Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51143.t1 | XP_003624198.2 | 98.6 | 209 | 3 | 0 | 1 | 209 | 1 | 209 | 1.80E-109 | 405.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51143.t1 | O82251 | 53.1 | 213 | 91 | 3 | 1 | 209 | 1 | 208 | 3.3e-51 | 203.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51143.t1 | G7KRK7 | 98.6 | 209 | 3 | 0 | 1 | 209 | 1 | 209 | 1.3e-109 | 405.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050876 | MS.gene51143 | 0.812744 | 3.63E-51 | -1.69E-46 |
| MS.gene051538 | MS.gene51143 | -0.800183 | 1.62E-48 | -1.69E-46 |
| MS.gene052570 | MS.gene51143 | -0.850825 | 1.32E-60 | -1.69E-46 |
| MS.gene052579 | MS.gene51143 | -0.814868 | 1.24E-51 | -1.69E-46 |
| MS.gene055204 | MS.gene51143 | -0.822075 | 2.88E-53 | -1.69E-46 |
| MS.gene055339 | MS.gene51143 | -0.809661 | 1.69E-50 | -1.69E-46 |
| MS.gene055506 | MS.gene51143 | -0.820928 | 5.29E-53 | -1.69E-46 |
| MS.gene056405 | MS.gene51143 | 0.824623 | 7.30E-54 | -1.69E-46 |
| MS.gene056607 | MS.gene51143 | 0.804304 | 2.30E-49 | -1.69E-46 |
| MS.gene057148 | MS.gene51143 | 0.842169 | 3.05E-58 | -1.69E-46 |
| MS.gene059026 | MS.gene51143 | 0.826823 | 2.20E-54 | -1.69E-46 |
| MS.gene05985 | MS.gene51143 | 0.803722 | 3.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51143.t1 | MTR_7g080300 | 98.565 | 209 | 3 | 0 | 1 | 209 | 1 | 209 | 2.16e-148 | 410 |
| MS.gene51143.t1 | MTR_5g095770 | 41.237 | 194 | 110 | 3 | 18 | 209 | 23 | 214 | 5.42e-44 | 146 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51143.t1 | AT2G47840 | 53.052 | 213 | 91 | 3 | 1 | 209 | 1 | 208 | 5.65e-69 | 210 |
| MS.gene51143.t1 | AT5G55710 | 43.396 | 159 | 88 | 2 | 51 | 209 | 52 | 208 | 1.81e-39 | 134 |
Find 54 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGTTAGGGTTGAGGATT+TGG | 0.168614 | 7.3:+27226742 | None:intergenic |
| AGGGTTAGGGTTGAGGATTT+GGG | 0.198298 | 7.3:+27226743 | None:intergenic |
| CGCGTTCTTCGCGCTTTACT+TGG | 0.229159 | 7.3:-27226453 | MS.gene51143:CDS |
| TAGTGCTTTGTGTTGTCTTT+TGG | 0.245663 | 7.3:-27226237 | MS.gene51143:CDS |
| TTTGAGTGATGGTGGGGTTA+AGG | 0.270194 | 7.3:+27226688 | None:intergenic |
| AATGAAAGGGAATTGGGTTT+TGG | 0.297041 | 7.3:+27226716 | None:intergenic |
| TCGAAGAGGAGTGCGACTTT+AGG | 0.305665 | 7.3:+27226530 | None:intergenic |
| CGAAGAGGAGTGCGACTTTA+GGG | 0.326657 | 7.3:+27226531 | None:intergenic |
| GGGTTAGGGTTGAGGATTTG+GGG | 0.333730 | 7.3:+27226744 | None:intergenic |
| TTGAGTGATGGTGGGGTTAA+GGG | 0.345958 | 7.3:+27226689 | None:intergenic |
| AAAGAGAGGAAAGGGATGAT+TGG | 0.362932 | 7.3:+27226506 | None:intergenic |
