Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54337.t1 | XP_003620963.1 | 99.7 | 361 | 1 | 0 | 1 | 361 | 1 | 361 | 2.50E-199 | 704.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54337.t1 | P52904 | 92.0 | 361 | 27 | 2 | 1 | 361 | 1 | 359 | 8.2e-183 | 641.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54337.t1 | B7FJJ4 | 99.7 | 361 | 1 | 0 | 1 | 361 | 1 | 361 | 1.8e-199 | 704.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene54337 | MS.gene013304 | PPI |
| MS.gene011262 | MS.gene54337 | PPI |
| MS.gene54337 | MS.gene07846 | PPI |
| MS.gene54337 | MS.gene049277 | PPI |
| MS.gene037762 | MS.gene54337 | PPI |
| MS.gene052712 | MS.gene54337 | PPI |
| MS.gene54337 | MS.gene06396 | PPI |
| MS.gene54337 | MS.gene073516 | PPI |
| MS.gene54337 | MS.gene054963 | PPI |
| MS.gene80759 | MS.gene54337 | PPI |
| MS.gene54337 | MS.gene81790 | PPI |
| MS.gene54337 | MS.gene53350 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54337.t1 | MTR_7g005380 | 99.723 | 361 | 1 | 0 | 1 | 361 | 1 | 361 | 0.0 | 737 |
| MS.gene54337.t1 | MTR_3g076630 | 41.104 | 326 | 188 | 1 | 27 | 352 | 82 | 403 | 3.49e-88 | 271 |
| MS.gene54337.t1 | MTR_4g021470 | 36.086 | 327 | 202 | 4 | 23 | 348 | 33 | 353 | 1.75e-68 | 219 |
| MS.gene54337.t1 | MTR_7g009250 | 93.243 | 74 | 5 | 0 | 175 | 248 | 42 | 115 | 4.72e-41 | 140 |
| MS.gene54337.t1 | MTR_7g009250 | 58.182 | 55 | 23 | 0 | 85 | 139 | 7 | 61 | 9.93e-12 | 62.0 |
| MS.gene54337.t1 | MTR_7g009290 | 66.667 | 69 | 20 | 1 | 132 | 200 | 9 | 74 | 7.90e-23 | 91.7 |
| MS.gene54337.t1 | MTR_7g009310 | 95.122 | 41 | 2 | 0 | 160 | 200 | 6 | 46 | 1.82e-22 | 90.5 |
| MS.gene54337.t1 | MTR_3g107740 | 27.468 | 233 | 142 | 8 | 66 | 290 | 421 | 634 | 3.92e-11 | 64.7 |
| MS.gene54337.t1 | MTR_8g068265 | 26.587 | 252 | 167 | 8 | 69 | 317 | 427 | 663 | 5.14e-11 | 64.3 |
| MS.gene54337.t1 | MTR_8g068300 | 26.587 | 252 | 167 | 8 | 69 | 317 | 427 | 663 | 5.23e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54337.t1 | AT5G50850 | 86.188 | 362 | 42 | 2 | 1 | 354 | 1 | 362 | 0.0 | 639 |
| MS.gene54337.t1 | AT1G30120 | 40.456 | 351 | 202 | 2 | 5 | 352 | 60 | 406 | 2.16e-90 | 277 |
| MS.gene54337.t1 | AT2G34590 | 42.042 | 333 | 185 | 2 | 22 | 352 | 80 | 406 | 1.98e-89 | 275 |
| MS.gene54337.t1 | AT1G55510 | 36.337 | 344 | 210 | 5 | 8 | 348 | 10 | 347 | 6.42e-70 | 223 |
| MS.gene54337.t1 | AT3G13450 | 35.224 | 335 | 210 | 4 | 15 | 348 | 25 | 353 | 1.59e-68 | 219 |
| MS.gene54337.t1 | AT4G15560 | 24.727 | 275 | 187 | 7 | 19 | 290 | 390 | 647 | 3.37e-12 | 68.2 |
| MS.gene54337.t1 | AT3G21500 | 26.549 | 226 | 151 | 6 | 68 | 290 | 392 | 605 | 3.16e-11 | 65.1 |
