AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53524

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53524.t1 MTR_1g045130 95.192 208 7 1 1 208 1 205 1.24e-148 411
MS.gene53524.t1 MTR_2g018260 53.439 189 83 4 23 208 39 225 2.18e-66 204
MS.gene53524.t1 MTR_6g029230 34.507 142 92 1 34 175 48 188 4.15e-28 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53524.t1 AT5G35620 60.099 203 68 5 13 208 2 198 2.25e-81 241
MS.gene53524.t1 AT5G35620 60.099 203 68 5 13 208 2 198 2.25e-81 241
MS.gene53524.t1 AT5G35620 62.424 165 53 4 13 170 2 164 4.28e-66 201
MS.gene53524.t1 AT4G18040 52.273 176 81 2 34 207 60 234 9.92e-62 192
MS.gene53524.t1 AT1G29590 50.847 177 84 2 34 208 65 240 2.10e-61 191
MS.gene53524.t1 AT1G29550 50.847 177 84 2 34 208 65 240 3.18e-61 191
MS.gene53524.t1 AT5G18110 35.211 142 91 1 34 175 43 183 6.19e-28 105

Find 70 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTGACTTTCACTTGTTTA+AGG 0.094149 1.2:+27836488 MS.gene53524:CDS
CACTCGGGATCTTCCCATTT+TGG 0.167355 1.2:-27836522 None:intergenic
TAGAGAGAAGATGGAATTTC+TGG 0.177234 1.2:+27834802 MS.gene53524:CDS
AGGATGGGATTGAACCAAAA+TGG 0.219575 1.2:+27836508 MS.gene53524:CDS
ACCTAAACAAGGTGCTGCTT+GGG 0.298883 1.2:+27834842 MS.gene53524:CDS
TCGATACCGTTGAAGAGTTT+TGG 0.298995 1.2:+27834892 MS.gene53524:CDS
GCTTTGATTGGCGAGCAATT+TGG 0.311257 1.2:+27836726 MS.gene53524:CDS
AAGATTGCCCTTTGATTTGC+TGG 0.317297 1.2:-27836571 None:intergenic
GGAAGTGGCGGCGGCTGTTC+CGG 0.330565 1.2:+27834740 MS.gene53524:CDS
CCCCAAGCAGCACCTTGTTT+AGG 0.348201 1.2:-27834843 None:intergenic
AGCAGCACCTTGTTTAGGTT+TGG 0.348367 1.2:-27834838 None:intergenic
TCCGGTGCCGGTGCGGGTAC+CGG 0.370120 1.2:-27834759 None:intergenic
CTTTCCTCCATTGGCACACT+CGG 0.373760 1.2:-27836538 None:intergenic
AGCATTGCCAGGCAACTTGC+TGG 0.374886 1.2:-27836469 None:intergenic
CCCTGTCTCCGGTGCCGGTG+CGG 0.405136 1.2:-27834766 None:intergenic
CACCTTGTTTAGGTTTGGAT+TGG 0.410534 1.2:-27834833 None:intergenic
CCTGTCTCCGGTGCCGGTGC+GGG 0.416968 1.2:-27834765 None:intergenic
GACTTTCACTTGTTTAAGGA+TGG 0.426329 1.2:+27836492 MS.gene53524:CDS
TTTAGCCCTGTCTCCGGTGC+CGG 0.427478 1.2:-27834771 None:intergenic
CCCGCACCGGCACCGGAGAC+AGG 0.427726 1.2:+27834765 MS.gene53524:CDS
GTATCGAGCCTTTGCACCTC+GGG 0.428704 1.2:-27837993 None:intergenic
CTCTACCGCGGCAGAGGAAG+TGG 0.436984 1.2:+27834725 MS.gene53524:CDS
GCATTGCCAGGCAACTTGCT+GGG 0.440224 1.2:-27836468 None:intergenic
AACCTAAACAAGGTGCTGCT+TGG 0.442662 1.2:+27834841 MS.gene53524:CDS
GGATGGGATTGAACCAAAAT+GGG 0.454728 1.2:+27836509 MS.gene53524:CDS
GAATAGTTGATGGCTTTGAT+TGG 0.459935 1.2:+27836714 MS.gene53524:intron
CGCGGCAGAGGAAGTGGCGG+CGG 0.461792 1.2:+27834731 MS.gene53524:CDS
TTTATAATGCAGATGAGCAT+TGG 0.470732 1.2:+27837828 MS.gene53524:intron
GGCTGTTCCGGTACCCGCAC+CGG 0.473752 1.2:+27834752 MS.gene53524:CDS
AACACAAACTAGAGAGAAGA+TGG 0.478210 1.2:+27834793 MS.gene53524:CDS
TCCGGTACCCGCACCGGCAC+CGG 0.484224 1.2:+27834758 MS.gene53524:CDS
GCGGTAGAGCCTTCAACGAT+TGG 0.484430 1.2:-27834711 None:intergenic
GAGAGTCCACTTTCCTCCAT+TGG 0.488880 1.2:-27836547 None:intergenic
GACTCTCACCAGCAAATCAA+AGG 0.498552 1.2:+27836563 MS.gene53524:CDS
TTCAAGCCCAGCAAGTTGCC+TGG 0.503711 1.2:+27836462 MS.gene53524:CDS
TGCGAGTGTACGCCAGTGGC+AGG 0.504625 1.2:+27836776 MS.gene53524:CDS
TGCTAAAACCCGAGGTGCAA+AGG 0.508101 1.2:+27837985 MS.gene53524:CDS
