AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54738


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54738.t1 MTR_6g008900 97.849 279 6 0 1 279 93 371 0.0 574
MS.gene54738.t1 MTR_4g071620 97.849 279 6 0 1 279 93 371 0.0 574
MS.gene54738.t1 MTR_5g036580 68.148 270 84 1 1 270 94 361 4.15e-139 397
MS.gene54738.t1 MTR_7g109210 65.672 268 83 3 1 268 93 351 2.07e-132 379
MS.gene54738.t1 MTR_3g092310 61.049 267 99 3 2 268 71 332 2.04e-122 353
MS.gene54738.t1 MTR_3g111600 61.423 267 97 2 1 267 12 272 4.88e-121 347
MS.gene54738.t1 MTR_7g094230 60.219 274 102 3 1 274 83 349 1.38e-120 349
MS.gene54738.t1 MTR_8g078240 59.176 267 105 1 1 267 74 336 1.03e-119 346
MS.gene54738.t1 MTR_1g021855 61.940 268 95 3 1 267 97 358 2.17e-119 346
MS.gene54738.t1 MTR_5g019220 59.176 267 107 1 1 267 94 358 8.04e-119 345
MS.gene54738.t1 MTR_4g118030 58.364 269 104 4 1 268 77 338 1.60e-115 336
MS.gene54738.t1 MTR_1g089820 60.432 278 100 5 1 278 86 353 1.10e-113 332
MS.gene54738.t1 MTR_2g461670 34.066 182 112 5 73 254 395 568 2.74e-24 102
MS.gene54738.t1 MTR_4g109890 32.836 201 123 7 73 272 340 529 9.41e-23 97.8
MS.gene54738.t1 MTR_2g015550 31.401 207 124 7 73 279 339 527 3.20e-21 93.2
MS.gene54738.t1 MTR_8g092000 32.000 175 111 5 73 247 384 550 1.07e-20 92.0
MS.gene54738.t1 MTR_7g055600 29.592 196 125 6 57 247 217 404 2.83e-20 90.1
MS.gene54738.t1 MTR_7g057530 29.592 196 125 6 57 247 217 404 2.83e-20 90.1
MS.gene54738.t1 MTR_7g075840 28.431 204 129 6 73 276 468 654 3.63e-19 87.4
MS.gene54738.t1 MTR_4g133570 30.000 200 122 8 73 265 261 449 5.34e-19 86.7
MS.gene54738.t1 MTR_8g033360 31.551 187 118 4 44 225 403 584 5.37e-19 87.0
MS.gene54738.t1 MTR_6g016380 30.857 175 113 5 73 247 359 525 7.18e-19 86.7
MS.gene54738.t1 MTR_3g107930 28.302 212 139 5 44 250 358 561 1.54e-18 85.5
MS.gene54738.t1 MTR_7g012370 28.729 181 116 6 73 247 334 507 1.80e-18 85.5
MS.gene54738.t1 MTR_3g107930 28.302 212 139 5 44 250 435 638 1.95e-18 85.5
MS.gene54738.t1 MTR_2g027740 30.337 178 110 5 73 247 471 637 1.80e-17 82.4
MS.gene54738.t1 MTR_2g082650 28.643 199 126 8 73 265 209 397 2.88e-17 81.3
MS.gene54738.t1 MTR_2g082650 28.643 199 126 8 73 265 338 526 5.32e-17 80.9
MS.gene54738.t1 MTR_3g452360 28.272 191 125 6 64 247 166 351 9.62e-15 73.9
MS.gene54738.t1 MTR_3g452360 28.272 191 125 6 64 247 315 500 1.89e-14 73.6