| MS.gene54337.t1 | AT3G21500 | 26.549 | 226 | 151 | 6 | 68 | 290 | 391 | 604 | 3.65e-11 | 65.1 |
| MS.gene54337.t1 | AT3G21500 | 26.549 | 226 | 151 | 6 | 68 | 290 | 392 | 605 | 4.10e-11 | 64.7 |
Find 97 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACAAAGGTTATTAATTTA+AGG | 0.162693 | 7.2:+93353133 | MS.gene54337:intron |
| AATATGCAGGCTGGGTTTAC+TGG | 0.232671 | 7.2:+93350259 | MS.gene54337:intron |
| ATGCCCTGGGTTGAAAGTTT+TGG | 0.247026 | 7.2:+93352283 | MS.gene54337:CDS |
| GTTTACTTAAAGCTGCTATA+AGG | 0.289090 | 7.2:+93352333 | MS.gene54337:CDS |
| TCTTGGTATGGCTCATGCCC+TGG | 0.309803 | 7.2:+93352269 | MS.gene54337:CDS |
| TCACCCATCAAGAAGACTTT+AGG | 0.312229 | 7.2:-93346741 | None:intergenic |
| TTAGATTTCCAAGGGACTAT+TGG | 0.314457 | 7.2:+93348618 | MS.gene54337:intron |
| GGAGTTGGTGCTGCTTACTA+TGG | 0.330288 | 7.2:+93350286 | MS.gene54337:CDS |
| CATGCCTTATGCAGCAAATC+TGG | 0.332687 | 7.2:+93353338 | MS.gene54337:CDS |
| TCAAGAACTTCGGCAGAAAC+AGG | 0.334442 | 7.2:-93352521 | None:intergenic |
| AATGGTTGGCTTTGCTCTAA+AGG | 0.349744 | 7.2:+93352728 | MS.gene54337:CDS |
| TCAAGGAAAACTACAGGATC+AGG | 0.356678 | 7.2:-93352359 | None:intergenic |
| CTTTCCAGATTTGCTGCATA+AGG | 0.357769 | 7.2:-93353342 | None:intergenic |
| CAGGCTGGGTTTACTGGGAT+TGG | 0.358479 | 7.2:+93350265 | MS.gene54337:intron |
| TCCGATGGGAAGGCAGAAAC+TGG | 0.372273 | 7.2:-93352546 | None:intergenic |
| TCTCTCTTCTTCCGCTAAAC+AGG | 0.373627 | 7.2:+93346582 | MS.gene54337:CDS |
| AATGTACCTATTGTATTTAG+AGG | 0.374366 | 7.2:+93352045 | MS.gene54337:CDS |
| GCACCGGTGGAGAGAATTGC+CGG | 0.381050 | 7.2:+93353303 | MS.gene54337:CDS |
| GGGTTTACTGGGATTGGAGT+TGG | 0.388394 | 7.2:+93350271 | MS.gene54337:CDS |
| AAGGAATCAATCAAGATGTT+AGG | 0.392916 | 7.2:+93346418 | None:intergenic |
| CGAGCATCTTCAGATGAATA+TGG | 0.394225 | 7.2:-93352308 | None:intergenic |
| TGCAGCAAATCTGGAAAGAT+TGG | 0.409248 | 7.2:+93353347 | MS.gene54337:CDS |
| TTATGCAGTGTTATGCATCT+TGG | 0.410920 | 7.2:+93352252 | MS.gene54337:intron |
| AATGGTGCTGCTGCTGGTGT+TGG | 0.415359 | 7.2:+93352072 | MS.gene54337:CDS |
| TCTGTCATCGAGGAGAGTTT+TGG | 0.424908 | 7.2:+93353270 | MS.gene54337:CDS |
| TTTCCTGACAGAAGCATTGA+TGG | 0.431540 | 7.2:-93353181 | None:intergenic |
| AAATCAAATTACATGTCTGC+TGG | 0.434500 | 7.2:+93352015 | MS.gene54337:CDS |
| TTTACTTAAAGCTGCTATAA+GGG | 0.435542 | 7.2:+93352334 | MS.gene54337:CDS |
| TTGGGTCCTCTAAATACAAT+AGG | 0.453035 | 7.2:-93352051 | None:intergenic |
| CGATCCTAAAGTCTTCTTGA+TGG | 0.454138 | 7.2:+93346737 | MS.gene54337:CDS |
| GGACCCAATGGTGCTGCTGC+TGG | 0.458395 | 7.2:+93352066 | MS.gene54337:CDS |
