AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46686.t1 MTR_3g030540 92.906 437 31 0 4 440 1 437 0.0 828
MS.gene46686.t1 MTR_2g060350 62.247 445 158 6 4 440 1 443 0.0 560
MS.gene46686.t1 MTR_1g052315 50.346 433 201 3 16 438 2 430 5.55e-146 424
MS.gene46686.t1 MTR_7g091850 50.457 438 208 4 9 438 7 443 1.45e-140 410
MS.gene46686.t1 MTR_7g091840 48.942 378 183 5 9 382 7 378 3.86e-119 353
MS.gene46686.t1 MTR_2g103410 46.594 367 193 2 73 436 59 425 1.19e-110 333
MS.gene46686.t1 MTR_8g065030 44.293 368 203 2 49 414 41 408 1.19e-107 326
MS.gene46686.t1 MTR_6g005630 44.886 352 185 6 69 418 41 385 4.23e-97 298
MS.gene46686.t1 MTR_2g049400 41.476 393 217 9 48 437 6 388 5.91e-87 271
MS.gene46686.t1 MTR_4g084050 40.104 384 204 8 63 431 13 385 5.05e-85 266
MS.gene46686.t1 MTR_7g110050 40.111 359 195 6 74 417 68 421 9.78e-85 267
MS.gene46686.t1 MTR_1g086390 40.214 373 206 8 73 431 81 450 3.05e-83 264
MS.gene46686.t1 MTR_3g438050 42.363 347 188 8 71 411 25 365 7.01e-83 260
MS.gene46686.t1 MTR_3g084380 40.489 368 200 7 54 412 2 359 9.44e-83 260
MS.gene46686.t1 MTR_6g005630 42.045 352 172 8 69 418 41 362 1.55e-82 259
MS.gene46686.t1 MTR_8g065010 38.220 382 198 7 65 411 94 472 3.15e-82 262
MS.gene46686.t1 MTR_3g054320 40.997 361 190 9 74 425 31 377 4.91e-82 258
MS.gene46686.t1 MTR_3g084360 39.247 372 211 6 67 431 18 381 8.04e-82 257
MS.gene46686.t1 MTR_4g083660 42.175 377 206 10 66 437 2 371 8.60e-82 257
MS.gene46686.t1 MTR_6g028080 40.379 369 194 8 72 427 29 384 1.77e-81 257
MS.gene46686.t1 MTR_4g094715 41.076 353 187 7 74 411 66 412 1.42e-80 256
MS.gene46686.t1 MTR_5g033680 37.604 359 213 6 61 411 47 402 4.85e-80 255
MS.gene46686.t1 MTR_8g009810 41.600 375 208 9 70 439 32 400 1.28e-79 252
MS.gene46686.t1 MTR_5g034090 37.824 386 204 9 59 411 103 485 1.69e-78 253
MS.gene46686.t1 MTR_5g043350 39.130 368 210 5 66 426 22 382 2.10e-78 249
MS.gene46686.t1 MTR_7g113910 48.062 258 116 1 166 423 95 334 4.87e-78 247
MS.gene46686.t1 MTR_2g435640 38.172 372 215 11 70 432 50 415 6.30e-78 248
MS.gene46686.t1 MTR_2g103650 40.580 345 197 5 72 409 27 370 1.99e-77 246
MS.gene46686.t1 MTR_8g010080 39.888 356 194 7 74 425 32 371 6.02e-77 244
MS.gene46686.t1 MTR_1g088160 39.601 351 195 11 74 415 36 378 2.23e-76 244
MS.gene46686.t1 MTR_8g030930 40.811 370 198 10 55 415 15 372 3.69e-76 243
MS.gene46686.t1 MTR_4g120320 39.370 381 214 12 70 440 49 422 7.79e-74 238
MS.gene46686.t1 MTR_1g012390 38.798 366 207 9 69 426 24 380 3.25e-73 235
MS.gene46686.t1 MTR_2g103410 46.718 259 135 2 73 328 59 317 4.29e-72 230
MS.gene46686.t1 MTR_8g010090 38.375 357 199 8 74 425 32 372 2.34e-71 230
MS.gene46686.t1 MTR_2g058840 37.604 359 214 7 73 426 25 378 9.94e-71 229
MS.gene46686.t1 MTR_8g010110 38.375 357 199 8 74 425 32 372 9.98e-71 229
MS.gene46686.t1 MTR_2g032710 38.292 363 211 6 60 411 54 414 3.19e-70 229
MS.gene46686.t1 MTR_8g010040 38.874 373 206 10 70 437 29 384 4.10e-70 227
MS.gene46686.t1 MTR_2g058670 36.828 372 201 8 73 426 25 380 1.38e-69 226
MS.gene46686.t1 MTR_0157s0060 40.169 356 194 5 77 425 2 345 2.03e-69 224
MS.gene46686.t1 MTR_4g034020 40.169 356 194 5 77 425 2 345 2.03e-69 224
MS.gene46686.t1 MTR_1g013740 36.631 374 210 9 70 437 32 384 6.44e-69 224
MS.gene46686.t1 MTR_8g010070 38.997 359 195 13 74 425 32 373 1.42e-67 220
MS.gene46686.t1 MTR_8g008765 38.906 329 182 6 74 398 32 345 5.75e-67 218
MS.gene46686.t1 MTR_2g058640 37.640 356 209 8 91 438 41 391 1.06e-66 219
MS.gene46686.t1 MTR_2g029170 38.485 330 194 8 111 437 35 358 3.12e-66 216
MS.gene46686.t1 MTR_1g013730 35.657 373 213 9 70 436 24 375 1.30e-65 215
MS.gene46686.t1 MTR_7g035415 41.356 295 153 8 71 357 38 320 2.71e-65 213
MS.gene46686.t1 MTR_5g063430 37.459 307 178 6 63 364 15 312 2.00e-59 197
MS.gene46686.t1 MTR_5g034090 36.184 304 158 6 59 328 103 404 9.59e-58 196
MS.gene46686.t1 MTR_8g008700 41.538 260 135 4 150 405 31 277 1.89e-57 191
MS.gene46686.t1 MTR_8g077320 39.735 302 137 7 71 357 31 302 2.36e-57 193
MS.gene46686.t1 MTR_5g097860 34.056 323 202 7 71 387 23 340 6.72e-56 189
MS.gene46686.t1 MTR_8g010050 33.520 358 182 11 74 425 32 339 2.45e-50 174
MS.gene46686.t1 MTR_8g031180 44.231 208 108 2 211 413 70 274 2.54e-48 167
MS.gene46686.t1 MTR_2g100040 31.356 354 185 11 63 409 14 316 7.88e-42 152
MS.gene46686.t1 MTR_2g042740 28.528 326 198 11 138 437 128 444 2.39e-26 111
MS.gene46686.t1 MTR_4g124980 25.532 423 259 17 54 436 55 461 1.31e-23 103
MS.gene46686.t1 MTR_3g449590 25.000 396 257 12 63 426 37 424 2.24e-22 99.4
MS.gene46686.t1 MTR_1g086030 29.825 285 167 7 125 382 37 315 1.31e-21 96.7
MS.gene46686.t1 MTR_7g110710 29.573 328 191 14 140 440 117 431 4.94e-21 95.5
MS.gene46686.t1 MTR_7g110710 29.573 328 191 14 140 440 117 431 7.42e-21 95.1
MS.gene46686.t1 MTR_4g120730 27.891 294 187 9 159 436 143 427 2.44e-20 93.6
MS.gene46686.t1 MTR_1g086030 31.481 270 152 8 140 382 156 419 8.73e-20 92.0
MS.gene46686.t1 MTR_7g078680 24.923 325 215 8 137 438 126 444 2.29e-19 90.5
