Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54480.t1 | XP_003626514.1 | 93.6 | 297 | 14 | 1 | 9 | 305 | 2 | 293 | 5.90E-149 | 537 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54480.t1 | Q8S8M5 | 58.4 | 303 | 109 | 4 | 4 | 305 | 12 | 298 | 3.5e-86 | 319.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54480.t1 | G7L308 | 93.6 | 297 | 14 | 1 | 9 | 305 | 2 | 293 | 4.2e-149 | 537.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene54480 | MS.gene06902 | PPI |
| MS.gene54480 | MS.gene013576 | PPI |
| MS.gene31641 | MS.gene54480 | PPI |
| MS.gene54480 | MS.gene021449 | PPI |
| MS.gene057119 | MS.gene54480 | PPI |
| MS.gene059163 | MS.gene54480 | PPI |
| MS.gene06693 | MS.gene54480 | PPI |
| MS.gene059164 | MS.gene54480 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54480.t1 | MTR_7g116710 | 93.603 | 297 | 14 | 1 | 9 | 305 | 2 | 293 | 0.0 | 573 |
| MS.gene54480.t1 | MTR_8g028410 | 69.868 | 302 | 85 | 2 | 4 | 305 | 15 | 310 | 1.24e-149 | 422 |
| MS.gene54480.t1 | MTR_8g028410 | 73.200 | 250 | 61 | 2 | 4 | 253 | 15 | 258 | 1.84e-127 | 365 |
| MS.gene54480.t1 | MTR_8g028410 | 76.056 | 213 | 45 | 2 | 4 | 216 | 15 | 221 | 1.37e-112 | 325 |
| MS.gene54480.t1 | MTR_8g028410 | 83.060 | 183 | 31 | 0 | 4 | 186 | 15 | 197 | 7.30e-110 | 317 |
| MS.gene54480.t1 | MTR_1g007880 | 49.242 | 132 | 67 | 0 | 3 | 134 | 12 | 143 | 8.09e-39 | 139 |
| MS.gene54480.t1 | MTR_1g007880 | 49.242 | 132 | 67 | 0 | 3 | 134 | 31 | 162 | 1.71e-38 | 139 |
| MS.gene54480.t1 | MTR_3g091760 | 48.855 | 131 | 67 | 0 | 4 | 134 | 16 | 146 | 1.35e-37 | 134 |
| MS.gene54480.t1 | MTR_3g091760 | 48.855 | 131 | 67 | 0 | 4 | 134 | 16 | 146 | 2.89e-37 | 135 |
| MS.gene54480.t1 | MTR_6g087680 | 39.516 | 124 | 74 | 1 | 9 | 132 | 16 | 138 | 3.48e-24 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54480.t1 | AT2G46225 | 58.416 | 303 | 109 | 4 | 4 | 305 | 12 | 298 | 1.10e-117 | 340 |
| MS.gene54480.t1 | AT2G46225 | 53.293 | 334 | 108 | 5 | 4 | 305 | 12 | 329 | 1.45e-112 | 329 |
| MS.gene54480.t1 | AT2G46225 | 56.436 | 303 | 103 | 5 | 4 | 305 | 12 | 286 | 3.45e-108 | 316 |
| MS.gene54480.t1 | AT3G49290 | 50.382 | 131 | 65 | 0 | 4 | 134 | 7 | 137 | 2.61e-39 | 140 |
| MS.gene54480.t1 | AT3G49290 | 50.382 | 131 | 65 | 0 | 4 | 134 | 7 | 137 | 2.61e-39 | 140 |
| MS.gene54480.t1 | AT5G24310 | 45.985 | 137 | 74 | 0 | 4 | 140 | 7 | 143 | 3.65e-37 | 132 |
| MS.gene54480.t1 | AT5G24310 | 45.985 | 137 | 74 | 0 | 4 | 140 | 13 | 149 | 4.92e-37 | 134 |
| MS.gene54480.t1 | AT5G24310 | 45.985 | 137 | 74 | 0 | 4 | 140 | 7 | 143 | 5.02e-37 | 134 |
| MS.gene54480.t1 | AT5G24310 | 45.985 | 137 | 74 | 0 | 4 | 140 | 13 | 149 | 5.72e-37 | 134 |
| MS.gene54480.t1 | AT5G42030 | 47.244 | 127 | 67 | 0 | 8 | 134 | 18 | 144 | 4.17e-35 | 126 |
| MS.gene54480.t1 | AT5G42030 | 47.244 | 127 | 67 | 0 | 8 | 134 | 18 | 144 | 7.23e-35 | 128 |
| MS.gene54480.t1 | AT5G42030 | 47.244 | 127 | 67 | 0 | 8 | 134 | 18 | 144 | 2.00e-33 | 125 |
| MS.gene54480.t1 | AT5G42030 | 44.578 | 83 | 46 | 0 | 52 | 134 | 15 | 97 | 2.86e-18 | 82.4 |
Find 56 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGGTTGAGGACGATTTC+TGG | 0.226935 | 7.4:-92005817 | None:intergenic |
