Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48242.t1 | XP_024638078.1 | 99.2 | 397 | 3 | 0 | 1 | 397 | 1 | 397 | 1.60E-239 | 838.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48242.t1 | Q9SLN5 | 83.1 | 396 | 66 | 1 | 1 | 395 | 1 | 396 | 8.4e-205 | 714.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48242.t1 | A0A396I104 | 99.2 | 397 | 3 | 0 | 1 | 397 | 1 | 397 | 1.2e-239 | 838.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene93798 | MS.gene48242 | PPI |
| MS.gene48242 | MS.gene58403 | PPI |
| MS.gene48242 | MS.gene40160 | PPI |
| MS.gene48242 | MS.gene91353 | PPI |
| MS.gene044411 | MS.gene48242 | PPI |
| MS.gene48242 | MS.gene93411 | PPI |
| MS.gene48242 | MS.gene028573 | PPI |
| MS.gene48242 | MS.gene033096 | PPI |
| MS.gene48242 | MS.gene064281 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48242.t1 | MTR_5g017630 | 49.470 | 283 | 137 | 2 | 100 | 382 | 73 | 349 | 2.36e-92 | 281 |
| MS.gene48242.t1 | MTR_5g053940 | 47.703 | 283 | 142 | 2 | 100 | 382 | 78 | 354 | 8.28e-88 | 270 |
| MS.gene48242.t1 | MTR_5g053940 | 47.703 | 283 | 142 | 2 | 100 | 382 | 54 | 330 | 1.05e-87 | 269 |
| MS.gene48242.t1 | MTR_5g017630 | 48.945 | 237 | 115 | 2 | 100 | 336 | 73 | 303 | 4.09e-72 | 228 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48242.t1 | AT2G45240 | 82.872 | 397 | 67 | 1 | 1 | 396 | 1 | 397 | 0.0 | 705 |
| MS.gene48242.t1 | AT2G45240 | 80.685 | 321 | 61 | 1 | 1 | 320 | 1 | 321 | 0.0 | 559 |
| MS.gene48242.t1 | AT1G13270 | 48.763 | 283 | 139 | 2 | 100 | 382 | 91 | 367 | 2.28e-93 | 285 |
| MS.gene48242.t1 | AT4G37040 | 50.177 | 283 | 135 | 3 | 100 | 382 | 72 | 348 | 3.47e-91 | 278 |
| MS.gene48242.t1 | AT3G25740 | 45.196 | 281 | 143 | 4 | 105 | 382 | 69 | 341 | 6.27e-82 | 254 |
| MS.gene48242.t1 | AT1G13270 | 44.949 | 198 | 104 | 1 | 100 | 297 | 91 | 283 | 4.72e-55 | 183 |
| MS.gene48242.t1 | AT3G25740 | 43.333 | 120 | 59 | 2 | 105 | 222 | 69 | 181 | 7.26e-24 | 99.8 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTACGTGCAGCAAGATTT+TGG | 0.169908 | 4.2:-77499432 | None:intergenic |
| AGCTTCAATTCCATGCATTT+TGG | 0.176635 | 4.2:-77496361 | None:intergenic |
| CCCGTGAAGGTTCGGCATTC+TGG | 0.238200 | 4.2:+77496386 | MS.gene48242:CDS |
| GCACCAAACATTCCTCATTA+TGG | 0.269416 | 4.2:+77500805 | MS.gene48242:CDS |
| CATAATTAGGTGACAGAAAC+TGG | 0.285298 | 4.2:+77502654 | MS.gene48242:intron |
| ATAACTATTGGGCTCATCTT+TGG | 0.288590 | 4.2:-77498370 | None:intergenic |
| TTTCTCTCATTCTTTCAATC+TGG | 0.288753 | 4.2:-77498527 | None:intergenic |
| CAAAGCTTCCTTACTTTGAT+TGG | 0.291250 | 4.2:+77497864 | MS.gene48242:CDS |
| TCAATTTGAGCATACTCTTT+TGG | 0.293472 | 4.2:+77501393 | MS.gene48242:CDS |
| AATACATACTGAAGATTTGC+AGG | 0.300572 | 4.2:-77495656 | None:intergenic |
