Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58403.t1 | XP_013457939.1 | 100 | 365 | 0 | 0 | 81 | 445 | 61 | 425 | 5.30E-215 | 756.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58403.t1 | Q56Y85 | 92.8 | 361 | 26 | 0 | 85 | 445 | 79 | 439 | 2.7e-204 | 712.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58403.t1 | A0A072V1I1 | 100.0 | 365 | 0 | 0 | 81 | 445 | 61 | 425 | 3.8e-215 | 756.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene93411 | MS.gene58403 | PPI |
| MS.gene064281 | MS.gene58403 | PPI |
| MS.gene45850 | MS.gene58403 | PPI |
| MS.gene48240 | MS.gene58403 | PPI |
| MS.gene044411 | MS.gene58403 | PPI |
| MS.gene039110 | MS.gene58403 | PPI |
| MS.gene060216 | MS.gene58403 | PPI |
| MS.gene48242 | MS.gene58403 | PPI |
| MS.gene91353 | MS.gene58403 | PPI |
| MS.gene58403 | MS.gene48237 | PPI |
| MS.gene58403 | MS.gene93411 | PPI |
| MS.gene063366 | MS.gene58403 | PPI |
| MS.gene48925 | MS.gene58403 | PPI |
| MS.gene066163 | MS.gene58403 | PPI |
| MS.gene040581 | MS.gene58403 | PPI |
| MS.gene93798 | MS.gene58403 | PPI |
| MS.gene63721 | MS.gene58403 | PPI |
| MS.gene033096 | MS.gene58403 | PPI |
| MS.gene58403 | MS.gene059218 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58403.t1 | MTR_4g109100 | 100.000 | 366 | 0 | 0 | 80 | 445 | 60 | 425 | 0.0 | 773 |
| MS.gene58403.t1 | MTR_1g105940 | 99.180 | 366 | 3 | 0 | 80 | 445 | 63 | 428 | 0.0 | 769 |
| MS.gene58403.t1 | MTR_1g105940 | 98.806 | 335 | 4 | 0 | 111 | 445 | 10 | 344 | 0.0 | 697 |
| MS.gene58403.t1 | MTR_2g078290 | 25.000 | 332 | 229 | 7 | 121 | 440 | 7 | 330 | 1.18e-28 | 116 |
| MS.gene58403.t1 | MTR_7g069390 | 25.602 | 332 | 213 | 8 | 121 | 433 | 7 | 323 | 2.39e-27 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58403.t1 | AT3G59990 | 92.562 | 363 | 27 | 0 | 83 | 445 | 77 | 439 | 0.0 | 731 |
| MS.gene58403.t1 | AT3G59990 | 92.562 | 363 | 27 | 0 | 83 | 445 | 77 | 439 | 0.0 | 731 |
| MS.gene58403.t1 | AT3G59990 | 92.562 | 363 | 27 | 0 | 83 | 445 | 77 | 439 | 0.0 | 731 |
| MS.gene58403.t1 | AT3G59990 | 92.562 | 363 | 27 | 0 | 83 | 445 | 77 | 439 | 0.0 | 731 |
| MS.gene58403.t1 | AT2G44180 | 87.056 | 394 | 51 | 0 | 52 | 445 | 48 | 441 | 0.0 | 713 |
| MS.gene58403.t1 | AT2G44180 | 90.966 | 321 | 29 | 0 | 125 | 445 | 1 | 321 | 0.0 | 625 |
| MS.gene58403.t1 | AT3G51800 | 26.549 | 339 | 215 | 8 | 121 | 440 | 8 | 331 | 2.80e-29 | 118 |
| MS.gene58403.t1 | AT3G51800 | 26.549 | 339 | 215 | 8 | 121 | 440 | 8 | 331 | 4.69e-29 | 117 |
| MS.gene58403.t1 | AT3G51800 | 26.149 | 348 | 214 | 9 | 121 | 440 | 8 | 340 | 7.34e-27 | 112 |
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAACTTTCAAAGTTATTA+TGG | 0.201569 | 8.3:+15527149 | MS.gene58403:intron |
| GATCTATCTATCCTTTAAAA+AGG | 0.225702 | 8.3:+15526621 | MS.gene58403:CDS |
