Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49404.t1 | XP_013468489.1 | 98.2 | 272 | 5 | 0 | 1 | 272 | 116 | 387 | 3.00E-152 | 547.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49404.t1 | Q9M0R2 | 46.7 | 261 | 132 | 3 | 16 | 272 | 326 | 583 | 1.6e-61 | 237.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49404.t1 | A0A072VLM7 | 98.2 | 272 | 5 | 0 | 1 | 272 | 116 | 387 | 2.1e-152 | 547.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene49404 | 0.806324 | 8.69E-50 | -1.69E-46 |
| MS.gene049396 | MS.gene49404 | 0.812416 | 4.29E-51 | -1.69E-46 |
| MS.gene053318 | MS.gene49404 | 0.809037 | 2.31E-50 | -1.69E-46 |
| MS.gene054486 | MS.gene49404 | 0.80388 | 2.82E-49 | -1.69E-46 |
| MS.gene055486 | MS.gene49404 | 0.82563 | 4.22E-54 | -1.69E-46 |
| MS.gene055909 | MS.gene49404 | 0.823596 | 1.27E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene49404 | 0.824255 | 8.91E-54 | -1.69E-46 |
| MS.gene05601 | MS.gene49404 | 0.804097 | 2.54E-49 | -1.69E-46 |
| MS.gene056166 | MS.gene49404 | 0.817393 | 3.38E-52 | -1.69E-46 |
| MS.gene056422 | MS.gene49404 | 0.81261 | 3.89E-51 | -1.69E-46 |
| MS.gene057734 | MS.gene49404 | 0.815233 | 1.03E-51 | -1.69E-46 |
| MS.gene059368 | MS.gene49404 | 0.832938 | 7.10E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene49404 | 0.845319 | 4.37E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATTGAGGAAATTCTTAA+AGG | 0.227165 | 1.1:+52079077 | MS.gene49404:CDS |
| ATCTTAGAGATATTACATTA+TGG | 0.248604 | 1.1:+52079010 | MS.gene49404:CDS |
| TAAAATTCTTGATGAAGATT+TGG | 0.250588 | 1.1:+52079176 | MS.gene49404:CDS |
| TGGTGTTGATGGAATTATTC+AGG | 0.274026 | 1.1:+52079395 | MS.gene49404:CDS |
| AGCTCTTTCATTCCTTGAAT+TGG | 0.286220 | 1.1:-52079438 | None:intergenic |
| TTGTAGTAAATGCACCATTT+TGG | 0.309402 | 1.1:+52079639 | MS.gene49404:CDS |
| AAAATTCTTGATGAAGATTT+GGG | 0.329182 | 1.1:+52079177 | MS.gene49404:CDS |
| GCTCAACTCCGTCAGCAAAA+AGG | 0.352272 | 1.1:+52079458 | MS.gene49404:CDS |
| AACACTCACCTGAGAAGAAT+TGG | 0.366039 | 1.1:-52079749 | None:intergenic |
| TCTTAGAGATATTACATTAT+GGG | 0.371202 | 1.1:+52079011 | MS.gene49404:CDS |
| TGTGCCCTACTTGGTAATAA+AGG | 0.371867 | 1.1:-52079039 | None:intergenic |
| GAGATGGAGGATTCAATTGA+TGG | 0.376525 | 1.1:+52079341 | MS.gene49404:CDS |
| AATTTGTACAAGAAGACATT+TGG | 0.376644 | 1.1:+52079291 | MS.gene49404:CDS |
| GAGCATCTTCCAGCTGGATA+TGG | 0.380972 | 1.1:+52079863 | MS.gene49404:CDS |
| CAGCTGGATATGGTGGTCTA+AGG | 0.408968 | 1.1:+52079873 | MS.gene49404:CDS |
| TATCCAGCTGGAAGATGCTC+TGG | 0.411235 | 1.1:-52079860 | None:intergenic |
| TTGAAAAGTGCACCAATTCA+AGG | 0.425968 | 1.1:+52079426 | MS.gene49404:CDS |
| TGTGAAGTGTAGAGCCAAAA+TGG | 0.434368 | 1.1:-52079653 | None:intergenic |
| TGTTACAGAAGACATTGGTT+TGG | 0.442089 | 1.1:+52079133 | MS.gene49404:CDS |
| TGCTCTAAAACTTTATCATC+AGG | 0.448465 | 1.1:-52079920 | None:intergenic |
