Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91353.t1 | XP_013457939.1 | 99.3 | 425 | 3 | 0 | 1 | 425 | 1 | 425 | 5.00E-231 | 810.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91353.t1 | Q56Y85 | 92.5 | 361 | 27 | 0 | 65 | 425 | 79 | 439 | 5.8e-204 | 711.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91353.t1 | A0A072V1I1 | 99.3 | 425 | 3 | 0 | 1 | 425 | 1 | 425 | 3.6e-231 | 810.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene91353 | MS.gene060216 | PPI |
| MS.gene93798 | MS.gene91353 | PPI |
| MS.gene91353 | MS.gene039110 | PPI |
| MS.gene044411 | MS.gene91353 | PPI |
| MS.gene91353 | MS.gene63721 | PPI |
| MS.gene91353 | MS.gene58403 | PPI |
| MS.gene91353 | MS.gene059218 | PPI |
| MS.gene91353 | MS.gene45850 | PPI |
| MS.gene48242 | MS.gene91353 | PPI |
| MS.gene064281 | MS.gene91353 | PPI |
| MS.gene063366 | MS.gene91353 | PPI |
| MS.gene066163 | MS.gene91353 | PPI |
| MS.gene040581 | MS.gene91353 | PPI |
| MS.gene91353 | MS.gene48240 | PPI |
| MS.gene91353 | MS.gene48237 | PPI |
| MS.gene91353 | MS.gene93411 | PPI |
| MS.gene91353 | MS.gene033096 | PPI |
| MS.gene48925 | MS.gene91353 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91353.t1 | MTR_4g109100 | 99.294 | 425 | 3 | 0 | 1 | 425 | 1 | 425 | 0.0 | 879 |
| MS.gene91353.t1 | MTR_1g105940 | 97.664 | 428 | 7 | 1 | 1 | 425 | 1 | 428 | 0.0 | 868 |
| MS.gene91353.t1 | MTR_1g105940 | 98.806 | 335 | 4 | 0 | 91 | 425 | 10 | 344 | 0.0 | 698 |
| MS.gene91353.t1 | MTR_2g078290 | 25.000 | 332 | 229 | 7 | 101 | 420 | 7 | 330 | 9.30e-29 | 116 |
| MS.gene91353.t1 | MTR_7g069390 | 25.602 | 332 | 213 | 8 | 101 | 413 | 7 | 323 | 1.47e-27 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91353.t1 | AT3G59990 | 92.287 | 363 | 28 | 0 | 63 | 425 | 77 | 439 | 0.0 | 729 |
| MS.gene91353.t1 | AT3G59990 | 92.287 | 363 | 28 | 0 | 63 | 425 | 77 | 439 | 0.0 | 729 |
| MS.gene91353.t1 | AT3G59990 | 92.287 | 363 | 28 | 0 | 63 | 425 | 77 | 439 | 0.0 | 729 |
| MS.gene91353.t1 | AT3G59990 | 92.287 | 363 | 28 | 0 | 63 | 425 | 77 | 439 | 0.0 | 729 |
| MS.gene91353.t1 | AT2G44180 | 90.110 | 364 | 36 | 0 | 62 | 425 | 78 | 441 | 0.0 | 712 |
| MS.gene91353.t1 | AT2G44180 | 90.966 | 321 | 29 | 0 | 105 | 425 | 1 | 321 | 0.0 | 626 |
| MS.gene91353.t1 | AT3G51800 | 26.549 | 339 | 215 | 8 | 101 | 420 | 8 | 331 | 2.12e-29 | 118 |
| MS.gene91353.t1 | AT3G51800 | 26.549 | 339 | 215 | 8 | 101 | 420 | 8 | 331 | 3.02e-29 | 118 |
| MS.gene91353.t1 | AT3G51800 | 26.149 | 348 | 214 | 9 | 101 | 420 | 8 | 340 | 5.57e-27 | 112 |
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATGTAGTGGCTGCATTCC+AGG | 0.242992 | 1.3:-72467908 | None:intergenic |
| TCCTATGAGGTTGAGATTAA+TGG | 0.247977 | 1.3:+72466839 | MS.gene91353:CDS |
