AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48822

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48822.t1 MTR_4g028960 96.517 603 21 0 1 603 99 701 0.0 1180
MS.gene48822.t1 MTR_2g018010 38.185 584 328 11 6 567 103 675 8.73e-119 369
MS.gene48822.t1 MTR_5g083030 30.732 628 358 14 1 570 80 688 4.90e-81 269
MS.gene48822.t1 MTR_4g107010 31.115 601 355 16 1 568 92 666 1.62e-77 261
MS.gene48822.t1 MTR_3g078160 29.932 588 378 12 1 568 86 659 1.04e-73 249
MS.gene48822.t1 MTR_3g078160 29.932 588 378 12 1 568 86 659 1.11e-73 249
MS.gene48822.t1 MTR_1g094215 32.927 574 354 15 1 567 71 620 1.54e-67 231
MS.gene48822.t1 MTR_3g078160 33.333 456 277 9 1 443 86 527 4.94e-67 228
MS.gene48822.t1 MTR_4g485520 31.759 614 305 21 4 567 99 648 7.34e-67 230
MS.gene48822.t1 MTR_4g485520 39.356 404 203 13 180 567 103 480 1.16e-66 225
MS.gene48822.t1 MTR_1g094215 38.520 392 219 10 181 567 63 437 2.51e-63 215
MS.gene48822.t1 MTR_4g063800 31.023 577 367 15 5 567 69 628 2.43e-62 218
MS.gene48822.t1 MTR_7g078330 31.228 570 355 13 9 567 99 642 1.33e-61 216
MS.gene48822.t1 MTR_7g059405 37.113 388 223 9 181 565 263 632 6.45e-60 211
MS.gene48822.t1 MTR_5g034440 28.426 591 387 14 1 568 90 667 4.63e-58 207
MS.gene48822.t1 MTR_7g053260 33.419 389 239 10 183 567 79 451 6.26e-51 182
MS.gene48822.t1 MTR_7g116600 33.162 389 241 10 183 567 79 452 1.82e-48 176
MS.gene48822.t1 MTR_1g079450 31.486 397 248 10 173 562 54 433 1.32e-42 159
MS.gene48822.t1 MTR_3g085610 25.968 439 258 9 183 566 281 707 2.61e-37 148
MS.gene48822.t1 MTR_8g080280 27.027 444 249 13 183 567 283 710 7.92e-36 144
MS.gene48822.t1 MTR_2g011140 29.765 383 243 8 187 566 20 379 2.29e-30 123
MS.gene48822.t1 MTR_4g091880 28.718 390 246 8 182 566 5 367 6.94e-27 113
MS.gene48822.t1 MTR_4g085720 26.415 424 254 14 182 580 14 404 2.59e-26 112
MS.gene48822.t1 MTR_8g092870 25.666 413 263 14 178 566 6 398 1.81e-25 109
MS.gene48822.t1 MTR_4g091880 27.919 394 216 12 182 566 5 339 6.65e-25 106
MS.gene48822.t1 MTR_3g466220 55.952 84 36 1 172 254 222 305 2.73e-24 108
MS.gene48822.t1 MTR_3g466220 35.424 271 161 8 308 574 574 834 1.91e-21 99.4
MS.gene48822.t1 MTR_3g466220 55.952 84 36 1 172 254 201 284 3.09e-24 108
MS.gene48822.t1 MTR_3g466220 35.424 271 161 8 308 574 553 813 2.26e-21 99.4
MS.gene48822.t1 MTR_3g065080 25.610 328 187 10 179 461 33 348 4.81e-24 105
MS.gene48822.t1 MTR_7g097020 33.117 308 181 10 276 574 472 763 5.51e-24 107
MS.gene48822.t1 MTR_7g097020 59.420 69 28 0 186 254 245 313 1.01e-23 106
MS.gene48822.t1 MTR_1g069845 37.500 152 82 2 101 249 50 191 9.28e-24 106
MS.gene48822.t1 MTR_1g069845 32.237 304 183 12 280 574 317 606 3.28e-21 98.2
MS.gene48822.t1 MTR_1g069845 62.687 67 25 0 183 249 246 312 2.13e-23 105
MS.gene48822.t1 MTR_1g069845 32.119 302 186 11 280 574 438 727 2.27e-21 99.0
MS.gene48822.t1 MTR_1g069845 62.687 67 25 0 183 249 242 308 2.40e-23 105
MS.gene48822.t1 MTR_1g069845 32.119 302 186 11 280 574 434 723 2.12e-21 99.4
MS.gene48822.t1 MTR_5g048050 51.948 77 37 0 177 253 20 96 7.69e-23 102
MS.gene48822.t1 MTR_6g071340 51.316 76 37 0 178 253 1 76 2.11e-22 100
MS.gene48822.t1 MTR_5g020570 23.128 454 278 12 175 566 281 725 2.42e-22 102
MS.gene48822.t1 MTR_5g020570 23.128 454 278 12 175 566 266 710 2.59e-22 102
MS.gene48822.t1 MTR_5g077510 47.368 76 40 0 178 253 31 106 3.44e-22 100
MS.gene48822.t1 MTR_8g077205 21.105 507 307 11 129 566 226 708 5.47e-22 101
MS.gene48822.t1 MTR_8g077205 21.105 507 307 11 129 566 114 596 5.83e-22 100
MS.gene48822.t1 MTR_2g087350 32.243 214 121 7 179 386 3 198 7.80e-22 98.6
MS.gene48822.t1 MTR_3g103050 29.965 287 191 6 285 567 50 330 5.30e-21 95.1
MS.gene48822.t1 MTR_1g093965 28.977 176 103 3 195 370 1 154 5.37e-21 91.7
MS.gene48822.t1 MTR_3g095730 25.659 417 256 19 178 566 9 399 1.97e-20 94.4
MS.gene48822.t1 MTR_7g106340 29.139 302 172 13 179 459 5 285 2.48e-20 94.4
MS.gene48822.t1 MTR_3g096370 51.429 70 34 0 179 248 7 76 6.77e-20 92.8
MS.gene48822.t1 MTR_1g093995 28.409 176 104 3 195 370 1 154 1.53e-19 87.4
MS.gene48822.t1 MTR_1g018180 32.143 280 165 8 298 567 72 336 3.43e-19 89.7
MS.gene48822.t1 MTR_1g056840 27.795 331 163 15 175 459 2 302 1.37e-18 89.0
MS.gene48822.t1 MTR_5g015210 25.606 371 222 12 176 528 60 394 2.86e-18 88.2
MS.gene48822.t1 MTR_1g056880 27.744 328 167 14 175 459 2 302 5.14e-18 87.4
MS.gene48822.t1 MTR_1g056870 37.725 167 74 8 175 332 2 147 2.91e-17 85.1
MS.gene48822.t1 MTR_1g017770 29.515 227 116 8 182 398 45 237 1.67e-16 82.8
MS.gene48822.t1 MTR_1g056910 49.333 75 36 2 179 251 3 77 3.90e-16 81.3
MS.gene48822.t1 MTR_2g026130 31.900 279 145 10 308 566 196 449 4.64e-16 81.3
MS.gene48822.t1 MTR_1g100820 50.000 66 33 0 183 248 649 714 5.14e-16 82.0
MS.gene48822.t1 MTR_1g100820 50.000 66 33 0 183 248 647 712 5.79e-16 82.0
MS.gene48822.t1 MTR_8g039560 30.449 312 181 12 275 565 1 297 6.68e-16 79.7
