Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48240.t1 | XP_024638078.1 | 98.2 | 397 | 7 | 0 | 1 | 397 | 1 | 397 | 2.20E-236 | 827.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48240.t1 | Q9SLN5 | 82.1 | 396 | 70 | 1 | 1 | 395 | 1 | 396 | 1.1e-201 | 703.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48240.t1 | A0A396I104 | 98.2 | 397 | 7 | 0 | 1 | 397 | 1 | 397 | 1.6e-236 | 827.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene40160 | MS.gene48240 | PPI |
| MS.gene93798 | MS.gene48240 | PPI |
| MS.gene48240 | MS.gene58403 | PPI |
| MS.gene033096 | MS.gene48240 | PPI |
| MS.gene064281 | MS.gene48240 | PPI |
| MS.gene044411 | MS.gene48240 | PPI |
| MS.gene48240 | MS.gene93411 | PPI |
| MS.gene91353 | MS.gene48240 | PPI |
| MS.gene48240 | MS.gene028573 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48240.t1 | MTR_5g017630 | 48.763 | 283 | 139 | 2 | 100 | 382 | 73 | 349 | 5.83e-90 | 275 |
| MS.gene48240.t1 | MTR_5g053940 | 46.996 | 283 | 144 | 2 | 100 | 382 | 78 | 354 | 1.94e-85 | 264 |
| MS.gene48240.t1 | MTR_5g053940 | 46.996 | 283 | 144 | 2 | 100 | 382 | 54 | 330 | 2.53e-85 | 263 |
| MS.gene48240.t1 | MTR_5g017630 | 48.101 | 237 | 117 | 2 | 100 | 336 | 73 | 303 | 2.33e-70 | 224 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48240.t1 | AT2G45240 | 81.864 | 397 | 71 | 1 | 1 | 396 | 1 | 397 | 0.0 | 696 |
| MS.gene48240.t1 | AT2G45240 | 79.751 | 321 | 64 | 1 | 1 | 320 | 1 | 321 | 0.0 | 552 |
| MS.gene48240.t1 | AT1G13270 | 48.561 | 278 | 137 | 2 | 105 | 382 | 96 | 367 | 6.90e-92 | 281 |
| MS.gene48240.t1 | AT4G37040 | 49.470 | 283 | 137 | 3 | 100 | 382 | 72 | 348 | 7.60e-89 | 273 |
| MS.gene48240.t1 | AT3G25740 | 44.840 | 281 | 144 | 4 | 105 | 382 | 69 | 341 | 5.12e-80 | 250 |
| MS.gene48240.t1 | AT1G13270 | 44.560 | 193 | 102 | 1 | 105 | 297 | 96 | 283 | 6.16e-54 | 180 |
| MS.gene48240.t1 | AT3G25740 | 42.500 | 120 | 60 | 2 | 105 | 222 | 69 | 181 | 1.77e-22 | 95.9 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTACGTGCAGCAAGATTT+TGG | 0.169908 | 4.2:-77522467 | None:intergenic |
| AGCTTCAACTCCATGCATTT+TGG | 0.175614 | 4.2:-77519325 | None:intergenic |
| CCCGTAAAGGTTCAGCATTC+TGG | 0.227224 | 4.2:+77519350 | MS.gene48240:CDS |
| GCACCAAACATTCCTCATTA+TGG | 0.269416 | 4.2:+77524067 | MS.gene48240:CDS |
| ATAACTATTGGGCTCATCTT+TGG | 0.289295 | 4.2:-77521405 | None:intergenic |
| CAAAGCTTCCTTACTTTGAT+TGG | 0.291250 | 4.2:+77520903 | MS.gene48240:CDS |
| TCAATTTGAGCATACTCTTT+TGG | 0.293472 | 4.2:+77524658 | MS.gene48240:CDS |
| AATACATACTGAAGATTTGC+AGG | 0.300572 | 4.2:-77518561 | None:intergenic |
| GACAATCCGTTTACCAGATA+TGG | 0.303126 | 4.2:-77521187 | None:intergenic |
| ACCAGAATGCTGAACCTTTA+CGG | 0.306863 | 4.2:-77519351 | None:intergenic |