| TCATGTAGTGGCTGCATTCC+AGG | 0.242992 | 8.3:-15535126 | None:intergenic |
| CTACGAACTGAATTATATAT+TGG | 0.245822 | 8.3:-15530856 | None:intergenic |
| TCATATGAGGTTGAGATTAA+TGG | 0.269675 | 8.3:+15534057 | MS.gene58403:CDS |
| ATTTCTATGCTAGTAACTTA+TGG | 0.289934 | 8.3:+15530788 | MS.gene58403:intron |
| TACTGCTGAATTTCACCTTC+TGG | 0.301917 | 8.3:-15529880 | None:intergenic |
| TTTCCAGGGTTGCCGCTCAT+TGG | 0.306132 | 8.3:+15532036 | MS.gene58403:intron |
| AACAGTCTTATCTCCAGAAT+TGG | 0.317380 | 8.3:-15532063 | None:intergenic |
| TTCTATGCAATTGAAACATT+CGG | 0.327183 | 8.3:+15534988 | MS.gene58403:CDS |
| GTTCTTGATCTTTACCCATC+TGG | 0.344235 | 8.3:+15529850 | MS.gene58403:CDS |
| AGCAACCTATACGGGTATTA+AGG | 0.345844 | 8.3:+15532319 | MS.gene58403:CDS |
| CGCTAATAGTTGCTTAGCTC+TGG | 0.346936 | 8.3:-15535185 | None:intergenic |
| CTCTAGGGAAGCAACCTATA+CGG | 0.355384 | 8.3:+15532310 | MS.gene58403:CDS |
| AGGAGAGACTAAATACTTAA+TGG | 0.355741 | 8.3:+15535264 | MS.gene58403:CDS |
| TCATTGCAGTTAAGAGTATA+CGG | 0.356486 | 8.3:+15534866 | MS.gene58403:intron |
| GTGTGATGCTGGCATTGTTC+AGG | 0.364503 | 8.3:+15535300 | MS.gene58403:CDS |
| CATATGAGGTTGAGATTAAT+GGG | 0.366320 | 8.3:+15534058 | MS.gene58403:CDS |
| GGTGGATCTGTCTGCTCAAC+GGG | 0.375413 | 8.3:-15529817 | None:intergenic |
| AAGTCGGACATCAGCATTCC+AGG | 0.376276 | 8.3:-15531438 | None:intergenic |
| CCACAGGATGCTCTCTGAAT+TGG | 0.378932 | 8.3:+15531532 | MS.gene58403:CDS |
| ACAGTCTTATCTCCAGAATT+GGG | 0.382436 | 8.3:-15532062 | None:intergenic |
| GATGTGATGAAGTTGGATTT+TGG | 0.396243 | 8.3:+15532098 | MS.gene58403:CDS |
| TCTATGCAATTGAAACATTC+GGG | 0.399479 | 8.3:+15534989 | MS.gene58403:CDS |
| AAGTACCTTAATACCCGTAT+AGG | 0.400679 | 8.3:-15532324 | None:intergenic |
| TGCTCTCCACCTTTCACAAT+GGG | 0.411459 | 8.3:-15534943 | None:intergenic |
| AGAGGAAGACAAGGAGCTTA+TGG | 0.412214 | 8.3:+15527194 | MS.gene58403:CDS |
| TGGTTTACCATTTAGGAAGC+TGG | 0.415762 | 8.3:+15533982 | MS.gene58403:intron |
| GATATAACTTCTTTGCATGT+TGG | 0.419203 | 8.3:-15535676 | None:intergenic |
| AGATCAAGAACAGGAATTGA+TGG | 0.426850 | 8.3:-15529838 | None:intergenic |
| ATTGAAACATTCGGGTCAAC+TGG | 0.430618 | 8.3:+15534997 | MS.gene58403:CDS |
| CAAGCAGTGGGTCAAACATT+GGG | 0.444461 | 8.3:-15532283 | None:intergenic |
| AGCAGCAGAAGTTCATCGTC+AGG | 0.444926 | 8.3:+15530879 | MS.gene58403:CDS |
| GCTGAAGAAATTATCTTCAA+AGG | 0.469330 | 8.3:+15524779 | MS.gene58403:CDS |
| CCCACTGCTTGAAGCCTCTA+GGG | 0.469534 | 8.3:+15532295 | MS.gene58403:CDS |
| TGCAAAGAAGTTATATCAAA+GGG | 0.471006 | 8.3:+15535682 | MS.gene58403:CDS |
| TATGCGGTCAGGAAGAATAA+AGG | 0.475430 | 8.3:+15529793 | MS.gene58403:intron |
| ATCTAAATATCGCCTACAAA+AGG | 0.477272 | 8.3:-15535236 | None:intergenic |
| GCTGGCATTGCTTTCCCCAC+AGG | 0.481828 | 8.3:+15531516 | MS.gene58403:CDS |
