AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45073

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45073.t1 MTR_5g034790 98.535 273 4 0 1 273 96 368 0.0 561
MS.gene45073.t1 MTR_8g063660 68.772 285 70 8 1 269 48 329 9.86e-126 361
MS.gene45073.t1 MTR_6g007737 57.471 261 97 6 1 254 113 366 1.19e-93 281
MS.gene45073.t1 MTR_4g106680 51.793 251 82 5 6 252 91 306 1.40e-74 230
MS.gene45073.t1 MTR_4g129420 39.385 325 118 12 1 253 97 414 4.42e-54 182
MS.gene45073.t1 MTR_3g100400 52.113 71 34 0 19 89 128 198 1.26e-22 96.7
MS.gene45073.t1 MTR_5g030010 31.839 223 131 6 11 212 112 334 4.76e-22 94.4
MS.gene45073.t1 MTR_5g064890 41.228 114 58 2 21 134 128 232 1.21e-21 93.2
MS.gene45073.t1 MTR_5g066250 51.948 77 34 1 16 89 126 202 4.13e-21 92.4
MS.gene45073.t1 MTR_7g073200 46.739 92 43 2 12 100 72 160 6.30e-21 87.8
MS.gene45073.t1 MTR_8g023400 46.835 79 41 1 14 92 85 162 7.84e-21 90.5
MS.gene45073.t1 MTR_8g089880 55.072 69 31 0 21 89 127 195 9.68e-21 89.4
MS.gene45073.t1 MTR_4g093830 49.383 81 41 0 12 92 117 197 1.11e-20 90.5
MS.gene45073.t1 MTR_1g074190 41.860 129 56 4 21 130 99 227 1.97e-20 89.7
MS.gene45073.t1 MTR_3g110190 50.704 71 35 0 19 89 64 134 2.95e-20 89.0
MS.gene45073.t1 MTR_3g094000 43.333 90 47 2 6 92 76 164 6.48e-20 84.7
MS.gene45073.t1 MTR_3g099650 44.872 78 40 1 21 95 107 184 7.48e-20 88.2
MS.gene45073.t1 MTR_2g009310 56.522 69 30 0 21 89 135 203 9.97e-20 88.2
MS.gene45073.t1 MTR_6g087150 47.945 73 38 0 20 92 92 164 1.47e-19 87.4
MS.gene45073.t1 MTR_8g066780 46.535 101 49 3 21 116 114 214 1.53e-19 87.4
MS.gene45073.t1 MTR_5g064990 49.333 75 35 1 21 92 114 188 2.86e-19 86.7
MS.gene45073.t1 MTR_6g055030 48.000 75 36 1 21 92 97 171 2.98e-19 85.9
MS.gene45073.t1 MTR_5g064980 41.121 107 54 2 16 113 103 209 3.26e-19 86.7
MS.gene45073.t1 MTR_1g100737 42.683 82 42 1 19 95 90 171 5.93e-19 82.8
MS.gene45073.t1 MTR_3g091620 50.000 72 36 0 21 92 101 172 6.47e-19 85.5
MS.gene45073.t1 MTR_1g070140 48.718 78 39 1 16 92 98 175 7.66e-19 85.1
MS.gene45073.t1 MTR_7g113800 46.835 79 37 2 21 95 91 168 1.02e-18 85.1
MS.gene45073.t1 MTR_1g043410 49.333 75 35 1 21 92 94 168 2.07e-18 83.6
MS.gene45073.t1 MTR_3g078450 37.736 106 62 2 8 111 63 166 2.17e-18 81.6
MS.gene45073.t1 MTR_7g116180 50.000 76 35 1 21 93 67 142 2.25e-18 80.9
MS.gene45073.t1 MTR_1g082550 48.101 79 36 2 21 95 98 175 2.67e-18 84.0
MS.gene45073.t1 MTR_7g116220 46.250 80 40 1 21 97 70 149 3.10e-18 80.5
MS.gene45073.t1 MTR_8g009190 33.333 162 74 4 24 182 79 209 3.37e-18 83.2
MS.gene45073.t1 MTR_1g070425 50.000 76 35 1 21 93 68 143 4.67e-18 80.1
MS.gene45073.t1 MTR_3g078470 30.435 161 96 4 8 160 63 215 6.53e-18 80.5
MS.gene45073.t1 MTR_4g030070 42.500 80 45 1 19 98 83 161 7.19e-18 80.5
MS.gene45073.t1 MTR_7g076250 34.513 113 69 3 19 129 61 170 2.00e-17 78.6
MS.gene45073.t1 MTR_7g117540 48.000 75 34 2 22 94 77 148 3.19e-17 80.1