| TTCAGACCATTGACAACATT+TGG | 0.289027 | 7.4:+92013840 | MS.gene54480:CDS |
| ACTAGATTCGGTCAATAAAA+AGG | 0.307426 | 7.4:+92005743 | MS.gene54480:intron |
| GAGCTTTGTCTTCGCCCTTC+AGG | 0.315784 | 7.4:+92004405 | MS.gene54480:CDS |
| CTGTCTGCAGGATGCATTAG+AGG | 0.336506 | 7.4:+92013815 | MS.gene54480:intron |
| CCAAATGGAGTGCCTACTTC+TGG | 0.348637 | 7.4:+92013575 | MS.gene54480:CDS |
| GTCAATGCTGTTGATCATCT+TGG | 0.348893 | 7.4:+92005335 | MS.gene54480:CDS |
| AAGAAGATCAGTTAACTTGC+AGG | 0.369447 | 7.4:-92005366 | None:intergenic |
| GACATTCGACGAGGTTTCAA+TGG | 0.383001 | 7.4:+92004372 | MS.gene54480:CDS |
| TCAATGCTGTTGATCATCTT+GGG | 0.401596 | 7.4:+92005336 | MS.gene54480:CDS |
| TTCCTGTGACACCGAGTGTT+TGG | 0.409346 | 7.4:-92013607 | None:intergenic |
| GTGGAAAACTCCAGTGGTCT+TGG | 0.420567 | 7.4:-92012841 | None:intergenic |
| CCTCTTTGTTTGTGTATACT+CGG | 0.427986 | 7.4:-92005551 | None:intergenic |
| TTTGCATCAGTCTGTTTGTA+TGG | 0.428398 | 7.4:-92005777 | None:intergenic |
| GAAGATCTGTACTCTAAATC+AGG | 0.431053 | 7.4:+92005424 | MS.gene54480:CDS |
| TCAGACCATTGACAACATTT+GGG | 0.437041 | 7.4:+92013841 | MS.gene54480:CDS |
| AAATTCTCTGCCAAGACCAC+TGG | 0.477014 | 7.4:+92012831 | MS.gene54480:CDS |
| CGTCCTTGGTTCTGCAAAAG+TGG | 0.480732 | 7.4:-92012806 | None:intergenic |
| CCAGAAGTAGGCACTCCATT+TGG | 0.490166 | 7.4:-92013575 | None:intergenic |
| TAGAGAGTAAGAGATGGAAT+TGG | 0.493594 | 7.4:+92004345 | None:intergenic |
| CATCAGTCTGTTTGTATGGA+CGG | 0.496392 | 7.4:-92005773 | None:intergenic |
| GGTTCGGCAACAGCAGTTGT+TGG | 0.504184 | 7.4:+92005575 | MS.gene54480:CDS |
| TCGAACAACCACGTACCAAA+TGG | 0.513608 | 7.4:+92013560 | MS.gene54480:CDS |
| AGAAATCGTCCTCAACCATC+AGG | 0.513700 | 7.4:+92005819 | MS.gene54480:CDS |
| TCTTCTTGAGCAGCAAACAT+TGG | 0.515387 | 7.4:+92005382 | MS.gene54480:CDS |
| TATTCAACAACAAACCTGTA+AGG | 0.519328 | 7.4:-92012863 | None:intergenic |
| AAGGACCCAAATGTTGTCAA+TGG | 0.520330 | 7.4:-92013846 | None:intergenic |
| AGCCAAACACTCGGTGTCAC+AGG | 0.523214 | 7.4:+92013605 | MS.gene54480:CDS |
| TGTGACACCGAGTGTTTGGC+TGG | 0.528799 | 7.4:-92013603 | None:intergenic |
| AGTACAGATCTTCAAGTCCA+TGG | 0.530996 | 7.4:-92005414 | None:intergenic |
| TTCAGGAACTTAAGAATCTG+AGG | 0.538349 | 7.4:+92004775 | MS.gene54480:intron |
| ATGTAGTGCTTGTGATGTCT+AGG | 0.552043 | 7.4:-92005606 | None:intergenic |
| ACGTGGTTGTTCGATGCTGA+AGG | 0.560331 | 7.4:-92013551 | None:intergenic |
| GGTGCTGCCAGCCAAACACT+CGG | 0.571525 | 7.4:+92013596 | MS.gene54480:CDS |
| AACTACACACCTGATGGTTG+AGG | 0.571872 | 7.4:-92005828 | None:intergenic |
| ATGTACATTTAGATTGTCAA+AGG | 0.575135 | 7.4:-92013865 | None:intergenic |
| ACATTTCTTTCTGATCACTA+CGG | 0.579231 | 7.4:-92004842 | None:intergenic |
| TGTCTGCAGGATGCATTAGA+GGG | 0.579552 | 7.4:+92013816 | MS.gene54480:intron |
| TGGCTGGCAGCACCAGAAGT+AGG | 0.581051 | 7.4:-92013587 | None:intergenic |
| AGATCTGCAAAATTGCGAGC+CGG | 0.582308 | 7.4:+92013948 | MS.gene54480:CDS |
| CGAGTATACACAAACAAAGA+GGG | 0.585087 | 7.4:+92005552 | MS.gene54480:CDS |
| AACATTGGATGTTTCAACCA+TGG | 0.594116 | 7.4:+92005397 | MS.gene54480:CDS |