MS.gene54738.t1 MTR_7g096160 25.714 175 125 3 73 247 455 624 5.00e-14 72.4
MS.gene54738.t1 MTR_6g013840 28.859 149 98 4 103 247 358 502 9.18e-13 68.6
MS.gene54738.t1 MTR_7g074680 31.325 166 93 9 103 265 403 550 4.94e-11 63.2
MS.gene54738.t1 MTR_7g074680 31.325 166 93 9 103 265 443 590 5.09e-11 63.2
MS.gene54738.t1 MTR_7g074680 31.325 166 93 9 103 265 463 610 5.60e-11 63.2
MS.gene54738.t1 MTR_7g074680 31.325 166 93 9 103 265 463 610 5.71e-11 63.2
MS.gene54738.t1 MTR_7g074680 31.325 166 93 9 103 265 500 647 5.83e-11 63.2
MS.gene54738.t1 MTR_7g074680 31.325 166 93 9 103 265 483 630 5.99e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54738.t1 AT3G28340 66.909 275 85 3 1 274 86 355 6.62e-142 404
MS.gene54738.t1 AT1G70090 67.910 268 84 1 1 268 97 362 3.11e-139 398
MS.gene54738.t1 AT1G70090 67.910 268 84 1 1 268 97 362 3.11e-139 398
MS.gene54738.t1 AT1G24170 67.164 268 86 1 1 268 100 365 6.05e-137 392
MS.gene54738.t1 AT3G62660 67.164 268 79 3 1 268 98 356 2.70e-132 379
MS.gene54738.t1 AT4G02130 66.418 268 84 3 1 268 81 342 4.07e-131 375
MS.gene54738.t1 AT4G02130 66.418 268 84 3 1 268 81 342 4.07e-131 375
MS.gene54738.t1 AT4G02130 66.418 268 84 3 1 268 81 342 4.07e-131 375
MS.gene54738.t1 AT1G02720 63.197 269 91 5 1 268 96 357 1.38e-123 357
MS.gene54738.t1 AT1G02720 63.197 269 91 5 1 268 96 357 1.38e-123 357
MS.gene54738.t1 AT3G06260 61.940 268 96 5 1 268 81 342 5.44e-122 352
MS.gene54738.t1 AT3G50760 59.259 270 101 3 1 267 68 331 1.75e-116 338
MS.gene54738.t1 AT1G13250 61.194 268 98 3 1 268 75 336 3.56e-112 327
MS.gene54738.t1 AT1G19300 62.547 267 96 2 1 267 80 342 9.11e-112 327
MS.gene54738.t1 AT1G18580 29.078 282 157 11 4 254 246 515 2.31e-25 105
MS.gene54738.t1 AT3G25140 30.435 207 125 7 48 247 335 529 1.04e-21 94.7
MS.gene54738.t1 AT4G38270 31.429 175 112 4 73 247 480 646 1.53e-20 91.7
MS.gene54738.t1 AT4G38270 31.429 175 112 4 73 247 484 650 1.65e-20 91.7
MS.gene54738.t1 AT3G61130 29.487 234 143 8 28 250 424 646 5.49e-20 90.1
MS.gene54738.t1 AT5G47780 32.000 175 111 5 73 247 420 586 6.66e-20 89.7
MS.gene54738.t1 AT3G01040 27.513 189 126 6 73 255 329 512 4.37e-19 87.0
MS.gene54738.t1 AT3G01040 27.513 189 126 6 73 255 329 512 4.37e-19 87.0
MS.gene54738.t1 AT3G01040 27.513 189 126 6 73 255 296 479 4.38e-19 87.0