MS.gene45073.t1 MTR_4g120270 40.594 101 56 2 21 121 80 176 4.70e-17 79.3
MS.gene45073.t1 MTR_2g090950 42.500 80 40 1 19 92 89 168 5.11e-17 78.6
MS.gene45073.t1 MTR_3g086730 34.416 154 83 6 22 162 47 195 6.10e-17 78.2
MS.gene45073.t1 MTR_3g086700 49.333 75 33 2 22 92 47 120 6.67e-17 77.0
MS.gene45073.t1 MTR_4g063070 42.553 94 43 2 7 89 285 378 7.02e-17 80.1
MS.gene45073.t1 MTR_2g044570 50.000 68 31 1 30 94 63 130 1.83e-16 77.8
MS.gene45073.t1 MTR_2g090905 38.889 90 37 2 13 90 31 114 1.84e-16 74.3
MS.gene45073.t1 MTR_2g055790 40.449 89 50 2 4 92 60 145 3.98e-16 75.1
MS.gene45073.t1 MTR_2g096350 46.250 80 41 1 16 93 102 181 4.24e-16 75.5
MS.gene45073.t1 MTR_5g024730 44.872 78 41 1 17 92 73 150 5.10e-16 75.1
MS.gene45073.t1 MTR_3g464780 41.772 79 42 1 18 92 99 177 6.40e-16 75.9
MS.gene45073.t1 MTR_2g035800 46.053 76 41 0 19 94 74 149 9.99e-16 73.2
MS.gene45073.t1 MTR_1g492830 42.466 73 41 1 21 92 61 133 1.83e-15 73.9
MS.gene45073.t1 MTR_7g116200 48.571 70 35 1 21 90 68 136 2.42e-15 72.4
MS.gene45073.t1 MTR_3g114500 43.038 79 42 1 19 97 46 121 2.62e-15 71.2
MS.gene45073.t1 MTR_3g071300 40.404 99 54 2 21 115 76 173 3.26e-15 72.0
MS.gene45073.t1 MTR_8g099495 48.611 72 35 1 21 90 319 390 3.52e-15 75.1
MS.gene45073.t1 MTR_4g056590 43.421 76 40 2 21 96 100 172 4.39e-15 72.4
MS.gene45073.t1 MTR_6g007680 44.595 74 41 0 22 95 41 114 5.72e-15 70.9
MS.gene45073.t1 MTR_8g028465 43.662 71 38 1 21 91 90 158 6.39e-15 72.0
MS.gene45073.t1 MTR_2g436100 46.377 69 37 0 21 89 93 161 8.51e-15 73.2
MS.gene45073.t1 MTR_8g027980 34.839 155 83 6 24 160 93 247 9.75e-15 72.8
MS.gene45073.t1 MTR_8g073120 42.466 73 40 1 19 89 68 140 1.02e-14 71.6
MS.gene45073.t1 MTR_3g095560 50.000 68 26 1 46 105 135 202 1.03e-14 72.0
MS.gene45073.t1 MTR_3g116000 45.333 75 40 1 19 93 77 150 1.04e-14 71.2
MS.gene45073.t1 MTR_7g096690 40.789 76 41 1 21 92 70 145 1.45e-14 72.8
MS.gene45073.t1 MTR_4g094725 44.595 74 39 1 19 90 76 149 1.47e-14 69.7
MS.gene45073.t1 MTR_5g076990 39.535 86 48 1 21 102 76 161 2.51e-14 72.0
MS.gene45073.t1 MTR_3g012550 39.726 73 42 1 21 91 80 152 2.54e-14 69.7
MS.gene45073.t1 MTR_7g116210 47.143 70 36 1 21 90 10 78 2.84e-14 68.2
MS.gene45073.t1 MTR_7g108855 45.714 70 36 1 22 89 67 136 3.85e-14 69.7
MS.gene45073.t1 MTR_8g092840 43.038 79 40 2 17 92 109 185 3.89e-14 70.5
MS.gene45073.t1 MTR_8g092960 42.857 77 39 2 19 92 101 175 4.59e-14 70.1
MS.gene45073.t1 MTR_8g032690 44.118 68 37 1 47 113 19 86 5.66e-14 67.0
MS.gene45073.t1 MTR_3g078120 48.529 68 33 1 30 95 66 133 5.88e-14 68.2
MS.gene45073.t1 MTR_7g079280 39.726 73 42 1 21 91 80 152 7.46e-14 68.6
MS.gene45073.t1 MTR_8g092930 44.000 75 37 2 21 92 112 184 9.55e-14 69.3
MS.gene45073.t1 MTR_8g092990 59.574 47 19 0 46 92 140 186 9.82e-14 69.3
MS.gene45073.t1 MTR_1g056350 33.645 107 64 2 11 112 64 168 1.08e-13 67.8