ACTTTCACTTGTTTAAGGAT+GGG 0.509759 1.2:+27836493 MS.gene53524:CDS
TGAAGGCTCTACCGCGGCAG+AGG 0.510636 1.2:+27834719 MS.gene53524:CDS
AAGTGAAAGTCAGCATTGCC+AGG 0.511306 1.2:-27836480 None:intergenic
TTGGGGAACCACTCTTCGTA+AGG 0.512191 1.2:+27834860 MS.gene53524:CDS
GGAGACACGGAGGACATTTG+CGG 0.521047 1.2:+27836747 MS.gene53524:CDS
TGTATCGAGCCTTTGCACCT+CGG 0.539970 1.2:-27837994 None:intergenic
ACGAAAGTTTGTCCTGCCAC+TGG 0.555236 1.2:-27836788 None:intergenic
ACAACAGAACCAATCGTTGA+AGG 0.556771 1.2:+27834702 MS.gene53524:CDS
CCGCACCGGCACCGGAGACA+GGG 0.557169 1.2:+27834766 MS.gene53524:CDS
CGCCGCCACTTCCTCTGCCG+CGG 0.557840 1.2:-27834730 None:intergenic
TTTCCTCCATTGGCACACTC+GGG 0.558375 1.2:-27836537 None:intergenic
AGATGAGCATTGGAAGGAAG+TGG 0.568986 1.2:+27837838 MS.gene53524:intron
GAGCATTGGAAGGAAGTGGA+AGG 0.573080 1.2:+27837842 MS.gene53524:CDS
GAAGATCCCGAGTGTGCCAA+TGG 0.580686 1.2:+27836531 MS.gene53524:CDS
TAATGCAGATGAGCATTGGA+AGG 0.587446 1.2:+27837832 MS.gene53524:intron
GGAGTAAACCTTACGAAGAG+TGG 0.590798 1.2:-27834868 None:intergenic
AAACTCTTCAACGGTATCGA+AGG 0.596284 1.2:-27834889 None:intergenic
TGGCGAGCAATTTGGAGACA+CGG 0.605060 1.2:+27836734 MS.gene53524:CDS
ACTCTCACCAGCAAATCAAA+GGG 0.610543 1.2:+27836564 MS.gene53524:CDS
AGCAGCAAATGAGTCTAACC+AGG 0.617559 1.2:+27836824 MS.gene53524:CDS
CCTAAACAAGGTGCTGCTTG+GGG 0.622835 1.2:+27834843 MS.gene53524:CDS
TACCGCGGCAGAGGAAGTGG+CGG 0.635065 1.2:+27834728 MS.gene53524:CDS
GGCAGGACAAACTTTCGTTG+TGG 0.637833 1.2:+27836793 MS.gene53524:CDS
AGTGTGCCAATGGAGGAAAG+TGG 0.640587 1.2:+27836541 MS.gene53524:CDS
GATCCCGAGTGTGCCAATGG+AGG 0.645027 1.2:+27836534 MS.gene53524:CDS
TAGTTGCGAGTGTACGCCAG+TGG 0.651527 1.2:+27836772 MS.gene53524:CDS
GTACCACCAAAACTCTTCAA+CGG 0.656585 1.2:-27834898 None:intergenic
AATTACAGTTTCGAGCCACA+TGG 0.668557 1.2:-27836598 None:intergenic
CAGGAAGATGCTAAAACCCG+AGG 0.674698 1.2:+27837977 MS.gene53524:intron
AACCAATCCAAACCTAAACA+AGG 0.681137 1.2:+27834831 MS.gene53524:CDS
AGGGCAATCTTGATACCATG+TGG 0.704318 1.2:+27836583 MS.gene53524:CDS
AATCGTTGAAGGCTCTACCG+CGG 0.717531 1.2:+27834713 MS.gene53524:CDS
CGAGCAATTTGGAGACACGG+AGG 0.752078 1.2:+27836737 MS.gene53524:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATAGAAAATAAAAAAAA+CGG - chr1.2:27834942-27834961 None:intergenic 10.0%
!!! TATTGAAAATTTATTTTGAA+TGG + chr1.2:27835185-27835204 MS.gene53524:intron 10.0%
!!! TTTATTTTCTATTGAATTTA+AGG + chr1.2:27834947-27834966 MS.gene53524:intron 10.0%
!!! TTTTTTTTAAAGAATATATG+GGG + chr1.2:27836645-27836664 MS.gene53524:intron 10.0%
!!! TTATTCAATAAGCTATTTTA+GGG + chr1.2:27837090-27837109 MS.gene53524:intron 15.0%
!!! TTTATTCAATAAGCTATTTT+AGG + chr1.2:27837089-27837108 MS.gene53524:intron 15.0%
!!! TTTTTGATGATTTTATTTGT+GGG + chr1.2:27835114-27835133 MS.gene53524:intron 15.0%
!!! TTTTTTGATGATTTTATTTG+TGG + chr1.2:27835113-27835132 MS.gene53524:intron 15.0%
!! TAATTAGAATCTAAGAGATA+TGG + chr1.2:27835238-27835257 MS.gene53524:intron 20.0%
!! TGATATATGATAGAACATTA+TGG + chr1.2:27835619-27835638 MS.gene53524:intron 20.0%
!! TTAAAAAAGTGATGTAAATG+CGG + chr1.2:27835866-27835885 MS.gene53524:intron 20.0%
!!! AAGTTGTTTTTTAGTAGATA+TGG + chr1.2:27835280-27835299 MS.gene53524:intron 20.0%
!!! TATTGATTTTTGTAGATCTT+AGG + chr1.2:27835019-27835038 MS.gene53524:intron 20.0%
!!! TGTTTTTATTGAATAGTTGA+TGG + chr1.2:27836704-27836723 MS.gene53524:intron 20.0%
