AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050725


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050725.t1 MTR_7g111010 96.957 230 7 0 1 230 1 230 4.05e-170 469
MS.gene050725.t1 MTR_7g110990 91.739 230 4 1 1 230 1 215 9.37e-156 432
MS.gene050725.t1 MTR_1g085460 87.500 216 27 0 15 230 18 233 1.58e-144 404
MS.gene050725.t1 MTR_1g026020 63.717 226 81 1 4 229 6 230 1.24e-107 310
MS.gene050725.t1 MTR_2g097030 58.333 240 92 4 1 235 4 240 1.23e-98 288
MS.gene050725.t1 MTR_5g075320 59.657 233 85 4 8 235 5 233 1.49e-98 287
MS.gene050725.t1 MTR_7g061720 58.750 240 90 5 1 234 1 237 1.57e-97 285
MS.gene050725.t1 MTR_5g079950 59.504 242 88 5 1 235 1 239 2.51e-97 285
MS.gene050725.t1 MTR_3g435430 66.667 204 64 3 27 230 25 224 2.03e-96 282
MS.gene050725.t1 MTR_3g094040 60.793 227 84 4 5 230 8 230 2.71e-96 282
MS.gene050725.t1 MTR_3g094060 65.686 204 66 3 27 230 31 230 3.48e-96 281
MS.gene050725.t1 MTR_5g041700 65.385 208 68 3 27 234 25 228 5.80e-96 281
MS.gene050725.t1 MTR_4g081950 65.385 208 68 3 27 234 61 264 1.90e-95 281
MS.gene050725.t1 MTR_7g011060 61.572 229 74 5 9 235 6 222 6.70e-94 275
MS.gene050725.t1 MTR_4g063975 58.667 225 85 4 1 220 2 223 1.93e-93 275
MS.gene050725.t1 MTR_3g466790 58.874 231 91 3 5 235 10 236 1.29e-91 270
MS.gene050725.t1 MTR_3g466760 58.442 231 92 3 5 235 16 242 7.05e-90 266
MS.gene050725.t1 MTR_6g012520 64.593 209 62 4 27 235 28 224 3.93e-84 251
MS.gene050725.t1 MTR_5g011210 49.573 234 109 4 4 232 10 239 2.90e-80 241
MS.gene050725.t1 MTR_4g102450 50.862 232 107 3 5 232 10 238 3.98e-80 241
MS.gene050725.t1 MTR_7g013120 49.776 223 104 4 9 230 14 229 3.77e-78 236
MS.gene050725.t1 MTR_4g124340 52.195 205 93 3 27 230 61 261 9.86e-78 236
MS.gene050725.t1 MTR_7g013150 50.000 228 105 5 4 230 10 229 6.66e-75 228
MS.gene050725.t1 MTR_7g013140 51.570 223 102 4 9 230 10 227 1.36e-74 226
MS.gene050725.t1 MTR_8g464350 48.908 229 104 5 10 230 7 230 2.36e-72 221
MS.gene050725.t1 MTR_8g058990 50.732 205 95 4 27 230 30 229 5.03e-69 213
MS.gene050725.t1 MTR_0040s0140 45.339 236 125 3 1 235 6 238 8.67e-67 207
MS.gene050725.t1 MTR_2g090255 44.492 236 125 4 1 235 4 234 2.90e-64 200
MS.gene050725.t1 MTR_5g076950 46.919 211 105 3 27 235 41 246 9.57e-63 197
MS.gene050725.t1 MTR_3g064510 45.581 215 110 5 27 235 53 266 7.98e-59 187
MS.gene050725.t1 MTR_6g012500 57.831 83 34 1 153 235 181 262 3.96e-25 100