MS.gene45073.t1 MTR_8g092970 59.574 47 19 0 46 92 130 176 1.20e-13 68.9
MS.gene45073.t1 MTR_8g092985 42.308 78 40 2 19 93 109 184 1.29e-13 68.6
MS.gene45073.t1 MTR_7g079290 38.158 76 43 2 21 92 125 200 2.19e-13 67.8
MS.gene45073.t1 MTR_3g091200 39.189 74 43 1 20 91 302 375 2.96e-13 69.3
MS.gene45073.t1 MTR_6g055030 60.000 50 19 1 44 92 91 140 3.11e-13 68.6
MS.gene45073.t1 MTR_5g082940 39.189 74 43 1 21 92 75 148 3.99e-13 66.2
MS.gene45073.t1 MTR_5g026730 57.778 45 19 0 47 91 94 138 5.69e-13 65.9
MS.gene45073.t1 MTR_8g093010 40.260 77 41 2 19 92 105 179 6.85e-13 66.6
MS.gene45073.t1 MTR_8g074900 38.095 84 47 2 10 92 146 225 1.37e-12 65.9
MS.gene45073.t1 MTR_8g099515 44.048 84 42 2 21 99 309 392 1.52e-12 67.4
MS.gene45073.t1 MTR_8g071815 58.140 43 18 0 47 89 90 132 1.61e-12 64.7
MS.gene45073.t1 MTR_5g009200 44.444 72 38 1 21 90 322 393 2.35e-12 66.6
MS.gene45073.t1 MTR_5g009210 44.444 72 38 1 21 90 287 358 3.36e-12 66.2
MS.gene45073.t1 MTR_5g009190 43.056 72 39 1 21 90 266 337 3.75e-12 65.9
MS.gene45073.t1 MTR_8g066740 38.158 76 43 1 21 92 76 151 3.79e-12 65.5
MS.gene45073.t1 MTR_5g092790 35.870 92 56 2 2 90 137 228 4.47e-12 64.7
MS.gene45073.t1 MTR_5g022920 52.000 50 24 0 43 92 185 234 5.62e-12 64.3
MS.gene45073.t1 MTR_3g081030 41.026 78 45 1 22 99 95 171 6.14e-12 63.9
MS.gene45073.t1 MTR_5g092750 33.696 92 58 2 2 90 137 228 8.18e-12 63.9
MS.gene45073.t1 MTR_5g026720 53.333 45 21 0 47 91 93 137 1.24e-11 62.0
MS.gene45073.t1 MTR_5g071930 36.000 75 45 1 21 95 66 137 1.43e-11 62.4
MS.gene45073.t1 MTR_8g028815 46.774 62 32 1 30 90 75 136 1.77e-11 61.6
MS.gene45073.t1 MTR_5g026260 42.424 66 35 3 26 89 483 547 1.92e-11 64.3
MS.gene45073.t1 MTR_4g077570 49.020 51 22 2 42 89 6 55 2.18e-11 59.3
MS.gene45073.t1 MTR_1g090480 36.000 75 44 1 21 91 72 146 2.38e-11 61.2
MS.gene45073.t1 MTR_5g010100 50.000 50 24 1 47 95 81 130 2.74e-11 60.5
MS.gene45073.t1 MTR_6g007670 42.254 71 40 1 27 96 151 221 4.32e-11 62.0
MS.gene45073.t1 MTR_6g007670 50.000 52 26 0 45 96 31 82 5.14e-11 62.0
MS.gene45073.t1 MTR_1g026070 37.975 79 46 1 19 97 46 121 5.03e-11 59.7
MS.gene45073.t1 MTR_4g022410 55.319 47 21 0 46 92 112 158 5.58e-11 60.8
MS.gene45073.t1 MTR_8g098770 45.312 64 32 2 28 90 69 130 6.33e-11 60.1
MS.gene45073.t1 MTR_1g090460 39.706 68 35 2 28 90 72 138 7.56e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45073.t1 AT1G23980 61.905 252 72 6 1 247 98 330 4.26e-90 272
MS.gene45073.t1 AT5G40250 50.000 290 119 8 1 271 87 369 1.55e-84 258
MS.gene45073.t1 AT2G18650 43.214 280 121 11 6 251 84 359 8.30e-56 185
MS.gene45073.t1 AT3G48030 48.421 190 86 3 10 198 91 269 5.73e-54 176
MS.gene45073.t1 AT3G48030 48.421 190 86 3 10 198 169 347 3.23e-53 176
MS.gene45073.t1 AT4G30400 40.065 307 123 11 6 252 92 397 1.76e-52 177
MS.gene45073.t1 AT5G57750 62.500 88 32 1 4 90 78 165 4.14e-34 123
MS.gene45073.t1 AT5G05810 44.330 97 52 1 19 113 118 214 1.57e-22 96.3
