AlfalfaGEDB | MS.gene48044

M. sativa cultivar XinJiangDaYe / MS.gene48044

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48044.t1	RHN57662.1	97.2	144	4	0	1	144	51	194	2.80E-77	297.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48044.t1	G7KD26	97.2	144	4	0	1	144	31	174	2.0e-77	297.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050060	MS.gene48044	0.822114	2.82E-53	-1.69E-46
MS.gene050426	MS.gene48044	-0.80397	2.70E-49	-1.69E-46
MS.gene050971	MS.gene48044	0.8024	5.71E-49	-1.69E-46
MS.gene052188	MS.gene48044	0.802054	6.73E-49	-1.69E-46
MS.gene052340	MS.gene48044	0.829334	5.45E-55	-1.69E-46
MS.gene052469	MS.gene48044	0.82016	7.94E-53	-1.69E-46
MS.gene054327	MS.gene48044	0.803734	3.03E-49	-1.69E-46
MS.gene05553	MS.gene48044	0.819823	9.49E-53	-1.69E-46
MS.gene05556	MS.gene48044	0.834974	2.20E-56	-1.69E-46
MS.gene061159	MS.gene48044	0.806354	8.57E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48044.t1	MTR_5g091100	97.222	144	4	0	1	144	31	174	8.43e-101	286

