AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052202


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052202.t1 MTR_3g087190 97.596 208 5 0 1 208 1 208 1.44e-149 414
MS.gene052202.t1 MTR_3g079680 66.026 156 21 3 16 170 27 151 9.14e-59 182
MS.gene052202.t1 MTR_8g090225 56.790 81 33 2 20 99 13 92 8.58e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052202.t1 AT4G39880 72.727 132 34 1 1 132 1 130 1.69e-61 190

Find 68 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAATCTGTTTGCGAGTTC+TGG 0.270288 3.2:-74442663 MS.gene052202:CDS
CGTGGGTTTAAGCCGCAAAA+TGG 0.274219 3.2:-74442717 MS.gene052202:CDS
TGCGAGTTCTGGTTCGAATC+AGG 0.295567 3.2:-74442652 MS.gene052202:CDS
TGCAAAACCTGATTACAAAA+AGG 0.295568 3.2:-74442901 MS.gene052202:CDS
AGCCGCAAAATGGGTATTAC+AGG 0.309367 3.2:-74442707 MS.gene052202:CDS
AACACGCTTGATTTCAATCT+TGG 0.311058 3.2:+74443010 None:intergenic
CAGGGACGGTTTGACCGTTC+TGG 0.311213 3.2:-74442633 MS.gene052202:CDS
GATTGAAATCAAGCGTGTTC+TGG 0.327858 3.2:-74443006 MS.gene052202:CDS
TAAGAAGAGTTTGAATAAAC+AGG 0.389935 3.2:-74442808 MS.gene052202:CDS
AGGGGAGGGTTTGATCGTTC+TGG 0.399564 3.2:-74442687 MS.gene052202:CDS
TTGAACGCCCTGGTTCGAAC+CGG 0.408564 3.2:-74442593 MS.gene052202:CDS
TTGACCGTTCTGGTTCGAAC+CGG 0.419451 3.2:-74442623 MS.gene052202:CDS
ATTTCAATCTTGGAAGCTGA+TGG 0.434077 3.2:+74443020 None:intergenic
CCATCAGCCCGGTTCGAACC+AGG 0.437888 3.2:+74442585 None:intergenic
AAGCGCCGGGGAAGAGTCAC+TGG 0.439330 3.2:-74442761 MS.gene052202:CDS
GCGAGTTCTGGTTCGAATCA+GGG 0.449121 3.2:-74442651 MS.gene052202:CDS
TGTATGTCGGTGAAGGTTGT+AGG 0.452936 3.2:+74443065 None:intergenic
GTAGGCAGGAGAAGCTTGAT+TGG 0.456166 3.2:+74443083 None:intergenic
ACCAGTCCTCATGTTACTCC+AGG 0.456222 3.2:+74442545 None:intergenic
GTTCTGCTGCAAAGTTTCCC+TGG 0.467776 3.2:-74442563 MS.gene052202:CDS
TGACCGTTCTGGTTCGAACC+GGG 0.469581 3.2:-74442622 MS.gene052202:CDS
TCCTCTTCAATCTCTCGAAT+CGG 0.471084 3.2:+74442831 None:intergenic
AGTATTGTTGAGGAAGCGCC+GGG 0.487009 3.2:-74442774 MS.gene052202:CDS
CCTGGTTCGAACCGGGCTGA+TGG 0.493822 3.2:-74442585 MS.gene052202:CDS
TGAACGCCCTGGTTCGAACC+GGG 0.496297 3.2:-74442592 MS.gene052202:CDS
GCAGGAGAAGCTTGATTGGT+AGG 0.500966 3.2:+74443087 None:intergenic
GATTCGGGTTAATCAAAAGC+GGG 0.502463 3.2:+74442859 None:intergenic
GTGGGTTTAAGCCGCAAAAT+GGG 0.507177 3.2:-74442716 MS.gene052202:CDS
CTACGGATTCGATGTCGAAA+AGG 0.508089 3.2:-74442976 MS.gene052202:CDS