| CCAGAATGCTGAACCTTTAC+GGG | 0.333069 | 4.2:-77519350 | None:intergenic |
| CTCATTCTTTCGATCTGGTC+TGG | 0.334220 | 4.2:-77521557 | None:intergenic |
| TCATATTGTGGTCATGGAAT+TGG | 0.341090 | 4.2:+77524028 | MS.gene48240:CDS |
| CATATACCTGCATTGATCAT+TGG | 0.342192 | 4.2:-77524498 | None:intergenic |
| CATAATCAGGTGACAGAAAC+TGG | 0.345296 | 4.2:+77526077 | MS.gene48240:intron |
| TAACTATTGGGCTCATCTTT+GGG | 0.350866 | 4.2:-77521404 | None:intergenic |
| CTTCCATAATGAGGAATGTT+TGG | 0.353164 | 4.2:-77524070 | None:intergenic |
| AGTATGCTGAAGATAACTAT+TGG | 0.372630 | 4.2:-77521417 | None:intergenic |
| CGTTTACCAGATATGGGATA+TGG | 0.372750 | 4.2:-77521180 | None:intergenic |
| TTTCTCTCATTCTTTCGATC+TGG | 0.374646 | 4.2:-77521562 | None:intergenic |
| CTCATATTGCTATACCTGAT+TGG | 0.383744 | 4.2:+77521214 | MS.gene48240:CDS |
| TGATAACTTCACCGATTTCA+CGG | 0.389125 | 4.2:-77523848 | None:intergenic |
| ATACTGAAGATTTGCAGGTT+TGG | 0.398456 | 4.2:-77518556 | None:intergenic |
| AGCACAAGAGAGTTTATTAA+CGG | 0.409837 | 4.2:-77518526 | None:intergenic |
| GTGTACTATAAAGGTGTTCA+TGG | 0.410468 | 4.2:+77523044 | MS.gene48240:CDS |
| CTTCCTTACTTTGATTGGAC+TGG | 0.410698 | 4.2:+77520908 | MS.gene48240:CDS |
| CAATCAAAGTAAGGAAGCTT+TGG | 0.411005 | 4.2:-77520902 | None:intergenic |
| GTGCGGCCATATCCCATATC+TGG | 0.431336 | 4.2:+77521174 | MS.gene48240:CDS |
| CCATCGAATTCAGTTAAACC+TGG | 0.435170 | 4.2:+77523816 | MS.gene48240:intron |
| GTATGCTGAAGATAACTATT+GGG | 0.447741 | 4.2:-77521416 | None:intergenic |
| CACATATGAGTGCTTGGATA+AGG | 0.447878 | 4.2:+77523210 | MS.gene48240:CDS |
| CTCACATCATCAGCCCAATC+AGG | 0.450396 | 4.2:-77521228 | None:intergenic |
| TTTGGAGTTCGACCCTGCCC+TGG | 0.451577 | 4.2:-77520884 | None:intergenic |
| AATCGTCATGCTTCAATGTC+AGG | 0.452960 | 4.2:+77523870 | MS.gene48240:CDS |
| TCATATTGCTATACCTGATT+GGG | 0.457094 | 4.2:+77521215 | MS.gene48240:CDS |
| TGGTTATATTGCGTGAAGCC+AGG | 0.459022 | 4.2:+77520866 | MS.gene48240:CDS |
| CATATCCCATATCTGGTAAA+CGG | 0.459117 | 4.2:+77521181 | MS.gene48240:CDS |
| TTCCTTACTTTGATTGGACT+GGG | 0.461313 | 4.2:+77520909 | MS.gene48240:CDS |
| GGGGTACGGTTCCGTGAAAT+CGG | 0.473006 | 4.2:+77523837 | MS.gene48240:CDS |
| TCTCAATGATACATACTTTG+TGG | 0.473493 | 4.2:+77523150 | MS.gene48240:CDS |
| CATCACCGAATGTTTATCCT+TGG | 0.478072 | 4.2:+77526129 | MS.gene48240:CDS |
| TTTAACCAAGGATAAACATT+CGG | 0.478433 | 4.2:-77526134 | None:intergenic |
| CTCAATGATACATACTTTGT+GGG | 0.479150 | 4.2:+77523151 | MS.gene48240:CDS |
| TACCCAGTCCAATCAAAGTA+AGG | 0.487570 | 4.2:-77520911 | None:intergenic |
| GTAAAATCATATTGTGGTCA+TGG | 0.489624 | 4.2:+77524022 | MS.gene48240:CDS |
| ACAATCCGTTTACCAGATAT+GGG | 0.495773 | 4.2:-77521186 | None:intergenic |