| TTCAACTGTCACCAGTCTGT+TGG | 0.461416 | 7.2:-93353205 | None:intergenic |
| CTTGGTATGGCTCATGCCCT+GGG | 0.468165 | 7.2:+93352270 | MS.gene54337:CDS |
| AATGTCATAAACTCCACAAC+AGG | 0.479865 | 7.2:-93350316 | None:intergenic |
| AAGGGACTATTGGAGAAGTA+TGG | 0.485928 | 7.2:+93348628 | MS.gene54337:CDS |
| ATATGCAGGCTGGGTTTACT+GGG | 0.487916 | 7.2:+93350260 | MS.gene54337:intron |
| CAGATTTGCTGCATAAGGCA+TGG | 0.501282 | 7.2:-93353337 | None:intergenic |
| GAGATGTCTGAATGCGGAAA+AGG | 0.501518 | 7.2:-93346563 | None:intergenic |
| GACATTTAACTTCTCCATGC+AGG | 0.502562 | 7.2:+93350333 | MS.gene54337:CDS |
| CAGTGTTATGCATCTTGGTA+TGG | 0.510583 | 7.2:+93352257 | MS.gene54337:intron |
| ACACCAGCAGCAGCACCATT+GGG | 0.513248 | 7.2:-93352069 | None:intergenic |
| GCCCCGGCAATTCTCTCCAC+CGG | 0.513647 | 7.2:-93353306 | None:intergenic |
| CCACCATCAATGCTTCTGTC+AGG | 0.514146 | 7.2:+93353178 | MS.gene54337:CDS |
| ATGCCACAGATCTGTGCCTA+TGG | 0.514735 | 7.2:+93353503 | MS.gene54337:CDS |
| CTTAACAGATTGAGAGAGAA+GGG | 0.517313 | 7.2:+93352676 | MS.gene54337:intron |
| ATTTAGGCAGTTGCAGCCAT+AGG | 0.522619 | 7.2:-93353519 | None:intergenic |
| CACCGGTGGAGAGAATTGCC+GGG | 0.523558 | 7.2:+93353304 | MS.gene54337:CDS |
| AACACCAGCAGCAGCACCAT+TGG | 0.529263 | 7.2:-93352070 | None:intergenic |
| TCCTTCAATTTAGATTTCCA+AGG | 0.535088 | 7.2:+93348609 | MS.gene54337:intron |
| GGAGTGTCGAGAACCCTCTC+AGG | 0.535813 | 7.2:-93348652 | None:intergenic |
| AGATTTGCTGCATAAGGCAT+GGG | 0.544500 | 7.2:-93353336 | None:intergenic |
| ATACTTCTCCAATAGTCCCT+TGG | 0.545557 | 7.2:-93348626 | None:intergenic |
| TCCAGTTTCTGCCTTCCCAT+CGG | 0.546005 | 7.2:+93352545 | MS.gene54337:CDS |
| GGGTTTCCTCAGCATGGTGT+TGG | 0.548315 | 7.2:+93353231 | MS.gene54337:CDS |
| TTATAGGTTGGAGAATATCA+AGG | 0.548317 | 7.2:+93348483 | MS.gene54337:intron |
| AGAATATCAAGGTGCATACA+AGG | 0.548665 | 7.2:+93348494 | MS.gene54337:CDS |
| GGAAAGATTGGCTGTCCCAC+AGG | 0.549518 | 7.2:+93353359 | MS.gene54337:CDS |
| GATCCTAAAGTCTTCTTGAT+GGG | 0.550617 | 7.2:+93346738 | MS.gene54337:CDS |
| CAACAAACAAACCTGTTTAG+CGG | 0.555702 | 7.2:-93346593 | None:intergenic |
| AGACTGGTGACAGTTGAAGA+AGG | 0.558832 | 7.2:+93353210 | MS.gene54337:CDS |
| GCTTAACAGATTGAGAGAGA+AGG | 0.566225 | 7.2:+93352675 | MS.gene54337:intron |
| CCTTCAATTTAGATTTCCAA+GGG | 0.567285 | 7.2:+93348610 | MS.gene54337:intron |
| AATCAAATTACATGTCTGCT+GGG | 0.569255 | 7.2:+93352016 | MS.gene54337:CDS |
| AGTCTTCTTGATGGGTGAAG+AGG | 0.572562 | 7.2:+93346746 | MS.gene54337:CDS |
| CTATGGTCTAAAGCCTGTTG+TGG | 0.572678 | 7.2:+93350303 | MS.gene54337:CDS |