MS.gene48822.t1 MTR_7g077780 47.143 70 37 0 183 252 827 896 3.44e-15 79.7
MS.gene48822.t1 MTR_7g117890 27.692 260 148 9 9 248 82 321 1.08e-14 78.2
MS.gene48822.t1 MTR_4g051515 33.544 158 86 7 179 333 3 144 2.55e-14 75.9
MS.gene48822.t1 MTR_8g027140 22.489 458 292 15 9 450 83 493 7.67e-14 75.5
MS.gene48822.t1 MTR_4g125930 46.377 69 36 1 183 251 492 559 1.67e-13 73.9
MS.gene48822.t1 MTR_4g125930 46.377 69 36 1 183 251 692 759 2.42e-13 73.6
MS.gene48822.t1 MTR_3g115670 25.000 388 217 14 165 484 14 395 3.54e-13 73.2
MS.gene48822.t1 MTR_5g032010 44.776 67 37 0 182 248 732 798 4.90e-13 72.8
MS.gene48822.t1 MTR_2g096850 44.000 75 41 1 177 250 584 658 6.98e-13 72.0
MS.gene48822.t1 MTR_2g096850 44.000 75 41 1 177 250 735 809 7.36e-13 72.0
MS.gene48822.t1 MTR_1g090320 43.056 72 37 3 183 252 514 583 1.52e-11 68.2
MS.gene48822.t1 MTR_1g090320 43.056 72 37 3 183 252 516 585 1.58e-11 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48822.t1 AT5G01830 55.868 605 232 7 1 601 96 669 0.0 653
MS.gene48822.t1 AT1G29340 35.313 623 361 11 6 596 115 727 2.79e-111 350
MS.gene48822.t1 AT1G60190 29.933 598 363 16 1 568 94 665 1.05e-70 241
MS.gene48822.t1 AT3G54850 32.124 579 353 20 1 567 72 622 3.52e-68 233
MS.gene48822.t1 AT5G42340 31.119 572 348 16 9 567 118 656 9.88e-66 227
MS.gene48822.t1 AT3G46510 30.225 579 363 14 9 569 76 631 1.08e-65 227
MS.gene48822.t1 AT1G23030 32.522 575 311 17 14 567 91 609 7.85e-65 224
MS.gene48822.t1 AT1G10560 28.289 608 370 16 1 568 95 676 4.82e-61 215
MS.gene48822.t1 AT1G71020 35.142 424 230 10 167 567 71 472 7.95e-60 207
MS.gene48822.t1 AT2G28830 29.630 567 366 12 19 568 13 563 2.11e-59 208
MS.gene48822.t1 AT1G71020 31.661 578 314 17 16 567 98 620 1.68e-58 207
MS.gene48822.t1 AT3G07360 32.927 410 246 13 171 567 59 452 2.55e-49 178
MS.gene48822.t1 AT1G67530 26.906 669 367 23 1 566 57 706 1.76e-40 157
MS.gene48822.t1 AT1G67530 26.906 669 367 23 1 566 57 706 1.76e-40 157
MS.gene48822.t1 AT1G67530 26.906 669 367 23 1 566 57 706 1.76e-40 157
MS.gene48822.t1 AT1G27910 26.471 442 268 10 173 566 271 703 7.13e-36 144
MS.gene48822.t1 AT4G21350 32.308 390 231 10 182 565 5 367 2.69e-33 131
MS.gene48822.t1 AT1G24330 23.565 662 397 17 1 566 57 705 3.44e-33 135
MS.gene48822.t1 AT1G24330 23.565 662 397 17 1 566 57 705 3.44e-33 135
MS.gene48822.t1 AT1G67530 27.586 464 226 18 1 369 57 505 2.16e-28 120
MS.gene48822.t1 AT5G18320 28.856 402 253 12 182 571 72 452 3.02e-28 118
MS.gene48822.t1 AT1G49780 29.238 407 253 12 178 566 10 399 1.38e-27 115
MS.gene48822.t1 AT3G19380 29.006 362 223 12 178 522 10 354 2.20e-27 115
MS.gene48822.t1 AT1G66160 25.393 445 253 17 175 586 26 424 1.07e-24 107
MS.gene48822.t1 AT5G67340 33.208 265 158 8 309 566 445 697 2.21e-24 108
MS.gene48822.t1 AT5G67340 31.148 244 139 8 18 254 91 312 4.81e-21 98.2
MS.gene48822.t1 AT5G67340 33.208 265 158 8 309 566 445 697 2.21e-24 108
MS.gene48822.t1 AT5G67340 31.148 244 139 8 18 254 91 312 4.81e-21 98.2
MS.gene48822.t1 AT5G37490 24.939 413 255 14 179 565 28 411 1.00e-23 104
MS.gene48822.t1 AT2G23140 30.153 262 139 8 19 266 83 314 3.48e-23 105
MS.gene48822.t1 AT2G23140 32.979 282 175 9 297 574 553 824 1.92e-22 102
MS.gene48822.t1 AT2G23140 30.153 262 139 8 19 266 83 314 3.48e-23 105
MS.gene48822.t1 AT2G23140 32.979 282 175 9 297 574 553 824 1.92e-22 102
MS.gene48822.t1 AT2G23140 30.153 262 139 8 19 266 86 317 3.65e-23 105
MS.gene48822.t1 AT2G23140 32.624 282 176 8 297 574 556 827 1.76e-22 102
MS.gene48822.t1 AT3G54790 54.795 73 33 0 182 254 202 274 3.07e-22 102
MS.gene48822.t1 AT3G54790 30.515 272 158 7 308 566 459 712 1.07e-18 90.9
MS.gene48822.t1 AT3G54790 54.795 73 33 0 182 254 238 310 3.96e-22 101
MS.gene48822.t1 AT3G54790 30.515 272 158 7 308 566 495 748 6.35e-19 91.7
MS.gene48822.t1 AT3G54790 54.795 73 33 0 182 254 238 310 3.96e-22 101
MS.gene48822.t1 AT3G54790 30.515 272 158 7 308 566 495 748 6.35e-19 91.7
MS.gene48822.t1 AT2G23140 32.979 282 175 9 297 574 364 635 4.17e-22 101
MS.gene48822.t1 AT2G23140 42.105 95 55 0 172 266 31 125 5.07e-22 100
MS.gene48822.t1 AT2G23140 32.979 282 175 9 297 574 364 635 4.17e-22 101
MS.gene48822.t1 AT2G23140 42.105 95 55 0 172 266 31 125 5.07e-22 100
MS.gene48822.t1 AT5G64660 50.667 75 37 0 174 248 2 76 4.63e-21 96.7
MS.gene48822.t1 AT3G49810 25.967 362 219 10 182 528 64 391 1.21e-20 95.5
MS.gene48822.t1 AT5G18340 27.458 295 191 9 171 457 63 342 9.26e-20 92.8
MS.gene48822.t1 AT5G65920 24.658 365 220 11 182 528 60 387 1.17e-19 92.4
MS.gene48822.t1 AT5G65920 24.658 365 220 11 182 528 60 387 1.17e-19 92.4
MS.gene48822.t1 AT3G01400 29.508 305 182 8 285 575 63 348 1.22e-19 91.3
MS.gene48822.t1 AT3G52450 52.778 72 33 1 179 249 4 75 1.26e-19 92.4
MS.gene48822.t1 AT2G35930 25.414 362 209 13 179 510 9 339 1.40e-19 92.0
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G07360 28.829 333 212 12 244 567 1 317 2.06e-19 90.1