MS.gene45073.t1 AT3G03550 52.564 78 37 0 12 89 124 201 2.09e-22 95.5
MS.gene45073.t1 AT2G20030 50.000 86 41 1 7 90 82 167 3.02e-22 95.1
MS.gene45073.t1 AT1G72220 57.534 73 31 0 17 89 147 219 4.07e-22 95.1
MS.gene45073.t1 AT4G09110 31.515 165 102 3 21 174 94 258 1.18e-21 92.4
MS.gene45073.t1 AT4G40070 38.686 137 67 5 21 149 95 222 1.25e-21 92.8
MS.gene45073.t1 AT5G17600 51.948 77 37 0 13 89 108 184 2.40e-21 92.4
MS.gene45073.t1 AT1G53820 47.436 78 40 1 17 94 91 167 5.36e-21 90.9
MS.gene45073.t1 AT1G35330 43.299 97 51 2 21 114 100 195 1.27e-20 90.1
MS.gene45073.t1 AT3G60220 32.335 167 81 5 25 188 92 229 1.80e-20 89.7
MS.gene45073.t1 AT4G28890 52.113 71 34 0 20 90 96 166 1.88e-20 90.5
MS.gene45073.t1 AT2G34990 35.172 145 82 4 21 160 67 204 4.36e-20 88.2
MS.gene45073.t1 AT3G16720 51.351 74 35 1 21 94 94 166 5.76e-20 87.8
MS.gene45073.t1 AT2G34990 35.172 145 82 4 21 160 89 226 5.82e-20 88.2
MS.gene45073.t1 AT2G47560 51.316 76 35 1 19 92 78 153 1.20e-19 85.5
MS.gene45073.t1 AT2G35000 42.553 94 50 2 2 92 88 180 1.61e-19 87.4
MS.gene45073.t1 AT3G10910 40.000 100 54 1 20 119 87 180 3.51e-19 83.2
MS.gene45073.t1 AT4G33565 42.697 89 51 0 12 100 182 270 4.52e-19 86.3
MS.gene45073.t1 AT4G09120 48.000 75 36 1 21 92 94 168 6.39e-19 85.5
MS.gene45073.t1 AT5G10380 45.570 79 43 0 11 89 99 177 1.55e-18 84.0
MS.gene45073.t1 AT2G42350 47.945 73 31 2 21 90 75 143 1.82e-18 82.0
MS.gene45073.t1 AT4G09130 45.333 75 38 1 21 92 91 165 1.88e-18 84.3
MS.gene45073.t1 AT1G72310 42.857 77 43 1 17 93 98 173 2.70e-18 83.6
MS.gene45073.t1 AT1G04360 45.556 90 41 3 19 100 99 188 3.70e-18 83.6
MS.gene45073.t1 AT1G22500 46.753 77 38 1 19 92 87 163 4.76e-18 83.2
MS.gene45073.t1 AT5G43420 41.111 90 39 1 17 92 94 183 5.39e-18 83.2
MS.gene45073.t1 AT4G15975 44.444 72 39 1 21 92 51 121 6.98e-18 80.9
MS.gene45073.t1 AT5G01880 46.575 73 39 0 20 92 78 150 1.21e-17 78.6
MS.gene45073.t1 AT1G72200 48.000 75 36 1 21 92 115 189 1.26e-17 82.4
MS.gene45073.t1 AT5G27420 45.570 79 38 2 21 95 95 172 2.10e-17 81.3
MS.gene45073.t1 AT2G42360 43.750 80 44 1 12 90 72 151 2.54e-17 79.3
MS.gene45073.t1 AT3G05200 38.542 96 54 2 4 95 82 176 2.96e-17 81.3
MS.gene45073.t1 AT5G05280 45.205 73 39 1 21 93 88 159 3.06e-17 77.8
MS.gene45073.t1 AT3G18930 46.341 82 34 1 21 92 120 201 4.48e-17 80.9
MS.gene45073.t1 AT3G18930 46.341 82 34 1 21 92 120 201 4.48e-17 80.9
MS.gene45073.t1 AT3G18930 46.341 82 34 1 21 92 120 201 4.48e-17 80.9
MS.gene45073.t1 AT3G62690 42.169 83 48 0 22 104 88 170 5.96e-17 78.6
MS.gene45073.t1 AT1G76410 47.368 76 39 1 19 93 75 150 2.91e-16 75.5
MS.gene45073.t1 AT4G10150 45.833 72 38 1 21 92 86 156 3.19e-16 76.3
MS.gene45073.t1 AT3G61550 37.931 87 43 1 16 91 94 180 3.66e-16 75.5
MS.gene45073.t1 AT4G09100 46.575 73 34 2 21 89 56 127 5.57e-16 73.2
MS.gene45073.t1 AT1G20823 46.053 76 40 1 19 93 82 157 7.06e-16 74.7