MS.gene54738.t1 AT3G58790 29.050 179 119 5 73 247 204 378 2.62e-18 84.3
MS.gene54738.t1 AT3G58790 29.442 197 128 7 73 265 336 525 3.76e-18 84.3
MS.gene54738.t1 AT5G15470 27.749 191 123 7 73 255 329 512 8.75e-18 83.2
MS.gene54738.t1 AT5G54690 27.374 179 121 5 73 247 331 504 1.47e-17 82.8
MS.gene54738.t1 AT3G02350 31.579 209 129 6 44 247 332 531 2.11e-17 82.4
MS.gene54738.t1 AT2G30575 30.769 195 122 6 55 247 397 580 8.11e-17 80.5
MS.gene54738.t1 AT2G46480 30.337 178 116 5 73 250 344 513 1.62e-16 79.7
MS.gene54738.t1 AT2G46480 30.337 178 116 5 73 250 357 526 1.71e-16 79.7
MS.gene54738.t1 AT1G06780 29.944 177 115 5 73 247 403 572 1.35e-15 77.0
MS.gene54738.t1 AT1G06780 29.944 177 115 5 73 247 390 559 1.54e-15 76.6
MS.gene54738.t1 AT2G20810 35.393 178 106 5 73 250 342 510 1.87e-15 76.6

Find 43 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGATATTCAAGATTTA+TGG 0.160378 6.2:-4476127 MS.gene54738:CDS
TATATGCCTCATGTGGGATA+TGG 0.303444 6.2:-4475627 MS.gene54738:CDS
CTCCAATGTAACAAACTAAC+TGG 0.371144 6.2:+4475729 None:intergenic
TGTTACATTGGAGTGGTAAA+GGG 0.388590 6.2:-4475719 MS.gene54738:CDS
TCAAGTGCTTGTCTTATTGA+AGG 0.389187 6.2:+4476242 None:intergenic
TTGAAGCTATTGAACATAGA+TGG 0.398424 6.2:-4475812 MS.gene54738:CDS
AGAATAAAGATGAGTTTACT+AGG 0.399390 6.2:-4476343 MS.gene54738:CDS
GTGAGAAGGGTTTATAAGCT+TGG 0.416693 6.2:-4475873 MS.gene54738:CDS
TTGTTACATTGGAGTGGTAA+AGG 0.425011 6.2:-4475720 MS.gene54738:CDS
GTCCAGTTAGTTTGTTACAT+TGG 0.426248 6.2:-4475731 MS.gene54738:CDS
GAGTTTACTAGGATTGTTCA+TGG 0.433334 6.2:-4476332 MS.gene54738:CDS
AAATTTGCATGACAATATTC+CGG 0.448533 6.2:+4476068 None:intergenic
ACACAAAGAAGATTGAGAAA+TGG 0.449104 6.2:-4475911 MS.gene54738:CDS
TGGTAATGGATTTGGTGAAA+TGG 0.449678 6.2:-4475947 MS.gene54738:CDS
TTGACCGATTCGAAGGTAAT+CGG 0.457785 6.2:-4476095 MS.gene54738:CDS
CAACACTGGTGTGATGGTAA+TGG 0.479656 6.2:-4475961 MS.gene54738:CDS
TAAACTTTGAATTTCAATGA+AGG 0.482539 6.2:+4476302 None:intergenic
TAACTTCTTGCATAATTCAA+AGG 0.486802 6.2:+4476215 None:intergenic
TGGTGTGATGGTAATGGATT+TGG 0.491814 6.2:-4475955 MS.gene54738:CDS
TGAGGCATATAGAGATCATA+AGG 0.492254 6.2:+4475639 None:intergenic
AGATGGAATCAACATGGTCT+TGG 0.496429 6.2:-4475795 MS.gene54738:CDS
GAAATGGATGGAAATTCAAA+AGG 0.498984 6.2:-4475895 MS.gene54738:CDS
GGAAATTCAAAAGGTGAGAA+GGG 0.517101 6.2:-4475886 MS.gene54738:CDS