MS.gene48822.t1 AT3G18710 50.725 69 34 0 180 248 10 78 3.61e-19 90.9
MS.gene48822.t1 AT5G09800 52.239 67 32 0 182 248 11 77 4.11e-19 90.5
MS.gene48822.t1 AT5G58680 29.856 278 181 7 295 567 71 339 3.05e-17 84.0
MS.gene48822.t1 AT1G01660 41.304 92 54 0 157 248 473 564 8.93e-17 84.3
MS.gene48822.t1 AT1G01660 41.304 92 54 0 157 248 472 563 9.15e-17 84.3
MS.gene48822.t1 AT1G66160 23.596 445 245 18 175 586 26 408 6.62e-16 80.9
MS.gene48822.t1 AT2G45910 40.449 89 53 0 164 252 745 833 2.60e-15 80.1
MS.gene48822.t1 AT2G45910 40.449 89 53 0 164 252 745 833 2.60e-15 80.1
MS.gene48822.t1 AT1G01680 44.737 76 42 0 173 248 224 299 6.41e-15 76.3
MS.gene48822.t1 AT2G45920 48.485 66 34 0 183 248 326 391 9.08e-15 77.0
MS.gene48822.t1 AT3G11840 44.737 76 40 2 179 253 7 81 7.94e-14 74.3
MS.gene48822.t1 AT1G08315 26.911 327 193 13 277 577 4 310 1.78e-13 72.4
MS.gene48822.t1 AT1G56040 46.032 63 34 0 183 245 354 416 3.92e-13 72.0
MS.gene48822.t1 AT3G49060 41.892 74 43 0 175 248 728 801 5.39e-13 72.8
MS.gene48822.t1 AT3G49060 41.892 74 43 0 175 248 718 791 5.40e-13 72.8
MS.gene48822.t1 AT3G49060 41.892 74 43 0 175 248 695 768 5.50e-13 72.4
MS.gene48822.t1 AT5G57035 43.939 66 37 0 183 248 709 774 1.24e-12 71.6
MS.gene48822.t1 AT3G61390 43.939 66 37 0 183 248 279 344 1.92e-12 69.7
MS.gene48822.t1 AT3G61390 43.939 66 37 0 183 248 354 419 3.29e-12 69.3
MS.gene48822.t1 AT4G16490 30.466 279 151 10 308 566 206 461 5.05e-12 68.9
MS.gene48822.t1 AT2G19410 44.928 69 37 1 180 248 709 776 1.34e-11 68.2
MS.gene48822.t1 AT2G19410 44.928 69 37 1 180 248 723 790 1.41e-11 68.2
MS.gene48822.t1 AT2G19410 44.928 69 37 1 180 248 744 811 1.46e-11 68.2
MS.gene48822.t1 AT5G40140 26.165 279 181 10 299 566 242 506 4.01e-11 66.2

Find 154 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAGCTTTGGATGGATTC+TGG 0.199858 4.4:+73894018 MS.gene48822:CDS
AGCGGGAGCTATAGAGATTT+TGG 0.243976 4.4:+73894359 MS.gene48822:CDS
TTCAAGTTCATTTCTTGAAT+TGG 0.244410 4.4:-73893529 None:intergenic
GATAATTTCAACAAATTAAC+AGG 0.267688 4.4:+73893478 MS.gene48822:CDS
AAGGGTGATTTGGGAGTTAA+TGG 0.283097 4.4:+73895004 MS.gene48822:CDS
TGAGAAATATGATAGCTATT+TGG 0.284416 4.4:+73894106 MS.gene48822:CDS
GCTACTGTGTTTAGTTTAAC+TGG 0.293033 4.4:+73894570 MS.gene48822:CDS
AATACCACCGTTAACTTTAA+CGG 0.306403 4.4:-73894169 None:intergenic
ACGTCGGTGGGCGGCGGGTT+TGG 0.311205 4.4:+73895079 MS.gene48822:CDS
CGGTGTTGTTTATGAGCTTC+GGG 0.314495 4.4:+73894293 MS.gene48822:CDS
TGGTGTGGAAAGGGTGATTT+GGG 0.315581 4.4:+73894995 MS.gene48822:CDS
TGGTGGTTCCGGCGGATTAC+CGG 0.317373 4.4:+73893923 MS.gene48822:CDS
GGAGATGACAGCAGGGATTA+TGG 0.329171 4.4:+73894761 MS.gene48822:CDS
ATGGTGTGGAAAGGGTGATT+TGG 0.329462 4.4:+73894994 MS.gene48822:CDS
CAGGAAATGATGGCCGTTTA+CGG 0.344485 4.4:+73893881 MS.gene48822:CDS
TTAAGCTTTGGATGGATTCT+GGG 0.361071 4.4:+73894019 MS.gene48822:CDS
ACGGTGTTGTTTATGAGCTT+CGG 0.362241 4.4:+73894292 MS.gene48822:CDS
AAAATTGGGGACGGTGTTAA+GGG 0.365091 4.4:+73894875 MS.gene48822:CDS
AAATGTATATTGTGCTTCAT+AGG 0.366592 4.4:+73893383 None:intergenic
AAGTGAGATTGAAATTCTTC+AGG 0.368437 4.4:+73893750 MS.gene48822:CDS
CTGCTGGAAGATTGTTCTAA+TGG 0.369769 4.4:+73893415 MS.gene48822:CDS
GTCGTTGGAATTGGTGGTTC+CGG 0.372795 4.4:+73893912 MS.gene48822:CDS
CGCATCAGTTCAAGACTTAT+CGG 0.383935 4.4:-73893949 None:intergenic
GAACAGAAAATTCCATTTGA+AGG 0.385995 4.4:+73894135 MS.gene48822:CDS
ATCGTGTTTCTATTAAGCTT+TGG 0.388631 4.4:+73894007 MS.gene48822:CDS
AGCGAATAAGACAAGGATAA+TGG 0.391442 4.4:+73894470 MS.gene48822:CDS
GCGGAATCAGTCGTTGGAAT+TGG 0.393295 4.4:+73893903 MS.gene48822:CDS
GAACAGAAACAACATCATTT+CGG 0.395400 4.4:-73893636 None:intergenic
ATGGCGGTGGGGAGTGTTAG+AGG 0.398283 4.4:+73895023 MS.gene48822:CDS
GCGGAGCTTGCGGCAATTCA+TGG 0.399152 4.4:+73894975 MS.gene48822:CDS
GATCCTGTCGTCGTGGCAAC+AGG 0.400951 4.4:+73893973 MS.gene48822:CDS
TCAGAAACGAAGAGTTTGTT+AGG 0.401404 4.4:+73893595 MS.gene48822:CDS
GATTTGGGAGTTAATGGCGG+TGG 0.402348 4.4:+73895010 MS.gene48822:CDS
GTATTAACTCCGTGTGGGCC+AGG 0.406263 4.4:-73894071 None:intergenic
GATGTTGTTTCTGTTCTTGA+AGG 0.418829 4.4:+73893643 MS.gene48822:CDS
TGGGCGGCGGGTTTGGATGG+TGG 0.427448 4.4:+73895086 MS.gene48822:CDS
GGCGGTAATGCCGGAGGAGG+CGG 0.437841 4.4:+73894782 MS.gene48822:CDS
ACGACTGATTCCGCCGTAAA+CGG 0.442880 4.4:-73893894 None:intergenic
AAAAGAAATTGTTCCGAAAG+AGG 0.454422 4.4:+73893672 MS.gene48822:CDS
TATTACAAATAAGACAGCTT+TGG 0.454524 4.4:+73894188 MS.gene48822:CDS
GGTTAAGCGTGGTGGATTAG+TGG 0.454539 4.4:+73894824 MS.gene48822:CDS
GGGAGGGATCAGAGAGGGCG+AGG 0.456168 4.4:+73894895 MS.gene48822:CDS
CACAACACGTGTCCTAAAAC+AGG 0.458381 4.4:+73894042 MS.gene48822:CDS
CGGTGGGGAGTGTTAGAGGG+AGG 0.459782 4.4:+73895027 MS.gene48822:CDS
GAACTTGAATGATGATGTAT+TGG 0.460758 4.4:+73893543 MS.gene48822:CDS
AGCGAAGAGGGACGCATTGG+CGG 0.461449 4.4:+73894677 MS.gene48822:CDS
TTTGCATCTTGCAGCGGATA+GGG 0.461754 4.4:+73894704 MS.gene48822:CDS