MS.gene45073.t1 AT2G35910 41.772 79 46 0 20 98 120 198 8.87e-16 74.7
MS.gene45073.t1 AT4G10160 41.333 75 43 1 21 95 72 145 1.99e-15 73.9
MS.gene45073.t1 AT2G27940 42.667 75 41 2 21 94 115 188 2.28e-15 73.9
MS.gene45073.t1 AT2G46160 40.506 79 39 1 21 91 104 182 2.55e-15 73.6
MS.gene45073.t1 AT2G46160 40.506 79 39 1 21 91 104 182 2.55e-15 73.6
MS.gene45073.t1 AT4G17905 51.389 72 33 2 20 89 126 197 3.10e-15 74.7
MS.gene45073.t1 AT4G17905 51.389 72 33 2 20 89 152 223 5.45e-15 74.3
MS.gene45073.t1 AT5G07040 38.356 73 43 1 21 91 66 138 5.61e-15 71.2
MS.gene45073.t1 AT1G33480 41.667 72 41 1 21 92 75 145 2.04e-14 71.6
MS.gene45073.t1 AT2G35420 30.818 159 100 4 19 175 75 225 2.34e-14 71.2
MS.gene45073.t1 AT5G46650 41.758 91 46 3 6 92 73 160 3.26e-14 71.6
MS.gene45073.t1 AT4G35480 56.250 48 21 0 46 93 112 159 4.50e-14 69.7
MS.gene45073.t1 AT3G14320 55.102 49 22 0 41 89 82 130 6.19e-14 69.3
MS.gene45073.t1 AT1G49230 55.319 47 21 0 46 92 130 176 8.79e-14 69.3
MS.gene45073.t1 AT1G49220 41.026 78 41 2 19 93 106 181 1.25e-13 69.3
MS.gene45073.t1 AT4G17920 41.558 77 42 1 19 92 79 155 1.73e-13 69.3
MS.gene45073.t1 AT5G58580 41.558 77 44 1 19 94 109 185 2.84e-13 68.9
MS.gene45073.t1 AT2G17450 53.191 47 22 0 46 92 101 147 4.30e-13 66.6
MS.gene45073.t1 AT3G18773 40.260 77 41 2 19 92 101 175 4.40e-13 67.4
MS.gene45073.t1 AT3G19140 35.632 87 52 2 20 103 53 138 4.83e-13 65.5
MS.gene45073.t1 AT1G49210 38.961 77 42 2 19 92 106 180 8.71e-13 66.6
MS.gene45073.t1 AT1G32361 38.889 72 42 1 21 92 130 199 1.64e-12 67.0
MS.gene45073.t1 AT5G53110 41.176 85 44 2 13 91 290 374 1.86e-12 67.0
MS.gene45073.t1 AT1G49200 36.364 77 44 2 19 92 107 181 2.93e-12 65.1
MS.gene45073.t1 AT5G47610 39.706 68 36 2 31 95 90 155 7.05e-12 62.8
MS.gene45073.t1 AT2G46493 48.889 45 23 0 47 91 138 182 9.07e-12 62.8
MS.gene45073.t1 AT1G28040 36.709 79 38 2 20 91 224 297 1.56e-11 63.9
MS.gene45073.t1 AT1G74410 32.468 77 52 0 16 92 145 221 2.42e-11 62.4
MS.gene45073.t1 AT4G17245 38.636 88 52 2 9 94 63 150 2.62e-11 61.2
MS.gene45073.t1 AT5G66070 49.020 51 26 0 42 92 171 221 2.98e-11 62.0
MS.gene45073.t1 AT5G66070 49.020 51 26 0 42 92 171 221 2.98e-11 62.0
MS.gene45073.t1 AT2G18670 42.857 70 39 1 21 89 83 152 3.15e-11 61.2
MS.gene45073.t1 AT4G30370 38.158 76 47 0 18 93 86 161 3.49e-11 61.2
MS.gene45073.t1 AT4G35840 36.000 100 52 3 1 92 141 236 3.64e-11 62.0
MS.gene45073.t1 AT5G06490 44.444 45 25 0 47 91 129 173 4.27e-11 61.2
MS.gene45073.t1 AT2G17730 53.488 43 20 0 47 89 196 238 4.76e-11 62.0
MS.gene45073.t1 AT5G66070 49.020 51 26 0 42 92 195 245 6.51e-11 61.6
MS.gene45073.t1 AT2G37580 51.111 45 22 0 46 90 141 185 8.27e-11 61.2
MS.gene45073.t1 AT2G46494 48.889 45 23 0 47 91 316 360 9.36e-11 62.0

Find 43 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAAAGCATGGTTACAATT+TGG 0.249185 5.3:+23764077 None:intergenic