!!! TTTTGATGATTTTATTTGTG+GGG + chr1.2:27835115-27835134 MS.gene53524:intron 20.0%
! AAAAAAAAAAACCGAAGGAA+CGG - chr1.2:27837433-27837452 None:intergenic 25.0%
! AGAAGATTATAACCCTTTAA+AGG - chr1.2:27836226-27836245 None:intergenic 25.0%
! ATGATTGTTGTCACTAATTA+TGG + chr1.2:27836681-27836700 MS.gene53524:intron 25.0%
! TAAACATCGATAATTAGTTG+TGG + chr1.2:27837622-27837641 MS.gene53524:intron 25.0%
! TATATGATAGAACATTATGG+CGG + chr1.2:27835622-27835641 MS.gene53524:intron 25.0%
! TCAACATTCAAATCAACATA+AGG - chr1.2:27837596-27837615 None:intergenic 25.0%
! TTATTATTATTTGAACGTCC+AGG + chr1.2:27836369-27836388 MS.gene53524:intron 25.0%
! TTGTTGTCATTCAGAATTTA+AGG - chr1.2:27837468-27837487 None:intergenic 25.0%
!! AGAAAAGCATATAAATTTGC+AGG - chr1.2:27836875-27836894 None:intergenic 25.0%
!!! ATGTTGGTATTTTTAGACTT+AGG + chr1.2:27837032-27837051 MS.gene53524:intron 25.0%
!!! GATTTTTTTGTTGACTTCTT+TGG + chr1.2:27837339-27837358 MS.gene53524:intron 25.0%
!!! TATTTTAGGGAGATTAATTG+AGG + chr1.2:27837103-27837122 MS.gene53524:intron 25.0%
!!! TCCTTCGGTTTTTTTTTTTT+GGG + chr1.2:27837434-27837453 MS.gene53524:intron 25.0%
!!! TGTTGGTATTTTTAGACTTA+GGG + chr1.2:27837033-27837052 MS.gene53524:intron 25.0%
!!! TTCCTTCGGTTTTTTTTTTT+TGG + chr1.2:27837433-27837452 MS.gene53524:intron 25.0%
!!! TTGAATGGTTTTGTTATGTT+TGG + chr1.2:27835200-27835219 MS.gene53524:intron 25.0%
!!! TTTGATGATTTTATTTGTGG+GGG + chr1.2:27835116-27835135 MS.gene53524:intron 25.0%
!!! TTTTAGTTCCAGAATTATGA+TGG + chr1.2:27837315-27837334 MS.gene53524:intron 25.0%
AAAAATCGCCATCATAATTC+TGG - chr1.2:27837326-27837345 None:intergenic 30.0%
AAAGAATATATGGGGTAATC+TGG + chr1.2:27836653-27836672 MS.gene53524:intron 30.0%
ACTTTCACTTGTTTAAGGAT+GGG + chr1.2:27836493-27836512 MS.gene53524:CDS 30.0%
ATATTGTTGTACCTAGACAA+TGG + chr1.2:27836933-27836952 MS.gene53524:intron 30.0%
ATTCTGAATGACAACAAAAC+AGG + chr1.2:27837471-27837490 MS.gene53524:intron 30.0%
CACGTATTGTAGAAAAAAGA+TGG + chr1.2:27835432-27835451 MS.gene53524:intron 30.0%
CATCATTATTTACCAAAACC+TGG - chr1.2:27836390-27836409 None:intergenic 30.0%
CTTACATGTTCAATTGTTAC+AGG + chr1.2:27837958-27837977 MS.gene53524:intron 30.0%
GCAGTAACCACTTAAAATTT+AGG - chr1.2:27837922-27837941 None:intergenic 30.0%
GTCATTTCAACATTAAAGTC+TGG + chr1.2:27837361-27837380 MS.gene53524:intron 30.0%
TAGTCAAATGTGCCTTTAAA+GGG + chr1.2:27836211-27836230 MS.gene53524:intron 30.0%
TATAAGAATAACAACAGACC+TGG - chr1.2:27836845-27836864 None:intergenic 30.0%
TCACATATATACTGAACACT+GGG + chr1.2:27836263-27836282 MS.gene53524:intron 30.0%
TCATTTCTTCTTTCCCAAAA+GGG + chr1.2:27837508-27837527 MS.gene53524:intron 30.0%
TGTAAGCACAAAACAACTTA+TGG + chr1.2:27837159-27837178 MS.gene53524:intron 30.0%
TTATTGCGTTGAATACTAGA+CGG + chr1.2:27835358-27835377 MS.gene53524:intron 30.0%
TTCACATATATACTGAACAC+TGG + chr1.2:27836262-27836281 MS.gene53524:intron 30.0%
TTTATAATGCAGATGAGCAT+TGG + chr1.2:27837828-27837847 MS.gene53524:intron 30.0%
! GATTTTTGTAGATCTTAGGA+GGG + chr1.2:27835023-27835042 MS.gene53524:intron 30.0%
! GTTTCTCTTATAGTTTTCCT+AGG - chr1.2:27835568-27835587 None:intergenic 30.0%
! TGATTTTTGTAGATCTTAGG+AGG + chr1.2:27835022-27835041 MS.gene53524:intron 30.0%
! TTCTGCTCCTAAATTTTAAG+TGG + chr1.2:27837912-27837931 MS.gene53524:intron 30.0%
!! CAGGTTTTGGTAAATAATGA+TGG + chr1.2:27836388-27836407 MS.gene53524:intron 30.0%