| CGACGAACTGAATTATATAT+TGG | 0.250887 | 1.3:-72463646 | None:intergenic |
| ATTTCTATGCTAGTAACTTA+TGG | 0.289934 | 1.3:+72463578 | MS.gene91353:intron |
| AACAGTCTTATCTCCGGAAT+TGG | 0.313628 | 1.3:-72464921 | None:intergenic |
| GGAAAGCAATGCCTGCTTGC+AGG | 0.317202 | 1.3:-72464363 | None:intergenic |
| TACTGCTGAATATCACCTTC+TGG | 0.326837 | 1.3:-72462455 | None:intergenic |
| GTTCTTGATCTTTACCCATC+TGG | 0.344235 | 1.3:+72462425 | MS.gene91353:CDS |
| AGCAACCTATACGGGTATTA+AGG | 0.345844 | 1.3:+72465177 | MS.gene91353:CDS |
| TTCTATGCAATTGAAACCTT+CGG | 0.354960 | 1.3:+72467770 | MS.gene91353:CDS |
| CTCTAGGGAAGCAACCTATA+CGG | 0.355384 | 1.3:+72465168 | MS.gene91353:CDS |
| AGGAGAGACTAAATACTTAA+TGG | 0.355741 | 1.3:+72468046 | MS.gene91353:CDS |
| TCATTGCAGTTAAGAGTATA+CGG | 0.356486 | 1.3:+72467648 | MS.gene91353:intron |
| GTGTGATGCTGGCATTGTTC+AGG | 0.364503 | 1.3:+72468082 | MS.gene91353:CDS |
| ACAGTCTTATCTCCGGAATT+GGG | 0.370525 | 1.3:-72464920 | None:intergenic |
| CCACAGGGTGCTCTCTGAAT+TGG | 0.373674 | 1.3:+72464386 | MS.gene91353:CDS |
| GGTGGATCTGTCTGCTCAAC+GGG | 0.375413 | 1.3:-72462392 | None:intergenic |
| AAGTCGGACATCAGCATTCC+AGG | 0.376276 | 1.3:-72464292 | None:intergenic |
| TTTCCAGGGTTGCTGCTCAC+TGG | 0.382312 | 1.3:+72464894 | MS.gene91353:intron |
| TCTATGCAATTGAAACCTTC+GGG | 0.389467 | 1.3:+72467771 | MS.gene91353:CDS |
| GATGTGATGAAGTTGGATTT+TGG | 0.396243 | 1.3:+72464956 | MS.gene91353:CDS |
| AAGTACCTTAATACCCGTAT+AGG | 0.400679 | 1.3:-72465182 | None:intergenic |
| CCTATGAGGTTGAGATTAAT+GGG | 0.409711 | 1.3:+72466840 | MS.gene91353:CDS |
| GATATAACTTCTTTGCAAGT+TGG | 0.409956 | 1.3:-72468454 | None:intergenic |
| TGCTCTCCACCTTTCACAAT+GGG | 0.411459 | 1.3:-72467725 | None:intergenic |
| AGATCAAGAACAGGAATTGA+TGG | 0.426850 | 1.3:-72462413 | None:intergenic |
| ATTGAAACCTTCGGGTCAAC+TGG | 0.432794 | 1.3:+72467779 | MS.gene91353:CDS |
| GCAGGCATTGCTTTCCCCAC+AGG | 0.445164 | 1.3:+72464370 | MS.gene91353:CDS |
| CCCATTAATCTCAACCTCAT+AGG | 0.455878 | 1.3:-72466840 | None:intergenic |
| GCTTCCTTTCACGTCACATA+GGG | 0.468088 | 1.3:-72468395 | None:intergenic |
| CCCACTGCTTGAAGCCTCTA+GGG | 0.469534 | 1.3:+72465153 | MS.gene91353:CDS |
| TGGCTTACCATTTAGGAAGC+TGG | 0.470912 | 1.3:+72466764 | MS.gene91353:intron |
| TGCAAAGAAGTTATATCAAA+GGG | 0.471006 | 1.3:+72468460 | MS.gene91353:CDS |
| ACGTCACATAGGGGAGGATA+AGG | 0.475041 | 1.3:-72468385 | None:intergenic |
| CAAGCAGTGGGTCAAACATC+GGG | 0.475650 | 1.3:-72465141 | None:intergenic |
| ATCTAAATATCGCCTACAAA+AGG | 0.477272 | 1.3:-72468018 | None:intergenic |
| AAATGTTATGTTCAGGGAAA+GGG | 0.481107 | 1.3:+72467871 | MS.gene91353:intron |