TTCATTTACAAAGAGATAAT+TGG 0.261186 5.3:-23764170 MS.gene45073:CDS
AAGAAAACCCTTGTGAATAA+AGG 0.282232 5.3:+23763994 None:intergenic
ATCTCTTTGTAAATGAAAAC+TGG 0.313194 5.3:+23764176 None:intergenic
AAAGCTGTTGTAACTGTCTT+TGG 0.317238 5.3:+23764246 None:intergenic
CATCCATGGCAAACTTTCAT+TGG 0.319838 5.3:+23763488 None:intergenic
AGGGATGAGGAATTTGTTAT+AGG 0.323726 5.3:-23763936 MS.gene45073:CDS
GAGGAATTTGTTATAGGTAT+TGG 0.360199 5.3:-23763930 MS.gene45073:CDS
TGGTTCAGTTAACAAGGATA+TGG 0.378317 5.3:-23763910 MS.gene45073:CDS
ATCATATAATGAATGGGAAA+AGG 0.397814 5.3:-23763884 MS.gene45073:CDS
AGAAAACCCTTGTGAATAAA+GGG 0.412486 5.3:+23763995 None:intergenic
TAAAGGGTATTTCTACATAA+AGG 0.417585 5.3:+23764011 None:intergenic
ATGGAAAATCATATAATGAA+TGG 0.420938 5.3:-23763891 MS.gene45073:CDS
TCATATAATGAATGGGAAAA+GGG 0.428007 5.3:-23763883 MS.gene45073:CDS
AGAAGTTCAAATAATGAAGA+AGG 0.433060 5.3:-23763834 MS.gene45073:CDS
TGGAAAATCATATAATGAAT+GGG 0.440307 5.3:-23763890 MS.gene45073:CDS
GTTAGGAGATGTTATTCAAT+GGG 0.452527 5.3:-23763768 MS.gene45073:CDS
GAAGTTCAAATAATGAAGAA+GGG 0.466894 5.3:-23763833 MS.gene45073:CDS
AGTTGCTTTGAAAGCTTCAA+AGG 0.475384 5.3:-23763709 MS.gene45073:CDS
TGTTAGGAGATGTTATTCAA+TGG 0.476463 5.3:-23763769 MS.gene45073:CDS
AGCAACTTGCAAATCTGAAT+CGG 0.494580 5.3:+23763725 None:intergenic
AAACAAACAGCACAATCAAA+TGG 0.495898 5.3:+23764137 None:intergenic
GGAATTAGAGAAATTCAAGA+TGG 0.503159 5.3:-23763660 MS.gene45073:CDS
TCAAATGATGGTGATGTTGA+AGG 0.508703 5.3:-23763630 MS.gene45073:CDS
GTTTGAGGATGAAAGGGATG+AGG 0.511549 5.3:-23763949 MS.gene45073:CDS
CCTCTAGCTTTATTCATCCA+TGG 0.514626 5.3:+23763474 None:intergenic
ATATTAGTTGTATAGATACA+TGG 0.521360 5.3:-23764055 MS.gene45073:CDS
TGGTAGTATGAGACAATTGA+AGG 0.535499 5.3:-23763682 MS.gene45073:CDS
GCAAGAAGATTAACATTGCA+AGG 0.535706 5.3:-23763608 MS.gene45073:CDS
GTTGCTTTGAAAGCTTCAAA+GGG 0.545062 5.3:-23763708 MS.gene45073:CDS
GAATTAGAGAAATTCAAGAT+GGG 0.549355 5.3:-23763659 MS.gene45073:CDS
AGGTATTGGTTCAGTTAACA+AGG 0.561780 5.3:-23763916 MS.gene45073:CDS
AAGATTAACATTGCAAGGAA+AGG 0.566180 5.3:-23763603 MS.gene45073:CDS
CTGTTTCTAAGATATGGCAA+TGG 0.569913 5.3:-23763569 MS.gene45073:CDS
TTGAAAGCTTCAAAGGGTGA+TGG 0.572129 5.3:-23763702 MS.gene45073:CDS
GAAATACCCTTTATTCACAA+GGG 0.583195 5.3:-23764001 MS.gene45073:CDS
TACAACTAATATGAAAAGCA+TGG 0.605772 5.3:+23764066 None:intergenic
AAGTTCAAATAATGAAGAAG+GGG 0.611593 5.3:-23763832 MS.gene45073:CDS
AGAAATACCCTTTATTCACA+AGG 0.611996 5.3:-23764002 MS.gene45073:CDS
TTGCCAATGAAAGTTTGCCA+TGG 0.621500 5.3:-23763491 MS.gene45073:CDS
GGTAGTATGAGACAATTGAA+GGG 0.646173 5.3:-23763681 MS.gene45073:CDS
CCATGGATGAATAAAGCTAG+AGG 0.652428 5.3:-23763474 MS.gene45073:CDS
AGTTCAAATAATGAAGAAGG+GGG 0.682254 5.3:-23763831 MS.gene45073:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAGTTGTATAGATACA+TGG - chr5.3:23763678-23763697 MS.gene45073:CDS 20.0%