!!! CCTTCGGTTTTTTTTTTTTG+GGG + chr1.2:27837435-27837454 MS.gene53524:intron 30.0%
!!! TTGGTGGTACGTTTTTTTTA+GGG + chr1.2:27834911-27834930 MS.gene53524:intron 30.0%
!!! TTTGGTGGTACGTTTTTTTT+AGG + chr1.2:27834910-27834929 MS.gene53524:intron 30.0%
AACACAAACTAGAGAGAAGA+TGG + chr1.2:27834793-27834812 MS.gene53524:CDS 35.0%
AACCAATCCAAACCTAAACA+AGG + chr1.2:27834831-27834850 MS.gene53524:CDS 35.0%
AATGCCTTCTTAAAGCATGT+CGG + chr1.2:27837266-27837285 MS.gene53524:intron 35.0%
CCCCAAAAAAAAAAAACCGA+AGG - chr1.2:27837438-27837457 None:intergenic 35.0%
CGGTATATGACACAACATTA+TGG + chr1.2:27835886-27835905 MS.gene53524:intron 35.0%
GACTTTCACTTGTTTAAGGA+TGG + chr1.2:27836492-27836511 MS.gene53524:CDS 35.0%
GTAGTCAAATGTGCCTTTAA+AGG + chr1.2:27836210-27836229 MS.gene53524:intron 35.0%
GTCATTTCTTCTTTCCCAAA+AGG + chr1.2:27837507-27837526 MS.gene53524:intron 35.0%
TAGAGAGAAGATGGAATTTC+TGG + chr1.2:27834802-27834821 MS.gene53524:CDS 35.0%
TATTGCGTTGAATACTAGAC+GGG + chr1.2:27835359-27835378 MS.gene53524:intron 35.0%
TTCCTTAAACATCAACGTTG+CGG + chr1.2:27837710-27837729 MS.gene53524:intron 35.0%
TTTGGGAAAGAAGAAATGAC+AGG - chr1.2:27837507-27837526 None:intergenic 35.0%
! AATGCTTGGTTGTTGTTGTT+TGG + chr1.2:27835667-27835686 MS.gene53524:intron 35.0%
! GACGTTTAGATTTTGCAGTA+AGG + chr1.2:27835494-27835513 MS.gene53524:intron 35.0%
! TTATTTGAACGTCCAGGTTT+TGG + chr1.2:27836375-27836394 MS.gene53524:intron 35.0%
!! GAATAGTTGATGGCTTTGAT+TGG + chr1.2:27836714-27836733 MS.gene53524:intron 35.0%
!! TGCTGACTTTCACTTGTTTA+AGG + chr1.2:27836488-27836507 MS.gene53524:CDS 35.0%
!! TTGACACGAGCTTTTTGTTT+TGG + chr1.2:27837803-27837822 MS.gene53524:intron 35.0%
AAACCAGACTTAGGTGGTTT+GGG + chr1.2:27835458-27835477 MS.gene53524:intron 40.0%
AACGGCAATAAGTTCAGAAG+AGG - chr1.2:27837415-27837434 None:intergenic 40.0%
AATAAGTTCAGAAGAGGGGA+GGG - chr1.2:27837409-27837428 None:intergenic 40.0%
AATTACAGTTTCGAGCCACA+TGG - chr1.2:27836601-27836620 None:intergenic 40.0%
ACAACAGAACCAATCGTTGA+AGG + chr1.2:27834702-27834721 MS.gene53524:CDS 40.0%
ACACTTCCTTCACATTGTGA+AGG - chr1.2:27837003-27837022 None:intergenic 40.0%
ACGGCAATAAGTTCAGAAGA+GGG - chr1.2:27837414-27837433 None:intergenic 40.0%
ACTCTCACCAGCAAATCAAA+GGG + chr1.2:27836564-27836583 MS.gene53524:CDS 40.0%
AGAGTAGATCAGATGAAGAG+TGG + chr1.2:27835517-27835536 MS.gene53524:intron 40.0%
AGAGTGGTCAAATAGCTAGA+CGG + chr1.2:27835533-27835552 MS.gene53524:intron 40.0%
AGGATGGGATTGAACCAAAA+TGG + chr1.2:27836508-27836527 MS.gene53524:CDS 40.0%
ATCTTAGGAGGGAGATACTT+AGG + chr1.2:27835034-27835053 MS.gene53524:intron 40.0%
CAATATAGTCCATCGAGTAG+CGG - chr1.2:27837744-27837763 None:intergenic 40.0%
GAAAGAAGAAATGACAGGAC+AGG - chr1.2:27837502-27837521 None:intergenic 40.0%
GGATGGGATTGAACCAAAAT+GGG + chr1.2:27836509-27836528 MS.gene53524:CDS 40.0%
GTACCACCAAAACTCTTCAA+CGG - chr1.2:27834901-27834920 None:intergenic 40.0%
TACCGCAACGTTGATGTTTA+AGG - chr1.2:27837715-27837734 None:intergenic 40.0%
TCTTAGGAGGGAGATACTTA+GGG + chr1.2:27835035-27835054 MS.gene53524:intron 40.0%
TGAACTTATTGCCGTTCCTT+CGG + chr1.2:27837419-27837438 MS.gene53524:intron 40.0%
! AAACTCTTCAACGGTATCGA+AGG - chr1.2:27834892-27834911 None:intergenic 40.0%
! CACCTTGTTTAGGTTTGGAT+TGG - chr1.2:27834836-27834855 None:intergenic 40.0%
! GTCATCTCCTATGAGTTTTG+AGG - chr1.2:27837781-27837800 None:intergenic 40.0%