| GGGTGAGAATGAAAGGGAAT+TGG | 0.363246 | 7.3:+27226709 | None:intergenic |
| TCTGACATTAGATGTGCTTC+TGG | 0.365125 | 7.3:-27226375 | MS.gene51143:CDS |
| TCTCCTGGGAGATATGGATT+AGG | 0.379357 | 7.3:-27226323 | MS.gene51143:CDS |
| ATCTATAGAAAGAGAGGAAA+GGG | 0.392520 | 7.3:+27226498 | None:intergenic |
| GGTGAGAATGAAAGGGAATT+GGG | 0.412380 | 7.3:+27226710 | None:intergenic |
| GCTATTGAGATTAACCGTTC+TGG | 0.416946 | 7.3:+27226614 | None:intergenic |
| CCGTTCTGGTGCAGCGGGTG+TGG | 0.425454 | 7.3:+27226628 | None:intergenic |
| AAGAGAGGAAAGGGATGATT+GGG | 0.445801 | 7.3:+27226507 | None:intergenic |
| TGCAGCGGGTGTGGATGTGC+TGG | 0.452850 | 7.3:+27226637 | None:intergenic |
| TCAGCAACAAAAGGCAAGTA+AGG | 0.454318 | 7.3:+27226206 | None:intergenic |
| TTCTTCAATTCCCTTCAATA+CGG | 0.460547 | 7.3:-27226575 | MS.gene51143:CDS |
| GGTTAGGGTTGAGGATTTGG+GGG | 0.473050 | 7.3:+27226745 | None:intergenic |
| GCGTTCTTCGCGCTTTACTT+GGG | 0.478065 | 7.3:-27226452 | MS.gene51143:CDS |
| CTGAGTTTGAGTTTGAGTGA+TGG | 0.484633 | 7.3:+27226677 | None:intergenic |
| GGGTTAAGGGTGAGAATGAA+AGG | 0.486254 | 7.3:+27226702 | None:intergenic |
| CGCTTTACTTGGGAATCGTA+AGG | 0.497431 | 7.3:-27226442 | MS.gene51143:CDS |
| AGTTTGAGTTTGAGTGATGG+TGG | 0.499290 | 7.3:+27226680 | None:intergenic |
| CTCAAACTCAAACTCAGAAA+CGG | 0.504515 | 7.3:-27226671 | MS.gene51143:CDS |
| ATTAACCGTTCTGGTGCAGC+GGG | 0.508710 | 7.3:+27226623 | None:intergenic |
| GGAGGGATCTATAGAAAGAG+AGG | 0.516024 | 7.3:+27226492 | None:intergenic |
| GTTTGAGTTTGAGTGATGGT+GGG | 0.521464 | 7.3:+27226681 | None:intergenic |
| GCAGCAGCATCAGCAACAAA+AGG | 0.524058 | 7.3:+27226197 | None:intergenic |
| AACGCGATGAACGACGCGTA+AGG | 0.529024 | 7.3:+27226470 | None:intergenic |
| GATCTATAGAAAGAGAGGAA+AGG | 0.539602 | 7.3:+27226497 | None:intergenic |
| TTCTTCCGATGCTGTTTCAG+AGG | 0.540592 | 7.3:-27226352 | MS.gene51143:CDS |
| TTTGAGTTTGAGTGATGGTG+GGG | 0.542463 | 7.3:+27226682 | None:intergenic |
| TGTTAGATTCAATGCTATGC+AGG | 0.547502 | 7.3:-27226399 | MS.gene51143:CDS |
| TATTGAAGGGAATTGAAGAA+TGG | 0.550473 | 7.3:+27226578 | None:intergenic |
| GAATGTTGTGACCGTATTGA+AGG | 0.554113 | 7.3:+27226564 | None:intergenic |
| AAGGGATGATTGGGTCGAAG+AGG | 0.560538 | 7.3:+27226516 | None:intergenic |
| AGGGACGGTGGAGAAGAGAA+AGG | 0.566596 | 7.3:+27226783 | None:intergenic |
| GGGACGGTGGAGAAGAGAAA+GGG | 0.566728 | 7.3:+27226784 | None:intergenic |
| ACGCGATGAACGACGCGTAA+GGG | 0.572097 | 7.3:+27226471 | None:intergenic |
| GATTAACCGTTCTGGTGCAG+CGG | 0.578745 | 7.3:+27226622 | None:intergenic |