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGACTTTGGTTATGTTAA+TGG	0.196023	5.2:+77077120	MS.gene48044:CDS
TGCTCCTCGTCCATCATGTT+AGG	0.275785	5.2:-77076943	None:intergenic
GGGAGGTTAGACTTTGACTT+TGG	0.409070	5.2:+77077108	MS.gene48044:CDS
ACGACATGGTGACAATGAAT+TGG	0.423515	5.2:+77077070	MS.gene48044:CDS
GAGTTTAGTAGATATTGATT+TGG	0.428299	5.2:+77076810	MS.gene48044:CDS
GGGGCAGAAACACAACTGTT+TGG	0.454871	5.2:+77076969	MS.gene48044:CDS
ATGTCGTCTAGTGGGTTGGC+TGG	0.479458	5.2:-77077054	None:intergenic
GCTCCTCGTCCATCATGTTA+GGG	0.479505	5.2:-77076942	None:intergenic
TTTAGTAGATATTGATTTGG+AGG	0.482961	5.2:+77076813	MS.gene48044:CDS
AGAACTGCTCGAAGAGGAAG+AGG	0.487533	5.2:+77076864	MS.gene48044:CDS
TGCAAACTACGGTTGCTTGT+GGG	0.509498	5.2:+77077185	MS.gene48044:CDS
ATTGTCACCATGTCGTCTAG+TGG	0.522929	5.2:-77077063	None:intergenic
TAATGTTAACCCTAACATGA+TGG	0.537310	5.2:+77076933	MS.gene48044:CDS
AAATGTCCATCGCAAATTTG+AGG	0.538505	5.2:-77077153	None:intergenic
CTTTGAGTGGCTCAATATGA+TGG	0.540525	5.2:+77077020	MS.gene48044:CDS
TTCTCTAATGATGCAAACTA+CGG	0.542851	5.2:+77077174	MS.gene48044:CDS
CACCATGTCGTCTAGTGGGT+TGG	0.549004	5.2:-77077058	None:intergenic
ACTATGTTCATGACTTTGAG+TGG	0.550991	5.2:+77077007	MS.gene48044:CDS
TTGTCACCATGTCGTCTAGT+GGG	0.562995	5.2:-77077062	None:intergenic
CATGATGGACGAGGAGCAAG+AGG	0.574215	5.2:+77076948	MS.gene48044:CDS
GCAAACTACGGTTGCTTGTG+GGG	0.605837	5.2:+77077186	MS.gene48044:CDS
CTTGAGAGAACTGCTCGAAG+AGG	0.606445	5.2:+77076858	MS.gene48044:CDS
ATGCAAACTACGGTTGCTTG+TGG	0.608638	5.2:+77077184	MS.gene48044:CDS
GCTCAATATGATGGACATGA+TGG	0.611078	5.2:+77077029	MS.gene48044:CDS
CAACTGTTTGGAAAAGAGTG+AGG	0.629243	5.2:+77076981	MS.gene48044:CDS
TGATGGACGAGGAGCAAGAG+GGG	0.644482	5.2:+77076950	MS.gene48044:CDS
AACTGTTTGGAAAAGAGTGA+GGG	0.651646	5.2:+77076982	MS.gene48044:CDS
AGCCAACCCACTAGACGACA+TGG	0.657824	5.2:+77077056	MS.gene48044:CDS
TAACCCTAACATGATGGACG+AGG	0.680759	5.2:+77076939	MS.gene48044:CDS
ATGATGGACGAGGAGCAAGA+GGG	0.691206	5.2:+77076949	MS.gene48044:CDS
GAACTGCTCGAAGAGGAAGA+GGG	0.710481	5.2:+77076865	MS.gene48044:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAGTTTAGTAGATATTGATT+TGG	+	chr5.2:77076810-77076829	MS.gene48044:CDS	25.0%
!!	TTTGACTTTGGTTATGTTAA+TGG	+	chr5.2:77077120-77077139	MS.gene48044:CDS	25.0%
!!!	TTTAGTAGATATTGATTTGG+AGG	+	chr5.2:77076813-77076832	MS.gene48044:CDS	25.0%
	TAATGTTAACCCTAACATGA+TGG	+	chr5.2:77076933-77076952	MS.gene48044:CDS	30.0%
	TTCTCTAATGATGCAAACTA+CGG	+	chr5.2:77077174-77077193	MS.gene48044:CDS	30.0%
!	CGTATCATTATTTTCACATG+AGG	-	chr5.2:77076791-77076810	None:intergenic	30.0%
!!!	AATTGGTTTTCAGATGATGT+TGG	+	chr5.2:77077087-77077106	MS.gene48044:CDS	30.0%
!!!	ATTGGTTTTCAGATGATGTT+GGG	+	chr5.2:77077088-77077107	MS.gene48044:CDS	30.0%
	AAATGTCCATCGCAAATTTG+AGG	-	chr5.2:77077156-77077175	None:intergenic	35.0%
	AACTGTTTGGAAAAGAGTGA+GGG	+	chr5.2:77076982-77077001	MS.gene48044:CDS	35.0%
	ACTATGTTCATGACTTTGAG+TGG	+	chr5.2:77077007-77077026	MS.gene48044:CDS	35.0%
!	TTTTGTCCTCAAATTTGCGA+TGG	+	chr5.2:77077147-77077166	MS.gene48044:CDS	35.0%
	ACGACATGGTGACAATGAAT+TGG	+	chr5.2:77077070-77077089	MS.gene48044:CDS	40.0%
	CAACTGTTTGGAAAAGAGTG+AGG	+	chr5.2:77076981-77077000	MS.gene48044:CDS	40.0%
	CTTTGAGTGGCTCAATATGA+TGG	+	chr5.2:77077020-77077039	MS.gene48044:CDS	40.0%
!!	GCTCAATATGATGGACATGA+TGG	+	chr5.2:77077029-77077048	MS.gene48044:CDS	40.0%
	ATGCAAACTACGGTTGCTTG+TGG	+	chr5.2:77077184-77077203	MS.gene48044:CDS	45.0%
	ATTGTCACCATGTCGTCTAG+TGG	-	chr5.2:77077066-77077085	None:intergenic	45.0%
	TGCAAACTACGGTTGCTTGT+GGG	+	chr5.2:77077185-77077204	MS.gene48044:CDS	45.0%
	TTGTCACCATGTCGTCTAGT+GGG	-	chr5.2:77077065-77077084	None:intergenic	45.0%
!	GGGAGGTTAGACTTTGACTT+TGG	+	chr5.2:77077108-77077127	MS.gene48044:CDS	45.0%
!	GGTTTTCAGATGATGTTGGG+AGG	+	chr5.2:77077091-77077110	MS.gene48044:CDS	45.0%
!!	TAACCCTAACATGATGGACG+AGG	+	chr5.2:77076939-77076958	MS.gene48044:CDS	45.0%
	AGAACTGCTCGAAGAGGAAG+AGG	+	chr5.2:77076864-77076883	MS.gene48044:CDS	50.0%
	CTTGAGAGAACTGCTCGAAG+AGG	+	chr5.2:77076858-77076877	MS.gene48044:CDS	50.0%
	GAACTGCTCGAAGAGGAAGA+GGG	+	chr5.2:77076865-77076884	MS.gene48044:CDS	50.0%
	GCAAACTACGGTTGCTTGTG+GGG	+	chr5.2:77077186-77077205	MS.gene48044:CDS	50.0%
	GCTCCTCGTCCATCATGTTA+GGG	-	chr5.2:77076945-77076964	None:intergenic	50.0%
	GGGGCAGAAACACAACTGTT+TGG	+	chr5.2:77076969-77076988	MS.gene48044:CDS	50.0%
	TGCTCCTCGTCCATCATGTT+AGG	-	chr5.2:77076946-77076965	None:intergenic	50.0%
!!	ATGATGGACGAGGAGCAAGA+GGG	+	chr5.2:77076949-77076968	MS.gene48044:CDS	50.0%
	AGCCAACCCACTAGACGACA+TGG	+	chr5.2:77077056-77077075	MS.gene48044:CDS	55.0%
!	CACCATGTCGTCTAGTGGGT+TGG	-	chr5.2:77077061-77077080	None:intergenic	55.0%
!	TGATGGACGAGGAGCAAGAG+GGG	+	chr5.2:77076950-77076969	MS.gene48044:CDS	55.0%
!!	ATGTCGTCTAGTGGGTTGGC+TGG	-	chr5.2:77077057-77077076	None:intergenic	55.0%
!!	CATGATGGACGAGGAGCAAG+AGG	+	chr5.2:77076948-77076967	MS.gene48044:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	77076784	77077218	77076784	ID=MS.gene48044
chr5.2	mRNA	77076784	77077218	77076784	ID=MS.gene48044.t1;Parent=MS.gene48044
chr5.2	exon	77076784	77077218	77076784	ID=MS.gene48044.t1.exon1;Parent=MS.gene48044.t1
chr5.2	CDS	77076784	77077218	77076784	ID=cds.MS.gene48044.t1;Parent=MS.gene48044.t1