| CAAGAACTTCGGCAGAAACA+GGG | 0.590845 | 7.2:-93352520 | None:intergenic |
| GAAACTGGAATCAAGAACTT+CGG | 0.593707 | 7.2:-93352531 | None:intergenic |
| GTTAGGTGTTATAAGAAACA+AGG | 0.596856 | 7.2:+93346435 | MS.gene54337:CDS |
| CCTGACAGAAGCATTGATGG+TGG | 0.602274 | 7.2:-93353178 | None:intergenic |
| GTGCCAAAACTTTCAACCCA+GGG | 0.603446 | 7.2:-93352286 | None:intergenic |
| CAAGGAAAACTACAGGATCA+GGG | 0.604349 | 7.2:-93352358 | None:intergenic |
| GACTGGTGACAGTTGAAGAA+GGG | 0.606152 | 7.2:+93353211 | MS.gene54337:CDS |
| GAGATAAACACTAACCTGCA+TGG | 0.607390 | 7.2:-93350347 | None:intergenic |
| TGTCAGGAAAACCAACAGAC+TGG | 0.614276 | 7.2:+93353194 | MS.gene54337:CDS |
| CAGCCATAGGCACAGATCTG+TGG | 0.618473 | 7.2:-93353506 | None:intergenic |
| AGGCATGGGAACATCAGCCC+CGG | 0.619316 | 7.2:-93353322 | None:intergenic |
| GGAGAAGTATGGTCCTGAGA+GGG | 0.619724 | 7.2:+93348639 | MS.gene54337:CDS |
| ATTGTATTTAGAGGACCCAA+TGG | 0.620504 | 7.2:+93352054 | MS.gene54337:CDS |
| GAAGAAGGGTTTCCTCAGCA+TGG | 0.620750 | 7.2:+93353225 | MS.gene54337:CDS |
| GGTGCCAAAACTTTCAACCC+AGG | 0.622618 | 7.2:-93352287 | None:intergenic |
| CATCAAGAAGACTTTAGGAT+CGG | 0.623232 | 7.2:-93346736 | None:intergenic |
| CCTTCCCATCGGAAAAGCAA+AGG | 0.627357 | 7.2:+93352556 | MS.gene54337:CDS |
| GCTGAGACACTAGCAAAGGA+AGG | 0.630656 | 7.2:+93353011 | MS.gene54337:CDS |
| GGCTGCTGAGACACTAGCAA+AGG | 0.630698 | 7.2:+93353007 | MS.gene54337:intron |
| TTGATGGTGGCTCGATCAAG+CGG | 0.636023 | 7.2:-93353165 | None:intergenic |
| TGGTGGCTCGATCAAGCGGT+CGG | 0.637314 | 7.2:-93353161 | None:intergenic |
| CAAGAAGACTTTAGGATCGG+CGG | 0.641433 | 7.2:-93346733 | None:intergenic |
| TGGCTATCTTGATGCACCGG+TGG | 0.641507 | 7.2:+93353290 | MS.gene54337:CDS |
| ACCGGTGGAGAGAATTGCCG+GGG | 0.643645 | 7.2:+93353305 | MS.gene54337:CDS |
| TATTCATCTGAAGATGCTCG+TGG | 0.654209 | 7.2:+93352311 | MS.gene54337:CDS |
| AAAGGAAGGAATCAGTGCTG+AGG | 0.656553 | 7.2:+93353025 | MS.gene54337:CDS |
| TGGAGAAGTATGGTCCTGAG+AGG | 0.669482 | 7.2:+93348638 | MS.gene54337:CDS |
| TGTCTGAATGCGGAAAAGGA+AGG | 0.671745 | 7.2:-93346559 | None:intergenic |
| TCAGCGCCAACACCATGCTG+AGG | 0.686283 | 7.2:-93353237 | None:intergenic |
| AGAAGAGAGATGTCTGAATG+CGG | 0.692625 | 7.2:-93346569 | None:intergenic |
| AATCTGTGCATCTGTCATCG+AGG | 0.746298 | 7.2:+93353260 | MS.gene54337:CDS |
| TCTCGACACTCCCATCACTG+AGG | 0.757157 | 7.2:+93348663 | MS.gene54337:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 93346433 | 93353539 | 93346433 | ID=MS.gene54337 |
| chr7.2 | mRNA | 93346433 | 93353539 | 93346433 | ID=MS.gene54337.t1;Parent=MS.gene54337 |