TCATAGGATTAAAACTCTGC+TGG 0.463244 4.4:+73893399 MS.gene48822:CDS
ACGGTTGCCGTTAAAGTTAA+CGG 0.472054 4.4:+73894162 MS.gene48822:CDS
ATTTGGGAGTTAATGGCGGT+GGG 0.475030 4.4:+73895011 MS.gene48822:CDS
ATATTTCTCAAAGCATGGTT+AGG 0.475353 4.4:-73894093 None:intergenic
GGGGCCGTGGAGATGACAGC+AGG 0.477716 4.4:+73894753 MS.gene48822:CDS
TTCAGGAAGAAATTCAGAAT+CGG 0.479614 4.4:+73893767 MS.gene48822:CDS
GCGTCCCTCTTCGCTCCCTC+AGG 0.480363 4.4:-73894669 None:intergenic
GCAACCGTTTCACCTTCAAA+TGG 0.483942 4.4:-73894147 None:intergenic
AATTCCATTTGAAGGTGAAA+CGG 0.485444 4.4:+73894143 MS.gene48822:CDS
TGTCCTGTTGCCACGACGAC+AGG 0.493205 4.4:-73893976 None:intergenic
AGTATCGTCACCGCCTCCTC+CGG 0.493654 4.4:-73894792 None:intergenic
TCAGAGATTGTTGCGTTGAT+CGG 0.493804 4.4:+73893808 MS.gene48822:CDS
GAGTGTTAGAGGGAGGAGGA+AGG 0.494415 4.4:+73895034 MS.gene48822:CDS
CGTTAGTGGATTGGTTGAGT+TGG 0.496851 4.4:+73894635 MS.gene48822:CDS
TGTGCTAATGAAGTTGTAGT+TGG 0.496874 4.4:-73895170 None:intergenic
TGTTTCTATTAAGCTTTGGA+TGG 0.497575 4.4:+73894011 MS.gene48822:CDS
GTTGGTAGAGAGTGGGGCCG+TGG 0.498825 4.4:+73894740 MS.gene48822:CDS
AGGGATTATGGCGGTAATGC+CGG 0.499026 4.4:+73894773 MS.gene48822:CDS
GGGTTTGGATGGTGGTGAAA+CGG 0.499778 4.4:+73895094 MS.gene48822:CDS
TATTAACTCCGTGTGGGCCA+GGG 0.501113 4.4:-73894070 None:intergenic
GACACGAATCGTTAGTGGAT+TGG 0.503002 4.4:+73894626 MS.gene48822:CDS
CGGTGGGCGGCGGGTTTGGA+TGG 0.505339 4.4:+73895083 MS.gene48822:CDS
TGTGTTTAGTTTAACTGGAG+TGG 0.506633 4.4:+73894575 MS.gene48822:CDS
AGGAAGACACGAATCGTTAG+TGG 0.516476 4.4:+73894621 MS.gene48822:CDS
TGGTGGATTAGTGGCGGTTG+CGG 0.516629 4.4:+73894833 MS.gene48822:CDS
GAATCAGTCGTTGGAATTGG+TGG 0.520402 4.4:+73893906 MS.gene48822:CDS
TAGGGAGACGGTGGGAAGGT+TGG 0.520508 4.4:+73894722 MS.gene48822:CDS
TCGAGCTTGTATAGCTGAAG+CGG 0.522415 4.4:+73894341 MS.gene48822:CDS
TGGGGAGTGTTAGAGGGAGG+AGG 0.524460 4.4:+73895030 MS.gene48822:CDS
GGAGGGATCAGAGAGGGCGA+GGG 0.524599 4.4:+73894896 MS.gene48822:CDS
GGTGCAACGACGGCGTCGAC+AGG 0.525335 4.4:+73893862 MS.gene48822:CDS
TAACGGTTCTTTAGCATCGA+TGG 0.527793 4.4:+73894260 MS.gene48822:CDS
TTCGCAAAATCCGAGCCTAC+AGG 0.528569 4.4:+73894407 MS.gene48822:CDS
TATGGCGGTAATGCCGGAGG+AGG 0.529298 4.4:+73894779 MS.gene48822:CDS
ATTGGGGACGGTGTTAAGGG+AGG 0.534820 4.4:+73894878 MS.gene48822:CDS
TGGACCTGAGGGAGCGAAGA+GGG 0.535333 4.4:+73894665 MS.gene48822:CDS
GATAAGTCTTGAACTGATGC+GGG 0.544369 4.4:+73893951 MS.gene48822:CDS
AGAGATTGTGGCGGAGCTTG+CGG 0.545486 4.4:+73894965 MS.gene48822:CDS
GGGAGCGAAGAGGGACGCAT+TGG 0.546419 4.4:+73894674 MS.gene48822:CDS
TTGGATGGTGGTGAAACGGA+GGG 0.546989 4.4:+73895098 MS.gene48822:CDS
TGGGAAGGTTGGTAGAGAGT+GGG 0.549322 4.4:+73894733 MS.gene48822:CDS
GGAAGGCGGCGACGTTGTTG+AGG 0.554359 4.4:+73895051 MS.gene48822:CDS
CGATAAGTCTTGAACTGATG+CGG 0.556842 4.4:+73893950 MS.gene48822:CDS
CGGCAATTCATGGTGTGGAA+AGG 0.558496 4.4:+73894985 MS.gene48822:CDS
GTGGGAAGGTTGGTAGAGAG+TGG 0.562347 4.4:+73894732 MS.gene48822:CDS
CGAGCTTGTATAGCTGAAGC+GGG 0.563020 4.4:+73894342 MS.gene48822:CDS
CTATCATATTTCTCAAAGCA+TGG 0.564762 4.4:-73894098 None:intergenic
ATCGGACACCGGTAATCCGC+CGG 0.567196 4.4:-73893931 None:intergenic
GGCAATTCATGGTGTGGAAA+GGG 0.572572 4.4:+73894986 MS.gene48822:CDS
AGAGAGGGCGAGGGAGAGCG+CGG 0.576119 4.4:+73894905 MS.gene48822:CDS
TGGATTAGTGGCGGTTGCGG+CGG 0.579291 4.4:+73894836 MS.gene48822:CDS
GGGAAGGTTGGTAGAGAGTG+GGG 0.582589 4.4:+73894734 MS.gene48822:CDS
GGTGATTTGGGAGTTAATGG+CGG 0.585091 4.4:+73895007 MS.gene48822:CDS
CGGATAGGGAGACGGTGGGA+AGG 0.585973 4.4:+73894718 MS.gene48822:CDS
GTTGGAATTGGTGGTTCCGG+CGG 0.589268 4.4:+73893915 MS.gene48822:CDS
TGTTAGAGGGAGGAGGAAGG+CGG 0.591852 4.4:+73895037 MS.gene48822:CDS
ACGGCGTCGACAGGAAATGA+TGG 0.591925 4.4:+73893871 MS.gene48822:CDS
TCCTAAAACAGGTCAAACCC+TGG 0.592266 4.4:+73894053 MS.gene48822:CDS
AGGTCAAACCCTGGCCCACA+CGG 0.592317 4.4:+73894062 MS.gene48822:CDS
TTTGGATGGTGGTGAAACGG+AGG 0.595577 4.4:+73895097 MS.gene48822:CDS
TTTACGGCGGAATCAGTCGT+TGG 0.597846 4.4:+73893897 MS.gene48822:CDS
GGTTAGGTATTAACTCCGTG+TGG 0.601915 4.4:-73894077 None:intergenic
TGGCGGTGGGGAGTGTTAGA+GGG 0.603345 4.4:+73895024 MS.gene48822:CDS
TGTTAGTACGTCAACTACCA+TGG 0.603963 4.4:+73895142 MS.gene48822:CDS
TCTTGCAGCGGATAGGGAGA+CGG 0.605026 4.4:+73894710 MS.gene48822:CDS
AACAAGTTGACGTTGACTAA+CGG 0.605175 4.4:+73894243 MS.gene48822:CDS
GAAAGGTGGATCAGAGATTG+TGG 0.605941 4.4:+73894953 MS.gene48822:CDS
ACTGCATTAACCTGTAGGCT+CGG 0.609903 4.4:-73894417 None:intergenic
GTTAAGGGAGGGATCAGAGA+GGG 0.610803 4.4:+73894890 MS.gene48822:CDS
TTTAAGATCGGGTGCCACGT+GGG 0.611826 4.4:+73894527 MS.gene48822:CDS
TTGTAACTGCATTAACCTGT+AGG 0.615427 4.4:-73894422 None:intergenic
TTGGGGACGGTGTTAAGGGA+GGG 0.620299 4.4:+73894879 MS.gene48822:CDS
TGCAGCGGATAGGGAGACGG+TGG 0.621227 4.4:+73894713 MS.gene48822:CDS
GCATCGATGGAAGAGACAAA+CGG 0.622184 4.4:+73894273 MS.gene48822:CDS