AATCTTGGAAGCTGATGGAA+TGG 0.521693 3.2:+74443025 None:intergenic
CGGGGAAGGTTTGAACGCCC+TGG 0.529081 3.2:-74442603 MS.gene052202:CDS
CTGGTAGGAGTAATGCTACT+AGG 0.531882 3.2:-74442527 MS.gene052202:CDS
GAGTTTGAATAAACAGGCTA+TGG 0.532603 3.2:-74442802 MS.gene052202:CDS
TTCGACATCGAATCCGTAGA+GGG 0.537808 3.2:+74442980 None:intergenic
CAGGGCGTTCAAACCTTCCC+CGG 0.539251 3.2:+74442604 None:intergenic
CCCTGGAGTAACATGAGGAC+TGG 0.545437 3.2:-74442546 MS.gene052202:CDS
TAGTATTGTTGAGGAAGCGC+CGG 0.547147 3.2:-74442775 MS.gene052202:CDS
CGTGTTCTGGAATCCCTCTA+CGG 0.549464 3.2:-74442993 MS.gene052202:CDS
GTTCTGGTTCGAACCGGGGA+AGG 0.563380 3.2:-74442617 MS.gene052202:CDS
GATTTCATGTATGTCGGTGA+AGG 0.563490 3.2:+74443058 None:intergenic
TGTCGGTGAAGGTTGTAGGC+AGG 0.564653 3.2:+74443069 None:intergenic
GGAGTAACATGAGGACTGGT+AGG 0.575261 3.2:-74442542 MS.gene052202:CDS
CATCAGCCCGGTTCGAACCA+GGG 0.586597 3.2:+74442586 None:intergenic
AAAATGGGTATTACAGGGGA+GGG 0.587136 3.2:-74442701 MS.gene052202:CDS
GAGAGCGATTTCATGTATGT+CGG 0.587586 3.2:+74443052 None:intergenic
GCCGCAAAATGGGTATTACA+GGG 0.593489 3.2:-74442706 MS.gene052202:CDS
TCAAGCCAGTGACTCTTCCC+CGG 0.597800 3.2:+74442756 None:intergenic
GACCGTTCTGGTTCGAACCG+GGG 0.600430 3.2:-74442621 MS.gene052202:CDS
TCCGATTCGAGAGATTGAAG+AGG 0.601664 3.2:-74442832 MS.gene052202:CDS
CAAAATGGGTATTACAGGGG+AGG 0.606255 3.2:-74442702 MS.gene052202:CDS
TTTGAATAAACAGGCTATGG+CGG 0.615531 3.2:-74442799 MS.gene052202:CDS
GTTCTGGTTCGAATCAGGGA+CGG 0.619690 3.2:-74442647 MS.gene052202:CDS
TTGCAGCAGAACCATCAGCC+CGG 0.622044 3.2:+74442574 None:intergenic
GTTCGAACATTGAACATGGA+CGG 0.633586 3.2:-74442954 MS.gene052202:CDS
TTTCGACATCGAATCCGTAG+AGG 0.637992 3.2:+74442979 None:intergenic
CCAGTCCTCATGTTACTCCA+GGG 0.639983 3.2:+74442546 None:intergenic
GATGAGAATAAGAACACCCG+TGG 0.645135 3.2:-74442735 MS.gene052202:CDS
AAAGGTTCGAACATTGAACA+TGG 0.645998 3.2:-74442958 MS.gene052202:CDS
TATGGCGGCTAGTATTGTTG+AGG 0.660904 3.2:-74442784 MS.gene052202:CDS
AGTTTCCCTGGAGTAACATG+AGG 0.671157 3.2:-74442551 MS.gene052202:CDS
ATTCGGGTTAATCAAAAGCG+GGG 0.673297 3.2:+74442860 None:intergenic
TTCCCCGGTTCGAACCAGAA+CGG 0.681205 3.2:+74442619 None:intergenic
AGATTCGGGTTAATCAAAAG+CGG 0.681647 3.2:+74442858 None:intergenic
ATGAGAATAAGAACACCCGT+GGG 0.707036 3.2:-74442734 MS.gene052202:CDS
CCGCAAAATGGGTATTACAG+GGG 0.729401 3.2:-74442705 MS.gene052202:CDS
