Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45737.t1 | Q9FVL0.1 | 99.4 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 7.20E-82 | 313.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45737.t1 | Q9FVL0 | 99.4 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 1.7e-84 | 313.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45737.t1 | I3SPW2 | 98.8 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 1.5e-81 | 311.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050380 | MS.gene45737 | 0.800529 | 1.38E-48 | -1.69E-46 |
| MS.gene050747 | MS.gene45737 | -0.802585 | 5.23E-49 | -1.69E-46 |
| MS.gene050926 | MS.gene45737 | 0.824915 | 6.23E-54 | -1.69E-46 |
| MS.gene053436 | MS.gene45737 | -0.802945 | 4.41E-49 | -1.69E-46 |
| MS.gene053873 | MS.gene45737 | -0.825473 | 4.60E-54 | -1.69E-46 |
| MS.gene054435 | MS.gene45737 | 0.823522 | 1.32E-53 | -1.69E-46 |
| MS.gene054437 | MS.gene45737 | 0.815181 | 1.05E-51 | -1.69E-46 |
| MS.gene054447 | MS.gene45737 | 0.814781 | 1.29E-51 | -1.69E-46 |
| MS.gene056514 | MS.gene45737 | -0.8048 | 1.82E-49 | -1.69E-46 |
| MS.gene056647 | MS.gene45737 | 0.824781 | 6.70E-54 | -1.69E-46 |
| MS.gene057506 | MS.gene45737 | 0.805993 | 1.02E-49 | -1.69E-46 |
| MS.gene059046 | MS.gene45737 | 0.8143 | 1.65E-51 | -1.69E-46 |
| MS.gene059944 | MS.gene45737 | 0.802702 | 4.95E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45737.t1 | MTR_4g068860 | 98.750 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 2.06e-114 | 321 |
| MS.gene45737.t1 | MTR_4g068870 | 82.550 | 149 | 26 | 0 | 9 | 157 | 49 | 197 | 4.00e-86 | 251 |
| MS.gene45737.t1 | MTR_4g068870 | 82.550 | 149 | 26 | 0 | 9 | 157 | 132 | 280 | 5.75e-85 | 251 |
| MS.gene45737.t1 | MTR_4g068870 | 83.486 | 109 | 18 | 0 | 49 | 157 | 1 | 109 | 7.41e-60 | 187 |
| MS.gene45737.t1 | MTR_4g068870 | 79.487 | 156 | 32 | 0 | 2 | 157 | 171 | 326 | 1.70e-84 | 252 |
| MS.gene45737.t1 | MTR_4g068870 | 78.846 | 156 | 30 | 1 | 2 | 157 | 10 | 162 | 1.17e-82 | 247 |
| MS.gene45737.t1 | MTR_4g068870 | 82.550 | 149 | 26 | 0 | 9 | 157 | 185 | 333 | 3.02e-84 | 251 |
| MS.gene45737.t1 | MTR_4g068870 | 78.846 | 156 | 30 | 1 | 2 | 157 | 10 | 162 | 1.26e-82 | 247 |
| MS.gene45737.t1 | MTR_0026s0210 | 80.667 | 150 | 29 | 0 | 8 | 157 | 13 | 162 | 5.40e-84 | 251 |
| MS.gene45737.t1 | MTR_0026s0210 | 81.208 | 149 | 28 | 0 | 9 | 157 | 185 | 333 | 1.83e-83 | 249 |