!! ATGGAAAATCATATAATGAA+TGG - chr5.3:23763842-23763861 MS.gene45073:CDS 20.0%
!! TGGAAAATCATATAATGAAT+GGG - chr5.3:23763843-23763862 MS.gene45073:CDS 20.0%
!! TTCATTTACAAAGAGATAAT+TGG - chr5.3:23763563-23763582 MS.gene45073:CDS 20.0%
!!! GTAATTGTAATTTTGATGTT+AGG - chr5.3:23763948-23763967 MS.gene45073:CDS 20.0%
! AAGTTCAAATAATGAAGAAG+GGG - chr5.3:23763901-23763920 MS.gene45073:CDS 25.0%
! AGAAGTTCAAATAATGAAGA+AGG - chr5.3:23763899-23763918 MS.gene45073:CDS 25.0%
! ATCATATAATGAATGGGAAA+AGG - chr5.3:23763849-23763868 MS.gene45073:CDS 25.0%
! ATCTCTTTGTAAATGAAAAC+TGG + chr5.3:23763560-23763579 None:intergenic 25.0%
! GAAGTTCAAATAATGAAGAA+GGG - chr5.3:23763900-23763919 MS.gene45073:CDS 25.0%
! GAATTAGAGAAATTCAAGAT+GGG - chr5.3:23764074-23764093 MS.gene45073:CDS 25.0%
! TACAACTAATATGAAAAGCA+TGG + chr5.3:23763670-23763689 None:intergenic 25.0%
! TCATATAATGAATGGGAAAA+GGG - chr5.3:23763850-23763869 MS.gene45073:CDS 25.0%
!! TGTTTGTTTATGTGAGTTTT+TGG - chr5.3:23763610-23763629 MS.gene45073:CDS 25.0%
!!! GTTTTTCTGTTTCTAAGATA+TGG - chr5.3:23764158-23764177 MS.gene45073:CDS 25.0%
!!! TAAAGGGTATTTCTACATAA+AGG + chr5.3:23763725-23763744 None:intergenic 25.0%
AAACAAACAGCACAATCAAA+TGG + chr5.3:23763599-23763618 None:intergenic 30.0%
AAGAAAACCCTTGTGAATAA+AGG + chr5.3:23763742-23763761 None:intergenic 30.0%
AAGATTAACATTGCAAGGAA+AGG - chr5.3:23764130-23764149 MS.gene45073:CDS 30.0%
AGAAAACCCTTGTGAATAAA+GGG + chr5.3:23763741-23763760 None:intergenic 30.0%
AGAAATACCCTTTATTCACA+AGG - chr5.3:23763731-23763750 MS.gene45073:CDS 30.0%
AGTTCAAATAATGAAGAAGG+GGG - chr5.3:23763902-23763921 MS.gene45073:CDS 30.0%
GAAATACCCTTTATTCACAA+GGG - chr5.3:23763732-23763751 MS.gene45073:CDS 30.0%
GGAATTAGAGAAATTCAAGA+TGG - chr5.3:23764073-23764092 MS.gene45073:CDS 30.0%
GTTAGGAGATGTTATTCAAT+GGG - chr5.3:23763965-23763984 MS.gene45073:CDS 30.0%
TGTTAGGAGATGTTATTCAA+TGG - chr5.3:23763964-23763983 MS.gene45073:CDS 30.0%
! CTTTTCAATCTTCATGATTC+AGG - chr5.3:23763506-23763525 MS.gene45073:CDS 30.0%
! TGAAAAGCATGGTTACAATT+TGG + chr5.3:23763659-23763678 None:intergenic 30.0%
!! GAGGAATTTGTTATAGGTAT+TGG - chr5.3:23763803-23763822 MS.gene45073:CDS 30.0%
!!! AAATCCTGTTTTTGAGTTTG+AGG - chr5.3:23763769-23763788 MS.gene45073:CDS 30.0%
!!! TTTTGAGTTTGAGGATGAAA+GGG - chr5.3:23763778-23763797 MS.gene45073:CDS 30.0%
!!! TTTTTGAGTTTGAGGATGAA+AGG - chr5.3:23763777-23763796 MS.gene45073:CDS 30.0%
AAAGCTGTTGTAACTGTCTT+TGG + chr5.3:23763490-23763509 None:intergenic 35.0%
AGCAACTTGCAAATCTGAAT+CGG + chr5.3:23764011-23764030 None:intergenic 35.0%
GCAAGAAGATTAACATTGCA+AGG - chr5.3:23764125-23764144 MS.gene45073:CDS 35.0%
GGTAGTATGAGACAATTGAA+GGG - chr5.3:23764052-23764071 MS.gene45073:CDS 35.0%
TCATCCTCAAACTCAAAAAC+AGG + chr5.3:23763776-23763795 None:intergenic 35.0%
TGGTAGTATGAGACAATTGA+AGG - chr5.3:23764051-23764070 MS.gene45073:CDS 35.0%