! TCACAATGTGAAGGAAGTGT+CGG + chr1.2:27837003-27837022 MS.gene53524:intron 40.0%
! TCGATACCGTTGAAGAGTTT+TGG + chr1.2:27834892-27834911 MS.gene53524:CDS 40.0%
! TGGTTGTTGTTGTCGTTGTT+TGG + chr1.2:27835960-27835979 MS.gene53524:intron 40.0%
!! AAGATTGCCCTTTGATTTGC+TGG - chr1.2:27836574-27836593 None:intergenic 40.0%
!! ATACCGTTGAAGAGTTTTGG+TGG + chr1.2:27834895-27834914 MS.gene53524:CDS 40.0%
!! GTTACCGACATGCTTTAAGA+AGG - chr1.2:27837273-27837292 None:intergenic 40.0%
!! TAATGCAGATGAGCATTGGA+AGG + chr1.2:27837832-27837851 MS.gene53524:intron 40.0%
!!! ATCTCCTATGAGTTTTGAGG+TGG - chr1.2:27837778-27837797 None:intergenic 40.0%
AAGTGAAAGTCAGCATTGCC+AGG - chr1.2:27836483-27836502 None:intergenic 45.0%
AGAAGAAATGACAGGACAGG+AGG - chr1.2:27837499-27837518 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAGTT+GGG + chr1.2:27835405-27835424 MS.gene53524:intron 45.0%
AGATGGTCGAAACCAGACTT+AGG + chr1.2:27835449-27835468 MS.gene53524:intron 45.0%
AGCAGCAAATGAGTCTAACC+AGG + chr1.2:27836824-27836843 MS.gene53524:CDS 45.0%
AGGGCAATCTTGATACCATG+TGG + chr1.2:27836583-27836602 MS.gene53524:CDS 45.0%
ATAAGTTCAGAAGAGGGGAG+GGG - chr1.2:27837408-27837427 None:intergenic 45.0%
CAATAAGTTCAGAAGAGGGG+AGG - chr1.2:27837410-27837429 None:intergenic 45.0%
CCAAGCATTATCCCACTACA+TGG - chr1.2:27835656-27835675 None:intergenic 45.0%
CGGCAATAAGTTCAGAAGAG+GGG - chr1.2:27837413-27837432 None:intergenic 45.0%
GAAACCAGACTTAGGTGGTT+TGG + chr1.2:27835457-27835476 MS.gene53524:intron 45.0%
GAAGAAATGACAGGACAGGA+GGG - chr1.2:27837498-27837517 None:intergenic 45.0%
GACTCTCACCAGCAAATCAA+AGG + chr1.2:27836563-27836582 MS.gene53524:CDS 45.0%
GCTTTGATTGGCGAGCAATT+TGG + chr1.2:27836726-27836745 MS.gene53524:CDS 45.0%
GGAGTAAACCTTACGAAGAG+TGG - chr1.2:27834871-27834890 None:intergenic 45.0%
TCTCACGCATACCATTGTCT+AGG - chr1.2:27836947-27836966 None:intergenic 45.0%
TTCACGCCTTCACAATGTGA+AGG + chr1.2:27836994-27837013 MS.gene53524:intron 45.0%
! AAAGTCTGGTTTATGTCCCC+AGG + chr1.2:27837375-27837394 MS.gene53524:intron 45.0%
! AGCAGCACCTTGTTTAGGTT+TGG - chr1.2:27834841-27834860 None:intergenic 45.0%
! AGCGAAGATGAAGCCCTTTT+GGG - chr1.2:27837524-27837543 None:intergenic 45.0%
! CCATGTAGTGGGATAATGCT+TGG + chr1.2:27835653-27835672 MS.gene53524:intron 45.0%
! TTCACATTGTGAAGGCGTGA+AGG - chr1.2:27836995-27837014 None:intergenic 45.0%
!! AACCTAAACAAGGTGCTGCT+TGG + chr1.2:27834841-27834860 MS.gene53524:CDS 45.0%
!! ACCTAAACAAGGTGCTGCTT+GGG + chr1.2:27834842-27834861 MS.gene53524:CDS 45.0%
!! AGATGAGCATTGGAAGGAAG+TGG + chr1.2:27837838-27837857 MS.gene53524:intron 45.0%
!!! TCCTATGAGTTTTGAGGTGG+CGG - chr1.2:27837775-27837794 None:intergenic 45.0%
!!! TTGTGTTTTAGCCCTGTCTC+CGG - chr1.2:27834780-27834799 None:intergenic 45.0%
!!! ATTCTTTAAAAAAAAAAAAA+AGG - chr1.2:27836640-27836659 None:intergenic 5.0%
!!! TTTTTTTTTAAAGAATATAT+GGG + chr1.2:27836644-27836663 MS.gene53524:intron 5.0%
!!! TTTTTTTTTTAAAGAATATA+TGG + chr1.2:27836643-27836662 MS.gene53524:intron 5.0%
AATCGTTGAAGGCTCTACCG+CGG + chr1.2:27834713-27834732 MS.gene53524:CDS 50.0%
ACGAAAGTTTGTCCTGCCAC+TGG - chr1.2:27836791-27836810 None:intergenic 50.0%
AGTGTGCCAATGGAGGAAAG+TGG + chr1.2:27836541-27836560 MS.gene53524:CDS 50.0%
ATGCCCAAACCACCTAAGTC+TGG - chr1.2:27835464-27835483 None:intergenic 50.0%
CAGAGAGAGAGAGAGAGAGT+TGG + chr1.2:27835404-27835423 MS.gene53524:intron 50.0%
