AlfalfaGEDB | MS.gene45590

M. sativa cultivar XinJiangDaYe / MS.gene45590

KEGG: K15200
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45590.t1	XP_013468026.1	97.4	78	2	0	1	78	76	153	1.10E-35	158.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45590.t1	A0A072VKV4	97.4	78	2	0	1	78	76	153	8.1e-36	158.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene45590	0.805792	1.13E-49	-1.69E-46
MS.gene049159	MS.gene45590	0.822763	1.99E-53	-1.69E-46
MS.gene051247	MS.gene45590	0.80024	1.58E-48	-1.69E-46
MS.gene052020	MS.gene45590	0.800888	1.17E-48	-1.69E-46
MS.gene052863	MS.gene45590	0.816561	5.19E-52	-1.69E-46
MS.gene055818	MS.gene45590	0.826981	2.01E-54	-1.69E-46
MS.gene057598	MS.gene45590	0.800247	1.58E-48	-1.69E-46
MS.gene057855	MS.gene45590	0.811969	5.37E-51	-1.69E-46
MS.gene057869	MS.gene45590	0.804424	2.17E-49	-1.69E-46
MS.gene058010	MS.gene45590	0.827203	1.78E-54	-1.69E-46
MS.gene058919	MS.gene45590	0.804231	2.39E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45590.t1	MTR_1g060580	97.436	78	2	0	1	78	76	153	5.01e-48	162

