Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47310.t1 | XP_013451545.1 | 98.2 | 171 | 3 | 0 | 62 | 232 | 17 | 187 | 6.60E-64 | 254.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47310.t1 | Q9SE95 | 69.0 | 158 | 46 | 1 | 62 | 219 | 16 | 170 | 4.4e-40 | 166.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47310.t1 | A0A072U8Z3 | 98.2 | 171 | 3 | 0 | 62 | 232 | 17 | 187 | 4.8e-64 | 254.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050359 | MS.gene47310 | 0.803526 | 3.34E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene47310 | 0.804435 | 2.16E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene47310 | 0.821731 | 3.45E-53 | -1.69E-46 |
| MS.gene051003 | MS.gene47310 | 0.80959 | 1.75E-50 | -1.69E-46 |
| MS.gene051233 | MS.gene47310 | 0.805644 | 1.21E-49 | -1.69E-46 |
| MS.gene051234 | MS.gene47310 | 0.823802 | 1.14E-53 | -1.69E-46 |
| MS.gene052072 | MS.gene47310 | 0.832041 | 1.18E-55 | -1.69E-46 |
| MS.gene052296 | MS.gene47310 | 0.815375 | 9.55E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene47310 | 0.801251 | 9.83E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene47310 | 0.80338 | 3.58E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene47310 | MS.gene53384 | PPI |
| MS.gene47310 | MS.gene09068 | PPI |
| MS.gene023557 | MS.gene47310 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47310.t1 | MTR_6g027790 | 99.301 | 286 | 2 | 0 | 62 | 347 | 17 | 302 | 0.0 | 584 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47310.t1 | AT5G55000 | 81.469 | 286 | 50 | 1 | 62 | 347 | 16 | 298 | 1.79e-172 | 481 |
| MS.gene47310.t1 | AT5G55000 | 82.528 | 269 | 44 | 1 | 62 | 330 | 16 | 281 | 2.79e-164 | 460 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCGAGGGGTTAATCTTTC+TGG | 0.140143 | 6.4:-3151086 | MS.gene47310:CDS |
| CTTCGATTCGCTCATGTAAT+TGG | 0.162821 | 6.4:-3157637 | MS.gene47310:CDS |
| AAATCTTCGTGGTGCTTTAT+TGG | 0.215956 | 6.4:-3149936 | MS.gene47310:CDS |
| CTTCTTCCTTCCTCTTATTA+AGG | 0.218270 | 6.4:+3151174 | None:intergenic |
| TCTTCAATGTGCAAAGTTTC+GGG | 0.233983 | 6.4:-3150500 | MS.gene47310:intron |
| TTAGGGATTATGTACGTTAT+TGG | 0.239272 | 6.4:+3153668 | None:intergenic |
| ATCTTCAATGTGCAAAGTTT+CGG | 0.272003 | 6.4:-3150501 | MS.gene47310:CDS |
| ATTCACTGGTGTGTCCTATT+TGG | 0.277024 | 6.4:-3155082 | MS.gene47310:CDS |
| CTTCAAGACCGTTGGACTTT+CGG | 0.300997 | 6.4:+3155031 | None:intergenic |
| TAGGGATTATGTACGTTATT+GGG | 0.310696 | 6.4:+3153669 | None:intergenic |
| TCGCAGGAGGAAAGAAATTC+TGG | 0.315051 | 6.4:-3153776 | MS.gene47310:intron |
| CTTCGTGGTGCTTTATTGGC+TGG | 0.321965 | 6.4:-3149932 | MS.gene47310:CDS |
| GACACATTCTCAATTGGTTA+AGG | 0.327644 | 6.4:-3151940 | MS.gene47310:CDS |
| CAAACCAACAATAAGTCTTT+TGG | 0.327762 | 6.4:+3155107 | None:intergenic |
| TAGGGAAGATGTGAATTTAC+TGG | 0.329065 | 6.4:-3149962 | MS.gene47310:intron |
| CTGAATATTCAGAGCTGTTA+AGG | 0.330521 | 6.4:-3151889 | MS.gene47310:CDS |
| GTTTCAGATGCTTGTTTAGT+AGG | 0.336228 | 6.4:-3149415 | MS.gene47310:intron |
| GGATTTCACGTCTTCCAAAT+AGG | 0.341111 | 6.4:+3155068 | None:intergenic |
| TCCTCTTATTAAGGACATCT+TGG | 0.341120 | 6.4:+3151183 | None:intergenic |
| TCTGATTGCTCCGAGCAAAT+TGG | 0.346394 | 6.4:+3146031 | None:intergenic |
| TGGGATCTTGAGAGAGAGTA+TGG | 0.355414 | 6.4:+3153688 | None:intergenic |