MS.gene46686.t1 MTR_5g090160 25.130 386 238 12 63 411 34 405 2.52e-19 90.1
MS.gene46686.t1 MTR_3g096330 26.174 298 189 9 159 438 179 463 1.31e-18 89.0
MS.gene46686.t1 MTR_8g098840 25.279 269 177 8 158 411 146 405 1.56e-18 88.2
MS.gene46686.t1 MTR_8g028725 24.874 398 263 13 71 438 53 444 2.26e-18 87.4
MS.gene46686.t1 MTR_7g088550 27.703 296 178 11 141 411 128 412 4.67e-18 86.7
MS.gene46686.t1 MTR_1g100060 25.589 297 187 10 140 411 167 454 7.07e-18 86.3
MS.gene46686.t1 MTR_1g100050 26.022 269 175 8 158 411 152 411 1.89e-17 84.7
MS.gene46686.t1 MTR_2g435340 25.538 325 208 9 130 436 51 359 1.85e-16 81.3
MS.gene46686.t1 MTR_2g435340 25.397 315 202 8 130 427 116 414 3.15e-16 80.9
MS.gene46686.t1 MTR_1g099850 24.762 315 200 12 140 427 129 433 6.10e-16 80.1
MS.gene46686.t1 MTR_1g100040 25.436 287 183 9 140 404 133 410 1.78e-15 78.6
MS.gene46686.t1 MTR_8g007320 50.847 59 28 1 367 425 22 79 3.01e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46686.t1 AT3G07970 53.452 449 184 10 4 438 1 438 1.27e-154 445
MS.gene46686.t1 AT3G57510 46.759 432 212 7 13 438 11 430 1.34e-129 381
MS.gene46686.t1 AT2G41850 47.465 434 213 7 9 438 8 430 6.30e-121 359
MS.gene46686.t1 AT1G80170 45.758 389 205 4 53 436 33 420 6.81e-115 344
MS.gene46686.t1 AT1G23460 44.855 379 204 4 61 436 31 407 2.20e-107 325
MS.gene46686.t1 AT1G23460 44.855 379 204 4 61 436 55 431 3.18e-107 325
MS.gene46686.t1 AT1G70500 42.466 438 231 9 9 436 5 431 3.19e-106 323
MS.gene46686.t1 AT1G56710 42.553 376 184 9 61 411 29 397 2.37e-91 283
MS.gene46686.t1 AT5G14650 41.047 363 206 7 51 411 30 386 1.08e-88 276
MS.gene46686.t1 AT4G35670 42.582 364 194 7 74 431 25 379 6.15e-87 271
MS.gene46686.t1 AT2G43890 38.000 400 232 10 41 437 3 389 4.78e-86 268
MS.gene46686.t1 AT5G27530 42.618 359 183 6 74 426 54 395 4.67e-85 268
MS.gene46686.t1 AT5G44840 40.111 359 201 6 74 426 25 375 2.21e-84 264
MS.gene46686.t1 AT1G05660 39.683 378 217 9 63 436 18 388 5.95e-84 263
MS.gene46686.t1 AT3G15720 41.689 367 194 8 69 426 19 374 2.17e-83 263
MS.gene46686.t1 AT3G15720 41.689 367 194 8 69 426 20 375 2.40e-83 263
MS.gene46686.t1 AT4G18180 39.062 384 210 8 73 436 39 418 7.64e-83 261
MS.gene46686.t1 AT2G43870 41.755 376 204 13 69 438 17 383 8.89e-83 259
MS.gene46686.t1 AT1G05650 39.617 366 212 8 74 436 29 388 1.46e-82 259
MS.gene46686.t1 AT1G02460 41.783 359 181 9 74 411 90 441 5.38e-82 261
MS.gene46686.t1 AT2G43860 42.785 395 206 13 51 439 18 398 7.08e-82 258
MS.gene46686.t1 AT2G43880 40.000 375 215 9 66 437 24 391 1.24e-81 257
MS.gene46686.t1 AT3G15720 41.689 367 191 9 69 426 19 371 3.68e-81 258
MS.gene46686.t1 AT3G59850 41.779 371 202 12 72 436 22 384 4.49e-81 255
MS.gene46686.t1 AT3G59850 41.779 371 202 12 72 436 66 428 5.65e-81 256
MS.gene46686.t1 AT3G26610 35.748 428 246 10 1 415 1 412 2.37e-79 253
MS.gene46686.t1 AT1G80170 45.714 280 147 3 53 328 33 311 2.44e-79 249
MS.gene46686.t1 AT1G80140 40.882 340 184 8 108 439 6 336 3.16e-79 249
MS.gene46686.t1 AT1G48100 37.640 356 201 7 74 414 81 430 2.63e-77 248
MS.gene46686.t1 AT1G65570 38.235 374 220 9 68 436 26 393 2.76e-77 246
MS.gene46686.t1 AT4G01890 37.931 377 208 8 61 417 53 423 6.75e-77 247
MS.gene46686.t1 AT1G60590 36.314 369 202 6 74 411 124 490 1.00e-76 249
MS.gene46686.t1 AT1G10640 38.798 366 194 6 74 411 120 483 1.04e-76 248
MS.gene46686.t1 AT3G15720 43.260 319 165 6 114 426 3 311 1.14e-76 244
MS.gene46686.t1 AT4G32380 39.255 349 197 7 73 413 26 367 7.54e-76 244
MS.gene46686.t1 AT1G02790 34.325 437 237 12 17 438 19 420 7.58e-76 243
MS.gene46686.t1 AT3G07830 37.845 362 213 8 82 437 30 385 3.81e-75 240
MS.gene46686.t1 AT4G13760 38.035 397 224 11 52 437 15 400 3.90e-75 241
MS.gene46686.t1 AT5G48140 37.097 372 221 8 72 437 20 384 6.21e-75 240
MS.gene46686.t1 AT2G33160 38.060 402 231 10 44 436 7 399 7.55e-75 247
MS.gene46686.t1 AT4G35670 47.510 261 129 2 152 412 1 253 2.50e-74 234
MS.gene46686.t1 AT5G44830 39.692 325 177 4 104 426 9 316 3.35e-74 236
MS.gene46686.t1 AT5G17200 38.482 369 193 7 69 413 35 393 1.14e-73 237
MS.gene46686.t1 AT2G40310 37.531 397 226 11 52 437 15 400 2.75e-73 236
MS.gene46686.t1 AT3G07820 36.828 372 223 8 72 437 19 384 9.42e-73 234
MS.gene46686.t1 AT1G17150 38.211 369 217 8 74 436 34 397 5.18e-72 232
MS.gene46686.t1 AT1G43080 36.476 403 234 11 46 437 9 400 8.85e-72 232
MS.gene46686.t1 AT3G07840 36.193 373 224 8 72 437 20 385 1.14e-70 229
MS.gene46686.t1 AT1G43090 36.432 398 231 11 46 432 9 395 1.89e-70 229
MS.gene46686.t1 AT1G43100 36.683 398 230 11 46 432 9 395 5.24e-70 228
MS.gene46686.t1 AT2G15460 36.203 395 230 11 54 437 17 400 8.23e-70 227
MS.gene46686.t1 AT2G15450 36.020 397 231 12 54 438 17 402 5.86e-69 224
MS.gene46686.t1 AT3G07850 39.402 368 215 7 73 436 70 433 5.98e-68 223
MS.gene46686.t1 AT1G78400 36.639 363 219 8 70 426 32 389 6.85e-68 222
MS.gene46686.t1 AT3G14040 39.024 369 217 7 72 436 70 434 1.26e-67 222
MS.gene46686.t1 AT2G15470 35.696 395 232 11 54 437 17 400 3.53e-67 220