MS.gene050725.t1 MTR_2g090270 30.233 215 124 8 27 230 35 234 8.37e-24 96.3
MS.gene050725.t1 MTR_0008s0120 31.579 228 95 7 4 230 8 175 4.92e-23 92.8
MS.gene050725.t1 MTR_2g090265 29.508 244 142 10 2 230 4 232 1.13e-22 93.2
MS.gene050725.t1 MTR_0122s0020 32.474 194 105 9 27 209 41 219 8.87e-20 85.5
MS.gene050725.t1 MTR_4g028640 30.732 205 109 9 17 209 32 215 9.08e-19 82.8
MS.gene050725.t1 MTR_4g099370 27.426 237 143 10 5 233 11 226 2.62e-18 81.3
MS.gene050725.t1 MTR_0809s0010 30.457 197 105 9 27 210 47 224 2.60e-17 79.0
MS.gene050725.t1 MTR_0118s0070 31.250 192 104 8 32 210 53 229 2.97e-17 79.0
MS.gene050725.t1 MTR_5g013440 26.872 227 143 10 5 226 12 220 1.80e-16 76.3
MS.gene050725.t1 MTR_4g075790 31.280 211 121 10 28 231 24 217 2.78e-15 73.2
MS.gene050725.t1 MTR_0122s0020 31.818 176 94 9 27 191 41 201 4.00e-14 69.3
MS.gene050725.t1 MTR_1g085365 84.848 33 5 0 198 230 4 36 3.37e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050725.t1 AT4G01630 76.087 230 54 1 1 229 1 230 1.70e-132 374
MS.gene050725.t1 AT5G05290 59.052 232 86 3 10 235 9 237 9.62e-100 291
MS.gene050725.t1 AT1G20190 68.342 199 62 1 31 229 30 227 1.97e-99 290
MS.gene050725.t1 AT2G40610 60.538 223 85 2 8 230 11 230 9.37e-98 285
MS.gene050725.t1 AT1G69530 66.667 204 64 3 27 230 27 226 3.53e-97 285
MS.gene050725.t1 AT1G69530 66.667 204 64 3 27 230 27 226 4.56e-97 284
MS.gene050725.t1 AT1G69530 66.667 204 64 3 27 230 27 226 4.56e-97 284
MS.gene050725.t1 AT2G28950 60.262 229 81 5 1 224 3 226 2.73e-95 279
MS.gene050725.t1 AT2G03090 63.462 208 72 3 27 234 30 233 1.04e-94 278
MS.gene050725.t1 AT5G56320 57.826 230 93 3 5 234 7 232 3.40e-94 276
MS.gene050725.t1 AT5G56320 57.826 230 93 3 5 234 7 232 3.40e-94 276
MS.gene050725.t1 AT3G55500 57.384 237 93 4 4 235 7 240 3.85e-94 276
MS.gene050725.t1 AT5G02260 56.540 237 95 4 3 234 4 237 4.15e-94 276
MS.gene050725.t1 AT2G39700 63.033 211 74 3 26 235 30 237 7.24e-94 276
MS.gene050725.t1 AT5G56320 57.826 230 93 3 5 234 7 232 2.21e-93 276
MS.gene050725.t1 AT2G37640 63.333 210 73 3 27 235 36 242 2.63e-93 275
MS.gene050725.t1 AT3G29030 53.279 244 90 4 5 235 3 235 3.25e-90 266
MS.gene050725.t1 AT1G69530 63.547 203 68 4 27 229 27 223 2.24e-89 264
MS.gene050725.t1 AT1G69530 65.641 195 61 4 27 221 27 215 5.53e-89 262
MS.gene050725.t1 AT1G26770 59.649 228 88 3 8 235 7 230 2.56e-88 261
