
GACAATACAGTCGCCTTCGT+TGG	0.522082	1.2:-49994155	None:intergenic
GACGCTGCAATTAAGTGGAC+CGG	0.527798	1.2:+49993926	MS.gene45038:CDS
AAACCATCGGAAGTATGAAT+CGG	0.529141	1.2:-49993701	None:intergenic
TTCGGTGCCTTCTGTCTCAA+AGG	0.532520	1.2:-49993768	None:intergenic
GAATAGACGCTGCAATTAAG+TGG	0.535911	1.2:+49993921	MS.gene45038:CDS
AGGAAATGAGTATGTTCGTA+TGG	0.536361	1.2:+49993970	MS.gene45038:CDS
TACCATGTTCCATTGTCTCA+AGG	0.539896	1.2:+49993880	MS.gene45038:CDS
GAAGAGCAGGCCATTCATCA+TGG	0.540017	1.2:-49994198	None:intergenic
AAGCCGATTCATACTTCCGA+TGG	0.550807	1.2:+49993698	MS.gene45038:CDS
TAAAGCTGGTCCATGATGAA+TGG	0.552708	1.2:+49994188	MS.gene45038:CDS
AAAACGTTCCCTTGAGACAA+TGG	0.561335	1.2:-49993889	None:intergenic
TGCCAAAATAGATTTCGCTC+CGG	0.565519	1.2:+49993817	MS.gene45038:CDS
ATCAAGAAATGGAAAACCAT+CGG	0.570603	1.2:-49993714	None:intergenic
GATTGTGCCTTTGAGACAGA+AGG	0.572261	1.2:+49993761	MS.gene45038:CDS
ACCATGTTCCATTGTCTCAA+GGG	0.576700	1.2:+49993881	MS.gene45038:CDS
GAAATGCAGTATCAATGACT+TGG	0.591249	1.2:-49993580	None:intergenic
TTATGCTCCAGGAGCCAAGA+AGG	0.592184	1.2:+49993661	MS.gene45038:CDS
TCAGATACCGATTTACTCGA+TGG	0.596226	1.2:+49993842	MS.gene45038:CDS
GAAGGCGACTGTATTGTCAA+TGG	0.604836	1.2:+49994160	MS.gene45038:CDS
CGTATTGATATTTCCAACGA+AGG	0.614900	1.2:+49994142	MS.gene45038:CDS
TCCCTTGAGACAATGGAACA+TGG	0.652701	1.2:-49993882	None:intergenic
ACATGGTAGTGATTGGAACA+GGG	0.671114	1.2:-49993865	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CATAATGTTTTCTTATTCAA+AGG	+	chr1.2:49993950-49993969	MS.gene45038:CDS	20.0%
!	GAATAAGAAAACATTATGTC+CGG	-	chr1.2:49993948-49993967	None:intergenic	25.0%
	AATGGTGTTTCATCAAGAAA+TGG	-	chr1.2:49993728-49993747	None:intergenic	30.0%
	ATCAAGAAATGGAAAACCAT+CGG	-	chr1.2:49993717-49993736	None:intergenic	30.0%
	TGATGAAACACCATTTGAAA+AGG	+	chr1.2:49993733-49993752	MS.gene45038:CDS	30.0%
	TTGAACACGTAACATTCATA+GGG	-	chr1.2:49993617-49993636	None:intergenic	30.0%
!	AATCAAGCTTACGTTTTCAA+AGG	+	chr1.2:49994106-49994125	MS.gene45038:CDS	30.0%
!	CAATCTACTTCCTTTTCAAA+TGG	-	chr1.2:49993746-49993765	None:intergenic	30.0%
!!	AAGGGAACGTTTTTAGAAAA+TGG	+	chr1.2:49993899-49993918	MS.gene45038:CDS	30.0%
!!	TTTTAAAAGTTTGCTCGGTA+CGG	+	chr1.2:49994039-49994058	MS.gene45038:CDS	30.0%
!!!	ACTTTTAAAACCAGAACGAA+TGG	-	chr1.2:49994029-49994048	None:intergenic	30.0%
!!!	TCTGGTTTTAAAAGTTTGCT+CGG	+	chr1.2:49994034-49994053	MS.gene45038:CDS	30.0%
	AGGAAATGAGTATGTTCGTA+TGG	+	chr1.2:49993970-49993989	MS.gene45038:CDS	35.0%
	AGTAAATCGGTATCTGAATC+CGG	-	chr1.2:49993839-49993858	None:intergenic	35.0%
	CTTGAACACGTAACATTCAT+AGG	-	chr1.2:49993618-49993637	None:intergenic	35.0%
	GAAATGCAGTATCAATGACT+TGG	-	chr1.2:49993583-49993602	None:intergenic	35.0%
	GTTACGTGTTCAAGAAGAAA+AGG	+	chr1.2:49993624-49993643	MS.gene45038:CDS	35.0%
	GTTCAAGAAGAAAAGGTTTG+CGG	+	chr1.2:49993631-49993650	MS.gene45038:CDS	35.0%
