AlfalfaGEDB | MS.gene47943

M. sativa cultivar XinJiangDaYe / MS.gene47943

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47943.t1	GAU42749.1	41.8	141	81	1	7	146	279	419	8.20E-16	93.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47943.t1	A0A2Z6PDC9	41.8	141	81	1	7	146	279	419	5.9e-16	93.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049853	MS.gene47943	0.811532	6.68E-51	-1.69E-46
MS.gene052447	MS.gene47943	0.822896	1.85E-53	-1.69E-46
MS.gene054174	MS.gene47943	0.81914	1.36E-52	-1.69E-46
MS.gene054175	MS.gene47943	0.820644	6.15E-53	-1.69E-46
MS.gene054484	MS.gene47943	0.809595	1.75E-50	-1.69E-46
MS.gene058515	MS.gene47943	0.836503	9.01E-57	-1.69E-46
MS.gene059276	MS.gene47943	0.805165	1.52E-49	-1.69E-46
MS.gene059458	MS.gene47943	0.802296	6.00E-49	-1.69E-46
MS.gene059459	MS.gene47943	0.822791	1.96E-53	-1.69E-46
MS.gene059460	MS.gene47943	0.823366	1.44E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 33 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAATCACTTCATGCGATTC+TGG	0.165578	4.1:-11282810	None:intergenic
GGCCCAACTCCATCATTTCT+TGG	0.260591	4.1:-11282747	None:intergenic
AGAATCGCATGAAGTGATTA+AGG	0.283475	4.1:+11282812	MS.gene47943:CDS
TGCAGCAACACAAAGCAAAA+TGG	0.306707	4.1:+11282842	MS.gene47943:CDS
TAATCACTTCATGCGATTCT+GGG	0.385867	4.1:-11282809	None:intergenic
GGAATCTCAAACAAAACAAA+TGG	0.404559	4.1:+11283061	MS.gene47943:CDS
ATGTTAGTTTATAAGCATAT+TGG	0.408472	4.1:-11283147	None:intergenic
TGGATCAATTGAGTATCAAT+TGG	0.409277	4.1:-11282783	None:intergenic
CTTCATGCGATTCTGGGTTG+TGG	0.463420	4.1:-11282803	None:intergenic
GGCCAAGAAATGATGGAGTT+GGG	0.472970	4.1:+11282745	MS.gene47943:CDS
GCAGCAACACAAAGCAAAAT+GGG	0.473673	4.1:+11282843	MS.gene47943:CDS
AAAAGTGGGTGACAATATCA+TGG	0.475292	4.1:+11283109	MS.gene47943:CDS
TGACAATATCATGGGACTAA+AGG	0.486559	4.1:+11283118	MS.gene47943:CDS
CTAGAACTTCTCATCATGAA+AGG	0.498573	4.1:+11283015	MS.gene47943:CDS
GAATCGCATGAAGTGATTAA+GGG	0.500025	4.1:+11282813	MS.gene47943:CDS
TCAATTGGAACAGACTTACT+TGG	0.506371	4.1:-11282768	None:intergenic
AGGCCAAGAAATGATGGAGT+TGG	0.509705	4.1:+11282744	MS.gene47943:CDS
GAATCTCAAACAAAACAAAT+GGG	0.512097	4.1:+11283062	MS.gene47943:CDS
TAGAACTTCTCATCATGAAA+GGG	0.527774	4.1:+11283016	MS.gene47943:CDS
ACACAAAGCAAAATGGGTGG+TGG	0.528325	4.1:+11282849	MS.gene47943:CDS
CAAACAAAACAAATGGGAGA+TGG	0.532882	4.1:+11283068	MS.gene47943:CDS
ATTATGGACTCTTTCTCACT+AGG	0.556984	4.1:-11282915	None:intergenic
CACAAAGCAAAATGGGTGGT+GGG	0.566133	4.1:+11282850	MS.gene47943:CDS
AAGCAAAATGGGTGGTGGGA+AGG	0.579025	4.1:+11282854	MS.gene47943:CDS
AATCGCATGAAGTGATTAAG+GGG	0.587656	4.1:+11282814	MS.gene47943:CDS
AAAGTGGGTGACAATATCAT+GGG	0.614528	4.1:+11283110	MS.gene47943:CDS
CGCATGAAGTGATTAAGGGG+GGG	0.615999	4.1:+11282817	MS.gene47943:CDS
TGAAAGGGAAGTCGAATGCA+TGG	0.620914	4.1:+11283031	MS.gene47943:CDS
ACAAATGGGAGATGGAAGCA+TGG	0.631830	4.1:+11283076	MS.gene47943:CDS
AGTCGAATGCATGGTCAATG+TGG	0.653565	4.1:+11283040	MS.gene47943:CDS
TCGCATGAAGTGATTAAGGG+GGG	0.655623	4.1:+11282816	MS.gene47943:CDS
ATCGCATGAAGTGATTAAGG+GGG	0.672740	4.1:+11282815	MS.gene47943:CDS
GCAACACAAAGCAAAATGGG+TGG	0.687187	4.1:+11282846	MS.gene47943:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAATCTCAAACAAAACAAAT+GGG	+	chr4.1:11283062-11283081	MS.gene47943:CDS	25.0%