MS.gene050725.t1 AT1G26770 59.649 228 88 3 8 235 17 240 3.68e-88 261
MS.gene050725.t1 AT3G15370 52.381 231 102 3 1 230 3 226 2.36e-82 246
MS.gene050725.t1 AT2G37640 60.104 193 73 3 44 235 28 217 6.73e-80 239
MS.gene050725.t1 AT1G20190 65.476 168 57 1 62 229 2 168 9.81e-79 235
MS.gene050725.t1 AT1G12560 56.061 198 83 3 27 224 37 230 3.01e-77 234
MS.gene050725.t1 AT3G15370 46.360 261 102 4 1 230 3 256 1.40e-75 230
MS.gene050725.t1 AT1G62980 49.333 225 109 4 1 224 5 225 2.28e-74 226
MS.gene050725.t1 AT5G39310 51.675 209 95 4 21 229 84 286 5.75e-73 224
MS.gene050725.t1 AT5G39270 53.202 203 89 4 27 229 58 254 2.00e-72 222
MS.gene050725.t1 AT5G39290 52.709 203 90 4 27 229 58 254 6.78e-71 218
MS.gene050725.t1 AT5G39300 51.724 203 92 4 27 229 55 251 1.14e-70 218
MS.gene050725.t1 AT5G39280 51.232 203 93 4 27 229 54 250 3.65e-70 216
MS.gene050725.t1 AT4G38210 44.538 238 123 5 1 235 5 236 1.20e-63 199
MS.gene050725.t1 AT3G03220 45.024 211 109 3 27 235 40 245 4.87e-61 192
MS.gene050725.t1 AT5G39260 47.087 206 97 5 25 229 43 237 6.33e-55 177
MS.gene050725.t1 AT4G28250 31.839 223 123 8 1 209 7 214 7.09e-26 102
MS.gene050725.t1 AT2G20750 33.184 223 118 9 6 209 11 221 3.06e-25 100
MS.gene050725.t1 AT4G28250 30.631 222 121 8 1 209 7 208 1.50e-22 92.8
MS.gene050725.t1 AT1G65680 32.110 218 119 11 27 230 47 249 5.78e-20 86.3
MS.gene050725.t1 AT2G20750 32.683 205 107 9 6 191 11 203 1.24e-19 84.3
MS.gene050725.t1 AT2G45110 33.180 217 111 10 30 231 36 233 7.24e-18 80.5
MS.gene050725.t1 AT4G28250 29.665 209 114 8 1 196 7 195 1.56e-17 78.6
MS.gene050725.t1 AT4G28250 29.902 204 110 8 1 191 7 190 9.04e-17 76.3
MS.gene050725.t1 AT4G17030 25.118 211 143 6 1 207 1 200 1.05e-13 68.9
MS.gene050725.t1 AT3G60570 28.191 188 106 7 27 201 34 205 2.23e-12 65.1

Find 0 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAATTAAAGTAACAC+CGG - 50571:6161-6180 MS.gene050725:CDS 15.0%
!! AACATAAGATTTACCATAAA+TGG - 50571:6389-6408 MS.gene050725:intron 20.0%
!! ACATAAGATTTACCATAAAT+GGG - 50571:6390-6409 MS.gene050725:intron 20.0%
!! TTAGAATTCAACTCAAAAAT+GGG - 50571:6576-6595 MS.gene050725:CDS 20.0%
!!! ATTTCTAAAGTTTGGATAAA+AGG - 50571:6464-6483 MS.gene050725:intron 20.0%
!!! CTTTATTTTGATTAACTTGT+AGG - 50571:5696-5715 MS.gene050725:CDS 20.0%
!!! TTTAGAATTCAACTCAAAAA+TGG - 50571:6575-6594 MS.gene050725:CDS 20.0%
!!! TTTCTAAAGTTTGGATAAAA+GGG - 50571:6465-6484 MS.gene050725:intron 20.0%