| AAATGGTAATTGAGAGTCGA+TGG | 0.498268 | 4.2:-77522440 | None:intergenic |
| GTAGATTGGAAGGGAGGTGT+TGG | 0.501126 | 4.2:+77522150 | MS.gene48240:intron |
| GATCATTGGCTCAATTGTAA+AGG | 0.502055 | 4.2:-77524484 | None:intergenic |
| AGATTGATCTACCTTGCATC+AGG | 0.507996 | 4.2:-77522599 | None:intergenic |
| TCAGGTATAGCAATATGAGC+TGG | 0.510937 | 4.2:-77521210 | None:intergenic |
| CACGAGGCAACTATTGCTGC+AGG | 0.513450 | 4.2:+77522232 | MS.gene48240:CDS |
| CTGGCGTGATCGACTGTGGC+CGG | 0.519748 | 4.2:+77524595 | MS.gene48240:CDS |
| GTTAATGAAGTTATCTGCCA+TGG | 0.522636 | 4.2:+77522572 | MS.gene48240:CDS |
| GATGTTACTGTGTACTATAA+AGG | 0.523128 | 4.2:+77523035 | MS.gene48240:CDS |
| ATATTGCGTGAAGCCAGGGC+AGG | 0.525994 | 4.2:+77520871 | MS.gene48240:CDS |
| CAGACTCACTTGGTGAAGGA+TGG | 0.527445 | 4.2:+77520846 | MS.gene48240:CDS |
| CATCGAATTCAGTTAAACCT+GGG | 0.533432 | 4.2:+77523817 | MS.gene48240:intron |
| CCCAATTATGAATTTAACCA+AGG | 0.537081 | 4.2:-77526146 | None:intergenic |
| ATCGACTGTGGCCGGATGGA+TGG | 0.541522 | 4.2:+77524603 | MS.gene48240:CDS |
| AAGAATGAGAGAAACATGTC+GGG | 0.542497 | 4.2:+77521571 | MS.gene48240:CDS |
| TGATTCGTAGATTGGAAGGG+AGG | 0.546142 | 4.2:+77522144 | MS.gene48240:intron |
| GCCATGGAATTCCTGATGCA+AGG | 0.548278 | 4.2:+77522588 | MS.gene48240:CDS |
| GCCGCTCGGATTATTCAGCC+TGG | 0.550022 | 4.2:+77522178 | MS.gene48240:CDS |
| ATTCAGTTAAACCTGGGGTA+CGG | 0.555886 | 4.2:+77523823 | MS.gene48240:intron |
| TCTCAGGTGTCCAAAATGCA+TGG | 0.557907 | 4.2:+77519315 | MS.gene48240:intron |
| CAAATGCACATATGAGTGCT+TGG | 0.558213 | 4.2:+77523204 | MS.gene48240:CDS |
| AAATTGATTCGTAGATTGGA+AGG | 0.558774 | 4.2:+77522140 | MS.gene48240:intron |
| AGGTGTTGGATGCAGCCGCT+CGG | 0.560148 | 4.2:+77522164 | MS.gene48240:CDS |
| GGTTATATTGCGTGAAGCCA+GGG | 0.560500 | 4.2:+77520867 | MS.gene48240:CDS |
| GTCATTCTACAGGAAGCTTG+AGG | 0.564322 | 4.2:+77522992 | MS.gene48240:intron |
| TAAAGCAGTTGGTGTGATGA+AGG | 0.565699 | 4.2:+77524452 | MS.gene48240:CDS |
| ATTGAGCCAATGATCAATGC+AGG | 0.566962 | 4.2:+77524492 | MS.gene48240:CDS |
| ATTTCACGGAACCGTACCCC+AGG | 0.568344 | 4.2:-77523834 | None:intergenic |
| GGATAAACATTCGGTGATGT+CGG | 0.569817 | 4.2:-77526125 | None:intergenic |
| TTGGATAAGGCAATATCCAT+TGG | 0.569824 | 4.2:+77523223 | MS.gene48240:CDS |
| GTAACATCAGTCCATCCATC+CGG | 0.570192 | 4.2:-77524614 | None:intergenic |
| CAGGTATAGCAATATGAGCT+GGG | 0.571387 | 4.2:-77521209 | None:intergenic |
| CAAAGGATACTTCCATAATG+AGG | 0.573541 | 4.2:-77524079 | None:intergenic |
| GAGTTGAAGCTTCCCCGTAA+AGG | 0.576539 | 4.2:+77519337 | MS.gene48240:CDS |
| AAGGCAAAGGTATCCTCAGT+TGG | 0.584987 | 4.2:+77520812 | MS.gene48240:CDS |
| CGTGATCGACTGTGGCCGGA+TGG | 0.586768 | 4.2:+77524599 | MS.gene48240:CDS |