| TTAAGTATTTAGTCTCTCCT+AGG | 0.484045 | 8.3:-15535261 | None:intergenic |
| TCAAGCAGTGGGTCAAACAT+TGG | 0.485383 | 8.3:-15532284 | None:intergenic |
| ATGAAAGGCATTATAAAGCC+TGG | 0.486937 | 8.3:+15531420 | MS.gene58403:CDS |
| ACATCACATAGAGGAGGATA+AGG | 0.488058 | 8.3:-15535607 | None:intergenic |
| AACTTGGAAGATTTAACACT+TGG | 0.489193 | 8.3:+15524749 | MS.gene58403:CDS |
| AAGAGTATACGGAATTTGAA+TGG | 0.489615 | 8.3:+15534877 | MS.gene58403:CDS |
| TAACAAGAACTTCTCCACTT+TGG | 0.495871 | 8.3:+15535213 | MS.gene58403:CDS |
| TCCCTAGAGGCTTCAAGCAG+TGG | 0.496490 | 8.3:-15532296 | None:intergenic |
| GTATGATGATGTGATGAAGT+TGG | 0.503347 | 8.3:+15532091 | MS.gene58403:CDS |
| TTCAAAGTTATTATGGAGCA+AGG | 0.504540 | 8.3:+15527156 | MS.gene58403:CDS |
| GATGGGTAAAGATCAAGAAC+AGG | 0.505511 | 8.3:-15529847 | None:intergenic |
| TCAGAGGATGGCTTGCAAGC+TGG | 0.507428 | 8.3:+15531498 | MS.gene58403:CDS |
| CTTTGCATGTTGGTCGGAGT+AGG | 0.507622 | 8.3:-15535666 | None:intergenic |
| TCTAGGGAAGCAACCTATAC+GGG | 0.508969 | 8.3:+15532311 | MS.gene58403:CDS |
| TTGAAGAACTTGTGTGATGC+TGG | 0.511073 | 8.3:+15535289 | MS.gene58403:CDS |
| GAGCAGACAAAGATGGAAGA+GGG | 0.512947 | 8.3:+15534961 | MS.gene58403:CDS |
| ATGCAAAGAAGTTATATCAA+AGG | 0.517146 | 8.3:+15535681 | MS.gene58403:CDS |
| AGAGGTCATGGAGTCATATG+AGG | 0.524843 | 8.3:+15534044 | MS.gene58403:CDS |
| CTCCAATGTTTCACATAAGT+CGG | 0.525311 | 8.3:-15531454 | None:intergenic |
| AGGTGGAGAGCAGACAAAGA+TGG | 0.531180 | 8.3:+15534954 | MS.gene58403:CDS |
| GTGGCAGTAGCATGCAAGAA+AGG | 0.532651 | 8.3:+15527223 | MS.gene58403:CDS |
| CTTCCTTTCACATCACATAG+AGG | 0.537139 | 8.3:-15535616 | None:intergenic |
| TGCTGCAATTCAAGAGGTCA+TGG | 0.541118 | 8.3:+15534032 | MS.gene58403:CDS |
| AATTATATATTGGTTTCTGA+AGG | 0.541740 | 8.3:-15530846 | None:intergenic |
| CTGCTCTCCACCTTTCACAA+TGG | 0.543192 | 8.3:-15534944 | None:intergenic |
| CCCTAGAGGCTTCAAGCAGT+GGG | 0.545041 | 8.3:-15532295 | None:intergenic |
| TGGTGGATCTGTCTGCTCAA+CGG | 0.548615 | 8.3:-15529818 | None:intergenic |
| ATTAATGGGAAGGTGTATCA+AGG | 0.550038 | 8.3:+15534072 | MS.gene58403:CDS |
| AATTTGAATGGACATAGCAT+TGG | 0.550755 | 8.3:+15534889 | MS.gene58403:CDS |
| GATGTGCGCCTTTGTGATGT+TGG | 0.550983 | 8.3:+15534009 | MS.gene58403:CDS |
| ACCCACTGCTTGAAGCCTCT+AGG | 0.553042 | 8.3:+15532294 | MS.gene58403:CDS |
| TCCGTGTATTACCATCTATG+TGG | 0.557903 | 8.3:-15532124 | None:intergenic |
| TAACTTCTTTGCATGTTGGT+CGG | 0.562102 | 8.3:-15535672 | None:intergenic |
| AGAGCAGACAAAGATGGAAG+AGG | 0.562137 | 8.3:+15534960 | MS.gene58403:CDS |
| ATCAGAGGAGAAGAGAGAGC+TGG | 0.563096 | 8.3:+15530819 | MS.gene58403:CDS |
| AGCAGACAAAGATGGAAGAG+GGG | 0.576340 | 8.3:+15534962 | MS.gene58403:CDS |
| GACAAGGAGCTTATGGAGCA+AGG | 0.578268 | 8.3:+15527201 | MS.gene58403:CDS |
| CCTTCTGGAAAATCTCCAGA+TGG | 0.580900 | 8.3:-15529865 | None:intergenic |