	TCCAGAGAAGATTATTGCTT+CGG	-	chr1.2:49993789-49993808	None:intergenic	35.0%
	TTTAACAACACGATCCTTCT+TGG	-	chr1.2:49993678-49993697	None:intergenic	35.0%
!	AAACCATCGGAAGTATGAAT+CGG	-	chr1.2:49993704-49993723	None:intergenic	35.0%
!	CGTATTGATATTTCCAACGA+AGG	+	chr1.2:49994142-49994161	MS.gene45038:CDS	35.0%
	AAAACGTTCCCTTGAGACAA+TGG	-	chr1.2:49993892-49993911	None:intergenic	40.0%
	ACCATGTTCCATTGTCTCAA+GGG	+	chr1.2:49993881-49993900	MS.gene45038:CDS	40.0%
	ACCGAAGCAATAATCTTCTC+TGG	+	chr1.2:49993785-49993804	MS.gene45038:CDS	40.0%
	ACTCATTACTCCATTCGTTC+TGG	+	chr1.2:49994016-49994035	MS.gene45038:CDS	40.0%
	GAATAGACGCTGCAATTAAG+TGG	+	chr1.2:49993921-49993940	MS.gene45038:CDS	40.0%
	TAAAGCTGGTCCATGATGAA+TGG	+	chr1.2:49994188-49994207	MS.gene45038:CDS	40.0%
	TACCATGTTCCATTGTCTCA+AGG	+	chr1.2:49993880-49993899	MS.gene45038:CDS	40.0%
	TGCCAAAATAGATTTCGCTC+CGG	+	chr1.2:49993817-49993836	MS.gene45038:CDS	40.0%
	TGTCAATGGTAGAGTAAAGC+TGG	+	chr1.2:49994174-49994193	MS.gene45038:CDS	40.0%
!	AACATGGTAGTGATTGGAAC+AGG	-	chr1.2:49993869-49993888	None:intergenic	40.0%
!	ACATGGTAGTGATTGGAACA+GGG	-	chr1.2:49993868-49993887	None:intergenic	40.0%
!	ATCCGGAGCGAAATCTATTT+TGG	-	chr1.2:49993822-49993841	None:intergenic	40.0%
!	CAATGGAACATGGTAGTGAT+TGG	-	chr1.2:49993875-49993894	None:intergenic	40.0%
!!	TCAGATACCGATTTACTCGA+TGG	+	chr1.2:49993842-49993861	MS.gene45038:CDS	40.0%
!!	TTTAATAGGCTCTGAAGAGC+AGG	-	chr1.2:49994214-49994233	None:intergenic	40.0%
	AACAGGGCCATCGAGTAAAT+CGG	-	chr1.2:49993852-49993871	None:intergenic	45.0%
	GAAGGCGACTGTATTGTCAA+TGG	+	chr1.2:49994160-49994179	MS.gene45038:CDS	45.0%
	TCCCTTGAGACAATGGAACA+TGG	-	chr1.2:49993885-49993904	None:intergenic	45.0%
!	AAGCCGATTCATACTTCCGA+TGG	+	chr1.2:49993698-49993717	MS.gene45038:CDS	45.0%
!	CTCGGTACGGTCTTTGAAAA+TGG	+	chr1.2:49994052-49994071	MS.gene45038:CDS	45.0%
!!	GATTGTGCCTTTGAGACAGA+AGG	+	chr1.2:49993761-49993780	MS.gene45038:CDS	45.0%
	GAAGAGCAGGCCATTCATCA+TGG	-	chr1.2:49994201-49994220	None:intergenic	50.0%
	GACAATACAGTCGCCTTCGT+TGG	-	chr1.2:49994158-49994177	None:intergenic	50.0%
	GACGCTGCAATTAAGTGGAC+CGG	+	chr1.2:49993926-49993945	MS.gene45038:CDS	50.0%
	GCGGTTGTGAATTATGCTCC+AGG	+	chr1.2:49993650-49993669	MS.gene45038:CDS	50.0%
	TTATGCTCCAGGAGCCAAGA+AGG	+	chr1.2:49993661-49993680	MS.gene45038:CDS	50.0%
!!	TTCGGTGCCTTCTGTCTCAA+AGG	-	chr1.2:49993771-49993790	None:intergenic	50.0%
	ACACGATCCTTCTTGGCTCC+TGG	-	chr1.2:49993671-49993690	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	49993572	49994240	49993572	ID=MS.gene45038
chr1.2	mRNA	49993572	49994240	49993572	ID=MS.gene45038.t1;Parent=MS.gene45038
chr1.2	exon	49993572	49994240	49993572	ID=MS.gene45038.t1.exon1;Parent=MS.gene45038.t1
chr1.2	CDS	49993572	49994240	49993572	ID=cds.MS.gene45038.t1;Parent=MS.gene45038.t1