| AAACCTAATCCATATCTCCC+AGG | 0.588089 | 7.3:+27226320 | None:intergenic |
| AAAATCCTCTGAAACAGCAT+CGG | 0.612184 | 7.3:+27226347 | None:intergenic |
| CGATGAACGACGCGTAAGGG+AGG | 0.636592 | 7.3:+27226474 | None:intergenic |
| GATGAACGACGCGTAAGGGA+GGG | 0.642182 | 7.3:+27226475 | None:intergenic |
| GGTTAAGGGTGAGAATGAAA+GGG | 0.649568 | 7.3:+27226703 | None:intergenic |
| GAAGAATGGAAGTGAGTATG+CGG | 0.658731 | 7.3:+27226592 | None:intergenic |
| AATGTTGTGACCGTATTGAA+GGG | 0.659290 | 7.3:+27226565 | None:intergenic |
| CCACACCCGCTGCACCAGAA+CGG | 0.664416 | 7.3:-27226628 | MS.gene51143:CDS |
| GCATTGAATCTAACATACTG+CGG | 0.705676 | 7.3:+27226407 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAGGTTTTAAGATTTTGGTT+TGG | - | chr7.3:27226671-27226690 | MS.gene51143:CDS | 25.0% |
| !!! | TGGATTAGGTTTTAAGATTT+TGG | - | chr7.3:27226666-27226685 | MS.gene51143:CDS | 25.0% |
| ATCTATAGAAAGAGAGGAAA+GGG | + | chr7.3:27226480-27226499 | None:intergenic | 30.0% | |
| TATTGAAGGGAATTGAAGAA+TGG | + | chr7.3:27226400-27226419 | None:intergenic | 30.0% | |
| TTCTTCAATTCCCTTCAATA+CGG | - | chr7.3:27226400-27226419 | MS.gene51143:CDS | 30.0% | |
| AATGTTGTGACCGTATTGAA+GGG | + | chr7.3:27226413-27226432 | None:intergenic | 35.0% | |
| CTAACATACTGCGGAAAAAT+GGG | + | chr7.3:27226562-27226581 | None:intergenic | 35.0% | |
| CTCAAACTCAAACTCAGAAA+CGG | - | chr7.3:27226304-27226323 | MS.gene51143:CDS | 35.0% | |
| GATCTATAGAAAGAGAGGAA+AGG | + | chr7.3:27226481-27226500 | None:intergenic | 35.0% | |
| GCATTGAATCTAACATACTG+CGG | + | chr7.3:27226571-27226590 | None:intergenic | 35.0% | |
| TAACATACTGCGGAAAAATG+GGG | + | chr7.3:27226561-27226580 | None:intergenic | 35.0% | |
| TCTAACATACTGCGGAAAAA+TGG | + | chr7.3:27226563-27226582 | None:intergenic | 35.0% | |
| TGTTAGATTCAATGCTATGC+AGG | - | chr7.3:27226576-27226595 | MS.gene51143:CDS | 35.0% | |
| ! | AATGAAAGGGAATTGGGTTT+TGG | + | chr7.3:27226262-27226281 | None:intergenic | 35.0% |
| ! | AGTGCTTTGTGTTGTCTTTT+GGG | - | chr7.3:27226739-27226758 | MS.gene51143:CDS | 35.0% |
| ! | TAGTGCTTTGTGTTGTCTTT+TGG | - | chr7.3:27226738-27226757 | MS.gene51143:CDS | 35.0% |
| !! | AAAATCCTCTGAAACAGCAT+CGG | + | chr7.3:27226631-27226650 | None:intergenic | 35.0% |
| !!! | GGAATTGGGTTTTGGTTTTA+GGG | + | chr7.3:27226254-27226273 | None:intergenic | 35.0% |
| AAACCTAATCCATATCTCCC+AGG | + | chr7.3:27226658-27226677 | None:intergenic | 40.0% | |
| AAAGAGAGGAAAGGGATGAT+TGG | + | chr7.3:27226472-27226491 | None:intergenic | 40.0% | |
| AAGAGAGGAAAGGGATGATT+GGG | + | chr7.3:27226471-27226490 | None:intergenic | 40.0% | |