| AAACACTCGGTGTCACAGGA+AGG | 0.599063 | 7.4:+92013609 | MS.gene54480:CDS |
| CCGAGTATACACAAACAAAG+AGG | 0.608727 | 7.4:+92005551 | MS.gene54480:CDS |
| CATGCCTGCAGATAATGAAG+AGG | 0.622838 | 7.4:+92013514 | MS.gene54480:intron |
| TCAACAACAAACCTGTAAGG+TGG | 0.623592 | 7.4:-92012860 | None:intergenic |
| CAGATAATGAAGAGGTTACA+CGG | 0.624605 | 7.4:+92013522 | MS.gene54480:intron |
| AGGTATTTATGAGACAGAGC+CGG | 0.627499 | 7.4:-92013967 | None:intergenic |
| AACACTCGGTGTCACAGGAA+GGG | 0.628984 | 7.4:+92013610 | MS.gene54480:CDS |
| TAGGCACTCCATTTGGTACG+TGG | 0.635524 | 7.4:-92013568 | None:intergenic |
| ATGAATGTGAGGTACCTGAA+GGG | 0.651539 | 7.4:-92004419 | None:intergenic |
| TCTGCAGCAGAATAGAGTTG+AGG | 0.670021 | 7.4:-92004798 | None:intergenic |
| GAGTATACACAAACAAAGAG+GGG | 0.680540 | 7.4:+92005553 | MS.gene54480:CDS |
| TGTAAGGTGGAAAACTCCAG+TGG | 0.700267 | 7.4:-92012847 | None:intergenic |
| AGTATACACAAACAAAGAGG+GGG | 0.716843 | 7.4:+92005554 | MS.gene54480:CDS |
| ATTGGCAATGACATTCGACG+AGG | 0.717511 | 7.4:+92004363 | MS.gene54480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 92004358 | 92013983 | 92004358 | ID=MS.gene54480 |
| chr7.4 | mRNA | 92004358 | 92013983 | 92004358 | ID=MS.gene54480.t1;Parent=MS.gene54480 |
| chr7.4 | exon | 92004358 | 92004426 | 92004358 | ID=MS.gene54480.t1.exon1;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92004358 | 92004426 | 92004358 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92004780 | 92004862 | 92004780 | ID=MS.gene54480.t1.exon2;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92004780 | 92004862 | 92004780 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92005295 | 92005445 | 92005295 | ID=MS.gene54480.t1.exon3;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92005295 | 92005445 | 92005295 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92005537 | 92005636 | 92005537 | ID=MS.gene54480.t1.exon4;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92005537 | 92005636 | 92005537 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92005748 | 92005840 | 92005748 | ID=MS.gene54480.t1.exon5;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92005748 | 92005840 | 92005748 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92005963 | 92006045 | 92005963 | ID=MS.gene54480.t1.exon6;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92005963 | 92006045 | 92005963 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92012798 | 92012870 | 92012798 | ID=MS.gene54480.t1.exon7;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92012798 | 92012870 | 92012798 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92013525 | 92013631 | 92013525 | ID=MS.gene54480.t1.exon8;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92013525 | 92013631 | 92013525 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| chr7.4 | exon | 92013825 | 92013983 | 92013825 | ID=MS.gene54480.t1.exon9;Parent=MS.gene54480.t1 |
| chr7.4 | CDS | 92013825 | 92013983 | 92013825 | ID=cds.MS.gene54480.t1;Parent=MS.gene54480.t1 |
| Gene Sequence |
| Protein sequence |