| CCTTCTTCTACAAGAGTTGG+TGG | 0.482897 | 1.3:-72458899 | None:intergenic |
| TTAAGTATTTAGTCTCTCCT+AGG | 0.484045 | 1.3:-72468043 | None:intergenic |
| ATGAAAGGCATTATAAAGCC+TGG | 0.486937 | 1.3:+72464274 | MS.gene91353:CDS |
| AAGAGTATACGGAATTTGAA+TGG | 0.489615 | 1.3:+72467659 | MS.gene91353:CDS |
| AGCAGCAGAAGTTCATCGTC+AGG | 0.491602 | 1.3:+72463669 | MS.gene91353:CDS |
| TAACAAGAACTTCTCCACTT+TGG | 0.495871 | 1.3:+72467995 | MS.gene91353:CDS |
| TCCCTAGAGGCTTCAAGCAG+TGG | 0.496490 | 1.3:-72465154 | None:intergenic |
| GTATGATGATGTGATGAAGT+TGG | 0.503347 | 1.3:+72464949 | MS.gene91353:CDS |
| GATGGGTAAAGATCAAGAAC+AGG | 0.505511 | 1.3:-72462422 | None:intergenic |
| TCTAGGGAAGCAACCTATAC+GGG | 0.508969 | 1.3:+72465169 | MS.gene91353:CDS |
| TTGAAGAACTTGTGTGATGC+TGG | 0.511073 | 1.3:+72468071 | MS.gene91353:CDS |
| TTCCGGTGATAGAGAAAGCT+CGG | 0.512296 | 1.3:-72458927 | None:intergenic |
| GAGCAGACAAAGATGGAAGA+GGG | 0.512947 | 1.3:+72467743 | MS.gene91353:CDS |
| TTGCAAAGAAGTTATATCAA+AGG | 0.517146 | 1.3:+72468459 | MS.gene91353:CDS |
| CTCCAATGTTTCACATAAGT+CGG | 0.525311 | 1.3:-72464308 | None:intergenic |
| CAGGCATTGCTTTCCCCACA+GGG | 0.526979 | 1.3:+72464371 | MS.gene91353:CDS |
| CTTTGCAAGTTGGTCGGAGT+AGG | 0.526988 | 1.3:-72468444 | None:intergenic |
| CGCCGAGCTTTCTCTATCAC+CGG | 0.527202 | 1.3:+72458925 | MS.gene91353:CDS |
| AGGTGGAGAGCAGACAAAGA+TGG | 0.531180 | 1.3:+72467736 | MS.gene91353:CDS |
| CAGTCTTATCTCCGGAATTG+GGG | 0.534987 | 1.3:-72464919 | None:intergenic |
| TCAGAGGATGGCCTGCAAGC+AGG | 0.539062 | 1.3:+72464352 | MS.gene91353:CDS |
| TGCTGCAATTCAAGAGGTCA+TGG | 0.541118 | 1.3:+72466814 | MS.gene91353:CDS |
| AATTATATATTGGTTTCTGA+AGG | 0.541740 | 1.3:-72463636 | None:intergenic |
| CTGCTCTCCACCTTTCACAA+TGG | 0.543192 | 1.3:-72467726 | None:intergenic |
| CCCTAGAGGCTTCAAGCAGT+GGG | 0.545041 | 1.3:-72465153 | None:intergenic |
| AGCTTCCTTTCACGTCACAT+AGG | 0.546614 | 1.3:-72468396 | None:intergenic |
| ACTCCTTCTTCTACAAGAGT+TGG | 0.546649 | 1.3:-72458902 | None:intergenic |
| TGGTGGATCTGTCTGCTCAA+CGG | 0.548615 | 1.3:-72462393 | None:intergenic |
| ATTAATGGGAAGGTGTATCA+AGG | 0.550038 | 1.3:+72466854 | MS.gene91353:CDS |
| AATTTGAATGGACATAGCAT+TGG | 0.550755 | 1.3:+72467671 | MS.gene91353:CDS |
| GATGTGCGCCTTTGTGATGT+TGG | 0.550983 | 1.3:+72466791 | MS.gene91353:CDS |
| ACCCACTGCTTGAAGCCTCT+AGG | 0.553042 | 1.3:+72465152 | MS.gene91353:CDS |
| CCACCAACTCTTGTAGAAGA+AGG | 0.555465 | 1.3:+72458899 | MS.gene91353:CDS |
| TCAAGCAGTGGGTCAAACAT+CGG | 0.558664 | 1.3:-72465142 | None:intergenic |
| TCCATGTATTACCATCTATG+TGG | 0.559923 | 1.3:-72464982 | None:intergenic |
| CTGAAGAACAGTCTTATCTC+CGG | 0.560346 | 1.3:-72464927 | None:intergenic |