| GAAGAATGGAAGTGAGTATG+CGG | + | chr7.3:27226386-27226405 | None:intergenic | 40.0% | |
| GAATGTTGTGACCGTATTGA+AGG | + | chr7.3:27226414-27226433 | None:intergenic | 40.0% | |
| GCTATTGAGATTAACCGTTC+TGG | + | chr7.3:27226364-27226383 | None:intergenic | 40.0% | |
| GGTGAGAATGAAAGGGAATT+GGG | + | chr7.3:27226268-27226287 | None:intergenic | 40.0% | |
| GGTTAAGGGTGAGAATGAAA+GGG | + | chr7.3:27226275-27226294 | None:intergenic | 40.0% | |
| TCAGCAACAAAAGGCAAGTA+AGG | + | chr7.3:27226772-27226791 | None:intergenic | 40.0% | |
| TCTGACATTAGATGTGCTTC+TGG | - | chr7.3:27226600-27226619 | MS.gene51143:CDS | 40.0% | |
| ! | AGTTTGAGTTTGAGTGATGG+TGG | + | chr7.3:27226298-27226317 | None:intergenic | 40.0% |
| ! | ATTTTCTCTCCTGGGAGATA+TGG | - | chr7.3:27226646-27226665 | MS.gene51143:CDS | 40.0% |
| ! | CTGAGTTTGAGTTTGAGTGA+TGG | + | chr7.3:27226301-27226320 | None:intergenic | 40.0% |
| ! | GTTTGAGTTTGAGTGATGGT+GGG | + | chr7.3:27226297-27226316 | None:intergenic | 40.0% |
| ! | TTCAGAGGATTTTCTCTCCT+GGG | - | chr7.3:27226638-27226657 | MS.gene51143:CDS | 40.0% |
| ! | TTTCAGAGGATTTTCTCTCC+TGG | - | chr7.3:27226637-27226656 | MS.gene51143:CDS | 40.0% |
| ! | TTTGAGTTTGAGTGATGGTG+GGG | + | chr7.3:27226296-27226315 | None:intergenic | 40.0% |
| !! | CTTTGTGTTGTCTTTTGGGT+AGG | - | chr7.3:27226743-27226762 | MS.gene51143:CDS | 40.0% |
| !!! | GGGAATTGGGTTTTGGTTTT+AGG | + | chr7.3:27226255-27226274 | None:intergenic | 40.0% |
| !!! | GGGTTTTGGTTTTAGGGTTA+GGG | + | chr7.3:27226248-27226267 | None:intergenic | 40.0% |
| !!! | TGGGTTTTGGTTTTAGGGTT+AGG | + | chr7.3:27226249-27226268 | None:intergenic | 40.0% |
| AGGGTTAGGGTTGAGGATTT+GGG | + | chr7.3:27226235-27226254 | None:intergenic | 45.0% | |
| CGCTTTACTTGGGAATCGTA+AGG | - | chr7.3:27226533-27226552 | MS.gene51143:CDS | 45.0% | |
| GGAGGGATCTATAGAAAGAG+AGG | + | chr7.3:27226486-27226505 | None:intergenic | 45.0% | |
| GGGTGAGAATGAAAGGGAAT+TGG | + | chr7.3:27226269-27226288 | None:intergenic | 45.0% | |
| GGGTTAAGGGTGAGAATGAA+AGG | + | chr7.3:27226276-27226295 | None:intergenic | 45.0% | |
| TAGGGTTAGGGTTGAGGATT+TGG | + | chr7.3:27226236-27226255 | None:intergenic | 45.0% | |
| TCTCCTGGGAGATATGGATT+AGG | - | chr7.3:27226652-27226671 | MS.gene51143:CDS | 45.0% | |
| TGTGGTGAAAAATGCAGGGA+CGG | + | chr7.3:27226210-27226229 | None:intergenic | 45.0% | |
| !! | TTCTTCCGATGCTGTTTCAG+AGG | - | chr7.3:27226623-27226642 | MS.gene51143:CDS | 45.0% |
| !! | TTGAGTGATGGTGGGGTTAA+GGG | + | chr7.3:27226289-27226308 | None:intergenic | 45.0% |
| !! | TTTGAGTGATGGTGGGGTTA+AGG | + | chr7.3:27226290-27226309 | None:intergenic | 45.0% |