CAGGAAGATGCTAAAACCCG+AGG + chr1.2:27837977-27837996 MS.gene53524:intron 50.0%
CATCTTGTGCGGCTTTCTGA+TGG + chr1.2:27837547-27837566 MS.gene53524:intron 50.0%
CGGCGTTTGATCCATGTAGT+GGG + chr1.2:27835642-27835661 MS.gene53524:intron 50.0%
CTTTCCTCCATTGGCACACT+CGG - chr1.2:27836541-27836560 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGTTG+GGG + chr1.2:27835406-27835425 MS.gene53524:intron 50.0%
GAGAGTCCACTTTCCTCCAT+TGG - chr1.2:27836550-27836569 None:intergenic 50.0%
GGCAGGACAAACTTTCGTTG+TGG + chr1.2:27836793-27836812 MS.gene53524:CDS 50.0%
GGTCAAATAGCTAGACGGAG+AGG + chr1.2:27835538-27835557 MS.gene53524:intron 50.0%
TAAGTTCAGAAGAGGGGAGG+GGG - chr1.2:27837407-27837426 None:intergenic 50.0%
TCAGAAAGCCGCACAAGATG+AGG - chr1.2:27837547-27837566 None:intergenic 50.0%
TCTTCGCTCCTCATCTTGTG+CGG + chr1.2:27837536-27837555 MS.gene53524:intron 50.0%
TGCTAAAACCCGAGGTGCAA+AGG + chr1.2:27837985-27838004 MS.gene53524:CDS 50.0%
TGGCGAGCAATTTGGAGACA+CGG + chr1.2:27836734-27836753 MS.gene53524:CDS 50.0%
TGGTCGAAACCAGACTTAGG+TGG + chr1.2:27835452-27835471 MS.gene53524:intron 50.0%
TGTATCGAGCCTTTGCACCT+CGG - chr1.2:27837997-27838016 None:intergenic 50.0%
TTGGGGAACCACTCTTCGTA+AGG + chr1.2:27834860-27834879 MS.gene53524:CDS 50.0%
TTTCCTCCATTGGCACACTC+GGG - chr1.2:27836540-27836559 None:intergenic 50.0%
! CACTCGGGATCTTCCCATTT+TGG - chr1.2:27836525-27836544 None:intergenic 50.0%
! CTTAGGTGGTTTGGGCATGT+AGG + chr1.2:27835466-27835485 MS.gene53524:intron 50.0%
! GAGCGAAGATGAAGCCCTTT+TGG - chr1.2:27837525-27837544 None:intergenic 50.0%
!! CAATGTGAAGGAAGTGTCGG+TGG + chr1.2:27837006-27837025 MS.gene53524:intron 50.0%
!! CCTAAACAAGGTGCTGCTTG+GGG + chr1.2:27834843-27834862 MS.gene53524:CDS 50.0%
!! GAAGTGTCGGTGGTGAATGT+TGG + chr1.2:27837016-27837035 MS.gene53524:intron 50.0%
!! GAGCATTGGAAGGAAGTGGA+AGG + chr1.2:27837842-27837861 MS.gene53524:CDS 50.0%
!!! CCTATGAGTTTTGAGGTGGC+GGG - chr1.2:27837774-27837793 None:intergenic 50.0%
AAGTAGCTGACCCCACCTAG+TGG + chr1.2:27835922-27835941 MS.gene53524:intron 55.0%
ACTAGGTGGGGTCAGCTACT+TGG - chr1.2:27835923-27835942 None:intergenic 55.0%
AGCATTGCCAGGCAACTTGC+TGG - chr1.2:27836472-27836491 None:intergenic 55.0%
AGTAGCTGACCCCACCTAGT+GGG + chr1.2:27835923-27835942 MS.gene53524:intron 55.0%
CCCGCCACCTCAAAACTCAT+AGG + chr1.2:27837771-27837790 MS.gene53524:intron 55.0%
CGAGCAATTTGGAGACACGG+AGG + chr1.2:27836737-27836756 MS.gene53524:CDS 55.0%
CTAGACGGAGAGGAAGACCT+AGG + chr1.2:27835548-27835567 MS.gene53524:intron 55.0%
GAAGATCCCGAGTGTGCCAA+TGG + chr1.2:27836531-27836550 MS.gene53524:CDS 55.0%
GCGGCGTTTGATCCATGTAG+TGG + chr1.2:27835641-27835660 MS.gene53524:intron 55.0%
GCGGTAGAGCCTTCAACGAT+TGG - chr1.2:27834714-27834733 None:intergenic 55.0%
GGAGACACGGAGGACATTTG+CGG + chr1.2:27836747-27836766 MS.gene53524:CDS 55.0%
GTAGCTATGCCGCTACTCGA+TGG + chr1.2:27837732-27837751 MS.gene53524:intron 55.0%
GTATCGAGCCTTTGCACCTC+GGG - chr1.2:27837996-27838015 None:intergenic 55.0%
TAGTTGCGAGTGTACGCCAG+TGG + chr1.2:27836772-27836791 MS.gene53524:CDS 55.0%
TGACCCCACCTAGTGGGATA+AGG + chr1.2:27835929-27835948 MS.gene53524:intron 55.0%
TTCAAGCCCAGCAAGTTGCC+TGG + chr1.2:27836462-27836481 MS.gene53524:CDS 55.0%
! CCCCAAGCAGCACCTTGTTT+AGG - chr1.2:27834846-27834865 None:intergenic 55.0%
! GCATTGCCAGGCAACTTGCT+GGG - chr1.2:27836471-27836490 None:intergenic 55.0%
! GGTGGTTTGGGCATGTAGGT+AGG + chr1.2:27835470-27835489 MS.gene53524:intron 55.0%