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 12 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTAGGTTTACTTACGGAAT+TGG	0.296469	3.4:-29893386	MS.gene45590:CDS
AATATCCATCTAAAAGCATT+AGG	0.316447	3.4:-29893403	MS.gene45590:CDS
TTAGGTTTACTTACGGAATT+GGG	0.426191	3.4:-29893385	MS.gene45590:CDS
TTCATCTGCCACTGTTCCTA+AGG	0.455578	3.4:-29893306	MS.gene45590:intron
AAAAGCATTAGGTTTACTTA+CGG	0.463447	3.4:-29893392	MS.gene45590:CDS
TTAAATGTGCAGAGAATGGA+AGG	0.560081	3.4:-29893432	MS.gene45590:intron
ACACCATTGCTAAACTCTGT+GGG	0.562879	3.4:-29894054	MS.gene45590:CDS
TTCCCCACAGAGTTTAGCAA+TGG	0.564444	3.4:+29894051	None:intergenic
GACACCATTGCTAAACTCTG+TGG	0.614594	3.4:-29894055	MS.gene45590:CDS
CACCATTGCTAAACTCTGTG+GGG	0.668610	3.4:-29894053	MS.gene45590:CDS
GACTTGTACCTTAGGAACAG+TGG	0.679559	3.4:+29893298	None:intergenic
ACAGTGGCAGATGAAAACTG+AGG	0.800494	3.4:+29893314	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAAATTAGAGTAAAATAT+AGG	+	chr3.4:29894045-29894064	None:intergenic	10.0%
!!	AAATTAGAGTAAAATATAGG+AGG	+	chr3.4:29894042-29894061	None:intergenic	20.0%
!!	AACCAAAAAAAGAAATTAAG+GGG	+	chr3.4:29893858-29893877	None:intergenic	20.0%
!!	AATCAAATATTTACTTGAAG+AGG	-	chr3.4:29893694-29893713	MS.gene45590:intron	20.0%
!!	ATCAAATATTTACTTGAAGA+GGG	-	chr3.4:29893695-29893714	MS.gene45590:intron	20.0%
!!	CAACCAAAAAAAGAAATTAA+GGG	+	chr3.4:29893859-29893878	None:intergenic	20.0%
!!	TCAACCAAAAAAAGAAATTA+AGG	+	chr3.4:29893860-29893879	None:intergenic	20.0%
!!	TCTTCAAGTAAATATTTGAT+TGG	+	chr3.4:29893695-29893714	None:intergenic	20.0%
!	ATAATCATTGAATGACTAAG+TGG	-	chr3.4:29893650-29893669	MS.gene45590:intron	25.0%
!	ATCATACCTTAAAAAAGTGA+CGG	+	chr3.4:29893342-29893361	None:intergenic	25.0%
!!!	AAAAGCATTAGGTTTACTTA+CGG	-	chr3.4:29893923-29893942	MS.gene45590:intron	25.0%
!!!	AACCCCTTAATTTCTTTTTT+TGG	-	chr3.4:29893853-29893872	MS.gene45590:intron	25.0%
!!!	AATATCCATCTAAAAGCATT+AGG	-	chr3.4:29893912-29893931	MS.gene45590:intron	25.0%
	AAAGAAATTAAGGGGTTGTT+TGG	+	chr3.4:29893850-29893869	None:intergenic	30.0%
	AATATTTACTTGAAGAGGGA+AGG	-	chr3.4:29893699-29893718	MS.gene45590:intron	30.0%
	ATATTTACTTGAAGAGGGAA+GGG	-	chr3.4:29893700-29893719	MS.gene45590:intron	30.0%
	ATTAGGTTTACTTACGGAAT+TGG	-	chr3.4:29893929-29893948	MS.gene45590:intron	30.0%
	CTCTAATTTAATTGTTTGCC+AGG	-	chr3.4:29894053-29894072	MS.gene45590:CDS	30.0%
	GATTGGATAGACATAATTGT+AGG	+	chr3.4:29893678-29893697	None:intergenic	30.0%
	GTTAATCTGTTGTGAAGAAA+TGG	-	chr3.4:29893405-29893424	MS.gene45590:CDS	30.0%
	TTAGGTTTACTTACGGAATT+GGG	-	chr3.4:29893930-29893949	MS.gene45590:intron	30.0%
	TTATAACCAACTCAAAGAGT+TGG	+	chr3.4:29893569-29893588	None:intergenic	30.0%
!	GTAAACCTAATGCTTTTAGA+TGG	+	chr3.4:29893920-29893939	None:intergenic	30.0%
!!	AACTCTTTGAGTTGGTTATA+AGG	-	chr3.4:29893568-29893587	MS.gene45590:intron	30.0%
!!!	TTTCTTCCGTCACTTTTTTA+AGG	-	chr3.4:29893333-29893352	MS.gene45590:CDS	30.0%
	ACCATTAAATGTGCAGAGAA+TGG	-	chr3.4:29893879-29893898	MS.gene45590:intron	35.0%
	ATGAACCGAATCTCTAAGTT+GGG	-	chr3.4:29893768-29893787	MS.gene45590:intron	35.0%
	TATGAACCGAATCTCTAAGT+TGG	-	chr3.4:29893767-29893786	MS.gene45590:intron	35.0%
	TCCATTCTCTGCACATTTAA+TGG	+	chr3.4:29893883-29893902	None:intergenic	35.0%
	TGTTCTTGAGCAATACGATT+TGG	-	chr3.4:29893439-29893458	MS.gene45590:intron	35.0%
	TTAAATGTGCAGAGAATGGA+AGG	-	chr3.4:29893883-29893902	MS.gene45590:intron	35.0%
!	AGAGTTGGTTAGGATTGATT+AGG	+	chr3.4:29893554-29893573	None:intergenic	35.0%
	ACACCATTGCTAAACTCTGT+GGG	-	chr3.4:29893261-29893280	MS.gene45590:intron	40.0%
	AGGCAGTGCTAATCTATGTA+AGG	-	chr3.4:29893588-29893607	MS.gene45590:intron	40.0%
	ATGTATCAGCTATGATGCTC+TGG	-	chr3.4:29893479-29893498	MS.gene45590:intron	40.0%
	CGAAACCCAACTTAGAGATT+CGG	+	chr3.4:29893776-29893795	None:intergenic	40.0%
	TATAGGAGGACTTGTACCTT+AGG	+	chr3.4:29894028-29894047	None:intergenic	40.0%
	TCCTAACCAACTCTTTGAGT+TGG	-	chr3.4:29893560-29893579	MS.gene45590:intron	40.0%
!	ACCAACTCAAAGAGTTGGTT+AGG	+	chr3.4:29893564-29893583	None:intergenic	40.0%
	ACAGTGGCAGATGAAAACTG+AGG	+	chr3.4:29894004-29894023	None:intergenic	45.0%
	CACCATTGCTAAACTCTGTG+GGG	-	chr3.4:29893262-29893281	MS.gene45590:intron	45.0%
	GACACCATTGCTAAACTCTG+TGG	-	chr3.4:29893260-29893279	MS.gene45590:intron	45.0%
	GACTTGTACCTTAGGAACAG+TGG	+	chr3.4:29894020-29894039	None:intergenic	45.0%
	GGCGATAGTGATACAAACTG+AGG	+	chr3.4:29893533-29893552	None:intergenic	45.0%
	TTCATCTGCCACTGTTCCTA+AGG	-	chr3.4:29894009-29894028	MS.gene45590:CDS	45.0%
	TTCCCCACAGAGTTTAGCAA+TGG	+	chr3.4:29893267-29893286	None:intergenic	45.0%
	TTGAAGAGGGAAGGGAAAGT+TGG	-	chr3.4:29893708-29893727	MS.gene45590:intron	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	29893257	29894080	29893257	ID=MS.gene45590
chr3.4	mRNA	29893257	29894080	29893257	ID=MS.gene45590.t1;Parent=MS.gene45590
chr3.4	exon	29893983	29894080	29893983	ID=MS.gene45590.t1.exon1;Parent=MS.gene45590.t1
chr3.4	CDS	29893983	29894080	29893983	ID=cds.MS.gene45590.t1;Parent=MS.gene45590.t1
chr3.4	exon	29893307	29893442	29893307	ID=MS.gene45590.t1.exon2;Parent=MS.gene45590.t1
chr3.4	CDS	29893307	29893442	29893307	ID=cds.MS.gene45590.t1;Parent=MS.gene45590.t1
chr3.4	exon	29893257	29893262	29893257	ID=MS.gene45590.t1.exon3;Parent=MS.gene45590.t1
chr3.4	CDS	29893257	29893262	29893257	ID=cds.MS.gene45590.t1;Parent=MS.gene45590.t1