| GGCCGCTATCAAATTCATGC+TGG | 0.582517 | 8.3:+15534910 | MS.gene58403:CDS |
| GCTAATAGTTGCTTAGCTCT+GGG | 0.588420 | 8.3:-15535184 | None:intergenic |
| GTCCGACTTATGTGAAACAT+TGG | 0.589857 | 8.3:+15531452 | MS.gene58403:CDS |
| TCTGTTCCCATTGTGAAAGG+TGG | 0.590439 | 8.3:+15534937 | MS.gene58403:CDS |
| CAGTCTTATCTCCAGAATTG+GGG | 0.590689 | 8.3:-15532061 | None:intergenic |
| CCGTGTATTACCATCTATGT+GGG | 0.591599 | 8.3:-15532123 | None:intergenic |
| TGAGGTTGAGATTAATGGGA+AGG | 0.591975 | 8.3:+15534062 | MS.gene58403:CDS |
| CCTCCTCTATGTGATGTGAA+AGG | 0.592331 | 8.3:+15535613 | MS.gene58403:CDS |
| CTCTGATATTAGCTTACGAA+CGG | 0.593227 | 8.3:-15531481 | None:intergenic |
| AGGTTATGTACGAGAAGACC+TGG | 0.594209 | 8.3:+15535108 | MS.gene58403:CDS |
| CGTATAGGTTGCTTCCCTAG+AGG | 0.600451 | 8.3:-15532309 | None:intergenic |
| AGGACTTGCAGAGGAAGACA+AGG | 0.601051 | 8.3:+15527185 | MS.gene58403:CDS |
| TCGCCTACAAAAGGCCAAAG+TGG | 0.603161 | 8.3:-15535227 | None:intergenic |
| TAGGTTCGGAAATATATGAA+AGG | 0.605556 | 8.3:+15531405 | MS.gene58403:intron |
| GCAGACAAAGATGGAAGAGG+GGG | 0.611179 | 8.3:+15534963 | MS.gene58403:CDS |
| CAATTCAGAGAGCATCCTGT+GGG | 0.612575 | 8.3:-15531531 | None:intergenic |
| TACCAGCATGAATTTGATAG+CGG | 0.613927 | 8.3:-15534912 | None:intergenic |
| AAACATTGGGTTGAACGCCA+CGG | 0.621240 | 8.3:-15532270 | None:intergenic |
| CGTAAGCTAATATCAGAGGA+TGG | 0.621543 | 8.3:+15531486 | MS.gene58403:CDS |
| TCAAGAACAGGAATTGATGG+TGG | 0.622681 | 8.3:-15529835 | None:intergenic |
| CGTTCGTAAGCTAATATCAG+AGG | 0.625420 | 8.3:+15531482 | MS.gene58403:CDS |
| ATTATGGAGCAAGGAAGACA+AGG | 0.630410 | 8.3:+15527165 | MS.gene58403:CDS |
| CCTTTCACATCACATAGAGG+AGG | 0.635044 | 8.3:-15535613 | None:intergenic |
| CTTCTGGAAAATCTCCAGAT+GGG | 0.635992 | 8.3:-15529864 | None:intergenic |
| AGGCAAGGACTTATTGAGCA+AGG | 0.642985 | 8.3:+15527243 | MS.gene58403:CDS |
| AGGAAGACAAGGACTTGCAG+AGG | 0.645505 | 8.3:+15527176 | MS.gene58403:CDS |
| AGGCGATATTTAGATCGCCT+AGG | 0.650391 | 8.3:+15535244 | MS.gene58403:CDS |
| AAATCTGTTCCCATTGTGAA+AGG | 0.658720 | 8.3:+15534934 | MS.gene58403:CDS |
| AGTAGCATGCAAGAAAGGCA+AGG | 0.663657 | 8.3:+15527228 | MS.gene58403:CDS |
| AGTCTTATCTCCAGAATTGG+GGG | 0.670396 | 8.3:-15532060 | None:intergenic |
| CCAATTCAGAGAGCATCCTG+TGG | 0.676893 | 8.3:-15531532 | None:intergenic |
| CTTATGGAGAACAACATCAG+AGG | 0.685730 | 8.3:+15530804 | MS.gene58403:CDS |
| TGTTGGTGCTGCAATTCAAG+AGG | 0.710293 | 8.3:+15534026 | MS.gene58403:CDS |
| TTGCAGCACCAACATCACAA+AGG | 0.716293 | 8.3:-15534017 | None:intergenic |
| AAGGAGCTTATGGAGCAAGG+TGG | 0.732569 | 8.3:+15527204 | MS.gene58403:CDS |
| TTAGCTCTGGGCAATCTCAG+CGG | 0.739474 | 8.3:-15535172 | None:intergenic |