| GACAATCCGTTTACCAGATA+TGG | 0.303126 | 4.2:-77498155 | None:intergenic |
| CTCATATTGTTATACCTGAT+TGG | 0.325001 | 4.2:+77498182 | MS.gene48242:CDS |
| CTCATTCTTTCAATCTGGTC+TGG | 0.334220 | 4.2:-77498522 | None:intergenic |
| TCATATTGTGGTCATGGAAT+TGG | 0.341090 | 4.2:+77500766 | MS.gene48242:CDS |
| CATATACCTGCATTGATCAT+TGG | 0.346647 | 4.2:-77501233 | None:intergenic |
| TAACTATTGGGCTCATCTTT+GGG | 0.350866 | 4.2:-77498369 | None:intergenic |
| CTTCCATAATGAGGAATGTT+TGG | 0.353164 | 4.2:-77500808 | None:intergenic |
| CGTTTACCAGATATGGGATA+TGG | 0.372750 | 4.2:-77498148 | None:intergenic |
| AGTATGCTGCAGATAACTAT+TGG | 0.377305 | 4.2:-77498382 | None:intergenic |
| TTGCGGCCATATCCCATATC+TGG | 0.394058 | 4.2:+77498142 | MS.gene48242:CDS |
| TAATAACTTCGCCAATTTCA+CGG | 0.398419 | 4.2:-77500598 | None:intergenic |
| ATACTGAAGATTTGCAGGTT+TGG | 0.398456 | 4.2:-77495651 | None:intergenic |
| GGGGTACGGTTCCGTGAAAT+TGG | 0.404337 | 4.2:+77500587 | MS.gene48242:CDS |
| TCATATTGTTATACCTGATT+GGG | 0.406225 | 4.2:+77498183 | MS.gene48242:CDS |
| AATCGTCATGCTTCAATGTC+AGG | 0.408917 | 4.2:+77500620 | MS.gene48242:CDS |
| AGCACAAGAGAGTTTATTAA+CGG | 0.409837 | 4.2:-77495621 | None:intergenic |
| CTTCCTTACTTTGATTGGAC+TGG | 0.410698 | 4.2:+77497869 | MS.gene48242:CDS |
| CAATCAAAGTAAGGAAGCTT+TGG | 0.411005 | 4.2:-77497863 | None:intergenic |
| CTATCAAATTCAGTTAAACC+TGG | 0.424605 | 4.2:+77500566 | MS.gene48242:intron |
| GTGTACTATAAAGGTGTTCA+TGG | 0.440792 | 4.2:+77500026 | MS.gene48242:CDS |
| CACATATGAGTGCTTGGATA+AGG | 0.447878 | 4.2:+77500192 | MS.gene48242:CDS |
| GAAGCTTCCCCGTGAAGGTT+CGG | 0.449403 | 4.2:+77496378 | MS.gene48242:CDS |
| CTCACATCATCAGCCCAATC+AGG | 0.450396 | 4.2:-77498196 | None:intergenic |
| GATCATTGGTTCAATTGTAA+AGG | 0.450952 | 4.2:-77501219 | None:intergenic |
| GTATGCTGCAGATAACTATT+GGG | 0.452501 | 4.2:-77498381 | None:intergenic |
| AGATCGATCAACCTTGCATC+AGG | 0.454183 | 4.2:-77499564 | None:intergenic |
| CCAGAATGCCGAACCTTCAC+GGG | 0.456791 | 4.2:-77496386 | None:intergenic |
| CATATCCCATATCTGGTAAA+CGG | 0.459117 | 4.2:+77498149 | MS.gene48242:CDS |
| AAAAGTTCTTAATAGGTGTC+TGG | 0.459540 | 4.2:+77501311 | MS.gene48242:intron |
| TTCCTTACTTTGATTGGACT+GGG | 0.461313 | 4.2:+77497870 | MS.gene48242:CDS |
| ACCAGAATGCCGAACCTTCA+CGG | 0.461900 | 4.2:-77496387 | None:intergenic |
| TCTCAATGATACATACTTTG+TGG | 0.473493 | 4.2:+77500132 | MS.gene48242:CDS |
| CATCACCGAATGTTTATCCT+TGG | 0.478072 | 4.2:+77502706 | MS.gene48242:CDS |
| TTTAACCAAGGATAAACATT+CGG | 0.478433 | 4.2:-77502711 | None:intergenic |
| CTCAATGATACATACTTTGT+GGG | 0.479150 | 4.2:+77500133 | MS.gene48242:CDS |
| TACCCAGTCCAATCAAAGTA+AGG | 0.487570 | 4.2:-77497872 | None:intergenic |
| GTAAAATCATATTGTGGTCA+TGG | 0.489624 | 4.2:+77500760 | MS.gene48242:CDS |