GACGGAAAGAAGAAGAAACG+TGG 0.737712 3.2:-74442936 MS.gene052202:CDS
GGAAAGAAGAAGAAACGTGG+CGG 0.756525 3.2:-74442933 MS.gene052202:CDS
GTATTGTTGAGGAAGCGCCG+GGG 0.806884 3.2:-74442773 MS.gene052202:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAGAAGAGTTTGAATAAAC+AGG - chr3.2:74442844-74442863 MS.gene052202:CDS 25.0%
TGCAAAACCTGATTACAAAA+AGG - chr3.2:74442751-74442770 MS.gene052202:CDS 30.0%
! ACAAAAGCCTTTTTGTAATC+AGG + chr3.2:74442761-74442780 None:intergenic 30.0%
AAAGGTTCGAACATTGAACA+TGG - chr3.2:74442694-74442713 MS.gene052202:CDS 35.0%
AACACGCTTGATTTCAATCT+TGG + chr3.2:74442645-74442664 None:intergenic 35.0%
ATTTCAATCTTGGAAGCTGA+TGG + chr3.2:74442635-74442654 None:intergenic 35.0%
GAGTTTGAATAAACAGGCTA+TGG - chr3.2:74442850-74442869 MS.gene052202:CDS 35.0%
TCTCGAATCGGAAAAAGATT+CGG + chr3.2:74442812-74442831 None:intergenic 35.0%
TTTGAATAAACAGGCTATGG+CGG - chr3.2:74442853-74442872 MS.gene052202:CDS 35.0%
! AGATTCGGGTTAATCAAAAG+CGG + chr3.2:74442797-74442816 None:intergenic 35.0%
AAAATGGGTATTACAGGGGA+GGG - chr3.2:74442951-74442970 MS.gene052202:CDS 40.0%
AATCTTGGAAGCTGATGGAA+TGG + chr3.2:74442630-74442649 None:intergenic 40.0%
ATGAGAATAAGAACACCCGT+GGG - chr3.2:74442918-74442937 MS.gene052202:CDS 40.0%
CTCGAATCGGAAAAAGATTC+GGG + chr3.2:74442811-74442830 None:intergenic 40.0%
GAGAGCGATTTCATGTATGT+CGG + chr3.2:74442603-74442622 None:intergenic 40.0%
GATTGAAATCAAGCGTGTTC+TGG - chr3.2:74442646-74442665 MS.gene052202:CDS 40.0%
GATTTCATGTATGTCGGTGA+AGG + chr3.2:74442597-74442616 None:intergenic 40.0%
GTTCGAACATTGAACATGGA+CGG - chr3.2:74442698-74442717 MS.gene052202:CDS 40.0%
TCCTCTTCAATCTCTCGAAT+CGG + chr3.2:74442824-74442843 None:intergenic 40.0%
! ATTCGGGTTAATCAAAAGCG+GGG + chr3.2:74442795-74442814 None:intergenic 40.0%
! GATTCGGGTTAATCAAAAGC+GGG + chr3.2:74442796-74442815 None:intergenic 40.0%
AGCCGCAAAATGGGTATTAC+AGG - chr3.2:74442945-74442964 MS.gene052202:CDS 45.0%
AGTTTCCCTGGAGTAACATG+AGG - chr3.2:74443101-74443120 MS.gene052202:CDS 45.0%
CAAAATGGGTATTACAGGGG+AGG - chr3.2:74442950-74442969 MS.gene052202:CDS 45.0%
CCGCAAAATGGGTATTACAG+GGG - chr3.2:74442947-74442966 MS.gene052202:CDS 45.0%
CTACGGATTCGATGTCGAAA+AGG - chr3.2:74442676-74442695 MS.gene052202:CDS 45.0%
CTGGTAGGAGTAATGCTACT+AGG - chr3.2:74443125-74443144 MS.gene052202:CDS 45.0%
GACGGAAAGAAGAAGAAACG+TGG - chr3.2:74442716-74442735 MS.gene052202:CDS 45.0%