| MS.gene45737.t1 | MTR_4g068860 | 95.868 | 121 | 5 | 0 | 1 | 121 | 1 | 121 | 2.81e-81 | 236 |
| MS.gene45737.t1 | MTR_1g090810 | 48.000 | 150 | 77 | 1 | 8 | 156 | 3 | 152 | 7.91e-39 | 129 |
| MS.gene45737.t1 | MTR_5g041610 | 47.651 | 149 | 74 | 2 | 8 | 156 | 2 | 146 | 2.60e-36 | 122 |
| MS.gene45737.t1 | MTR_5g081030 | 45.638 | 149 | 77 | 2 | 8 | 156 | 2 | 146 | 5.69e-36 | 122 |
| MS.gene45737.t1 | MTR_5g080440 | 45.638 | 149 | 77 | 2 | 8 | 156 | 2 | 146 | 1.13e-35 | 121 |
| MS.gene45737.t1 | MTR_1g011540 | 47.651 | 149 | 73 | 3 | 8 | 156 | 2 | 145 | 9.97e-35 | 119 |
| MS.gene45737.t1 | MTR_5g081000 | 46.309 | 149 | 75 | 3 | 8 | 156 | 2 | 145 | 1.17e-33 | 116 |
| MS.gene45737.t1 | MTR_5g066070 | 45.270 | 148 | 77 | 2 | 9 | 156 | 3 | 146 | 2.32e-33 | 115 |
| MS.gene45737.t1 | MTR_5g080400 | 44.000 | 150 | 80 | 2 | 8 | 157 | 2 | 147 | 1.40e-32 | 113 |
| MS.gene45737.t1 | MTR_1g090820 | 44.295 | 149 | 80 | 1 | 8 | 156 | 2 | 147 | 2.39e-32 | 112 |
| MS.gene45737.t1 | MTR_1g049330 | 41.892 | 148 | 83 | 1 | 9 | 156 | 3 | 147 | 6.82e-31 | 109 |
| MS.gene45737.t1 | MTR_5g080900 | 39.189 | 148 | 71 | 4 | 9 | 156 | 3 | 131 | 1.73e-22 | 87.4 |
| MS.gene45737.t1 | MTR_1g090820 | 46.296 | 108 | 55 | 1 | 49 | 156 | 1 | 105 | 3.27e-22 | 85.9 |
| MS.gene45737.t1 | MTR_5g080400 | 44.954 | 109 | 56 | 2 | 49 | 157 | 1 | 105 | 6.35e-22 | 85.1 |
| MS.gene45737.t1 | MTR_5g066070 | 43.519 | 108 | 57 | 2 | 9 | 116 | 3 | 106 | 2.62e-20 | 80.9 |
| MS.gene45737.t1 | MTR_1g090820 | 41.284 | 109 | 61 | 1 | 8 | 116 | 2 | 107 | 4.30e-19 | 77.8 |
| MS.gene45737.t1 | MTR_7g110180 | 40.833 | 120 | 54 | 5 | 9 | 128 | 3 | 105 | 4.29e-15 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45737.t1 | AT2G16060 | 78.947 | 152 | 32 | 0 | 9 | 160 | 9 | 160 | 1.95e-87 | 253 |
| MS.gene45737.t1 | AT3G10520 | 52.667 | 150 | 71 | 0 | 8 | 157 | 5 | 154 | 2.34e-50 | 159 |
Find 26 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGACAAAGTTTGCACTTT+TGG | 0.206749 | 4.4:+38618865 | MS.gene45737:intron |
| CTACTACACCATATTTAAAA+TGG | 0.249717 | 4.4:-38618671 | None:intergenic |
| AAAAGATTCAAAAGTTCCTT+TGG | 0.334733 | 4.4:+38618076 | MS.gene45737:CDS |
| TTCTCTGACCGTGACTTTAC+CGG | 0.339739 | 4.4:-38618622 | None:intergenic |
| CCTTCAGAATATGGGCACTT+TGG | 0.384904 | 4.4:+38617808 | None:intergenic |
| TATAAACTAGAGAGTTTAAG+AGG | 0.389161 | 4.4:-38618996 | None:intergenic |
| AAGAAGAATTCTGCAGAGTT+AGG | 0.397154 | 4.4:+38617890 | MS.gene45737:CDS |