| CAATCTGGAGGGAGCTAATC+TGG | 0.361067 | 6.4:-3147829 | MS.gene47310:CDS |
| GGAAAGATTCGTGCATTGAT+TGG | 0.392628 | 6.4:+3157662 | None:intergenic |
| TTCGATTCGCTCATGTAATT+GGG | 0.397718 | 6.4:-3157636 | MS.gene47310:CDS |
| AAATGTTAGGGGCTTATAGA+TGG | 0.404279 | 6.4:-3151209 | MS.gene47310:intron |
| ACACATTCTCAATTGGTTAA+GGG | 0.428609 | 6.4:-3151939 | MS.gene47310:CDS |
| TGGCATCGACCTCTCTAAAC+TGG | 0.430625 | 6.4:-3151066 | MS.gene47310:intron |
| ACTTCAGACACATTCTCAAT+TGG | 0.430939 | 6.4:-3151946 | MS.gene47310:CDS |
| CGAAAGTCCAACGGTCTTGA+AGG | 0.439720 | 6.4:-3155030 | MS.gene47310:intron |
| ATTGTTGGTTTGACATTCAC+TGG | 0.441613 | 6.4:-3155096 | MS.gene47310:CDS |
| CAGATTAGCTCCCTCCAGAT+TGG | 0.453075 | 6.4:+3147830 | None:intergenic |
| TTCGTGGTGCTTTATTGGCT+GGG | 0.455181 | 6.4:-3149931 | MS.gene47310:CDS |
| TTGGAAGGAGCAAAGCTTGA+TGG | 0.460846 | 6.4:-3146056 | MS.gene47310:CDS |
| GTATACAATCTGAGAGAGTC+AGG | 0.465422 | 6.4:-3151109 | MS.gene47310:CDS |
| GAGACCAAAAGACTTATTGT+TGG | 0.474268 | 6.4:-3155111 | MS.gene47310:CDS |
| TGATCTTACTAATGCCAATC+TGG | 0.476212 | 6.4:-3147844 | MS.gene47310:CDS |
| TGAATATTCAGAGCTGTTAA+GGG | 0.481904 | 6.4:-3151888 | MS.gene47310:CDS |
| GACGCAGATAAGCTCGTTGA+AGG | 0.487337 | 6.4:+3146916 | None:intergenic |
| CCTGCGATCTGCACATCTAC+AGG | 0.491495 | 6.4:-3149371 | MS.gene47310:intron |
| ATAGCAGCGAGCATTGAATC+AGG | 0.492744 | 6.4:+3153723 | None:intergenic |
| TCCAAGATGTCCTTAATAAG+AGG | 0.492750 | 6.4:-3151184 | MS.gene47310:CDS |
| CAAAGTTGCATTATGAAAGA+TGG | 0.497185 | 6.4:+3150062 | None:intergenic |
| TTAGTAGGATGTAGCTTCTG+TGG | 0.501874 | 6.4:-3149400 | MS.gene47310:CDS |
| TCTGAGAGAGTCAGGTTTCG+AGG | 0.507145 | 6.4:-3151101 | MS.gene47310:CDS |
| TCAACCACCTTCAAGACCGT+TGG | 0.511598 | 6.4:+3155023 | None:intergenic |
| AGGGAAGATGTGAATTTACT+GGG | 0.512755 | 6.4:-3149961 | MS.gene47310:intron |
| CTTGATGGTGCCAATTTGCT+CGG | 0.516763 | 6.4:-3146041 | MS.gene47310:CDS |
| CTTCAAAGCGCAAATCTACA+AGG | 0.519894 | 6.4:-3149902 | MS.gene47310:intron |
| CTGAGAGAGTCAGGTTTCGA+GGG | 0.520649 | 6.4:-3151100 | MS.gene47310:CDS |
| CACAAGTAGCTGATAGTACT+CGG | 0.521027 | 6.4:+3151862 | None:intergenic |
| TTTATCAATCAGGACTTGGA+AGG | 0.525684 | 6.4:-3146071 | MS.gene47310:intron |
| GTTTCTAGGTAAGGAATGGA+AGG | 0.528256 | 6.4:-3155133 | MS.gene47310:intron |
| TTATAGGATGTTGATGCTGA+GGG | 0.533321 | 6.4:-3150087 | MS.gene47310:intron |
| TCTGGAAGGAGCAAATTTGA+AGG | 0.533384 | 6.4:-3147811 | MS.gene47310:intron |
| TGGCGTGGTGCCCACTCTCG+AGG | 0.534975 | 6.4:-3151915 | MS.gene47310:CDS |
| AAGGTTAGCATTCTTCAACT+TGG | 0.536677 | 6.4:+3146935 | None:intergenic |
| TTTACTGGGGCAAATCTTCG+TGG | 0.542197 | 6.4:-3149947 | MS.gene47310:CDS |
| CCTGTAGATGTGCAGATCGC+AGG | 0.553123 | 6.4:+3149371 | None:intergenic |
| AGATTCGTGCATTGATTGGT+CGG | 0.553183 | 6.4:+3157666 | None:intergenic |
| GCTCATGTAATTGGGTCCTA+CGG | 0.573231 | 6.4:-3157628 | MS.gene47310:intron |
| TAATAAGAGGAAGGAAGAAG+AGG | 0.574612 | 6.4:-3151171 | MS.gene47310:CDS |
| TTACATGAGCGAATCGAAGT+CGG | 0.576324 | 6.4:+3157641 | None:intergenic |
| TTTATAGGATGTTGATGCTG+AGG | 0.585562 | 6.4:-3150088 | MS.gene47310:intron |
| ATTCTCAATTGGTTAAGGGA+TGG | 0.600914 | 6.4:-3151935 | MS.gene47310:CDS |
| ATATTCAGCTTCCTCGAGAG+TGG | 0.601502 | 6.4:+3151904 | None:intergenic |