| AGAATTGGTGTGACCCTTTG+TGG | 0.480253 | 1.1:-52079734 | None:intergenic |
| GGCCCAGAGCATCTTCCAGC+TGG | 0.482220 | 1.1:+52079857 | MS.gene49404:CDS |
| GTACCTTCATGTGCCCTACT+TGG | 0.493972 | 1.1:-52079048 | None:intergenic |
| ATATTTGGATAGTAGAGATA+AGG | 0.496144 | 1.1:+52079221 | MS.gene49404:CDS |
| TTGTTGAGCACCTCATTGAC+TGG | 0.499653 | 1.1:-52079980 | None:intergenic |
| GTGTTCCTTTATTACCAAGT+AGG | 0.502140 | 1.1:+52079034 | MS.gene49404:CDS |
| TGACCCTTTGTGGTCTTGCA+AGG | 0.502712 | 1.1:-52079724 | None:intergenic |
| TGTTCCTTTATTACCAAGTA+GGG | 0.503280 | 1.1:+52079035 | MS.gene49404:CDS |
| GGGTCACACCAATTCTTCTC+AGG | 0.506747 | 1.1:+52079741 | MS.gene49404:CDS |
| CATCCTTGCAAGACCACAAA+GGG | 0.520308 | 1.1:+52079721 | MS.gene49404:CDS |
| CTTCACAAGTGTTATTCTCA+AGG | 0.523067 | 1.1:+52079669 | MS.gene49404:CDS |
| ATGAAATGTCACAGGTACAT+AGG | 0.530202 | 1.1:+52079836 | MS.gene49404:intron |
| CTTAGAGATATTACATTATG+GGG | 0.542377 | 1.1:+52079012 | MS.gene49404:CDS |
| TCGTAAACATAGTAACAAAC+CGG | 0.557690 | 1.1:-52079252 | None:intergenic |
| TAGAGATAAGGAAGGTCGAC+CGG | 0.565192 | 1.1:+52079233 | MS.gene49404:CDS |
| CTTAGACCACCATATCCAGC+TGG | 0.567452 | 1.1:-52079872 | None:intergenic |
| CATCTTCCAGCTGGATATGG+TGG | 0.574311 | 1.1:+52079866 | MS.gene49404:CDS |
| ATCCAGCTGGAAGATGCTCT+GGG | 0.577545 | 1.1:-52079859 | None:intergenic |
| GTTGTTTCAAAGCTATTACC+CGG | 0.594800 | 1.1:+52079488 | MS.gene49404:CDS |
| TGACATGTTACAGAAGACAT+TGG | 0.594854 | 1.1:+52079128 | MS.gene49404:CDS |
| CTGGATATGGTGGTCTAAGG+AGG | 0.598272 | 1.1:+52079876 | MS.gene49404:CDS |
| AGTTGAATTTCCAGTCAATG+AGG | 0.619748 | 1.1:+52079970 | MS.gene49404:CDS |
| TTGGATAGTAGAGATAAGGA+AGG | 0.628322 | 1.1:+52079225 | MS.gene49404:CDS |
| TTACCAAGTAGGGCACATGA+AGG | 0.629064 | 1.1:+52079045 | MS.gene49404:CDS |
| AAGGTACTGATATTGTATTG+AGG | 0.652952 | 1.1:+52079064 | MS.gene49404:CDS |
| TCATCCTTGCAAGACCACAA+AGG | 0.655234 | 1.1:+52079720 | MS.gene49404:CDS |
| GGTTTGTTACTATGTTTACG+AGG | 0.673551 | 1.1:+52079254 | MS.gene49404:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCTTGATGAAGATTT+GGG | + | chr1.1:52079177-52079196 | MS.gene49404:CDS | 20.0% |
| !! | ATCTTAGAGATATTACATTA+TGG | + | chr1.1:52079010-52079029 | MS.gene49404:CDS | 20.0% |
| !! | TAAAATTCTTGATGAAGATT+TGG | + | chr1.1:52079176-52079195 | MS.gene49404:CDS | 20.0% |
| !! | TCTTAGAGATATTACATTAT+GGG | + | chr1.1:52079011-52079030 | MS.gene49404:CDS | 20.0% |
| !! | TTCATATATACTATAAGCTA+TGG | - | chr1.1:52079606-52079625 | None:intergenic | 20.0% |
| !!! | ACATTTTTATAAACTATCTC+CGG | - | chr1.1:52079510-52079529 | None:intergenic | 20.0% |
| !!! | TTTTGAAAAAGTTGGATATT+TGG | + | chr1.1:52079206-52079225 | MS.gene49404:CDS | 20.0% |
| ! | AATTTGTACAAGAAGACATT+TGG | + | chr1.1:52079291-52079310 | MS.gene49404:CDS | 25.0% |