GATGAGAATAAGAACACCCG+TGG - chr3.2:74442917-74442936 MS.gene052202:CDS 45.0%
GCCGCAAAATGGGTATTACA+GGG - chr3.2:74442946-74442965 MS.gene052202:CDS 45.0%
GGAAAGAAGAAGAAACGTGG+CGG - chr3.2:74442719-74442738 MS.gene052202:CDS 45.0%
GTGGGTTTAAGCCGCAAAAT+GGG - chr3.2:74442936-74442955 MS.gene052202:CDS 45.0%
TATGGCGGCTAGTATTGTTG+AGG - chr3.2:74442868-74442887 MS.gene052202:CDS 45.0%
TCGAATCTGTTTGCGAGTTC+TGG - chr3.2:74442989-74443008 MS.gene052202:CDS 45.0%
TGTATGTCGGTGAAGGTTGT+AGG + chr3.2:74442590-74442609 None:intergenic 45.0%
TTCGACATCGAATCCGTAGA+GGG + chr3.2:74442675-74442694 None:intergenic 45.0%
TTTCGACATCGAATCCGTAG+AGG + chr3.2:74442676-74442695 None:intergenic 45.0%
! ATTTTGCGGCTTAAACCCAC+GGG + chr3.2:74442936-74442955 None:intergenic 45.0%
! CATTTTGCGGCTTAAACCCA+CGG + chr3.2:74442937-74442956 None:intergenic 45.0%
! CCCCTGTAATACCCATTTTG+CGG + chr3.2:74442950-74442969 None:intergenic 45.0%
! TAGTATTGTTGAGGAAGCGC+CGG - chr3.2:74442877-74442896 MS.gene052202:CDS 45.0%
! TCCGATTCGAGAGATTGAAG+AGG - chr3.2:74442820-74442839 MS.gene052202:CDS 45.0%
ACCAGTCCTCATGTTACTCC+AGG + chr3.2:74443110-74443129 None:intergenic 50.0%
CCAGTCCTCATGTTACTCCA+GGG + chr3.2:74443109-74443128 None:intergenic 50.0%
CGTGGGTTTAAGCCGCAAAA+TGG - chr3.2:74442935-74442954 MS.gene052202:CDS 50.0%
GCAGGAGAAGCTTGATTGGT+AGG + chr3.2:74442568-74442587 None:intergenic 50.0%
GTAGGCAGGAGAAGCTTGAT+TGG + chr3.2:74442572-74442591 None:intergenic 50.0%
GTTCTGCTGCAAAGTTTCCC+TGG - chr3.2:74443089-74443108 MS.gene052202:CDS 50.0%
! GGAGTAACATGAGGACTGGT+AGG - chr3.2:74443110-74443129 MS.gene052202:CDS 50.0%
!! AGTATTGTTGAGGAAGCGCC+GGG - chr3.2:74442878-74442897 MS.gene052202:CDS 50.0%
!! CGTGTTCTGGAATCCCTCTA+CGG - chr3.2:74442659-74442678 MS.gene052202:CDS 50.0%
!! GCGAGTTCTGGTTCGAATCA+GGG - chr3.2:74443001-74443020 MS.gene052202:CDS 50.0%
!! GTTCTGGTTCGAATCAGGGA+CGG - chr3.2:74443005-74443024 MS.gene052202:CDS 50.0%
!! TGCGAGTTCTGGTTCGAATC+AGG - chr3.2:74443000-74443019 MS.gene052202:CDS 50.0%
!! TTGACCGTTCTGGTTCGAAC+CGG - chr3.2:74443029-74443048 MS.gene052202:CDS 50.0%
AGGGGAGGGTTTGATCGTTC+TGG - chr3.2:74442965-74442984 MS.gene052202:CDS 55.0%
CCCTGGAGTAACATGAGGAC+TGG - chr3.2:74443106-74443125 MS.gene052202:CDS 55.0%
TCAAGCCAGTGACTCTTCCC+CGG + chr3.2:74442899-74442918 None:intergenic 55.0%
TGTCGGTGAAGGTTGTAGGC+AGG + chr3.2:74442586-74442605 None:intergenic 55.0%