| AGATGTCCTTAATAAGAGGA+AGG | 0.603876 | 6.4:-3151180 | MS.gene47310:CDS |
| AAGAATTTACCAGTTTAGAG+AGG | 0.607566 | 6.4:+3151057 | None:intergenic |
| CTTACTAATGCCAATCTGGA+GGG | 0.617585 | 6.4:-3147840 | MS.gene47310:CDS |
| TATTCAGCTTCCTCGAGAGT+GGG | 0.618214 | 6.4:+3151905 | None:intergenic |
| CTGGAGGGAGCTAATCTGGA+AGG | 0.618931 | 6.4:-3147825 | MS.gene47310:CDS |
| ATGCTCGCTGCTATGTTCAG+TGG | 0.625132 | 6.4:-3153714 | MS.gene47310:CDS |
| CAATTGGTTAAGGGATGGCG+TGG | 0.626299 | 6.4:-3151930 | MS.gene47310:CDS |
| TCTTACTAATGCCAATCTGG+AGG | 0.628999 | 6.4:-3147841 | MS.gene47310:CDS |
| CAGTACGTGTCAATTCTGTA+CGG | 0.635724 | 6.4:+3151144 | None:intergenic |
| TGAGAGAGTCAGGTTTCGAG+GGG | 0.661863 | 6.4:-3151099 | MS.gene47310:CDS |
| GGGAAGATGTGAATTTACTG+GGG | 0.736367 | 6.4:-3149960 | MS.gene47310:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 3146028 | 3157695 | 3146028 | ID=MS.gene47310 |
| chr6.4 | mRNA | 3146028 | 3157695 | 3146028 | ID=MS.gene47310.t1;Parent=MS.gene47310 |
| chr6.4 | exon | 3157629 | 3157695 | 3157629 | ID=MS.gene47310.t1.exon1;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3157629 | 3157695 | 3157629 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3155031 | 3155147 | 3155031 | ID=MS.gene47310.t1.exon2;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3155031 | 3155147 | 3155031 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3153683 | 3153792 | 3153683 | ID=MS.gene47310.t1.exon3;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3153683 | 3153792 | 3153683 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3151865 | 3151999 | 3151865 | ID=MS.gene47310.t1.exon4;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3151865 | 3151999 | 3151865 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3151067 | 3151222 | 3151067 | ID=MS.gene47310.t1.exon5;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3151067 | 3151222 | 3151067 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3150501 | 3150584 | 3150501 | ID=MS.gene47310.t1.exon6;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3150501 | 3150584 | 3150501 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3150057 | 3150103 | 3150057 | ID=MS.gene47310.t1.exon7;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3150057 | 3150103 | 3150057 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3149903 | 3149976 | 3149903 | ID=MS.gene47310.t1.exon8;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3149903 | 3149976 | 3149903 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3149372 | 3149430 | 3149372 | ID=MS.gene47310.t1.exon9;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3149372 | 3149430 | 3149372 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3147812 | 3147871 | 3147812 | ID=MS.gene47310.t1.exon10;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3147812 | 3147871 | 3147812 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3146896 | 3146976 | 3146896 | ID=MS.gene47310.t1.exon11;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3146896 | 3146976 | 3146896 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| chr6.4 | exon | 3146028 | 3146081 | 3146028 | ID=MS.gene47310.t1.exon12;Parent=MS.gene47310.t1 |
| chr6.4 | CDS | 3146028 | 3146081 | 3146028 | ID=cds.MS.gene47310.t1;Parent=MS.gene47310.t1 |
| Gene Sequence |
| Protein sequence |