MS.gene46686.t1 AT2G26620 36.461 373 222 9 74 437 32 398 1.75e-65 215
MS.gene46686.t1 AT4G32375 40.794 277 155 3 149 423 9 278 1.31e-63 211
MS.gene46686.t1 AT4G32370 38.312 308 170 8 68 363 29 328 5.28e-63 207
MS.gene46686.t1 AT5G14650 39.286 280 164 5 51 328 30 305 1.40e-62 205
MS.gene46686.t1 AT2G43880 40.000 305 174 8 66 367 24 322 7.13e-62 204
MS.gene46686.t1 AT4G32380 35.447 347 172 7 73 413 26 326 4.81e-60 201
MS.gene46686.t1 AT5G39910 31.186 388 189 6 71 437 34 364 2.33e-53 183
MS.gene46686.t1 AT3G42950 27.960 397 242 13 74 436 67 453 4.97e-27 113
MS.gene46686.t1 AT3G62110 26.570 414 249 15 48 426 27 420 3.53e-26 110
MS.gene46686.t1 AT3G62110 26.570 414 249 15 48 426 27 420 3.53e-26 110
MS.gene46686.t1 AT4G23820 26.884 398 247 12 63 427 31 417 1.35e-24 105
MS.gene46686.t1 AT1G19170 24.464 466 291 17 12 437 32 476 4.46e-24 105
MS.gene46686.t1 AT3G48950 25.503 298 187 9 140 411 119 407 7.00e-19 89.0
MS.gene46686.t1 AT2G23900 25.651 269 177 7 158 411 158 418 8.62e-19 89.0
MS.gene46686.t1 AT4G33440 27.036 307 185 10 154 438 170 459 1.44e-18 88.2
MS.gene46686.t1 AT5G41870 24.806 387 242 13 63 413 35 408 4.63e-18 86.7
MS.gene46686.t1 AT5G49215 27.881 269 170 6 161 414 148 407 1.29e-17 85.1
MS.gene46686.t1 AT5G49215 27.881 269 170 6 161 414 146 405 1.33e-17 85.1
MS.gene46686.t1 AT3G16850 25.503 298 192 8 147 427 131 415 1.41e-16 82.0
MS.gene46686.t1 AT3G06770 26.837 313 196 8 147 440 47 345 1.56e-16 81.3
MS.gene46686.t1 AT4G23500 26.050 238 155 6 158 381 170 400 1.94e-16 81.6
MS.gene46686.t1 AT3G06770 26.837 313 196 8 147 440 65 363 2.65e-16 80.5
MS.gene46686.t1 AT3G06770 26.837 313 196 8 147 440 65 363 2.65e-16 80.5
MS.gene46686.t1 AT3G06770 26.837 313 196 8 147 440 134 432 4.84e-16 80.5
MS.gene46686.t1 AT3G06770 26.837 313 196 8 147 440 135 433 5.32e-16 80.1
MS.gene46686.t1 AT3G61490 24.062 320 220 7 131 433 131 444 5.51e-16 80.5
MS.gene46686.t1 AT3G61490 24.845 322 215 8 131 433 229 542 5.98e-16 80.5
MS.gene46686.t1 AT3G61490 24.062 320 220 7 131 433 116 429 9.03e-16 79.7
MS.gene46686.t1 AT3G61490 24.062 320 220 7 131 433 116 429 9.03e-16 79.7
MS.gene46686.t1 AT3G61490 24.062 320 220 7 131 433 116 429 9.03e-16 79.7

Find 86 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACCCAATACTGCATTAA+AGG 0.186184 3.3:+22988143 MS.gene46686:CDS
GCCTCATTTGGTTTGTGTTT+TGG 0.200442 3.3:+22987505 MS.gene46686:CDS
CAAACCAAATGAGGCTAAAA+TGG 0.240282 3.3:-22987497 None:intergenic
GTTTCATTATGTTAGATCTA+TGG 0.262928 3.3:+22988250 MS.gene46686:intron
ATCTAAATGCAGGGAGGTTC+TGG 0.263116 3.3:+22990764 MS.gene46686:intron
CATGGCCCTGAAAATGTTAT+AGG 0.267346 3.3:-22988120 None:intergenic
GCTCCATATGCTGAAATATT+AGG 0.274821 3.3:-22988289 None:intergenic
TTGAAGGATGCAACAATGTT+TGG 0.280649 3.3:+22989141 MS.gene46686:CDS
ACATGAACAATTTAGTTTCA+TGG 0.283475 3.3:+22987597 MS.gene46686:CDS
TTTACCTTTAATGCAGTATT+GGG 0.302256 3.3:-22988147 None:intergenic
GTGATGTACCAAAACATAAA+AGG 0.305023 3.3:+22991390 MS.gene46686:CDS
AACTTGTCCATCATTAGATT+TGG 0.311900 3.3:-22987692 None:intergenic
ATGCACAACAAATGCATATA+AGG 0.313150 3.3:+22989117 MS.gene46686:CDS
AAACTAAATTGTTCATGTAT+TGG 0.329621 3.3:-22987592 None:intergenic
GATGGTGGTGGTACCATTAA+TGG 0.331489 3.3:+22988361 MS.gene46686:CDS
AATATTAGGCCATGCTTTGA+TGG 0.334356 3.3:-22988275 None:intergenic
CTTTATTCTGACTCCATTTG+TGG 0.335167 3.3:-22990084 None:intergenic
GTGAACAGAGCCACACTAAT+AGG 0.355997 3.3:+22990059 MS.gene46686:CDS
ATTTACCTTTAATGCAGTAT+TGG 0.379250 3.3:-22988148 None:intergenic
ATAATTAACATTGTCACATT+TGG 0.385288 3.3:-22991526 None:intergenic
AGATGATAGACACTATGTTT+AGG 0.399830 3.3:-22988093 None:intergenic
TGCGGCGAAGGCGCGATAGA+TGG 0.401102 3.3:-22987634 None:intergenic
TGAAGGATGCAACAATGTTT+GGG 0.406265 3.3:+22989142 MS.gene46686:CDS
ACGGTTAACGTTGACGACTA+TGG 0.411063 3.3:+22987667 MS.gene46686:CDS
TGGCCTAATATTTCAGCATA+TGG 0.414557 3.3:+22988286 MS.gene46686:CDS
TTCACCAACCTCTCATTGTA+AGG 0.419899 3.3:-22987568 None:intergenic
AATGTTAATTATTCAAATAG+TGG 0.428526 3.3:+22991537 MS.gene46686:CDS
GTGGACCAGGACATGGAATC+AGG 0.439750 3.3:+22989848 MS.gene46686:CDS
TTATGAAAACATTCTCTGAA+TGG 0.443046 3.3:-22989226 None:intergenic
GGTGGTACCATTAATGGAAA+TGG 0.446333 3.3:+22988367 MS.gene46686:CDS
TATGAAAACATTCTCTGAAT+GGG 0.447211 3.3:-22989225 None:intergenic
TTTGTTCATGGGTAACACTT+AGG 0.447678 3.3:-22991570 None:intergenic
TTCATAACCAACAGCACCAT+TGG 0.449118 3.3:+22989242 MS.gene46686:CDS
TGCAAGTGAATTCCTTTGCA+TGG 0.456208 3.3:-22991462 None:intergenic
CAAATGCATATAAGGATTGA+AGG 0.456482 3.3:+22989125 MS.gene46686:CDS
ACTGTTGGTGCCTCCTTGCA+TGG 0.457070 3.3:-22988940 None:intergenic
CCTTTAATGCAGTATTGGGT+TGG 0.462412 3.3:-22988143 None:intergenic
TTGGCTCTTACTTAGAAGAA+AGG 0.465871 3.3:+22987524 MS.gene46686:CDS
TGGAATGAGGCATGTTCTAA+AGG 0.468725 3.3:+22988054 MS.gene46686:CDS
TTTAGGCATTTGAAATGTCA+TGG 0.469564 3.3:+22988034 MS.gene46686:intron
ACCTGTCCCAATGGTGCTGT+TGG 0.469575 3.3:-22989249 None:intergenic
