AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53831


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53831.t1 MTR_7g015550 70.181 332 95 3 2 332 188 516 1.37e-159 469
MS.gene53831.t1 MTR_7g015390 70.783 332 95 1 1 332 187 516 1.84e-159 468
MS.gene53831.t1 MTR_7g015670 71.726 336 89 4 1 332 186 519 2.42e-159 468
MS.gene53831.t1 MTR_7g015510 70.482 332 94 3 2 332 188 516 4.34e-159 467
MS.gene53831.t1 MTR_4g052290 68.405 326 98 3 8 332 190 511 4.30e-149 441
MS.gene53831.t1 MTR_7g015320 66.189 349 96 5 1 332 185 528 3.96e-148 439
MS.gene53831.t1 MTR_7g015420 67.267 333 103 4 1 332 180 507 1.79e-147 444
MS.gene53831.t1 MTR_7g015310 66.667 333 99 5 2 332 186 508 4.31e-145 431
MS.gene53831.t1 MTR_7g015230 67.385 325 101 3 9 332 198 518 3.81e-144 429
MS.gene53831.t1 MTR_7g015620 64.201 338 110 6 1 332 180 512 2.80e-142 418
MS.gene53831.t1 MTR_7g015240 67.568 333 97 4 1 332 183 505 3.89e-142 422
MS.gene53831.t1 MTR_7g015280 64.653 331 100 4 2 332 185 498 1.83e-140 419
MS.gene53831.t1 MTR_7g015250 58.841 345 102 8 4 332 182 502 1.22e-119 366
MS.gene53831.t1 MTR_6g015805 35.821 335 196 12 4 329 176 500 4.37e-50 179
MS.gene53831.t1 MTR_4g111925 39.007 282 147 9 8 278 184 451 3.47e-46 166
MS.gene53831.t1 MTR_7g073660 39.007 282 147 9 8 278 184 451 5.08e-46 167
MS.gene53831.t1 MTR_6g015795 38.132 257 146 10 7 256 182 432 2.28e-42 155
MS.gene53831.t1 MTR_5g047060 35.197 304 165 10 8 296 186 472 6.60e-38 144
MS.gene53831.t1 MTR_5g047110 35.099 302 168 9 8 296 185 471 1.48e-37 142
MS.gene53831.t1 MTR_1g080740 34.470 264 140 11 2 258 170 407 1.09e-35 137
MS.gene53831.t1 MTR_7g115300 32.955 264 143 9 2 258 181 417 4.29e-34 132
MS.gene53831.t1 MTR_5g047120 31.290 310 171 13 8 306 191 469 1.90e-29 119
MS.gene53831.t1 MTR_2g080220 29.874 318 189 11 4 319 167 452 1.58e-25 107
MS.gene53831.t1 MTR_5g047070 31.518 257 136 11 61 306 1 228 1.83e-23 101
MS.gene53831.t1 MTR_2g030310 28.125 320 189 11 8 320 186 471 1.29e-21 96.3
MS.gene53831.t1 MTR_2g030310 28.125 320 189 11 8 320 186 471 1.43e-21 96.3
MS.gene53831.t1 MTR_4g061833 28.013 307 188 10 4 307 20 296 5.58e-19 88.2
MS.gene53831.t1 MTR_4g061833 28.013 307 188 10 4 307 171 447 1.11e-18 87.4
MS.gene53831.t1 MTR_4g061930 28.013 307 188 10 4 307 171 447 1.11e-18 87.4
MS.gene53831.t1 MTR_8g037700 28.800 250 151 8 9 253 203 430 1.99e-18 86.7
MS.gene53831.t1 MTR_4g109010 28.425 292 177 11 8 292 194 460 3.66e-17 83.2
MS.gene53831.t1 MTR_8g467150 27.305 282 148 11 10 289 177 403 2.46e-16 80.5
MS.gene53831.t1 MTR_6g048090 27.879 330 178 17 4 319 168 451 4.42e-16 79.7
MS.gene53831.t1 MTR_2g096160 27.778 252 149 10 9 254 174 398 5.22e-14 73.6