| AATTCAGAGAGCATCCTGTG+GGG | 0.761775 | 8.3:-15531530 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 15524746 | 15535717 | 15524746 | ID=MS.gene58403 |
| chr8.3 | mRNA | 15524746 | 15535717 | 15524746 | ID=MS.gene58403.t1;Parent=MS.gene58403 |
| chr8.3 | exon | 15524746 | 15524800 | 15524746 | ID=MS.gene58403.t1.exon1;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15524746 | 15524800 | 15524746 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15526621 | 15526642 | 15526621 | ID=MS.gene58403.t1.exon2;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15526621 | 15526642 | 15526621 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15527152 | 15527264 | 15527152 | ID=MS.gene58403.t1.exon3;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15527152 | 15527264 | 15527152 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15529684 | 15529720 | 15529684 | ID=MS.gene58403.t1.exon4;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15529684 | 15529720 | 15529684 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15529804 | 15529911 | 15529804 | ID=MS.gene58403.t1.exon5;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15529804 | 15529911 | 15529804 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15530801 | 15530900 | 15530801 | ID=MS.gene58403.t1.exon6;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15530801 | 15530900 | 15530801 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15531408 | 15531553 | 15531408 | ID=MS.gene58403.t1.exon7;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15531408 | 15531553 | 15531408 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15532043 | 15532134 | 15532043 | ID=MS.gene58403.t1.exon8;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15532043 | 15532134 | 15532043 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15532246 | 15532340 | 15532246 | ID=MS.gene58403.t1.exon9;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15532246 | 15532340 | 15532246 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15533997 | 15534093 | 15533997 | ID=MS.gene58403.t1.exon10;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15533997 | 15534093 | 15533997 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15534875 | 15535018 | 15534875 | ID=MS.gene58403.t1.exon11;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15534875 | 15535018 | 15534875 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15535104 | 15535321 | 15535104 | ID=MS.gene58403.t1.exon12;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15535104 | 15535321 | 15535104 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| chr8.3 | exon | 15535607 | 15535717 | 15535607 | ID=MS.gene58403.t1.exon13;Parent=MS.gene58403.t1 |
| chr8.3 | CDS | 15535607 | 15535717 | 15535607 | ID=cds.MS.gene58403.t1;Parent=MS.gene58403.t1 |
| Gene Sequence |
| Protein sequence |