AACAATTTAGTTTCATGGCA+AGG 0.470742 3.3:+22987602 MS.gene46686:CDS
GTCACAGATCTCATAGTTGA+TGG 0.474884 3.3:+22988343 MS.gene46686:CDS
ATGGAGCAGATAGAAAGCTT+TGG 0.483983 3.3:+22988305 MS.gene46686:CDS
TCAGTGTTGGGACTGTGTCC+TGG 0.491323 3.3:-22989188 None:intergenic
GGAATGAGGCATGTTCTAAA+GGG 0.495884 3.3:+22988055 MS.gene46686:CDS
TCATAACCAACAGCACCATT+GGG 0.496240 3.3:+22989243 MS.gene46686:CDS
ACACTAATAGGAACCACAAA+TGG 0.503421 3.3:+22990071 MS.gene46686:CDS
ATATGAATTCCATCAGTGTT+GGG 0.505639 3.3:-22989200 None:intergenic
GCAAGTGAATTCCTTTGCAT+GGG 0.507357 3.3:-22991461 None:intergenic
ACAATTTAGTTTCATGGCAA+GGG 0.511066 3.3:+22987603 MS.gene46686:CDS
AACATTGTCACATTTGGCAG+TGG 0.514203 3.3:-22991520 None:intergenic
AATATGAATTCCATCAGTGT+TGG 0.516408 3.3:-22989201 None:intergenic
TCTAGCATTGGAAGCTTAGG+AGG 0.516638 3.3:+22990002 MS.gene46686:intron
ATTGTATTTCAATTGTAAGT+GGG 0.522080 3.3:+22989794 MS.gene46686:CDS
TGAATGCAATAATTTGAAAG+TGG 0.522545 3.3:+22989076 MS.gene46686:CDS
ATGAACCTGATTCCATGTCC+TGG 0.522587 3.3:-22989853 None:intergenic
AATGCAGTATTGGGTTGGCA+TGG 0.531497 3.3:-22988138 None:intergenic
GATTGTATTTCAATTGTAAG+TGG 0.534722 3.3:+22989793 MS.gene46686:CDS
ATTTGTGGTTCCTATTAGTG+TGG 0.542336 3.3:-22990069 None:intergenic
ATTTGAAATGTCATGGAATG+AGG 0.544227 3.3:+22988041 MS.gene46686:CDS
ACCAACAGCACCATTGGGAC+AGG 0.545836 3.3:+22989248 MS.gene46686:CDS
TATGGAGCCAAATCTAATGA+TGG 0.549828 3.3:+22987685 MS.gene46686:CDS
TTCTAAAGGGAAGGTTCTTG+TGG 0.553586 3.3:+22988068 MS.gene46686:CDS
TGATGGACAAGTTGATAATG+AGG 0.564129 3.3:+22987702 MS.gene46686:CDS
AGTCAGAATAAAGACTTGGC+AGG 0.564393 3.3:+22990094 MS.gene46686:CDS
GCCAAAACACAAACCAAATG+AGG 0.566883 3.3:-22987506 None:intergenic
CGAGCAACAGATATTACTTG+TGG 0.574897 3.3:+22989829 MS.gene46686:CDS
TCAACCTTACAATGAGAGGT+TGG 0.581966 3.3:+22987564 MS.gene46686:CDS
AATCTAATAGTCACAGCACC+AGG 0.584074 3.3:+22989170 MS.gene46686:CDS
GGACACAGTCCCAACACTGA+TGG 0.584378 3.3:+22989191 MS.gene46686:CDS
ATTACTTGTGGACCAGGACA+TGG 0.589105 3.3:+22989841 MS.gene46686:CDS
ATCATCAACCTTACAATGAG+AGG 0.592562 3.3:+22987560 MS.gene46686:CDS
AGCAAAGCTGTCCCATGCAA+AGG 0.594681 3.3:+22991450 MS.gene46686:CDS
GATCTCATAGTTGATGGTGG+TGG 0.596400 3.3:+22988349 MS.gene46686:CDS
AGCATTGGAAGCTTAGGAGG+AGG 0.597724 3.3:+22990005 MS.gene46686:intron
ACAGATATTACTTGTGGACC+AGG 0.610780 3.3:+22989835 MS.gene46686:CDS
ATGGAGTCAGAATAAAGACT+TGG 0.611051 3.3:+22990090 MS.gene46686:CDS
AGAAGCTGAAGTGTCTAATG+TGG 0.616234 3.3:+22990034 MS.gene46686:CDS
TGAGGCATGTTCTAAAGGGA+AGG 0.623152 3.3:+22988059 MS.gene46686:CDS
CAAATTGCAGAATTCAGCTG+TGG 0.645778 3.3:+22991356 MS.gene46686:intron
TCTATGGAACCATCAAAGCA+TGG 0.658530 3.3:+22988266 MS.gene46686:CDS
GATGTGAAATTAACAGCACA+AGG 0.676145 3.3:+22991486 MS.gene46686:CDS
ACAGATCTCATAGTTGATGG+TGG 0.709690 3.3:+22988346 MS.gene46686:CDS
TTCGCCGCAATCTCAAAACA+CGG 0.711068 3.3:+22987648 MS.gene46686:CDS
TATTTCACAGCCATGCAAGG+AGG 0.712969 3.3:+22988930 MS.gene46686:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGTTAAAAAATTTGATA+TGG + chr3.3:22989925-22989944 MS.gene46686:intron 10.0%
!! GCTATTTATTAAATAAATTA+AGG + chr3.3:22990127-22990146 MS.gene46686:intron 10.0%
!!! AACAAAATAAATTTTTGAAA+AGG - chr3.3:22988603-22988622 None:intergenic 10.0%
!!! ATTTATGCTTTTATAAAATT+TGG + chr3.3:22991219-22991238 MS.gene46686:intron 10.0%
!!! ATTTTGAATTTGTTAAATTA+AGG + chr3.3:22988492-22988511 MS.gene46686:intron 10.0%
!!! GATTATTATTTTTAATTGAT+TGG + chr3.3:22989949-22989968 MS.gene46686:intron 10.0%
!!! TAATGGATTTTTTTTTATAA+TGG + chr3.3:22990352-22990371 MS.gene46686:intron 10.0%
!!! TATGGTTTATTTTATAATTT+TGG + chr3.3:22990247-22990266 MS.gene46686:intron 10.0%
!!! TTATTCAATTTAATTTTTCA+TGG + chr3.3:22989019-22989038 MS.gene46686:intron 10.0%
!! AAATTATAAAGTAAAAAGAG+TGG + chr3.3:22990159-22990178 MS.gene46686:intron 15.0%
!! AATGTTAATTATTCAAATAG+TGG + chr3.3:22991537-22991556 MS.gene46686:CDS 15.0%
!! AGGTAAATTAAAATGATTTA+TGG + chr3.3:22988163-22988182 MS.gene46686:intron 15.0%
!! ATTAAAATGAATGAAAACAT+GGG - chr3.3:22991144-22991163 None:intergenic 15.0%
!! GAAACTAAACAAATAAAAAA+AGG - chr3.3:22991177-22991196 None:intergenic 15.0%
!! GATTAAATGAATGAATTAAA+AGG - chr3.3:22988456-22988475 None:intergenic 15.0%
!! GCAAATAAACTAATAATAAA+TGG - chr3.3:22988804-22988823 None:intergenic 15.0%
!! TATTAAAATGAATGAAAACA+TGG - chr3.3:22991145-22991164 None:intergenic 15.0%
!! TGAATCAAATTAAAATGATA+AGG - chr3.3:22988872-22988891 None:intergenic 15.0%
!! TTAAAATATTTATGTGATCA+AGG + chr3.3:22987870-22987889 MS.gene46686:intron 15.0%
!!! AATTAGTGATTTGAATTTTA+TGG + chr3.3:22988824-22988843 MS.gene46686:intron 15.0%
!!! AATTAGTTATTTTCTTTACT+TGG - chr3.3:22988656-22988675 None:intergenic 15.0%
!!! ATATTTTTGAGTTATGATTA+TGG - chr3.3:22990843-22990862 None:intergenic 15.0%
!!! ATGAGTTTTATATATTTAAG+TGG - chr3.3:22988205-22988224 None:intergenic 15.0%
!!! TATTTTTGAGTTATGATTAT+GGG - chr3.3:22990842-22990861 None:intergenic 15.0%
!!! TCCTCAATATTTTTATAATT+AGG + chr3.3:22988685-22988704 MS.gene46686:intron 15.0%