MS.gene53831.t1 MTR_1g040077 26.545 275 168 14 53 314 164 417 3.26e-13 70.9
MS.gene53831.t1 MTR_1g040073 26.545 275 168 14 53 314 164 417 5.60e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53831.t1 AT3G51550 37.987 308 172 7 8 310 184 477 1.76e-50 180
MS.gene53831.t1 AT5G39000 36.964 303 163 13 9 300 179 464 2.52e-44 162
MS.gene53831.t1 AT5G39020 35.625 320 183 10 9 320 176 480 1.27e-42 157
MS.gene53831.t1 AT3G04690 32.298 322 180 14 8 319 175 468 7.76e-40 149
MS.gene53831.t1 AT5G38990 34.862 327 198 9 9 329 173 490 1.22e-39 149
MS.gene53831.t1 AT5G28680 31.366 322 186 11 8 319 176 472 4.24e-39 147
MS.gene53831.t1 AT5G28680 31.366 322 186 11 8 319 176 472 4.24e-39 147
MS.gene53831.t1 AT5G39030 30.816 331 197 11 4 320 164 476 1.49e-33 131
MS.gene53831.t1 AT5G39030 30.816 331 197 11 4 320 170 482 1.81e-33 131
MS.gene53831.t1 AT1G30570 28.205 312 179 11 8 310 188 463 1.16e-24 105
MS.gene53831.t1 AT5G54380 29.114 316 189 13 4 313 167 453 2.46e-22 98.6
MS.gene53831.t1 AT5G59700 27.974 311 186 12 4 309 158 435 7.50e-22 97.1
MS.gene53831.t1 AT4G00300 25.538 325 209 14 7 324 172 470 2.96e-20 91.7
MS.gene53831.t1 AT2G21480 30.364 247 148 8 9 253 200 424 7.93e-18 85.1
MS.gene53831.t1 AT4G39110 29.555 247 150 8 9 253 201 425 2.71e-17 83.6
MS.gene53831.t1 AT5G61350 27.063 303 187 10 9 305 177 451 8.35e-17 82.0
MS.gene53831.t1 AT3G46290 26.693 251 159 10 4 251 159 387 1.32e-16 81.3
MS.gene53831.t1 AT2G23200 25.460 326 166 14 9 319 180 443 5.85e-11 63.9

Find 42 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGTTAAGTATACACTTTC+TGG 0.122965 7.4:+83928226 None:intergenic
AGTGATTTCAAATGATAATT+TGG 0.149454 7.4:+83928478 None:intergenic
CTTTCTGGTGCAGTGTAATT+TGG 0.155825 7.4:+83928241 None:intergenic
ATACAGGTATGTTTCGTAAT+TGG 0.186187 7.4:-83928357 MS.gene53831:CDS
ATGGGAGATATAATGGGTTT+TGG 0.274254 7.4:+83927803 None:intergenic
GTTCATTATGCTTTCATTAA+CGG 0.297513 7.4:-83928553 MS.gene53831:CDS
GTGATTTCAAATGATAATTT+GGG 0.348785 7.4:+83928479 None:intergenic
TACAGGTATGTTTCGTAATT+GGG 0.355748 7.4:-83928356 MS.gene53831:CDS
CAAATGCTGATGTGATTTAC+TGG 0.363866 7.4:-83928033 MS.gene53831:CDS
TTTGTGACGACTCTTATCTA+TGG 0.390930 7.4:+83927576 None:intergenic
AAGCATAATGAACATTTGAT+TGG 0.391835 7.4:+83928563 None:intergenic
TATCGTGATGTTACGTTGAA+TGG 0.404537 7.4:-83927878 MS.gene53831:CDS
ATCTAAATCTCATATTGAAA+TGG 0.404628 7.4:-83927648 MS.gene53831:CDS
TATCGTGTGTGCATAGTATC+TGG 0.414620 7.4:+83927899 None:intergenic
TACTTAACTGCAAGAAGTTA+TGG 0.421821 7.4:-83928214 MS.gene53831:CDS
AATTCATAACGACAAAACTT+TGG 0.422997 7.4:-83928431 MS.gene53831:CDS