! AAGGGTCAAAAAAGAATAAA+TGG - 50571:6483-6502 MS.gene050725:intron 25.0%
! AGGGTCAAAAAAGAATAAAT+GGG - 50571:6484-6503 MS.gene050725:intron 25.0%
! GTATTTATATATGCAGAGTT+GGG - 50571:6351-6370 MS.gene050725:CDS 25.0%
! TGTATTTATATATGCAGAGT+TGG - 50571:6350-6369 MS.gene050725:CDS 25.0%
!!! TATTTTGATTAACTTGTAGG+TGG - 50571:5699-5718 MS.gene050725:CDS 25.0%
AAAATAGTCTCTCCCATTTA+TGG + 50571:6405-6424 None:intergenic 30.0%
AATTCAACTCAAAAATGGGA+AGG - 50571:6580-6599 MS.gene050725:CDS 30.0%
ACTAAAAATACTTGTTCGCT+AGG + 50571:6270-6289 None:intergenic 30.0%
AGTTTGACAATGAAACATCA+AGG + 50571:6221-6240 None:intergenic 30.0%
ATTAATTAAAGTAACACCGG+TGG - 50571:6164-6183 MS.gene050725:CDS 30.0%
GTTTGACAATGAAACATCAA+GGG + 50571:6220-6239 None:intergenic 30.0%
!!! TAGCGAACAAGTATTTTTAG+TGG - 50571:6269-6288 MS.gene050725:CDS 30.0%
AATCTGTACACAGATGGATA+TGG - 50571:5738-5757 MS.gene050725:intron 35.0%
ACATACCTCCTGTACAAAAT+GGG + 50571:6026-6045 None:intergenic 35.0%
ACATATCAAAGTGAGGTCTA+GGG + 50571:5956-5975 None:intergenic 35.0%
AGTACTGCTCTCTTTAATGA+TGG - 50571:5780-5799 MS.gene050725:intron 35.0%
ATTTGTGGCAGTAATAGTGA+TGG + 50571:5880-5899 None:intergenic 35.0%
CAAAGCCTAAGCTATCTTAA+TGG - 50571:6539-6558 MS.gene050725:CDS 35.0%
GTACTGCTCTCTTTAATGAT+GGG - 50571:5781-5800 MS.gene050725:intron 35.0%
GTACTTAGCTATAGTCTGAA+AGG + 50571:5988-6007 None:intergenic 35.0%
TATGGAAATCTGTACACAGA+TGG - 50571:5732-5751 MS.gene050725:intron 35.0%
TCAGAGTAGAATCTGTTGTT+TGG - 50571:5533-5552 MS.gene050725:CDS 35.0%
TCTGACCATTAAGATAGCTT+AGG + 50571:6547-6566 None:intergenic 35.0%
! ATCAGATAGTTTGTGATGCA+AGG - 50571:5823-5842 MS.gene050725:intron 35.0%
! GAATTGTTCCCATTTTGTAC+AGG - 50571:6015-6034 MS.gene050725:intron 35.0%
! GTTTGGTGGACAAAAATTTG+TGG + 50571:5895-5914 None:intergenic 35.0%
! TTGGTGCTTATAAGCAATGT+AGG - 50571:6428-6447 MS.gene050725:intron 35.0%
! TTTAATGATGGGAAGTCATG+TGG - 50571:5792-5811 MS.gene050725:intron 35.0%
!! GTGGAGAGATTTCTAAAGTT+TGG - 50571:6456-6475 MS.gene050725:intron 35.0%
ACCAACCACCATTGTCATTA+GGG + 50571:5926-5945 None:intergenic 40.0%
ACTATAGCTAAGTACAGAGC+TGG - 50571:5993-6012 MS.gene050725:intron 40.0%
ATATCAAAGTGAGGTCTAGG+GGG + 50571:5954-5973 None:intergenic 40.0%
CAAGCTCATGCAACTTTCTA+TGG - 50571:5559-5578 MS.gene050725:CDS 40.0%
CAATTTACCCATTGTTCCTG+AGG + 50571:5596-5615 None:intergenic 40.0%