Gene Sequence

>MS.gene45590

ATGGACACCATTGCTAAACTCTGTGGGGAAGAAGAACGCAACACTGCTGTTGAGCAAAGCGAGATCAAGAGAATGTTTTCTTCCGTCACTTTTTTAAGGTATGATTGCAAAGAACTAATTGTTTCATGAGTTTTTTGAATTTGTTGCTGTTAATCTGTTGTGAAGAAATGGCAAATGTGTTTTGTTCTTGAGCAATACGATTTGGTTGTATTATCGTTGAATATGTATCAGCTATGATGCTCTGGATTGACAACTATGATATATATCATGTAACCTCAGTTTGTATCACTATCGCCTAATCAATCCTAACCAACTCTTTGAGTTGGTTATAAGGCAGTGCTAATCTATGTAAGGTCTACTTTATTCTACAGTTGAGTTACATGAGTGTCTCTAATAATCATTGAATGACTAAGTGGTTCCTACAATTATGTCTATCCAATCAAATATTTACTTGAAGAGGGAAGGGAAAGTTGGATTAAAATAAAATCAACTTTGAAATTGTTTCTTTTTTATGAACCGAATCTCTAAGTTGGGTTTCGTGAGATTAAAACATATCTTCAATATATTGACTATATAGTTTTTAGAAGTTTCCAAACAACCCCTTAATTTCTTTTTTTGGTTGACCATTAAATGTGCAGAGAATGGAAGGATTTTAAATATCCATCTAAAAGCATTAGGTTTACTTACGGAATTGGGAGCTCTGAGTGTTATGAGAGAAATGATATCAAAGATGACATAAACTTACCTCAGTTTTCATCTGCCACTGTTCCTAAGGTACAAGTCCTCCTATATTTTACTCTAATTTAATTGTTTGCCAGGCATGA

Protein sequence

>MS.gene45590.t1

MDTIAKLCGEEERNTAVEQSEIKRMFSSVTFLREWKDFKYPSKSIRFTYGIGSSECYERNDIKDDINLPQFSSATVPKA