| AGAGCAGACAAAGATGGAAG+AGG | 0.562137 | 1.3:+72467742 | MS.gene91353:CDS |
| ATCAGAGGAGAAGAGAGAGC+TGG | 0.563096 | 1.3:+72463609 | MS.gene91353:CDS |
| GCTAATAGTTGCTTAGCTCT+AGG | 0.564078 | 1.3:-72467966 | None:intergenic |
| ACAAGAAAATGGAGCTCAAG+AGG | 0.566879 | 1.3:+72458874 | MS.gene91353:CDS |
| TAACTTCTTTGCAAGTTGGT+CGG | 0.570212 | 1.3:-72468450 | None:intergenic |
| AGAGGTCATGGAGTCCTATG+AGG | 0.571277 | 1.3:+72466826 | MS.gene91353:CDS |
| AGCAGACAAAGATGGAAGAG+GGG | 0.576340 | 1.3:+72467744 | MS.gene91353:CDS |
| CCTTCTGGAAAATCTCCAGA+TGG | 0.580900 | 1.3:-72462440 | None:intergenic |
| GGCCGCTATCAAATTCATGC+TGG | 0.582517 | 1.3:+72467692 | MS.gene91353:CDS |
| CCTCCCCTATGTGACGTGAA+AGG | 0.585633 | 1.3:+72468391 | MS.gene91353:CDS |
| GTCCGACTTATGTGAAACAT+TGG | 0.589857 | 1.3:+72464306 | MS.gene91353:CDS |
| TCTGTTCCCATTGTGAAAGG+TGG | 0.590439 | 1.3:+72467719 | MS.gene91353:CDS |
| TCTGTGGTCAGGAAGAAGAA+AGG | 0.592872 | 1.3:+72462368 | MS.gene91353:intron |
| CTCTGATATTAGCTTACGAA+CGG | 0.593227 | 1.3:-72464335 | None:intergenic |
| TAGGTTCGGAAATATATGAA+AGG | 0.598735 | 1.3:+72464259 | MS.gene91353:intron |
| ACTATTACCAGTTGACCCGA+AGG | 0.599081 | 1.3:-72467786 | None:intergenic |
| CGTATAGGTTGCTTCCCTAG+AGG | 0.600451 | 1.3:-72465167 | None:intergenic |
| TGAGGTTGAGATTAATGGGA+AGG | 0.601845 | 1.3:+72466844 | MS.gene91353:CDS |
| AGGGTATGTACGAGAAGACC+TGG | 0.602085 | 1.3:+72467890 | MS.gene91353:CDS |
| CCATGTATTACCATCTATGT+GGG | 0.603110 | 1.3:-72464981 | None:intergenic |
| TCGCCTACAAAAGGCCAAAG+TGG | 0.603161 | 1.3:-72468009 | None:intergenic |
| GCAGACAAAGATGGAAGAGG+GGG | 0.611179 | 1.3:+72467745 | MS.gene91353:CDS |
| CAATTCAGAGAGCACCCTGT+GGG | 0.611392 | 1.3:-72464385 | None:intergenic |
| TACCAGCATGAATTTGATAG+CGG | 0.613927 | 1.3:-72467694 | None:intergenic |
| TCAAGAACAGGAATTGATGG+TGG | 0.622681 | 1.3:-72462410 | None:intergenic |
| CGTTCGTAAGCTAATATCAG+AGG | 0.625420 | 1.3:+72464336 | MS.gene91353:CDS |
| CTTCTGGAAAATCTCCAGAT+GGG | 0.635992 | 1.3:-72462439 | None:intergenic |
| CGTAAGCTAATATCAGAGGA+TGG | 0.640250 | 1.3:+72464340 | MS.gene91353:CDS |
| AGTCTTATCTCCGGAATTGG+GGG | 0.643385 | 1.3:-72464918 | None:intergenic |
| CCTTTCACGTCACATAGGGG+AGG | 0.648561 | 1.3:-72468391 | None:intergenic |
| AGGCGATATTTAGATCGCCT+AGG | 0.650391 | 1.3:+72468026 | MS.gene91353:CDS |
| CTTCCTTTCACGTCACATAG+GGG | 0.650914 | 1.3:-72468394 | None:intergenic |
| AAACATCGGGTTGAACGCCA+CGG | 0.651604 | 1.3:-72465128 | None:intergenic |
| AAATCTGTTCCCATTGTGAA+AGG | 0.658720 | 1.3:+72467716 | MS.gene91353:CDS |
| CTTATGGAGAACAACATCAG+AGG | 0.685730 | 1.3:+72463594 | MS.gene91353:CDS |
| AATTCAGAGAGCACCCTGTG+GGG | 0.705164 | 1.3:-72464384 | None:intergenic |