TGGTTCAGTTAACAAGGATA+TGG - chr5.3:23763823-23763842 MS.gene45073:CDS 35.0%
! TCAAATGATGGTGATGTTGA+AGG - chr5.3:23764103-23764122 MS.gene45073:CDS 35.0%
!! AGGGATGAGGAATTTGTTAT+AGG - chr5.3:23763797-23763816 MS.gene45073:CDS 35.0%
!! AGGTATTGGTTCAGTTAACA+AGG - chr5.3:23763817-23763836 MS.gene45073:CDS 35.0%
!! AGTTGCTTTGAAAGCTTCAA+AGG - chr5.3:23764024-23764043 MS.gene45073:CDS 35.0%
!! CTGTTTCTAAGATATGGCAA+TGG - chr5.3:23764164-23764183 MS.gene45073:CDS 35.0%
!! GATGGGAGTTTTTCAAATGA+TGG - chr5.3:23764091-23764110 MS.gene45073:CDS 35.0%
!! GTTGCTTTGAAAGCTTCAAA+GGG - chr5.3:23764025-23764044 MS.gene45073:CDS 35.0%
!!! GGAAAAGGGTTTTTTCTGTT+AGG - chr5.3:23763864-23763883 MS.gene45073:CDS 35.0%
CATCCATGGCAAACTTTCAT+TGG + chr5.3:23764248-23764267 None:intergenic 40.0%
CCATGGATGAATAAAGCTAG+AGG - chr5.3:23764259-23764278 MS.gene45073:CDS 40.0%
TTGAAAGCTTCAAAGGGTGA+TGG - chr5.3:23764031-23764050 MS.gene45073:CDS 40.0%
TTGCCAATGAAAGTTTGCCA+TGG - chr5.3:23764242-23764261 MS.gene45073:CDS 40.0%
!! CCTCTAGCTTTATTCATCCA+TGG + chr5.3:23764262-23764281 None:intergenic 40.0%
!!! AGGGTTTTTTCTGTTAGGCT+TGG - chr5.3:23763869-23763888 MS.gene45073:CDS 40.0%
GTTTGAGGATGAAAGGGATG+AGG - chr5.3:23763784-23763803 MS.gene45073:CDS 45.0%
! GAAGGGGGTGCAAAAAGTGA+AGG - chr5.3:23763917-23763936 MS.gene45073:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 23763467 23764288 23763467 ID=MS.gene45073
chr5.3 mRNA 23763467 23764288 23763467 ID=MS.gene45073.t1;Parent=MS.gene45073
chr5.3 exon 23763467 23764288 23763467 ID=MS.gene45073.t1.exon1;Parent=MS.gene45073.t1
chr5.3 CDS 23763467 23764288 23763467 ID=cds.MS.gene45073.t1;Parent=MS.gene45073.t1
Gene Sequence

>MS.gene45073

ATGTCTGAATCTGATGCATACCAAAGACAGTTACAACAGCTTTTCAATCTTCATGATTCAGGTTTAGATCAAGCATTCATTGATGCACTTCCAGTTTTCATTTACAAAGAGATAATTGGTTTGAAAGAACCATTTGATTGTGCTGTTTGTTTATGTGAGTTTTTGGAACAAGACAAGTTAAGATTGTTACCAAATTGTAACCATGCTTTTCATATTAGTTGTATAGATACATGGTTATTGTCAAATTCATCTTGTCCTTTATGTAGAAATACCCTTTATTCACAAGGGTTTTCTTTTGATAAAAATCCTGTTTTTGAGTTTGAGGATGAAAGGGATGAGGAATTTGTTATAGGTATTGGTTCAGTTAACAAGGATATGGAAAATCATATAATGAATGGGAAAAGGGTTTTTTCTGTTAGGCTTGGAAAGTTTAGAAGTTCAAATAATGAAGAAGGGGGTGCAAAAAGTGAAGGTGAGAGCAGTAATTGTAATTTTGATGTTAGGAGATGTTATTCAATGGGATCTTTTCAATATGTTGTTGCCGATTCAGATTTGCAAGTTGCTTTGAAAGCTTCAAAGGGTGATGGTAGTATGAGACAATTGAAGGGAATTAGAGAAATTCAAGATGGGAGTTTTTCAAATGATGGTGATGTTGAAGGCAAGAAGATTAACATTGCAAGGAAAGGTGAAAGTTTTTCTGTTTCTAAGATATGGCAATGGTCTAAGAAAGATAGTAAGATGCAAAGTTCATCTGATACACATTTTCATAATTGTGTTGCCAATGAAAGTTTGCCATGGATGAATAAAGCTAGAGGTACATGA
Protein sequence

>MS.gene45073.t1

MSESDAYQRQLQQLFNLHDSGLDQAFIDALPVFIYKEIIGLKEPFDCAVCLCEFLEQDKLRLLPNCNHAFHISCIDTWLLSNSSCPLCRNTLYSQGFSFDKNPVFEFEDERDEEFVIGIGSVNKDMENHIMNGKRVFSVRLGKFRSSNNEEGGAKSEGESSNCNFDVRRCYSMGSFQYVVADSDLQVALKASKGDGSMRQLKGIREIQDGSFSNDGDVEGKKINIARKGESFSVSKIWQWSKKDSKMQSSSDTHFHNCVANESLPWMNKARGT