TTTGGAAGCAACAAGAATGA+CGG 0.625454 4.4:+73894206 MS.gene48822:CDS
GCTTGCGGCAATTCATGGTG+TGG 0.630345 4.4:+73894980 MS.gene48822:CDS
AAGATCGGGTGCCACGTGGG+AGG 0.630647 4.4:+73894530 MS.gene48822:CDS
GATTATGGCGGTAATGCCGG+AGG 0.631080 4.4:+73894776 MS.gene48822:CDS
CGACACTTGTGACAATGTGT+AGG 0.637316 4.4:+73894931 MS.gene48822:CDS
TAATCCACCACGCTTAACCA+CGG 0.638766 4.4:-73894820 None:intergenic
TTTGGGAGTTAATGGCGGTG+GGG 0.641101 4.4:+73895012 MS.gene48822:CDS
CTTGAAGCCGTGGTTAAGCG+TGG 0.643099 4.4:+73894813 MS.gene48822:CDS
GGTGACGATACTTGAAGCCG+TGG 0.646261 4.4:+73894803 MS.gene48822:CDS
CTTGTGACAATGTGTAGGAA+AGG 0.647925 4.4:+73894936 MS.gene48822:CDS
GAAATGATGGCCGTTTACGG+CGG 0.648185 4.4:+73893884 MS.gene48822:CDS
GTGGGAGGCTAAAGCGAATG+CGG 0.649553 4.4:+73894545 MS.gene48822:CDS
GTTAGGTATTAACTCCGTGT+GGG 0.650428 4.4:-73894076 None:intergenic
ACAAGGATAATGGAGACTGA+AGG 0.650963 4.4:+73894480 MS.gene48822:CDS
GTGACAATGTGTAGGAAAGG+TGG 0.655053 4.4:+73894939 MS.gene48822:CDS
GGGCCGTGGAGATGACAGCA+GGG 0.655928 4.4:+73894754 MS.gene48822:CDS
TACTTGAAGCGAATAAGACA+AGG 0.661909 4.4:+73894463 MS.gene48822:CDS
GCAGCGGATAGGGAGACGGT+GGG 0.662717 4.4:+73894714 MS.gene48822:CDS
AATCCCTGCTGTCATCTCCA+CGG 0.665782 4.4:-73894757 None:intergenic
ATGGACCTGAGGGAGCGAAG+AGG 0.672284 4.4:+73894664 MS.gene48822:CDS
GGAGGCTAAAGCGAATGCGG+CGG 0.672752 4.4:+73894548 MS.gene48822:CDS
GAAGCCGTGGTTAAGCGTGG+TGG 0.680940 4.4:+73894816 MS.gene48822:CDS
TAAGCGTGGTGGATTAGTGG+CGG 0.684918 4.4:+73894827 MS.gene48822:CDS
GATGCGGGATCCTGTCGTCG+TGG 0.685189 4.4:+73893966 MS.gene48822:CDS
GTTCAAGACTTATCGGACAC+CGG 0.688916 4.4:-73893942 None:intergenic
AGTTGTAGTTGGAACAACCA+TGG 0.693372 4.4:-73895159 None:intergenic
ACACATTGTCACAAGTGTCG+CGG 0.696733 4.4:-73894928 None:intergenic
AGGTGGATCAGAGATTGTGG+CGG 0.710219 4.4:+73894956 MS.gene48822:CDS
ATTCGCTTTAGCCTCCCACG+TGG 0.725438 4.4:-73894541 None:intergenic
TGTTAAGGGAGGGATCAGAG+AGG 0.725744 4.4:+73894889 MS.gene48822:CDS
GGAAGCAACAAGAATGACGG+CGG 0.729408 4.4:+73894209 MS.gene48822:CDS
GATGACAGCAGGGATTATGG+CGG 0.739268 4.4:+73894764 MS.gene48822:CDS
GTTGCCGTTAAAGTTAACGG+TGG 0.741567 4.4:+73894165 MS.gene48822:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATTTCAACAAATTAAC+AGG + chr4.4:73893478-73893497 MS.gene48822:CDS 20.0%
!! TTTGTAGGGATAAAAAAATT+GGG + chr4.4:73894861-73894880 MS.gene48822:CDS 20.0%
!!! ATCTTCAATTTTTGAGAAAT+TGG + chr4.4:73893702-73893721 MS.gene48822:CDS 20.0%
!!! TTTTGTAGGGATAAAAAAAT+TGG + chr4.4:73894860-73894879 MS.gene48822:CDS 20.0%
! TATTACAAATAAGACAGCTT+TGG + chr4.4:73894188-73894207 MS.gene48822:CDS 25.0%
! TGAGAAATATGATAGCTATT+TGG + chr4.4:73894106-73894125 MS.gene48822:CDS 25.0%
! TTGTAGGGATAAAAAAATTG+GGG + chr4.4:73894862-73894881 MS.gene48822:CDS 25.0%
!! GAAGATAATTTTTCCTCTTT+CGG - chr4.4:73893688-73893707 None:intergenic 25.0%
!! TTCAAGTTCATTTCTTGAAT+TGG - chr4.4:73893532-73893551 None:intergenic 25.0%
AAAAGAAATTGTTCCGAAAG+AGG + chr4.4:73893672-73893691 MS.gene48822:CDS 30.0%
AAGTGAGATTGAAATTCTTC+AGG + chr4.4:73893750-73893769 MS.gene48822:CDS 30.0%
AATACCACCGTTAACTTTAA+CGG - chr4.4:73894172-73894191 None:intergenic 30.0%
AATTCCATTTGAAGGTGAAA+CGG + chr4.4:73894143-73894162 MS.gene48822:CDS 30.0%
ATATTTCTCAAAGCATGGTT+AGG - chr4.4:73894096-73894115 None:intergenic 30.0%
CTATCATATTTCTCAAAGCA+TGG - chr4.4:73894101-73894120 None:intergenic 30.0%
GAACAGAAAATTCCATTTGA+AGG + chr4.4:73894135-73894154 MS.gene48822:CDS 30.0%
GAACAGAAACAACATCATTT+CGG - chr4.4:73893639-73893658 None:intergenic 30.0%
TTCAGGAAGAAATTCAGAAT+CGG + chr4.4:73893767-73893786 MS.gene48822:CDS 30.0%
! ATCGTGTTTCTATTAAGCTT+TGG + chr4.4:73894007-73894026 MS.gene48822:CDS 30.0%
! GAACTTGAATGATGATGTAT+TGG + chr4.4:73893543-73893562 MS.gene48822:CDS 30.0%
!! TGTTTCTATTAAGCTTTGGA+TGG + chr4.4:73894011-73894030 MS.gene48822:CDS 30.0%
!!! CGGTTTTTAAGTTATGAAGT+TGG + chr4.4:73894384-73894403 MS.gene48822:CDS 30.0%
AGCGAATAAGACAAGGATAA+TGG + chr4.4:73894470-73894489 MS.gene48822:CDS 35.0%
AGGGATAAAAAAATTGGGGA+CGG + chr4.4:73894866-73894885 MS.gene48822:CDS 35.0%
TACTTGAAGCGAATAAGACA+AGG + chr4.4:73894463-73894482 MS.gene48822:CDS 35.0%
TCATAGGATTAAAACTCTGC+TGG + chr4.4:73893399-73893418 MS.gene48822:CDS 35.0%
TGTGCTAATGAAGTTGTAGT+TGG - chr4.4:73895173-73895192 None:intergenic 35.0%
TTGTAACTGCATTAACCTGT+AGG - chr4.4:73894425-73894444 None:intergenic 35.0%
TTTGGAAGCAACAAGAATGA+CGG + chr4.4:73894206-73894225 MS.gene48822:CDS 35.0%
! AACAAGTTGACGTTGACTAA+CGG + chr4.4:73894243-73894262 MS.gene48822:CDS 35.0%
! GCTACTGTGTTTAGTTTAAC+TGG + chr4.4:73894570-73894589 MS.gene48822:CDS 35.0%
! TCAGAAACGAAGAGTTTGTT+AGG + chr4.4:73893595-73893614 MS.gene48822:CDS 35.0%
! TGTGTTTAGTTTAACTGGAG+TGG + chr4.4:73894575-73894594 MS.gene48822:CDS 35.0%
!! ATTAAGCTTTGGATGGATTC+TGG + chr4.4:73894018-73894037 MS.gene48822:CDS 35.0%
!! GATGTTGTTTCTGTTCTTGA+AGG + chr4.4:73893643-73893662 MS.gene48822:CDS 35.0%