| TGTTGGTGCTGCAATTCAAG+AGG | 0.710293 | 1.3:+72466808 | MS.gene91353:CDS |
| TTGCAGCACCAACATCACAA+AGG | 0.716293 | 1.3:-72466799 | None:intergenic |
| CCAATTCAGAGAGCACCCTG+TGG | 0.742863 | 1.3:-72464386 | None:intergenic |
| TTAGCTCTAGGCAATCTCAG+CGG | 0.772254 | 1.3:-72467954 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 72458839 | 72468495 | 72458839 | ID=MS.gene91353 |
| chr1.3 | mRNA | 72458839 | 72468495 | 72458839 | ID=MS.gene91353.t1;Parent=MS.gene91353 |
| chr1.3 | exon | 72458839 | 72458968 | 72458839 | ID=MS.gene91353.t1.exon1;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72458839 | 72458968 | 72458839 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72462259 | 72462295 | 72462259 | ID=MS.gene91353.t1.exon2;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72462259 | 72462295 | 72462259 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72462379 | 72462486 | 72462379 | ID=MS.gene91353.t1.exon3;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72462379 | 72462486 | 72462379 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72463591 | 72463690 | 72463591 | ID=MS.gene91353.t1.exon4;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72463591 | 72463690 | 72463591 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72464262 | 72464407 | 72464262 | ID=MS.gene91353.t1.exon5;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72464262 | 72464407 | 72464262 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72464901 | 72464992 | 72464901 | ID=MS.gene91353.t1.exon6;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72464901 | 72464992 | 72464901 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72465104 | 72465198 | 72465104 | ID=MS.gene91353.t1.exon7;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72465104 | 72465198 | 72465104 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72466779 | 72466875 | 72466779 | ID=MS.gene91353.t1.exon8;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72466779 | 72466875 | 72466779 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72467657 | 72467800 | 72467657 | ID=MS.gene91353.t1.exon9;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72467657 | 72467800 | 72467657 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72467886 | 72468103 | 72467886 | ID=MS.gene91353.t1.exon10;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72467886 | 72468103 | 72467886 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| chr1.3 | exon | 72468385 | 72468495 | 72468385 | ID=MS.gene91353.t1.exon11;Parent=MS.gene91353.t1 |
| chr1.3 | CDS | 72468385 | 72468495 | 72468385 | ID=cds.MS.gene91353.t1;Parent=MS.gene91353.t1 |
| Gene Sequence |
| Protein sequence |