| ATTTCATCAGTTGTCACACC+AGG | 0.591424 | 4.2:-77522196 | None:intergenic |
| TTCTACAGGAAGCTTGAGGA+TGG | 0.596903 | 4.2:+77522996 | MS.gene48240:intron |
| GCAGTTGGTGTGATGAAGGC+TGG | 0.599210 | 4.2:+77524456 | MS.gene48240:CDS |
| TTATCTTCAGCATACTGTTG+AGG | 0.599339 | 4.2:+77521424 | MS.gene48240:CDS |
| AAAGAATGAGAGAAACATGT+CGG | 0.599956 | 4.2:+77521570 | MS.gene48240:CDS |
| ATCAGTGCATCTGAAGGCAA+AGG | 0.606762 | 4.2:+77520799 | MS.gene48240:CDS |
| TACATACTTTGTGGGAAATG+TGG | 0.607592 | 4.2:+77523159 | MS.gene48240:CDS |
| GCACAAATCAGTGCATCTGA+AGG | 0.608305 | 4.2:+77520793 | MS.gene48240:CDS |
| TGGACTGATGTTACAGCAGA+TGG | 0.608343 | 4.2:+77524623 | MS.gene48240:CDS |
| AATGGTAATTGAGAGTCGAT+GGG | 0.615386 | 4.2:-77522439 | None:intergenic |
| GGATGAAGAATCTCGTCAGT+TGG | 0.618217 | 4.2:+77523180 | MS.gene48240:CDS |
| CTGGCAGGAAATAAAGCAGT+TGG | 0.619503 | 4.2:+77524441 | MS.gene48240:intron |
| ACACAGAATTCAGACTCACT+TGG | 0.624277 | 4.2:+77520836 | MS.gene48240:CDS |
| TATTGCGTGAAGCCAGGGCA+GGG | 0.629603 | 4.2:+77520872 | MS.gene48240:CDS |
| AATTGATTCGTAGATTGGAA+GGG | 0.630358 | 4.2:+77522141 | MS.gene48240:intron |
| AATTCAGACTCACTTGGTGA+AGG | 0.644701 | 4.2:+77520842 | MS.gene48240:CDS |
| ACCTTGCATCAGGAATTCCA+TGG | 0.647124 | 4.2:-77522589 | None:intergenic |
| GAGGAATGTTTGGTGCACAA+TGG | 0.651672 | 4.2:-77524060 | None:intergenic |
| TTACAGGTAAAATCATATTG+TGG | 0.652206 | 4.2:+77524016 | MS.gene48240:intron |
| ATCGAATTCAGTTAAACCTG+GGG | 0.659350 | 4.2:+77523818 | MS.gene48240:intron |
| TGAATTCTGTGTGCCAACTG+AGG | 0.670477 | 4.2:-77520825 | None:intergenic |
| GTGTCTGGCGTGATCGACTG+TGG | 0.700621 | 4.2:+77524591 | MS.gene48240:CDS |
| CTGCTCTTGCAAGGGCAGTG+CGG | 0.706767 | 4.2:+77521157 | MS.gene48240:intron |
| CAGAATGCTGAACCTTTACG+GGG | 0.709765 | 4.2:-77519349 | None:intergenic |
| TTCAATGTCAGGCTTCTCAG+TGG | 0.712844 | 4.2:+77523881 | MS.gene48240:CDS |
| ACCAGGCTGAATAATCCGAG+CGG | 0.746152 | 4.2:-77522179 | None:intergenic |
| AATTGACAGAGTAGTTCACG+AGG | 0.767513 | 4.2:+77522216 | MS.gene48240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 77518504 | 77526163 | 77518504 | ID=MS.gene48240 |
| chr4.2 | mRNA | 77518504 | 77526163 | 77518504 | ID=MS.gene48240.t1;Parent=MS.gene48240 |
| chr4.2 | exon | 77518504 | 77518574 | 77518504 | ID=MS.gene48240.t1.exon1;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77518504 | 77518574 | 77518504 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77519321 | 77519371 | 77519321 | ID=MS.gene48240.t1.exon2;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77519321 | 77519371 | 77519321 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77520760 | 77520930 | 77520760 | ID=MS.gene48240.t1.exon3;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77520760 | 77520930 | 77520760 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77521170 | 77521245 | 77521170 | ID=MS.gene48240.t1.exon4;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77521170 | 77521245 | 77521170 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77521398 | 77521445 | 77521398 | ID=MS.gene48240.t1.exon5;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77521398 | 77521445 | 77521398 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77521548 | 77521592 | 77521548 | ID=MS.gene48240.t1.exon6;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77521548 | 77521592 | 77521548 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77522154 | 77522253 | 77522154 | ID=MS.gene48240.t1.exon7;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77522154 | 77522253 | 77522154 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77522432 | 77522483 | 77522432 | ID=MS.gene48240.t1.exon8;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77522432 | 77522483 | 77522432 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77522568 | 77522609 | 77522568 | ID=MS.gene48240.t1.exon9;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77522568 | 77522609 | 77522568 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77523004 | 77523065 | 77523004 | ID=MS.gene48240.t1.exon10;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77523004 | 77523065 | 77523004 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77523146 | 77523244 | 77523146 | ID=MS.gene48240.t1.exon11;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77523146 | 77523244 | 77523146 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77523829 | 77523902 | 77523829 | ID=MS.gene48240.t1.exon12;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77523829 | 77523902 | 77523829 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77524022 | 77524091 | 77524022 | ID=MS.gene48240.t1.exon13;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77524022 | 77524091 | 77524022 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77524448 | 77524513 | 77524448 | ID=MS.gene48240.t1.exon14;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77524448 | 77524513 | 77524448 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77524591 | 77524679 | 77524591 | ID=MS.gene48240.t1.exon15;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77524591 | 77524679 | 77524591 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| chr4.2 | exon | 77526086 | 77526163 | 77526086 | ID=MS.gene48240.t1.exon16;Parent=MS.gene48240.t1 |
| chr4.2 | CDS | 77526086 | 77526163 | 77526086 | ID=cds.MS.gene48240.t1;Parent=MS.gene48240.t1 |
| Gene Sequence |
| Protein sequence |