ACAACATAATCCTTATGAAC+AGG 0.429030 7.4:+83927992 None:intergenic
ATACCTGTATCATTACTTGT+TGG 0.432515 7.4:+83928370 None:intergenic
AGCATCTATTGTTGGAATGA+TGG 0.437108 7.4:-83927693 MS.gene53831:CDS
ATACTGTTTCTCCAAGTAAC+GGG 0.443500 7.4:+83928321 None:intergenic
CTCATGCTAGCATCTATTGT+TGG 0.449185 7.4:-83927701 MS.gene53831:CDS
CAGTAAATCACATCAGCATT+TGG 0.449717 7.4:+83928034 None:intergenic
CAAAAGATTACAATGTAACT+TGG 0.455335 7.4:-83928180 MS.gene53831:CDS
TCGCATGGTTCCTGTTCATA+AGG 0.471594 7.4:-83928002 MS.gene53831:CDS
TTTGTGTAATAGAGAAAAGA+AGG 0.472288 7.4:+83928511 None:intergenic
GTTCCAACAAGTAATGATAC+AGG 0.482416 7.4:-83928373 MS.gene53831:CDS
TTGTGACGACTCTTATCTAT+GGG 0.493679 7.4:+83927577 None:intergenic
GATTGGCATATCAAGAATCC+AGG 0.523351 7.4:-83928100 MS.gene53831:CDS
AAAACCCATTATATCTCCCA+TGG 0.558851 7.4:-83927801 MS.gene53831:CDS
AAGTCACTTGAGACACTAAG+TGG 0.568622 7.4:+83928298 None:intergenic
ATACTTTATAGAATCAATGT+TGG 0.577641 7.4:-83928406 MS.gene53831:CDS
GGAAGACAAAGAACGGAGGT+TGG 0.578619 7.4:-83927613 MS.gene53831:CDS
TTACTGGAGTGGTGCTCGCA+TGG 0.583742 7.4:-83928017 MS.gene53831:CDS
GATACTGTTTCTCCAAGTAA+CGG 0.598041 7.4:+83928320 None:intergenic
TTGAAATGGAAGCAATGTCG+TGG 0.624408 7.4:-83927634 MS.gene53831:CDS
ATGTCGTGGAAGACAAAGAA+CGG 0.629191 7.4:-83927620 MS.gene53831:CDS
ATCACTTGACTTAGACAACA+CGG 0.649205 7.4:-83928461 MS.gene53831:CDS
TACTGTTTCTCCAAGTAACG+GGG 0.677122 7.4:+83928322 None:intergenic
AAAGTCAAAGAAATACACGT+TGG 0.685933 7.4:-83927753 MS.gene53831:CDS
AATGATAATTTGGGTCACTG+AGG 0.688896 7.4:+83928488 None:intergenic
GCTGATGTGATTTACTGGAG+TGG 0.708071 7.4:-83928028 MS.gene53831:CDS
TCGTGGAAGACAAAGAACGG+AGG 0.774729 7.4:-83927617 MS.gene53831:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAAAAATAATAATCTATT+TGG - chr7.4:83928324-83928343 MS.gene53831:CDS 10.0%
!! AGTGATTTCAAATGATAATT+TGG + chr7.4:83927682-83927701 None:intergenic 20.0%
!! ATACTTTATAGAATCAATGT+TGG - chr7.4:83927751-83927770 MS.gene53831:CDS 20.0%
!! ATCTAAATCTCATATTGAAA+TGG - chr7.4:83928509-83928528 MS.gene53831:CDS 20.0%
!! GTGATTTCAAATGATAATTT+GGG + chr7.4:83927681-83927700 None:intergenic 20.0%
!!! GATTTTTATTGATGATATCT+TGG - chr7.4:83928095-83928114 MS.gene53831:CDS 20.0%
! AAGCATAATGAACATTTGAT+TGG + chr7.4:83927597-83927616 None:intergenic 25.0%
! AATTCATAACGACAAAACTT+TGG - chr7.4:83927726-83927745 MS.gene53831:CDS 25.0%
! CAAAAGATTACAATGTAACT+TGG - chr7.4:83927977-83927996 MS.gene53831:CDS 25.0%
! GTTCATTATGCTTTCATTAA+CGG - chr7.4:83927604-83927623 MS.gene53831:CDS 25.0%
! TTTGTGTAATAGAGAAAAGA+AGG + chr7.4:83927649-83927668 None:intergenic 25.0%