| AACTAGAGAGTTTAAGAGGA+AGG | 0.427518 | 4.4:-38618992 | None:intergenic |
| ATAGCCGGTGACCACATTTC+AGG | 0.433187 | 4.4:-38618908 | None:intergenic |
| TGCCATGTTTGTCTTTCTCA+TGG | 0.438184 | 4.4:+38618124 | MS.gene45737:CDS |
| GCAGTTCAATTGCGGAAATC+CGG | 0.465498 | 4.4:+38618603 | MS.gene45737:CDS |
| ATGGGCACTTTGGATACAAA+AGG | 0.471419 | 4.4:+38617818 | MS.gene45737:CDS |
| CCCCATGCATTCTTCATAGC+CGG | 0.478259 | 4.4:-38618923 | None:intergenic |
| CACCGGCTATGAAGAATGCA+TGG | 0.521161 | 4.4:+38618921 | MS.gene45737:CDS |
| AAGCTCTTGTGGTGAAGTCA+TGG | 0.525782 | 4.4:+38617858 | MS.gene45737:CDS |
| GGGAGAAGCTTATGATCAGT+TGG | 0.552837 | 4.4:+38618943 | MS.gene45737:CDS |
| ACCGGCTATGAAGAATGCAT+GGG | 0.592597 | 4.4:+38618922 | MS.gene45737:CDS |
| AGTACCTGAAATGTGGTCAC+CGG | 0.611768 | 4.4:+38618904 | MS.gene45737:CDS |
| GTGAATCAGCAGTTCAATTG+CGG | 0.612574 | 4.4:+38618595 | MS.gene45737:CDS |
| TGAAGAACAAGAAGCTCTTG+TGG | 0.616139 | 4.4:+38617847 | MS.gene45737:CDS |
| CATGGCATGAGGCTTGAGCT+TGG | 0.623542 | 4.4:-38618108 | None:intergenic |
| AAGAAGCAGTACCTGAAATG+TGG | 0.626355 | 4.4:+38618897 | MS.gene45737:CDS |
| GCGGAAATCCGGTAAAGTCA+CGG | 0.634999 | 4.4:+38618614 | MS.gene45737:CDS |
| AGAAAGACAAACATGGCATG+AGG | 0.657273 | 4.4:-38618119 | None:intergenic |
| TACCATGAGAAAGACAAACA+TGG | 0.700973 | 4.4:-38618126 | None:intergenic |
| CCGGCTATGAAGAATGCATG+GGG | 0.704265 | 4.4:+38618923 | MS.gene45737:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAGTATATTTTTTTTACT+AGG | + | chr4.4:38618724-38618743 | MS.gene45737:intron | 10.0% |
| !!! | TAAGTATATTTTTTTTACTA+GGG | + | chr4.4:38618725-38618744 | MS.gene45737:intron | 10.0% |
| !!! | TTTAATTTTTTGATTTTGTA+AGG | + | chr4.4:38618844-38618863 | MS.gene45737:intron | 10.0% |
| !! | TTACATTAAAATTTGACATA+AGG | + | chr4.4:38618780-38618799 | MS.gene45737:intron | 15.0% |
| !! | TATCATTTCTCATATTCAAA+TGG | - | chr4.4:38618349-38618368 | None:intergenic | 20.0% |
| !! | TTAAAATTTGACATAAGGTT+TGG | + | chr4.4:38618785-38618804 | MS.gene45737:intron | 20.0% |
| !!! | CTAATTCAGTAACTAAATTT+TGG | + | chr4.4:38618211-38618230 | MS.gene45737:intron | 20.0% |
| ! | AAAAGATTCAAAAGTTCCTT+TGG | + | chr4.4:38618076-38618095 | MS.gene45737:CDS | 25.0% |
| ! | AATAAGAAGTGAGAAAAACA+AGG | + | chr4.4:38618473-38618492 | MS.gene45737:intron | 25.0% |
| ! | CTACTACACCATATTTAAAA+TGG | - | chr4.4:38618674-38618693 | None:intergenic | 25.0% |