GGTTTCGTTGACCGATTCGA+AGG 0.525208 6.2:-4476102 MS.gene54738:CDS
ATTGCATCAAGTCTTCTCCA+TGG 0.528733 6.2:+4475693 None:intergenic
TGGAAATTCAAAAGGTGAGA+AGG 0.532785 6.2:-4475887 MS.gene54738:CDS
AGTTGTAGAAGTTTGCATCC+TGG 0.544023 6.2:-4475753 MS.gene54738:CDS
AAAGAAGATTGAGAAATGGA+TGG 0.561407 6.2:-4475907 MS.gene54738:CDS
TGATCTCTATATGCCTCATG+TGG 0.564137 6.2:-4475634 MS.gene54738:CDS
GTTAGTTTGTTACATTGGAG+TGG 0.566455 6.2:-4475726 MS.gene54738:CDS
TTCGAAGGTAATCGGTGCAC+CGG 0.567159 6.2:-4476087 MS.gene54738:CDS
ATGCCATGTTATTTCAACAC+TGG 0.573743 6.2:-4475975 MS.gene54738:CDS
TGGAATCAACATGGTCTTGG+TGG 0.582134 6.2:-4475792 MS.gene54738:CDS
TGCACCGATTACCTTCGAAT+CGG 0.583961 6.2:+4476091 None:intergenic
TGTAACAAACTAACTGGACC+AGG 0.606289 6.2:+4475735 None:intergenic
TGATATTCAAGATTTATGGA+AGG 0.616697 6.2:-4476123 MS.gene54738:CDS
TTATTTCAACACTGGTGTGA+TGG 0.626622 6.2:-4475967 MS.gene54738:CDS
GAATGTCCATATCCCACATG+AGG 0.636097 6.2:+4475621 None:intergenic
ACACCAGTGTTGAAATAACA+TGG 0.638418 6.2:+4475972 None:intergenic
GATTTGGTGAAATGGAGAGA+AGG 0.660269 6.2:-4475939 MS.gene54738:CDS
GGAGTGGTAAAGGGAAACCA+TGG 0.670933 6.2:-4475710 MS.gene54738:CDS
GATCTCTATATGCCTCATGT+GGG 0.673689 6.2:-4475633 MS.gene54738:CDS
GAACATAGATGGAATCAACA+TGG 0.707717 6.2:-4475801 MS.gene54738:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAAAAAACAAATTCTT+AGG + chr6.2:4475653-4475672 None:intergenic 15.0%
!! TAAACTTTGAATTTCAATGA+AGG + chr6.2:4475740-4475759 None:intergenic 20.0%
!!! TTGATGATATTCAAGATTTA+TGG - chr6.2:4475912-4475931 MS.gene54738:CDS 20.0%
! AAATTTGCATGACAATATTC+CGG + chr6.2:4475974-4475993 None:intergenic 25.0%
! AGAATAAAGATGAGTTTACT+AGG - chr6.2:4475696-4475715 MS.gene54738:CDS 25.0%
! TAACTTCTTGCATAATTCAA+AGG + chr6.2:4475827-4475846 None:intergenic 25.0%
! TGATATTCAAGATTTATGGA+AGG - chr6.2:4475916-4475935 MS.gene54738:CDS 25.0%
!!! GATATTTTAGCTATGAGTTT+TGG - chr6.2:4475999-4476018 MS.gene54738:CDS 25.0%
AAAGAAGATTGAGAAATGGA+TGG - chr6.2:4476132-4476151 MS.gene54738:CDS 30.0%
ACACAAAGAAGATTGAGAAA+TGG - chr6.2:4476128-4476147 MS.gene54738:CDS 30.0%
CACAAAAAATCAACAGAACA+AGG + chr6.2:4476376-4476395 None:intergenic 30.0%
GAAATGGATGGAAATTCAAA+AGG - chr6.2:4476144-4476163 MS.gene54738:CDS 30.0%
TTGAAGCTATTGAACATAGA+TGG - chr6.2:4476227-4476246 MS.gene54738:CDS 30.0%