!! AGTTACATTGTAATCTTTTG+TGG + chr7.4:83927977-83927996 None:intergenic 25.0%
!!! AAGTTTTGTCGTTATGAATT+TGG + chr7.4:83927726-83927745 None:intergenic 25.0%
!!! TATTTCTTTGACTTTTGAGT+TGG + chr7.4:83928399-83928418 None:intergenic 25.0%
!!! TGATGTTGCTTTTACTTATA+TGG - chr7.4:83928011-83928030 MS.gene53831:CDS 25.0%
!!! TTGCTTTTACTTATATGGTA+AGG - chr7.4:83928016-83928035 MS.gene53831:CDS 25.0%
AAAGTCAAAGAAATACACGT+TGG - chr7.4:83928404-83928423 MS.gene53831:CDS 30.0%
ACAACATAATCCTTATGAAC+AGG + chr7.4:83928168-83928187 None:intergenic 30.0%
ATACAGGTATGTTTCGTAAT+TGG - chr7.4:83927800-83927819 MS.gene53831:CDS 30.0%
ATACCTGTATCATTACTTGT+TGG + chr7.4:83927790-83927809 None:intergenic 30.0%
TACAGGTATGTTTCGTAATT+GGG - chr7.4:83927801-83927820 MS.gene53831:CDS 30.0%
TACTTAACTGCAAGAAGTTA+TGG - chr7.4:83927943-83927962 MS.gene53831:CDS 30.0%
! TACATTTTTGCGAGTTTGAT+TGG - chr7.4:83928040-83928059 MS.gene53831:CDS 30.0%
! TTTTCCATGGGAGATATAAT+GGG + chr7.4:83928363-83928382 None:intergenic 30.0%
! TTTTTCCATGGGAGATATAA+TGG + chr7.4:83928364-83928383 None:intergenic 30.0%
!! ATCGGTAAAACTTTTTCCAT+GGG + chr7.4:83928375-83928394 None:intergenic 30.0%
!! TATCGGTAAAACTTTTTCCA+TGG + chr7.4:83928376-83928395 None:intergenic 30.0%
!!! TTTGACTTTTGAGTTGGTAT+CGG + chr7.4:83928393-83928412 None:intergenic 30.0%
AAAACCCATTATATCTCCCA+TGG - chr7.4:83928356-83928375 MS.gene53831:CDS 35.0%
AATGATAATTTGGGTCACTG+AGG + chr7.4:83927672-83927691 None:intergenic 35.0%
AGCATCTATTGTTGGAATGA+TGG - chr7.4:83928464-83928483 MS.gene53831:CDS 35.0%
ATCACTTGACTTAGACAACA+CGG - chr7.4:83927696-83927715 MS.gene53831:CDS 35.0%
ATCTGAAAAACTCTATCACC+TGG + chr7.4:83928078-83928097 None:intergenic 35.0%
CAAATGCTGATGTGATTTAC+TGG - chr7.4:83928124-83928143 MS.gene53831:CDS 35.0%
CAGTAAATCACATCAGCATT+TGG + chr7.4:83928126-83928145 None:intergenic 35.0%
GCAGTTAAGTATACACTTTC+TGG + chr7.4:83927934-83927953 None:intergenic 35.0%
GTTCCAACAAGTAATGATAC+AGG - chr7.4:83927784-83927803 MS.gene53831:CDS 35.0%
TATCGTGATGTTACGTTGAA+TGG - chr7.4:83928279-83928298 MS.gene53831:CDS 35.0%
! ATACTGTTTCTCCAAGTAAC+GGG + chr7.4:83927839-83927858 None:intergenic 35.0%
! GATACTGTTTCTCCAAGTAA+CGG + chr7.4:83927840-83927859 None:intergenic 35.0%
!!! ATGGGAGATATAATGGGTTT+TGG + chr7.4:83928357-83928376 None:intergenic 35.0%
!!! CACGTTGGTTATTTTTGTTC+TGG - chr7.4:83928419-83928438 MS.gene53831:CDS 35.0%
AAGTCACTTGAGACACTAAG+TGG + chr7.4:83927862-83927881 None:intergenic 40.0%
ATGTCGTGGAAGACAAAGAA+CGG - chr7.4:83928537-83928556 MS.gene53831:CDS 40.0%
CTCATGCTAGCATCTATTGT+TGG - chr7.4:83928456-83928475 MS.gene53831:CDS 40.0%
GATTGGCATATCAAGAATCC+AGG - chr7.4:83928057-83928076 MS.gene53831:CDS 40.0%