!! GGTTTTGTCGAAATCAGATT+CGG + chr4.4:73894314-73894333 MS.gene48822:CDS 35.0%
!! TTAAGCTTTGGATGGATTCT+GGG + chr4.4:73894019-73894038 MS.gene48822:CDS 35.0%
!!! GAGCTATAGAGATTTTGGTT+CGG + chr4.4:73894364-73894383 MS.gene48822:CDS 35.0%
!!! TGTAGCAGAGGTTTTAAGAT+CGG + chr4.4:73894515-73894534 MS.gene48822:CDS 35.0%
ACGGTTGCCGTTAAAGTTAA+CGG + chr4.4:73894162-73894181 MS.gene48822:CDS 40.0%
AGTTGTAGTTGGAACAACCA+TGG - chr4.4:73895162-73895181 None:intergenic 40.0%
CGATAAGTCTTGAACTGATG+CGG + chr4.4:73893950-73893969 MS.gene48822:CDS 40.0%
CGCATCAGTTCAAGACTTAT+CGG - chr4.4:73893952-73893971 None:intergenic 40.0%
CTGCTGGAAGATTGTTCTAA+TGG + chr4.4:73893415-73893434 MS.gene48822:CDS 40.0%
CTTGTGACAATGTGTAGGAA+AGG + chr4.4:73894936-73894955 MS.gene48822:CDS 40.0%
GATAAGTCTTGAACTGATGC+GGG + chr4.4:73893951-73893970 MS.gene48822:CDS 40.0%
GTTAGGTATTAACTCCGTGT+GGG - chr4.4:73894079-73894098 None:intergenic 40.0%
TGTTAGTACGTCAACTACCA+TGG + chr4.4:73895142-73895161 MS.gene48822:CDS 40.0%
! AAAAATTGGGGACGGTGTTA+AGG + chr4.4:73894874-73894893 MS.gene48822:CDS 40.0%
! AAAATTGGGGACGGTGTTAA+GGG + chr4.4:73894875-73894894 MS.gene48822:CDS 40.0%
! AAGGGTGATTTGGGAGTTAA+TGG + chr4.4:73895004-73895023 MS.gene48822:CDS 40.0%
! CGTTGTTGAGGATTTTACGT+CGG + chr4.4:73895063-73895082 MS.gene48822:CDS 40.0%
! TAACGGTTCTTTAGCATCGA+TGG + chr4.4:73894260-73894279 MS.gene48822:CDS 40.0%
! TATGCGAAGTGCGTTCTTTT+TGG + chr4.4:73893841-73893860 MS.gene48822:CDS 40.0%
! TCAGAGATTGTTGCGTTGAT+CGG + chr4.4:73893808-73893827 MS.gene48822:CDS 40.0%
!! ACAAGGATAATGGAGACTGA+AGG + chr4.4:73894480-73894499 MS.gene48822:CDS 40.0%
!! ACGGTGTTGTTTATGAGCTT+CGG + chr4.4:73894292-73894311 MS.gene48822:CDS 40.0%
!! CTTTTAGAAAGCGGTTAGGT+AGG + chr4.4:73894601-73894620 MS.gene48822:CDS 40.0%
!! TGTTTTAAGTGGTGTAGCAG+AGG + chr4.4:73894503-73894522 MS.gene48822:CDS 40.0%
!! TTGGTTGAGTTGGCGAAAAA+TGG + chr4.4:73894645-73894664 MS.gene48822:CDS 40.0%
!!! GAGACTGAAGGTGTTTTAAG+TGG + chr4.4:73894492-73894511 MS.gene48822:CDS 40.0%
!!! GAGTGGTTGCTTTTAGAAAG+CGG + chr4.4:73894592-73894611 MS.gene48822:CDS 40.0%
!!! GTAGCAGAGGTTTTAAGATC+GGG + chr4.4:73894516-73894535 MS.gene48822:CDS 40.0%
!!! GTTGCTTTTAGAAAGCGGTT+AGG + chr4.4:73894597-73894616 MS.gene48822:CDS 40.0%
ACTGCATTAACCTGTAGGCT+CGG - chr4.4:73894420-73894439 None:intergenic 45.0%
AGGAAGACACGAATCGTTAG+TGG + chr4.4:73894621-73894640 MS.gene48822:CDS 45.0%
ATGGTGTGGAAAGGGTGATT+TGG + chr4.4:73894994-73895013 MS.gene48822:CDS 45.0%
ATTTGGGAGTTAATGGCGGT+GGG + chr4.4:73895011-73895030 MS.gene48822:CDS 45.0%
CACAACACGTGTCCTAAAAC+AGG + chr4.4:73894042-73894061 MS.gene48822:CDS 45.0%
CAGGAAATGATGGCCGTTTA+CGG + chr4.4:73893881-73893900 MS.gene48822:CDS 45.0%
CGACACTTGTGACAATGTGT+AGG + chr4.4:73894931-73894950 MS.gene48822:CDS 45.0%
GAAAGGTGGATCAGAGATTG+TGG + chr4.4:73894953-73894972 MS.gene48822:CDS 45.0%
GAATCAGTCGTTGGAATTGG+TGG + chr4.4:73893906-73893925 MS.gene48822:CDS 45.0%
GCAACCGTTTCACCTTCAAA+TGG - chr4.4:73894150-73894169 None:intergenic 45.0%
GGCAATTCATGGTGTGGAAA+GGG + chr4.4:73894986-73895005 MS.gene48822:CDS 45.0%
GGTTAGGTATTAACTCCGTG+TGG - chr4.4:73894080-73894099 None:intergenic 45.0%
GTGACAATGTGTAGGAAAGG+TGG + chr4.4:73894939-73894958 MS.gene48822:CDS 45.0%
GTTCAAGACTTATCGGACAC+CGG - chr4.4:73893945-73893964 None:intergenic 45.0%
GTTGCCGTTAAAGTTAACGG+TGG + chr4.4:73894165-73894184 MS.gene48822:CDS 45.0%
TAATCCACCACGCTTAACCA+CGG - chr4.4:73894823-73894842 None:intergenic 45.0%
TCCTAAAACAGGTCAAACCC+TGG + chr4.4:73894053-73894072 MS.gene48822:CDS 45.0%
TCGAGCTTGTATAGCTGAAG+CGG + chr4.4:73894341-73894360 MS.gene48822:CDS 45.0%
TGGTGTGGAAAGGGTGATTT+GGG + chr4.4:73894995-73895014 MS.gene48822:CDS 45.0%
TTGGCGAAAAATGGACCTGA+GGG + chr4.4:73894654-73894673 MS.gene48822:CDS 45.0%
TTTGCATCTTGCAGCGGATA+GGG + chr4.4:73894704-73894723 MS.gene48822:CDS 45.0%
! ACACATTGTCACAAGTGTCG+CGG - chr4.4:73894931-73894950 None:intergenic 45.0%
! AGCGGGAGCTATAGAGATTT+TGG + chr4.4:73894359-73894378 MS.gene48822:CDS 45.0%
! GACACGAATCGTTAGTGGAT+TGG + chr4.4:73894626-73894645 MS.gene48822:CDS 45.0%
! GCATCGATGGAAGAGACAAA+CGG + chr4.4:73894273-73894292 MS.gene48822:CDS 45.0%
! GGCAATTTTGCATCTTGCAG+CGG + chr4.4:73894698-73894717 MS.gene48822:CDS 45.0%
! GGTGATTTGGGAGTTAATGG+CGG + chr4.4:73895007-73895026 MS.gene48822:CDS 45.0%
! GTTGAGGATTTTACGTCGGT+GGG + chr4.4:73895067-73895086 MS.gene48822:CDS 45.0%
! TGTTGAGGATTTTACGTCGG+TGG + chr4.4:73895066-73895085 MS.gene48822:CDS 45.0%
! TTTTGCATCTTGCAGCGGAT+AGG + chr4.4:73894703-73894722 MS.gene48822:CDS 45.0%
!! CGGTGTTGTTTATGAGCTTC+GGG + chr4.4:73894293-73894312 MS.gene48822:CDS 45.0%
!! CGTTAGTGGATTGGTTGAGT+TGG + chr4.4:73894635-73894654 MS.gene48822:CDS 45.0%
!! CGTTCTTTTTGGTGCAACGA+CGG + chr4.4:73893852-73893871 MS.gene48822:CDS 45.0%
AATCCCTGCTGTCATCTCCA+CGG - chr4.4:73894760-73894779 None:intergenic 50.0%