! GTCTAGTTATGAATTTTCTG+AGG - chr6.2:4476021-4476040 MS.gene54738:CDS 30.0%
!!! ATCATTACCTCCTTTTTTGA+TGG - chr6.2:4476189-4476208 MS.gene54738:CDS 30.0%
!!! CTTGTTCTGTTGATTTTTTG+TGG - chr6.2:4476374-4476393 MS.gene54738:CDS 30.0%
!!! GAATTTTCTGAGGTATTCAA+AGG - chr6.2:4476031-4476050 MS.gene54738:CDS 30.0%
CCAAAAACCATCAAAAAAGG+AGG + chr6.2:4476199-4476218 None:intergenic 35.0%
CCACCAAAAACCATCAAAAA+AGG + chr6.2:4476202-4476221 None:intergenic 35.0%
CTCCAATGTAACAAACTAAC+TGG + chr6.2:4476313-4476332 None:intergenic 35.0%
GAACATAGATGGAATCAACA+TGG - chr6.2:4476238-4476257 MS.gene54738:CDS 35.0%
GAGTTTACTAGGATTGTTCA+TGG - chr6.2:4475707-4475726 MS.gene54738:CDS 35.0%
GGAAATTCAAAAGGTGAGAA+GGG - chr6.2:4476153-4476172 MS.gene54738:CDS 35.0%
TGAGGCATATAGAGATCATA+AGG + chr6.2:4476403-4476422 None:intergenic 35.0%
TGGAAATTCAAAAGGTGAGA+AGG - chr6.2:4476152-4476171 MS.gene54738:CDS 35.0%
TGTTACATTGGAGTGGTAAA+GGG - chr6.2:4476320-4476339 MS.gene54738:CDS 35.0%
TTATTTCAACACTGGTGTGA+TGG - chr6.2:4476072-4476091 MS.gene54738:CDS 35.0%
TTGTTACATTGGAGTGGTAA+AGG - chr6.2:4476319-4476338 MS.gene54738:CDS 35.0%
! ACACCAGTGTTGAAATAACA+TGG + chr6.2:4476070-4476089 None:intergenic 35.0%
! TCAAGTGCTTGTCTTATTGA+AGG + chr6.2:4475800-4475819 None:intergenic 35.0%
!! ATGCCATGTTATTTCAACAC+TGG - chr6.2:4476064-4476083 MS.gene54738:CDS 35.0%
!! GTCCAGTTAGTTTGTTACAT+TGG - chr6.2:4476308-4476327 MS.gene54738:CDS 35.0%
!! GTTAGTTTGTTACATTGGAG+TGG - chr6.2:4476313-4476332 MS.gene54738:CDS 35.0%
!! TGGTAATGGATTTGGTGAAA+TGG - chr6.2:4476092-4476111 MS.gene54738:CDS 35.0%
!!! CCTCCTTTTTTGATGGTTTT+TGG - chr6.2:4476196-4476215 MS.gene54738:CDS 35.0%
!!! CCTTTTTTGATGGTTTTTGG+TGG - chr6.2:4476199-4476218 MS.gene54738:CDS 35.0%
AGATGGAATCAACATGGTCT+TGG - chr6.2:4476244-4476263 MS.gene54738:CDS 40.0%
AGTTGTAGAAGTTTGCATCC+TGG - chr6.2:4476286-4476305 MS.gene54738:CDS 40.0%
ATTGCATCAAGTCTTCTCCA+TGG + chr6.2:4476349-4476368 None:intergenic 40.0%
GATCTCTATATGCCTCATGT+GGG - chr6.2:4476406-4476425 MS.gene54738:CDS 40.0%
GTGAGAAGGGTTTATAAGCT+TGG - chr6.2:4476166-4476185 MS.gene54738:CDS 40.0%
TATATGCCTCATGTGGGATA+TGG - chr6.2:4476412-4476431 MS.gene54738:CDS 40.0%
TGATCTCTATATGCCTCATG+TGG - chr6.2:4476405-4476424 MS.gene54738:CDS 40.0%
TGTAACAAACTAACTGGACC+AGG + chr6.2:4476307-4476326 None:intergenic 40.0%