AGCCGCCTTATCCCACTAGG+TGG - chr1.2:27835937-27835956 None:intergenic 60.0%
CCAAGCCGCCTTATCCCACT+AGG - chr1.2:27835940-27835959 None:intergenic 60.0%
CCCCACCTAGTGGGATAAGG+CGG + chr1.2:27835932-27835951 MS.gene53524:intron 60.0%
CCGCCTTATCCCACTAGGTG+GGG - chr1.2:27835935-27835954 None:intergenic 60.0%
CCTAGTGGGATAAGGCGGCT+TGG + chr1.2:27835937-27835956 MS.gene53524:intron 60.0%
GATCCCGAGTGTGCCAATGG+AGG + chr1.2:27836534-27836553 MS.gene53524:CDS 60.0%
GCCGCCTTATCCCACTAGGT+GGG - chr1.2:27835936-27835955 None:intergenic 60.0%
TTTAGCCCTGTCTCCGGTGC+CGG - chr1.2:27834774-27834793 None:intergenic 60.0%
! AGTGGGATAAGGCGGCTTGG+TGG + chr1.2:27835940-27835959 MS.gene53524:intron 60.0%
CTCTACCGCGGCAGAGGAAG+TGG + chr1.2:27834725-27834744 MS.gene53524:CDS 65.0%
TACCGCGGCAGAGGAAGTGG+CGG + chr1.2:27834728-27834747 MS.gene53524:CDS 65.0%
TGAAGGCTCTACCGCGGCAG+AGG + chr1.2:27834719-27834738 MS.gene53524:CDS 65.0%
TGCGAGTGTACGCCAGTGGC+AGG + chr1.2:27836776-27836795 MS.gene53524:CDS 65.0%
GGAAGTGGCGGCGGCTGTTC+CGG + chr1.2:27834740-27834759 MS.gene53524:CDS 70.0%
GGCTGTTCCGGTACCCGCAC+CGG + chr1.2:27834752-27834771 MS.gene53524:CDS 70.0%
! AGGGGAGGGGGCGTAAGCCT+GGG - chr1.2:27837395-27837414 None:intergenic 70.0%
CGCCGCCACTTCCTCTGCCG+CGG - chr1.2:27834733-27834752 None:intergenic 75.0%
CGCGGCAGAGGAAGTGGCGG+CGG + chr1.2:27834731-27834750 MS.gene53524:CDS 75.0%
! CCGCACCGGCACCGGAGACA+GGG + chr1.2:27834766-27834785 MS.gene53524:CDS 75.0%
! GAGGGGAGGGGGCGTAAGCC+TGG - chr1.2:27837396-27837415 None:intergenic 75.0%
! GGGGAGGGGGCGTAAGCCTG+GGG - chr1.2:27837394-27837413 None:intergenic 75.0%
! TCCGGTACCCGCACCGGCAC+CGG + chr1.2:27834758-27834777 MS.gene53524:CDS 75.0%
!! CCCTGTCTCCGGTGCCGGTG+CGG - chr1.2:27834769-27834788 None:intergenic 75.0%
!! CCTGTCTCCGGTGCCGGTGC+GGG - chr1.2:27834768-27834787 None:intergenic 75.0%
!! TCCGGTGCCGGTGCGGGTAC+CGG - chr1.2:27834762-27834781 None:intergenic 75.0%
!! CCCGCACCGGCACCGGAGAC+AGG + chr1.2:27834765-27834784 MS.gene53524:CDS 80.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 27834696 27838024 27834696 ID=MS.gene53524
chr1.2 mRNA 27834696 27838024 27834696 ID=MS.gene53524.t1;Parent=MS.gene53524
chr1.2 exon 27834696 27834916 27834696 ID=MS.gene53524.t1.exon1;Parent=MS.gene53524.t1
chr1.2 CDS 27834696 27834916 27834696 ID=cds.MS.gene53524.t1;Parent=MS.gene53524.t1
chr1.2 exon 27836446 27836614 27836446 ID=MS.gene53524.t1.exon2;Parent=MS.gene53524.t1
chr1.2 CDS 27836446 27836614 27836446 ID=cds.MS.gene53524.t1;Parent=MS.gene53524.t1
chr1.2 exon 27836720 27836845 27836720 ID=MS.gene53524.t1.exon3;Parent=MS.gene53524.t1
chr1.2 CDS 27836720 27836845 27836720 ID=cds.MS.gene53524.t1;Parent=MS.gene53524.t1
chr1.2 exon 27837840 27837905 27837840 ID=MS.gene53524.t1.exon4;Parent=MS.gene53524.t1
chr1.2 CDS 27837840 27837905 27837840 ID=cds.MS.gene53524.t1;Parent=MS.gene53524.t1
chr1.2 exon 27837980 27838024 27837980 ID=MS.gene53524.t1.exon5;Parent=MS.gene53524.t1
chr1.2 CDS 27837980 27838024 27837980 ID=cds.MS.gene53524.t1;Parent=MS.gene53524.t1
Gene Sequence

>MS.gene53524