AGGGATTATGGCGGTAATGC+CGG + chr4.4:73894773-73894792 MS.gene48822:CDS 50.0%
AGGTGGATCAGAGATTGTGG+CGG + chr4.4:73894956-73894975 MS.gene48822:CDS 50.0%
CGAGCTTGTATAGCTGAAGC+GGG + chr4.4:73894342-73894361 MS.gene48822:CDS 50.0%
CGGCAATTCATGGTGTGGAA+AGG + chr4.4:73894985-73895004 MS.gene48822:CDS 50.0%
GAAATGATGGCCGTTTACGG+CGG + chr4.4:73893884-73893903 MS.gene48822:CDS 50.0%
GATGACAGCAGGGATTATGG+CGG + chr4.4:73894764-73894783 MS.gene48822:CDS 50.0%
GATTTGGGAGTTAATGGCGG+TGG + chr4.4:73895010-73895029 MS.gene48822:CDS 50.0%
GCGGAATCAGTCGTTGGAAT+TGG + chr4.4:73893903-73893922 MS.gene48822:CDS 50.0%
GGAAGCAACAAGAATGACGG+CGG + chr4.4:73894209-73894228 MS.gene48822:CDS 50.0%
GGAGATGACAGCAGGGATTA+TGG + chr4.4:73894761-73894780 MS.gene48822:CDS 50.0%
GTTAAGGGAGGGATCAGAGA+GGG + chr4.4:73894890-73894909 MS.gene48822:CDS 50.0%
GTTGGCGAAAAATGGACCTG+AGG + chr4.4:73894653-73894672 MS.gene48822:CDS 50.0%
TATTAACTCCGTGTGGGCCA+GGG - chr4.4:73894073-73894092 None:intergenic 50.0%
TGTTAAGGGAGGGATCAGAG+AGG + chr4.4:73894889-73894908 MS.gene48822:CDS 50.0%
TTCGCAAAATCCGAGCCTAC+AGG + chr4.4:73894407-73894426 MS.gene48822:CDS 50.0%
TTTACGGCGGAATCAGTCGT+TGG + chr4.4:73893897-73893916 MS.gene48822:CDS 50.0%
TTTGGGAGTTAATGGCGGTG+GGG + chr4.4:73895012-73895031 MS.gene48822:CDS 50.0%
! ACGACTGATTCCGCCGTAAA+CGG - chr4.4:73893897-73893916 None:intergenic 50.0%
! GCCAGGGTTTGACCTGTTTT+AGG - chr4.4:73894057-73894076 None:intergenic 50.0%
! GGGTTTGGATGGTGGTGAAA+CGG + chr4.4:73895094-73895113 MS.gene48822:CDS 50.0%
! GTCGTTGGAATTGGTGGTTC+CGG + chr4.4:73893912-73893931 MS.gene48822:CDS 50.0%
! TTGGATGGTGGTGAAACGGA+GGG + chr4.4:73895098-73895117 MS.gene48822:CDS 50.0%
! TTTAAGATCGGGTGCCACGT+GGG + chr4.4:73894527-73894546 MS.gene48822:CDS 50.0%
! TTTGGATGGTGGTGAAACGG+AGG + chr4.4:73895097-73895116 MS.gene48822:CDS 50.0%
!! GGTTAAGCGTGGTGGATTAG+TGG + chr4.4:73894824-73894843 MS.gene48822:CDS 50.0%
!! TAAGCGTGGTGGATTAGTGG+CGG + chr4.4:73894827-73894846 MS.gene48822:CDS 50.0%
!! TGGGAAGGTTGGTAGAGAGT+GGG + chr4.4:73894733-73894752 MS.gene48822:CDS 50.0%
!!! TTTTAAGATCGGGTGCCACG+TGG + chr4.4:73894526-73894545 MS.gene48822:CDS 50.0%
ATTCGCTTTAGCCTCCCACG+TGG - chr4.4:73894544-73894563 None:intergenic 55.0%
CTTGAAGCCGTGGTTAAGCG+TGG + chr4.4:73894813-73894832 MS.gene48822:CDS 55.0%
GAGTGTTAGAGGGAGGAGGA+AGG + chr4.4:73895034-73895053 MS.gene48822:CDS 55.0%
GATTATGGCGGTAATGCCGG+AGG + chr4.4:73894776-73894795 MS.gene48822:CDS 55.0%
GCTTGCGGCAATTCATGGTG+TGG + chr4.4:73894980-73894999 MS.gene48822:CDS 55.0%
GGTGACGATACTTGAAGCCG+TGG + chr4.4:73894803-73894822 MS.gene48822:CDS 55.0%
GTATTAACTCCGTGTGGGCC+AGG - chr4.4:73894074-73894093 None:intergenic 55.0%
GTGGGAGGCTAAAGCGAATG+CGG + chr4.4:73894545-73894564 MS.gene48822:CDS 55.0%
TCTTGCAGCGGATAGGGAGA+CGG + chr4.4:73894710-73894729 MS.gene48822:CDS 55.0%
TGTTAGAGGGAGGAGGAAGG+CGG + chr4.4:73895037-73895056 MS.gene48822:CDS 55.0%
! AGAGATTGTGGCGGAGCTTG+CGG + chr4.4:73894965-73894984 MS.gene48822:CDS 55.0%
! ATTGGGGACGGTGTTAAGGG+AGG + chr4.4:73894878-73894897 MS.gene48822:CDS 55.0%
! GAGGATTTTACGTCGGTGGG+CGG + chr4.4:73895070-73895089 MS.gene48822:CDS 55.0%
! GTTGGAATTGGTGGTTCCGG+CGG + chr4.4:73893915-73893934 MS.gene48822:CDS 55.0%
! TTGGGGACGGTGTTAAGGGA+GGG + chr4.4:73894879-73894898 MS.gene48822:CDS 55.0%
!! ACGGCGTCGACAGGAAATGA+TGG + chr4.4:73893871-73893890 MS.gene48822:CDS 55.0%
!! GGGAAGGTTGGTAGAGAGTG+GGG + chr4.4:73894734-73894753 MS.gene48822:CDS 55.0%
!! GTGGGAAGGTTGGTAGAGAG+TGG + chr4.4:73894732-73894751 MS.gene48822:CDS 55.0%
!! TGGTGGATTAGTGGCGGTTG+CGG + chr4.4:73894833-73894852 MS.gene48822:CDS 55.0%
AGCGAAGAGGGACGCATTGG+CGG + chr4.4:73894677-73894696 MS.gene48822:CDS 60.0%
AGGTCAAACCCTGGCCCACA+CGG + chr4.4:73894062-73894081 MS.gene48822:CDS 60.0%
AGTATCGTCACCGCCTCCTC+CGG - chr4.4:73894795-73894814 None:intergenic 60.0%
ATCGGACACCGGTAATCCGC+CGG - chr4.4:73893934-73893953 None:intergenic 60.0%
ATGGACCTGAGGGAGCGAAG+AGG + chr4.4:73894664-73894683 MS.gene48822:CDS 60.0%
GAAGCCGTGGTTAAGCGTGG+TGG + chr4.4:73894816-73894835 MS.gene48822:CDS 60.0%
GATCCTGTCGTCGTGGCAAC+AGG + chr4.4:73893973-73893992 MS.gene48822:CDS 60.0%
GCGGAGCTTGCGGCAATTCA+TGG + chr4.4:73894975-73894994 MS.gene48822:CDS 60.0%
GGAGGCTAAAGCGAATGCGG+CGG + chr4.4:73894548-73894567 MS.gene48822:CDS 60.0%
TAGGGAGACGGTGGGAAGGT+TGG + chr4.4:73894722-73894741 MS.gene48822:CDS 60.0%
TATGGCGGTAATGCCGGAGG+AGG + chr4.4:73894779-73894798 MS.gene48822:CDS 60.0%
TGGACCTGAGGGAGCGAAGA+GGG + chr4.4:73894665-73894684 MS.gene48822:CDS 60.0%
TGTCCTGTTGCCACGACGAC+AGG - chr4.4:73893979-73893998 None:intergenic 60.0%
! ATTTTACGTCGGTGGGCGGC+GGG + chr4.4:73895074-73895093 MS.gene48822:CDS 60.0%
! CGGTTGCGGCGGCTTTTGTA+GGG + chr4.4:73894847-73894866 MS.gene48822:CDS 60.0%
! GATTTTACGTCGGTGGGCGG+CGG + chr4.4:73895073-73895092 MS.gene48822:CDS 60.0%