!!! TTTTTATTTGTTTAGTTTCA+TGG + chr3.3:22991178-22991197 MS.gene46686:intron 15.0%
!! AAACAAATAAAAAAAGGCAA+GGG - chr3.3:22991171-22991190 None:intergenic 20.0%
!! AAACTAAATTGTTCATGTAT+TGG - chr3.3:22987595-22987614 None:intergenic 20.0%
!! AATGAAAAAAAACTCTTTGT+TGG - chr3.3:22990550-22990569 None:intergenic 20.0%
!! ATAATTAACATTGTCACATT+TGG - chr3.3:22991529-22991548 None:intergenic 20.0%
!! ATACTATATATCTAAATGCA+GGG + chr3.3:22990755-22990774 MS.gene46686:intron 20.0%
!! ATTGGTTAAAAAATTTGATG+TGG + chr3.3:22989967-22989986 MS.gene46686:intron 20.0%
!! ATTGTATTTCAATTGTAAGT+GGG + chr3.3:22989794-22989813 MS.gene46686:CDS 20.0%
!! ATTTAAAGAGATAAAAAGAG+TGG - chr3.3:22991256-22991275 None:intergenic 20.0%
!! GATATTTGTTTCCATTTAAA+TGG + chr3.3:22987985-22988004 MS.gene46686:intron 20.0%
!! GCCTAATTATAAAAATATTG+AGG - chr3.3:22988689-22988708 None:intergenic 20.0%
!! GTTAAACTTCATGTTAATTT+AGG + chr3.3:22990269-22990288 MS.gene46686:intron 20.0%
!! TAAACAAATAAAAAAAGGCA+AGG - chr3.3:22991172-22991191 None:intergenic 20.0%
!! TTAATGGAAATGGAAAAATA+TGG + chr3.3:22988377-22988396 MS.gene46686:CDS 20.0%
!!! ATGAAAATTTAACTTACTGT+TGG - chr3.3:22988958-22988977 None:intergenic 20.0%
!!! ATGCTTTTATAAAATTTGGT+TGG + chr3.3:22991223-22991242 MS.gene46686:intron 20.0%
!!! ATTTGAGTTAATATCATACA+AGG - chr3.3:22988432-22988451 None:intergenic 20.0%
!!! CAAATACACAATTTTACAAT+CGG - chr3.3:22987966-22987985 None:intergenic 20.0%
!!! TAATTATTGTTTTCTAGCAT+TGG + chr3.3:22989990-22990009 MS.gene46686:intron 20.0%
!!! TATTTTTCCATTTCCATTAA+TGG - chr3.3:22988377-22988396 None:intergenic 20.0%
!!! TGCTTTTATAAAATTTGGTT+GGG + chr3.3:22991224-22991243 MS.gene46686:intron 20.0%
!!! TTTTGAAAGATAAACGTTTT+TGG + chr3.3:22990714-22990733 MS.gene46686:intron 20.0%
! AAACTTTAACTAACCAAGTT+TGG + chr3.3:22990415-22990434 MS.gene46686:intron 25.0%
! AAAGGAGAGTAAAGAAATTA+TGG - chr3.3:22988910-22988929 None:intergenic 25.0%
! AAATTAACGAACAAAATCAG+AGG + chr3.3:22990956-22990975 MS.gene46686:intron 25.0%
! AACAAATAAAAAAAGGCAAG+GGG - chr3.3:22991170-22991189 None:intergenic 25.0%
! ACAAATTCAAAATCAATGCA+TGG - chr3.3:22988485-22988504 None:intergenic 25.0%
! ATATATTTAAGTGGACATTG+AGG - chr3.3:22988196-22988215 None:intergenic 25.0%
! ATCACTAATCACATTATGAT+TGG + chr3.3:22987911-22987930 MS.gene46686:intron 25.0%
! ATCATAATGTGATTAGTGAT+CGG - chr3.3:22987911-22987930 None:intergenic 25.0%
! ATTTACCTTTAATGCAGTAT+TGG - chr3.3:22988151-22988170 None:intergenic 25.0%
! ATTTGAGAACACCATTTAAA+TGG - chr3.3:22987999-22988018 None:intergenic 25.0%
! CATACTATATATCTAAATGC+AGG + chr3.3:22990754-22990773 MS.gene46686:intron 25.0%
! GATTGTATTTCAATTGTAAG+TGG + chr3.3:22989793-22989812 MS.gene46686:CDS 25.0%
! GTTAATGTTCATCAAGTATA+TGG - chr3.3:22990596-22990615 None:intergenic 25.0%
! GTTCAAATTAGTTAAGAAGA+AGG - chr3.3:22990528-22990547 None:intergenic 25.0%
! GTTTCATTATGTTAGATCTA+TGG + chr3.3:22988250-22988269 MS.gene46686:intron 25.0%
! TATATTTAAGTGGACATTGA+GGG - chr3.3:22988195-22988214 None:intergenic 25.0%
! TATGAAAACATTCTCTGAAT+GGG - chr3.3:22989228-22989247 None:intergenic 25.0%
! TGAATGCAATAATTTGAAAG+TGG + chr3.3:22989076-22989095 MS.gene46686:CDS 25.0%
! TGCATTAGCACTATTATATT+GGG + chr3.3:22990303-22990322 MS.gene46686:intron 25.0%
! TTATGAAAACATTCTCTGAA+TGG - chr3.3:22989229-22989248 None:intergenic 25.0%
! TTGTAAAATCGTGTATTTGT+TGG + chr3.3:22987939-22987958 MS.gene46686:intron 25.0%
! TTTACCTTTAATGCAGTATT+GGG - chr3.3:22988150-22988169 None:intergenic 25.0%
!! ACATGAACAATTTAGTTTCA+TGG + chr3.3:22987597-22987616 MS.gene46686:CDS 25.0%
!! CAAATACACGATTTTACAAT+CGG - chr3.3:22987938-22987957 None:intergenic 25.0%
!! TAAAGCCTATAACATTTTCA+GGG + chr3.3:22988115-22988134 MS.gene46686:CDS 25.0%
!! TATTTTACCAAATCATCTCA+AGG + chr3.3:22990212-22990231 MS.gene46686:intron 25.0%
!! TGTTAATTTAGGACCAAATT+AGG + chr3.3:22990280-22990299 MS.gene46686:intron 25.0%
!! TTGACATGTTTGAAGTAATA+AGG - chr3.3:22990469-22990488 None:intergenic 25.0%
!!! ATTTCTCATTTTTGCACTTT+AGG + chr3.3:22988017-22988036 MS.gene46686:intron 25.0%
!!! GGAAAATTTGTTGCATATAT+AGG + chr3.3:22989040-22989059 MS.gene46686:intron 25.0%
!!! TTAAGACAAGAAGTCATTTT+AGG + chr3.3:22989658-22989677 MS.gene46686:intron 25.0%
AAAAAAGAAATGTCAAGAGC+AGG + chr3.3:22990871-22990890 MS.gene46686:CDS 30.0%
AACTTGTCCATCATTAGATT+TGG - chr3.3:22987695-22987714 None:intergenic 30.0%
AATATGAATTCCATCAGTGT+TGG - chr3.3:22989204-22989223 None:intergenic 30.0%
AATGTTACATGGACTCATAA+TGG + chr3.3:22990335-22990354 MS.gene46686:intron 30.0%
AATTTAGCGAAAACAATGTG+TGG + chr3.3:22987726-22987745 MS.gene46686:intron 30.0%
ACAAATAAAAAAAGGCAAGG+GGG - chr3.3:22991169-22991188 None:intergenic 30.0%
AGATGATAGACACTATGTTT+AGG - chr3.3:22988096-22988115 None:intergenic 30.0%
ATATGAATTCCATCAGTGTT+GGG - chr3.3:22989203-22989222 None:intergenic 30.0%
ATGCACAACAAATGCATATA+AGG + chr3.3:22989117-22989136 MS.gene46686:CDS 30.0%