| ! | AGAAAAAATTTCATTGCCAA+AGG | + | chr1.1:52078984-52079003 | MS.gene49404:CDS | 25.0% |
| ! | ATATTTGGATAGTAGAGATA+AGG | + | chr1.1:52079221-52079240 | MS.gene49404:CDS | 25.0% |
| ! | CTTAGAGATATTACATTATG+GGG | + | chr1.1:52079012-52079031 | MS.gene49404:CDS | 25.0% |
| ! | TGTATTGAGGAAATTCTTAA+AGG | + | chr1.1:52079077-52079096 | MS.gene49404:CDS | 25.0% |
| ! | TTAACATTATGAAATGTCAC+AGG | + | chr1.1:52079828-52079847 | MS.gene49404:intron | 25.0% |
| !! | CATTTTTATAAACTATCTCC+GGG | - | chr1.1:52079509-52079528 | None:intergenic | 25.0% |
| !!! | ATATCTCTAAGATTTTCCTT+TGG | - | chr1.1:52079003-52079022 | None:intergenic | 25.0% |
| !!! | ATTAAGAAGTTGAGTTTTAG+AGG | + | chr1.1:52079372-52079391 | MS.gene49404:CDS | 25.0% |
| TATCATCAGGAGAAAAATCT+TGG | - | chr1.1:52079910-52079929 | None:intergenic | 30.0% | |
| TCGTAAACATAGTAACAAAC+CGG | - | chr1.1:52079255-52079274 | None:intergenic | 30.0% | |
| TGTTCCTTTATTACCAAGTA+GGG | + | chr1.1:52079035-52079054 | MS.gene49404:CDS | 30.0% | |
| ! | TTGTAGTAAATGCACCATTT+TGG | + | chr1.1:52079639-52079658 | MS.gene49404:CDS | 30.0% |
| !! | AAGGTACTGATATTGTATTG+AGG | + | chr1.1:52079064-52079083 | MS.gene49404:CDS | 30.0% |
| !! | GGTTCTGATTTTGAAAAAGT+TGG | + | chr1.1:52079198-52079217 | MS.gene49404:CDS | 30.0% |
| !! | TGAACTTCTTTTTGTTTCTC+TGG | - | chr1.1:52079703-52079722 | None:intergenic | 30.0% |
| !! | TGCTCTAAAACTTTATCATC+AGG | - | chr1.1:52079923-52079942 | None:intergenic | 30.0% |
| !!! | AATGTGACTTGTTTTTGAGA+TGG | + | chr1.1:52079325-52079344 | MS.gene49404:CDS | 30.0% |
| AGCTCTTTCATTCCTTGAAT+TGG | - | chr1.1:52079441-52079460 | None:intergenic | 35.0% | |
| AGTTGAATTTCCAGTCAATG+AGG | + | chr1.1:52079970-52079989 | MS.gene49404:CDS | 35.0% | |
| ATGAAATGTCACAGGTACAT+AGG | + | chr1.1:52079836-52079855 | MS.gene49404:intron | 35.0% | |
| GTGTTCCTTTATTACCAAGT+AGG | + | chr1.1:52079034-52079053 | MS.gene49404:CDS | 35.0% | |
| TGACATGTTACAGAAGACAT+TGG | + | chr1.1:52079128-52079147 | MS.gene49404:CDS | 35.0% | |
| TTGGATAGTAGAGATAAGGA+AGG | + | chr1.1:52079225-52079244 | MS.gene49404:CDS | 35.0% | |
| ! | CTTCACAAGTGTTATTCTCA+AGG | + | chr1.1:52079669-52079688 | MS.gene49404:CDS | 35.0% |
| ! | GTTGTTTCAAAGCTATTACC+CGG | + | chr1.1:52079488-52079507 | MS.gene49404:CDS | 35.0% |
| ! | TGTTACAGAAGACATTGGTT+TGG | + | chr1.1:52079133-52079152 | MS.gene49404:CDS | 35.0% |
| ! | TTGAAAAGTGCACCAATTCA+AGG | + | chr1.1:52079426-52079445 | MS.gene49404:CDS | 35.0% |
| !! | GGTTTGTTACTATGTTTACG+AGG | + | chr1.1:52079254-52079273 | MS.gene49404:CDS | 35.0% |
| !! | TGGTGTTGATGGAATTATTC+AGG | + | chr1.1:52079395-52079414 | MS.gene49404:CDS | 35.0% |
| !!! | AAGAAGTTGAGTTTTAGAGG+TGG | + | chr1.1:52079375-52079394 | MS.gene49404:CDS | 35.0% |
| AACACTCACCTGAGAAGAAT+TGG | - | chr1.1:52079752-52079771 | None:intergenic | 40.0% | |