CATATCAAAGTGAGGTCTAG+GGG + 50571:5955-5974 None:intergenic 40.0%
CTTATAAGCAATGTAGGTGG+AGG - 50571:6434-6453 MS.gene050725:intron 40.0%
GAACCGATGTCAATGAATTG+GGG - 50571:6506-6525 MS.gene050725:CDS 40.0%
GACAATGAAACATCAAGGGA+CGG + 50571:6216-6235 None:intergenic 40.0%
GACATACCTCCTGTACAAAA+TGG + 50571:6027-6046 None:intergenic 40.0%
GACATATCAAAGTGAGGTCT+AGG + 50571:5957-5976 None:intergenic 40.0%
GATTTGATTGATATGCAGCG+TGG + 50571:6300-6319 None:intergenic 40.0%
GGAACCGATGTCAATGAATT+GGG - 50571:6505-6524 MS.gene050725:CDS 40.0%
GGTTGAGACATATCAAAGTG+AGG + 50571:5963-5982 None:intergenic 40.0%
GTGCTTATAAGCAATGTAGG+TGG - 50571:6431-6450 MS.gene050725:intron 40.0%
GTGTCAAACTATCAGTTCAG+TGG + 50571:6248-6267 None:intergenic 40.0%
TTAGCTATAGTCTGAAAGGC+AGG + 50571:5984-6003 None:intergenic 40.0%
TTTGCACTCCCTAATGACAA+TGG - 50571:5915-5934 MS.gene050725:intron 40.0%
! CAATGAATTGGGGTGCTAAT+TGG - 50571:6516-6535 MS.gene050725:CDS 40.0%
! TCATTAGGGAGTGCAAAGTT+TGG + 50571:5912-5931 None:intergenic 40.0%
! TTGTTCCCATTTTGTACAGG+AGG - 50571:6018-6037 MS.gene050725:intron 40.0%
!! TGGGAGAGACTATTTTGAGT+TGG - 50571:6409-6428 MS.gene050725:intron 40.0%
AACTTGTAGGTGGTGCATGT+GGG - 50571:5709-5728 MS.gene050725:intron 45.0%
AAGTGATGCCTCAGGAACAA+TGG - 50571:5585-5604 MS.gene050725:CDS 45.0%
AGAGTTGGGTGCAAAAGAAG+TGG - 50571:6365-6384 MS.gene050725:intron 45.0%
AGTGATGCCTCAGGAACAAT+GGG - 50571:5586-5605 MS.gene050725:CDS 45.0%
ATAAGCAATGTAGGTGGAGG+TGG - 50571:6437-6456 MS.gene050725:intron 45.0%
ATGCAAGGCAAGTTCCTCAA+TGG - 50571:5838-5857 MS.gene050725:intron 45.0%
CACCAACCACCATTGTCATT+AGG + 50571:5927-5946 None:intergenic 45.0%
CCACGTTGTAAAATGACCAC+CGG + 50571:6183-6202 None:intergenic 45.0%
GCACCCCAATTCATTGACAT+CGG + 50571:6512-6531 None:intergenic 45.0%
GCTCATGCAACTTTCTATGG+TGG - 50571:5562-5581 MS.gene050725:CDS 45.0%
GGGAACCGATGTCAATGAAT+TGG - 50571:6504-6523 MS.gene050725:CDS 45.0%
TCCCTAATGACAATGGTGGT+TGG - 50571:5922-5941 MS.gene050725:intron 45.0%
! AATGATGGGAAGTCATGTGG+TGG - 50571:5795-5814 MS.gene050725:intron 45.0%
! TAACTTGTAGGTGGTGCATG+TGG - 50571:5708-5727 MS.gene050725:CDS 45.0%
! TTAGGGAGTGCAAAGTTTGG+TGG + 50571:5909-5928 None:intergenic 45.0%
!! CGGTGGTCATTTTACAACGT+GGG - 50571:6181-6200 MS.gene050725:CDS 45.0%
GCACTCCCTAATGACAATGG+TGG - 50571:5918-5937 MS.gene050725:intron 50.0%
GTTCCTCAATGGTGTCTCAG+AGG - 50571:5849-5868 MS.gene050725:intron 50.0%