ATGGAAATGGAAAAATATGG+TGG + chr3.3:22988380-22988399 MS.gene46686:CDS 30.0%
ATTATGAGTCCATGTAACAT+TGG - chr3.3:22990336-22990355 None:intergenic 30.0%
CAAATGCATATAAGGATTGA+AGG + chr3.3:22989125-22989144 MS.gene46686:CDS 30.0%
GAAGTAATAAGGTTAGAAAC+TGG - chr3.3:22990458-22990477 None:intergenic 30.0%
GTGATGTACCAAAACATAAA+AGG + chr3.3:22991390-22991409 MS.gene46686:CDS 30.0%
GTGCATTAGCACTATTATAT+TGG + chr3.3:22990302-22990321 MS.gene46686:intron 30.0%
TAGACCTAAAACTCAAGTTT+AGG - chr3.3:22990988-22991007 None:intergenic 30.0%
TCAAGGACTTCAAGTAAATA+TGG + chr3.3:22990229-22990248 MS.gene46686:intron 30.0%
TGGAAATGGAAAAATATGGT+GGG + chr3.3:22988381-22988400 MS.gene46686:CDS 30.0%
TTGAATGTCACTCTACAAAA+CGG - chr3.3:22987844-22987863 None:intergenic 30.0%
! AACAATTTAGTTTCATGGCA+AGG + chr3.3:22987602-22987621 MS.gene46686:CDS 30.0%
! ACAACCTAAACTTGAGTTTT+AGG + chr3.3:22990981-22991000 MS.gene46686:intron 30.0%
! ACAATTTAGTTTCATGGCAA+GGG + chr3.3:22987603-22987622 MS.gene46686:CDS 30.0%
! ATTTGAAATGTCATGGAATG+AGG + chr3.3:22988041-22988060 MS.gene46686:CDS 30.0%
! CACTTGTTCCTTTTATGTTT+TGG - chr3.3:22991401-22991420 None:intergenic 30.0%
! CTAAAGCCTATAACATTTTC+AGG + chr3.3:22988114-22988133 MS.gene46686:CDS 30.0%
! TTTAGGCATTTGAAATGTCA+TGG + chr3.3:22988034-22988053 MS.gene46686:intron 30.0%
!! ATTCCTCACTATTTCTGTTA+AGG + chr3.3:22989766-22989785 MS.gene46686:intron 30.0%
!!! GCTCTTGACATTTCTTTTTT+TGG - chr3.3:22990871-22990890 None:intergenic 30.0%
AATATTAGGCCATGCTTTGA+TGG - chr3.3:22988278-22988297 None:intergenic 35.0%
AATGAATGAAAACATGGGCT+TGG - chr3.3:22991139-22991158 None:intergenic 35.0%
ACACTAATAGGAACCACAAA+TGG + chr3.3:22990071-22990090 MS.gene46686:CDS 35.0%
AGACTTGGCAGGTAAATTTA+TGG + chr3.3:22990105-22990124 MS.gene46686:intron 35.0%
ATCATCAACCTTACAATGAG+AGG + chr3.3:22987560-22987579 MS.gene46686:CDS 35.0%
ATGGAGTCAGAATAAAGACT+TGG + chr3.3:22990090-22990109 MS.gene46686:CDS 35.0%
ATTTGTGGTTCCTATTAGTG+TGG - chr3.3:22990072-22990091 None:intergenic 35.0%
CAAACCAAATGAGGCTAAAA+TGG - chr3.3:22987500-22987519 None:intergenic 35.0%
CAAATAAAAAAAGGCAAGGG+GGG - chr3.3:22991168-22991187 None:intergenic 35.0%
CTATATATCTAAATGCAGGG+AGG + chr3.3:22990758-22990777 MS.gene46686:intron 35.0%
CTTTATTCTGACTCCATTTG+TGG - chr3.3:22990087-22990106 None:intergenic 35.0%
CTTTGTAAACTTGCCAAACT+TGG - chr3.3:22990431-22990450 None:intergenic 35.0%
GATGTGAAATTAACAGCACA+AGG + chr3.3:22991486-22991505 MS.gene46686:CDS 35.0%
GCTCCATATGCTGAAATATT+AGG - chr3.3:22988292-22988311 None:intergenic 35.0%
TAGTGCTAATGCACCTAATT+TGG - chr3.3:22990296-22990315 None:intergenic 35.0%
TATAGAGCAACACATGATGT+GGG + chr3.3:22991030-22991049 MS.gene46686:intron 35.0%
TATGGAGCCAAATCTAATGA+TGG + chr3.3:22987685-22987704 MS.gene46686:CDS 35.0%
TCACCTTAACAGAAATAGTG+AGG - chr3.3:22989772-22989791 None:intergenic 35.0%
TGAAGGATGCAACAATGTTT+GGG + chr3.3:22989142-22989161 MS.gene46686:CDS 35.0%
TGGCCTAATATTTCAGCATA+TGG + chr3.3:22988286-22988305 MS.gene46686:CDS 35.0%
TTGAAGGATGCAACAATGTT+TGG + chr3.3:22989141-22989160 MS.gene46686:CDS 35.0%
TTGAAGTCCTTGAGATGATT+TGG - chr3.3:22990222-22990241 None:intergenic 35.0%
TTGCATGGCTGTGAAATAAA+AGG - chr3.3:22988928-22988947 None:intergenic 35.0%
! ATCTGTTGCTCGAACATTTT+TGG - chr3.3:22989821-22989840 None:intergenic 35.0%
! TATCTTTTTACCTGTCCCAA+TGG - chr3.3:22989261-22989280 None:intergenic 35.0%
! TTGGCTCTTACTTAGAAGAA+AGG + chr3.3:22987524-22987543 MS.gene46686:CDS 35.0%
!! TGATGGACAAGTTGATAATG+AGG + chr3.3:22987702-22987721 MS.gene46686:CDS 35.0%
!! TTCACCATTTTAGCCTCATT+TGG + chr3.3:22987493-22987512 MS.gene46686:CDS 35.0%
!! TTTTATTTCACAGCCATGCA+AGG + chr3.3:22988927-22988946 MS.gene46686:intron 35.0%
!! TTTTCTAGCATTGGAAGCTT+AGG + chr3.3:22989999-22990018 MS.gene46686:intron 35.0%
!!! TTAACCGTGTTTTGAGATTG+CGG - chr3.3:22987655-22987674 None:intergenic 35.0%
AACATTGTCACATTTGGCAG+TGG - chr3.3:22991523-22991542 None:intergenic 40.0%
AATCTAATAGTCACAGCACC+AGG + chr3.3:22989170-22989189 MS.gene46686:CDS 40.0%
ACAGATATTACTTGTGGACC+AGG + chr3.3:22989835-22989854 MS.gene46686:CDS 40.0%
AGTCAGAATAAAGACTTGGC+AGG + chr3.3:22990094-22990113 MS.gene46686:CDS 40.0%
ATAGAGCAACACATGATGTG+GGG + chr3.3:22991031-22991050 MS.gene46686:intron 40.0%
ATGGAGCAGATAGAAAGCTT+TGG + chr3.3:22988305-22988324 MS.gene46686:CDS 40.0%
CAAATTGCAGAATTCAGCTG+TGG + chr3.3:22991356-22991375 MS.gene46686:intron 40.0%
CATGGCCCTGAAAATGTTAT+AGG - chr3.3:22988123-22988142 None:intergenic 40.0%
CCAACCCAATACTGCATTAA+AGG + chr3.3:22988143-22988162 MS.gene46686:CDS 40.0%
CGAGCAACAGATATTACTTG+TGG + chr3.3:22989829-22989848 MS.gene46686:CDS 40.0%
GCAAGTGAATTCCTTTGCAT+GGG - chr3.3:22991464-22991483 None:intergenic 40.0%
GCCAAAACACAAACCAAATG+AGG - chr3.3:22987509-22987528 None:intergenic 40.0%
GGAAGTTGACCAATGTTACA+TGG + chr3.3:22990324-22990343 MS.gene46686:intron 40.0%
GGTGGTACCATTAATGGAAA+TGG + chr3.3:22988367-22988386 MS.gene46686:CDS 40.0%
GTATAGAGCAACACATGATG+TGG + chr3.3:22991029-22991048 MS.gene46686:intron 40.0%