!	GCTTGAAAAACAAGAAATAA+TGG	+	chr4.1:11282938-11282957	MS.gene47943:CDS	25.0%
!!!	TTCTTGTTTTTCAAGCATTA+TGG	-	chr4.1:11282934-11282953	None:intergenic	25.0%
	AAAAAAAGGCCAAGAAATGA+TGG	+	chr4.1:11282738-11282757	MS.gene47943:CDS	30.0%
	GGAATCTCAAACAAAACAAA+TGG	+	chr4.1:11283061-11283080	MS.gene47943:CDS	30.0%
	TAGAACTTCTCATCATGAAA+GGG	+	chr4.1:11283016-11283035	MS.gene47943:CDS	30.0%
	TGGATCAATTGAGTATCAAT+TGG	-	chr4.1:11282786-11282805	None:intergenic	30.0%
	AAAAGTGGGTGACAATATCA+TGG	+	chr4.1:11283109-11283128	MS.gene47943:CDS	35.0%
	AAAGTGGGTGACAATATCAT+GGG	+	chr4.1:11283110-11283129	MS.gene47943:CDS	35.0%
	AATCGCATGAAGTGATTAAG+GGG	+	chr4.1:11282814-11282833	MS.gene47943:CDS	35.0%
	AGAATCGCATGAAGTGATTA+AGG	+	chr4.1:11282812-11282831	MS.gene47943:CDS	35.0%
	ATTATGGACTCTTTCTCACT+AGG	-	chr4.1:11282918-11282937	None:intergenic	35.0%
	CAAACAAAACAAATGGGAGA+TGG	+	chr4.1:11283068-11283087	MS.gene47943:CDS	35.0%
	CTAGAACTTCTCATCATGAA+AGG	+	chr4.1:11283015-11283034	MS.gene47943:CDS	35.0%
	GAATCGCATGAAGTGATTAA+GGG	+	chr4.1:11282813-11282832	MS.gene47943:CDS	35.0%
	TAATCACTTCATGCGATTCT+GGG	-	chr4.1:11282812-11282831	None:intergenic	35.0%
	TCAATTGGAACAGACTTACT+TGG	-	chr4.1:11282771-11282790	None:intergenic	35.0%
	TGACAATATCATGGGACTAA+AGG	+	chr4.1:11283118-11283137	MS.gene47943:CDS	35.0%
	TTAATCACTTCATGCGATTC+TGG	-	chr4.1:11282813-11282832	None:intergenic	35.0%
	ATCGCATGAAGTGATTAAGG+GGG	+	chr4.1:11282815-11282834	MS.gene47943:CDS	40.0%
	ATGGACGTGACTCAAAAAGT+GGG	+	chr4.1:11283095-11283114	MS.gene47943:CDS	40.0%
	GCAGCAACACAAAGCAAAAT+GGG	+	chr4.1:11282843-11282862	MS.gene47943:CDS	40.0%
	TGCAGCAACACAAAGCAAAA+TGG	+	chr4.1:11282842-11282861	MS.gene47943:CDS	40.0%
	ACAAATGGGAGATGGAAGCA+TGG	+	chr4.1:11283076-11283095	MS.gene47943:CDS	45.0%
	ACACAAAGCAAAATGGGTGG+TGG	+	chr4.1:11282849-11282868	MS.gene47943:CDS	45.0%
	AGTCGAATGCATGGTCAATG+TGG	+	chr4.1:11283040-11283059	MS.gene47943:CDS	45.0%
	CACAAAGCAAAATGGGTGGT+GGG	+	chr4.1:11282850-11282869	MS.gene47943:CDS	45.0%
	CATGGACGTGACTCAAAAAG+TGG	+	chr4.1:11283094-11283113	MS.gene47943:CDS	45.0%
	GCAACACAAAGCAAAATGGG+TGG	+	chr4.1:11282846-11282865	MS.gene47943:CDS	45.0%
	TCGCATGAAGTGATTAAGGG+GGG	+	chr4.1:11282816-11282835	MS.gene47943:CDS	45.0%
	TGAAAGGGAAGTCGAATGCA+TGG	+	chr4.1:11283031-11283050	MS.gene47943:CDS	45.0%
!	AGGCCAAGAAATGATGGAGT+TGG	+	chr4.1:11282744-11282763	MS.gene47943:CDS	45.0%
!	GGCCAAGAAATGATGGAGTT+GGG	+	chr4.1:11282745-11282764	MS.gene47943:CDS	45.0%
	CGCATGAAGTGATTAAGGGG+GGG	+	chr4.1:11282817-11282836	MS.gene47943:CDS	50.0%
	CTTCATGCGATTCTGGGTTG+TGG	-	chr4.1:11282806-11282825	None:intergenic	50.0%
	GGCCCAACTCCATCATTTCT+TGG	-	chr4.1:11282750-11282769	None:intergenic	50.0%
!!	AAGCAAAATGGGTGGTGGGA+AGG	+	chr4.1:11282854-11282873	MS.gene47943:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	11282726	11283166	11282726	ID=MS.gene47943
chr4.1	mRNA	11282726	11283166	11282726	ID=MS.gene47943.t1;Parent=MS.gene47943
chr4.1	exon	11282726	11283166	11282726	ID=MS.gene47943.t1.exon1;Parent=MS.gene47943.t1
chr4.1	CDS	11282726	11283166	11282726	ID=cds.MS.gene47943.t1;Parent=MS.gene47943.t1