| !!! | TGGTTTTAGGGTTAGGGTTG+AGG | + | chr7.3:27226242-27226261 | None:intergenic | 45.0% |
| AAGGGATGATTGGGTCGAAG+AGG | + | chr7.3:27226462-27226481 | None:intergenic | 50.0% | |
| ATTAACCGTTCTGGTGCAGC+GGG | + | chr7.3:27226355-27226374 | None:intergenic | 50.0% | |
| GATTAACCGTTCTGGTGCAG+CGG | + | chr7.3:27226356-27226375 | None:intergenic | 50.0% | |
| GCAGCAGCATCAGCAACAAA+AGG | + | chr7.3:27226781-27226800 | None:intergenic | 50.0% | |
| GCGTTCTTCGCGCTTTACTT+GGG | - | chr7.3:27226523-27226542 | MS.gene51143:CDS | 50.0% | |
| GGGGTGTGGTGAAAAATGCA+GGG | + | chr7.3:27226214-27226233 | None:intergenic | 50.0% | |
| GGGTTAGGGTTGAGGATTTG+GGG | + | chr7.3:27226234-27226253 | None:intergenic | 50.0% | |
| GGTTAGGGTTGAGGATTTGG+GGG | + | chr7.3:27226233-27226252 | None:intergenic | 50.0% | |
| ! | CGAAGAGGAGTGCGACTTTA+GGG | + | chr7.3:27226447-27226466 | None:intergenic | 50.0% |
| ! | TCGAAGAGGAGTGCGACTTT+AGG | + | chr7.3:27226448-27226467 | None:intergenic | 50.0% |
| AACGCGATGAACGACGCGTA+AGG | + | chr7.3:27226508-27226527 | None:intergenic | 55.0% | |
| ACGCGATGAACGACGCGTAA+GGG | + | chr7.3:27226507-27226526 | None:intergenic | 55.0% | |
| AGGGACGGTGGAGAAGAGAA+AGG | + | chr7.3:27226195-27226214 | None:intergenic | 55.0% | |
| CGCGTTCTTCGCGCTTTACT+TGG | - | chr7.3:27226522-27226541 | MS.gene51143:CDS | 55.0% | |
| GATGAACGACGCGTAAGGGA+GGG | + | chr7.3:27226503-27226522 | None:intergenic | 55.0% | |
| GGGACGGTGGAGAAGAGAAA+GGG | + | chr7.3:27226194-27226213 | None:intergenic | 55.0% | |
| GGTGAAAAATGCAGGGACGG+TGG | + | chr7.3:27226207-27226226 | None:intergenic | 55.0% | |
| ! | GGGGGTGTGGTGAAAAATGC+AGG | + | chr7.3:27226215-27226234 | None:intergenic | 55.0% |
| CGATGAACGACGCGTAAGGG+AGG | + | chr7.3:27226504-27226523 | None:intergenic | 60.0% | |
| GGGTTGAGGATTTGGGGGTG+TGG | + | chr7.3:27226228-27226247 | None:intergenic | 60.0% | |
| CCACACCCGCTGCACCAGAA+CGG | - | chr7.3:27226347-27226366 | MS.gene51143:CDS | 65.0% | |
| TGCAGCGGGTGTGGATGTGC+TGG | + | chr7.3:27226341-27226360 | None:intergenic | 65.0% | |
| CCGTTCTGGTGCAGCGGGTG+TGG | + | chr7.3:27226350-27226369 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 27226184 | 27226813 | 27226184 | ID=MS.gene51143 |
| chr7.3 | mRNA | 27226184 | 27226813 | 27226184 | ID=MS.gene51143.t1;Parent=MS.gene51143 |
| chr7.3 | exon | 27226184 | 27226813 | 27226184 | ID=MS.gene51143.t1.exon1;Parent=MS.gene51143.t1 |
| chr7.3 | CDS | 27226184 | 27226813 | 27226184 | ID=cds.MS.gene51143.t1;Parent=MS.gene51143.t1 |
| Gene Sequence |
| Protein sequence |