!! ATGGCGGTGGGGAGTGTTAG+AGG + chr4.4:73895023-73895042 MS.gene48822:CDS 60.0%
!! TGGATTAGTGGCGGTTGCGG+CGG + chr4.4:73894836-73894855 MS.gene48822:CDS 60.0%
!! TGGCGGTGGGGAGTGTTAGA+GGG + chr4.4:73895024-73895043 MS.gene48822:CDS 60.0%
!! TGGGGAGTGTTAGAGGGAGG+AGG + chr4.4:73895030-73895049 MS.gene48822:CDS 60.0%
!! TGGTGGTTCCGGCGGATTAC+CGG + chr4.4:73893923-73893942 MS.gene48822:CDS 60.0%
CGGATAGGGAGACGGTGGGA+AGG + chr4.4:73894718-73894737 MS.gene48822:CDS 65.0%
GATGCGGGATCCTGTCGTCG+TGG + chr4.4:73893966-73893985 MS.gene48822:CDS 65.0%
GCAGCGGATAGGGAGACGGT+GGG + chr4.4:73894714-73894733 MS.gene48822:CDS 65.0%
GGAAGGCGGCGACGTTGTTG+AGG + chr4.4:73895051-73895070 MS.gene48822:CDS 65.0%
GGAGGGATCAGAGAGGGCGA+GGG + chr4.4:73894896-73894915 MS.gene48822:CDS 65.0%
GGGAGCGAAGAGGGACGCAT+TGG + chr4.4:73894674-73894693 MS.gene48822:CDS 65.0%
GGGCCGTGGAGATGACAGCA+GGG + chr4.4:73894754-73894773 MS.gene48822:CDS 65.0%
TGCAGCGGATAGGGAGACGG+TGG + chr4.4:73894713-73894732 MS.gene48822:CDS 65.0%
! AAGATCGGGTGCCACGTGGG+AGG + chr4.4:73894530-73894549 MS.gene48822:CDS 65.0%
! GCGGTTGCGGCGGCTTTTGT+AGG + chr4.4:73894846-73894865 MS.gene48822:CDS 65.0%
! GTTGGTAGAGAGTGGGGCCG+TGG + chr4.4:73894740-73894759 MS.gene48822:CDS 65.0%
!! CGGTGGGGAGTGTTAGAGGG+AGG + chr4.4:73895027-73895046 MS.gene48822:CDS 65.0%
AGAGAGGGCGAGGGAGAGCG+CGG + chr4.4:73894905-73894924 MS.gene48822:CDS 70.0%
GCGTCCCTCTTCGCTCCCTC+AGG - chr4.4:73894672-73894691 None:intergenic 70.0%
GGCGGTAATGCCGGAGGAGG+CGG + chr4.4:73894782-73894801 MS.gene48822:CDS 70.0%
GGGAGGGATCAGAGAGGGCG+AGG + chr4.4:73894895-73894914 MS.gene48822:CDS 70.0%
GGGGCCGTGGAGATGACAGC+AGG + chr4.4:73894753-73894772 MS.gene48822:CDS 70.0%
! TGGGCGGCGGGTTTGGATGG+TGG + chr4.4:73895086-73895105 MS.gene48822:CDS 70.0%
!! GGTGCAACGACGGCGTCGAC+AGG + chr4.4:73893862-73893881 MS.gene48822:CDS 70.0%
! ACGTCGGTGGGCGGCGGGTT+TGG + chr4.4:73895079-73895098 MS.gene48822:CDS 75.0%
! CGGTGGGCGGCGGGTTTGGA+TGG + chr4.4:73895083-73895102 MS.gene48822:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 73893385 73895196 73893385 ID=MS.gene48822
chr4.4 mRNA 73893385 73895196 73893385 ID=MS.gene48822.t1;Parent=MS.gene48822
chr4.4 exon 73893385 73895196 73893385 ID=MS.gene48822.t1.exon1;Parent=MS.gene48822.t1
chr4.4 CDS 73893385 73895196 73893385 ID=cds.MS.gene48822.t1;Parent=MS.gene48822.t1
Gene Sequence

>MS.gene48822

ATGTATATTGTGCTTCATAGGATTAAAACTCTGCTGGAAGATTGTTCTAATGGAAGCAAATTTTCTATTCTTATGCAGATTGAAACTGTTGCTGATAATTTCAACAAATTAACAGGTGAATTATCAACTCTTCTTGATGTTTTTCCAATTCAAGAAATGAACTTGAATGATGATGTATTGGAGCTTGTTCTTCTGATTAGAAAACAGTGTTCAGAAACGAAGAGTTTGTTAGGTGAAGAACAAATTCATCTCCGAAATGATGTTGTTTCTGTTCTTGAAGGAATCAAAAAAGAAATTGTTCCGAAAGAGGAAAAATTATCTTCAATTTTTGAGAAATTGGAGATTTGTGATTCTTCGAGTTGTAGAAGTGAGATTGAAATTCTTCAGGAAGAAATTCAGAATCGGAACGAAGAACAATCGAAATCAGAGATTGTTGCGTTGATCGGACTTGTTCGTTATGCGAAGTGCGTTCTTTTTGGTGCAACGACGGCGTCGACAGGAAATGATGGCCGTTTACGGCGGAATCAGTCGTTGGAATTGGTGGTTCCGGCGGATTACCGGTGTCCGATAAGTCTTGAACTGATGCGGGATCCTGTCGTCGTGGCAACAGGACAGACGTACGATCGTGTTTCTATTAAGCTTTGGATGGATTCTGGGCACAACACGTGTCCTAAAACAGGTCAAACCCTGGCCCACACGGAGTTAATACCTAACCATGCTTTGAGAAATATGATAGCTATTTGGTGCAAAGAACAGAAAATTCCATTTGAAGGTGAAACGGTTGCCGTTAAAGTTAACGGTGGTATTACAAATAAGACAGCTTTGGAAGCAACAAGAATGACGGCGGCGTTTTTAGTCAACAAGTTGACGTTGACTAACGGTTCTTTAGCATCGATGGAAGAGACAAACGGTGTTGTTTATGAGCTTCGGGTTTTGTCGAAATCAGATTCGGAGAGTCGAGCTTGTATAGCTGAAGCGGGAGCTATAGAGATTTTGGTTCGGTTTTTAAGTTATGAAGTTGGTTCGCAAAATCCGAGCCTACAGGTTAATGCAGTTACAACGATATTAAATCTCTCGATACTTGAAGCGAATAAGACAAGGATAATGGAGACTGAAGGTGTTTTAAGTGGTGTAGCAGAGGTTTTAAGATCGGGTGCCACGTGGGAGGCTAAAGCGAATGCGGCGGCTACTGTGTTTAGTTTAACTGGAGTGGTTGCTTTTAGAAAGCGGTTAGGTAGGAAGACACGAATCGTTAGTGGATTGGTTGAGTTGGCGAAAAATGGACCTGAGGGAGCGAAGAGGGACGCATTGGCGGCAATTTTGCATCTTGCAGCGGATAGGGAGACGGTGGGAAGGTTGGTAGAGAGTGGGGCCGTGGAGATGACAGCAGGGATTATGGCGGTAATGCCGGAGGAGGCGGTGACGATACTTGAAGCCGTGGTTAAGCGTGGTGGATTAGTGGCGGTTGCGGCGGCTTTTGTAGGGATAAAAAAATTGGGGACGGTGTTAAGGGAGGGATCAGAGAGGGCGAGGGAGAGCGCGGCCGCGACACTTGTGACAATGTGTAGGAAAGGTGGATCAGAGATTGTGGCGGAGCTTGCGGCAATTCATGGTGTGGAAAGGGTGATTTGGGAGTTAATGGCGGTGGGGAGTGTTAGAGGGAGGAGGAAGGCGGCGACGTTGTTGAGGATTTTACGTCGGTGGGCGGCGGGTTTGGATGGTGGTGAAACGGAGGGATTTACATCAACAAACACTTTTGTTAGTACGTCAACTACCATGGTTGTTCCAACTACAACTTCATTAGCACAGTAA
Protein sequence

>MS.gene48822.t1

MYIVLHRIKTLLEDCSNGSKFSILMQIETVADNFNKLTGELSTLLDVFPIQEMNLNDDVLELVLLIRKQCSETKSLLGEEQIHLRNDVVSVLEGIKKEIVPKEEKLSSIFEKLEICDSSSCRSEIEILQEEIQNRNEEQSKSEIVALIGLVRYAKCVLFGATTASTGNDGRLRRNQSLELVVPADYRCPISLELMRDPVVVATGQTYDRVSIKLWMDSGHNTCPKTGQTLAHTELIPNHALRNMIAIWCKEQKIPFEGETVAVKVNGGITNKTALEATRMTAAFLVNKLTLTNGSLASMEETNGVVYELRVLSKSDSESRACIAEAGAIEILVRFLSYEVGSQNPSLQVNAVTTILNLSILEANKTRIMETEGVLSGVAEVLRSGATWEAKANAAATVFSLTGVVAFRKRLGRKTRIVSGLVELAKNGPEGAKRDALAAILHLAADRETVGRLVESGAVEMTAGIMAVMPEEAVTILEAVVKRGGLVAVAAAFVGIKKLGTVLREGSERARESAAATLVTMCRKGGSEIVAELAAIHGVERVIWELMAVGSVRGRRKAATLLRILRRWAAGLDGGETEGFTSTNTFVSTSTTMVVPTTTSLAQ