TTGCAGCAGAACCATCAGCC+CGG + chr3.2:74443081-74443100 None:intergenic 55.0%
! TTCCCCGGTTCGAACCAGAA+CGG + chr3.2:74443036-74443055 None:intergenic 55.0%
! TTGAACGCCCTGGTTCGAAC+CGG - chr3.2:74443059-74443078 MS.gene052202:CDS 55.0%
!! GTATTGTTGAGGAAGCGCCG+GGG - chr3.2:74442879-74442898 MS.gene052202:CDS 55.0%
!! TGACCGTTCTGGTTCGAACC+GGG - chr3.2:74443030-74443049 MS.gene052202:CDS 55.0%
CAGGGACGGTTTGACCGTTC+TGG - chr3.2:74443019-74443038 MS.gene052202:CDS 60.0%
! CAGGGCGTTCAAACCTTCCC+CGG + chr3.2:74443051-74443070 None:intergenic 60.0%
! CATCAGCCCGGTTCGAACCA+GGG + chr3.2:74443069-74443088 None:intergenic 60.0%
! TGAACGCCCTGGTTCGAACC+GGG - chr3.2:74443060-74443079 MS.gene052202:CDS 60.0%
!! GACCGTTCTGGTTCGAACCG+GGG - chr3.2:74443031-74443050 MS.gene052202:CDS 60.0%
!! GTTCTGGTTCGAACCGGGGA+AGG - chr3.2:74443035-74443054 MS.gene052202:CDS 60.0%
CGGGGAAGGTTTGAACGCCC+TGG - chr3.2:74443049-74443068 MS.gene052202:CDS 65.0%
! CCATCAGCCCGGTTCGAACC+AGG + chr3.2:74443070-74443089 None:intergenic 65.0%
! CCTGGTTCGAACCGGGCTGA+TGG - chr3.2:74443067-74443086 MS.gene052202:CDS 65.0%
!! AAGCGCCGGGGAAGAGTCAC+TGG - chr3.2:74442891-74442910 MS.gene052202:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 74442524 74443150 74442524 ID=MS.gene052202
chr3.2 mRNA 74442524 74443150 74442524 ID=MS.gene052202.t1;Parent=MS.gene052202
chr3.2 exon 74442524 74443150 74442524 ID=MS.gene052202.t1.exon1;Parent=MS.gene052202.t1
chr3.2 CDS 74442524 74443150 74442524 ID=cds.MS.gene052202.t1;Parent=MS.gene052202.t1
Gene Sequence

>MS.gene052202

ATGGGAAGCAGACTAGGAAGAAGAGTAGTGTACTTCGCAAACCTACCAATCAAGCTTCTCCTGCCTACAACCTTCACCGACATACATGAAATCGCTCTCAAAACCATTCCATCAGCTTCCAAGATTGAAATCAAGCGTGTTCTGGAATCCCTCTACGGATTCGATGTCGAAAAGGTTCGAACATTGAACATGGACGGAAAGAAGAAGAAACGTGGCGGAATCGTGATTGCAAAACCTGATTACAAAAAGGCTTTTGTAACGTTAAAAACCCCGCTTTTGATTAACCCGAATCTTTTTCCGATTCGAGAGATTGAAGAGGATAAGAAGAGTTTGAATAAACAGGCTATGGCGGCTAGTATTGTTGAGGAAGCGCCGGGGAAGAGTCACTGGCTTGATGAGAATAAGAACACCCGTGGGTTTAAGCCGCAAAATGGGTATTACAGGGGAGGGTTTGATCGTTCTGGTTCGAATCTGTTTGCGAGTTCTGGTTCGAATCAGGGACGGTTTGACCGTTCTGGTTCGAACCGGGGAAGGTTTGAACGCCCTGGTTCGAACCGGGCTGATGGTTCTGCTGCAAAGTTTCCCTGGAGTAACATGAGGACTGGTAGGAGTAATGCTACTAGGTAG

Protein sequence

>MS.gene052202.t1

MGSRLGRRVVYFANLPIKLLLPTTFTDIHEIALKTIPSASKIEIKRVLESLYGFDVEKVRTLNMDGKKKKRGGIVIAKPDYKKAFVTLKTPLLINPNLFPIREIEEDKKSLNKQAMAASIVEEAPGKSHWLDENKNTRGFKPQNGYYRGGFDRSGSNLFASSGSNQGRFDRSGSNRGRFERPGSNRADGSAAKFPWSNMRTGRSNATR