GTGAGTATGAGCTCAAAAAG+AGG - chr7.4:83928186-83928205 MS.gene53831:CDS 40.0%
TATCGTGTGTGCATAGTATC+TGG + chr7.4:83928261-83928280 None:intergenic 40.0%
TTGAAATGGAAGCAATGTCG+TGG - chr7.4:83928523-83928542 MS.gene53831:CDS 40.0%
! CTTTCTGGTGCAGTGTAATT+TGG + chr7.4:83927919-83927938 None:intergenic 40.0%
! TAACGATTTTCCCCGTTACT+TGG - chr7.4:83927825-83927844 MS.gene53831:CDS 40.0%
! TACTGTTTCTCCAAGTAACG+GGG + chr7.4:83927838-83927857 None:intergenic 40.0%
GCTGATGTGATTTACTGGAG+TGG - chr7.4:83928129-83928148 MS.gene53831:CDS 45.0%
TCGCATGGTTCCTGTTCATA+AGG - chr7.4:83928155-83928174 MS.gene53831:CDS 45.0%
GGAAGACAAAGAACGGAGGT+TGG - chr7.4:83928544-83928563 MS.gene53831:CDS 50.0%
TCGTGGAAGACAAAGAACGG+AGG - chr7.4:83928540-83928559 MS.gene53831:CDS 50.0%
TTACTGGAGTGGTGCTCGCA+TGG - chr7.4:83928140-83928159 MS.gene53831:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 83927589 83928590 83927589 ID=MS.gene53831
chr7.4 mRNA 83927589 83928590 83927589 ID=MS.gene53831.t1;Parent=MS.gene53831
chr7.4 exon 83927589 83928590 83927589 ID=MS.gene53831.t1.exon1;Parent=MS.gene53831.t1
chr7.4 CDS 83927589 83928590 83927589 ID=cds.MS.gene53831.t1;Parent=MS.gene53831.t1
Gene Sequence

>MS.gene53831

ATGAACCAATCAAATGTTCATTATGCTTTCATTAACGGAATCGAAATTGTGTCTATGCCTTCTTTTCTCTATTACACAAACCTCAGTGACCCAAATTATCATTTGAAATCACTTGACTTAGACAACACGGAATACCAAATTCATAACGACAAAACTTTGGAGATACTTTATAGAATCAATGTTGGTGATAATGAAGTTCCAACAAGTAATGATACAGGTATGTTTCGTAATTGGGATAACGATTTTCCCCGTTACTTGGAGAAACAGTATCCACTTAGTGTCTCAAGTGACTTTGCACATCATCTAAACTATTTAAACAATACAATTCCAAATTACACTGCACCAGAAAGTGTATACTTAACTGCAAGAAGTTATGGAATGTATGCCACAAAAGATTACAATGTAACTTGGAATTTTGAAATTGATGTTGCTTTTACTTATATGGTAAGGTTACATTTTTGCGAGTTTGATTGGCATATCAAGAATCCAGGTGATAGAGTTTTTCAGATTTTTATTGATGATATCTTGGCTGAACCAAATGCTGATGTGATTTACTGGAGTGGTGCTCGCATGGTTCCTGTTCATAAGGATTATGTTGTGAGTATGAGCTCAAAAAGAGGAAGCTCTCAAATCGAAAGAGTTAATCTTTCGATCAAGCTACAACGATTACCAGATACTATGCACACACGATATCGTGATGTTACGTTGAATGGAATCGAGATTTGCAAAACAAGTGATAAAAATAATAATCTATTTGGATCGAATCCAAAACCCATTATATCTCCCATGGAAAAAGTTTTACCGATACCAACTCAAAAGTCAAAGAAATACACGTTGGTTATTTTTGTTCTGGTTGTAGTTTCATGTCTCATGCTAGCATCTATTGTTGGAATGATGGTTTTTGCTCAAAGAAGAAGACAATCTAAATCTCATATTGAAATGGAAGCAATGTCGTGGAAGACAAAGAACGGAGGTTGGCAGTCTTTACAATCCCATAGATAA

Protein sequence

>MS.gene53831.t1

MNQSNVHYAFINGIEIVSMPSFLYYTNLSDPNYHLKSLDLDNTEYQIHNDKTLEILYRINVGDNEVPTSNDTGMFRNWDNDFPRYLEKQYPLSVSSDFAHHLNYLNNTIPNYTAPESVYLTARSYGMYATKDYNVTWNFEIDVAFTYMVRLHFCEFDWHIKNPGDRVFQIFIDDILAEPNADVIYWSGARMVPVHKDYVVSMSSKRGSSQIERVNLSIKLQRLPDTMHTRYRDVTLNGIEICKTSDKNNNLFGSNPKPIISPMEKVLPIPTQKSKKYTLVIFVLVVVSCLMLASIVGMMVFAQRRRQSKSHIEMEAMSWKTKNGGWQSLQSHR