| ACAATCCGTTTACCAGATAT+GGG | 0.495773 | 4.2:-77498154 | None:intergenic |
| AAATGGTAATTGAGAGGCGA+TGG | 0.498148 | 4.2:-77499405 | None:intergenic |
| GTAGATTGGAAGGGAGGTGT+TGG | 0.501126 | 4.2:+77499115 | MS.gene48242:intron |
| TCAGGTATAACAATATGAGC+TGG | 0.509419 | 4.2:-77498178 | None:intergenic |
| CACGAGGCAACTATTGCTGC+AGG | 0.513450 | 4.2:+77499197 | MS.gene48242:CDS |
| TATCAAATTCAGTTAAACCT+GGG | 0.516961 | 4.2:+77500567 | MS.gene48242:intron |
| CTGGCGTGATCGACTGTGGC+CGG | 0.519748 | 4.2:+77501330 | MS.gene48242:CDS |
| GTTAATGAAGTTATCTGCCA+TGG | 0.522636 | 4.2:+77499537 | MS.gene48242:CDS |
| GATGTTACCGTGTACTATAA+AGG | 0.523310 | 4.2:+77500017 | MS.gene48242:CDS |
| TCTCAGGTGTCCAAAATGCA+TGG | 0.531717 | 4.2:+77496351 | MS.gene48242:intron |
| CCCAATTATGAATTTAACCA+AGG | 0.537081 | 4.2:-77502723 | None:intergenic |
| ATCGACTGTGGCCGGATGGA+TGG | 0.541522 | 4.2:+77501338 | MS.gene48242:CDS |
| TGATTCGTAGATTGGAAGGG+AGG | 0.546142 | 4.2:+77499109 | MS.gene48242:intron |
| GCCGCTCGGATTATTCAGCC+TGG | 0.550022 | 4.2:+77499143 | MS.gene48242:CDS |
| GGTTATATTGCGTGAAGAGA+GGG | 0.555033 | 4.2:+77497828 | MS.gene48242:CDS |
| ATTCAGTTAAACCTGGGGTA+CGG | 0.555886 | 4.2:+77500573 | MS.gene48242:intron |
| CAAATGCACATATGAGTGCT+TGG | 0.558213 | 4.2:+77500186 | MS.gene48242:CDS |
| AAGAATGAGAGAAACATGTC+GGG | 0.558510 | 4.2:+77498536 | MS.gene48242:CDS |
| AAATTGATTCGTAGATTGGA+AGG | 0.558774 | 4.2:+77499105 | MS.gene48242:intron |
| AGGTGTTGGATGCAGCCGCT+CGG | 0.560148 | 4.2:+77499129 | MS.gene48242:CDS |
| ATTGAACCAATGATCAATGC+AGG | 0.561070 | 4.2:+77501227 | MS.gene48242:CDS |
| TAAAGCAGTTGGTGTGATGA+AGG | 0.565699 | 4.2:+77501187 | MS.gene48242:CDS |
| ATTTCACGGAACCGTACCCC+AGG | 0.565987 | 4.2:-77500584 | None:intergenic |
| GGATAAACATTCGGTGATGT+CGG | 0.569817 | 4.2:-77502702 | None:intergenic |
| GGGAAGAAATGGTAATTGAG+AGG | 0.572534 | 4.2:-77499411 | None:intergenic |
| CAGACTCACTTGGTGAAGGA+TGG | 0.573116 | 4.2:+77497807 | MS.gene48242:CDS |
| CAAAGGATACTTCCATAATG+AGG | 0.573541 | 4.2:-77500817 | None:intergenic |
| GTAACAGCAGTCCATCCATC+CGG | 0.573843 | 4.2:-77501349 | None:intergenic |
| ACACAGAACTCAGACTCACT+TGG | 0.575198 | 4.2:+77497797 | MS.gene48242:CDS |
| CAGGTATAACAATATGAGCT+GGG | 0.581087 | 4.2:-77498177 | None:intergenic |
| AAAGAATGAGAGAAACATGT+CGG | 0.582562 | 4.2:+77498535 | MS.gene48242:CDS |
| AATGGTAATTGAGAGGCGAT+GGG | 0.584764 | 4.2:-77499404 | None:intergenic |
| AAGGCAAAGGTATCCTCAGT+TGG | 0.584987 | 4.2:+77497773 | MS.gene48242:CDS |
| CGTGATCGACTGTGGCCGGA+TGG | 0.586768 | 4.2:+77501334 | MS.gene48242:CDS |
| GCCATGGAATTCCTGATGCA+AGG | 0.587828 | 4.2:+77499553 | MS.gene48242:CDS |
| ATTTCATCAGTTGTCACACC+AGG | 0.591424 | 4.2:-77499161 | None:intergenic |
| AACTCAGACTCACTTGGTGA+AGG | 0.594385 | 4.2:+77497803 | MS.gene48242:CDS |
