Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56471.t1 | AES90005.1 | 98.9 | 89 | 1 | 0 | 1 | 89 | 1 | 89 | 6.10E-06 | 60.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56471.t1 | G7JHI8 | 98.9 | 89 | 1 | 0 | 1 | 89 | 1 | 89 | 4.4e-06 | 60.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene56471 | 0.808553 | 2.93E-50 | -1.69E-46 |
| MS.gene049915 | MS.gene56471 | 0.804016 | 2.64E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene56471 | 0.818344 | 2.06E-52 | -1.69E-46 |
| MS.gene050642 | MS.gene56471 | 0.826327 | 2.88E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene56471 | 0.811228 | 7.78E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene56471 | 0.818858 | 1.57E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene56471 | 0.837823 | 4.14E-57 | -1.69E-46 |
| MS.gene052376 | MS.gene56471 | 0.863535 | 2.32E-64 | -1.69E-46 |
| MS.gene054983 | MS.gene56471 | 0.801659 | 8.11E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene56471 | 0.805101 | 1.57E-49 | -1.69E-46 |
| MS.gene059249 | MS.gene56471 | 0.800055 | 1.72E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56471.t1 | MTR_4g083130 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 1.19e-57 | 172 |
| MS.gene56471.t1 | MTR_4g083110 | 80.899 | 89 | 13 | 1 | 1 | 89 | 1 | 85 | 6.38e-40 | 127 |
| MS.gene56471.t1 | MTR_5g077640 | 77.528 | 89 | 20 | 0 | 1 | 89 | 1 | 89 | 3.68e-36 | 117 |
| MS.gene56471.t1 | MTR_5g077680 | 78.846 | 52 | 11 | 0 | 1 | 52 | 1 | 52 | 1.30e-23 | 85.5 |
| MS.gene56471.t1 | MTR_8g079458 | 78.846 | 52 | 11 | 0 | 1 | 52 | 1 | 52 | 1.30e-23 | 85.5 |
| MS.gene56471.t1 | MTR_4g012020 | 76.923 | 52 | 12 | 0 | 1 | 52 | 1 | 52 | 5.15e-23 | 84.0 |
| MS.gene56471.t1 | MTR_1437s0010 | 75.000 | 52 | 13 | 0 | 1 | 52 | 1 | 52 | 2.05e-22 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56471.t1 | AT1G30260 | 60.417 | 48 | 18 | 1 | 1 | 48 | 1 | 47 | 3.59e-15 | 65.1 |
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATGCAGTTGTTAATTCT+AGG | 0.257861 | 4.4:+29958133 | MS.gene56471:CDS |
| AACAATCACTGAAGAGGATA+TGG | 0.331678 | 4.4:+29958026 | MS.gene56471:CDS |
| GTGTTCTATGAACTTCCTTC+AGG | 0.381163 | 4.4:-29958161 | None:intergenic |
| GCGTAGAACATCAAAGATGA+TGG | 0.510830 | 4.4:+29958002 | MS.gene56471:CDS |
| CCTAGAATTAACAACTGCAT+TGG | 0.551602 | 4.4:-29958133 | None:intergenic |
| AACAACTGCATTGGTAGAAG+AGG | 0.557920 | 4.4:-29958124 | None:intergenic |
| TGGCGAATTCTCGCATTGCA+AGG | 0.566271 | 4.4:+29957935 | MS.gene56471:CDS |
| CACAGTATGTTAGCGTGATG+AGG | 0.569645 | 4.4:+29957980 | MS.gene56471:CDS |
| TAATTCTAGGTACTTCCTGA+AGG | 0.589595 | 4.4:+29958146 | MS.gene56471:CDS |
| GATGGAAACAATCACTGAAG+AGG | 0.591147 | 4.4:+29958020 | MS.gene56471:CDS |
| CTCATCACGCTAACATACTG+TGG | 0.654266 | 4.4:-29957979 | None:intergenic |
| ATCACGCTAACATACTGTGG+TGG | 0.690677 | 4.4:-29957976 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATGAAGTTGATGAACTTTT+AGG | - | chr4.4:29958084-29958103 | None:intergenic | 30.0% |
| AACAATCACTGAAGAGGATA+TGG | + | chr4.4:29958026-29958045 | MS.gene56471:CDS | 35.0% | |
| CCAATGCAGTTGTTAATTCT+AGG | + | chr4.4:29958133-29958152 | MS.gene56471:CDS | 35.0% | |
| CCTAGAATTAACAACTGCAT+TGG | - | chr4.4:29958136-29958155 | None:intergenic | 35.0% | |
| TAATTCTAGGTACTTCCTGA+AGG | + | chr4.4:29958146-29958165 | MS.gene56471:CDS | 35.0% | |
| !!! | GAAGTTGATGAACTTTTAGG+TGG | - | chr4.4:29958081-29958100 | None:intergenic | 35.0% |
| GATGGAAACAATCACTGAAG+AGG | + | chr4.4:29958020-29958039 | MS.gene56471:CDS | 40.0% | |
| GCGTAGAACATCAAAGATGA+TGG | + | chr4.4:29958002-29958021 | MS.gene56471:CDS | 40.0% | |
| GTGTTCTATGAACTTCCTTC+AGG | - | chr4.4:29958164-29958183 | None:intergenic | 40.0% | |
| ! | AACAACTGCATTGGTAGAAG+AGG | - | chr4.4:29958127-29958146 | None:intergenic | 40.0% |
| ! | CATTGCAAGGTTTTTCATGG+AGG | + | chr4.4:29957948-29957967 | MS.gene56471:CDS | 40.0% |
| ! | TCGCATTGCAAGGTTTTTCA+TGG | + | chr4.4:29957945-29957964 | MS.gene56471:CDS | 40.0% |
| !! | GTTGATGAACTTTTAGGTGG+TGG | - | chr4.4:29958078-29958097 | None:intergenic | 40.0% |
| ATCACGCTAACATACTGTGG+TGG | - | chr4.4:29957979-29957998 | None:intergenic | 45.0% | |
| CACAGTATGTTAGCGTGATG+AGG | + | chr4.4:29957980-29957999 | MS.gene56471:CDS | 45.0% | |
| CTCATCACGCTAACATACTG+TGG | - | chr4.4:29957982-29958001 | None:intergenic | 45.0% | |
| ! | TGCAAGGTTTTTCATGGAGG+TGG | + | chr4.4:29957951-29957970 | MS.gene56471:CDS | 45.0% |
| TGGCGAATTCTCGCATTGCA+AGG | + | chr4.4:29957935-29957954 | MS.gene56471:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29957934 | 29958203 | 29957934 | ID=MS.gene56471 |
| chr4.4 | mRNA | 29957934 | 29958203 | 29957934 | ID=MS.gene56471.t1;Parent=MS.gene56471 |
| chr4.4 | exon | 29957934 | 29958203 | 29957934 | ID=MS.gene56471.t1.exon1;Parent=MS.gene56471.t1 |
| chr4.4 | CDS | 29957934 | 29958203 | 29957934 | ID=cds.MS.gene56471.t1;Parent=MS.gene56471.t1 |
| Gene Sequence |
| Protein sequence |