| ! | GAATCAAGCTTGAAAAAATT+AGG | + | chr4.4:38618642-38618661 | MS.gene45737:CDS | 25.0% |
| ! | GCAATAACAAAATAATTAGC+TGG | - | chr4.4:38618569-38618588 | None:intergenic | 25.0% |
| ! | TCAACAAAGTTATCATCAAA+TGG | + | chr4.4:38618499-38618518 | MS.gene45737:intron | 25.0% |
| ! | TTCTTATTGGTCAAAAACAA+TGG | - | chr4.4:38618461-38618480 | None:intergenic | 25.0% |
| ! | TTTAGTTACTGAATTAGAGA+CGG | - | chr4.4:38618208-38618227 | None:intergenic | 25.0% |
| !!! | TTCAACATTTTTTACTCAAG+AGG | + | chr4.4:38617950-38617969 | MS.gene45737:intron | 25.0% |
| !!! | TTGTTTTTCTCACTTCTTAT+TGG | - | chr4.4:38618474-38618493 | None:intergenic | 25.0% |
| !!! | TTTTTTTGAAGTAAGTAGCT+AGG | + | chr4.4:38617921-38617940 | MS.gene45737:intron | 25.0% |
| AAAACAATGGAGAGAGAAAA+AGG | - | chr4.4:38618448-38618467 | None:intergenic | 30.0% | |
| ! | GGTGCTAACCATTTTAAATA+TGG | + | chr4.4:38618663-38618682 | MS.gene45737:CDS | 30.0% |
| !!! | AACCACTTTTTGACAACTTT+TGG | + | chr4.4:38618376-38618395 | MS.gene45737:intron | 30.0% |
| AAATCCGTCTCTAATGTACT+CGG | - | chr4.4:38618238-38618257 | None:intergenic | 35.0% | |
| AAGAAGAATTCTGCAGAGTT+AGG | + | chr4.4:38617890-38617909 | MS.gene45737:CDS | 35.0% | |
| GTCCAAAAGTTGTCAAAAAG+TGG | - | chr4.4:38618381-38618400 | None:intergenic | 35.0% | |
| TACCATGAGAAAGACAAACA+TGG | - | chr4.4:38618129-38618148 | None:intergenic | 35.0% | |
| TCTCATATTCAAATGGTGAG+TGG | - | chr4.4:38618342-38618361 | None:intergenic | 35.0% | |
| TGACAAAGTCAAAGCTTGAA+TGG | - | chr4.4:38618312-38618331 | None:intergenic | 35.0% | |
| !! | TTCAGGTACTGCTTCTTTTA+TGG | - | chr4.4:38618894-38618913 | None:intergenic | 35.0% |
| AAGAAGCAGTACCTGAAATG+TGG | + | chr4.4:38618897-38618916 | MS.gene45737:CDS | 40.0% | |
| AGAAAGACAAACATGGCATG+AGG | - | chr4.4:38618122-38618141 | None:intergenic | 40.0% | |
| AGAGAGAAAAAGGAAGAGAG+AGG | - | chr4.4:38618438-38618457 | None:intergenic | 40.0% | |
| ATGGGCACTTTGGATACAAA+AGG | + | chr4.4:38617818-38617837 | MS.gene45737:CDS | 40.0% | |
| GTGAATCAGCAGTTCAATTG+CGG | + | chr4.4:38618595-38618614 | MS.gene45737:CDS | 40.0% | |
| TGAAGAACAAGAAGCTCTTG+TGG | + | chr4.4:38617847-38617866 | MS.gene45737:CDS | 40.0% | |
| TGCCATGTTTGTCTTTCTCA+TGG | + | chr4.4:38618124-38618143 | MS.gene45737:CDS | 40.0% | |
| ! | GGTGACAAAGTTTGCACTTT+TGG | + | chr4.4:38618865-38618884 | MS.gene45737:intron | 40.0% |
| ! | TGTAGTAGACGAGCATTTTG+AGG | + | chr4.4:38618686-38618705 | MS.gene45737:CDS | 40.0% |