| TGGTTATATTGCGTGAAGAG+AGG | 0.595369 | 4.2:+77497827 | MS.gene48242:CDS |
| GCAGTTGGTGTGATGAAGGC+TGG | 0.599210 | 4.2:+77501191 | MS.gene48242:CDS |
| TTCTACAGGAAGCTCGAGGA+TGG | 0.599342 | 4.2:+77499978 | MS.gene48242:intron |
| GGATGAAGAATCTCGTCAGT+TGG | 0.602205 | 4.2:+77500162 | MS.gene48242:CDS |
| ATATTGCGTGAAGAGAGGGC+AGG | 0.604163 | 4.2:+77497832 | MS.gene48242:CDS |
| TGGACTGCTGTTACAGCAGA+TGG | 0.605860 | 4.2:+77501358 | MS.gene48242:CDS |
| ATCAGTGCATCTGAAGGCAA+AGG | 0.606762 | 4.2:+77497760 | MS.gene48242:CDS |
| GCACAAATCAGTGCATCTGA+AGG | 0.608305 | 4.2:+77497754 | MS.gene48242:CDS |
| CTGGCAGGAAATAAAGCAGT+TGG | 0.619503 | 4.2:+77501176 | MS.gene48242:intron |
| CTGCTCTTGCAAGGGCATTG+CGG | 0.623478 | 4.2:+77498125 | MS.gene48242:intron |
| ATCAAATTCAGTTAAACCTG+GGG | 0.627357 | 4.2:+77500568 | MS.gene48242:intron |
| TTATCTGCAGCATACTGTTG+AGG | 0.627462 | 4.2:+77498389 | MS.gene48242:CDS |
| AATTGATTCGTAGATTGGAA+GGG | 0.630358 | 4.2:+77499106 | MS.gene48242:intron |
| GAATTGAAGCTTCCCCGTGA+AGG | 0.631728 | 4.2:+77496373 | MS.gene48242:CDS |
| TATTGCGTGAAGAGAGGGCA+GGG | 0.632056 | 4.2:+77497833 | MS.gene48242:CDS |
| TTGGATAAGGCAATATCCAT+CGG | 0.639566 | 4.2:+77500205 | MS.gene48242:CDS |
| TACATACTTTGTGGGAAACG+TGG | 0.647305 | 4.2:+77500141 | MS.gene48242:CDS |
| GTCATTCTACAGGAAGCTCG+AGG | 0.647941 | 4.2:+77499974 | MS.gene48242:intron |
| GAGGAATGTTTGGTGCACAA+TGG | 0.651672 | 4.2:-77500798 | None:intergenic |
| TTACAGGTAAAATCATATTG+TGG | 0.652206 | 4.2:+77500754 | MS.gene48242:intron |
| ATGAACACCTTTATAGTACA+CGG | 0.658395 | 4.2:-77500024 | None:intergenic |
| ACCTTGCATCAGGAATTCCA+TGG | 0.669846 | 4.2:-77499554 | None:intergenic |
| TGAGTTCTGTGTGCCAACTG+AGG | 0.676738 | 4.2:-77497786 | None:intergenic |
| GTGTCTGGCGTGATCGACTG+TGG | 0.700621 | 4.2:+77501326 | MS.gene48242:CDS |
| CAGAATGCCGAACCTTCACG+GGG | 0.704929 | 4.2:-77496385 | None:intergenic |
| TTCAATGTCAGGCTTCTCAG+TGG | 0.712844 | 4.2:+77500631 | MS.gene48242:CDS |
| ACCAGGCTGAATAATCCGAG+CGG | 0.746152 | 4.2:-77499144 | None:intergenic |
| AATTGACAGAGTAGTTCACG+AGG | 0.767513 | 4.2:+77499181 | MS.gene48242:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 77495599 | 77502740 | 77495599 | ID=MS.gene48242 |
| chr4.2 | mRNA | 77495599 | 77502740 | 77495599 | ID=MS.gene48242.t1;Parent=MS.gene48242 |
| chr4.2 | exon | 77495599 | 77495669 | 77495599 | ID=MS.gene48242.t1.exon1;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77495599 | 77495669 | 77495599 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77496357 | 77496407 | 77496357 | ID=MS.gene48242.t1.exon2;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77496357 | 77496407 | 77496357 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77497721 | 77497891 | 77497721 | ID=MS.gene48242.t1.exon3;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77497721 | 77497891 | 77497721 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77498138 | 77498213 | 77498138 | ID=MS.gene48242.t1.exon4;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77498138 | 77498213 | 77498138 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77498363 | 77498410 | 77498363 | ID=MS.gene48242.t1.exon5;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77498363 | 77498410 | 77498363 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77498513 | 77498557 | 77498513 | ID=MS.gene48242.t1.exon6;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77498513 | 77498557 | 77498513 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77499119 | 77499218 | 77499119 | ID=MS.gene48242.t1.exon7;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77499119 | 77499218 | 77499119 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77499397 | 77499448 | 77499397 | ID=MS.gene48242.t1.exon8;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77499397 | 77499448 | 77499397 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77499533 | 77499574 | 77499533 | ID=MS.gene48242.t1.exon9;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77499533 | 77499574 | 77499533 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77499986 | 77500047 | 77499986 | ID=MS.gene48242.t1.exon10;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77499986 | 77500047 | 77499986 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77500128 | 77500226 | 77500128 | ID=MS.gene48242.t1.exon11;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77500128 | 77500226 | 77500128 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77500579 | 77500652 | 77500579 | ID=MS.gene48242.t1.exon12;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77500579 | 77500652 | 77500579 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77500760 | 77500829 | 77500760 | ID=MS.gene48242.t1.exon13;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77500760 | 77500829 | 77500760 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77501183 | 77501248 | 77501183 | ID=MS.gene48242.t1.exon14;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77501183 | 77501248 | 77501183 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77501326 | 77501414 | 77501326 | ID=MS.gene48242.t1.exon15;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77501326 | 77501414 | 77501326 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| chr4.2 | exon | 77502663 | 77502740 | 77502663 | ID=MS.gene48242.t1.exon16;Parent=MS.gene48242.t1 |
| chr4.2 | CDS | 77502663 | 77502740 | 77502663 | ID=cds.MS.gene48242.t1;Parent=MS.gene48242.t1 |
| Gene Sequence |
| Protein sequence |