Gene Sequence

>MS.gene47943

ATGTGGTTTAAGAAAAAAAGGCCAAGAAATGATGGAGTTGGGCCAAGTAAGTCTGTTCCAATTGATACTCAATTGATCCACAACCCAGAATCGCATGAAGTGATTAAGGGGGGGTATGCAGCAACACAAAGCAAAATGGGTGGTGGGAAGGCTCAAGTGCAGCAGTACAAGTCTAAAGAAAATCGAGTTCCTAGTGAGAAAGAGTCCATAATGCTTGAAAAACAAGAAATAATGGTCTCGACACACAAGCATGAGCAACACACAGCAATATTACATCACGATAACTCAACTAGAACTTCTCATCATGAAAGGGAAGTCGAATGCATGGTCAATGTGGAATCTCAAACAAAACAAATGGGAGATGGAAGCATGGACGTGACTCAAAAAGTGGGTGACAATATCATGGGACTAAAGGATGACACCAATATGCTTATAAACTAA

Protein sequence

>MS.gene47943.t1

MWFKKKRPRNDGVGPSKSVPIDTQLIHNPESHEVIKGGYAATQSKMGGGKAQVQQYKSKENRVPSEKESIMLEKQEIMVSTHKHEQHTAILHHDNSTRTSHHEREVECMVNVESQTKQMGDGSMDVTQKVGDNIMGLKDDTNMLIN