| !! | GAGAACAGTTTTTGAGCTGA+TGG | - | chr4.4:38618050-38618069 | None:intergenic | 40.0% |
| AAGCTCTTGTGGTGAAGTCA+TGG | + | chr4.4:38617858-38617877 | MS.gene45737:CDS | 45.0% | |
| ACCGGCTATGAAGAATGCAT+GGG | + | chr4.4:38618922-38618941 | MS.gene45737:CDS | 45.0% | |
| AGTACCTGAAATGTGGTCAC+CGG | + | chr4.4:38618904-38618923 | MS.gene45737:CDS | 45.0% | |
| AGTCAAAGCTTGAATGGTCG+TGG | - | chr4.4:38618306-38618325 | None:intergenic | 45.0% | |
| GCAGTTCAATTGCGGAAATC+CGG | + | chr4.4:38618603-38618622 | MS.gene45737:CDS | 45.0% | |
| GGGAGAAGCTTATGATCAGT+TGG | + | chr4.4:38618943-38618962 | MS.gene45737:CDS | 45.0% | |
| TGGACCGAGTACATTAGAGA+CGG | + | chr4.4:38618231-38618250 | MS.gene45737:intron | 45.0% | |
| TTCTCTGACCGTGACTTTAC+CGG | - | chr4.4:38618625-38618644 | None:intergenic | 45.0% | |
| !!! | AGCTTGGTGTTTTGCTCCAA+AGG | - | chr4.4:38618095-38618114 | None:intergenic | 45.0% |
| ATAGCCGGTGACCACATTTC+AGG | - | chr4.4:38618911-38618930 | None:intergenic | 50.0% | |
| CCCCATGCATTCTTCATAGC+CGG | - | chr4.4:38618926-38618945 | None:intergenic | 50.0% | |
| CCGGCTATGAAGAATGCATG+GGG | + | chr4.4:38618923-38618942 | MS.gene45737:CDS | 50.0% | |
| GCGGAAATCCGGTAAAGTCA+CGG | + | chr4.4:38618614-38618633 | MS.gene45737:CDS | 50.0% | |
| ! | CACCGGCTATGAAGAATGCA+TGG | + | chr4.4:38618921-38618940 | MS.gene45737:CDS | 50.0% |
| CATGGCATGAGGCTTGAGCT+TGG | - | chr4.4:38618111-38618130 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 38617818 | 38619003 | 38617818 | ID=MS.gene45737 |
| chr4.4 | mRNA | 38617818 | 38619003 | 38617818 | ID=MS.gene45737.t1;Parent=MS.gene45737 |
| chr4.4 | exon | 38617818 | 38617930 | 38617818 | ID=MS.gene45737.t1.exon1;Parent=MS.gene45737.t1 |
| chr4.4 | CDS | 38617818 | 38617930 | 38617818 | ID=cds.MS.gene45737.t1;Parent=MS.gene45737.t1 |
| chr4.4 | exon | 38618031 | 38618145 | 38618031 | ID=MS.gene45737.t1.exon2;Parent=MS.gene45737.t1 |
| chr4.4 | CDS | 38618031 | 38618145 | 38618031 | ID=cds.MS.gene45737.t1;Parent=MS.gene45737.t1 |
| chr4.4 | exon | 38618591 | 38618707 | 38618591 | ID=MS.gene45737.t1.exon3;Parent=MS.gene45737.t1 |
| chr4.4 | CDS | 38618591 | 38618707 | 38618591 | ID=cds.MS.gene45737.t1;Parent=MS.gene45737.t1 |
| chr4.4 | exon | 38618866 | 38619003 | 38618866 | ID=MS.gene45737.t1.exon4;Parent=MS.gene45737.t1 |
| chr4.4 | CDS | 38618866 | 38619003 | 38618866 | ID=cds.MS.gene45737.t1;Parent=MS.gene45737.t1 |
| Gene Sequence |
| Protein sequence |