!! GATTTGGTGAAATGGAGAGA+AGG - chr6.2:4476100-4476119 MS.gene54738:CDS 40.0%
!! TGGTGTGATGGTAATGGATT+TGG - chr6.2:4476084-4476103 MS.gene54738:CDS 40.0%
!! TTGACCGATTCGAAGGTAAT+CGG - chr6.2:4475944-4475963 MS.gene54738:CDS 40.0%
CAACACTGGTGTGATGGTAA+TGG - chr6.2:4476078-4476097 MS.gene54738:CDS 45.0%
GAATGTCCATATCCCACATG+AGG + chr6.2:4476421-4476440 None:intergenic 45.0%
! TGGAATCAACATGGTCTTGG+TGG - chr6.2:4476247-4476266 MS.gene54738:CDS 45.0%
!! TGCACCGATTACCTTCGAAT+CGG + chr6.2:4475951-4475970 None:intergenic 45.0%
GGAGTGGTAAAGGGAAACCA+TGG - chr6.2:4476329-4476348 MS.gene54738:CDS 50.0%
TTCGAAGGTAATCGGTGCAC+CGG - chr6.2:4475952-4475971 MS.gene54738:CDS 50.0%
!! GGTTTCGTTGACCGATTCGA+AGG - chr6.2:4475937-4475956 MS.gene54738:CDS 50.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 4475611 4476450 4475611 ID=MS.gene54738
chr6.2 mRNA 4475611 4476450 4475611 ID=MS.gene54738.t1;Parent=MS.gene54738
chr6.2 exon 4475611 4476450 4475611 ID=MS.gene54738.t1.exon1;Parent=MS.gene54738.t1
chr6.2 CDS 4475611 4476450 4475611 ID=cds.MS.gene54738.t1;Parent=MS.gene54738.t1
Gene Sequence

>MS.gene54738

ATGGCTGCAATTCATTCTGTTCTTAAACACACTTCATGTCCTAAGAATTTGTTTTTTCATTTCATTGCATCAGATTCAAGATTAGAGAATAAAGATGAGTTTACTAGGATTGTTCATGGTTCATTTCCTTCATTGAAATTCAAAGTTTATGTCTTCAATGAAATTTTAGTTGAGAATCTTATATCTCCTTCAATAAGACAAGCACTTGAGAATCCTTTGAATTATGCAAGAAGTTATCTTGCTGATTTGCTTGAAGAATGTGTTGAGAGAGTTATCTATTTAGATTCTGATGTTATAGTTGTTGATGATATTCAAGATTTATGGAAGGTTTCGTTGACCGATTCGAAGGTAATCGGTGCACCGGAATATTGTCATGCAAATTTCACAAGATATTTTAGCTATGAGTTTTGGTCTAGTTATGAATTTTCTGAGGTATTCAAAGGAAGAAAAAATATGCCATGTTATTTCAACACTGGTGTGATGGTAATGGATTTGGTGAAATGGAGAGAAGGTGAATACACAAAGAAGATTGAGAAATGGATGGAAATTCAAAAGGTGAGAAGGGTTTATAAGCTTGGATCATTACCTCCTTTTTTGATGGTTTTTGGTGGTGATATTGAAGCTATTGAACATAGATGGAATCAACATGGTCTTGGTGGTGATAATGTTGTTGATAGTTGTAGAAGTTTGCATCCTGGTCCAGTTAGTTTGTTACATTGGAGTGGTAAAGGGAAACCATGGAGAAGACTTGATGCAATGAGACCTTGTTCTGTTGATTTTTTGTGGAAACCTTATGATCTCTATATGCCTCATGTGGGATATGGACATTCATTAATTTAG

Protein sequence

>MS.gene54738.t1

MAAIHSVLKHTSCPKNLFFHFIASDSRLENKDEFTRIVHGSFPSLKFKVYVFNEILVENLISPSIRQALENPLNYARSYLADLLEECVERVIYLDSDVIVVDDIQDLWKVSLTDSKVIGAPEYCHANFTRYFSYEFWSSYEFSEVFKGRKNMPCYFNTGVMVMDLVKWREGEYTKKIEKWMEIQKVRRVYKLGSLPPFLMVFGGDIEAIEHRWNQHGLGGDNVVDSCRSLHPGPVSLLHWSGKGKPWRRLDAMRPCSVDFLWKPYDLYMPHVGYGHSLI