Gene Sequence

>MS.gene45038

ATGTCAATCCAAGTCATTGATACTGCATTTCGTTCATCTAAGCCCTATGAATGTTACGTGTTCAAGAAGAAAAGGTTTGCGGTTGTGAATTATGCTCCAGGAGCCAAGAAGGATCGTGTTGTTAAAAAGCCGATTCATACTTCCGATGGTTTTCCATTTCTTGATGAAACACCATTTGAAAAGGAAGTAGATTGTGCCTTTGAGACAGAAGGCACCGAAGCAATAATCTTCTCTGGAAAGCAATGTGCCAAAATAGATTTCGCTCCGGATTCAGATACCGATTTACTCGATGGCCCTGTTCCAATCACTACCATGTTCCATTGTCTCAAGGGAACGTTTTTAGAAAATGGAATAGACGCTGCAATTAAGTGGACCGGACATAATGTTTTCTTATTCAAAGGAAATGAGTATGTTCGTATGGACTATCACAAGAAGACTATTGTGACTCATTACTCCATTCGTTCTGGTTTTAAAAGTTTGCTCGGTACGGTCTTTGAAAATGGAATTGATGCAGCATTTGCTTCTCATGTTGCAAATCAAGCTTACGTTTTCAAAGGAGAATACTATGCACGTATTGATATTTCCAACGAAGGCGACTGTATTGTCAATGGTAGAGTAAAGCTGGTCCATGATGAATGGCCTGCTCTTCAGAGCCTATTAAACTTCTAG

Protein sequence

>MS.gene45038.t1

MSIQVIDTAFRSSKPYECYVFKKKRFAVVNYAPGAKKDRVVKKPIHTSDGFPFLDETPFEKEVDCAFETEGTEAIIFSGKQCAKIDFAPDSDTDLLDGPVPITTMFHCLKGTFLENGIDAAIKWTGHNVFLFKGNEYVRMDYHKKTIVTHYSIRSGFKSLLGTVFENGIDAAFASHVANQAYVFKGEYYARIDISNEGDCIVNGRVKLVHDEWPALQSLLNF