ATGGCAACAACAGAACCAATCGTTGAAGGCTCTACCGCGGCAGAGGAAGTGGCGGCGGCTGTTCCGGTACCCGCACCGGCACCGGAGACAGGGCTAAAACACAAACTAGAGAGAAGATGGAATTTCTGGTTCGATAACCAATCCAAACCTAAACAAGGTGCTGCTTGGGGAACCACTCTTCGTAAGGTTTACTCCTTCGATACCGTTGAAGAGTTTTGGTGGTACGTTTTTTTTAGGGTTCTTCCGTTTTTTTTATTTTCTATTGAATTTAAGGTTGCAATCTGAAATATTATGTTCAATTTGATTGAATTTATGTAAATGTTTATTGATTTTTGTAGATCTTAGGAGGGAGATACTTAGGGCATGTTTGTAACAAAGAATATTTGATAGAAATAAAATTTTGTAACTGAAAAATTATTTTTTGATGATTTTATTTGTGGGGGTATGTTTGTTTTTGTAATTGATTATTTGTTAGAAATTCACTTTTTCTATTGAAAATTTATTTTGAATGGTTTTGTTATGTTTGGTTAAGCTTTATAGATTAATTAGAATCTAAGAGATATGGATTTTTTTATTAGAATGAGAAGTTGTTTTTTAGTAGATATGGATTTTCTTGATTAGATTGAGAAGTTGTTTTCTAGTAGCTTGTAAAAGAGATAAGATTATTGCGTTGAATACTAGACGGGATAAGCTTAGAAATTACAATATCAGAGAGAGAGAGAGAGAGTTGGGGTAGCACGTATTGTAGAAAAAAGATGGTCGAAACCAGACTTAGGTGGTTTGGGCATGTAGGTAGGAGACGTTTAGATTTTGCAGTAAGGAGAGTAGATCAGATGAAGAGTGGTCAAATAGCTAGACGGAGAGGAAGACCTAGGAAAACTATAAGAGAAACTATTATAAGAAAGATGTAGAGTTATGATATATGATATATGATAGAACATTATGGCGGCGTTTGATCCATGTAGTGGGATAATGCTTGGTTGTTGTTGTTTGGTTTTGATTGTGTTTGTTTCAGCTTTTTAAGTTAATCAGAATCTAAAAGATTTGATTTTTTTTTTTTTTTGATTAATTTGAGAAGTTGTTTTTAGTAGTTTCTATAAGAATATGATTTTTGTGTCAAAAAAAAATTAGAATATGATTTTAATGAGTTAATTTATTATGATGAGAAGTTTAAAAAAGTGATGTAAATGCGGTATATGACACAACATTATGGCATCGTTTGATCCAAGTAGCTGACCCCACCTAGTGGGATAAGGCGGCTTGGTGGTTGTTGTTGTCGTTGTTTGGTTTTGATTGTGTTTGTTTAAACTTTTGAAATTAATCAGAATCTAAACGATTTGATTTTTTGTTTAATTTGAGCAGTTGTTTTTAGTAGATTATAAAAGTGTCTGATTTTAATGAGTGAGAAGTTTAAACAATTGATGTTTACATTCACTATTATTATCAATAAACACAAATCAGCTTATAATTCAAATAAAAGTATCTATTTTTTTTATTTGATAATAATTTTTAGTGTAGTCAAATGTGCCTTTAAAGGGTTATAATCTTCTGCTTGTCAGTAAAAATTTCACATATATACTGAACACTGGGTCTTTTTATTTCATTTTATTCATTGCTGCTATATTATATTATTATTTTCATTGCTGCTATATTATATTATTATAGTAGTATTCTTATTATTATTTGAACGTCCAGGTTTTGGTAAATAATGATGGAAATTTAACGAAATTGTGATGTGTAAATTTTGTAGTTTGCATGATCAGATATTCAAGCCCAGCAAGTTGCCTGGCAATGCTGACTTTCACTTGTTTAAGGATGGGATTGAACCAAAATGGGAAGATCCCGAGTGTGCCAATGGAGGAAAGTGGACTCTCACCAGCAAATCAAAGGGCAATCTTGATACCATGTGGCTCGAAACTGTAATTTGTTTTTATCACTAAACCTTTTTTTTTTTTTTAAAGAATATATGGGGTAATCTGGTTTTTATGATTGTTGTCACTAATTATGGTGTTTTTATTGAATAGTTGATGGCTTTGATTGGCGAGCAATTTGGAGACACGGAGGACATTTGCGGCGTAGTTGCGAGTGTACGCCAGTGGCAGGACAAACTTTCGTTGTGGACGAAAACAGCAGCAAATGAGTCTAACCAGGTCTGTTGTTATTCTTATAAATAATGCCTGCAAATTTATATGCTTTTCTAATTTATGTTATTTTGTCTGTATATGATTTTCTATTTTATATTGTTGTACCTAGACAATGGTATGCGTGAGAAAACTGTCTTACTTCAGCTAGAAAGCCTTCACGCCTTCACAATGTGAAGGAAGTGTCGGTGGTGAATGTTGGTATTTTTAGACTTAGGGTGTTTCTGAGTTAAGTAAGAGTTTAATTAATCATTTATTCAATAAGCTATTTTAGGGAGATTAATTGAGGTTGATACTTACTTTTCTGTTAAATTTATTGAAATGTAAGCACAAAACAACTTATGGACATTTCATTACGTGTAAGTTATTATTAAACACTAAAGTAAGTGTTTATATCATAAATAAAATAATCACAAGTAAGCTCTTTGAAATGCCTTCTTAAAGCATGTCGGTAACTCTTGAATGTGTATCATATCATTTTTAGTTCCAGAATTATGATGGCGATTTTTTTGTTGACTTCTTTGGTCATTTCAACATTAAAGTCTGGTTTATGTCCCCAGGCTTACGCCCCCTCCCCTCTTCTGAACTTATTGCCGTTCCTTCGGTTTTTTTTTTTTGGGGTTTTCAACCTTAAATTCTGAATGACAACAAAACAGGACCCTCCTGTCCTGTCATTTCTTCTTTCCCAAAAGGGCTTCATCTTCGCTCCTCATCTTGTGCGGCTTTCTGATGGCTTTTTCTGCAACTTTCTAGCAGCCTTATGTTGATTTGAATGTTGATACTAATAAACATCGATAATTAGTTGTGGTTTTGTTCTTTAATGTTGTGATTCTATTTTTTTAGCTCATGTATAGTATAAATTTCTTGTATTGTTTCCTTAAACATCAACGTTGCGGTAGCTATGCCGCTACTCGATGGACTATATTGATGTATTCCCGCCACCTCAAAACTCATAGGAGATGACATTTGACACGAGCTTTTTGTTTTGGAATTTATAATGCAGATGAGCATTGGAAGGAAGTGGAAGGAAATCATTGATGTTAGCGACAAGATGACATATAACTTTCATGTAAACTTCTGCTCCTAAATTTTAAGTGGTTACTGCATAAATATCTACTATTCTTACATGTTCAATTGTTACAGGAAGATGCTAAAACCCGAGGTGCAAAGGCTCGATACACTGTATAA
Protein sequence

>MS.gene53524.t1

MATTEPIVEGSTAAEEVAAAVPVPAPAPETGLKHKLERRWNFWFDNQSKPKQGAAWGTTLRKVYSFDTVEEFWCLHDQIFKPSKLPGNADFHLFKDGIEPKWEDPECANGGKWTLTSKSKGNLDTMWLETLMALIGEQFGDTEDICGVVASVRQWQDKLSLWTKTAANESNQMSIGRKWKEIIDVSDKMTYNFHEDAKTRGAKARYTV