GTCACAGATCTCATAGTTGA+TGG + chr3.3:22988343-22988362 MS.gene46686:CDS 40.0%
TCAACCTTACAATGAGAGGT+TGG + chr3.3:22987564-22987583 MS.gene46686:CDS 40.0%
TCATAACCAACAGCACCATT+GGG + chr3.3:22989243-22989262 MS.gene46686:CDS 40.0%
TCTATGGAACCATCAAAGCA+TGG + chr3.3:22988266-22988285 MS.gene46686:CDS 40.0%
TGCAAGTGAATTCCTTTGCA+TGG - chr3.3:22991465-22991484 None:intergenic 40.0%
TGGAATGAGGCATGTTCTAA+AGG + chr3.3:22988054-22988073 MS.gene46686:CDS 40.0%
TGTGATTAGTGATCGGAACA+AGG - chr3.3:22987904-22987923 None:intergenic 40.0%
TTCACCAACCTCTCATTGTA+AGG - chr3.3:22987571-22987590 None:intergenic 40.0%
TTCAGAGCAACACAAGATGT+GGG + chr3.3:22991055-22991074 MS.gene46686:intron 40.0%
TTCATAACCAACAGCACCAT+TGG + chr3.3:22989242-22989261 MS.gene46686:CDS 40.0%
TTTCAGAGCAACACAAGATG+TGG + chr3.3:22991054-22991073 MS.gene46686:intron 40.0%
! AGAAGCTGAAGTGTCTAATG+TGG + chr3.3:22990034-22990053 MS.gene46686:CDS 40.0%
! CCTTTAATGCAGTATTGGGT+TGG - chr3.3:22988146-22988165 None:intergenic 40.0%
! GGAATGAGGCATGTTCTAAA+GGG + chr3.3:22988055-22988074 MS.gene46686:CDS 40.0%
! TTCTAAAGGGAAGGTTCTTG+TGG + chr3.3:22988068-22988087 MS.gene46686:CDS 40.0%
!! ACAGATCTCATAGTTGATGG+TGG + chr3.3:22988346-22988365 MS.gene46686:CDS 40.0%
!!! GCCTCATTTGGTTTGTGTTT+TGG + chr3.3:22987505-22987524 MS.gene46686:CDS 40.0%
ACGGTTAACGTTGACGACTA+TGG + chr3.3:22987667-22987686 MS.gene46686:CDS 45.0%
ATCTAAATGCAGGGAGGTTC+TGG + chr3.3:22990764-22990783 MS.gene46686:intron 45.0%
ATGAACCTGATTCCATGTCC+TGG - chr3.3:22989856-22989875 None:intergenic 45.0%
ATTACTTGTGGACCAGGACA+TGG + chr3.3:22989841-22989860 MS.gene46686:CDS 45.0%
GTGAACAGAGCCACACTAAT+AGG + chr3.3:22990059-22990078 MS.gene46686:CDS 45.0%
TATTTCACAGCCATGCAAGG+AGG + chr3.3:22988930-22988949 MS.gene46686:intron 45.0%
TCAGAGCAACACAAGATGTG+GGG + chr3.3:22991056-22991075 MS.gene46686:intron 45.0%
TTCGCCGCAATCTCAAAACA+CGG + chr3.3:22987648-22987667 MS.gene46686:CDS 45.0%
! TCTAGCATTGGAAGCTTAGG+AGG + chr3.3:22990002-22990021 MS.gene46686:intron 45.0%
!! AATGCAGTATTGGGTTGGCA+TGG - chr3.3:22988141-22988160 None:intergenic 45.0%
!! GATCTCATAGTTGATGGTGG+TGG + chr3.3:22988349-22988368 MS.gene46686:CDS 45.0%
!! GATGGTGGTGGTACCATTAA+TGG + chr3.3:22988361-22988380 MS.gene46686:CDS 45.0%
!! TGAGGCATGTTCTAAAGGGA+AGG + chr3.3:22988059-22988078 MS.gene46686:CDS 45.0%
!! AAATAAAAAATTAAATTAAG+TGG - chr3.3:22990197-22990216 None:intergenic 5.0%
!! AAATTTATTTACAAATTAAA+AGG - chr3.3:22988715-22988734 None:intergenic 5.0%
!! AACTATTTATTATTATTTAT+TGG - chr3.3:22989720-22989739 None:intergenic 5.0%
!! AATAAAAAATTAAATTAAGT+GGG - chr3.3:22990196-22990215 None:intergenic 5.0%
!! ACTATTTATTATTATTTATT+GGG - chr3.3:22989719-22989738 None:intergenic 5.0%
CAGAGCAACACAAGATGTGG+GGG + chr3.3:22991057-22991076 MS.gene46686:intron 50.0%
! AGCATTGGAAGCTTAGGAGG+AGG + chr3.3:22990005-22990024 MS.gene46686:intron 50.0%
!!! GTGTTTTGAGATTGCGGCGA+AGG - chr3.3:22987649-22987668 None:intergenic 50.0%
ACCAACAGCACCATTGGGAC+AGG + chr3.3:22989248-22989267 MS.gene46686:CDS 55.0%
GGACACAGTCCCAACACTGA+TGG + chr3.3:22989191-22989210 MS.gene46686:CDS 55.0%
GTGGACCAGGACATGGAATC+AGG + chr3.3:22989848-22989867 MS.gene46686:CDS 55.0%
! ACTGTTGGTGCCTCCTTGCA+TGG - chr3.3:22988943-22988962 None:intergenic 55.0%
! TCAGTGTTGGGACTGTGTCC+TGG - chr3.3:22989191-22989210 None:intergenic 55.0%
!! ACCTGTCCCAATGGTGCTGT+TGG - chr3.3:22989252-22989271 None:intergenic 55.0%
! TGCGGCGAAGGCGCGATAGA+TGG - chr3.3:22987637-22987656 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 22987454 22991587 22987454 ID=MS.gene46686
chr3.3 mRNA 22987454 22991587 22987454 ID=MS.gene46686.t1;Parent=MS.gene46686
chr3.3 exon 22987454 22987723 22987454 ID=MS.gene46686.t1.exon1;Parent=MS.gene46686.t1
chr3.3 CDS 22987454 22987723 22987454 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22988039 22988164 22988039 ID=MS.gene46686.t1.exon2;Parent=MS.gene46686.t1
chr3.3 CDS 22988039 22988164 22988039 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22988265 22988432 22988265 ID=MS.gene46686.t1.exon3;Parent=MS.gene46686.t1
chr3.3 CDS 22988265 22988432 22988265 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22988940 22988960 22988940 ID=MS.gene46686.t1.exon4;Parent=MS.gene46686.t1
chr3.3 CDS 22988940 22988960 22988940 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22989062 22989269 22989062 ID=MS.gene46686.t1.exon5;Parent=MS.gene46686.t1
chr3.3 CDS 22989062 22989269 22989062 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22989788 22989869 22989788 ID=MS.gene46686.t1.exon6;Parent=MS.gene46686.t1
chr3.3 CDS 22989788 22989869 22989788 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22990007 22990115 22990007 ID=MS.gene46686.t1.exon7;Parent=MS.gene46686.t1
chr3.3 CDS 22990007 22990115 22990007 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22990776 22990892 22990776 ID=MS.gene46686.t1.exon8;Parent=MS.gene46686.t1