| GAGATGGAGGATTCAATTGA+TGG | + | chr1.1:52079341-52079360 | MS.gene49404:CDS | 40.0% | |
| TGTGAAGTGTAGAGCCAAAA+TGG | - | chr1.1:52079656-52079675 | None:intergenic | 40.0% | |
| ! | CATAAGAGCCTTTTTGCTGA+CGG | - | chr1.1:52079469-52079488 | None:intergenic | 40.0% |
| ! | TGTGCCCTACTTGGTAATAA+AGG | - | chr1.1:52079042-52079061 | None:intergenic | 40.0% |
| !!! | AGTTTTAGAGGTGGTGTTGA+TGG | + | chr1.1:52079384-52079403 | MS.gene49404:CDS | 40.0% |
| !!! | GTGACTTGTTTTTGAGATGG+AGG | + | chr1.1:52079328-52079347 | MS.gene49404:CDS | 40.0% |
| AGAATTGGTGTGACCCTTTG+TGG | - | chr1.1:52079737-52079756 | None:intergenic | 45.0% | |
| CATCCTTGCAAGACCACAAA+GGG | + | chr1.1:52079721-52079740 | MS.gene49404:CDS | 45.0% | |
| TAGAGATAAGGAAGGTCGAC+CGG | + | chr1.1:52079233-52079252 | MS.gene49404:CDS | 45.0% | |
| TCATCCTTGCAAGACCACAA+AGG | + | chr1.1:52079720-52079739 | MS.gene49404:CDS | 45.0% | |
| TTACCAAGTAGGGCACATGA+AGG | + | chr1.1:52079045-52079064 | MS.gene49404:CDS | 45.0% | |
| ATCCAGCTGGAAGATGCTCT+GGG | - | chr1.1:52079862-52079881 | None:intergenic | 50.0% | |
| CAGCTGGATATGGTGGTCTA+AGG | + | chr1.1:52079873-52079892 | MS.gene49404:CDS | 50.0% | |
| CATCTTCCAGCTGGATATGG+TGG | + | chr1.1:52079866-52079885 | MS.gene49404:CDS | 50.0% | |
| CTGGATATGGTGGTCTAAGG+AGG | + | chr1.1:52079876-52079895 | MS.gene49404:CDS | 50.0% | |
| CTTAGACCACCATATCCAGC+TGG | - | chr1.1:52079875-52079894 | None:intergenic | 50.0% | |
| GAGCATCTTCCAGCTGGATA+TGG | + | chr1.1:52079863-52079882 | MS.gene49404:CDS | 50.0% | |
| GCTCAACTCCGTCAGCAAAA+AGG | + | chr1.1:52079458-52079477 | MS.gene49404:CDS | 50.0% | |
| GGGTCACACCAATTCTTCTC+AGG | + | chr1.1:52079741-52079760 | MS.gene49404:CDS | 50.0% | |
| GTACCTTCATGTGCCCTACT+TGG | - | chr1.1:52079051-52079070 | None:intergenic | 50.0% | |
| TATCCAGCTGGAAGATGCTC+TGG | - | chr1.1:52079863-52079882 | None:intergenic | 50.0% | |
| ! | TGACCCTTTGTGGTCTTGCA+AGG | - | chr1.1:52079727-52079746 | None:intergenic | 50.0% |
| ! | GGCCCAGAGCATCTTCCAGC+TGG | + | chr1.1:52079857-52079876 | MS.gene49404:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 52078977 | 52079991 | 52078977 | ID=MS.gene49404 |
| chr1.1 | mRNA | 52078977 | 52079991 | 52078977 | ID=MS.gene49404.t1;Parent=MS.gene49404 |
| chr1.1 | exon | 52078977 | 52079527 | 52078977 | ID=MS.gene49404.t1.exon1;Parent=MS.gene49404.t1 |
| chr1.1 | CDS | 52078977 | 52079527 | 52078977 | ID=cds.MS.gene49404.t1;Parent=MS.gene49404.t1 |
| chr1.1 | exon | 52079638 | 52079762 | 52079638 | ID=MS.gene49404.t1.exon2;Parent=MS.gene49404.t1 |
| chr1.1 | CDS | 52079638 | 52079762 | 52079638 | ID=cds.MS.gene49404.t1;Parent=MS.gene49404.t1 |
| chr1.1 | exon | 52079850 | 52079991 | 52079850 | ID=MS.gene49404.t1.exon3;Parent=MS.gene49404.t1 |
| chr1.1 | CDS | 52079850 | 52079991 | 52079850 | ID=cds.MS.gene49404.t1;Parent=MS.gene49404.t1 |
| Gene Sequence |
| Protein sequence |