| chr7.2 | exon | 93346433 | 93346456 | 93346433 | ID=MS.gene54337.t1.exon1;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93346433 | 93346456 | 93346433 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93346547 | 93346603 | 93346547 | ID=MS.gene54337.t1.exon2;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93346547 | 93346603 | 93346547 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93346687 | 93346767 | 93346687 | ID=MS.gene54337.t1.exon3;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93346687 | 93346767 | 93346687 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93348489 | 93348515 | 93348489 | ID=MS.gene54337.t1.exon4;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93348489 | 93348515 | 93348489 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93348622 | 93348684 | 93348622 | ID=MS.gene54337.t1.exon5;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93348622 | 93348684 | 93348622 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93350268 | 93350354 | 93350268 | ID=MS.gene54337.t1.exon6;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93350268 | 93350354 | 93350268 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93351985 | 93352110 | 93351985 | ID=MS.gene54337.t1.exon7;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93351985 | 93352110 | 93351985 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93352260 | 93352393 | 93352260 | ID=MS.gene54337.t1.exon8;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93352260 | 93352393 | 93352260 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93352505 | 93352577 | 93352505 | ID=MS.gene54337.t1.exon9;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93352505 | 93352577 | 93352505 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93352684 | 93352749 | 93352684 | ID=MS.gene54337.t1.exon10;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93352684 | 93352749 | 93352684 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93353008 | 93353046 | 93353008 | ID=MS.gene54337.t1.exon11;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93353008 | 93353046 | 93353008 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93353141 | 93353380 | 93353141 | ID=MS.gene54337.t1.exon12;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93353141 | 93353380 | 93353141 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| chr7.2 | exon | 93353471 | 93353539 | 93353471 | ID=MS.gene54337.t1.exon13;Parent=MS.gene54337.t1 |
| chr7.2 | CDS | 93353471 | 93353539 | 93353471 | ID=cds.MS.gene54337.t1;Parent=MS.gene54337.t1 |
| Gene Sequence |
| Protein sequence |