chr3.3 CDS 22990776 22990892 22990776 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
chr3.3 exon 22991366 22991587 22991366 ID=MS.gene46686.t1.exon9;Parent=MS.gene46686.t1
chr3.3 CDS 22991366 22991587 22991366 ID=cds.MS.gene46686.t1;Parent=MS.gene46686.t1
Gene Sequence

>MS.gene46686

ATGAGAAAGATGTATTCACAAAGCATAATTCTTTCACTTTTCACCATTTTAGCCTCATTTGGTTTGTGTTTTGGCTCTTACTTAGAAGAAAGGCATTATTTAACAAATCATCAACCTTACAATGAGAGGTTGGTGAAGCCAATACATGAACAATTTAGTTTCATGGCAAGGGCTCATCATCCATCTATCGCGCCTTCGCCGCAATCTCAAAACACGGTTAACGTTGACGACTATGGAGCCAAATCTAATGATGGACAAGTTGATAATGAGGTAATTTAGCGAAAACAATGTGTGGTATATTTTTACTGAAGTTGTATTTGAATCTCACGTTGTCATGATTCATGAGTTATGCAATGCTTATATGTGTTTTTGTCTCATCTTACAAGTCCGTTTTGTAGAGTGACATTCAAATTCATTTAAAATATTTATGTGATCAAGGTTTAACTGCCTTGTTCCGATCACTAATCACATTATGATTGGTCCGATTGTAAAATCGTGTATTTGTTGGTCCGATTGTAAAATTGTGTATTTGATATTTGTTTCCATTTAAATGGTGTTCTCAAATTTCTCATTTTTGCACTTTAGGCATTTGAAATGTCATGGAATGAGGCATGTTCTAAAGGGAAGGTTCTTGTGGTACCTAAACATAGTGTCTATCATCTAAAGCCTATAACATTTTCAGGGCCATGCCAACCCAATACTGCATTAAAGGTAAATTAAAATGATTTATGGTTATAACCCTCAATGTCCACTTAAATATATAAAACTCATTGTAACGTTTTTGTGTTTAATTACTGTTTCATTATGTTAGATCTATGGAACCATCAAAGCATGGCCTAATATTTCAGCATATGGAGCAGATAGAAAGCTTTGGATCAAGTTTGAGAATGTCACAGATCTCATAGTTGATGGTGGTGGTACCATTAATGGAAATGGAAAAATATGGTGGGAGAATTCTTGCAAAGTGAATGAAAGCCTTGTATGATATTAACTCAAATTCCTTTTAATTCATTCATTTAATCTTTAATCCATGCATTGATTTTGAATTTGTTAAATTAAGGAAAAATATATTTTGTCATTTTTTGTTTCTTGTTTACTTTTTAGTATTAACTAAAAATCGTATGCTATTATACACTTTAATTTTTTCCTTTTCAAAAATTTATTTTGTTTATACAAACATAAAGAAAATAACTTTTTTGCCAAGTAAAGAAAATAACTAATTACTCACTTTTCCTCAATATTTTTATAATTAGGCTCGCCTTTTAATTTGTAAATAAATTTTTTTTACAAATAAAGAAAATAACTTACAAAGAAACATAAAGAATTGTTTTTAAGAAATTTTAATAACCATTTATTATTAGTTTATTTGCAATTAGTGATTTGAATTTTATGGAATCATTATATAGACTTTTTTTCCTTATCATTTTAATTTGATTCAAATTTTAAATACTGACCATAATTTCTTTACTCTCCTTTTATTTCACAGCCATGCAAGGAGGCACCAACAGTAAGTTAAATTTTCATTTTTGTGAACTTTATTACATGCTTTTCTGAAATACTCATCTTTATTCAATTTAATTTTTCATGGAAAATTTGTTGCATATATAGGCTGTGACTTTCGATGAATGCAATAATTTGAAAGTGGATAAGATAAGATTCAGAAATGCACAACAAATGCATATAAGGATTGAAGGATGCAACAATGTTTGGGCATCAAATCTAATAGTCACAGCACCAGGACACAGTCCCAACACTGATGGAATTCATATTACCCATTCAGAGAATGTTTTCATAACCAACAGCACCATTGGGACAGGTAAAAAGATATATAAATGAAAAAAAATGTCACATTATAATATCATTTTTCATAATTTATGTGTTTTCATATTATAATATCATTTTTTTAAAATCATAACTAATTATATGATGAAAAATTATTAGACGTTACTATAGTAGAGTAATTTTGCATTGTTTGTGCATAATAATTAAGTAAATCAAAGATTAGATTATGTACAATTTATTTATTTTTTTAAAGAATTATGCACAATTTCTAAACAAATTATTCAAATGTATAATTTTTCTTAAAAAATATGTAGCATTTGTATTTTCTTTAAATGACACACGTACGTATGTATGTATGTATGTATGTATGTACTCTAATCATTAAAAATGAATTATTATTTTTATTTTTATAGAAACTAACTTTAAGACAAGAAGTCATTTTAGGAAGTAATAAACAAAACATCTATTATAACTTTATAACCCAATAAATAATAATAAATAGTTACTTGAATTTTTCAATTTTCTAATTTATTCCTCACTATTTCTGTTAAGGTGATGATTGTATTTCAATTGTAAGTGGGTCCAAAAATGTTCGAGCAACAGATATTACTTGTGGACCAGGACATGGAATCAGGTTCATGACTCTCTTAGTTGAAGTTTTTTTTTTTCTCTCGTTGAATTTTAAATTGATTAGTTAAAAAATTTGATATGGTGATTATTATTTTTAATTGATTGGTTAAAAAATTTGATGTGGTAATTATTGTTTTCTAGCATTGGAAGCTTAGGAGGAGGTAACACAGAAGCTGAAGTGTCTAATGTGGAAGTGAACAGAGCCACACTAATAGGAACCACAAATGGAGTCAGAATAAAGACTTGGCAGGTAAATTTATGGCTATTTATTAAATAAATTAAGGCTCATTTATAAATTATAAAGTAAAAAGAGTGGAATTAAAGCTACCCACTTAATTTAATTTTTTATTTTACCAAATCATCTCAAGGACTTCAAGTAAATATGGTTTATTTTATAATTTTGGTTAAACTTCATGTTAATTTAGGACCAAATTAGGTGCATTAGCACTATTATATTGGGAAGTTGACCAATGTTACATGGACTCATAATGGATTTTTTTTTATAATGGTTGATTTTCATTTCTAACTAAACATAAAGTTTGACAAGCAAAACTTTAACTAACCAAGTTTGGCAAGTTTACAAAGTCTACCAGTTTCTAACCTTATTACTTCAAACATGTCAATAACATTTTATATTGTGTTGTTCAACTTTCTTTAGTCCTTCTTCTTAACTAATTTGAACCAACAAAGAGTTTTTTTTCATTTTTTTTTTTATAATTTTAGATGTCCATATACTTGATGAACATTAACTTTGACTAGACTATAAGAATGTATTTAAAAAAATTGATAAAAATTTATTCATTGTTATATTAGTAGTGTAGAGCTTAACTTGTTAGCTTTACTATTGATTTTGAAAGATAAACGTTTTTGGTCTAATGTCTTATTTTTCATACTATATATCTAAATGCAGGGAGGTTCTGGCTATGCAAGAAACATCAAATTTATGAACATAGTCATGCGAAATGTGACTAATCCCATAATCATAACTCAAAAATATTGTGACCAAAAAAAGAAATGTCAAGAGCAGGTAATTGTTTTTAAATGTCTTAACTACTTTTATTTTTTATATTATTTAAAGAAGAAAAAAATAAAATTAACGAACAAAATCAGAGGAAACAACCTAAACTTGAGTTTTAGGTCTAAAAACAAATTATCAAAAATATGTATAGAGCAACACATGATGTGGGGTTTCAGAGCAACACAAGATGTGGGGGTATAAAGAGTAATTATCTATAAAAAAAATTCTTTTTTGTTTTGCAATATGTTTTCACCAAGCCCATGTTTTCATTCATTTTAATACCCCCCTTGCCTTTTTTTATTTGTTTAGTTTCATGGTTCAAAGTTCAAACTACAATTTATGCTTTTATAAAATTTGGTTGGGTTTAGTCCACTCTTTTTATCTCTTTAAATATTTGTTACTAATTTATTTTTTTTTAAATGTAGTTATAATACTGTTTAAAATTTGTTACTAATATAATCAAAATCATGATCAAATTGCAGAATTCAGCTGTGGAATTGAGCAATGTGATGTACCAAAACATAAAAGGAACAAGTGCTTCAGAAGTTGCAATCAAACTTGAATGTAGCAAAGCTGTCCCATGCAAAGGAATTCACTTGCAAGATGTGAAATTAACAGCACAAGGCAATGATAACACCACTGCCAAATGTGACAATGTTAATTATTCAAATAGTGGAATGTTCTTTCCTAAGTGTTACCCATGA
Protein sequence

>MS.gene46686.t1

MRKMYSQSIILSLFTILASFGLCFGSYLEERHYLTNHQPYNERLVKPIHEQFSFMARAHHPSIAPSPQSQNTVNVDDYGAKSNDGQVDNEAFEMSWNEACSKGKVLVVPKHSVYHLKPITFSGPCQPNTALKIYGTIKAWPNISAYGADRKLWIKFENVTDLIVDGGGTINGNGKIWWENSCKVNESLPCKEAPTAVTFDECNNLKVDKIRFRNAQQMHIRIEGCNNVWASNLIVTAPGHSPNTDGIHITHSENVFITNSTIGTGDDCISIVSGSKNVRATDITCGPGHGISIGSLGGGNTEAEVSNVEVNRATLIGTTNGVRIKTWQGGSGYARNIKFMNIVMRNVTNPIIITQKYCDQKKKCQEQNSAVELSNVMYQNIKGTSASEVAIKLECSKAVPCKGIHLQDVKLTAQGNDNTTAKCDNVNYSNSGMFFPKCYP