Gene Sequence

>MS.gene48044

ATGGCCTCATGTGAAAATAATGATACGAGTTTAGTAGATATTGATTTGGAGGCATTTGAAATTGATGAAAATCTCTTGAGAGAACTGCTCGAAGAGGAAGAGGGCAAGAGTGATGTTCATGATAACAAAGAATGCATAGAAGAAAGCATTAATGTTAACCCTAACATGATGGACGAGGAGCAAGAGGGGCAGAAACACAACTGTTTGGAAAAGAGTGAGGGTCACTATGTTCATGACTTTGAGTGGCTCAATATGATGGACATGATGGAGCCAGCCAACCCACTAGACGACATGGTGACAATGAATTGGTTTTCAGATGATGTTGGGAGGTTAGACTTTGACTTTGGTTATGTTAATGGAGAATTTTGTCCTCAAATTTGCGATGGACATTTCTCTAATGATGCAAACTACGGTTGCTTGTGGGGAGAATATTGA

Protein sequence

>MS.gene48044.t1

MASCENNDTSLVDIDLEAFEIDENLLRELLEEEEGKSDVHDNKECIEESINVNPNMMDEEQEGQKHNCLEKSEGHYVHDFEWLNMMDMMEPANPLDDMVTMNWFSDDVGRLDFDFGYVNGEFCPQICDGHFSNDANYGCLWGEY