! TATGGTGGAAGTGATGCCTC+AGG - 50571:5577-5596 MS.gene050725:CDS 50.0%
!! CCGGTGGTCATTTTACAACG+TGG - 50571:6180-6199 MS.gene050725:CDS 50.0%
GTAGGTGGTGCATGTGGGTA+TGG - 50571:5714-5733 MS.gene050725:intron 55.0%
!! GTGCCTCTGAGACACCATTG+AGG + 50571:5855-5874 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
50571 gene 5475 6619 5475 ID=MS.gene050725
50571 mRNA 5475 6619 5475 ID=MS.gene050725.t1;Parent=MS.gene050725
50571 exon 6487 6619 6487 ID=MS.gene050725.t1.exon1;Parent=MS.gene050725.t1
50571 CDS 6487 6619 6487 ID=cds.MS.gene050725.t1;Parent=MS.gene050725.t1
50571 exon 6055 6376 6055 ID=MS.gene050725.t1.exon2;Parent=MS.gene050725.t1
50571 CDS 6055 6376 6055 ID=cds.MS.gene050725.t1;Parent=MS.gene050725.t1
50571 exon 5475 5727 5475 ID=MS.gene050725.t1.exon3;Parent=MS.gene050725.t1
50571 CDS 5475 5727 5475 ID=cds.MS.gene050725.t1;Parent=MS.gene050725.t1
Gene Sequence

>MS.gene050725

ATGGAAAAAATAATTATTTGTGTTCTTATATTGCTGATAAATTTACTCACTAAAGAACTCAGAGTAGAATCTGTTGTTTGGCAGCAAGCTCATGCAACTTTCTATGGTGGAAGTGATGCCTCAGGAACAATGGGTAAATTGCTTCTTCTTTTGATTAACTTTATTCAAGTCTTAAGACAAATTAAAGTCTTAATCAAATTATGATATAGTTAAGCATATAACTTTATTTTGATTAACTTGTAGGTGGTGCATGTGGGTATGGAAATCTGTACACAGATGGATATGGTATAAAGTCAGCAGCATTGAGTACTGCTCTCTTTAATGATGGGAAGTCATGTGGTGGTTGCTATCAGATAGTTTGTGATGCAAGGCAAGTTCCTCAATGGTGTCTCAGAGGCACTTCCATCACTATTACTGCCACAAATTTTTGTCCACCAAACTTTGCACTCCCTAATGACAATGGTGGTTGGTGTAATCCCCCTAGACCTCACTTTGATATGTCTCAACCTGCCTTTCAGACTATAGCTAAGTACAGAGCTGGAATTGTTCCCATTTTGTACAGGAGGTATGTCTTAATTTGCTGATCAAACAATTAAATTGTTAATTAGAGTTTAATTTTGAAGCATGTTATTTTTTCAGTCAATTGATAAAAAATCGTTGATTTTTAAATTAACGAAAATAGAATAATTATTAATTAAAGTAACACCGGTGGTCATTTTACAACGTGGGATTTGAACTCCGTCCCTTGATGTTTCATTGTCAAACTTTTACCACTGAACTGATAGTTTGACACCTAGCGAACAAGTATTTTTAGTGGATACACCACGCTGCATATCAATCAAATCTATAAATATGTGTTTTAAGCAATTTTTTGTTGTATTTATATATGCAGAGTTGGGTGCAAAAGAAGTGGAAACATAAGATTTACCATAAATGGGAGAGACTATTTTGAGTTGGTGCTTATAAGCAATGTAGGTGGAGGTGGAGAGATTTCTAAAGTTTGGATAAAAGGGTCAAAAAAGAATAAATGGGAACCGATGTCAATGAATTGGGGTGCTAATTGGCAAAGCCTAAGCTATCTTAATGGTCAGAGCTTATCTTTTAGAATTCAACTCAAAAATGGGAAGGACTCGTACAGCTATTAA

Protein sequence

>MS.gene050725.t1

MEKIIICVLILLINLLTKELRVESVVWQQAHATFYGGSDASGTMGGACGYGNLYTDGYGIKSAALSTALFNDGKSCGGCYQIVCDARQVPQWCLRGTSITITATNFCPPNFALPNDNGGWCNPPRPHFDMSQPAFQTIAKYRAGIVPILYRRVGCKRSGNIRFTINGRDYFELVLISNVGGGGEISKVWIKGSKKNKWEPMSMNWGANWQSLSYLNGQSLSFRIQLKNGKDSYSY